Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene20172.t1 | XP_003630627.1 | 98.6 | 437 | 6 | 0 | 6 | 442 | 1 | 437 | 1.70E-245 | 858.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene20172.t1 | Q8VYG2 | 65.0 | 432 | 140 | 2 | 11 | 442 | 3 | 423 | 5.2e-155 | 548.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene20172.t1 | G7LJJ0 | 98.6 | 437 | 6 | 0 | 6 | 442 | 1 | 437 | 1.2e-245 | 858.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049178 | MS.gene20172 | 0.800089 | 1.70E-48 | -1.69E-46 |
| MS.gene049887 | MS.gene20172 | 0.880326 | 6.02E-70 | -1.69E-46 |
| MS.gene050838 | MS.gene20172 | 0.822797 | 1.95E-53 | -1.69E-46 |
| MS.gene051885 | MS.gene20172 | 0.827472 | 1.54E-54 | -1.69E-46 |
| MS.gene052732 | MS.gene20172 | 0.814065 | 1.86E-51 | -1.69E-46 |
| MS.gene053144 | MS.gene20172 | 0.849661 | 2.80E-60 | -1.69E-46 |
| MS.gene054584 | MS.gene20172 | 0.83049 | 2.85E-55 | -1.69E-46 |
| MS.gene054824 | MS.gene20172 | 0.803607 | 3.22E-49 | -1.69E-46 |
| MS.gene055465 | MS.gene20172 | 0.802406 | 5.70E-49 | -1.69E-46 |
| MS.gene055468 | MS.gene20172 | 0.829311 | 5.53E-55 | -1.69E-46 |
| MS.gene055469 | MS.gene20172 | 0.829413 | 5.22E-55 | -1.69E-46 |
| MS.gene056043 | MS.gene20172 | -0.801531 | 8.62E-49 | -1.69E-46 |
| MS.gene056432 | MS.gene20172 | 0.80923 | 2.10E-50 | -1.69E-46 |
| MS.gene058639 | MS.gene20172 | 0.808353 | 3.23E-50 | -1.69E-46 |
| MS.gene058852 | MS.gene20172 | 0.834402 | 3.06E-56 | -1.69E-46 |
| MS.gene059171 | MS.gene20172 | 0.834272 | 3.30E-56 | -1.69E-46 |
| MS.gene061006 | MS.gene20172 | 0.804737 | 1.87E-49 | -1.69E-46 |
| MS.gene061007 | MS.gene20172 | 0.814218 | 1.72E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene20172.t1 | MTR_8g101540 | 98.627 | 437 | 6 | 0 | 6 | 442 | 1 | 437 | 0.0 | 897 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene20172.t1 | AT3G10700 | 64.815 | 432 | 141 | 2 | 11 | 442 | 3 | 423 | 0.0 | 548 |
| MS.gene20172.t1 | AT3G10700 | 63.812 | 362 | 120 | 2 | 81 | 442 | 5 | 355 | 2.45e-154 | 441 |
| MS.gene20172.t1 | AT3G10700 | 64.907 | 322 | 102 | 2 | 11 | 332 | 3 | 313 | 6.31e-138 | 398 |
Find 102 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTTGATTGAGAATGAATATT+TGG | 0.148149 | 4.4:-3953625 | MS.gene20172:CDS |
| CCCTATCGAATTTGTCCTTT+GGG | 0.178205 | 4.4:-3956491 | MS.gene20172:CDS |
| AAAATGTTGCTGGCTCTTTC+TGG | 0.184400 | 4.4:-3952212 | MS.gene20172:CDS |
| ACGGGAAGTTTAGAAGATTT+TGG | 0.201422 | 4.4:-3950247 | MS.gene20172:CDS |
| TTGAGAGCTCCTGGTGTATT+GGG | 0.238575 | 4.4:-3948886 | MS.gene20172:CDS |
| GAAATAATGCTCTGCTCTTT+TGG | 0.241028 | 4.4:+3950415 | None:intergenic |
