AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene23638

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene23638.t1 MTR_8g035500 96.238 319 12 0 1 319 1 319 0.0 650
MS.gene23638.t1 MTR_8g035540 40.881 318 182 4 5 318 2 317 3.42e-90 272
MS.gene23638.t1 MTR_8g035520 42.138 318 178 4 2 317 11 324 6.85e-90 271
MS.gene23638.t1 MTR_6g464510 39.498 319 179 5 5 317 16 326 5.30e-83 254
MS.gene23638.t1 MTR_6g464280 36.250 320 190 5 5 317 12 324 3.83e-73 229
MS.gene23638.t1 MTR_6g464320 35.312 320 193 6 5 317 13 325 7.15e-72 226
MS.gene23638.t1 MTR_1g102170 37.975 316 181 5 2 312 28 333 2.75e-71 224
MS.gene23638.t1 MTR_1g102160 37.037 324 187 6 4 318 11 326 3.00e-71 224
MS.gene23638.t1 MTR_6g464120 37.821 312 182 6 5 312 17 320 7.31e-68 221
MS.gene23638.t1 MTR_0007s0520 34.936 312 183 8 15 317 24 324 3.87e-63 203
MS.gene23638.t1 MTR_7g406880 32.407 324 198 8 12 317 29 349 1.78e-52 176
MS.gene23638.t1 MTR_6g065110 35.206 267 154 6 29 287 48 303 2.57e-46 160
MS.gene23638.t1 MTR_1g111280 31.818 308 188 10 12 309 34 329 8.83e-45 156
MS.gene23638.t1 MTR_6g065270 32.414 290 176 5 10 287 23 304 5.45e-44 154
MS.gene23638.t1 MTR_6g065120 33.333 282 167 7 17 287 33 304 4.74e-43 151
MS.gene23638.t1 MTR_6g065400 31.633 294 183 6 32 317 50 333 1.16e-41 147
MS.gene23638.t1 MTR_6g065370 30.952 294 185 6 32 317 50 333 1.81e-41 147
MS.gene23638.t1 MTR_7g107040 31.447 318 192 11 12 318 15 317 3.14e-41 146
MS.gene23638.t1 MTR_6g065430 33.213 277 168 7 17 287 31 296 1.04e-38 140
MS.gene23638.t1 MTR_6g065340 30.351 313 179 9 17 317 43 328 6.91e-37 135
MS.gene23638.t1 MTR_4g050140 28.214 280 189 5 44 317 42 315 3.98e-36 132
MS.gene23638.t1 MTR_4g086510 31.621 253 163 4 33 283 35 279 4.79e-35 130
MS.gene23638.t1 MTR_1g082210 31.613 310 178 10 8 296 25 321 9.89e-34 127
MS.gene23638.t1 MTR_3g012440 30.627 271 174 5 57 317 74 340 6.88e-33 124
MS.gene23638.t1 MTR_0007s0480 36.471 170 98 4 157 317 2 170 9.09e-33 119
MS.gene23638.t1 MTR_7g056207 31.557 244 155 5 43 283 77 311 1.01e-31 121
MS.gene23638.t1 MTR_4g086480 30.717 293 182 8 33 317 39 318 1.02e-31 121
MS.gene23638.t1 MTR_3g101420 29.893 281 169 9 12 283 15 276 4.54e-31 119
MS.gene23638.t1 MTR_7g093950 28.981 314 187 10 8 296 25 327 5.51e-31 119
MS.gene23638.t1 MTR_4g086470 31.621 253 160 5 33 283 38 279 2.92e-30 117
MS.gene23638.t1 MTR_1g089310 31.164 292 166 10 8 279 25 301 3.35e-30 117
MS.gene23638.t1 MTR_1g089310 31.164 292 166 10 8 279 41 317 4.57e-30 117
MS.gene23638.t1 MTR_5g005920 29.537 281 184 9 17 290 26 299 4.33e-29 114
MS.gene23638.t1 MTR_4g086540 28.383 303 178 8 17 314 31 299 5.74e-28 110
MS.gene23638.t1 MTR_1g012430 34.199 231 128 7 17 241 16 228 3.28e-27 108
MS.gene23638.t1 MTR_1g105020 31.475 305 179 12 17 301 21 315 7.29e-26 105
MS.gene23638.t1 MTR_5g005900 29.545 264 168 8 17 271 22 276 3.53e-25 103
MS.gene23638.t1 MTR_5g005850 29.487 312 192 11 17 314 26 323 2.46e-24 101
MS.gene23638.t1 MTR_8g040860 29.545 308 194 11 17 314 29 323 5.11e-24 100
MS.gene23638.t1 MTR_3g450680 31.579 266 158 10 17 271 29 281 7.66e-24 100
MS.gene23638.t1 MTR_6g022710 28.906 256 145 7 64 284 147 400 9.29e-24 101
MS.gene23638.t1 MTR_7g051020 30.769 273 173 11 17 283 25 287 7.08e-21 91.7
MS.gene23638.t1 MTR_1g104930 30.403 273 171 11 17 283 25 284 6.96e-20 89.0
