AlfalfaGEDB | MS.gene68113

M. sativa cultivar XinJiangDaYe / MS.gene68113

KEGG: K06067
GO: GO:0003674 GO:0003824 GO:0004407 GO:0006325 GO:0006464 GO:0006476 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0016043 GO:0016569 GO:0016570 GO:0016575 GO:0016787 GO:0016810 GO:0016811 GO:0019213 GO:0019538 GO:0033558 GO:0035601 GO:0036211 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0071704 GO:0071840 GO:0098732 GO:0140096 GO:1901564
PFAM: PF00850.22

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene68113.t1	XP_013462369.1	98.6	498	7	0	1	498	1	498	9.80E-279	968.8

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene68113.t1	O22446	83.5	486	78	2	1	485	1	485	1.9e-230	799.7

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene68113.t1	A0A072V336	98.6	498	7	0	1	498	1	498	7.1e-279	968.8

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene051122	MS.gene68113	-0.812493	4.12E-51	-1.69E-46
MS.gene055701	MS.gene68113	-0.80589	1.07E-49	-1.69E-46
MS.gene057156	MS.gene68113	0.801091	1.06E-48	-1.69E-46
MS.gene060850	MS.gene68113	-0.813326	2.71E-51	-1.69E-46
MS.gene060851	MS.gene68113	-0.837394	5.33E-57	-1.69E-46
MS.gene060852	MS.gene68113	-0.836389	9.63E-57	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene68113	MS.gene045265	PPI
MS.gene28432	MS.gene68113	PPI
MS.gene68113	MS.gene68079	PPI
MS.gene68113	MS.gene46303	PPI
MS.gene68113	MS.gene28438	PPI
MS.gene68113	MS.gene89672	PPI
MS.gene80820	MS.gene68113	PPI
MS.gene05640	MS.gene68113	PPI
MS.gene68113	MS.gene09204	PPI
MS.gene020313	MS.gene68113	PPI
MS.gene68113	MS.gene91631	PPI
MS.gene68113	MS.gene29637	PPI
MS.gene68113	MS.gene90689	PPI
MS.gene68113	MS.gene000865	PPI
MS.gene68113	MS.gene04748	PPI
MS.gene68113	MS.gene59883	PPI
MS.gene68113	MS.gene066830	PPI
MS.gene68113	MS.gene017622	PPI
MS.gene28434	MS.gene68113	PPI
MS.gene03199	MS.gene68113	PPI
MS.gene27302	MS.gene68113	PPI
MS.gene97701	MS.gene68113	PPI
MS.gene68113	MS.gene016544	PPI
MS.gene68113	MS.gene030661	PPI
MS.gene68113	MS.gene26816	PPI
MS.gene68113	MS.gene26406	PPI
MS.gene68113	MS.gene063809	PPI
MS.gene68113	MS.gene060311	PPI
MS.gene68113	MS.gene70661	PPI
MS.gene68113	MS.gene70990	PPI
MS.gene68113	MS.gene64789	PPI
MS.gene68113	MS.gene068179	PPI
MS.gene68113	MS.gene02570	PPI
MS.gene68113	MS.gene68598	PPI
MS.gene68113	MS.gene027909	PPI
MS.gene68113	MS.gene014890	PPI
MS.gene68113	MS.gene70261	PPI
MS.gene68113	MS.gene009155	PPI
MS.gene68113	MS.gene018762	PPI
MS.gene68113	MS.gene61084	PPI
MS.gene68113	MS.gene97703	PPI
MS.gene68113	MS.gene71660	PPI
MS.gene68113	MS.gene041167	PPI
MS.gene68113	MS.gene051698	PPI
MS.gene68113	MS.gene016292	PPI
MS.gene68113	MS.gene58114	PPI
MS.gene68113	MS.gene030664	PPI
MS.gene68113	MS.gene32685	PPI
MS.gene68113	MS.gene80202	PPI
MS.gene68113	MS.gene047701	PPI
MS.gene68113	MS.gene030666	PPI
MS.gene68113	MS.gene013784	PPI
MS.gene68113	MS.gene21175	PPI
MS.gene68113	MS.gene04418	PPI
MS.gene68113	MS.gene05905	PPI
MS.gene68113	MS.gene66051	PPI
MS.gene68113	MS.gene04820	PPI
MS.gene68113	MS.gene024064	PPI
MS.gene68113	MS.gene55174	PPI
MS.gene68113	MS.gene64164	PPI
MS.gene68113	MS.gene78840	PPI
MS.gene68113	MS.gene86409	PPI
