AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene87835

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene87835.t1 MTR_8g035500 96.238 319 12 0 1 319 1 319 0.0 648
MS.gene87835.t1 MTR_8g035540 40.881 318 182 4 5 318 2 317 2.17e-90 273
MS.gene87835.t1 MTR_8g035520 42.138 318 178 4 2 317 11 324 4.34e-90 272
MS.gene87835.t1 MTR_6g464510 39.375 320 180 5 4 317 15 326 2.55e-82 252
MS.gene87835.t1 MTR_6g464280 36.250 320 190 5 5 317 12 324 1.42e-72 227
MS.gene87835.t1 MTR_6g464320 35.312 320 193 6 5 317 13 325 2.32e-71 224
MS.gene87835.t1 MTR_1g102170 37.855 317 182 5 2 313 28 334 2.93e-71 224
MS.gene87835.t1 MTR_1g102160 37.037 324 187 6 4 318 11 326 2.17e-70 222
MS.gene87835.t1 MTR_6g464120 37.821 312 182 6 5 312 17 320 2.03e-67 219
MS.gene87835.t1 MTR_0007s0520 34.936 312 183 8 15 317 24 324 1.78e-63 204
MS.gene87835.t1 MTR_7g406880 33.910 289 177 5 12 288 29 315 1.13e-51 174
MS.gene87835.t1 MTR_6g065110 34.831 267 155 6 29 287 48 303 6.16e-45 156
MS.gene87835.t1 MTR_6g065270 32.414 290 176 5 10 287 23 304 4.90e-44 154
MS.gene87835.t1 MTR_1g111280 31.818 308 188 10 12 309 34 329 5.90e-44 154
MS.gene87835.t1 MTR_6g065120 31.429 280 175 5 17 287 33 304 2.23e-42 149
MS.gene87835.t1 MTR_6g065400 29.618 314 204 6 13 317 28 333 1.48e-41 147
MS.gene87835.t1 MTR_6g065370 28.981 314 206 6 13 317 28 333 1.79e-41 147
MS.gene87835.t1 MTR_7g107040 31.447 318 192 11 12 318 15 317 6.23e-41 145
MS.gene87835.t1 MTR_6g065430 33.213 277 168 7 17 287 31 296 5.20e-39 140
MS.gene87835.t1 MTR_4g050140 28.070 285 193 5 39 317 37 315 1.11e-36 134
MS.gene87835.t1 MTR_6g065340 30.068 296 173 7 31 317 58 328 1.24e-36 134
MS.gene87835.t1 MTR_4g086510 31.225 253 164 4 33 283 35 279 1.90e-34 128
MS.gene87835.t1 MTR_1g082210 31.290 310 179 10 8 296 25 321 3.32e-33 125
MS.gene87835.t1 MTR_3g012440 30.627 271 174 5 57 317 74 340 6.67e-33 124
MS.gene87835.t1 MTR_0007s0480 36.471 170 98 4 157 317 2 170 9.79e-33 119
MS.gene87835.t1 MTR_7g056207 31.148 244 156 5 43 283 77 311 2.95e-31 120
MS.gene87835.t1 MTR_4g086480 30.375 293 183 8 33 317 39 318 3.82e-31 119
MS.gene87835.t1 MTR_3g101420 29.537 281 170 8 12 283 15 276 5.14e-31 119
MS.gene87835.t1 MTR_4g086470 32.099 243 152 5 43 283 48 279 7.78e-30 115
MS.gene87835.t1 MTR_7g093950 32.340 235 144 6 69 296 101 327 1.16e-29 116
MS.gene87835.t1 MTR_5g005920 28.929 280 187 8 17 290 26 299 5.65e-29 114
MS.gene87835.t1 MTR_1g089310 30.822 292 167 10 8 279 25 301 7.76e-29 114
MS.gene87835.t1 MTR_1g089310 30.822 292 167 10 8 279 41 317 9.54e-29 114
MS.gene87835.t1 MTR_4g086540 28.383 303 178 8 17 314 31 299 4.87e-28 111
MS.gene87835.t1 MTR_1g012430 34.199 231 128 7 17 241 16 228 2.56e-27 108
MS.gene87835.t1 MTR_5g005900 29.924 264 167 8 17 271 22 276 1.49e-25 104
MS.gene87835.t1 MTR_1g105020 31.148 305 180 12 17 301 21 315 5.10e-25 103
MS.gene87835.t1 MTR_5g005850 31.933 238 144 8 17 246 26 253 5.40e-24 100
MS.gene87835.t1 MTR_6g022710 28.906 256 145 7 64 284 147 400 1.71e-23 100
MS.gene87835.t1 MTR_3g450680 30.566 265 162 9 17 271 29 281 5.46e-23 97.4
MS.gene87835.t1 MTR_8g040860 28.664 307 198 10 17 314 29 323 6.52e-23 97.4
MS.gene87835.t1 MTR_7g051020 31.136 273 172 11 17 283 25 287 1.51e-21 93.6
MS.gene87835.t1 MTR_1g104930 30.769 273 170 11 17 283 25 284 1.40e-20 90.9
