Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene20718.t1 | XP_024639656.1 | 96.9 | 127 | 4 | 0 | 1 | 127 | 1 | 127 | 2.60E-37 | 164.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene20718.t1 | A0A396JXZ4 | 96.9 | 127 | 4 | 0 | 1 | 127 | 1 | 127 | 1.9e-37 | 164.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050654 | MS.gene20718 | 0.802173 | 6.36E-49 | -1.69E-46 |
| MS.gene051209 | MS.gene20718 | 0.815181 | 1.05E-51 | -1.69E-46 |
| MS.gene051800 | MS.gene20718 | 0.802957 | 4.38E-49 | -1.69E-46 |
| MS.gene052609 | MS.gene20718 | 0.819641 | 1.04E-52 | -1.69E-46 |
| MS.gene052776 | MS.gene20718 | 0.802575 | 5.26E-49 | -1.69E-46 |
| MS.gene053486 | MS.gene20718 | 0.803019 | 4.26E-49 | -1.69E-46 |
| MS.gene053620 | MS.gene20718 | 0.819649 | 1.04E-52 | -1.69E-46 |
| MS.gene056507 | MS.gene20718 | 0.80871 | 2.71E-50 | -1.69E-46 |
| MS.gene058081 | MS.gene20718 | 0.802075 | 6.66E-49 | -1.69E-46 |
| MS.gene058289 | MS.gene20718 | 0.817735 | 2.83E-52 | -1.69E-46 |
| MS.gene058429 | MS.gene20718 | 0.806493 | 8.01E-50 | -1.69E-46 |
| MS.gene058647 | MS.gene20718 | 0.812651 | 3.81E-51 | -1.69E-46 |
| MS.gene058919 | MS.gene20718 | 0.806863 | 6.69E-50 | -1.69E-46 |
| MS.gene059439 | MS.gene20718 | 0.832477 | 9.24E-56 | -1.69E-46 |
| MS.gene060380 | MS.gene20718 | 0.813295 | 2.75E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene20718.t1 | MTR_1g088730 | 94.488 | 127 | 5 | 1 | 1 | 127 | 1 | 125 | 7.01e-59 | 196 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 27 sgRNAs with CRISPR-Local
Find 43 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATCTAATTCTAAGTCAGCTT+GGG | 0.347117 | 1.2:-66414654 | MS.gene20718:CDS |
| AGGAGAAGAGAGAGGACGTT+TGG | 0.379522 | 1.2:+66415174 | None:intergenic |
| TCACCGTTGGTCAACGAATC+TGG | 0.380371 | 1.2:-66414980 | MS.gene20718:CDS |
| GTGTCTCCTCCTCAGTGTAT+AGG | 0.404471 | 1.2:-66414863 | MS.gene20718:intron |
| TCCAAACGAAGATGAAGCTT+TGG | 0.410208 | 1.2:-66414885 | MS.gene20718:CDS |
| ACCAAAGCTTCATCTTCGTT+TGG | 0.414472 | 1.2:+66414884 | None:intergenic |
| AATCTAATTCTAAGTCAGCT+TGG | 0.423340 | 1.2:-66414655 | MS.gene20718:CDS |
| ACCATCTGATCTGTCAGAAA+TGG | 0.429158 | 1.2:-66414942 | MS.gene20718:CDS |
| GAGTTTGAAGGAGATGATGA+AGG | 0.458380 | 1.2:+66415154 | None:intergenic |
| AGGTGTTACCTCCGTTAGCT+CGG | 0.508029 | 1.2:-66414697 | MS.gene20718:CDS |
| CTCAAAGAATGTTTCATCCA+AGG | 0.514064 | 1.2:-66415011 | MS.gene20718:CDS |
| CTGTCAGAAATGGCTGTTGA+TGG | 0.527659 | 1.2:-66414932 | MS.gene20718:CDS |
| GAGAGGACGTTTGGAAGAAG+AGG | 0.528081 | 1.2:+66415183 | None:intergenic |
| CGTTTCTTCGACCGAGCTAA+CGG | 0.543662 | 1.2:+66414686 | None:intergenic |
| TCTAATTCTAAGTCAGCTTG+GGG | 0.557160 | 1.2:-66414653 | MS.gene20718:CDS |
| CAAGGTTAATCCTTCACCGT+TGG | 0.558411 | 1.2:-66414993 | MS.gene20718:CDS |