MS.gene68113	MS.gene08109	PPI
MS.gene68113	MS.gene71949	PPI
MS.gene68113	MS.gene67460	PPI
MS.gene68113	MS.gene78924	PPI
MS.gene68113	MS.gene057458	PPI
MS.gene68113	MS.gene012127	PPI
MS.gene68113	MS.gene009411	PPI
MS.gene049111	MS.gene68113	PPI
MS.gene68113	MS.gene39538	PPI
MS.gene70263	MS.gene68113	PPI
MS.gene68113	MS.gene58112	PPI
MS.gene68113	MS.gene071851	PPI
MS.gene68113	MS.gene09038	PPI
MS.gene68113	MS.gene23694	PPI
MS.gene68113	MS.gene04079	PPI
MS.gene68113	MS.gene022334	PPI
MS.gene68113	MS.gene52605	PPI
MS.gene68113	MS.gene00233	PPI
MS.gene68113	MS.gene56214	PPI
MS.gene68113	MS.gene068178	PPI
MS.gene68113	MS.gene029611	PPI
MS.gene68113	MS.gene76850	PPI
MS.gene68113	MS.gene59485	PPI
MS.gene68113	MS.gene47573	PPI
MS.gene68113	MS.gene78117	PPI
MS.gene68113	MS.gene027904	PPI
MS.gene68113	MS.gene25997	PPI
MS.gene68113	MS.gene059054	PPI
MS.gene68113	MS.gene88440	PPI
MS.gene68113	MS.gene83541	PPI
MS.gene68113	MS.gene44230	PPI
MS.gene68113	MS.gene010426	PPI
MS.gene68113	MS.gene058374	PPI
MS.gene68113	MS.gene80691	PPI
MS.gene68113	MS.gene26084	PPI
MS.gene68113	MS.gene26972	PPI
MS.gene68113	MS.gene017431	PPI
MS.gene68113	MS.gene78227	PPI
MS.gene68113	MS.gene70258	PPI
MS.gene68113	MS.gene65125	PPI
MS.gene68113	MS.gene061229	PPI
MS.gene68113	MS.gene75598	PPI
MS.gene68113	MS.gene056335	PPI
MS.gene68113	MS.gene021887	PPI
MS.gene58285	MS.gene68113	PPI
MS.gene68113	MS.gene067266	PPI
MS.gene68113	MS.gene46262	PPI
MS.gene68113	MS.gene066925	PPI
MS.gene68113	MS.gene74499	PPI
MS.gene68113	MS.gene23886	PPI
MS.gene68113	MS.gene015760	PPI
MS.gene066811	MS.gene68113	PPI
MS.gene68113	MS.gene89203	PPI
MS.gene68113	MS.gene24268	PPI
MS.gene027907	MS.gene68113	PPI
MS.gene68113	MS.gene067412	PPI
MS.gene68113	MS.gene29445	PPI
MS.gene68113	MS.gene58116	PPI
MS.gene68113	MS.gene78224	PPI
MS.gene68113	MS.gene038612	PPI
MS.gene08434	MS.gene68113	PPI
MS.gene68113	MS.gene033505	PPI
MS.gene68113	MS.gene78922	PPI
MS.gene68113	MS.gene59884	PPI
MS.gene030125	MS.gene68113	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene68113.t1	MTR_3g118535	98.594	498	7	0	1	498	1	498	0.0	1015
MS.gene68113.t1	MTR_5g004640	84.354	441	67	2	5	445	9	447	0.0	744
MS.gene68113.t1	MTR_3g077160	62.857	455	155	4	5	445	13	467	0.0	608
MS.gene68113.t1	MTR_4g055080	58.495	412	167	2	17	424	4	415	0.0	514
MS.gene68113.t1	MTR_4g055080	58.974	390	156	2	39	424	1	390	3.24e-171	488
MS.gene68113.t1	MTR_3g077120	66.102	177	56	1	5	177	13	189	1.23e-82	254
MS.gene68113.t1	MTR_2g087270	35.165	182	108	3	149	320	154	335	1.02e-25	108
MS.gene68113.t1	MTR_4g109500	26.032	315	208	9	19	319	26	329	8.62e-24	105
MS.gene68113.t1	MTR_4g109500	26.032	315	208	9	19	319	26	329	1.08e-23	105
MS.gene68113.t1	MTR_4g111985	26.115	314	210	12	37	338	257	560	5.32e-22	100
MS.gene68113.t1	MTR_4g111985	26.115	314	210	12	37	338	218	521	8.87e-22	99.0
MS.gene68113.t1	MTR_4g111985	26.855	283	188	11	37	310	218	490	2.18e-19	91.3
MS.gene68113.t1	MTR_1g016440	26.990	289	191	8	37	319	52	326	1.40e-18	87.4
MS.gene68113.t1	MTR_8g093800	27.473	273	165	9	67	318	113	373	1.84e-18	87.8

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene68113.t1	AT4G38130	81.893	486	86	2	1	485	1	485	0.0	789