MS.gene87835.t1 MTR_1g104933 30.488 246 153 8 43 283 46 278 7.83e-18 82.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene87835.t1 AT2G45610 52.698 315 148 1 4 317 9 323 7.04e-124 358
MS.gene87835.t1 AT2G45600 40.123 324 166 8 5 317 9 315 1.48e-81 250
MS.gene87835.t1 AT5G62180 35.404 322 194 6 5 318 9 324 5.66e-69 218
MS.gene87835.t1 AT1G68620 33.333 261 161 7 17 275 36 285 7.18e-38 137
MS.gene87835.t1 AT5G16080 30.142 282 182 8 32 307 54 326 1.10e-33 127
MS.gene87835.t1 AT5G23530 29.617 287 180 8 16 296 34 304 2.08e-33 125
MS.gene87835.t1 AT5G06570 33.080 263 162 8 40 297 46 299 1.89e-32 123
MS.gene87835.t1 AT5G06570 33.080 263 162 8 40 297 46 299 1.89e-32 123
MS.gene87835.t1 AT5G06570 33.080 263 162 8 40 297 61 314 2.08e-32 123
MS.gene87835.t1 AT5G06570 33.080 263 162 8 40 297 61 314 2.08e-32 123
MS.gene87835.t1 AT5G27320 28.710 310 186 10 8 296 25 320 6.83e-30 116
MS.gene87835.t1 AT3G63010 29.139 302 177 10 16 296 37 322 1.77e-28 113
MS.gene87835.t1 AT3G05120 29.139 302 176 10 8 287 25 310 8.62e-28 110
MS.gene87835.t1 AT1G19190 28.571 266 167 7 26 283 25 275 3.24e-27 108
MS.gene87835.t1 AT3G48690 29.730 259 166 6 33 283 32 282 4.62e-26 105
MS.gene87835.t1 AT5G14310 29.688 256 143 5 65 284 142 396 1.14e-25 106
MS.gene87835.t1 AT1G47480 28.309 272 175 7 12 281 16 269 1.33e-25 104
MS.gene87835.t1 AT2G03550 28.231 294 195 6 29 317 28 310 2.98e-25 103
MS.gene87835.t1 AT3G27320 28.517 263 150 6 58 284 118 378 8.30e-24 101
MS.gene87835.t1 AT3G27320 28.517 263 150 6 58 284 150 410 1.08e-23 101
MS.gene87835.t1 AT1G49640 31.319 182 118 3 22 202 21 196 1.35e-23 99.0
MS.gene87835.t1 AT1G49650 32.979 188 116 4 17 201 74 254 2.67e-23 99.0
MS.gene87835.t1 AT1G49660 27.407 270 183 6 17 282 18 278 5.34e-23 97.4
MS.gene87835.t1 AT3G48700 28.431 204 137 4 33 230 32 232 2.42e-22 95.9

Find 106 sgRNAs with CRISPR-Local

Find 149 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGGAAAATGTCAAGAAATT+AGG 0.125161 8.4:-61991788 MS.gene87835:CDS
CCATCGAGGTGTTATCTTTA+TGG 0.162050 8.4:-61992646 MS.gene87835:CDS
ATCATCAATTGTTATGTCTT+TGG 0.205110 8.4:+61992984 None:intergenic
CTCAATCCAAAGTGGTTATA+TGG 0.248180 8.4:+61993096 None:intergenic
TTAGGGAGGTGTCTTGTTAT+TGG 0.287339 8.4:-61991770 MS.gene87835:CDS
TTCGGGGTTCGCGTCGGTTG+AGG 0.309991 8.4:+61993032 None:intergenic
TGGAAAATGTCAAGAAATTA+GGG 0.314810 8.4:-61991787 MS.gene87835:CDS
AAAGGACGGCCTCGGAAATA+AGG 0.329749 8.4:-61992507 MS.gene87835:intron
TAGGGAGGTGTCTTGTTATT+GGG 0.337672 8.4:-61991769 MS.gene87835:CDS
AATGGCAAAAGGACCACATT+TGG 0.377354 8.4:-61991807 MS.gene87835:CDS
GTCGGTTGAGGGTGTCTTGT+AGG 0.384926 8.4:+61993044 None:intergenic
CGCGAACCCCGAACCTTCAC+CGG 0.388207 8.4:-61993022 MS.gene87835:CDS
ATATTGATTTACTTTCATAA+TGG 0.392883 8.4:-61992886 MS.gene87835:CDS
ATATGGACTTAGAGCCTCTA+AGG 0.394329 8.4:-61992573 MS.gene87835:CDS
TCGGGGTTCGCGTCGGTTGA+GGG 0.394609 8.4:+61993033 None:intergenic
TGGGCCATGGCTTTGCCTAC+GGG 0.409884 8.4:-61991860 MS.gene87835:CDS
GGCCATGCAGATTGGAGATA+TGG 0.411498 8.4:-61992590 MS.gene87835:CDS
ACAAGGCCACTAATCCTTAG+AGG 0.416771 8.4:+61992559 None:intergenic
GACTTTGCCCTTACCAGTGT+TGG 0.417668 8.4:-61991891 MS.gene87835:CDS
TTACTTTCATAATGGTGGTT+GGG 0.428229 8.4:-61992878 MS.gene87835:CDS
