AlfalfaGEDB | MS.gene21175

M. sativa cultivar XinJiangDaYe / MS.gene21175

KEGG: K02983
GO: GO:0003674 GO:0003735 GO:0005198 GO:0005575 GO:0005840 GO:0006412 GO:0006518 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0019538 GO:0034641 GO:0034645 GO:0043043 GO:0043170 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0071704 GO:0110165 GO:1901564 GO:1901566 GO:1901576
PFAM: PF04758.17

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene21175.t1	XP_003608708.1	100	62	0	0	1	62	1	62	4.10E-25	123.2

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene21175.t1	P49689	95.2	62	3	0	1	62	1	62	2.4e-26	118.6

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene21175.t1	G7JW33	100.0	62	0	0	1	62	1	62	3.0e-25	123.2

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene057237	MS.gene21175	0.823349	1.45E-53	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene21175	MS.gene68716	PPI
MS.gene21175	MS.gene063067	PPI
MS.gene68113	MS.gene21175	PPI
MS.gene21175	MS.gene014181	PPI
MS.gene21175	MS.gene71949	PPI
MS.gene71949	MS.gene21175	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene21175.t1	MTR_5g012610	100.000	62	0	0	1	62	1	62	2.81e-39	123
MS.gene21175.t1	MTR_4g100900	100.000	62	0	0	1	62	1	62	2.81e-39	123

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene21175.t1	AT5G56670	95.161	62	3	1	62	1	62	6.25e-30	100
MS.gene21175.t1	AT4G29390	95.161	62	3	1	62	1	62	6.25e-30	100
MS.gene21175.t1	AT2G19750	95.161	62	3	1	62	1	62	6.25e-30	100

Find 20 sgRNAs with CRISPR-Local

Find 27 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GCTTTGCTACCTTTGGTGTT+TGG	0.335294	5.1:+5781526	None:intergenic
TTGTCTTGCTTTGCTACCTT+TGG	0.348505	5.1:+5781519	None:intergenic
TGTTTGGCCTCTAACCTTAC+CGG	0.358772	5.1:+5781542	None:intergenic
TCTTACTTCTCAGATGAGTT+TGG	0.367396	5.1:+5781305	None:intergenic
GTTACTGTAGTTGTTGGATT+CGG	0.419179	5.1:-5781341	MS.gene21175:intron
GCCTCTAACCTTACCGGCAC+GGG	0.419662	5.1:+5781548	None:intergenic
ATCGTTGGCCCGTGCCGGTA+AGG	0.433030	5.1:-5781556	MS.gene21175:CDS
GCCCGTGCCGGTAAGGTTAG+AGG	0.445776	5.1:-5781549	MS.gene21175:CDS
ACGCTTGTGAGCACGGCCAC+GGG	0.451262	5.1:+5781485	None:intergenic
CACGGATCGTTGGCCCGTGC+CGG	0.457604	5.1:-5781561	MS.gene21175:CDS
TACGCTTGTGAGCACGGCCA+CGG	0.461093	5.1:+5781484	None:intergenic
GGCCTCTAACCTTACCGGCA+CGG	0.499402	5.1:+5781547	None:intergenic
ACTGCATACGCTTGTGAGCA+CGG	0.575185	5.1:+5781478	None:intergenic
CGCTTGTGAGCACGGCCACG+GGG	0.579942	5.1:+5781486	None:intergenic
AGGAAAGGTTCACGGATCGT+TGG	0.583409	5.1:-5781571	MS.gene21175:CDS
TACCGGCAGTGACGAATCTG+CGG	0.585546	5.1:+5781451	None:intergenic
AACCGCAGATTCGTCACTGC+CGG	0.593013	5.1:-5781453	MS.gene21175:intron
GTTGGATTCGGTAAGAAGAG+AGG	0.609691	5.1:-5781329	MS.gene21175:CDS
GGTTAGAGGCCAAACACCAA+AGG	0.645954	5.1:-5781535	MS.gene21175:CDS
GACAAGAAGAAGCAGCCCCG+TGG	0.665991	5.1:-5781501	MS.gene21175:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	ATGAATAAATCTAAAAAATT+AGG	+	chr5.1:5781564-5781583	None:intergenic	10.0%
!!	TGAATAAATCTAAAAAATTA+GGG	+	chr5.1:5781563-5781582	None:intergenic	10.0%
	ATTCTTGTTACTGTAGTTGT+TGG	-	chr5.1:5781630-5781649	MS.gene21175:intron	30.0%
	TCTAAAAAATTAGGGTTTCG+AGG	+	chr5.1:5781555-5781574	None:intergenic	30.0%
!!	AACAATGAAATCGATTTTGC+AGG	-	chr5.1:5781386-5781405	MS.gene21175:intron	30.0%
	GTTACTGTAGTTGTTGGATT+CGG	-	chr5.1:5781636-5781655	MS.gene21175:intron	35.0%
!!	TGAAATCGATTTTGCAGGAA+AGG	-	chr5.1:5781391-5781410	MS.gene21175:intron	35.0%
	TTGTCTTGCTTTGCTACCTT+TGG	+	chr5.1:5781461-5781480	None:intergenic	40.0%
!	GATTTTGCAGGAAAGGTTCA+CGG	-	chr5.1:5781398-5781417	MS.gene21175:intron	40.0%
!	TTAGGGTTTCGAGGTATTAC+CGG	+	chr5.1:5781546-5781565	None:intergenic	40.0%
	TGTTTGGCCTCTAACCTTAC+CGG	+	chr5.1:5781438-5781457	None:intergenic	45.0%
!	GTTGGATTCGGTAAGAAGAG+AGG	-	chr5.1:5781648-5781667	MS.gene21175:intron	45.0%
!!	GCTTTGCTACCTTTGGTGTT+TGG	+	chr5.1:5781454-5781473	None:intergenic	45.0%
	ACTGCATACGCTTGTGAGCA+CGG	+	chr5.1:5781502-5781521	None:intergenic	50.0%
	AGGAAAGGTTCACGGATCGT+TGG	-	chr5.1:5781406-5781425	MS.gene21175:intron	50.0%
	GGTTAGAGGCCAAACACCAA+AGG	-	chr5.1:5781442-5781461	MS.gene21175:intron	50.0%
	AACCGCAGATTCGTCACTGC+CGG	-	chr5.1:5781524-5781543	MS.gene21175:CDS	55.0%
	TACCGGCAGTGACGAATCTG+CGG	+	chr5.1:5781529-5781548	None:intergenic	55.0%
	GACAAGAAGAAGCAGCCCCG+TGG	-	chr5.1:5781476-5781495	MS.gene21175:CDS	60.0%
	GCCTCTAACCTTACCGGCAC+GGG	+	chr5.1:5781432-5781451	None:intergenic	60.0%
	GGCCTCTAACCTTACCGGCA+CGG	+	chr5.1:5781433-5781452	None:intergenic	60.0%
	TACGCTTGTGAGCACGGCCA+CGG	+	chr5.1:5781496-5781515	None:intergenic	60.0%
	ACGCTTGTGAGCACGGCCAC+GGG	+	chr5.1:5781495-5781514	None:intergenic	65.0%
!	ATCGTTGGCCCGTGCCGGTA+AGG	-	chr5.1:5781421-5781440	MS.gene21175:intron	65.0%
!	GCCCGTGCCGGTAAGGTTAG+AGG	-	chr5.1:5781428-5781447	MS.gene21175:intron	65.0%
	CACGGATCGTTGGCCCGTGC+CGG	-	chr5.1:5781416-5781435	MS.gene21175:intron	70.0%
	CGCTTGTGAGCACGGCCACG+GGG	+	chr5.1:5781494-5781513	None:intergenic	70.0%