MS.gene68113.t1	AT4G38130	84.052	464	73	1	1	464	1	463	0.0	781
MS.gene68113.t1	AT5G63110	68.856	411	124	3	2	409	4	413	0.0	597
MS.gene68113.t1	AT3G44680	57.908	411	171	2	17	426	4	413	0.0	514
MS.gene68113.t1	AT5G35600	55.501	409	171	4	10	411	2	406	1.57e-161	464
MS.gene68113.t1	AT3G44490	44.138	145	80	1	282	426	2	145	1.29e-38	138
MS.gene68113.t1	AT1G08460	27.723	303	185	6	37	320	39	326	3.86e-28	115
MS.gene68113.t1	AT3G44660	39.310	145	71	2	282	426	2	129	6.99e-28	108
MS.gene68113.t1	AT5G61060	29.503	322	201	11	17	319	24	338	1.64e-27	117
MS.gene68113.t1	AT5G61060	29.448	326	200	11	17	319	24	342	2.81e-26	113
MS.gene68113.t1	AT5G61070	27.900	319	210	10	17	319	57	371	1.27e-24	108
MS.gene68113.t1	AT1G08460	27.925	265	160	6	24	274	28	275	1.86e-24	104
MS.gene68113.t1	AT3G18520	26.596	376	223	12	34	381	168	518	6.78e-23	102
MS.gene68113.t1	AT3G18520	26.596	376	223	12	34	381	168	518	9.92e-23	101
MS.gene68113.t1	AT3G18520	28.333	240	162	5	149	378	276	515	2.33e-22	100
MS.gene68113.t1	AT3G18520	31.579	171	112	4	149	314	276	446	9.41e-19	89.4
MS.gene68113.t1	AT4G33470	33.594	128	82	2	149	274	201	327	2.72e-17	84.3
MS.gene68113.t1	AT5G26040	25.597	293	192	10	37	320	93	368	1.05e-15	79.3

Find 126 sgRNAs with CRISPR-Local

Find 0 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TCCATTACTTTGCCGATTAA+AGG	0.164939	3.4:-100346449	None:intergenic
GAAAATAATCACCAAATTTA+TGG	0.167816	3.4:-100346316	None:intergenic
GATGCTTTAATCTTTCAATT+AGG	0.168446	3.4:+100346537	MS.gene68113:CDS
ATATGATCCTGTTGTGTTTC+AGG	0.173298	3.4:-100343502	None:intergenic
TGCTCCCAGTGTTCAATTTC+AGG	0.211713	3.4:+100347285	MS.gene68113:CDS
ACTGTTTCTTTCCATAAATT+TGG	0.213488	3.4:+100346305	MS.gene68113:CDS
ATGCTTTAATCTTTCAATTA+GGG	0.231751	3.4:+100346538	MS.gene68113:CDS
TCCAGAAGCCTCACACTTCT+TGG	0.274591	3.4:-100343686	None:intergenic
AATGTGATATTGCTGTTAAC+TGG	0.302265	3.4:+100343644	MS.gene68113:CDS
CATGCCCTTCTTGCCCACTA+TGG	0.309164	3.4:+100343376	MS.gene68113:CDS
GCCAAGAAGTGTGAGGCTTC+TGG	0.309292	3.4:+100343685	MS.gene68113:CDS
TATGATCCTGTTGTGTTTCA+GGG	0.318503	3.4:-100343501	None:intergenic
GAAGATTGTCCTGTCTTTGA+TGG	0.327128	3.4:+100343553	MS.gene68113:CDS
GAGCATTGGGATCCAGATTC+TGG	0.330640	3.4:+100348062	MS.gene68113:CDS
CACGAGTATTATGAATATTT+CGG	0.338501	3.4:+100347151	MS.gene68113:CDS
TTTCTCTCTTGACTTTGCTT+TGG	0.363823	3.4:-100348197	None:intergenic
TCAATGTCCATGCCAGAATC+TGG	0.374372	3.4:-100348074	None:intergenic
AGGCCTCCTGATTCTGATCT+TGG	0.377155	3.4:+100347310	MS.gene68113:CDS
TTCACATTATCTTCATCAAC+TGG	0.383240	3.4:-100352266	None:intergenic
GCAAAGTAATGGAAATCTTT+AGG	0.383254	3.4:+100346459	MS.gene68113:CDS
TGTGGTGCAGACTCTTTATC+TGG	0.383462	3.4:+100346503	MS.gene68113:CDS
ACTGGCGACTTGCGTGATAT+TGG	0.384986	3.4:+100346344	MS.gene68113:CDS
TTGTTGTTCAAACCTTTAAT+CGG	0.387133	3.4:+100346437	MS.gene68113:CDS
GCGGATACGATGTGGCTTCA+TGG	0.387603	3.4:-100343347	None:intergenic
CTTCCGTCTTCTGCACCTGA+TGG	0.389623	3.4:+100343256	MS.gene68113:CDS
GAGGTTGGGAATTATTATTA+TGG	0.397863	3.4:+100343313	MS.gene68113:CDS
AGGCAGACGGGAGTTGCTCT+TGG	0.418535	3.4:+100347100	MS.gene68113:intron