GTGGGCCATGGCTTTGCCTA+CGG 0.428532 8.4:-61991861 MS.gene87835:CDS
ATAACCGCACTAGCTCTAGT+AGG 0.431582 8.4:+61991626 None:intergenic
TTTACTTTCATAATGGTGGT+TGG 0.431824 8.4:-61992879 MS.gene87835:CDS
TTTGATCCATATAACCACTT+TGG 0.433703 8.4:-61993102 MS.gene87835:CDS
AGGTGTCTTGTTATTGGGTA+TGG 0.434253 8.4:-61991764 MS.gene87835:CDS
TATCTTTATGGATGTGGTTG+TGG 0.434607 8.4:-61992634 MS.gene87835:CDS
TGTCTCTGTCGCCTTTCGTT+GGG 0.438448 8.4:-61992782 MS.gene87835:CDS
CTCAACCATTGCTCTCCATC+AGG 0.438600 8.4:+61992682 None:intergenic
TTATGAAAGTAAATCAATAT+CGG 0.442305 8.4:+61992889 None:intergenic
TCGAGCATCTTGATATTGTC+CGG 0.444661 8.4:+61992741 None:intergenic
ATCCTAAGCCCGGCTGATGC+TGG 0.450512 8.4:-61992853 MS.gene87835:CDS
TTGTCTCTGTCGCCTTTCGT+TGG 0.451494 8.4:-61992783 MS.gene87835:CDS
CCATTGGATTGCAATACCTA+TGG 0.454707 8.4:+61991825 None:intergenic
TGAAGCTAGGTTTGATCCTG+TGG 0.468258 8.4:-61991675 MS.gene87835:CDS
TTAGAGCCTCTAAGGATTAG+TGG 0.468499 8.4:-61992565 MS.gene87835:CDS
TTCAATACGGCCATGCAGAT+TGG 0.469246 8.4:-61992598 MS.gene87835:CDS
GCCACGGTATTGTCGTTTGA+TGG 0.470251 8.4:+61992919 None:intergenic
TTATGGGTACCAGCATCAGC+CGG 0.476238 8.4:+61992844 None:intergenic
GCGAACCCCGAACCTTCACC+GGG 0.477024 8.4:-61993021 MS.gene87835:CDS
GGGGTTCCACAATATTGATA+TGG 0.477353 8.4:-61991654 MS.gene87835:CDS
TACCAGCATCAGCCGGGCTT+AGG 0.478525 8.4:+61992851 None:intergenic
GCGACAGAGACAACAATACT+AGG 0.481339 8.4:+61992793 None:intergenic
GAGCAATGGTTGAGAGACTA+TGG 0.482302 8.4:-61992673 MS.gene87835:CDS
CATCATGTCCAACACTGGTA+AGG 0.489057 8.4:+61991883 None:intergenic
AGGTGTTATCTTTATGGATG+TGG 0.490354 8.4:-61992640 MS.gene87835:CDS
GTTGGTCAAATGTGGGGTCC+AGG 0.492083 8.4:-61991699 MS.gene87835:CDS
TGGAGAGAAAAGGACGGCCT+CGG 0.492416 8.4:-61992515 MS.gene87835:CDS
GAAGCTAGGTTTGATCCTGT+GGG 0.500369 8.4:-61991674 MS.gene87835:CDS
TTCGTTGGGCGCCAGAGACT+AGG 0.511728 8.4:-61992768 MS.gene87835:CDS
TTGATTTACTTTCATAATGG+TGG 0.512216 8.4:-61992883 MS.gene87835:CDS
TGTCTTGTTATTGGGTATGG+TGG 0.512697 8.4:-61991761 MS.gene87835:CDS
TGACCATGTTGGTCAAATGT+GGG 0.525547 8.4:-61991706 MS.gene87835:CDS
AATTATGAACCTTATTTCCG+AGG 0.528856 8.4:+61992498 None:intergenic
TATTGCAATCCAATGGCAAA+AGG 0.529808 8.4:-61991818 MS.gene87835:CDS
ACACTGGTAAGGGCAAAGTC+TGG 0.531768 8.4:+61991894 None:intergenic
GTGACCATGTTGGTCAAATG+TGG 0.535257 8.4:-61991707 MS.gene87835:CDS
TTCTTCCAAAAGGTTTGCAA+CGG 0.535946 8.4:-61991918 MS.gene87835:intron
TATGGGTACCAGCATCAGCC+GGG 0.538444 8.4:+61992845 None:intergenic
AATCAAATGACGAATCCTGA+TGG 0.539467 8.4:-61992697 MS.gene87835:CDS
TGTCCGGGTAACCTAGTCTC+TGG 0.551902 8.4:+61992757 None:intergenic
TTGAGGGTGTCTTGTAGGCA+CGG 0.553474 8.4:+61993049 None:intergenic
TACCAGTGTTGGACATGATG+TGG 0.554021 8.4:-61991880 MS.gene87835:CDS
TGGGTATGGTGGAGACATCA+TGG 0.554451 8.4:-61991750 MS.gene87835:CDS
GTTGGACATGATGTGGGCCA+TGG 0.554890 8.4:-61991873 MS.gene87835:CDS
GGTCAACCATATCAATATTG+TGG 0.557103 8.4:+61991648 None:intergenic
GTGGGGTCCAGGTTGAAGCT+AGG 0.557168 8.4:-61991688 MS.gene87835:CDS
CATAAAGATAACACCTCGAT+GGG 0.558427 8.4:+61992647 None:intergenic
TGCAAACATTGTGTTCAATA+CGG 0.560159 8.4:-61992611 MS.gene87835:CDS
GTGCGGTTATAAATATTGCA+AGG 0.561146 8.4:-61991613 MS.gene87835:CDS
CAATATCGGTAAACGCGCCA+CGG 0.563423 8.4:+61992903 None:intergenic
