AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene37505

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene37505.t1 MTR_8g035500 95.925 319 13 0 1 319 1 319 0.0 646
MS.gene37505.t1 MTR_8g035540 40.566 318 183 4 5 318 2 317 8.25e-90 271
MS.gene37505.t1 MTR_8g035520 41.824 318 179 4 2 317 11 324 2.12e-89 270
MS.gene37505.t1 MTR_6g464510 39.375 320 180 5 4 317 15 326 2.91e-82 252
MS.gene37505.t1 MTR_6g464280 36.250 320 190 5 5 317 12 324 2.74e-72 226
MS.gene37505.t1 MTR_1g102170 37.975 316 181 5 2 312 28 333 4.10e-71 224
MS.gene37505.t1 MTR_6g464320 35.312 320 193 6 5 317 13 325 4.20e-71 224
MS.gene37505.t1 MTR_1g102160 37.037 324 187 6 4 318 11 326 4.77e-70 221
MS.gene37505.t1 MTR_6g464120 37.821 312 182 6 5 312 17 320 4.59e-67 219
MS.gene37505.t1 MTR_0007s0520 34.936 312 183 8 15 317 24 324 1.67e-63 204
MS.gene37505.t1 MTR_7g406880 32.099 324 199 8 12 317 29 349 1.72e-51 174
MS.gene37505.t1 MTR_6g065110 33.835 266 159 5 29 287 48 303 6.42e-45 156
MS.gene37505.t1 MTR_6g065270 32.414 290 176 5 10 287 23 304 4.65e-44 154
MS.gene37505.t1 MTR_1g111280 31.818 308 188 10 12 309 34 329 1.18e-43 153
MS.gene37505.t1 MTR_6g065120 31.786 280 174 6 17 287 33 304 8.34e-42 148
MS.gene37505.t1 MTR_6g065400 29.618 314 204 6 13 317 28 333 1.68e-41 147
MS.gene37505.t1 MTR_6g065370 28.981 314 206 6 13 317 28 333 2.01e-41 147
MS.gene37505.t1 MTR_7g107040 31.447 318 192 11 12 318 15 317 6.71e-41 145
MS.gene37505.t1 MTR_6g065430 33.213 277 168 7 17 287 31 296 5.71e-39 140
MS.gene37505.t1 MTR_6g065340 30.068 296 173 7 31 317 58 328 1.36e-36 134
MS.gene37505.t1 MTR_4g050140 28.070 285 193 5 39 317 37 315 2.00e-36 133
MS.gene37505.t1 MTR_4g086510 31.225 253 164 4 33 283 35 279 3.51e-34 127
MS.gene37505.t1 MTR_1g082210 31.290 310 179 10 8 296 25 321 1.10e-33 127
MS.gene37505.t1 MTR_3g012440 30.627 271 174 5 57 317 74 340 6.46e-33 124
MS.gene37505.t1 MTR_0007s0480 36.471 170 98 4 157 317 2 170 9.89e-33 119
MS.gene37505.t1 MTR_7g056207 31.148 244 156 5 43 283 77 311 4.58e-31 120
MS.gene37505.t1 MTR_4g086480 30.375 293 183 8 33 317 39 318 7.10e-31 119
MS.gene37505.t1 MTR_3g101420 29.537 281 170 8 12 283 15 276 8.89e-31 118
MS.gene37505.t1 MTR_7g093950 28.662 314 188 10 8 296 25 327 1.48e-29 115
MS.gene37505.t1 MTR_4g086470 32.099 243 152 5 43 283 48 279 1.50e-29 115
MS.gene37505.t1 MTR_5g005920 28.929 280 187 8 17 290 26 299 7.61e-29 113
MS.gene37505.t1 MTR_1g089310 30.822 292 167 10 8 279 41 317 1.46e-28 113
MS.gene37505.t1 MTR_1g089310 30.822 292 167 10 8 279 25 301 1.49e-28 113
MS.gene37505.t1 MTR_4g086540 28.383 303 178 8 17 314 31 299 5.07e-28 110
MS.gene37505.t1 MTR_1g012430 34.199 231 128 7 17 241 16 228 4.28e-27 108
MS.gene37505.t1 MTR_5g005900 29.924 264 167 8 17 271 22 276 1.54e-25 104
MS.gene37505.t1 MTR_1g105020 31.148 305 180 12 17 301 21 315 8.89e-25 102
MS.gene37505.t1 MTR_5g005850 28.571 308 200 9 17 314 26 323 5.19e-24 100
MS.gene37505.t1 MTR_6g022710 28.906 256 145 7 64 284 147 400 2.06e-23 100
MS.gene37505.t1 MTR_3g450680 30.566 265 162 9 17 271 29 281 1.24e-22 96.7
MS.gene37505.t1 MTR_8g040860 28.664 307 198 10 17 314 29 323 1.59e-22 96.3
MS.gene37505.t1 MTR_7g051020 31.502 273 171 11 17 283 25 287 2.18e-21 93.2
MS.gene37505.t1 MTR_1g104930 30.769 273 170 11 17 283 25 284 1.39e-20 90.9
