Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene21025.t1 | XP_003591401.1 | 93.9 | 82 | 5 | 0 | 1 | 82 | 50 | 131 | 1.90E-33 | 151.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene21025.t1 | Q9LZP9 | 70.9 | 86 | 21 | 2 | 1 | 82 | 46 | 131 | 9.4e-26 | 117.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene21025.t1 | B7FGI5 | 93.9 | 82 | 5 | 0 | 1 | 82 | 50 | 131 | 1.3e-33 | 151.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050404 | MS.gene21025 | 0.814527 | 1.47E-51 | -1.69E-46 |
| MS.gene050406 | MS.gene21025 | 0.82258 | 2.20E-53 | -1.69E-46 |
| MS.gene051171 | MS.gene21025 | 0.802839 | 4.64E-49 | -1.69E-46 |
| MS.gene053330 | MS.gene21025 | 0.830485 | 2.86E-55 | -1.69E-46 |
| MS.gene055370 | MS.gene21025 | 0.812051 | 5.15E-51 | -1.69E-46 |
| MS.gene056633 | MS.gene21025 | 0.817215 | 3.70E-52 | -1.69E-46 |
| MS.gene058746 | MS.gene21025 | 0.819311 | 1.24E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene21025.t1 | MTR_1g087030 | 93.902 | 82 | 5 | 0 | 1 | 82 | 50 | 131 | 5.03e-52 | 159 |
| MS.gene21025.t1 | MTR_7g109520 | 73.171 | 82 | 21 | 1 | 1 | 81 | 46 | 127 | 1.66e-38 | 124 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene21025.t1 | AT3G62410 | 70.930 | 86 | 21 | 2 | 1 | 82 | 46 | 131 | 1.61e-37 | 122 |
| MS.gene21025.t1 | AT2G47400 | 73.973 | 73 | 17 | 1 | 12 | 82 | 52 | 124 | 3.23e-31 | 106 |
| MS.gene21025.t1 | AT1G76560 | 48.611 | 72 | 35 | 1 | 13 | 82 | 63 | 134 | 1.27e-17 | 72.0 |
Find 0 sgRNAs with CRISPR-Local
Find 31 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAGGGATAAGAAAAAGACGT+CGG | + | 11140:10294-10313 | MS.gene21025:CDS | 35.0% | |
| ! | TCTTTTTCTTATCCCTTGCA+TGG | - | 11140:10291-10310 | MS.gene21025:intergenic | 35.0% |
| GGAATACTGCAAGGACAATC+CGG | + | 11140:10327-10346 | MS.gene21025:CDS | 45.0% | |
| ! | GAGCATAAAGAGTGCAGAAG+AGG | + | 11140:10189-10208 | MS.gene21025:CDS | 45.0% |
| AAGCATCACCGGACATAACC+GGG | + | 11140:10155-10174 | MS.gene21025:CDS | 50.0% | |
| ATACTGCAAGGACAATCCGG+AGG | + | 11140:10330-10349 | MS.gene21025:CDS | 50.0% | |
| CAAGCATCACCGGACATAAC+CGG | + | 11140:10154-10173 | MS.gene21025:CDS | 50.0% | |
| CCTTGCAGTATTCCTCGAGA+GGG | - | 11140:10321-10340 | MS.gene21025:intergenic | 50.0% | |
| TATGCTCTCTTCCACCTTCC+CGG | - | 11140:10176-10195 | MS.gene21025:intergenic | 50.0% | |
| TCCTTGCAGTATTCCTCGAG+AGG | - | 11140:10322-10341 | MS.gene21025:intergenic | 50.0% | |
| TGAGGTTGAGGAACTGAGTG+CGG | + | 11140:10258-10277 | MS.gene21025:CDS | 50.0% | |
| ! | GTACGACACTCATTAGCCTC+CGG | - | 11140:10349-10368 | MS.gene21025:intergenic | 50.0% |
| ATCACCGGACATAACCGGGA+AGG | + | 11140:10159-10178 | MS.gene21025:CDS | 55.0% | |
| ATGTCCGGTGATGCTTGCAC+CGG | - | 11140:10151-10170 | MS.gene21025:intergenic | 55.0% | |
| CCCTCTCGAGGAATACTGCA+AGG | + | 11140:10318-10337 | MS.gene21025:CDS | 55.0% | |
| TCCACCTTCCCGGTTATGTC+CGG | - | 11140:10166-10185 | MS.gene21025:intergenic | 55.0% | |
| AAAGACGTCGGACCCTCTCG+AGG | + | 11140:10306-10325 | MS.gene21025:CDS | 60.0% | |
| ACCGGACATAACCGGGAAGG+TGG | + | 11140:10162-10181 | MS.gene21025:CDS | 60.0% | |
| CGCCACACATTCACCGCTAG+CGG | - | 11140:10230-10249 | MS.gene21025:intergenic | 60.0% | |
| CGCTAGCGGTGAATGTGTGG+CGG | + | 11140:10228-10247 | MS.gene21025:CDS | 60.0% | |
| GGTTGAGGAACTGAGTGCGG+CGG | + | 11140:10261-10280 | MS.gene21025:CDS | 60.0% | |
| TCGACCGGTGCAAGCATCAC+CGG | + | 11140:10144-10163 | MS.gene21025:CDS | 60.0% | |
| ! | AGTGCAGAAGAGGCGTGTGC+AGG | + | 11140:10199-10218 | MS.gene21025:CDS | 60.0% |
| ATGTGTGGCGGCGTGGGATG+AGG | + | 11140:10240-10259 | MS.gene21025:CDS | 65.0% | |
| CGCCGCTAGCGGTGAATGTG+TGG | + | 11140:10225-10244 | MS.gene21025:CDS | 65.0% | |
| CGGTGAATGTGTGGCGGCGT+GGG | + | 11140:10234-10253 | MS.gene21025:CDS | 65.0% | |
| TGCGGCGGCAAGCCATGCAA+GGG | + | 11140:10276-10295 | MS.gene21025:CDS | 65.0% | |
| TGTGCAGGAGACGCCGCTAG+CGG | + | 11140:10214-10233 | MS.gene21025:CDS | 65.0% | |
| GCGGTGAATGTGTGGCGGCG+TGG | + | 11140:10233-10252 | MS.gene21025:CDS | 70.0% | |
| GGCGGCGTGGGATGAGGTTG+AGG | + | 11140:10246-10265 | MS.gene21025:CDS | 70.0% | |
| GTGCGGCGGCAAGCCATGCA+AGG | + | 11140:10275-10294 | MS.gene21025:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 11140 | gene | 10133 | 10381 | 10133 | ID=MS.gene21025 |
| 11140 | mRNA | 10133 | 10381 | 10133 | ID=MS.gene21025.t1;Parent=MS.gene21025 |
| 11140 | exon | 10133 | 10381 | 10133 | ID=MS.gene21025.t1.exon1;Parent=MS.gene21025.t1 |
| 11140 | CDS | 10133 | 10381 | 10133 | ID=cds.MS.gene21025.t1;Parent=MS.gene21025.t1 |
| Gene Sequence |
| Protein sequence |