GGATTGAGTTTCAATCCTGA+CGG 0.564876 8.4:-61993081 MS.gene87835:CDS
TGAAGGTTCGGGGTTCGCGT+CGG 0.566328 8.4:+61993026 None:intergenic
AGTCTCTGGCGCCCAACGAA+AGG 0.566700 8.4:+61992771 None:intergenic
CGAGCATCTTGATATTGTCC+GGG 0.569073 8.4:+61992742 None:intergenic
GCACGGTGGAGAGTTCCGTC+AGG 0.576931 8.4:+61993066 None:intergenic
TAACCGCACTAGCTCTAGTA+GGG 0.577127 8.4:+61991627 None:intergenic
TGGTTGGGTCATCCTAAGCC+CGG 0.577877 8.4:-61992863 MS.gene87835:CDS
ATCATGTCCAACACTGGTAA+GGG 0.578520 8.4:+61991884 None:intergenic
TCAATATTGTGGAACCCCAC+AGG 0.582193 8.4:+61991659 None:intergenic
CCATAAAGATAACACCTCGA+TGG 0.586600 8.4:+61992646 None:intergenic
GCCCACATCATGTCCAACAC+TGG 0.603571 8.4:+61991878 None:intergenic
GCGCCAGAGACTAGGTTACC+CGG 0.604483 8.4:-61992760 MS.gene87835:CDS
GAACCCCGAACCTTCACCGG+GGG 0.607617 8.4:-61993019 MS.gene87835:CDS
TGCGGTTATAAATATTGCAA+GGG 0.608893 8.4:-61991612 MS.gene87835:CDS
GTCCATATCTCCAATCTGCA+TGG 0.609856 8.4:+61992588 None:intergenic
GGGCCATGGCTTTGCCTACG+GGG 0.610243 8.4:-61991859 MS.gene87835:CDS
CGGGGACAAATCGTGACCAT+AGG 0.613124 8.4:-61991841 MS.gene87835:CDS
AAGCTAGGTTTGATCCTGTG+GGG 0.615240 8.4:-61991673 MS.gene87835:CDS
CCATAGGTATTGCAATCCAA+TGG 0.617062 8.4:-61991825 MS.gene87835:CDS
ACATAGGTTGATTCATAACA+AGG 0.617116 8.4:+61992542 None:intergenic
GATCAAACCTAGCTTCAACC+TGG 0.619274 8.4:+61991681 None:intergenic
ACCAGTGTTGGACATGATGT+GGG 0.635087 8.4:-61991879 MS.gene87835:CDS
ACAAGAATTTGTGACCATGT+TGG 0.638031 8.4:-61991717 MS.gene87835:CDS
GATTGAAACTCAATCCAAAG+TGG 0.656440 8.4:+61993088 None:intergenic
TGTCCCCGTAGGCAAAGCCA+TGG 0.658805 8.4:+61991856 None:intergenic
GACCATGTTGGTCAAATGTG+GGG 0.660571 8.4:-61991705 MS.gene87835:CDS
TGACCCTACTAGAGCTAGTG+CGG 0.675155 8.4:-61991630 MS.gene87835:CDS
TGGTCACGATTTGTCCCCGT+AGG 0.679424 8.4:+61991845 None:intergenic
AAAATGTCAAGAAATTAGGG+AGG 0.680447 8.4:-61991784 MS.gene87835:CDS
CGAATCCTGATGGAGAGCAA+TGG 0.687815 8.4:-61992687 MS.gene87835:CDS
GACCCCACATTTGACCAACA+TGG 0.691227 8.4:+61991703 None:intergenic
AACCCCGAACCTTCACCGGG+GGG 0.694765 8.4:-61993018 MS.gene87835:CDS
GAGACTATGGTGACCCATCG+AGG 0.705564 8.4:-61992660 MS.gene87835:CDS
ACCATCAAACGACAATACCG+TGG 0.707154 8.4:-61992920 MS.gene87835:CDS
AGGGTGTCTTGTAGGCACGG+TGG 0.724739 8.4:+61993052 None:intergenic
CGAACCCCGAACCTTCACCG+GGG 0.756583 8.4:-61993020 MS.gene87835:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAAAATTTCAAACATCTATA+AGG + chr8.4:61992625-61992644 None:intergenic 15.0%
!! TTATGAAAGTAAATCAATAT+CGG + chr8.4:61991820-61991839 None:intergenic 15.0%
!!! ATATTGATTTACTTTCATAA+TGG - chr8.4:61991820-61991839 MS.gene87835:CDS 15.0%
!!! ATTTCATTTTAGTTTTATAG+AGG - chr8.4:61992563-61992582 MS.gene87835:CDS 15.0%
!!! AAAATATATCTTGACTTGTT+TGG - chr8.4:61992314-61992333 MS.gene87835:intron 20.0%
!!! AAGATTCGAACATTTTTTAT+GGG + chr8.4:61991881-61991900 None:intergenic 20.0%
!!! TGATGATGAGAAAAAAATAT+GGG - chr8.4:61991738-61991757 MS.gene87835:CDS 20.0%
!!! TTGATGATGAGAAAAAAATA+TGG - chr8.4:61991737-61991756 MS.gene87835:CDS 20.0%
! AATTTCAAACATCTATAAGG+TGG + chr8.4:61992622-61992641 None:intergenic 25.0%
! ATCATCAATTGTTATGTCTT+TGG + chr8.4:61991725-61991744 None:intergenic 25.0%
! CAAAATTACTCATTATCATG+TGG - chr8.4:61992706-61992725 MS.gene87835:CDS 25.0%
