Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene20413.t1 | XP_013452524.1 | 90.2 | 327 | 31 | 1 | 1 | 326 | 1 | 327 | 2.40E-169 | 604.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene20413.t1 | Q0ZPV7 | 53.0 | 330 | 147 | 3 | 1 | 325 | 1 | 327 | 1.7e-94 | 347.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene20413.t1 | A0A072UA80 | 90.2 | 327 | 31 | 1 | 1 | 326 | 1 | 327 | 1.8e-169 | 604.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene019398 | MS.gene20413 | PPI |
| MS.gene37505 | MS.gene20413 | PPI |
| MS.gene23638 | MS.gene20413 | PPI |
| MS.gene87835 | MS.gene20413 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene20413.t1 | MTR_6g464510 | 91.743 | 327 | 26 | 1 | 1 | 326 | 1 | 327 | 0.0 | 623 |
| MS.gene20413.t1 | MTR_6g464280 | 64.350 | 331 | 107 | 5 | 1 | 326 | 1 | 325 | 2.96e-153 | 432 |
| MS.gene20413.t1 | MTR_6g464320 | 64.350 | 331 | 108 | 4 | 1 | 326 | 1 | 326 | 8.70e-152 | 429 |
| MS.gene20413.t1 | MTR_1g102160 | 54.407 | 329 | 142 | 3 | 1 | 325 | 1 | 325 | 2.97e-125 | 362 |
| MS.gene20413.t1 | MTR_1g102170 | 52.077 | 313 | 148 | 1 | 12 | 324 | 27 | 337 | 3.24e-117 | 342 |
| MS.gene20413.t1 | MTR_6g464120 | 53.503 | 314 | 141 | 4 | 7 | 318 | 8 | 318 | 1.18e-111 | 334 |
| MS.gene20413.t1 | MTR_8g035520 | 49.541 | 327 | 148 | 6 | 9 | 326 | 7 | 325 | 8.94e-104 | 307 |
| MS.gene20413.t1 | MTR_0007s0520 | 49.032 | 310 | 145 | 5 | 23 | 325 | 21 | 324 | 1.43e-99 | 296 |
| MS.gene20413.t1 | MTR_8g035540 | 48.137 | 322 | 150 | 8 | 15 | 325 | 1 | 316 | 2.17e-98 | 293 |
| MS.gene20413.t1 | MTR_8g035500 | 40.752 | 319 | 176 | 5 | 15 | 325 | 4 | 317 | 6.22e-82 | 251 |
| MS.gene20413.t1 | MTR_1g111280 | 35.103 | 339 | 193 | 8 | 4 | 326 | 16 | 343 | 2.49e-59 | 194 |
| MS.gene20413.t1 | MTR_7g406880 | 35.690 | 297 | 165 | 9 | 52 | 325 | 56 | 349 | 2.79e-51 | 173 |
| MS.gene20413.t1 | MTR_6g065400 | 35.906 | 298 | 160 | 9 | 27 | 300 | 31 | 321 | 1.44e-48 | 166 |
| MS.gene20413.t1 | MTR_6g065430 | 35.738 | 305 | 183 | 8 | 27 | 325 | 30 | 327 | 3.79e-48 | 165 |
| MS.gene20413.t1 | MTR_0007s0480 | 49.711 | 173 | 76 | 4 | 160 | 325 | 2 | 170 | 1.11e-47 | 158 |
| MS.gene20413.t1 | MTR_6g065110 | 38.017 | 242 | 137 | 5 | 27 | 259 | 32 | 269 | 2.10e-47 | 163 |
| MS.gene20413.t1 | MTR_6g065120 | 34.395 | 314 | 187 | 8 | 25 | 326 | 30 | 336 | 3.61e-47 | 162 |