MS.gene23638.t1 MTR_1g104933 30.081 246 154 8 43 283 46 278 3.37e-17 81.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene23638.t1 AT2G45610 52.698 315 148 1 4 317 9 323 1.11e-123 357
MS.gene23638.t1 AT2G45600 40.123 324 166 8 5 317 9 315 1.82e-81 250
MS.gene23638.t1 AT5G62180 35.404 322 194 6 5 318 9 324 1.72e-68 217
MS.gene23638.t1 AT1G68620 32.567 261 163 6 17 275 36 285 4.62e-38 138
MS.gene23638.t1 AT5G16080 30.142 282 182 8 32 307 54 326 9.55e-34 127
MS.gene23638.t1 AT5G23530 29.965 287 179 8 16 296 34 304 1.56e-33 126
MS.gene23638.t1 AT5G06570 33.080 263 162 8 40 297 46 299 9.44e-33 124
MS.gene23638.t1 AT5G06570 33.080 263 162 8 40 297 46 299 9.44e-33 124
MS.gene23638.t1 AT5G06570 33.080 263 162 8 40 297 61 314 1.19e-32 124
MS.gene23638.t1 AT5G06570 33.080 263 162 8 40 297 61 314 1.19e-32 124
MS.gene23638.t1 AT5G27320 29.032 310 185 10 8 296 25 320 9.33e-31 119
MS.gene23638.t1 AT3G63010 29.470 302 176 10 16 296 37 322 9.19e-30 116
MS.gene23638.t1 AT3G05120 29.470 302 175 10 8 287 25 310 2.84e-28 112
MS.gene23638.t1 AT1G19190 28.947 266 166 7 26 283 25 275 2.81e-27 108
MS.gene23638.t1 AT1G47480 28.676 272 174 7 12 281 16 269 3.61e-26 105
MS.gene23638.t1 AT5G14310 29.688 256 143 5 65 284 142 396 7.45e-26 107
MS.gene23638.t1 AT3G48690 28.929 280 181 7 12 283 13 282 1.27e-25 104
MS.gene23638.t1 AT2G03550 27.891 294 196 6 29 317 28 310 2.38e-24 100
MS.gene23638.t1 AT3G27320 29.658 263 147 7 58 284 118 378 3.18e-24 102
MS.gene23638.t1 AT3G27320 29.658 263 147 7 58 284 150 410 4.75e-24 102
MS.gene23638.t1 AT1G49640 31.868 182 117 3 22 202 21 196 1.47e-23 99.0
MS.gene23638.t1 AT1G49650 32.979 188 116 4 17 201 74 254 7.03e-23 97.8
MS.gene23638.t1 AT1G49660 27.407 270 183 6 17 282 18 278 1.06e-22 96.7
MS.gene23638.t1 AT3G48700 27.941 204 138 4 33 230 32 232 2.58e-21 92.8

Find 106 sgRNAs with CRISPR-Local

Find 148 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGGAAAATGTCAAGAAATT+AGG 0.125161 8.1:-67543023 MS.gene23638:CDS
CCATCGAGGTGTTATCTTTA+TGG 0.162050 8.1:-67543883 MS.gene23638:CDS
ATCATCAATTGTTATGTCTT+TGG 0.205110 8.1:+67544221 None:intergenic
CTCAATCCAAAGTGGTTATA+TGG 0.248180 8.1:+67544333 None:intergenic
TTAGGGAGGTGTCTTGTTAT+TGG 0.287339 8.1:-67543005 MS.gene23638:CDS
TTCGGGGTTCGCGTCGGTTG+AGG 0.309991 8.1:+67544269 None:intergenic
TGGAAAATGTCAAGAAATTA+GGG 0.314810 8.1:-67543022 MS.gene23638:CDS
AAAGGACGGCCTCGGAAATA+AGG 0.329749 8.1:-67543744 MS.gene23638:intron
TAGGGAGGTGTCTTGTTATT+GGG 0.337672 8.1:-67543004 MS.gene23638:CDS
AATGGCAAAAGGACCACATT+TGG 0.377354 8.1:-67543042 MS.gene23638:CDS
GTCGGTTGAGGGTGTCTTGT+AGG 0.384926 8.1:+67544281 None:intergenic
CGCGAACCCCGAACCTTCAC+CGG 0.388207 8.1:-67544259 MS.gene23638:CDS
ATATTGATTTACTTTCATAA+TGG 0.392883 8.1:-67544123 MS.gene23638:CDS
ATATGGACTTAGAGCCTCTA+AGG 0.394329 8.1:-67543810 MS.gene23638:CDS
TCGGGGTTCGCGTCGGTTGA+GGG 0.394609 8.1:+67544270 None:intergenic
GGCCATTCAGATTGGAGATA+TGG 0.409202 8.1:-67543827 MS.gene23638:CDS
TGGGCCATGGCTTTGCCTAC+GGG 0.409884 8.1:-67543095 MS.gene23638:CDS
ACAAGGCCACTAATCCTTAG+AGG 0.416771 8.1:+67543796 None:intergenic
GACTTTGCCCTTACCAGTGT+TGG 0.417668 8.1:-67543126 MS.gene23638:CDS
TTCAATACGGCCATTCAGAT+TGG 0.423842 8.1:-67543835 MS.gene23638:CDS