! TGGAAAATGTCAAGAAATTA+GGG - chr8.4:61992919-61992938 MS.gene87835:CDS 25.0%
! TTGATTTACTTTCATAATGG+TGG - chr8.4:61991823-61991842 MS.gene87835:CDS 25.0%
! TTGGAAAATGTCAAGAAATT+AGG - chr8.4:61992918-61992937 MS.gene87835:CDS 25.0%
!! TTTCTTTCTTTTCTTCCAAA+AGG - chr8.4:61992778-61992797 MS.gene87835:CDS 25.0%
!!! CAAGATTCGAACATTTTTTA+TGG + chr8.4:61991882-61991901 None:intergenic 25.0%
AAAATGTCAAGAAATTAGGG+AGG - chr8.4:61992922-61992941 MS.gene87835:CDS 30.0%
GAGAATAACACATATCTCTT+TGG - chr8.4:61992284-61992303 MS.gene87835:intron 30.0%
GTCTTGATTCAAAGAGATTA+AGG + chr8.4:61992687-61992706 None:intergenic 30.0%
GTGGTTTCTAATACAATAAG+AGG + chr8.4:61992603-61992622 None:intergenic 30.0%
TGCAAACATTGTGTTCAATA+CGG - chr8.4:61992095-61992114 MS.gene87835:intron 30.0%
TGCGGTTATAAATATTGCAA+GGG - chr8.4:61993094-61993113 MS.gene87835:CDS 30.0%
TTACTTTCATAATGGTGGTT+GGG - chr8.4:61991828-61991847 MS.gene87835:CDS 30.0%
TTGATTCAAAGAGATTAAGG+TGG + chr8.4:61992684-61992703 None:intergenic 30.0%
TTTACTTTCATAATGGTGGT+TGG - chr8.4:61991827-61991846 MS.gene87835:CDS 30.0%
TTTGATCCATATAACCACTT+TGG - chr8.4:61991604-61991623 MS.gene87835:CDS 30.0%
! AAATATGGGTTCGAATTTTC+AGG - chr8.4:61991752-61991771 MS.gene87835:CDS 30.0%
! AATTATGAACCTTATTTCCG+AGG + chr8.4:61992211-61992230 None:intergenic 30.0%
! ATGAATCAACCTATGTTTTC+TGG - chr8.4:61992171-61992190 MS.gene87835:intron 30.0%
! GTAAGTTCCTGCTATAATTT+TGG - chr8.4:61992425-61992444 MS.gene87835:intron 30.0%
! TAAATGATGATGCCTATGTT+TGG + chr8.4:61992477-61992496 None:intergenic 30.0%
! TTCTTGACATTTTCCAAATG+TGG + chr8.4:61992915-61992934 None:intergenic 30.0%
! TTTAAAGCCAAACCAAACAT+TGG - chr8.4:61992641-61992660 MS.gene87835:CDS 30.0%
!! ACATAGGTTGATTCATAACA+AGG + chr8.4:61992167-61992186 None:intergenic 30.0%
AATCAAATGACGAATCCTGA+TGG - chr8.4:61992009-61992028 MS.gene87835:intron 35.0%
ACAAGAATTTGTGACCATGT+TGG - chr8.4:61992989-61993008 MS.gene87835:CDS 35.0%
CATAAAGATAACACCTCGAT+GGG + chr8.4:61992062-61992081 None:intergenic 35.0%
CATCAAGCCAAAATTATAGC+AGG + chr8.4:61992435-61992454 None:intergenic 35.0%
CTCAATCCAAAGTGGTTATA+TGG + chr8.4:61991613-61991632 None:intergenic 35.0%
GAGAAATCAAGAGACAAATG+AGG + chr8.4:61992520-61992539 None:intergenic 35.0%
GATTGAAACTCAATCCAAAG+TGG + chr8.4:61991621-61991640 None:intergenic 35.0%
GGTCAACCATATCAATATTG+TGG + chr8.4:61993061-61993080 None:intergenic 35.0%
GTGCGGTTATAAATATTGCA+AGG - chr8.4:61993093-61993112 MS.gene87835:CDS 35.0%
TATCTTTATGGATGTGGTTG+TGG - chr8.4:61992072-61992091 MS.gene87835:intron 35.0%
TATTGCAATCCAATGGCAAA+AGG - chr8.4:61992888-61992907 MS.gene87835:CDS 35.0%
TGTTGATCCAATGTTTGGTT+TGG + chr8.4:61992651-61992670 None:intergenic 35.0%
TTCTTCCAAAAGGTTTGCAA+CGG - chr8.4:61992788-61992807 MS.gene87835:CDS 35.0%
! AGGTGTTATCTTTATGGATG+TGG - chr8.4:61992066-61992085 MS.gene87835:intron 35.0%
! CTTTTCTCTCCAGAAAACAT+AGG + chr8.4:61992183-61992202 None:intergenic 35.0%
!! AGTCTTGTTGATCCAATGTT+TGG + chr8.4:61992656-61992675 None:intergenic 35.0%
!! CTATGTTTTCTGGAGAGAAA+AGG - chr8.4:61992181-61992200 MS.gene87835:intron 35.0%
!!! ATTTTGGCTTGATGTGGAAA+TGG - chr8.4:61992441-61992460 MS.gene87835:intron 35.0%
!!! GCTATAATTTTGGCTTGATG+TGG - chr8.4:61992435-61992454 MS.gene87835:intron 35.0%