MS.gene37505.t1 MTR_1g104933 30.488 246 153 8 43 283 46 278 8.22e-18 82.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene37505.t1 AT2G45610 52.381 315 149 1 4 317 9 323 3.11e-123 356
MS.gene37505.t1 AT2G45600 40.123 324 166 8 5 317 9 315 2.54e-81 250
MS.gene37505.t1 AT5G62180 35.404 322 194 6 5 318 9 324 1.40e-68 217
MS.gene37505.t1 AT1G68620 33.333 261 161 7 17 275 36 285 1.73e-37 136
MS.gene37505.t1 AT5G16080 30.496 282 181 8 32 307 54 326 3.84e-34 128
MS.gene37505.t1 AT5G23530 29.617 287 180 8 16 296 34 304 2.02e-33 125
MS.gene37505.t1 AT5G06570 32.319 263 164 8 40 297 61 314 4.61e-32 122
MS.gene37505.t1 AT5G06570 32.319 263 164 8 40 297 61 314 4.61e-32 122
MS.gene37505.t1 AT5G06570 32.319 263 164 8 40 297 46 299 4.75e-32 122
MS.gene37505.t1 AT5G06570 32.319 263 164 8 40 297 46 299 4.75e-32 122
MS.gene37505.t1 AT5G27320 29.167 312 182 11 8 296 25 320 4.44e-30 117
MS.gene37505.t1 AT3G63010 29.139 302 177 10 16 296 37 322 2.82e-28 112
MS.gene37505.t1 AT3G05120 29.236 301 177 10 8 287 25 310 8.98e-28 110
MS.gene37505.t1 AT1G19190 28.571 266 167 7 26 283 25 275 5.13e-27 108
MS.gene37505.t1 AT3G48690 29.344 259 167 6 33 283 32 282 6.94e-26 105
MS.gene37505.t1 AT1G47480 28.309 272 175 7 12 281 16 269 1.37e-25 104
MS.gene37505.t1 AT5G14310 29.688 256 143 5 65 284 142 396 1.45e-25 106
MS.gene37505.t1 AT2G03550 28.231 294 195 6 29 317 28 310 5.26e-25 102
MS.gene37505.t1 AT3G27320 28.517 263 150 6 58 284 118 378 9.99e-24 100
MS.gene37505.t1 AT3G27320 28.517 263 150 6 58 284 150 410 1.66e-23 100
MS.gene37505.t1 AT1G49640 31.319 182 118 3 22 202 21 196 2.37e-23 98.2
MS.gene37505.t1 AT1G49650 32.979 188 116 4 17 201 74 254 2.37e-23 99.4
MS.gene37505.t1 AT1G49660 27.407 270 183 6 17 282 18 278 5.78e-23 97.4
MS.gene37505.t1 AT3G48700 28.922 204 136 4 33 230 32 232 9.10e-23 97.1

Find 106 sgRNAs with CRISPR-Local

Find 148 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGGAAAATGTCAAGAAATT+AGG 0.125161 8.3:-60255339 MS.gene37505:CDS
CCATCGAGGTGTTATCTTTA+TGG 0.162050 8.3:-60256203 MS.gene37505:CDS
ATCATCAATTGTTATGTCTT+TGG 0.205110 8.3:+60256541 None:intergenic
CTCAATCCAAAGTGGTTATA+TGG 0.248180 8.3:+60256653 None:intergenic
TTAGGGAGGTGTCTTGTTAT+TGG 0.287339 8.3:-60255321 MS.gene37505:CDS
TTCGGGGTTCGCGTCGGTTG+AGG 0.309991 8.3:+60256589 None:intergenic
TGGAAAATGTCAAGAAATTA+GGG 0.314810 8.3:-60255338 MS.gene37505:CDS
AAAGGACGGCCTCGGAAATA+AGG 0.329749 8.3:-60256064 MS.gene37505:intron
TAGGGAGGTGTCTTGTTATT+GGG 0.337672 8.3:-60255320 MS.gene37505:CDS
AATGGCAAAAGGACCACATT+TGG 0.377354 8.3:-60255358 MS.gene37505:CDS
GTCGGTTGAGGGTGTCTTGT+AGG 0.384926 8.3:+60256601 None:intergenic
CGCGAACCCCGAACCTTCAC+CGG 0.388207 8.3:-60256579 MS.gene37505:CDS
ATATTGATTTACTTTCATAA+TGG 0.392883 8.3:-60256443 MS.gene37505:CDS
ATATGGACTTAGAGCCTCTA+AGG 0.394329 8.3:-60256130 MS.gene37505:CDS
TCGGGGTTCGCGTCGGTTGA+GGG 0.394609 8.3:+60256590 None:intergenic
TGGGCCATGGCTTTGCCTAC+GGG 0.409884 8.3:-60255411 MS.gene37505:CDS
GGCCATGCAGATTGGAGATA+TGG 0.411498 8.3:-60256147 MS.gene37505:CDS
ACAAGGCCACTAATCCTTAG+AGG 0.416771 8.3:+60256116 None:intergenic
GACTTTGCCCTTACCAGTGT+TGG 0.417668 8.3:-60255442 MS.gene37505:CDS
TTACTTTCATAATGGTGGTT+GGG 0.428229 8.3:-60256435 MS.gene37505:CDS