AGAGAATATTGTGACTGATT+TGG	0.419588	3.4:+100352295	MS.gene68113:CDS
TTCCGTCTTCTGCACCTGAT+GGG	0.421600	3.4:+100343257	MS.gene68113:CDS
GACTTGCGTGATATTGGATA+TGG	0.432369	3.4:+100346350	MS.gene68113:CDS
ACCTCTCCAAGATCAGAATC+AGG	0.444389	3.4:-100347316	None:intergenic
TCACTTCAGATCCGTCTCTC+TGG	0.446265	3.4:-100352318	None:intergenic
AATGTTCCCCTCTTGCTACT+AGG	0.449463	3.4:+100346596	MS.gene68113:CDS
TCTTTCTGTCAAACTTATGC+TGG	0.451415	3.4:+100343583	MS.gene68113:CDS
TCTTTATCTGGAGACAGGTT+GGG	0.453512	3.4:+100346515	MS.gene68113:CDS
GCAACATGAAGGGTATAATC+TGG	0.458383	3.4:-100347175	None:intergenic
GGGAATTATTATTATGGCCA+AGG	0.467920	3.4:+100343319	MS.gene68113:CDS
TGAAGATAGAGATGAGCATT+GGG	0.469243	3.4:+100348049	MS.gene68113:CDS
CTTTCCTGAAATTGAACACT+GGG	0.475024	3.4:-100347289	None:intergenic
CTCTTTATCTGGAGACAGGT+TGG	0.477261	3.4:+100346514	MS.gene68113:CDS
TATTTCTATGATCCTGAGGT+TGG	0.479248	3.4:+100343298	MS.gene68113:CDS
ATTTCTATGATCCTGAGGTT+GGG	0.481090	3.4:+100343299	MS.gene68113:CDS
TTCTGTCAAACTTATGCTGG+AGG	0.481239	3.4:+100343586	MS.gene68113:CDS
CATAATCATCAGCATGAAAA+CGG	0.481588	3.4:-100343456	None:intergenic
CGTGATATTGGATATGGAAA+GGG	0.488820	3.4:+100346356	MS.gene68113:CDS
TAATAATAATTCCCAACCTC+AGG	0.489340	3.4:-100343310	None:intergenic
CACTGGAGAACAACTGCACC+GGG	0.492326	3.4:-100346482	None:intergenic
GATATTATCAAGTAAATGTC+GGG	0.495895	3.4:-100347224	None:intergenic
TCACGCAAGTCGCCAGTACC+AGG	0.497831	3.4:-100346338	None:intergenic
ACCTTTAATCGGCAAAGTAA+TGG	0.504079	3.4:+100346448	MS.gene68113:CDS
ATGAAGATAGAGATGAGCAT+TGG	0.505454	3.4:+100348048	MS.gene68113:CDS
CAGACTCTTTATCTGGAGAC+AGG	0.508402	3.4:+100346510	MS.gene68113:CDS
CATAACCCCTGAAACACAAC+AGG	0.512836	3.4:+100343495	MS.gene68113:CDS
TCCCTGTCTCTAGCAGGAAA+AGG	0.513158	3.4:-100343427	None:intergenic
GTGGTGGCGGTTACACCATT+CGG	0.513823	3.4:+100346618	MS.gene68113:CDS
TAAGAAGTTCTAAGATTGCA+AGG	0.514247	3.4:-100343732	None:intergenic
TATTCATAATACTCGTGCTC+TGG	0.514478	3.4:-100347145	None:intergenic
TTCGGAATGTTGCTCGGTGT+TGG	0.515145	3.4:+100346636	MS.gene68113:CDS
CAATCCATAGTGGGCAAGAA+GGG	0.516417	3.4:-100343380	None:intergenic
ACTTATGCTGGAGGTTCAGT+TGG	0.516719	3.4:+100343595	MS.gene68113:CDS
CAAAGGTCCCTGTCTCTAGC+AGG	0.518536	3.4:-100343433	None:intergenic
TATGCTGGAGGTTCAGTTGG+TGG	0.521919	3.4:+100343598	MS.gene68113:CDS
CGGATACGATGTGGCTTCAT+GGG	0.525525	3.4:-100343346	None:intergenic
TGTTACTTGGGGCAACATGA+AGG	0.526854	3.4:-100347186	None:intergenic
GTTGTAGCGTGTTCTATATG+TGG	0.527612	3.4:+100346220	MS.gene68113:intron
TAGAGAGCACTCAAGGGTCT+CGG	0.527622	3.4:+100352062	MS.gene68113:CDS
TGAATCTCAGCTCAATGATC+CGG	0.531268	3.4:+100348224	MS.gene68113:CDS
GAGTATAGGCCATCAAAGAC+AGG	0.531330	3.4:-100343562	None:intergenic
GATATTGCTGTTAACTGGGC+TGG	0.533945	3.4:+100343649	MS.gene68113:CDS
GACTGATTTGGCCAGAGAGA+CGG	0.534839	3.4:+100352307	MS.gene68113:CDS
GCAATCCATAGTGGGCAAGA+AGG	0.536235	3.4:-100343381	None:intergenic
AAAGAACTAGAGAGCACTCA+AGG	0.537212	3.4:+100352055	MS.gene68113:CDS
TGCTTTAATCTTTCAATTAG+GGG	0.539074	3.4:+100346539	MS.gene68113:CDS
CCCCTCTTGCTACTAGGTGG+TGG	0.539586	3.4:+100346602	MS.gene68113:CDS