TTACTTTCATAATGGTGGTT+GGG 0.428229 8.1:-67544115 MS.gene23638:CDS
GTGGGCCATGGCTTTGCCTA+CGG 0.428532 8.1:-67543096 MS.gene23638:CDS
ATAACCGCACTAGCTCTAGT+AGG 0.431582 8.1:+67542861 None:intergenic
TTTACTTTCATAATGGTGGT+TGG 0.431824 8.1:-67544116 MS.gene23638:CDS
TTTGATCCATATAACCACTT+TGG 0.433703 8.1:-67544339 MS.gene23638:CDS
AGGTGTCTTGTTATTGGGTA+TGG 0.434253 8.1:-67542999 MS.gene23638:CDS
TATCTTTATGGATGTGGTTG+TGG 0.434607 8.1:-67543871 MS.gene23638:CDS
TGTCTCTGTCGCCTTTCGTT+GGG 0.438448 8.1:-67544019 MS.gene23638:CDS
CTCAACCATTGCTCTCCATC+AGG 0.438600 8.1:+67543919 None:intergenic
TTATGAAAGTAAATCAATAT+CGG 0.442305 8.1:+67544126 None:intergenic
TCGAGCATCTTGATATTGTC+CGG 0.444661 8.1:+67543978 None:intergenic
ATCCTAAGCCCGGCTGATGC+TGG 0.450512 8.1:-67544090 MS.gene23638:CDS
TTGTCTCTGTCGCCTTTCGT+TGG 0.451494 8.1:-67544020 MS.gene23638:CDS
CCATTGGATTGCAATACCTA+TGG 0.454707 8.1:+67543060 None:intergenic
TGAAGCTAGGTTTGATCCTG+TGG 0.468258 8.1:-67542910 MS.gene23638:CDS
TTAGAGCCTCTAAGGATTAG+TGG 0.468499 8.1:-67543802 MS.gene23638:CDS
GCCACGGTATTGTCGTTTGA+TGG 0.470251 8.1:+67544156 None:intergenic
TTATGGGTACCAGCATCAGC+CGG 0.476238 8.1:+67544081 None:intergenic
GGGGTTCCACAATATTGATA+TGG 0.477353 8.1:-67542889 MS.gene23638:CDS
TACCAGCATCAGCCGGGCTT+AGG 0.478525 8.1:+67544088 None:intergenic
GCGACAGAGACAACAATACT+AGG 0.481339 8.1:+67544030 None:intergenic
GAGCAATGGTTGAGAGACTA+TGG 0.482302 8.1:-67543910 MS.gene23638:CDS
GCGAACCCCGAACCTTCACC+GGG 0.488799 8.1:-67544258 MS.gene23638:CDS
CATCATGTCCAACACTGGTA+AGG 0.489057 8.1:+67543118 None:intergenic
AGGTGTTATCTTTATGGATG+TGG 0.490354 8.1:-67543877 MS.gene23638:CDS
GTTGGTCAAATGTGGGGTCC+AGG 0.492083 8.1:-67542934 MS.gene23638:CDS
TGGAGAGAAAAGGACGGCCT+CGG 0.492416 8.1:-67543752 MS.gene23638:CDS
GAAGCTAGGTTTGATCCTGT+GGG 0.500369 8.1:-67542909 MS.gene23638:CDS
TTCGTTGGGCGCCAGAGACT+AGG 0.511728 8.1:-67544005 MS.gene23638:CDS
TTGATTTACTTTCATAATGG+TGG 0.512216 8.1:-67544120 MS.gene23638:CDS
TGTCTTGTTATTGGGTATGG+TGG 0.512697 8.1:-67542996 MS.gene23638:CDS
TGACCATGTTGGTCAAATGT+GGG 0.525547 8.1:-67542941 MS.gene23638:CDS
AATTATGAACCTTATTTCCG+AGG 0.528856 8.1:+67543735 None:intergenic
TATTGCAATCCAATGGCAAA+AGG 0.529808 8.1:-67543053 MS.gene23638:CDS
ACACTGGTAAGGGCAAAGTC+TGG 0.531768 8.1:+67543129 None:intergenic
GTGACCATGTTGGTCAAATG+TGG 0.535257 8.1:-67542942 MS.gene23638:CDS
TTCTTCCAAAAGGTTTGCAA+CGG 0.535946 8.1:-67543153 MS.gene23638:intron
TATGGGTACCAGCATCAGCC+GGG 0.538444 8.1:+67544082 None:intergenic
AATCAAATGACGAATCCTGA+TGG 0.539467 8.1:-67543934 MS.gene23638:CDS
GTCCATATCTCCAATCTGAA+TGG 0.540037 8.1:+67543825 None:intergenic
TGTCCGGGTAACCTAGTCTC+TGG 0.551902 8.1:+67543994 None:intergenic
TTGAGGGTGTCTTGTAGGCA+CGG 0.553474 8.1:+67544286 None:intergenic
TACCAGTGTTGGACATGATG+TGG 0.554021 8.1:-67543115 MS.gene23638:CDS
TGGGTATGGTGGAGACATCA+TGG 0.554451 8.1:-67542985 MS.gene23638:CDS
GTTGGACATGATGTGGGCCA+TGG 0.554890 8.1:-67543108 MS.gene23638:CDS
GGTCAACCATATCAATATTG+TGG 0.557103 8.1:+67542883 None:intergenic
GTGGGGTCCAGGTTGAAGCT+AGG 0.557168 8.1:-67542923 MS.gene23638:CDS
CATAAAGATAACACCTCGAT+GGG 0.558427 8.1:+67543884 None:intergenic
TGCAAACATTGTGTTCAATA+CGG 0.560159 8.1:-67543848 MS.gene23638:CDS
GTGCGGTTATAAATATTGCA+AGG 0.561146 8.1:-67542848 MS.gene23638:CDS