| MS.gene20413.t1 | MTR_6g065370 | 34.228 | 298 | 165 | 8 | 27 | 300 | 31 | 321 | 3.88e-46 | 159 |
| MS.gene20413.t1 | MTR_6g065270 | 33.968 | 315 | 186 | 10 | 27 | 326 | 29 | 336 | 4.70e-45 | 157 |
| MS.gene20413.t1 | MTR_4g086510 | 35.390 | 308 | 172 | 10 | 31 | 325 | 24 | 317 | 1.28e-44 | 155 |
| MS.gene20413.t1 | MTR_7g056207 | 36.900 | 271 | 139 | 10 | 31 | 278 | 56 | 317 | 3.44e-43 | 152 |
| MS.gene20413.t1 | MTR_7g107040 | 33.117 | 308 | 187 | 9 | 27 | 325 | 19 | 316 | 7.94e-43 | 150 |
| MS.gene20413.t1 | MTR_4g050140 | 34.043 | 282 | 175 | 6 | 51 | 325 | 38 | 315 | 1.49e-42 | 149 |
| MS.gene20413.t1 | MTR_3g101420 | 33.735 | 332 | 185 | 10 | 8 | 325 | 4 | 314 | 2.83e-41 | 146 |
| MS.gene20413.t1 | MTR_6g065340 | 33.333 | 309 | 165 | 9 | 1 | 291 | 8 | 293 | 2.56e-40 | 144 |
| MS.gene20413.t1 | MTR_1g012430 | 34.074 | 270 | 159 | 9 | 27 | 291 | 15 | 270 | 2.39e-39 | 141 |
| MS.gene20413.t1 | MTR_4g086480 | 31.699 | 306 | 183 | 8 | 31 | 325 | 28 | 318 | 1.62e-38 | 139 |
| MS.gene20413.t1 | MTR_1g089310 | 34.310 | 239 | 136 | 4 | 22 | 246 | 32 | 263 | 4.17e-37 | 136 |
| MS.gene20413.t1 | MTR_1g089310 | 34.310 | 239 | 136 | 4 | 22 | 246 | 48 | 279 | 4.67e-37 | 136 |
| MS.gene20413.t1 | MTR_7g093950 | 32.917 | 240 | 142 | 4 | 22 | 246 | 32 | 267 | 2.78e-35 | 131 |
| MS.gene20413.t1 | MTR_4g086540 | 29.766 | 299 | 179 | 7 | 28 | 324 | 31 | 300 | 3.33e-35 | 130 |
| MS.gene20413.t1 | MTR_3g450680 | 31.562 | 320 | 182 | 12 | 27 | 325 | 28 | 331 | 1.68e-32 | 123 |
| MS.gene20413.t1 | MTR_4g086470 | 32.171 | 258 | 157 | 6 | 39 | 291 | 35 | 279 | 1.79e-32 | 123 |
| MS.gene20413.t1 | MTR_1g082210 | 32.231 | 242 | 137 | 8 | 22 | 246 | 32 | 263 | 6.81e-32 | 122 |
| MS.gene20413.t1 | MTR_3g012440 | 35.789 | 190 | 114 | 3 | 67 | 250 | 73 | 260 | 3.19e-30 | 117 |
| MS.gene20413.t1 | MTR_1g105020 | 31.090 | 312 | 191 | 9 | 28 | 325 | 21 | 322 | 6.48e-30 | 116 |
| MS.gene20413.t1 | MTR_5g005850 | 29.747 | 316 | 191 | 12 | 28 | 325 | 26 | 328 | 1.44e-29 | 115 |
| MS.gene20413.t1 | MTR_8g040860 | 30.581 | 327 | 184 | 14 | 22 | 325 | 22 | 328 | 1.76e-28 | 112 |
| MS.gene20413.t1 | MTR_5g005920 | 25.155 | 322 | 212 | 11 | 22 | 325 | 20 | 330 | 1.40e-27 | 110 |
| MS.gene20413.t1 | MTR_5g005900 | 29.224 | 219 | 140 | 5 | 27 | 235 | 21 | 234 | 2.91e-26 | 106 |