GTGGGCCATGGCTTTGCCTA+CGG 0.428532 8.3:-60255412 MS.gene37505:CDS
ATAACCGCACTAGCTCTAGT+AGG 0.431582 8.3:+60255177 None:intergenic
TTTACTTTCATAATGGTGGT+TGG 0.431824 8.3:-60256436 MS.gene37505:CDS
TTTGATCCATATAACCACTT+TGG 0.433703 8.3:-60256659 MS.gene37505:CDS
AGGTGTCTTGTTATTGGGTA+TGG 0.434253 8.3:-60255315 MS.gene37505:CDS
TATCTTTATGGATGTGGTTG+TGG 0.434607 8.3:-60256191 MS.gene37505:CDS
TGTCTCTGTCGCCTTTCGTT+GGG 0.438448 8.3:-60256339 MS.gene37505:CDS
CTCAACCATTGCTCTCCATC+AGG 0.438600 8.3:+60256239 None:intergenic
TTATGAAAGTAAATCAATAT+CGG 0.442305 8.3:+60256446 None:intergenic
TCGAGCATCTTGATATTGTC+CGG 0.444661 8.3:+60256298 None:intergenic
ATCCTAAGCCCGGCTGATGC+TGG 0.450512 8.3:-60256410 MS.gene37505:CDS
TTGTCTCTGTCGCCTTTCGT+TGG 0.451494 8.3:-60256340 MS.gene37505:CDS
CCATTGGATTGCAATACCTA+TGG 0.454707 8.3:+60255376 None:intergenic
TGAAGCTAGGTTTGATCCTG+TGG 0.468258 8.3:-60255226 MS.gene37505:CDS
TTAGAGCCTCTAAGGATTAG+TGG 0.468499 8.3:-60256122 MS.gene37505:CDS
ACCACGGTATTGTCGTTTGA+TGG 0.469032 8.3:+60256476 None:intergenic
TTCAATACGGCCATGCAGAT+TGG 0.469246 8.3:-60256155 MS.gene37505:CDS
TTATGGGTACCAGCATCAGC+CGG 0.476238 8.3:+60256401 None:intergenic
GCGAACCCCGAACCTTCACC+GGG 0.477024 8.3:-60256578 MS.gene37505:CDS
GGGGTTCCACAATATTGATA+TGG 0.477353 8.3:-60255205 MS.gene37505:CDS
TACCAGCATCAGCCGGGCTT+AGG 0.478525 8.3:+60256408 None:intergenic
GCGACAGAGACAACAATACT+AGG 0.481339 8.3:+60256350 None:intergenic
GAGCAATGGTTGAGAGACTA+TGG 0.482302 8.3:-60256230 MS.gene37505:CDS
CATCATGTCCAACACTGGTA+AGG 0.489057 8.3:+60255434 None:intergenic
AGGTGTTATCTTTATGGATG+TGG 0.490354 8.3:-60256197 MS.gene37505:CDS
GTTGGTCAAATGTGGGGTCC+AGG 0.492083 8.3:-60255250 MS.gene37505:CDS
TGGAGAGAAAAGGACGGCCT+CGG 0.492416 8.3:-60256072 MS.gene37505:CDS
GAAGCTAGGTTTGATCCTGT+GGG 0.500369 8.3:-60255225 MS.gene37505:CDS
TTCGTTGGGCGCCAGAGACT+AGG 0.511728 8.3:-60256325 MS.gene37505:CDS
TTGATTTACTTTCATAATGG+TGG 0.512216 8.3:-60256440 MS.gene37505:CDS
TGTCTTGTTATTGGGTATGG+TGG 0.512697 8.3:-60255312 MS.gene37505:CDS
TGACCATGTTGGTCAAATGT+GGG 0.525547 8.3:-60255257 MS.gene37505:CDS
AATTATGAACCTTATTTCCG+AGG 0.528856 8.3:+60256055 None:intergenic
TATTGCAATCCAATGGCAAA+AGG 0.529808 8.3:-60255369 MS.gene37505:CDS
ACACTGGTAAGGGCAAAGTC+TGG 0.531768 8.3:+60255445 None:intergenic
GTGACCATGTTGGTCAAATG+TGG 0.535257 8.3:-60255258 MS.gene37505:CDS
TTCTTCCAAAAGGTTTGCAA+CGG 0.535946 8.3:-60255469 MS.gene37505:intron
TATGGGTACCAGCATCAGCC+GGG 0.538444 8.3:+60256402 None:intergenic
AATCAAATGACGAATCCTGA+TGG 0.539467 8.3:-60256254 MS.gene37505:CDS
TGTCCGGGTAACCTAGTCTC+TGG 0.551902 8.3:+60256314 None:intergenic
TTGAGGGTGTCTTGTAGGCA+CGG 0.553474 8.3:+60256606 None:intergenic
TACCAGTGTTGGACATGATG+TGG 0.554021 8.3:-60255431 MS.gene37505:CDS
TGGGTATGGTGGAGACATCA+TGG 0.554451 8.3:-60255301 MS.gene37505:CDS
GTTGGACATGATGTGGGCCA+TGG 0.554890 8.3:-60255424 MS.gene37505:CDS
GGTCAACCATATCAATATTG+TGG 0.557103 8.3:+60255199 None:intergenic
GTGGGGTCCAGGTTGAAGCT+AGG 0.557168 8.3:-60255239 MS.gene37505:CDS
CATAAAGATAACACCTCGAT+GGG 0.558427 8.3:+60256204 None:intergenic
TGCAAACATTGTGTTCAATA+CGG 0.560159 8.3:-60256168 MS.gene37505:CDS
GTGCGGTTATAAATATTGCA+AGG 0.561146 8.3:-60255164 MS.gene37505:CDS
GGATTGAGTTTCAATCCTGA+CGG 0.564876 8.3:-60256638 MS.gene37505:CDS