| AGCCACAGGAACAATGTAAT+TGG | 0.256134 | 4.4:-3955364 | MS.gene20172:CDS |
| CTTCTGGGGTTTACTCCTTC+TGG | 0.272838 | 4.4:-3956211 | MS.gene20172:CDS |
| TTTAGCTATGACAATCAATA+AGG | 0.279528 | 4.4:-3956239 | MS.gene20172:CDS |
| GGAATTCTCATCGCTGCTTC+TGG | 0.291132 | 4.4:-3950226 | MS.gene20172:CDS |
| GGGGCACGTTTCAGTGGTGC+TGG | 0.313834 | 4.4:-3948865 | MS.gene20172:CDS |
| ATTGAGAGCTCCTGGTGTAT+TGG | 0.326421 | 4.4:-3948887 | MS.gene20172:CDS |
| AATCAATAAGGGAATACTTC+TGG | 0.338568 | 4.4:-3956227 | MS.gene20172:CDS |
| TTCAAGGAGAAGTGAAGTTC+AGG | 0.341185 | 4.4:-3955890 | MS.gene20172:intron |
| CTACCAACCCCGGATATAAT+AGG | 0.344598 | 4.4:-3952172 | MS.gene20172:CDS |
| GCCCTATCGAATTTGTCCTT+TGG | 0.353442 | 4.4:-3956492 | MS.gene20172:CDS |
| TTGTAGTTTGTAATTCGTTC+AGG | 0.355824 | 4.4:-3955918 | MS.gene20172:intron |
| CCGTATATGCACTACAGAAT+AGG | 0.363557 | 4.4:-3955322 | MS.gene20172:CDS |
| CGTATATGCACTACAGAATA+GGG | 0.363640 | 4.4:-3955321 | MS.gene20172:CDS |
| CAATGTTTACAGGGTATCAT+TGG | 0.367185 | 4.4:-3954461 | MS.gene20172:intron |
| GCCACAGGAACAATGTAATT+GGG | 0.370262 | 4.4:-3955363 | MS.gene20172:CDS |
| TTGATTGAGAATGAATATTT+GGG | 0.370505 | 4.4:-3953624 | MS.gene20172:CDS |
| TTCAGTGGTGCTGGGTTTAG+AGG | 0.370802 | 4.4:-3948856 | MS.gene20172:CDS |
| TCTGATGGTCTGGACAGTTC+AGG | 0.372167 | 4.4:-3954425 | MS.gene20172:CDS |
| CCTATTCTGTAGTGCATATA+CGG | 0.381651 | 4.4:+3955322 | None:intergenic |
| GGAGTCGAGGCTTGGGAAAC+GGG | 0.384982 | 4.4:-3950265 | MS.gene20172:CDS |
| AATTAATTCTGCAGATCATC+TGG | 0.392142 | 4.4:-3951704 | MS.gene20172:intron |
| TGCAACTCGCCTATTATATC+CGG | 0.393949 | 4.4:+3952163 | None:intergenic |
| ATCAATAAGGGAATACTTCT+GGG | 0.405069 | 4.4:-3956226 | MS.gene20172:CDS |
| AGTAGCATACCTCTTAGCTT+TGG | 0.420578 | 4.4:-3954302 | MS.gene20172:CDS |
| GAAAGCAGCTAAAATGTTGC+TGG | 0.429654 | 4.4:-3952222 | MS.gene20172:CDS |
| CTGAAAATATTGAATATGAT+AGG | 0.435242 | 4.4:-3954249 | MS.gene20172:intron |
| ATACACCAGGAGCTCTCAAT+AGG | 0.436295 | 4.4:+3948890 | None:intergenic |
| TTGTGAGCCTCATAAACTTC+CGG | 0.440960 | 4.4:+3951518 | None:intergenic |
| GGGCACGTTTCAGTGGTGCT+GGG | 0.443328 | 4.4:-3948864 | MS.gene20172:CDS |
| TTAGCTATGACAATCAATAA+GGG | 0.443756 | 4.4:-3956238 | MS.gene20172:CDS |
| TCTGACTAGCTAACTCGGGC+TGG | 0.445555 | 4.4:+3948758 | None:intergenic |
| TAATCCAGGAGTCGAGGCTT+GGG | 0.458763 | 4.4:-3950272 | MS.gene20172:intron |
| AGGAGTCGAGGCTTGGGAAA+CGG | 0.463752 | 4.4:-3950266 | MS.gene20172:CDS |
| GAGAGAGAAAGTTTCTCAAA+TGG | 0.464031 | 4.4:-3956552 | MS.gene20172:CDS |