| MS.gene20413.t1 | MTR_6g022710 | 30.800 | 250 | 128 | 4 | 30 | 235 | 98 | 346 | 7.31e-26 | 107 |
| MS.gene20413.t1 | MTR_1g104930 | 38.172 | 186 | 97 | 6 | 27 | 203 | 24 | 200 | 8.08e-25 | 102 |
| MS.gene20413.t1 | MTR_7g051020 | 29.874 | 318 | 188 | 11 | 27 | 325 | 24 | 325 | 3.28e-23 | 98.2 |
| MS.gene20413.t1 | MTR_1g104933 | 36.943 | 157 | 88 | 3 | 54 | 203 | 46 | 198 | 3.13e-18 | 84.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene20413.t1 | AT5G62180 | 48.137 | 322 | 162 | 2 | 9 | 325 | 2 | 323 | 1.33e-110 | 325 |
| MS.gene20413.t1 | AT2G45600 | 46.646 | 328 | 152 | 6 | 7 | 325 | 2 | 315 | 1.80e-98 | 294 |
| MS.gene20413.t1 | AT2G45610 | 43.769 | 329 | 169 | 7 | 7 | 326 | 3 | 324 | 1.48e-87 | 266 |
| MS.gene20413.t1 | AT5G16080 | 35.448 | 268 | 151 | 6 | 37 | 294 | 50 | 305 | 2.79e-47 | 162 |
| MS.gene20413.t1 | AT1G68620 | 40.000 | 210 | 118 | 6 | 42 | 245 | 46 | 253 | 1.67e-46 | 160 |
| MS.gene20413.t1 | AT5G23530 | 33.564 | 289 | 177 | 7 | 40 | 325 | 50 | 326 | 1.46e-41 | 147 |
| MS.gene20413.t1 | AT5G06570 | 35.889 | 287 | 165 | 9 | 51 | 325 | 46 | 325 | 1.73e-38 | 139 |
| MS.gene20413.t1 | AT5G06570 | 35.889 | 287 | 165 | 9 | 51 | 325 | 46 | 325 | 1.73e-38 | 139 |
| MS.gene20413.t1 | AT5G06570 | 35.889 | 287 | 165 | 9 | 51 | 325 | 61 | 340 | 2.13e-38 | 139 |
| MS.gene20413.t1 | AT5G06570 | 35.889 | 287 | 165 | 9 | 51 | 325 | 61 | 340 | 2.13e-38 | 139 |
| MS.gene20413.t1 | AT3G63010 | 41.954 | 174 | 93 | 3 | 74 | 246 | 98 | 264 | 3.10e-37 | 137 |
| MS.gene20413.t1 | AT1G49640 | 37.500 | 192 | 111 | 5 | 20 | 207 | 10 | 196 | 3.53e-36 | 133 |
| MS.gene20413.t1 | AT3G48690 | 34.686 | 271 | 158 | 10 | 31 | 291 | 21 | 282 | 4.81e-36 | 132 |
| MS.gene20413.t1 | AT1G19190 | 30.645 | 310 | 189 | 9 | 28 | 325 | 18 | 313 | 2.60e-35 | 130 |
| MS.gene20413.t1 | AT1G47480 | 30.225 | 311 | 192 | 8 | 23 | 325 | 16 | 309 | 4.52e-34 | 127 |
| MS.gene20413.t1 | AT3G48700 | 31.737 | 334 | 197 | 13 | 12 | 325 | 3 | 325 | 7.89e-34 | 127 |
| MS.gene20413.t1 | AT5G27320 | 34.855 | 241 | 131 | 8 | 22 | 246 | 32 | 262 | 1.24e-33 | 127 |
| MS.gene20413.t1 | AT3G05120 | 33.197 | 244 | 133 | 8 | 22 | 246 | 32 | 264 | 3.18e-29 | 115 |
| MS.gene20413.t1 | AT3G27320 | 33.816 | 207 | 96 | 4 | 68 | 235 | 120 | 324 | 4.14e-29 | 116 |