| GCTTATTTCTCAATCTTCTG+AGG | 0.564893 | 1.2:-66414627 | MS.gene20718:CDS |
| GCCATTTCTGACAGATCAGA+TGG | 0.570778 | 1.2:+66414941 | None:intergenic |
| CAACGGTGAAGGATTAACCT+TGG | 0.591714 | 1.2:+66414994 | None:intergenic |
| GATTCGTTGACCAACGGTGA+AGG | 0.594433 | 1.2:+66414983 | None:intergenic |
| TCAAATTGCTGAGAAATCAA+AGG | 0.595701 | 1.2:-66414717 | MS.gene20718:CDS |
| GATGATGAAGGAGAAGAGAG+AGG | 0.595870 | 1.2:+66415166 | None:intergenic |
| TCACCAGATTCGTTGACCAA+CGG | 0.601470 | 1.2:+66414977 | None:intergenic |
| TTCTTCGACCGAGCTAACGG+AGG | 0.627508 | 1.2:+66414689 | None:intergenic |
| TTGATACCTATACACTGAGG+AGG | 0.637134 | 1.2:+66414857 | None:intergenic |
| CTAATTCTAAGTCAGCTTGG+GGG | 0.703976 | 1.2:-66414652 | MS.gene20718:CDS |
| ACTTTGATACCTATACACTG+AGG | 0.795812 | 1.2:+66414854 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ACTTTTTATCATTTCTTTCA+TGG | - | chr1.2:66414995-66415014 | MS.gene20718:CDS | 20.0% |
| !!! | CTTTTTATCATTTCTTTCAT+GGG | - | chr1.2:66414996-66415015 | MS.gene20718:CDS | 20.0% |
| !! | TTTTTGCTTTGTGAGATTTA+TGG | - | chr1.2:66415062-66415081 | MS.gene20718:intron | 25.0% |
| !!! | ATAAGGGAAATTATTGGTTT+TGG | + | chr1.2:66414722-66414741 | None:intergenic | 25.0% |
| !!! | ATTTTTGTTTGTGTTGAAAC+AGG | - | chr1.2:66414786-66414805 | MS.gene20718:intron | 25.0% |
| AAACTCAAAAAAACGATCCA+AGG | - | chr1.2:66414684-66414703 | MS.gene20718:CDS | 30.0% | |
| AATCTAATTCTAAGTCAGCT+TGG | - | chr1.2:66415166-66415185 | MS.gene20718:CDS | 30.0% | |
| AATTTACAAACCCATTTGTG+AGG | + | chr1.2:66414746-66414765 | None:intergenic | 30.0% | |
| ATCTAATTCTAAGTCAGCTT+GGG | - | chr1.2:66415167-66415186 | MS.gene20718:CDS | 30.0% | |
| TCAAATTGCTGAGAAATCAA+AGG | - | chr1.2:66415104-66415123 | MS.gene20718:intron | 30.0% | |
| TGAGGAATAAGGGAAATTAT+TGG | + | chr1.2:66414728-66414747 | None:intergenic | 30.0% | |
| ! | ATGGATTTTGTGTTAGAATC+AGG | - | chr1.2:66415081-66415100 | MS.gene20718:intron | 30.0% |
| ! | TTTGGTTTACATAAGAACCT+TGG | + | chr1.2:66414704-66414723 | None:intergenic | 30.0% |
| !!! | GATCGTTTTTTTGAGTTTGA+AGG | + | chr1.2:66414682-66414701 | None:intergenic | 30.0% |
| AAACCCATTTGTGAGGAATA+AGG | + | chr1.2:66414739-66414758 | None:intergenic | 35.0% | |
| AACCCATTTGTGAGGAATAA+GGG | + | chr1.2:66414738-66414757 | None:intergenic | 35.0% | |
| ACTTTGATACCTATACACTG+AGG | + | chr1.2:66414970-66414989 | None:intergenic | 35.0% | |
| ATAATCTAAGCAAGCATGTG+TGG | - | chr1.2:66415037-66415056 | MS.gene20718:intron | 35.0% | |
| CTCAAAGAATGTTTCATCCA+AGG | - | chr1.2:66414810-66414829 | MS.gene20718:intron | 35.0% | |
| TCTAATTCTAAGTCAGCTTG+GGG | - | chr1.2:66415168-66415187 | MS.gene20718:CDS | 35.0% | |
| TTCCCTTATTCCTCACAAAT+GGG | - | chr1.2:66414733-66414752 | MS.gene20718:intron | 35.0% | |