CAATATCGGTAAACGCGCCA+CGG 0.563423 8.1:+67544140 None:intergenic
GGATTGAGTTTCAATCCTGA+CGG 0.564876 8.1:-67544318 MS.gene23638:CDS
TGAAGGTTCGGGGTTCGCGT+CGG 0.566328 8.1:+67544263 None:intergenic
AGTCTCTGGCGCCCAACGAA+AGG 0.566700 8.1:+67544008 None:intergenic
CGAGCATCTTGATATTGTCC+GGG 0.569073 8.1:+67543979 None:intergenic
GCACGGTGGAGAGTTCCGTC+AGG 0.576931 8.1:+67544303 None:intergenic
TAACCGCACTAGCTCTAGTA+GGG 0.577127 8.1:+67542862 None:intergenic
TGGTTGGGTCATCCTAAGCC+CGG 0.577877 8.1:-67544100 MS.gene23638:CDS
ATCATGTCCAACACTGGTAA+GGG 0.578520 8.1:+67543119 None:intergenic
TCAATATTGTGGAACCCCAC+AGG 0.582193 8.1:+67542894 None:intergenic
CCATAAAGATAACACCTCGA+TGG 0.586600 8.1:+67543883 None:intergenic
GCCCACATCATGTCCAACAC+TGG 0.603571 8.1:+67543113 None:intergenic
GCGCCAGAGACTAGGTTACC+CGG 0.604483 8.1:-67543997 MS.gene23638:CDS
TGCGGTTATAAATATTGCAA+GGG 0.608893 8.1:-67542847 MS.gene23638:CDS
GGGCCATGGCTTTGCCTACG+GGG 0.610243 8.1:-67543094 MS.gene23638:CDS
CGGGGACAAATCGTGACCAT+AGG 0.613124 8.1:-67543076 MS.gene23638:CDS
AAGCTAGGTTTGATCCTGTG+GGG 0.615240 8.1:-67542908 MS.gene23638:CDS
CCATAGGTATTGCAATCCAA+TGG 0.617062 8.1:-67543060 MS.gene23638:CDS
ACATAGGTTGATTCATAACA+AGG 0.617116 8.1:+67543779 None:intergenic
GATCAAACCTAGCTTCAACC+TGG 0.619274 8.1:+67542916 None:intergenic
GAACCCCGAACCTTCACCGG+GGG 0.629310 8.1:-67544256 MS.gene23638:CDS
ACCAGTGTTGGACATGATGT+GGG 0.635087 8.1:-67543114 MS.gene23638:CDS
ACAAGAATTTGTGACCATGT+TGG 0.638031 8.1:-67542952 MS.gene23638:CDS
TTGGACACCGTAGAAACCCC+CGG 0.639060 8.1:+67544240 None:intergenic
GATTGAAACTCAATCCAAAG+TGG 0.656440 8.1:+67544325 None:intergenic
TGTCCCCGTAGGCAAAGCCA+TGG 0.658805 8.1:+67543091 None:intergenic
GACCATGTTGGTCAAATGTG+GGG 0.660571 8.1:-67542940 MS.gene23638:CDS
TGACCCTACTAGAGCTAGTG+CGG 0.675155 8.1:-67542865 MS.gene23638:CDS
TGGTCACGATTTGTCCCCGT+AGG 0.679424 8.1:+67543080 None:intergenic
AAAATGTCAAGAAATTAGGG+AGG 0.680447 8.1:-67543019 MS.gene23638:CDS
CGAATCCTGATGGAGAGCAA+TGG 0.687815 8.1:-67543924 MS.gene23638:CDS
GACCCCACATTTGACCAACA+TGG 0.691227 8.1:+67542938 None:intergenic
GAGACTATGGTGACCCATCG+AGG 0.705564 8.1:-67543897 MS.gene23638:CDS
ACCATCAAACGACAATACCG+TGG 0.707154 8.1:-67544157 MS.gene23638:CDS
AGGGTGTCTTGTAGGCACGG+TGG 0.724739 8.1:+67544289 None:intergenic
CGAACCCCGAACCTTCACCG+GGG 0.774672 8.1:-67544257 MS.gene23638:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAAAATTTCAAACATCTATA+AGG + chr8.1:67543862-67543881 None:intergenic 15.0%
!! TTATGAAAGTAAATCAATAT+CGG + chr8.1:67543055-67543074 None:intergenic 15.0%
!!! ATATTGATTTACTTTCATAA+TGG - chr8.1:67543055-67543074 MS.gene23638:CDS 15.0%
!!! ATTTCATTTTAGTTTTATAG+AGG - chr8.1:67543800-67543819 MS.gene23638:CDS 15.0%
!!! AAAATATATCTTGACTTGTT+TGG - chr8.1:67543551-67543570 MS.gene23638:intron 20.0%
!!! AAGATTCGAACATTTTTTAT+GGG + chr8.1:67543116-67543135 None:intergenic 20.0%
!!! TGATGATGAGAAAAAAATAT+GGG - chr8.1:67542973-67542992 MS.gene23638:CDS 20.0%
!!! TTGATGATGAGAAAAAAATA+TGG - chr8.1:67542972-67542991 MS.gene23638:CDS 20.0%
! AATTTCAAACATCTATAAGG+TGG + chr8.1:67543859-67543878 None:intergenic 25.0%
! ATCATCAATTGTTATGTCTT+TGG + chr8.1:67542960-67542979 None:intergenic 25.0%
! CAAAATTACTCATTATCATG+TGG - chr8.1:67543943-67543962 MS.gene23638:CDS 25.0%