TGAAGGTTCGGGGTTCGCGT+CGG 0.566328 8.3:+60256583 None:intergenic
AGTCTCTGGCGCCCAACGAA+AGG 0.566700 8.3:+60256328 None:intergenic
CGAGCATCTTGATATTGTCC+GGG 0.569073 8.3:+60256299 None:intergenic
GCACGGTGGAGAGTTCCGTC+AGG 0.576931 8.3:+60256623 None:intergenic
TAACCGCACTAGCTCTAGTA+GGG 0.577127 8.3:+60255178 None:intergenic
TGGTTGGGTCATCCTAAGCC+CGG 0.577877 8.3:-60256420 MS.gene37505:CDS
ATCATGTCCAACACTGGTAA+GGG 0.578520 8.3:+60255435 None:intergenic
TCAATATTGTGGAACCCCAC+AGG 0.582193 8.3:+60255210 None:intergenic
CCATAAAGATAACACCTCGA+TGG 0.586600 8.3:+60256203 None:intergenic
GCCCACATCATGTCCAACAC+TGG 0.603571 8.3:+60255429 None:intergenic
GCGCCAGAGACTAGGTTACC+CGG 0.604483 8.3:-60256317 MS.gene37505:CDS
GAACCCCGAACCTTCACCGG+GGG 0.607617 8.3:-60256576 MS.gene37505:CDS
TGCGGTTATAAATATTGCAA+GGG 0.608893 8.3:-60255163 MS.gene37505:CDS
GTCCATATCTCCAATCTGCA+TGG 0.609856 8.3:+60256145 None:intergenic
GGGCCATGGCTTTGCCTACG+GGG 0.610243 8.3:-60255410 MS.gene37505:CDS
CGGGGACAAATCGTGACCAT+AGG 0.613124 8.3:-60255392 MS.gene37505:CDS
AAGCTAGGTTTGATCCTGTG+GGG 0.615240 8.3:-60255224 MS.gene37505:CDS
CCATAGGTATTGCAATCCAA+TGG 0.617062 8.3:-60255376 MS.gene37505:CDS
ACATAGGTTGATTCATAACA+AGG 0.617116 8.3:+60256099 None:intergenic
GATCAAACCTAGCTTCAACC+TGG 0.619274 8.3:+60255232 None:intergenic
ACCAGTGTTGGACATGATGT+GGG 0.635087 8.3:-60255430 MS.gene37505:CDS
ACAAGAATTTGTGACCATGT+TGG 0.638031 8.3:-60255268 MS.gene37505:CDS
CAATATCGGTAAACGCACCA+CGG 0.646416 8.3:+60256460 None:intergenic
GATTGAAACTCAATCCAAAG+TGG 0.656440 8.3:+60256645 None:intergenic
TGTCCCCGTAGGCAAAGCCA+TGG 0.658805 8.3:+60255407 None:intergenic
GACCATGTTGGTCAAATGTG+GGG 0.660571 8.3:-60255256 MS.gene37505:CDS
TGACCCTACTAGAGCTAGTG+CGG 0.675155 8.3:-60255181 MS.gene37505:CDS
TGGTCACGATTTGTCCCCGT+AGG 0.679424 8.3:+60255396 None:intergenic
AAAATGTCAAGAAATTAGGG+AGG 0.680447 8.3:-60255335 MS.gene37505:CDS
CGAATCCTGATGGAGAGCAA+TGG 0.687815 8.3:-60256244 MS.gene37505:CDS
GACCCCACATTTGACCAACA+TGG 0.691227 8.3:+60255254 None:intergenic
AACCCCGAACCTTCACCGGG+GGG 0.694765 8.3:-60256575 MS.gene37505:CDS
GAGACTATGGTGACCCATCG+AGG 0.705564 8.3:-60256217 MS.gene37505:CDS
ACCATCAAACGACAATACCG+TGG 0.710095 8.3:-60256477 MS.gene37505:CDS
AGGGTGTCTTGTAGGCACGG+TGG 0.724739 8.3:+60256609 None:intergenic
CGAACCCCGAACCTTCACCG+GGG 0.756583 8.3:-60256577 MS.gene37505:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAAAATTTCAAACATCTATA+AGG + chr8.3:60256182-60256201 None:intergenic 15.0%
!! TTATGAAAGTAAATCAATAT+CGG + chr8.3:60255371-60255390 None:intergenic 15.0%
!!! ATATTGATTTACTTTCATAA+TGG - chr8.3:60255371-60255390 MS.gene37505:CDS 15.0%
!!! ATTTCATTTTAGTTTTATAG+AGG - chr8.3:60256120-60256139 MS.gene37505:CDS 15.0%
!!! AAAATATATCTTGACTTGTT+TGG - chr8.3:60255871-60255890 MS.gene37505:intron 20.0%
!!! AAGATTCGAACATTTTTTAT+GGG + chr8.3:60255432-60255451 None:intergenic 20.0%
!!! TGATGATGAGAAAAAAATAT+GGG - chr8.3:60255289-60255308 MS.gene37505:CDS 20.0%
!!! TTGATGATGAGAAAAAAATA+TGG - chr8.3:60255288-60255307 MS.gene37505:CDS 20.0%
! AATTTCAAACATCTATAAGG+TGG + chr8.3:60256179-60256198 None:intergenic 25.0%
! ATCATCAATTGTTATGTCTT+TGG + chr8.3:60255276-60255295 None:intergenic 25.0%
! CAAAATTACTCATTATCATG+TGG - chr8.3:60256263-60256282 MS.gene37505:CDS 25.0%