| MS.gene20413.t1 | AT3G27320 | 33.816 | 207 | 96 | 4 | 68 | 235 | 152 | 356 | 5.41e-29 | 116 |
| MS.gene20413.t1 | AT1G49650 | 34.759 | 187 | 110 | 5 | 28 | 207 | 74 | 255 | 1.33e-28 | 114 |
| MS.gene20413.t1 | AT1G49660 | 34.759 | 187 | 111 | 5 | 28 | 207 | 18 | 200 | 2.61e-28 | 112 |
| MS.gene20413.t1 | AT5G14310 | 33.816 | 207 | 99 | 2 | 78 | 246 | 147 | 353 | 8.63e-28 | 112 |
| MS.gene20413.t1 | AT2G03550 | 33.702 | 181 | 109 | 4 | 31 | 206 | 21 | 195 | 1.36e-24 | 102 |
Find 76 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATGCTATGGAAGCTTTATTT+TGG | 0.193678 | 6.4:-39644767 | MS.gene20413:CDS |
| AAATTGATTGAGTTAATTAA+AGG | 0.231750 | 6.4:-39629865 | MS.gene20413:CDS |
| TCATGATTTCTGTGTGAAAA+TGG | 0.275744 | 6.4:-39644868 | MS.gene20413:CDS |
| CTCAGGCTTCAACTCGGATT+TGG | 0.289643 | 6.4:+39630185 | None:intergenic |
| ATCAATTTCTTAGCTTTCAA+TGG | 0.324723 | 6.4:+39629880 | None:intergenic |
| ACAGGTAGTTTGTGATCATT+AGG | 0.338370 | 6.4:+39644948 | None:intergenic |
| TTATTCTAAGTGCTATCTAA+TGG | 0.341746 | 6.4:-39644703 | MS.gene20413:CDS |
| TTGGTTGATTGTGATGTCTT+TGG | 0.342407 | 6.4:+39645016 | None:intergenic |
| ATATTGCGCCAACCATTCTT+TGG | 0.350196 | 6.4:-39630222 | MS.gene20413:CDS |
| CTTGAGCCACTTAAGATTCA+AGG | 0.397271 | 6.4:-39630249 | MS.gene20413:CDS |
| ATTAAATCAGTAACACATAA+TGG | 0.402226 | 6.4:+39630147 | None:intergenic |
| AGTTTGTGATCATTAGGAAT+TGG | 0.416386 | 6.4:+39644954 | None:intergenic |
| TTGAGAAGTTGGACAAGATA+AGG | 0.416423 | 6.4:-39630053 | MS.gene20413:CDS |
| TATTCTAAGTGCTATCTAAT+GGG | 0.420298 | 6.4:-39644702 | MS.gene20413:CDS |
| GGAAGTGTTGGGTCAGATGA+AGG | 0.423882 | 6.4:+39645053 | None:intergenic |
| GAGATGGTGGTGAAGTTTGA+TGG | 0.432548 | 6.4:+39645162 | None:intergenic |
| TGCTGCAGGTAAACGGTGCT+CGG | 0.433036 | 6.4:+39644797 | None:intergenic |
| ATTAACCCTTGAATCTTAAG+TGG | 0.436258 | 6.4:+39630243 | None:intergenic |
| CATCATATGCTGCAGGTAAA+CGG | 0.447464 | 6.4:+39644790 | None:intergenic |
| GGAAGGATTTGATCATTCTC+AGG | 0.449564 | 6.4:+39630168 | None:intergenic |
| TGATCACGGTCTGCACCAAT+TGG | 0.451844 | 6.4:+39630108 | None:intergenic |
| GGCACAAATGATGGAGGAAA+AGG | 0.453590 | 6.4:-39629962 | MS.gene20413:CDS |
| TCATTAGGAATTGGTTTGTT+GGG | 0.454181 | 6.4:+39644963 | None:intergenic |