ACACTGGAGAACAACTGCAC+CGG	0.541503	3.4:-100346483	None:intergenic
CCTTTCCTGAAATTGAACAC+TGG	0.543136	3.4:-100347290	None:intergenic
ACACCATTCGGAATGTTGCT+CGG	0.543584	3.4:+100346630	MS.gene68113:CDS
ATGATCCTGTTGTGTTTCAG+GGG	0.546511	3.4:-100343500	None:intergenic
GCGTGATATTGGATATGGAA+AGG	0.546993	3.4:+100346355	MS.gene68113:CDS
GGATATTATCAAGTAAATGT+CGG	0.550643	3.4:-100347225	None:intergenic
CTCTAGCAGGAAAAGGCTTG+TGG	0.551357	3.4:-100343420	None:intergenic
CTTAATGTTCCACTTGATGA+CGG	0.553928	3.4:+100346392	MS.gene68113:CDS
AAGTTTGACAGAAAGAGTAT+AGG	0.555370	3.4:-100343576	None:intergenic
CCAGTGTTCAATTTCAGGAA+AGG	0.561391	3.4:+100347290	MS.gene68113:CDS
CAACTCAAACGCTTTAACGT+TGG	0.566804	3.4:+100343529	MS.gene68113:CDS
TCATCAATGCCGTCATCAAG+TGG	0.566825	3.4:-100346401	None:intergenic
ATTGATATCCACCATGGAGA+TGG	0.567849	3.4:+100346245	MS.gene68113:CDS
TCATGTTGCCCCAAGTAACA+TGG	0.568928	3.4:+100347189	MS.gene68113:CDS
TGGATTGCTTCAGCACATGC+AGG	0.569680	3.4:+100343396	MS.gene68113:CDS
CTTCCTCCACACCATCTCCA+TGG	0.571898	3.4:-100346256	None:intergenic
CTCTTGCTACTAGGTGGTGG+CGG	0.573617	3.4:+100346605	MS.gene68113:CDS
GTTACTTGGGGCAACATGAA+GGG	0.581802	3.4:-100347185	None:intergenic
ATGTGATATTGCTGTTAACT+GGG	0.581883	3.4:+100343645	MS.gene68113:CDS
GATTCTCAAGTAGCTTAGAT+CGG	0.588060	3.4:-100347246	None:intergenic
TCCGTCTTCTGCACCTGATG+GGG	0.590771	3.4:+100343258	MS.gene68113:CDS
ATTGCTGTTAACTGGGCTGG+TGG	0.595034	3.4:+100343652	MS.gene68113:CDS
TGCTCGGTGTTGGTGCTATG+AGG	0.602036	3.4:+100346646	MS.gene68113:CDS
ACCCCATCAGGTGCAGAAGA+CGG	0.603020	3.4:-100343259	None:intergenic
ATGCTATTTCTATGATCCTG+AGG	0.612816	3.4:+100343294	MS.gene68113:CDS
TGTGCTGAAGCAATCCATAG+TGG	0.612825	3.4:-100343390	None:intergenic
GTGCTGAAGCAATCCATAGT+GGG	0.613771	3.4:-100343389	None:intergenic
ATAAAGAGTCTGCACCACAC+TGG	0.616250	3.4:-100346499	None:intergenic
GTAATGGAAATCTTTAGGCC+CGG	0.623225	3.4:+100346464	MS.gene68113:CDS
TCCTGATTCTGATCTTGGAG+AGG	0.628534	3.4:+100347315	MS.gene68113:CDS
CGCCACCACCTAGTAGCAAG+AGG	0.632522	3.4:-100346604	None:intergenic
GTTCCCCTCTTGCTACTAGG+TGG	0.633802	3.4:+100346599	MS.gene68113:CDS
GCCACCACCTAGTAGCAAGA+GGG	0.635670	3.4:-100346603	None:intergenic
GTGGCTTCATGGGATGACCT+TGG	0.639461	3.4:-100343336	None:intergenic
AGAACTTCTTAAACAGCACG+AGG	0.642387	3.4:+100343744	MS.gene68113:CDS
TTGGGATCCAGATTCTGGCA+TGG	0.646557	3.4:+100348067	MS.gene68113:CDS
CAAGAAGGGCATGTGTCATG+CGG	0.648533	3.4:-100343366	None:intergenic
CATCAGCATGAAAACGGCAA+AGG	0.654492	3.4:-100343450	None:intergenic
ACACCGAGCAACATTCCGAA+TGG	0.663792	3.4:-100346633	None:intergenic
AAGAACTAGAGAGCACTCAA+GGG	0.664552	3.4:+100352056	MS.gene68113:CDS
TATCCACCATGGAGATGGTG+TGG	0.668224	3.4:+100346250	MS.gene68113:CDS
CCACCATGGAGATGGTGTGG+AGG	0.672038	3.4:+100346253	MS.gene68113:CDS
CCACCACCTAGTAGCAAGAG+GGG	0.675201	3.4:-100346602	None:intergenic
GCATGGACATTGATGTTGAA+CGG	0.679554	3.4:+100348084	MS.gene68113:CDS
GTGGACATTGATATCCACCA+TGG	0.680210	3.4:+100346239	MS.gene68113:CDS
CCTCCACACCATCTCCATGG+TGG	0.680490	3.4:-100346253	None:intergenic
GCTTCACACATGGATGTCAG+TGG	0.682917	3.4:+100343226	None:intergenic
GCATCATGCCAAGAAGTGTG+AGG	0.688961	3.4:+100343678	MS.gene68113:CDS