| TGTACTCTTGCACCGTTGGC+CGG | 0.464779 | 4.4:+3952261 | None:intergenic |
| CATACGTGGTTCTGATGGTC+TGG | 0.468452 | 4.4:-3954435 | MS.gene20172:CDS |
| CTGAAGCTGCATCATTTGTC+AGG | 0.475935 | 4.4:-3948798 | MS.gene20172:CDS |
| TTTGTCTTAGTAGTTTGCAC+TGG | 0.480855 | 4.4:+3955416 | None:intergenic |
| GCAACTCGCCTATTATATCC+GGG | 0.484604 | 4.4:+3952164 | None:intergenic |
| AGTTGCACCGGAAGTTTATG+AGG | 0.486774 | 4.4:-3951525 | MS.gene20172:CDS |
| TTGCATGTTCCAAAGCTAAG+AGG | 0.495586 | 4.4:+3954293 | None:intergenic |
| ATTCGTTCAGGACAGTTTCA+AGG | 0.498096 | 4.4:-3955906 | MS.gene20172:CDS |
| GCTTATCTGACTAGCTAACT+CGG | 0.498921 | 4.4:+3948753 | None:intergenic |
| ATTACATTGTTCCTGTGGCT+CGG | 0.509440 | 4.4:+3955367 | None:intergenic |
| TTAATCCAGGAGTCGAGGCT+TGG | 0.509901 | 4.4:-3950273 | MS.gene20172:intron |
| GAGATCCTATTGAGAGCTCC+TGG | 0.513071 | 4.4:-3948895 | MS.gene20172:CDS |
| TGAGAGCTCCTGGTGTATTG+GGG | 0.515368 | 4.4:-3948885 | MS.gene20172:CDS |
| GCGGAGCACATCCTGTCAAA+TGG | 0.519126 | 4.4:-3951671 | MS.gene20172:intron |
| AGAGAATTCTTCCGAGCCAC+AGG | 0.528821 | 4.4:-3955378 | MS.gene20172:CDS |
| CCTATCGAATTTGTCCTTTG+GGG | 0.530180 | 4.4:-3956490 | MS.gene20172:CDS |
| GTGCAAATTAGAACCCGATC+TGG | 0.532694 | 4.4:-3950438 | MS.gene20172:CDS |
| CTATCGAATTTGTCCTTTGG+GGG | 0.532892 | 4.4:-3956489 | MS.gene20172:CDS |
| TCAAATACTGACCATTTGAC+AGG | 0.535076 | 4.4:+3951660 | None:intergenic |
| TTGAAAGTAGTATAGCTGAT+TGG | 0.535673 | 4.4:+3953580 | None:intergenic |
| TCCATTCAAAATTATGAATG+TGG | 0.538112 | 4.4:-3950196 | MS.gene20172:intron |
| TTCTCATCGCTGCTTCTGGT+CGG | 0.543594 | 4.4:-3950222 | MS.gene20172:CDS |
| GAGAATATGCGTGTTATGAA+AGG | 0.544922 | 4.4:-3950389 | MS.gene20172:intron |
| CTGACTAGCTAACTCGGGCT+GGG | 0.547001 | 4.4:+3948759 | None:intergenic |
| AGGATGCTGTATTGCCCTTG+TGG | 0.556238 | 4.4:-3948836 | MS.gene20172:CDS |
| CCCCAATTACATTGTTCCTG+TGG | 0.559811 | 4.4:+3955362 | None:intergenic |
| GCGAGTTGCAGAGTGTAAAG+AGG | 0.567225 | 4.4:-3952150 | MS.gene20172:CDS |
| CCCAAAGGACAAATTCGATA+GGG | 0.568698 | 4.4:+3956491 | None:intergenic |
| AGAAGATGGCAGGGTCGTGT+TGG | 0.579641 | 4.4:-3957029 | MS.gene20172:CDS |
| GAAAGTTTCTCAAATGGCAA+AGG | 0.583814 | 4.4:-3956546 | MS.gene20172:CDS |
| CCACAGGAACAATGTAATTG+GGG | 0.584095 | 4.4:-3955362 | MS.gene20172:CDS |
| GGATACATACGTGGTTCTGA+TGG | 0.587638 | 4.4:-3954440 | MS.gene20172:CDS |
| TTCACCTCATCGCTTCCAGA+AGG | 0.589446 | 4.4:+3956196 | None:intergenic |
| ATCTCATACAGTTGAATCAG+TGG | 0.591158 | 4.4:+3948913 | None:intergenic |
| CTTATCTGACTAGCTAACTC+GGG | 0.593706 | 4.4:+3948754 | None:intergenic |