| TAAACGAGTGATTGTGTTGT+TGG | 0.456601 | 6.4:+39645097 | None:intergenic |
| ATCATTAGGAATTGGTTTGT+TGG | 0.457225 | 6.4:+39644962 | None:intergenic |
| TAGGAATTGGTTTGTTGGGT+AGG | 0.457808 | 6.4:+39644967 | None:intergenic |
| TGAGAAGTTGGACAAGATAA+GGG | 0.464535 | 6.4:-39630052 | MS.gene20413:CDS |
| GAAGTGTTGGGTCAGATGAA+GGG | 0.468099 | 6.4:+39645054 | None:intergenic |
| GTGAAAGACTTTGATGAAGA+TGG | 0.468586 | 6.4:-39629928 | MS.gene20413:CDS |
| GTGAAAATGGTTGATGAGAT+TGG | 0.471022 | 6.4:-39644855 | MS.gene20413:CDS |
| AGGAGATTAAGATGTTTGTA+AGG | 0.475110 | 6.4:+39645128 | None:intergenic |
| GAAAGAGTTGGCACAAATGA+TGG | 0.476671 | 6.4:-39629971 | MS.gene20413:CDS |
| GCTACCATTGCTTACCATGC+AGG | 0.480177 | 6.4:-39644666 | MS.gene20413:intron |
| TCTTTGGTGAGAACTGTGAT+TGG | 0.488773 | 6.4:+39645032 | None:intergenic |
| AAAGTGTTGGTGAGTGGAAA+TGG | 0.494200 | 6.4:-39630018 | MS.gene20413:CDS |
| TCTTTCCCACGATCAACTAC+TGG | 0.497721 | 6.4:+39629988 | None:intergenic |
| GGATTCATCTTAGCAAGTGC+TGG | 0.508383 | 6.4:-39644903 | MS.gene20413:CDS |
| ACAAGATAAGGGACCTTGGA+TGG | 0.513521 | 6.4:-39630041 | MS.gene20413:CDS |
| TGGGAGTTAGCACTTCCAAT+TGG | 0.514927 | 6.4:-39630123 | MS.gene20413:CDS |
| TTGCGCCAACCATTCTTTGG+TGG | 0.516163 | 6.4:-39630219 | MS.gene20413:CDS |
| AGAACTGTGATTGGAAGTGT+TGG | 0.517776 | 6.4:+39645041 | None:intergenic |
| AAGTGTTGGGTCAGATGAAG+GGG | 0.519565 | 6.4:+39645055 | None:intergenic |
| CACACAGAAATCATGAAACA+TGG | 0.523968 | 6.4:+39644875 | None:intergenic |
| GCTGCAGGTAAACGGTGCTC+GGG | 0.534675 | 6.4:+39644798 | None:intergenic |
| TCAACCAAACAAACAAAACA+TGG | 0.538818 | 6.4:-39645001 | MS.gene20413:CDS |
| TTGAGCCACTTAAGATTCAA+GGG | 0.548071 | 6.4:-39630248 | MS.gene20413:CDS |
| TGTTGGTGAGTGGAAATGGT+GGG | 0.564497 | 6.4:-39630014 | MS.gene20413:CDS |
| ATAGCATCATCATATGCTGC+AGG | 0.567042 | 6.4:+39644783 | None:intergenic |
| TGCAGCATATGATGATGCTA+TGG | 0.567312 | 6.4:-39644781 | MS.gene20413:CDS |
| ATCATTCTCAGGCTTCAACT+CGG | 0.572263 | 6.4:+39630179 | None:intergenic |
| GTGTTGGTGAGTGGAAATGG+TGG | 0.575500 | 6.4:-39630015 | MS.gene20413:CDS |
| TTGGACAAGATAAGGGACCT+TGG | 0.576704 | 6.4:-39630045 | MS.gene20413:CDS |
| GCACAAATGATGGAGGAAAA+GGG | 0.579992 | 6.4:-39629961 | MS.gene20413:CDS |
| TAAGGATCAATGATTTGAGA+TGG | 0.583455 | 6.4:+39645146 | None:intergenic |