Chromosome	Type	Strat	End	Strand	Name
chr5.1	gene	5781307	5781692	5781307	ID=MS.gene21175
chr5.1	mRNA	5781307	5781692	5781307	ID=MS.gene21175.t1;Parent=MS.gene21175
chr5.1	exon	5781689	5781692	5781689	ID=MS.gene21175.t1.exon1;Parent=MS.gene21175.t1
chr5.1	CDS	5781689	5781692	5781689	ID=cds.MS.gene21175.t1;Parent=MS.gene21175.t1
chr5.1	exon	5781454	5781591	5781454	ID=MS.gene21175.t1.exon2;Parent=MS.gene21175.t1
chr5.1	CDS	5781454	5781591	5781454	ID=cds.MS.gene21175.t1;Parent=MS.gene21175.t1
chr5.1	exon	5781307	5781353	5781307	ID=MS.gene21175.t1.exon3;Parent=MS.gene21175.t1
chr5.1	CDS	5781307	5781353	5781307	ID=cds.MS.gene21175.t1;Parent=MS.gene21175.t1

Gene Sequence

>MS.gene21175

ATGGGTAAGTTAAATTGCATTTTAATAAACGATGTGCGATGTTGTCGTTTAATTTACTTTTCGTGTATAATTGATTATAAACAATGAAATCGATTTTGCAGGAAAGGTTCACGGATCGTTGGCCCGTGCCGGTAAGGTTAGAGGCCAAACACCAAAGGTAGCAAAGCAAGACAAGAAGAAGCAGCCCCGTGGCCGTGCTCACAAGCGTATGCAGTACAACCGCAGATTCGTCACTGCCGGTAATACCTCGAAACCCTAATTTTTTAGATTTATTCATATTATTAAATTAATTATTATTTTTGCTTTTCTGTTGTGTGATTTTGATTCTTGTTACTGTAGTTGTTGGATTCGGTAAGAAGAGAGGACCAAACTCATCTGAGAAGTAA

Protein sequence

>MS.gene21175.t1

MGKVHGSLARAGKVRGQTPKVAKQDKKKQPRGRAHKRMQYNRRFVTAVVGFGKKRGPNSSEK