! TGGAAAATGTCAAGAAATTA+GGG - chr8.3:60256476-60256495 MS.gene37505:CDS 25.0%
! TTGATTTACTTTCATAATGG+TGG - chr8.3:60255374-60255393 MS.gene37505:CDS 25.0%
! TTGGAAAATGTCAAGAAATT+AGG - chr8.3:60256475-60256494 MS.gene37505:CDS 25.0%
!! TTTCTTTCTTTTCTTCCAAA+AGG - chr8.3:60256335-60256354 MS.gene37505:CDS 25.0%
!!! CAAGATTCGAACATTTTTTA+TGG + chr8.3:60255433-60255452 None:intergenic 25.0%
AAAATGTCAAGAAATTAGGG+AGG - chr8.3:60256479-60256498 MS.gene37505:CDS 30.0%
GAGAATAACACATATCTCTT+TGG - chr8.3:60255841-60255860 MS.gene37505:intron 30.0%
GTCTTGATTCAAAGAGATTA+AGG + chr8.3:60256244-60256263 None:intergenic 30.0%
GTGGTTTCTAATACAATAAG+AGG + chr8.3:60256160-60256179 None:intergenic 30.0%
TGCAAACATTGTGTTCAATA+CGG - chr8.3:60255646-60255665 MS.gene37505:intron 30.0%
TGCGGTTATAAATATTGCAA+GGG - chr8.3:60256651-60256670 MS.gene37505:CDS 30.0%
TTACTTTCATAATGGTGGTT+GGG - chr8.3:60255379-60255398 MS.gene37505:CDS 30.0%
TTGATTCAAAGAGATTAAGG+TGG + chr8.3:60256241-60256260 None:intergenic 30.0%
TTTACTTTCATAATGGTGGT+TGG - chr8.3:60255378-60255397 MS.gene37505:CDS 30.0%
TTTGATCCATATAACCACTT+TGG - chr8.3:60255155-60255174 MS.gene37505:CDS 30.0%
! AAATATGGGTTCGAATTTTC+AGG - chr8.3:60255303-60255322 MS.gene37505:CDS 30.0%
! AATTATGAACCTTATTTCCG+AGG + chr8.3:60255762-60255781 None:intergenic 30.0%
! ATGAATCAACCTATGTTTTC+TGG - chr8.3:60255722-60255741 MS.gene37505:intron 30.0%
! TAAATGATGATGCCTATGTT+TGG + chr8.3:60256034-60256053 None:intergenic 30.0%
! TTCTTGACATTTTCCAAATG+TGG + chr8.3:60256472-60256491 None:intergenic 30.0%
! TTTAAAGCCAAACCAAACAT+TGG - chr8.3:60256198-60256217 MS.gene37505:CDS 30.0%
!! ACATAGGTTGATTCATAACA+AGG + chr8.3:60255718-60255737 None:intergenic 30.0%
!!! ATTTTGACTTGATGTGGAAA+TGG - chr8.3:60255998-60256017 MS.gene37505:intron 30.0%
AATCAAATGACGAATCCTGA+TGG - chr8.3:60255560-60255579 MS.gene37505:intron 35.0%
ACAAGAATTTGTGACCATGT+TGG - chr8.3:60256546-60256565 MS.gene37505:CDS 35.0%
CATAAAGATAACACCTCGAT+GGG + chr8.3:60255613-60255632 None:intergenic 35.0%
CTCAATCCAAAGTGGTTATA+TGG + chr8.3:60255164-60255183 None:intergenic 35.0%
GAGAAATCAAGAGACAAAAG+AGG + chr8.3:60256077-60256096 None:intergenic 35.0%
GATTGAAACTCAATCCAAAG+TGG + chr8.3:60255172-60255191 None:intergenic 35.0%
GGTCAACCATATCAATATTG+TGG + chr8.3:60256618-60256637 None:intergenic 35.0%
GTGCGGTTATAAATATTGCA+AGG - chr8.3:60256650-60256669 MS.gene37505:CDS 35.0%
TATCTTTATGGATGTGGTTG+TGG - chr8.3:60255623-60255642 MS.gene37505:intron 35.0%
TATTGCAATCCAATGGCAAA+AGG - chr8.3:60256445-60256464 MS.gene37505:CDS 35.0%
TGTTGATCCAATGTTTGGTT+TGG + chr8.3:60256208-60256227 None:intergenic 35.0%
TTCTTCCAAAAGGTTTGCAA+CGG - chr8.3:60256345-60256364 MS.gene37505:CDS 35.0%
! AGGTGTTATCTTTATGGATG+TGG - chr8.3:60255617-60255636 MS.gene37505:intron 35.0%
! CTTTTCTCTCCAGAAAACAT+AGG + chr8.3:60255734-60255753 None:intergenic 35.0%
!! AGTCTTGTTGATCCAATGTT+TGG + chr8.3:60256213-60256232 None:intergenic 35.0%
!! CTATGTTTTCTGGAGAGAAA+AGG - chr8.3:60255732-60255751 MS.gene37505:intron 35.0%
!!! GCTATGATTTTGACTTGATG+TGG - chr8.3:60255992-60256011 MS.gene37505:intron 35.0%
!!! TCGTTTGATGGTAGTTTTGT+TGG + chr8.3:60255329-60255348 None:intergenic 35.0%
AATGGCAAAAGGACCACATT+TGG - chr8.3:60256456-60256475 MS.gene37505:CDS 40.0%