!!! TCGTTTGATGGTAGTTTTGT+TGG + chr8.4:61991778-61991797 None:intergenic 35.0%
AATGGCAAAAGGACCACATT+TGG - chr8.4:61992899-61992918 MS.gene87835:CDS 40.0%
ATATGGACTTAGAGCCTCTA+AGG - chr8.4:61992133-61992152 MS.gene87835:intron 40.0%
ATCATGTCCAACACTGGTAA+GGG + chr8.4:61992825-61992844 None:intergenic 40.0%
CCATAAAGATAACACCTCGA+TGG + chr8.4:61992063-61992082 None:intergenic 40.0%
CCATTGGATTGCAATACCTA+TGG + chr8.4:61992884-61992903 None:intergenic 40.0%
GGATTGAGTTTCAATCCTGA+CGG - chr8.4:61991625-61991644 MS.gene87835:CDS 40.0%
GGGGTTCCACAATATTGATA+TGG - chr8.4:61993052-61993071 MS.gene87835:CDS 40.0%
TCGAGCATCTTGATATTGTC+CGG + chr8.4:61991968-61991987 None:intergenic 40.0%
TGACCATGTTGGTCAAATGT+GGG - chr8.4:61993000-61993019 MS.gene87835:CDS 40.0%
! AAATGTGGTCCTTTTGCCAT+TGG + chr8.4:61992900-61992919 None:intergenic 40.0%
! ACAAGTTTTCTCGTGTCTCA+AGG - chr8.4:61992349-61992368 MS.gene87835:intron 40.0%
! CCATAGGTATTGCAATCCAA+TGG - chr8.4:61992881-61992900 MS.gene87835:CDS 40.0%
! CCATCGAGGTGTTATCTTTA+TGG - chr8.4:61992060-61992079 MS.gene87835:intron 40.0%
! TTAGAGCCTCTAAGGATTAG+TGG - chr8.4:61992141-61992160 MS.gene87835:intron 40.0%
!! AGGTGTCTTGTTATTGGGTA+TGG - chr8.4:61992942-61992961 MS.gene87835:CDS 40.0%
!! GTTTTCTGGAGAGAAAAGGA+CGG - chr8.4:61992185-61992204 MS.gene87835:intron 40.0%
!! TAGGGAGGTGTCTTGTTATT+GGG - chr8.4:61992937-61992956 MS.gene87835:CDS 40.0%
!! TGTCTTGTTATTGGGTATGG+TGG - chr8.4:61992945-61992964 MS.gene87835:CDS 40.0%
!! TTAGGGAGGTGTCTTGTTAT+TGG - chr8.4:61992936-61992955 MS.gene87835:CDS 40.0%
!!! ATGCTCGAGATGCTATTTTG+TGG - chr8.4:61991980-61991999 MS.gene87835:intron 40.0%
!!! TGCTCGAGATGCTATTTTGT+GGG - chr8.4:61991981-61992000 MS.gene87835:intron 40.0%
AAGCTAGGTTTGATCCTGTG+GGG - chr8.4:61993033-61993052 MS.gene87835:CDS 45.0%
ACAAGGCCACTAATCCTTAG+AGG + chr8.4:61992150-61992169 None:intergenic 45.0%
ACCATCAAACGACAATACCG+TGG - chr8.4:61991786-61991805 MS.gene87835:CDS 45.0%
CATCATGTCCAACACTGGTA+AGG + chr8.4:61992826-61992845 None:intergenic 45.0%
CGAGCATCTTGATATTGTCC+GGG + chr8.4:61991967-61991986 None:intergenic 45.0%
GAAGCTAGGTTTGATCCTGT+GGG - chr8.4:61993032-61993051 MS.gene87835:CDS 45.0%
GACCATGTTGGTCAAATGTG+GGG - chr8.4:61993001-61993020 MS.gene87835:CDS 45.0%
GAGCAATGGTTGAGAGACTA+TGG - chr8.4:61992033-61992052 MS.gene87835:intron 45.0%
GATCAAACCTAGCTTCAACC+TGG + chr8.4:61993028-61993047 None:intergenic 45.0%
GCGACAGAGACAACAATACT+AGG + chr8.4:61991916-61991935 None:intergenic 45.0%
GTCCATATCTCCAATCTGCA+TGG + chr8.4:61992121-61992140 None:intergenic 45.0%
GTGACCATGTTGGTCAAATG+TGG - chr8.4:61992999-61993018 MS.gene87835:CDS 45.0%
TCAATATTGTGGAACCCCAC+AGG + chr8.4:61993050-61993069 None:intergenic 45.0%
TGAAGCTAGGTTTGATCCTG+TGG - chr8.4:61993031-61993050 MS.gene87835:CDS 45.0%
TTCAATACGGCCATGCAGAT+TGG - chr8.4:61992108-61992127 MS.gene87835:intron 45.0%
! ACCAGTGTTGGACATGATGT+GGG - chr8.4:61992827-61992846 MS.gene87835:CDS 45.0%
! TACCAGTGTTGGACATGATG+TGG - chr8.4:61992826-61992845 MS.gene87835:CDS 45.0%
!! ATAACCGCACTAGCTCTAGT+AGG + chr8.4:61993083-61993102 None:intergenic 45.0%
!! TAACCGCACTAGCTCTAGTA+GGG + chr8.4:61993082-61993101 None:intergenic 45.0%
!! TGACTTGTTTGGATGTGCGA+TGG - chr8.4:61992325-61992344 MS.gene87835:intron 45.0%
AAAGGACGGCCTCGGAAATA+AGG - chr8.4:61992199-61992218 MS.gene87835:intron 50.0%