! TGGAAAATGTCAAGAAATTA+GGG - chr8.1:67544156-67544175 MS.gene23638:CDS 25.0%
! TTGATTTACTTTCATAATGG+TGG - chr8.1:67543058-67543077 MS.gene23638:CDS 25.0%
! TTGGAAAATGTCAAGAAATT+AGG - chr8.1:67544155-67544174 MS.gene23638:CDS 25.0%
!! TTTCTTTCTTTTCTTCCAAA+AGG - chr8.1:67544015-67544034 MS.gene23638:CDS 25.0%
!!! CAAGATTCGAACATTTTTTA+TGG + chr8.1:67543117-67543136 None:intergenic 25.0%
AAAATGTCAAGAAATTAGGG+AGG - chr8.1:67544159-67544178 MS.gene23638:CDS 30.0%
GAGAATAACACATATCTCTT+TGG - chr8.1:67543521-67543540 MS.gene23638:intron 30.0%
GTCTTGATTCAAAGAGATTA+AGG + chr8.1:67543924-67543943 None:intergenic 30.0%
GTGGTTTCTAATACAATAAG+AGG + chr8.1:67543840-67543859 None:intergenic 30.0%
TGCAAACATTGTGTTCAATA+CGG - chr8.1:67543330-67543349 MS.gene23638:intron 30.0%
TGCGGTTATAAATATTGCAA+GGG - chr8.1:67544331-67544350 MS.gene23638:CDS 30.0%
TTACTTTCATAATGGTGGTT+GGG - chr8.1:67543063-67543082 MS.gene23638:CDS 30.0%
TTGATTCAAAGAGATTAAGG+TGG + chr8.1:67543921-67543940 None:intergenic 30.0%
TTTACTTTCATAATGGTGGT+TGG - chr8.1:67543062-67543081 MS.gene23638:CDS 30.0%
TTTGATCCATATAACCACTT+TGG - chr8.1:67542839-67542858 MS.gene23638:CDS 30.0%
! AAATATGGGTTCGAATTTTC+AGG - chr8.1:67542987-67543006 MS.gene23638:CDS 30.0%
! AATTATGAACCTTATTTCCG+AGG + chr8.1:67543446-67543465 None:intergenic 30.0%
! ATGAATCAACCTATGTTTTC+TGG - chr8.1:67543406-67543425 MS.gene23638:intron 30.0%
! GTAAGTTCCTGCTATAATTT+TGG - chr8.1:67543662-67543681 MS.gene23638:intron 30.0%
! TAAATGATGATGCCTATGTT+TGG + chr8.1:67543714-67543733 None:intergenic 30.0%
! TTCTTGACATTTTCCAAATG+TGG + chr8.1:67544152-67544171 None:intergenic 30.0%
! TTTAAAGCCAAACCAAACAT+TGG - chr8.1:67543878-67543897 MS.gene23638:CDS 30.0%
!! ACATAGGTTGATTCATAACA+AGG + chr8.1:67543402-67543421 None:intergenic 30.0%
AATCAAATGACGAATCCTGA+TGG - chr8.1:67543244-67543263 MS.gene23638:intron 35.0%
ACAAGAATTTGTGACCATGT+TGG - chr8.1:67544226-67544245 MS.gene23638:CDS 35.0%
CATAAAGATAACACCTCGAT+GGG + chr8.1:67543297-67543316 None:intergenic 35.0%
CATCAAGCCAAAATTATAGC+AGG + chr8.1:67543672-67543691 None:intergenic 35.0%
CTCAATCCAAAGTGGTTATA+TGG + chr8.1:67542848-67542867 None:intergenic 35.0%
GAGAAATCAAGAGACAAATG+AGG + chr8.1:67543757-67543776 None:intergenic 35.0%
GATTGAAACTCAATCCAAAG+TGG + chr8.1:67542856-67542875 None:intergenic 35.0%
GGTCAACCATATCAATATTG+TGG + chr8.1:67544298-67544317 None:intergenic 35.0%
GTGCGGTTATAAATATTGCA+AGG - chr8.1:67544330-67544349 MS.gene23638:CDS 35.0%
TATCTTTATGGATGTGGTTG+TGG - chr8.1:67543307-67543326 MS.gene23638:intron 35.0%
TATTGCAATCCAATGGCAAA+AGG - chr8.1:67544125-67544144 MS.gene23638:CDS 35.0%
TGTTGATCCAATGTTTGGTT+TGG + chr8.1:67543888-67543907 None:intergenic 35.0%
TTCTTCCAAAAGGTTTGCAA+CGG - chr8.1:67544025-67544044 MS.gene23638:CDS 35.0%
! AGGTGTTATCTTTATGGATG+TGG - chr8.1:67543301-67543320 MS.gene23638:intron 35.0%
! CTTTTCTCTCCAGAAAACAT+AGG + chr8.1:67543418-67543437 None:intergenic 35.0%
!! AGTCTTGTTGATCCAATGTT+TGG + chr8.1:67543893-67543912 None:intergenic 35.0%
!! CTATGTTTTCTGGAGAGAAA+AGG - chr8.1:67543416-67543435 MS.gene23638:intron 35.0%
!!! ATTTTGGCTTGATGTGGAAA+TGG - chr8.1:67543678-67543697 MS.gene23638:intron 35.0%
!!! GCTATAATTTTGGCTTGATG+TGG - chr8.1:67543672-67543691 MS.gene23638:intron 35.0%