ATATGGACTTAGAGCCTCTA+AGG - chr8.3:60255684-60255703 MS.gene37505:intron 40.0%
ATCATGTCCAACACTGGTAA+GGG + chr8.3:60256382-60256401 None:intergenic 40.0%
CCATAAAGATAACACCTCGA+TGG + chr8.3:60255614-60255633 None:intergenic 40.0%
CCATTGGATTGCAATACCTA+TGG + chr8.3:60256441-60256460 None:intergenic 40.0%
GGATTGAGTTTCAATCCTGA+CGG - chr8.3:60255176-60255195 MS.gene37505:CDS 40.0%
GGGGTTCCACAATATTGATA+TGG - chr8.3:60256609-60256628 MS.gene37505:CDS 40.0%
TCGAGCATCTTGATATTGTC+CGG + chr8.3:60255519-60255538 None:intergenic 40.0%
TGACCATGTTGGTCAAATGT+GGG - chr8.3:60256557-60256576 MS.gene37505:CDS 40.0%
! AAATGTGGTCCTTTTGCCAT+TGG + chr8.3:60256457-60256476 None:intergenic 40.0%
! ACAAGTTTTCTCGTGTCTCA+AGG - chr8.3:60255906-60255925 MS.gene37505:intron 40.0%
! CCATAGGTATTGCAATCCAA+TGG - chr8.3:60256438-60256457 MS.gene37505:CDS 40.0%
! CCATCGAGGTGTTATCTTTA+TGG - chr8.3:60255611-60255630 MS.gene37505:intron 40.0%
! TTAGAGCCTCTAAGGATTAG+TGG - chr8.3:60255692-60255711 MS.gene37505:intron 40.0%
!! AGGTGTCTTGTTATTGGGTA+TGG - chr8.3:60256499-60256518 MS.gene37505:CDS 40.0%
!! GTTTTCTGGAGAGAAAAGGA+CGG - chr8.3:60255736-60255755 MS.gene37505:intron 40.0%
!! TAGGGAGGTGTCTTGTTATT+GGG - chr8.3:60256494-60256513 MS.gene37505:CDS 40.0%
!! TCTTGACTTGTTTGGATGTG+CGG - chr8.3:60255879-60255898 MS.gene37505:intron 40.0%
!! TGTCTTGTTATTGGGTATGG+TGG - chr8.3:60256502-60256521 MS.gene37505:CDS 40.0%
!! TTAGGGAGGTGTCTTGTTAT+TGG - chr8.3:60256493-60256512 MS.gene37505:CDS 40.0%
!!! ATGCTCGAGATGCTATTTTG+TGG - chr8.3:60255531-60255550 MS.gene37505:intron 40.0%
!!! TGCTCGAGATGCTATTTTGT+GGG - chr8.3:60255532-60255551 MS.gene37505:intron 40.0%
AAGCTAGGTTTGATCCTGTG+GGG - chr8.3:60256590-60256609 MS.gene37505:CDS 45.0%
ACAAGGCCACTAATCCTTAG+AGG + chr8.3:60255701-60255720 None:intergenic 45.0%
ACCATCAAACGACAATACCG+TGG - chr8.3:60255337-60255356 MS.gene37505:CDS 45.0%
CAATATCGGTAAACGCACCA+CGG + chr8.3:60255357-60255376 None:intergenic 45.0%
CATCATGTCCAACACTGGTA+AGG + chr8.3:60256383-60256402 None:intergenic 45.0%
CGAGCATCTTGATATTGTCC+GGG + chr8.3:60255518-60255537 None:intergenic 45.0%
GAAGCTAGGTTTGATCCTGT+GGG - chr8.3:60256589-60256608 MS.gene37505:CDS 45.0%
GACCATGTTGGTCAAATGTG+GGG - chr8.3:60256558-60256577 MS.gene37505:CDS 45.0%
GAGCAATGGTTGAGAGACTA+TGG - chr8.3:60255584-60255603 MS.gene37505:intron 45.0%
GATCAAACCTAGCTTCAACC+TGG + chr8.3:60256585-60256604 None:intergenic 45.0%
GCGACAGAGACAACAATACT+AGG + chr8.3:60255467-60255486 None:intergenic 45.0%
GTCCATATCTCCAATCTGCA+TGG + chr8.3:60255672-60255691 None:intergenic 45.0%
GTGACCATGTTGGTCAAATG+TGG - chr8.3:60256556-60256575 MS.gene37505:CDS 45.0%
TCAATATTGTGGAACCCCAC+AGG + chr8.3:60256607-60256626 None:intergenic 45.0%
TGAAGCTAGGTTTGATCCTG+TGG - chr8.3:60256588-60256607 MS.gene37505:CDS 45.0%
TTCAATACGGCCATGCAGAT+TGG - chr8.3:60255659-60255678 MS.gene37505:intron 45.0%
! ACCACGGTATTGTCGTTTGA+TGG + chr8.3:60255341-60255360 None:intergenic 45.0%
! ACCAGTGTTGGACATGATGT+GGG - chr8.3:60256384-60256403 MS.gene37505:CDS 45.0%
! TACCAGTGTTGGACATGATG+TGG - chr8.3:60256383-60256402 MS.gene37505:CDS 45.0%
!! ATAACCGCACTAGCTCTAGT+AGG + chr8.3:60256640-60256659 None:intergenic 45.0%
!! TAACCGCACTAGCTCTAGTA+GGG + chr8.3:60256639-60256658 None:intergenic 45.0%