| TTGTGTTGTTGGAGTTTCGA+AGG | 0.585794 | 6.4:+39645108 | None:intergenic |
| AATCAGTAACACATAATGGA+AGG | 0.585936 | 6.4:+39630151 | None:intergenic |
| TATGTGTTACTGATTTAATG+TGG | 0.587589 | 6.4:-39630143 | MS.gene20413:CDS |
| CCATGAAAGAAAACAATGAC+AGG | 0.589515 | 6.4:+39644930 | None:intergenic |
| AGTTGATCGTGGGAAAGAGT+TGG | 0.593188 | 6.4:-39629983 | MS.gene20413:CDS |
| ATGTGTTACTGATTTAATGT+GGG | 0.601314 | 6.4:-39630142 | MS.gene20413:CDS |
| TCACCAACACTTTCCATCCA+AGG | 0.607784 | 6.4:+39630028 | None:intergenic |
| GTTGGTGAGTGGAAATGGTG+GGG | 0.613123 | 6.4:-39630013 | MS.gene20413:CDS |
| AGTGTTGGGTCAGATGAAGG+GGG | 0.615911 | 6.4:+39645056 | None:intergenic |
| GGGGATCCAGTAGTTGATCG+TGG | 0.624096 | 6.4:-39629994 | MS.gene20413:CDS |
| GGGATCCAGTAGTTGATCGT+GGG | 0.625780 | 6.4:-39629993 | MS.gene20413:CDS |
| ACGAGCAGTAGAAGTTGTGA+AGG | 0.625964 | 6.4:-39630274 | MS.gene20413:CDS |
| GGATCAATGATTTGAGATGG+TGG | 0.629203 | 6.4:+39645149 | None:intergenic |
| CTAACCTGCATGGTAAGCAA+TGG | 0.629972 | 6.4:+39644662 | None:intergenic |
| GAACTGTGATTGGAAGTGTT+GGG | 0.631835 | 6.4:+39645042 | None:intergenic |
| TATTGCAAAACTCATGATCA+CGG | 0.633224 | 6.4:+39630094 | None:intergenic |
| AACGGTGCTCGGGTGCAAGA+CGG | 0.634589 | 6.4:+39644808 | None:intergenic |
| CAACCAAACAAACAAAACAT+GGG | 0.639568 | 6.4:-39645000 | MS.gene20413:CDS |
| GGATGGAAAGTGTTGGTGAG+TGG | 0.656579 | 6.4:-39630024 | MS.gene20413:CDS |
| AGAGTTGGCACAAATGATGG+AGG | 0.682256 | 6.4:-39629968 | MS.gene20413:CDS |
| TAAGGAATAGTGAAGATGAG+TGG | 0.687511 | 6.4:-39644743 | MS.gene20413:CDS |
| GGACCTTGGATGGAAAGTGT+TGG | 0.707859 | 6.4:-39630031 | MS.gene20413:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.4 | gene | 39629852 | 39645198 | 39629852 | ID=MS.gene20413 |
| chr6.4 | mRNA | 39629852 | 39645198 | 39629852 | ID=MS.gene20413.t1;Parent=MS.gene20413 |
| chr6.4 | exon | 39644667 | 39645198 | 39644667 | ID=MS.gene20413.t1.exon1;Parent=MS.gene20413.t1 |
| chr6.4 | CDS | 39644667 | 39645198 | 39644667 | ID=cds.MS.gene20413.t1;Parent=MS.gene20413.t1 |
| chr6.4 | exon | 39629852 | 39630300 | 39629852 | ID=MS.gene20413.t1.exon2;Parent=MS.gene20413.t1 |
| chr6.4 | CDS | 39629852 | 39630300 | 39629852 | ID=cds.MS.gene20413.t1;Parent=MS.gene20413.t1 |
| Gene Sequence |
| Protein sequence |