!!! TCGTTTGATGGTAGTTTTGT+TGG + chr8.1:67543013-67543032 None:intergenic 35.0%
AATGGCAAAAGGACCACATT+TGG - chr8.1:67544136-67544155 MS.gene23638:CDS 40.0%
ATATGGACTTAGAGCCTCTA+AGG - chr8.1:67543368-67543387 MS.gene23638:intron 40.0%
ATCATGTCCAACACTGGTAA+GGG + chr8.1:67544062-67544081 None:intergenic 40.0%
CCATAAAGATAACACCTCGA+TGG + chr8.1:67543298-67543317 None:intergenic 40.0%
CCATTGGATTGCAATACCTA+TGG + chr8.1:67544121-67544140 None:intergenic 40.0%
GGATTGAGTTTCAATCCTGA+CGG - chr8.1:67542860-67542879 MS.gene23638:CDS 40.0%
GGGGTTCCACAATATTGATA+TGG - chr8.1:67544289-67544308 MS.gene23638:CDS 40.0%
GTCCATATCTCCAATCTGAA+TGG + chr8.1:67543356-67543375 None:intergenic 40.0%
TCGAGCATCTTGATATTGTC+CGG + chr8.1:67543203-67543222 None:intergenic 40.0%
TGACCATGTTGGTCAAATGT+GGG - chr8.1:67544237-67544256 MS.gene23638:CDS 40.0%
TTCAATACGGCCATTCAGAT+TGG - chr8.1:67543343-67543362 MS.gene23638:intron 40.0%
! AAATGTGGTCCTTTTGCCAT+TGG + chr8.1:67544137-67544156 None:intergenic 40.0%
! ACAAGTTTTCTCGTGTCTCA+AGG - chr8.1:67543586-67543605 MS.gene23638:intron 40.0%
! CCATAGGTATTGCAATCCAA+TGG - chr8.1:67544118-67544137 MS.gene23638:CDS 40.0%
! CCATCGAGGTGTTATCTTTA+TGG - chr8.1:67543295-67543314 MS.gene23638:intron 40.0%
! TTAGAGCCTCTAAGGATTAG+TGG - chr8.1:67543376-67543395 MS.gene23638:intron 40.0%
!! AGGTGTCTTGTTATTGGGTA+TGG - chr8.1:67544179-67544198 MS.gene23638:CDS 40.0%
!! GTTTTCTGGAGAGAAAAGGA+CGG - chr8.1:67543420-67543439 MS.gene23638:intron 40.0%
!! TAGGGAGGTGTCTTGTTATT+GGG - chr8.1:67544174-67544193 MS.gene23638:CDS 40.0%
!! TGTCTTGTTATTGGGTATGG+TGG - chr8.1:67544182-67544201 MS.gene23638:CDS 40.0%
!! TTAGGGAGGTGTCTTGTTAT+TGG - chr8.1:67544173-67544192 MS.gene23638:CDS 40.0%
!!! ATGCTCGAGATGCTATTTTG+TGG - chr8.1:67543215-67543234 MS.gene23638:intron 40.0%
!!! TGCTCGAGATGCTATTTTGT+GGG - chr8.1:67543216-67543235 MS.gene23638:intron 40.0%
AAGCTAGGTTTGATCCTGTG+GGG - chr8.1:67544270-67544289 MS.gene23638:CDS 45.0%
ACAAGGCCACTAATCCTTAG+AGG + chr8.1:67543385-67543404 None:intergenic 45.0%
ACCATCAAACGACAATACCG+TGG - chr8.1:67543021-67543040 MS.gene23638:CDS 45.0%
CATCATGTCCAACACTGGTA+AGG + chr8.1:67544063-67544082 None:intergenic 45.0%
CGAGCATCTTGATATTGTCC+GGG + chr8.1:67543202-67543221 None:intergenic 45.0%
GAAGCTAGGTTTGATCCTGT+GGG - chr8.1:67544269-67544288 MS.gene23638:CDS 45.0%
GACCATGTTGGTCAAATGTG+GGG - chr8.1:67544238-67544257 MS.gene23638:CDS 45.0%
GAGCAATGGTTGAGAGACTA+TGG - chr8.1:67543268-67543287 MS.gene23638:intron 45.0%
GATCAAACCTAGCTTCAACC+TGG + chr8.1:67544265-67544284 None:intergenic 45.0%
GCGACAGAGACAACAATACT+AGG + chr8.1:67543151-67543170 None:intergenic 45.0%
GGCCATTCAGATTGGAGATA+TGG - chr8.1:67543351-67543370 MS.gene23638:intron 45.0%
GTGACCATGTTGGTCAAATG+TGG - chr8.1:67544236-67544255 MS.gene23638:CDS 45.0%
TCAATATTGTGGAACCCCAC+AGG + chr8.1:67544287-67544306 None:intergenic 45.0%
TGAAGCTAGGTTTGATCCTG+TGG - chr8.1:67544268-67544287 MS.gene23638:CDS 45.0%
! ACCAGTGTTGGACATGATGT+GGG - chr8.1:67544064-67544083 MS.gene23638:CDS 45.0%
! TACCAGTGTTGGACATGATG+TGG - chr8.1:67544063-67544082 MS.gene23638:CDS 45.0%
!! ATAACCGCACTAGCTCTAGT+AGG + chr8.1:67544320-67544339 None:intergenic 45.0%
!! TAACCGCACTAGCTCTAGTA+GGG + chr8.1:67544319-67544338 None:intergenic 45.0%