AAAGGACGGCCTCGGAAATA+AGG - chr8.3:60255750-60255769 MS.gene37505:intron 50.0%
ACACTGGTAAGGGCAAAGTC+TGG + chr8.3:60256372-60256391 None:intergenic 50.0%
CGAATCCTGATGGAGAGCAA+TGG - chr8.3:60255570-60255589 MS.gene37505:intron 50.0%
CTCAACCATTGCTCTCCATC+AGG + chr8.3:60255578-60255597 None:intergenic 50.0%
GACCCCACATTTGACCAACA+TGG + chr8.3:60256563-60256582 None:intergenic 50.0%
GACTTTGCCCTTACCAGTGT+TGG - chr8.3:60256372-60256391 MS.gene37505:CDS 50.0%
GGCACTGCACAACCAAACAT+AGG - chr8.3:60256019-60256038 MS.gene37505:intron 50.0%
GGCCATGCAGATTGGAGATA+TGG - chr8.3:60255667-60255686 MS.gene37505:intron 50.0%
TGACCCTACTAGAGCTAGTG+CGG - chr8.3:60256633-60256652 MS.gene37505:CDS 50.0%
TGTCTCTGTCGCCTTTCGTT+GGG - chr8.3:60255475-60255494 MS.gene37505:intron 50.0%
TTATGGGTACCAGCATCAGC+CGG + chr8.3:60255416-60255435 None:intergenic 50.0%
TTGTCTCTGTCGCCTTTCGT+TGG - chr8.3:60255474-60255493 MS.gene37505:intron 50.0%
! CTGGTCCGTTGCAAACCTTT+TGG + chr8.3:60256353-60256372 None:intergenic 50.0%
! TGGGTATGGTGGAGACATCA+TGG - chr8.3:60256513-60256532 MS.gene37505:CDS 50.0%
!! TGACTTGTTTGGATGTGCGG+TGG - chr8.3:60255882-60255901 MS.gene37505:intron 50.0%
!! TTGAGGGTGTCTTGTAGGCA+CGG + chr8.3:60255211-60255230 None:intergenic 50.0%
CGGGGACAAATCGTGACCAT+AGG - chr8.3:60256422-60256441 MS.gene37505:CDS 55.0%
GAGACTATGGTGACCCATCG+AGG - chr8.3:60255597-60255616 MS.gene37505:intron 55.0%
GCCCACATCATGTCCAACAC+TGG + chr8.3:60256388-60256407 None:intergenic 55.0%
GTTGGACATGATGTGGGCCA+TGG - chr8.3:60256390-60256409 MS.gene37505:CDS 55.0%
GTTGGTCAAATGTGGGGTCC+AGG - chr8.3:60256564-60256583 MS.gene37505:CDS 55.0%
TATGGGTACCAGCATCAGCC+GGG + chr8.3:60255415-60255434 None:intergenic 55.0%
TGGAGAGAAAAGGACGGCCT+CGG - chr8.3:60255742-60255761 MS.gene37505:intron 55.0%
TGGTCACGATTTGTCCCCGT+AGG + chr8.3:60256421-60256440 None:intergenic 55.0%
TGGTTGGGTCATCCTAAGCC+CGG - chr8.3:60255394-60255413 MS.gene37505:CDS 55.0%
TGTCCGGGTAACCTAGTCTC+TGG + chr8.3:60255503-60255522 None:intergenic 55.0%
!! GTCGGTTGAGGGTGTCTTGT+AGG + chr8.3:60255216-60255235 None:intergenic 55.0%
ACCTTCACCGGGGGGTTCTA+CGG - chr8.3:60255247-60255266 MS.gene37505:CDS 60.0%
AGAACCCCCCGGTGAAGGTT+CGG + chr8.3:60255246-60255265 None:intergenic 60.0%
AGTCTCTGGCGCCCAACGAA+AGG + chr8.3:60255489-60255508 None:intergenic 60.0%
ATCCTAAGCCCGGCTGATGC+TGG - chr8.3:60255404-60255423 MS.gene37505:CDS 60.0%
GCGCCAGAGACTAGGTTACC+CGG - chr8.3:60255497-60255516 MS.gene37505:intron 60.0%
TACCAGCATCAGCCGGGCTT+AGG + chr8.3:60255409-60255428 None:intergenic 60.0%
TGAAGGTTCGGGGTTCGCGT+CGG + chr8.3:60255234-60255253 None:intergenic 60.0%
TGTCCCCGTAGGCAAAGCCA+TGG + chr8.3:60256410-60256429 None:intergenic 60.0%
TTCGTTGGGCGCCAGAGACT+AGG - chr8.3:60255489-60255508 MS.gene37505:intron 60.0%
TTGGACACCGTAGAACCCCC+CGG + chr8.3:60255257-60255276 None:intergenic 60.0%
! AGGGTGTCTTGTAGGCACGG+TGG + chr8.3:60255208-60255227 None:intergenic 60.0%
! GTGGGCCATGGCTTTGCCTA+CGG - chr8.3:60256402-60256421 MS.gene37505:CDS 60.0%
! GTGGGGTCCAGGTTGAAGCT+AGG - chr8.3:60256575-60256594 MS.gene37505:CDS 60.0%
! TGGGCCATGGCTTTGCCTAC+GGG - chr8.3:60256403-60256422 MS.gene37505:CDS 60.0%
AACCCCCCGGTGAAGGTTCG+GGG + chr8.3:60255244-60255263 None:intergenic 65.0%
AACCCCGAACCTTCACCGGG+GGG - chr8.3:60255239-60255258 MS.gene37505:CDS 65.0%