| TTTCCCTTATTCCTCACAAA+TGG | - | chr1.2:66414732-66414751 | MS.gene20718:intron | 35.0% | |
| ! | GCTTATTTCTCAATCTTCTG+AGG | - | chr1.2:66415194-66415213 | MS.gene20718:CDS | 35.0% |
| ACCAAAGCTTCATCTTCGTT+TGG | + | chr1.2:66414940-66414959 | None:intergenic | 40.0% | |
| ACCATCTGATCTGTCAGAAA+TGG | - | chr1.2:66414879-66414898 | MS.gene20718:CDS | 40.0% | |
| CTAATTCTAAGTCAGCTTGG+GGG | - | chr1.2:66415169-66415188 | MS.gene20718:CDS | 40.0% | |
| GAGTTTGAAGGAGATGATGA+AGG | + | chr1.2:66414670-66414689 | None:intergenic | 40.0% | |
| TCCAAACGAAGATGAAGCTT+TGG | - | chr1.2:66414936-66414955 | MS.gene20718:CDS | 40.0% | |
| TTGATACCTATACACTGAGG+AGG | + | chr1.2:66414967-66414986 | None:intergenic | 40.0% | |
| CAACGGTGAAGGATTAACCT+TGG | + | chr1.2:66414830-66414849 | None:intergenic | 45.0% | |
| CAAGGTTAATCCTTCACCGT+TGG | - | chr1.2:66414828-66414847 | MS.gene20718:intron | 45.0% | |
| CTGTCAGAAATGGCTGTTGA+TGG | - | chr1.2:66414889-66414908 | MS.gene20718:CDS | 45.0% | |
| GATGATGAAGGAGAAGAGAG+AGG | + | chr1.2:66414658-66414677 | None:intergenic | 45.0% | |
| GCCATTTCTGACAGATCAGA+TGG | + | chr1.2:66414883-66414902 | None:intergenic | 45.0% | |
| TCACCAGATTCGTTGACCAA+CGG | + | chr1.2:66414847-66414866 | None:intergenic | 45.0% | |
| AGGAGAAGAGAGAGGACGTT+TGG | + | chr1.2:66414650-66414669 | None:intergenic | 50.0% | |
| CGTTTCTTCGACCGAGCTAA+CGG | + | chr1.2:66415138-66415157 | None:intergenic | 50.0% | |
| GAGAGGACGTTTGGAAGAAG+AGG | + | chr1.2:66414641-66414660 | None:intergenic | 50.0% | |
| GATTCGTTGACCAACGGTGA+AGG | + | chr1.2:66414841-66414860 | None:intergenic | 50.0% | |
| GTGTCTCCTCCTCAGTGTAT+AGG | - | chr1.2:66414958-66414977 | MS.gene20718:CDS | 50.0% | |
| TCACCGTTGGTCAACGAATC+TGG | - | chr1.2:66414841-66414860 | MS.gene20718:intron | 50.0% | |
| ! | AGGTGTTACCTCCGTTAGCT+CGG | - | chr1.2:66415124-66415143 | MS.gene20718:intron | 50.0% |
| TTCTTCGACCGAGCTAACGG+AGG | + | chr1.2:66415135-66415154 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.2 | gene | 66414616 | 66415227 | 66414616 | ID=MS.gene20718 |
| chr1.2 | mRNA | 66414616 | 66415227 | 66414616 | ID=MS.gene20718.t1;Parent=MS.gene20718 |
| chr1.2 | exon | 66415138 | 66415227 | 66415138 | ID=MS.gene20718.t1.exon1;Parent=MS.gene20718.t1 |
| chr1.2 | CDS | 66415138 | 66415227 | 66415138 | ID=cds.MS.gene20718.t1;Parent=MS.gene20718.t1 |
| chr1.2 | exon | 66414864 | 66415035 | 66414864 | ID=MS.gene20718.t1.exon2;Parent=MS.gene20718.t1 |
| chr1.2 | CDS | 66414864 | 66415035 | 66414864 | ID=cds.MS.gene20718.t1;Parent=MS.gene20718.t1 |
| chr1.2 | exon | 66414616 | 66414746 | 66414616 | ID=MS.gene20718.t1.exon3;Parent=MS.gene20718.t1 |
| chr1.2 | CDS | 66414616 | 66414746 | 66414616 | ID=cds.MS.gene20718.t1;Parent=MS.gene20718.t1 |
| Gene Sequence |
| Protein sequence |