!! TGACTTGTTTGGATGTGCGA+TGG - chr8.1:67543562-67543581 MS.gene23638:intron 45.0%
AAAGGACGGCCTCGGAAATA+AGG - chr8.1:67543434-67543453 MS.gene23638:intron 50.0%
ACACTGGTAAGGGCAAAGTC+TGG + chr8.1:67544052-67544071 None:intergenic 50.0%
CAATATCGGTAAACGCGCCA+CGG + chr8.1:67543041-67543060 None:intergenic 50.0%
CGAATCCTGATGGAGAGCAA+TGG - chr8.1:67543254-67543273 MS.gene23638:intron 50.0%
CTCAACCATTGCTCTCCATC+AGG + chr8.1:67543262-67543281 None:intergenic 50.0%
GACCCCACATTTGACCAACA+TGG + chr8.1:67544243-67544262 None:intergenic 50.0%
GACTTTGCCCTTACCAGTGT+TGG - chr8.1:67544052-67544071 MS.gene23638:CDS 50.0%
GGCACTGCACAACCAAACAT+AGG - chr8.1:67543699-67543718 MS.gene23638:intron 50.0%
TGACCCTACTAGAGCTAGTG+CGG - chr8.1:67544313-67544332 MS.gene23638:CDS 50.0%
TGTCTCTGTCGCCTTTCGTT+GGG - chr8.1:67543159-67543178 MS.gene23638:intron 50.0%
TTATGGGTACCAGCATCAGC+CGG + chr8.1:67543100-67543119 None:intergenic 50.0%
TTGTCTCTGTCGCCTTTCGT+TGG - chr8.1:67543158-67543177 MS.gene23638:intron 50.0%
! CTGGTCCGTTGCAAACCTTT+TGG + chr8.1:67544033-67544052 None:intergenic 50.0%
! GCCACGGTATTGTCGTTTGA+TGG + chr8.1:67543025-67543044 None:intergenic 50.0%
! TGGGTATGGTGGAGACATCA+TGG - chr8.1:67544193-67544212 MS.gene23638:CDS 50.0%
!! TTGAGGGTGTCTTGTAGGCA+CGG + chr8.1:67542895-67542914 None:intergenic 50.0%
ACCTTCACCGGGGGTTTCTA+CGG - chr8.1:67542931-67542950 MS.gene23638:CDS 55.0%
AGAAACCCCCGGTGAAGGTT+CGG + chr8.1:67542930-67542949 None:intergenic 55.0%
CGGGGACAAATCGTGACCAT+AGG - chr8.1:67544102-67544121 MS.gene23638:CDS 55.0%
GAGACTATGGTGACCCATCG+AGG - chr8.1:67543281-67543300 MS.gene23638:intron 55.0%
GCCCACATCATGTCCAACAC+TGG + chr8.1:67544068-67544087 None:intergenic 55.0%
GTTGGACATGATGTGGGCCA+TGG - chr8.1:67544070-67544089 MS.gene23638:CDS 55.0%
GTTGGTCAAATGTGGGGTCC+AGG - chr8.1:67544244-67544263 MS.gene23638:CDS 55.0%
TATGGGTACCAGCATCAGCC+GGG + chr8.1:67543099-67543118 None:intergenic 55.0%
TGGAGAGAAAAGGACGGCCT+CGG - chr8.1:67543426-67543445 MS.gene23638:intron 55.0%
TGGTCACGATTTGTCCCCGT+AGG + chr8.1:67544101-67544120 None:intergenic 55.0%
TGGTTGGGTCATCCTAAGCC+CGG - chr8.1:67543078-67543097 MS.gene23638:CDS 55.0%
TGTCCGGGTAACCTAGTCTC+TGG + chr8.1:67543187-67543206 None:intergenic 55.0%
TTGGACACCGTAGAAACCCC+CGG + chr8.1:67542941-67542960 None:intergenic 55.0%
!! GTCGGTTGAGGGTGTCTTGT+AGG + chr8.1:67542900-67542919 None:intergenic 55.0%
AAACCCCCGGTGAAGGTTCG+GGG + chr8.1:67542928-67542947 None:intergenic 60.0%
ACCGTAGAAACCCCCGGTGA+AGG + chr8.1:67542935-67542954 None:intergenic 60.0%
AGTCTCTGGCGCCCAACGAA+AGG + chr8.1:67543173-67543192 None:intergenic 60.0%
ATCCTAAGCCCGGCTGATGC+TGG - chr8.1:67543088-67543107 MS.gene23638:CDS 60.0%
GAAACCCCCGGTGAAGGTTC+GGG + chr8.1:67542929-67542948 None:intergenic 60.0%
GCGCCAGAGACTAGGTTACC+CGG - chr8.1:67543181-67543200 MS.gene23638:intron 60.0%
TACCAGCATCAGCCGGGCTT+AGG + chr8.1:67543093-67543112 None:intergenic 60.0%
TGAAGGTTCGGGGTTCGCGT+CGG + chr8.1:67542918-67542937 None:intergenic 60.0%
TGTCCCCGTAGGCAAAGCCA+TGG + chr8.1:67544090-67544109 None:intergenic 60.0%
TTCGTTGGGCGCCAGAGACT+AGG - chr8.1:67543173-67543192 MS.gene23638:intron 60.0%
! AGGGTGTCTTGTAGGCACGG+TGG + chr8.1:67542892-67542911 None:intergenic 60.0%
! GTGGGCCATGGCTTTGCCTA+CGG - chr8.1:67544082-67544101 MS.gene23638:CDS 60.0%