ACACTGGTAAGGGCAAAGTC+TGG + chr8.4:61992815-61992834 None:intergenic 50.0%
CAATATCGGTAAACGCGCCA+CGG + chr8.4:61991806-61991825 None:intergenic 50.0%
CGAATCCTGATGGAGAGCAA+TGG - chr8.4:61992019-61992038 MS.gene87835:intron 50.0%
CTCAACCATTGCTCTCCATC+AGG + chr8.4:61992027-61992046 None:intergenic 50.0%
GACCCCACATTTGACCAACA+TGG + chr8.4:61993006-61993025 None:intergenic 50.0%
GACTTTGCCCTTACCAGTGT+TGG - chr8.4:61992815-61992834 MS.gene87835:CDS 50.0%
GGCACTGCACAACCAAACAT+AGG - chr8.4:61992462-61992481 MS.gene87835:intron 50.0%
GGCCATGCAGATTGGAGATA+TGG - chr8.4:61992116-61992135 MS.gene87835:intron 50.0%
TGACCCTACTAGAGCTAGTG+CGG - chr8.4:61993076-61993095 MS.gene87835:CDS 50.0%
TGTCTCTGTCGCCTTTCGTT+GGG - chr8.4:61991924-61991943 MS.gene87835:intron 50.0%
TTATGGGTACCAGCATCAGC+CGG + chr8.4:61991865-61991884 None:intergenic 50.0%
TTGTCTCTGTCGCCTTTCGT+TGG - chr8.4:61991923-61991942 MS.gene87835:intron 50.0%
! CTGGTCCGTTGCAAACCTTT+TGG + chr8.4:61992796-61992815 None:intergenic 50.0%
! GCCACGGTATTGTCGTTTGA+TGG + chr8.4:61991790-61991809 None:intergenic 50.0%
! TGGGTATGGTGGAGACATCA+TGG - chr8.4:61992956-61992975 MS.gene87835:CDS 50.0%
!! TTGAGGGTGTCTTGTAGGCA+CGG + chr8.4:61991660-61991679 None:intergenic 50.0%
CGGGGACAAATCGTGACCAT+AGG - chr8.4:61992865-61992884 MS.gene87835:CDS 55.0%
GAGACTATGGTGACCCATCG+AGG - chr8.4:61992046-61992065 MS.gene87835:intron 55.0%
GCCCACATCATGTCCAACAC+TGG + chr8.4:61992831-61992850 None:intergenic 55.0%
GTTGGACATGATGTGGGCCA+TGG - chr8.4:61992833-61992852 MS.gene87835:CDS 55.0%
GTTGGTCAAATGTGGGGTCC+AGG - chr8.4:61993007-61993026 MS.gene87835:CDS 55.0%
TATGGGTACCAGCATCAGCC+GGG + chr8.4:61991864-61991883 None:intergenic 55.0%
TGGAGAGAAAAGGACGGCCT+CGG - chr8.4:61992191-61992210 MS.gene87835:intron 55.0%
TGGTCACGATTTGTCCCCGT+AGG + chr8.4:61992864-61992883 None:intergenic 55.0%
TGGTTGGGTCATCCTAAGCC+CGG - chr8.4:61991843-61991862 MS.gene87835:CDS 55.0%
TGTCCGGGTAACCTAGTCTC+TGG + chr8.4:61991952-61991971 None:intergenic 55.0%
!! GTCGGTTGAGGGTGTCTTGT+AGG + chr8.4:61991665-61991684 None:intergenic 55.0%
ACCTTCACCGGGGGGTTCTA+CGG - chr8.4:61991696-61991715 MS.gene87835:CDS 60.0%
AGAACCCCCCGGTGAAGGTT+CGG + chr8.4:61991695-61991714 None:intergenic 60.0%
AGTCTCTGGCGCCCAACGAA+AGG + chr8.4:61991938-61991957 None:intergenic 60.0%
ATCCTAAGCCCGGCTGATGC+TGG - chr8.4:61991853-61991872 MS.gene87835:CDS 60.0%
GCGCCAGAGACTAGGTTACC+CGG - chr8.4:61991946-61991965 MS.gene87835:intron 60.0%
TACCAGCATCAGCCGGGCTT+AGG + chr8.4:61991858-61991877 None:intergenic 60.0%
TGAAGGTTCGGGGTTCGCGT+CGG + chr8.4:61991683-61991702 None:intergenic 60.0%
TGTCCCCGTAGGCAAAGCCA+TGG + chr8.4:61992853-61992872 None:intergenic 60.0%
TTCGTTGGGCGCCAGAGACT+AGG - chr8.4:61991938-61991957 MS.gene87835:intron 60.0%
TTGGACACCGTAGAACCCCC+CGG + chr8.4:61991706-61991725 None:intergenic 60.0%
! AGGGTGTCTTGTAGGCACGG+TGG + chr8.4:61991657-61991676 None:intergenic 60.0%
! GTGGGCCATGGCTTTGCCTA+CGG - chr8.4:61992845-61992864 MS.gene87835:CDS 60.0%
! GTGGGGTCCAGGTTGAAGCT+AGG - chr8.4:61993018-61993037 MS.gene87835:CDS 60.0%
! TGGGCCATGGCTTTGCCTAC+GGG - chr8.4:61992846-61992865 MS.gene87835:CDS 60.0%
AACCCCCCGGTGAAGGTTCG+GGG + chr8.4:61991693-61991712 None:intergenic 65.0%