| TCGCCTATTATATCCGGGGT+TGG | 0.597810 | 4.4:+3952169 | None:intergenic |
| TAACGTACGTACCTCTGTAT+CGG | 0.598872 | 4.4:+3957002 | None:intergenic |
| TAGGGGACACAATATTTCAA+AGG | 0.601696 | 4.4:-3955303 | MS.gene20172:intron |
| TTTCTTCCAAGCAGTTGCAC+CGG | 0.608122 | 4.4:-3951537 | MS.gene20172:intron |
| TAGCAAGGTGCTCCTCCACA+AGG | 0.611891 | 4.4:+3948821 | None:intergenic |
| AATGTTTAATCCAGGAGTCG+AGG | 0.613521 | 4.4:-3950278 | MS.gene20172:intron |
| AAGAATATAAGCTTATTCAC+CGG | 0.614977 | 4.4:-3952280 | MS.gene20172:CDS |
| CCCCAAAGGACAAATTCGAT+AGG | 0.615725 | 4.4:+3956490 | None:intergenic |
| GAGAGCTCCTGGTGTATTGG+GGG | 0.618129 | 4.4:-3948884 | MS.gene20172:CDS |
| ATGATGCAGCTTCAGTAGCA+AGG | 0.619433 | 4.4:+3948806 | None:intergenic |
| TTTGATGTGCATGAATTGCA+AGG | 0.621600 | 4.4:-3953547 | MS.gene20172:intron |
| ATGCTGTATTGCCCTTGTGG+AGG | 0.622412 | 4.4:-3948833 | MS.gene20172:CDS |
| TACTCCTTCTGGAAGCGATG+AGG | 0.627318 | 4.4:-3956200 | MS.gene20172:intron |
| GGAAGTTTATGAGGCTCACA+AGG | 0.632515 | 4.4:-3951516 | MS.gene20172:intron |
| GCTATACTACTTTCAAGACA+TGG | 0.632611 | 4.4:-3953573 | MS.gene20172:CDS |
| GTACGTACCTCTGTATCGGA+AGG | 0.632909 | 4.4:+3957006 | None:intergenic |
| TCAATAAGGGAATACTTCTG+GGG | 0.643607 | 4.4:-3956225 | MS.gene20172:CDS |
| CAACTCGCCTATTATATCCG+GGG | 0.650891 | 4.4:+3952165 | None:intergenic |
| TAAGCTTATTCACCGGCCAA+CGG | 0.652544 | 4.4:-3952273 | MS.gene20172:CDS |
| ATCATCTGGAGATTATGAAG+CGG | 0.659188 | 4.4:-3951690 | MS.gene20172:CDS |
| CAAGCTTTGACTACCAACCC+CGG | 0.668548 | 4.4:-3952182 | MS.gene20172:CDS |
| GTGTTGGCCTTCCGATACAG+AGG | 0.670297 | 4.4:-3957013 | MS.gene20172:intron |
| CACAGGAACAATGTAATTGG+GGG | 0.671775 | 4.4:-3955361 | MS.gene20172:CDS |
| AGCAAGGTGCTCCTCCACAA+GGG | 0.672873 | 4.4:+3948822 | None:intergenic |
| TGTCAGGAGAGAATATCTCA+AGG | 0.688973 | 4.4:-3948782 | MS.gene20172:CDS |
| GTATATGCACTACAGAATAG+GGG | 0.695498 | 4.4:-3955320 | MS.gene20172:CDS |
| GGTATCATTGGATACATACG+TGG | 0.735502 | 4.4:-3954449 | MS.gene20172:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.4 | gene | 3948699 | 3957294 | 3948699 | ID=MS.gene20172 |
| chr4.4 | mRNA | 3948699 | 3957294 | 3948699 | ID=MS.gene20172.t1;Parent=MS.gene20172 |
| chr4.4 | exon | 3957292 | 3957294 | 3957292 | ID=MS.gene20172.t1.exon1;Parent=MS.gene20172.t1 |
| chr4.4 | CDS | 3957292 | 3957294 | 3957292 | ID=cds.MS.gene20172.t1;Parent=MS.gene20172.t1 |
| chr4.4 | exon | 3957014 | 3957058 | 3957014 | ID=MS.gene20172.t1.exon2;Parent=MS.gene20172.t1 |
| chr4.4 | CDS | 3957014 | 3957058 | 3957014 | ID=cds.MS.gene20172.t1;Parent=MS.gene20172.t1 |