! GTGGGGTCCAGGTTGAAGCT+AGG - chr8.1:67544255-67544274 MS.gene23638:CDS 60.0%
! TGGGCCATGGCTTTGCCTAC+GGG - chr8.1:67544083-67544102 MS.gene23638:CDS 60.0%
CGAACCCCGAACCTTCACCG+GGG - chr8.1:67542921-67542940 MS.gene23638:CDS 65.0%
CGCGAACCCCGAACCTTCAC+CGG - chr8.1:67542919-67542938 MS.gene23638:CDS 65.0%
GAACCCCGAACCTTCACCGG+GGG - chr8.1:67542922-67542941 MS.gene23638:CDS 65.0%
GCACGGTGGAGAGTTCCGTC+AGG + chr8.1:67542878-67542897 None:intergenic 65.0%
GCGAACCCCGAACCTTCACC+GGG - chr8.1:67542920-67542939 MS.gene23638:CDS 65.0%
! GGGCCATGGCTTTGCCTACG+GGG - chr8.1:67544084-67544103 MS.gene23638:CDS 65.0%
!! TCGGGGTTCGCGTCGGTTGA+GGG + chr8.1:67542911-67542930 None:intergenic 65.0%
!! TTCGGGGTTCGCGTCGGTTG+AGG + chr8.1:67542912-67542931 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr8.1 gene 67542830 67544370 67542830 ID=MS.gene23638
chr8.1 mRNA 67542830 67544370 67542830 ID=MS.gene23638.t1;Parent=MS.gene23638
chr8.1 exon 67543745 67544370 67543745 ID=MS.gene23638.t1.exon1;Parent=MS.gene23638.t1
chr8.1 CDS 67543745 67544370 67543745 ID=cds.MS.gene23638.t1;Parent=MS.gene23638.t1
chr8.1 exon 67542830 67543163 67542830 ID=MS.gene23638.t1.exon2;Parent=MS.gene23638.t1
chr8.1 CDS 67542830 67543163 67542830 ID=cds.MS.gene23638.t1;Parent=MS.gene23638.t1
Gene Sequence

>MS.gene23638

ATGTCAAAATTTGATCCATATAACCACTTTGGATTGAGTTTCAATCCTGACGGAACTCTCCACCGTGCCTACAAGACACCCTCAACCGACGCGAACCCCGAACCTTCACCGGGGGTTTCTACGGTGTCCAAAGACATAACAATTGATGATGAGAAAAAAATATGGGTTCGAATTTTCAGGCCAACAAAACTACCATCAAACGACAATACCGTGGCGCGTTTACCGATATTGATTTACTTTCATAATGGTGGTTGGGTCATCCTAAGCCCGGCTGATGCTGGTACCCATAAAAAATGTTCGAATCTTGCTTCTGACATTCCTAGTATTGTTGTCTCTGTCGCCTTTCGTTGGGCGCCAGAGACTAGGTTACCCGGACAATATCAAGATGCTCGAGATGCTATTTTGTGGGTGAAAAATCAAATGACGAATCCTGATGGAGAGCAATGGTTGAGAGACTATGGTGACCCATCGAGGTGTTATCTTTATGGATGTGGTTGTGGTGCAAACATTGTGTTCAATACGGCCATTCAGATTGGAGATATGGACTTAGAGCCTCTAAGGATTAGTGGCCTTGTTATGAATCAACCTATGTTTTCTGGAGAGAAAAGGACGGCCTCGGAAATAAGGTTCATAATTTTATCATTCTCTTAAAAAAAAAAATTGATCAATTATCATTTTCTTAAATAAATTTGAGAATAACACATATCTCTTTGGTTATGAAAAAATATATCTTGACTTGTTTGGATGTGCGATGGAACAAGTTTTCTCGTGTCTCAAGGCAAAAATTGTCATGATTTATAAAAGCTATTATATGTAGCTTCACTGCTAACACGTAAGTTCCTGCTATAATTTTGGCTTGATGTGGAAATGGCACTGCACAACCAAACATAGGCATCATCATTTATTTTTTAAAAAATATTTGCACCTCATTTGTCTCTTGATTTCTCTTGACTATATTCAGTTATGAAGAATTTCATTTTAGTTTTATAGAGGTTTGAAAATTTCTTCCTCTTATTGTATTAGAAACCACCTTATAGATGTTTGAAATTTTAAAGCCAAACCAAACATTGGATCAACAAGACTCTCAACCACCTTAATCTCTTTGAATCAAGACAAAATTACTCATTATCATGTGGTCACGTTATTATTAAATTTTATTTTGCATGAGTCACATTACTTTGTTTTTTTCTTTCTTTTCTTCCAAAAGGTTTGCAACGGACCAGACTTTGCCCTTACCAGTGTTGGACATGATGTGGGCCATGGCTTTGCCTACGGGGACAAATCGTGACCATAGGTATTGCAATCCAATGGCAAAAGGACCACATTTGGAAAATGTCAAGAAATTAGGGAGGTGTCTTGTTATTGGGTATGGTGGAGACATCATGGTTGATCGACAACAAGAATTTGTGACCATGTTGGTCAAATGTGGGGTCCAGGTTGAAGCTAGGTTTGATCCTGTGGGGTTCCACAATATTGATATGGTTGACCCTACTAGAGCTAGTGCGGTTATAAATATTGCAAGGGAGTTTATTATACTTTGA
Protein sequence

>MS.gene23638.t1

MSKFDPYNHFGLSFNPDGTLHRAYKTPSTDANPEPSPGVSTVSKDITIDDEKKIWVRIFRPTKLPSNDNTVARLPILIYFHNGGWVILSPADAGTHKKCSNLASDIPSIVVSVAFRWAPETRLPGQYQDARDAILWVKNQMTNPDGEQWLRDYGDPSRCYLYGCGCGANIVFNTAIQIGDMDLEPLRISGLVMNQPMFSGEKRTASEIRFATDQTLPLPVLDMMWAMALPTGTNRDHRYCNPMAKGPHLENVKKLGRCLVIGYGGDIMVDRQQEFVTMLVKCGVQVEARFDPVGFHNIDMVDPTRASAVINIAREFIIL