ACCGTAGAACCCCCCGGTGA+AGG + chr8.3:60255251-60255270 None:intergenic 65.0%
CGAACCCCGAACCTTCACCG+GGG - chr8.3:60255237-60255256 MS.gene37505:CDS 65.0%
CGCGAACCCCGAACCTTCAC+CGG - chr8.3:60255235-60255254 MS.gene37505:CDS 65.0%
GAACCCCCCGGTGAAGGTTC+GGG + chr8.3:60255245-60255264 None:intergenic 65.0%
GAACCCCGAACCTTCACCGG+GGG - chr8.3:60255238-60255257 MS.gene37505:CDS 65.0%
GCACGGTGGAGAGTTCCGTC+AGG + chr8.3:60255194-60255213 None:intergenic 65.0%
GCGAACCCCGAACCTTCACC+GGG - chr8.3:60255236-60255255 MS.gene37505:CDS 65.0%
! GGGCCATGGCTTTGCCTACG+GGG - chr8.3:60256404-60256423 MS.gene37505:CDS 65.0%
!! TCGGGGTTCGCGTCGGTTGA+GGG + chr8.3:60255227-60255246 None:intergenic 65.0%
!! TTCGGGGTTCGCGTCGGTTG+AGG + chr8.3:60255228-60255247 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr8.3 gene 60255146 60256690 60255146 ID=MS.gene37505
chr8.3 mRNA 60255146 60256690 60255146 ID=MS.gene37505.t1;Parent=MS.gene37505
chr8.3 exon 60256065 60256690 60256065 ID=MS.gene37505.t1.exon1;Parent=MS.gene37505.t1
chr8.3 CDS 60256065 60256690 60256065 ID=cds.MS.gene37505.t1;Parent=MS.gene37505.t1
chr8.3 exon 60255146 60255479 60255146 ID=MS.gene37505.t1.exon2;Parent=MS.gene37505.t1
chr8.3 CDS 60255146 60255479 60255146 ID=cds.MS.gene37505.t1;Parent=MS.gene37505.t1
Gene Sequence

>MS.gene37505

ATGTCAAAATTTGATCCATATAACCACTTTGGATTGAGTTTCAATCCTGACGGAACTCTCCACCGTGCCTACAAGACACCCTCAACCGACGCGAACCCCGAACCTTCACCGGGGGGTTCTACGGTGTCCAAAGACATAACAATTGATGATGAGAAAAAAATATGGGTTCGAATTTTCAGGCCAACAAAACTACCATCAAACGACAATACCGTGGTGCGTTTACCGATATTGATTTACTTTCATAATGGTGGTTGGGTCATCCTAAGCCCGGCTGATGCTGGTACCCATAAAAAATGTTCGAATCTTGCTTCTGACATTCCTAGTATTGTTGTCTCTGTCGCCTTTCGTTGGGCGCCAGAGACTAGGTTACCCGGACAATATCAAGATGCTCGAGATGCTATTTTGTGGGTGAAAAATCAAATGACGAATCCTGATGGAGAGCAATGGTTGAGAGACTATGGTGACCCATCGAGGTGTTATCTTTATGGATGTGGTTGTGGTGCAAACATTGTGTTCAATACGGCCATGCAGATTGGAGATATGGACTTAGAGCCTCTAAGGATTAGTGGCCTTGTTATGAATCAACCTATGTTTTCTGGAGAGAAAAGGACGGCCTCGGAAATAAGGTTCATAATTTTATCATTCTCTTAAAAAAAAAAAAAAATTGATCAATTATCATTCTCTTAAATAAATTTGAGAATAACACATATCTCTTTGGTTATGAAAAAATATATCTTGACTTGTTTGGATGTGCGGTGGAACAAGTTTTCTCGTGTCTCAAGGCAAAAATTGTCATGATTTAAAAAAGCTATTATATGTAGCTTCACTGCTAACACGTAAGTTTCTGCTATGATTTTGACTTGATGTGGAAATGGCACTGCACAACCAAACATAGGCATCATCATTTATTTTTTAAAAAATATTTGCACCTCTTTTGTCTCTTGATTTCTCTTGATTATATTCAGTTATGAAGAATTTCATTTTAGTTTTATAGAGGTTTGAAAATTTCTTCCTCTTATTGTATTAGAAACCACCTTATAGATGTTTGAAATTTTAAAGCCAAACCAAACATTGGATCAACAAGACTCTCAACCACCTTAATCTCTTTGAATCAAGACAAAATTACTCATTATCATGTGGTCACGTTATTATTAAATTTTATTTTGCATGAGTCACATTACTTTGTTTTTTTCTTTCTTTTCTTCCAAAAGGTTTGCAACGGACCAGACTTTGCCCTTACCAGTGTTGGACATGATGTGGGCCATGGCTTTGCCTACGGGGACAAATCGTGACCATAGGTATTGCAATCCAATGGCAAAAGGACCACATTTGGAAAATGTCAAGAAATTAGGGAGGTGTCTTGTTATTGGGTATGGTGGAGACATCATGGTTGATCGACAACAAGAATTTGTGACCATGTTGGTCAAATGTGGGGTCCAGGTTGAAGCTAGGTTTGATCCTGTGGGGTTCCACAATATTGATATGGTTGACCCTACTAGAGCTAGTGCGGTTATAAATATTGCAAGGGAGTTTATTATACTTTGA
Protein sequence

>MS.gene37505.t1

MSKFDPYNHFGLSFNPDGTLHRAYKTPSTDANPEPSPGGSTVSKDITIDDEKKIWVRIFRPTKLPSNDNTVVRLPILIYFHNGGWVILSPADAGTHKKCSNLASDIPSIVVSVAFRWAPETRLPGQYQDARDAILWVKNQMTNPDGEQWLRDYGDPSRCYLYGCGCGANIVFNTAMQIGDMDLEPLRISGLVMNQPMFSGEKRTASEIRFATDQTLPLPVLDMMWAMALPTGTNRDHRYCNPMAKGPHLENVKKLGRCLVIGYGGDIMVDRQQEFVTMLVKCGVQVEARFDPVGFHNIDMVDPTRASAVINIAREFIIL