AACCCCGAACCTTCACCGGG+GGG - chr8.4:61991688-61991707 MS.gene87835:CDS 65.0%
ACCGTAGAACCCCCCGGTGA+AGG + chr8.4:61991700-61991719 None:intergenic 65.0%
CGAACCCCGAACCTTCACCG+GGG - chr8.4:61991686-61991705 MS.gene87835:CDS 65.0%
CGCGAACCCCGAACCTTCAC+CGG - chr8.4:61991684-61991703 MS.gene87835:CDS 65.0%
GAACCCCCCGGTGAAGGTTC+GGG + chr8.4:61991694-61991713 None:intergenic 65.0%
GAACCCCGAACCTTCACCGG+GGG - chr8.4:61991687-61991706 MS.gene87835:CDS 65.0%
GCACGGTGGAGAGTTCCGTC+AGG + chr8.4:61991643-61991662 None:intergenic 65.0%
GCGAACCCCGAACCTTCACC+GGG - chr8.4:61991685-61991704 MS.gene87835:CDS 65.0%
! GGGCCATGGCTTTGCCTACG+GGG - chr8.4:61992847-61992866 MS.gene87835:CDS 65.0%
!! TCGGGGTTCGCGTCGGTTGA+GGG + chr8.4:61991676-61991695 None:intergenic 65.0%
!! TTCGGGGTTCGCGTCGGTTG+AGG + chr8.4:61991677-61991696 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr8.4 gene 61991595 61993133 61991595 ID=MS.gene87835
chr8.4 mRNA 61991595 61993133 61991595 ID=MS.gene87835.t1;Parent=MS.gene87835
chr8.4 exon 61992508 61993133 61992508 ID=MS.gene87835.t1.exon1;Parent=MS.gene87835.t1
chr8.4 CDS 61992508 61993133 61992508 ID=cds.MS.gene87835.t1;Parent=MS.gene87835.t1
chr8.4 exon 61991595 61991928 61991595 ID=MS.gene87835.t1.exon2;Parent=MS.gene87835.t1
chr8.4 CDS 61991595 61991928 61991595 ID=cds.MS.gene87835.t1;Parent=MS.gene87835.t1
Gene Sequence

>MS.gene87835

ATGTCAAAATTTGATCCATATAACCACTTTGGATTGAGTTTCAATCCTGACGGAACTCTCCACCGTGCCTACAAGACACCCTCAACCGACGCGAACCCCGAACCTTCACCGGGGGGTTCTACGGTGTCCAAAGACATAACAATTGATGATGAGAAAAAAATATGGGTTCGAATTTTCAGGCCAACAAAACTACCATCAAACGACAATACCGTGGCGCGTTTACCGATATTGATTTACTTTCATAATGGTGGTTGGGTCATCCTAAGCCCGGCTGATGCTGGTACCCATAAAAAATGTTCGAATCTTGCTTCTGACATTCCTAGTATTGTTGTCTCTGTCGCCTTTCGTTGGGCGCCAGAGACTAGGTTACCCGGACAATATCAAGATGCTCGAGATGCTATTTTGTGGGTGAAAAATCAAATGACGAATCCTGATGGAGAGCAATGGTTGAGAGACTATGGTGACCCATCGAGGTGTTATCTTTATGGATGTGGTTGTGGTGCAAACATTGTGTTCAATACGGCCATGCAGATTGGAGATATGGACTTAGAGCCTCTAAGGATTAGTGGCCTTGTTATGAATCAACCTATGTTTTCTGGAGAGAAAAGGACGGCCTCGGAAATAAGGTTCATAATTTTATCATTCTCTTAAAAAAAAATTGATCAATTATCATTCTCTTAAATAAATTTGAGAATAACACATATCTCTTTGGTTATGAAAAAATATATCTTGACTTGTTTGGATGTGCGATGGAACAAGTTTTCTCGTGTCTCAAGGCAAAAATTGTCATGATTTATAAAAGCTATTATATGTAGCTTCACTGCTAACACGTAAGTTCCTGCTATAATTTTGGCTTGATGTGGAAATGGCACTGCACAACCAAACATAGGCATCATCATTTATTTTTTAAAAAATATTTGCACCTCATTTGTCTCTTGATTTCTCTTGACTATATTCAGTTATGAAGAATTTCATTTTAGTTTTATAGAGGTTTGAAAATTTCTTCCTCTTATTGTATTAGAAACCACCTTATAGATGTTTGAAATTTTAAAGCCAAACCAAACATTGGATCAACAAGACTCTCAACCACCTTAATCTCTTTGAATCAAGACAAAATTACTCATTATCATGTGGTCACGTTATTATTAAATTTTATTTTGCATGAGTCACATTACTTTGTTTTTTTCTTTCTTTTCTTCCAAAAGGTTTGCAACGGACCAGACTTTGCCCTTACCAGTGTTGGACATGATGTGGGCCATGGCTTTGCCTACGGGGACAAATCGTGACCATAGGTATTGCAATCCAATGGCAAAAGGACCACATTTGGAAAATGTCAAGAAATTAGGGAGGTGTCTTGTTATTGGGTATGGTGGAGACATCATGGTTGATCGACAACAAGAATTTGTGACCATGTTGGTCAAATGTGGGGTCCAGGTTGAAGCTAGGTTTGATCCTGTGGGGTTCCACAATATTGATATGGTTGACCCTACTAGAGCTAGTGCGGTTATAAATATTGCAAGGGAGTTTATTATACTTTGA
Protein sequence

>MS.gene87835.t1

MSKFDPYNHFGLSFNPDGTLHRAYKTPSTDANPEPSPGGSTVSKDITIDDEKKIWVRIFRPTKLPSNDNTVARLPILIYFHNGGWVILSPADAGTHKKCSNLASDIPSIVVSVAFRWAPETRLPGQYQDARDAILWVKNQMTNPDGEQWLRDYGDPSRCYLYGCGCGANIVFNTAMQIGDMDLEPLRISGLVMNQPMFSGEKRTASEIRFATDQTLPLPVLDMMWAMALPTGTNRDHRYCNPMAKGPHLENVKKLGRCLVIGYGGDIMVDRQQEFVTMLVKCGVQVEARFDPVGFHNIDMVDPTRASAVINIAREFIIL