| chr4.4 | exon | 3956472 | 3956585 | 3956472 | ID=MS.gene20172.t1.exon3;Parent=MS.gene20172.t1 |
| chr4.4 | CDS | 3956472 | 3956585 | 3956472 | ID=cds.MS.gene20172.t1;Parent=MS.gene20172.t1 |
| chr4.4 | exon | 3956201 | 3956272 | 3956201 | ID=MS.gene20172.t1.exon4;Parent=MS.gene20172.t1 |
| chr4.4 | CDS | 3956201 | 3956272 | 3956201 | ID=cds.MS.gene20172.t1;Parent=MS.gene20172.t1 |
| chr4.4 | exon | 3955891 | 3955934 | 3955891 | ID=MS.gene20172.t1.exon5;Parent=MS.gene20172.t1 |
| chr4.4 | CDS | 3955891 | 3955934 | 3955891 | ID=cds.MS.gene20172.t1;Parent=MS.gene20172.t1 |
| chr4.4 | exon | 3955304 | 3955457 | 3955304 | ID=MS.gene20172.t1.exon6;Parent=MS.gene20172.t1 |
| chr4.4 | CDS | 3955304 | 3955457 | 3955304 | ID=cds.MS.gene20172.t1;Parent=MS.gene20172.t1 |
| chr4.4 | exon | 3954406 | 3954471 | 3954406 | ID=MS.gene20172.t1.exon7;Parent=MS.gene20172.t1 |
| chr4.4 | CDS | 3954406 | 3954471 | 3954406 | ID=cds.MS.gene20172.t1;Parent=MS.gene20172.t1 |
| chr4.4 | exon | 3954250 | 3954329 | 3954250 | ID=MS.gene20172.t1.exon8;Parent=MS.gene20172.t1 |
| chr4.4 | CDS | 3954250 | 3954329 | 3954250 | ID=cds.MS.gene20172.t1;Parent=MS.gene20172.t1 |
| chr4.4 | exon | 3953548 | 3953647 | 3953548 | ID=MS.gene20172.t1.exon9;Parent=MS.gene20172.t1 |
| chr4.4 | CDS | 3953548 | 3953647 | 3953548 | ID=cds.MS.gene20172.t1;Parent=MS.gene20172.t1 |
| chr4.4 | exon | 3952131 | 3952306 | 3952131 | ID=MS.gene20172.t1.exon10;Parent=MS.gene20172.t1 |
| chr4.4 | CDS | 3952131 | 3952306 | 3952131 | ID=cds.MS.gene20172.t1;Parent=MS.gene20172.t1 |
| chr4.4 | exon | 3951672 | 3951712 | 3951672 | ID=MS.gene20172.t1.exon11;Parent=MS.gene20172.t1 |
| chr4.4 | CDS | 3951672 | 3951712 | 3951672 | ID=cds.MS.gene20172.t1;Parent=MS.gene20172.t1 |
| chr4.4 | exon | 3951517 | 3951545 | 3951517 | ID=MS.gene20172.t1.exon12;Parent=MS.gene20172.t1 |
| chr4.4 | CDS | 3951517 | 3951545 | 3951517 | ID=cds.MS.gene20172.t1;Parent=MS.gene20172.t1 |
| chr4.4 | exon | 3950390 | 3950459 | 3950390 | ID=MS.gene20172.t1.exon13;Parent=MS.gene20172.t1 |
| chr4.4 | CDS | 3950390 | 3950459 | 3950390 | ID=cds.MS.gene20172.t1;Parent=MS.gene20172.t1 |
| chr4.4 | exon | 3950197 | 3950286 | 3950197 | ID=MS.gene20172.t1.exon14;Parent=MS.gene20172.t1 |
| chr4.4 | CDS | 3950197 | 3950286 | 3950197 | ID=cds.MS.gene20172.t1;Parent=MS.gene20172.t1 |
| chr4.4 | exon | 3948699 | 3948943 | 3948699 | ID=MS.gene20172.t1.exon15;Parent=MS.gene20172.t1 |
| chr4.4 | CDS | 3948699 | 3948943 | 3948699 | ID=cds.MS.gene20172.t1;Parent=MS.gene20172.t1 |
| Gene Sequence |
| Protein sequence |