GGTGCAGTTGTTCTCCAGTG+TGG	0.689736	3.4:+100346485	MS.gene68113:CDS
TGTGTCATGCGGATACGATG+TGG	0.693446	3.4:-100343355	None:intergenic
TCTCCAAGATCAGAATCAGG+AGG	0.695400	3.4:-100347313	None:intergenic
TGTAGTTGAAACAAGCGTGA+AGG	0.725827	3.4:+100352092	MS.gene68113:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content

Chromosome	Type	Strat	End	Strand	Name
chr3.4	gene	100343235	100352340	100343235	ID=MS.gene68113
chr3.4	mRNA	100343235	100352340	100343235	ID=MS.gene68113.t1;Parent=MS.gene68113
chr3.4	exon	100343235	100343765	100343235	ID=MS.gene68113.t1.exon1;Parent=MS.gene68113.t1
chr3.4	CDS	100343235	100343765	100343235	ID=cds.MS.gene68113.t1;Parent=MS.gene68113.t1
chr3.4	exon	100346227	100346667	100346227	ID=MS.gene68113.t1.exon2;Parent=MS.gene68113.t1
chr3.4	CDS	100346227	100346667	100346227	ID=cds.MS.gene68113.t1;Parent=MS.gene68113.t1
chr3.4	exon	100347106	100347336	100347106	ID=MS.gene68113.t1.exon3;Parent=MS.gene68113.t1
chr3.4	CDS	100347106	100347336	100347106	ID=cds.MS.gene68113.t1;Parent=MS.gene68113.t1
chr3.4	exon	100348035	100348105	100348035	ID=MS.gene68113.t1.exon4;Parent=MS.gene68113.t1
chr3.4	CDS	100348035	100348105	100348035	ID=cds.MS.gene68113.t1;Parent=MS.gene68113.t1
chr3.4	exon	100348188	100348245	100348188	ID=MS.gene68113.t1.exon5;Parent=MS.gene68113.t1
chr3.4	CDS	100348188	100348245	100348188	ID=cds.MS.gene68113.t1;Parent=MS.gene68113.t1
chr3.4	exon	100352045	100352113	100352045	ID=MS.gene68113.t1.exon6;Parent=MS.gene68113.t1
chr3.4	CDS	100352045	100352113	100352045	ID=cds.MS.gene68113.t1;Parent=MS.gene68113.t1
chr3.4	exon	100352245	100352340	100352245	ID=MS.gene68113.t1.exon7;Parent=MS.gene68113.t1
chr3.4	CDS	100352245	100352340	100352245	ID=cds.MS.gene68113.t1;Parent=MS.gene68113.t1

Gene Sequence

>MS.gene68113

ATGGATGTCAGTGGCAATTCTCTTCCGTCTTCTGCACCTGATGGGGTAAAGAGAAAAGTATGCTATTTCTATGATCCTGAGGTTGGGAATTATTATTATGGCCAAGGTCATCCCATGAAGCCACATCGTATCCGCATGACACATGCCCTTCTTGCCCACTATGGATTGCTTCAGCACATGCAGGTCCACAAGCCTTTTCCTGCTAGAGACAGGGACCTTTGCCGTTTTCATGCTGATGATTATGTTTCATTTCTTCGAAGCATAACCCCTGAAACACAACAGGATCATATGAGACAACTCAAACGCTTTAACGTTGGTGAAGATTGTCCTGTCTTTGATGGCCTATACTCTTTCTGTCAAACTTATGCTGGAGGTTCAGTTGGTGGTGCTGTCAAGTTGAATCACGATCAATGTGATATTGCTGTTAACTGGGCTGGTGGACTGCATCATGCCAAGAAGTGTGAGGCTTCTGGATTTTGCTATGTTAATGATATAGTCCTTGCAATCTTAGAACTTCTTAAACAGCACGAGGTTTGTCTAGCCGTGGAATTCCCTCTGCTTTAATTTTTCTTCATACTTTAAAGGTATATTTCGTGGAATCGATTGGATTTTTTATATTTCAATTTTCTTTAGAACTGATTTATGGTAAGTACTGAAGTTAATATTTAGGAAGTGTAGCTCAGACTTGCATGCTATATACGAGGATAAAAGAGTATTTCTCTCCTTGATCTTATTTTCTGTTAGATCCTTCTGGAAACTCATTCACAGGGACAACACTAAATTTAATGAAACACGAAAAAAATGTTACTTTTCATCTGTGACGCTTTTGCTGTAAACTCACTCAGTGTCTATTATCAATCAGAACATGAAGCCATAGTCAGTATATTTTACAACACTAATGTTGGTCAGGTTTGCATCCACACATTTCTTTTCTCCTAACAAGGCACTTATGTCTTCCTCAGTACATCTAAATAAAAGTAAGCTTGAGCACACCATTGTCCGTTAACTAGTCAGTTTAGTGTTCAATATAAAGGAAAATGCTATCTATATTCTATAGTATTTGACAACATGGCACGCTTTCTGGTTTCTAGGAGAATGTTCAGCTGGGTAAAGGTTTTCATAATTTTATCATGCACTTAGAGTCAACTAGCAGTTCTTGTTACATATAAGTTATAACTATAAGTTTGGTTAATTATTTTTCCGTATGTCCTTTCTGATATGTATTCTTTAAATCTCACATAGGGGTTAGGCTGTTCACATCAAGAGTGCTTTACCTATTGATGCTTTGTGCATGACCAGAATAATGTTAGTATTTAGTTGGTTTGGTTTGTACCAATTTGAACTGTTAGAGTTAATCGGCCATTTGTTTCTCAACTCAAATATTTGTACATTAATGTGGTGGTATTTTTCTCCAGTTATACTACAGTTTTCTCTTGTTTGCTCTGGTTTCAACAATAAAATTTTGTTCTCCTAGGATAGTCTTCCATAATATATGTGCTGCAATGATATATTACATAAGTTCTAAGCAATCATGTCAATGGTGGATGGTGCAATATGGTAGCCAGTAAAAAATCTGACATATATCATGTGCGACAGCAATGCGGCTATGAAGGAAGCATGGTGGATAGAGTAAAAATATCACATACACACCAAAAGAACTGCAGAAAAGAATTAAAAAAATGACAATATTAAATAACAATTAAAACTCAATTGATGGAAAATATTTTAAAGTCACATAATCCTATATCATAACCTCATCACCATCAAGGTCAAGACCTCAAATAACAACAAAATGATAAAAACAATACAAACAAAAAGTAAACGAGAACAATGAAAAAACAGAATGAAAAATAGACAGTAAGGCAGAGGCGAAAAAAGGGACTGAAGAAGAATGAACAAAATTAGAGAATGAGATGGATATAGCAGAATCCAGGAGATAAAAAAAACAGAAGCAGGAAGAACAGAGGAGGTCGCCAGAACTGGGTGGTTGCTGTATGCCATAATACAGACAGCCAGTCACTAGAGATAGAAACGATGAACAACGAGATGAATGAGAGTGTAGAGTGCAACAGTGCATTGAAGAGAACTGCTGCAAATGCGATGCTGCAACTTTCTTCTAGGGTTTTCCATCACGGGCCTAACTTTTCATGGCCAGGTTCCGTTTTTTTTTTTTTTTTTTTTTAATTTTGAATTTATAATATTTTTGGTGGATATTTTTGCCATGGCTGCCATTGCCCATTGGTGCCTGCTGCGCCCAGTGAATGGTGGCTGCTATGGCCCTCCATGAAGAACCCTCCACTCTACGGTGGAAATTACAACACTGGTTCTATGTGTAGGTAGCTGCATGCATTTAAGTTTTGAACTAAATTTACTGCTTTTAAAGAAAACATACTTTGACGGAGAAACGACCGTGAATTTTCCAAACTATTTAGTTTCAATACATGCTTCTGTTGGAATTTAACCATTATAAGTGCTGCCTCAATTTGGTATCATACTGTTTCAAAAAGAAATAATGGAGAGCCATTTGAATACTACGATTTTGTTTGTGCAATAAGTCACCAACATCTGCGACTGCAGTACTGCCTACTCATATTTAGTAGATATAAAATAATTAGAGGATTGCTGTCTTATCGTGTTAATTATACAATGGAACGTCTAGTTAAGCCTGCTCTTAAAGCAGTAGGTAAAATGAATTGCATAGTTTAGTTTATATGGGACACACAACAAGCCAAAAACCATTTTGAGGTTTGTTATGGATCGAAAAATTCCTCGACTATGGCATAGGTTTTACCGCTTGATGATTATGCTCATATTTGTTCAAAATATTAAGCTGTAGTCCCTGAAATATTCAGAAACAATACGAGGGCATAAACTGTAGAAAGTTTGTTTGGCAGGTGACAGTGACAGTGCTAATGTTTTGAACTTCTCAAGTGTGTTTGCATACTGCTGTTTCAGTTCATATTTTAAAATGCTAATGTTACGTTTGTTGTAGCGTGTTCTATATGTGGACATTGATATCCACCATGGAGATGGTGTGGAGGAAGCTTTCTACACTACTGATAGAGTCATGACTGTTTCTTTCCATAAATTTGGTGATTATTTTCCTGGTACTGGCGACTTGCGTGATATTGGATATGGAAAGGGAAAATACTACTCTCTTAATGTTCCACTTGATGACGGCATTGATGATGAGAGTTATCATTTGTTGTTCAAACCTTTAATCGGCAAAGTAATGGAAATCTTTAGGCCCGGTGCAGTTGTTCTCCAGTGTGGTGCAGACTCTTTATCTGGAGACAGGTTGGGATGCTTTAATCTTTCAATTAGGGGTCATGCAGAGTGTGTCAGATACATGAGATCATTCAATGTTCCCCTCTTGCTACTAGGTGGTGGCGGTTACACCATTCGGAATGTTGCTCGGTGTTGGTGCTATGAGGTAAAACATTGTTTTTCCTTTATTTGATGTAATATCACTAATATAAGGTAGCTTGATTTTATTTATAAGGCAACCAGAGACCTCTGTCAGAGTGCCAAAACATCTAGTTGAATTATAATATTGAAATTTCAATATTTTCTACATATTTCATGTTAATTATAAAAAATGCTAAACATGAACTAATTCATTATGTAAAACAAGCGATGGTGTTCAAGAAGTCTAAGCAGCTGTGTATTGTTTCCTTATACTAGTCTCGAACTATAAAATATAAATTGAGATATTATTAAAGCCTCTCAGAATGCAATGTGACATTTCATGTAATTATTTCTCATATCTCAGTTTGTTCAGTGTTTTAATATTTATTTACAATTTTAGTCAATCTTCTGTCTAGACTTTCATCCAAGTACATAAAATTTTATTGGCTATTTGTTAAGGCAGACGGGAGTTGCTCTTGGAATAGAAGTTGATGACAAAATGCCAGAGCACGAGTATTATGAATATTTCGGTCCAGATTATACCCTTCATGTTGCCCCAAGTAACATGGAAAACAAAAATACCCGACATTTACTTGATAATATCCGATCTAAGCTACTTGAGAATCTTTCGAAGCTGCAACATGCTCCCAGTGTTCAATTTCAGGAAAGGCCTCCTGATTCTGATCTTGGAGAGGTAAATTATTTAACTAAATTTTTCCCCGTTATCTTTATTTCACGCATGTTTGTTTTCAGCAGAAGATAAGAGCCTATTTTGTGCAAATACTTTCTGTTCTTGTTTGCTTTGTTTCCTGTTTACAAAATAACTTCTGTTTTCGTTGACTTTTATACAAGTATTTGAAAACAGGAAACCAAGTAATACAAGTGAAATTAAGAAATAAAACAGAATATGTTTGCAAAGCAAACTAGCCCTATAAAATTATTTTTGAACACACTGATGCATAAGGTACCCACTACCCACATATAGGGATTCAAAAAAGAATTTTAATTTTAACTGTATTATCCCATGCAAAAGGAGCAACTTGAATACTTCACAGGAAAAAAGCATTTCTAACCAGATCCATGACTCCTTCAGAGAATAATTCTGTGTCAATAGTCCTATACTCAGGTGCAATGCGATTAACAGCTCTTCTCTTACCTTCCTTACTCTTAAATTCCCCTCCTTAATAGAATACCCCACTTCTTCTACTACACTACTGGACACATCATTAACTATCCTTTGGCCCCACCTTCTTCATCCTAGAATATATGATAGGAGCCAAAATTAAATAGAAGAACTGAATGGTATTTATACTGACTTTTCCCTAACTTGTCCTAGCTATTTTTGACTCTCTTCATCCTGTTTGAACTCATTCCCTATTCACAATTCTCCAGGAATTTGACGATCATGAAGATAGAGATGAGCATTGGGATCCAGATTCTGGCATGGACATTGATGTTGAACGGTATTTCACTTTATAACTGTATACTAAATTTACTTATCATCAAGTATCACATGATCAGTGTGTCCTTTACCTCATTTATCAGCGATATTCTCCAAAGCAAAGTCAAGAGAGAAATTGTTGAATCTCAGCTCAATGATCCGGTGTGTGTATTTATCTTTTGAAGTATTTAATTTAAATATATTAATTTCTTGTGGAGAATCTAATATTTGTAGAACATATGACGTGTGTTGTTAGGAATTGTATCTAAACCTACATGCATTTACAACATTTTCCGACAAATATCTAGAATTAAATTTGATATACTCTTAGTTACTTTAATGTGGCAACCATAGTATTTACCTGTTAACAAATCAACAGTCCATTCCTTTGTCAGTGCACTGTTCAAAACCCCACGTACTTTTTTTACAGTTCTCAGTCCCCATTTAATTTTGTTCATTTTTAATCTTGTACAACCTACTGTTAGAGAGAAGGGAAAGGGGAAAACCTTGTCAGTCGAGAGAAGAAATTCTTTTACCCTTTAAGCCTTAGCAAATATGCATTAACTATTAATTTTCGAGTGGATTTAAAAGCCACAAAATACTGGCTATTTCTTAAAGAAAATAGAACTGATTAGTTTATCTCCAAAGAAGTTTGGGAATTTGTCATATACCTGCATGTGGGATCGAGTCTATGGGGAAAGTAAGAAGCAAGACTTGTCGTTGACTAACCTAAGTTTTCTCAAAATGATTTCATTCTTGTTTATTCCAATTCCAATAGCAGCTTGTCTTTTTAGAGGTTACAGATAATAAAGAGAATCCTAACCAAACTCTACCACCAATAAGGATTATGTTTTGCAAAAGTACATTTAAGGCTTCTTATTTTAAAAAGATTAAATAGTTCAAATCTTTTTTATCCATATGATTGCTCTCCTCTTCGATCTTGAGCTTGTCCTTAAGCTTAAACTTTGGATAAGCTTCTTAGAAATGTGTTAGACAACCTTCTGAACCACTTCTGTCATCAGAAACCTCCTCTAGTGTCATCGATTCAAAAAGTGTGTCAAGACCAAGCTCAAATATGGGAAACACTAACGTTGGATCTTTGCACGTGCGAACTCTAGCAAATCGCCTTTTGTCAATGAAAGACAGCCCCAAACAGGGGAGGAGCTACACTATGTTGAGGGTGTTCAAGTGAACCCCCTGAACCGGAAAAAAAAACCTGTAAATCCAATATATTTTTACTTTTTGAACCGCTCAAATATTGTTCTTGCACCTTAACTCAAAGAACCCAAGAGAAGAAACAACACGGGAGATGGTAAATAGAGGATATGCAGGCAATGCAAAAATACCATTGCGTAGATGCAAGCATGAAAAAGGAAGTGTGAGCGGCGGCCAGTTAACAAGAAGACTATTTAGGGTTTTCTATAGTCCACTTTGGGCTTTTCACTATGTGGCCTATTTCTTTTTTACCAAAATGTGGCCCGCGTGTTATATTAGAGTATCTGATAGTTAAGCTTAGTCTAGTTTGTTTTATATCTAAGCTTAGCATAAGCCAGCTGTACACAGCTGGCATCTCTTATTCATTGTAACTGGCAAGTGTAAGGAGTCTTACACTTGACCTGCTATTGCAGGTTTCCTCAATCCATCTATAAAAGATGGAATGTAACAGAAATATTTCATTCAATGAAAAATCAGAATTCAGTTCAAAAGTTAGTTAGATCTATTAGATATTTCTCTCTCTACTCTACCAGATTTTATATGTTATGTCTAGTTGTGTTTCTTTTATTTAACCGAAGAAAACAATGCGTGTTGTCCCCGTGAGCATAGCTCAACTGGTAGGGACAATGCATAATATATGTTAGGGCTGGGGTTCAAACCTCATACACCCATGAGCTCCAGCCACTAGGCTACTTGACCAAAAAAATAAATAATGTGTGTTTAATGGCATTTAAAAAACATATAAAAAGATAAAATATTGCCGGATAAGGCAGAAATTGTTAAACTAATTGATTGAATCATTTAGACTTATAAAAGAATGATTTATTTAGTCTTCACAATATATATTTTTGGCTTAATTGCACGTTTGATCCCTTATGTTTATTTTAGTTTTTAAGTTGGTCCCTTATGTTTTAAAAATTTCAAGTTGGTCCCCTTATGTTTGAAAAGTTTCAAGTTGGTCCAAAACGTTACTCACATTGGAATAAGTTAGTCCATTCCACGACATGATATTGAGTTTAATAAAACAAAAAAAAAATTAAAAAAATGTTTTAAAACTGATTTTCTAAATAAGTTAAGTTCCATCACCCATATTGTCAACCCATATCTTTAAACGTTAAGTGATTGAAGGGACTAACTTATTCCAACGTGAGTAATGTTTTGGACCAACTTGAAACTTTTAAAACATAAGGGACCAACTTGAAACTTTTAAAACATAAGGGACCAACTTGAAAACTAAAATAAACATAAGGGATCAAACATGCAATTAAGCCTATATTTTTTATATTCGGTTTCACTGCTATAAACTATTAAATTTGTAAAGATTATATATGTAATTTTTTTTTTTGCACACATACATGTAGGGATGATAATGAGTATCTATGGGTAAAATAATATGATATTTGTGCTCTTTCCGTTGAATAAATCTAACTCGTGTTTACTCCATATCCTTGTGATTATCCGTTAAAAGGATAATCAACAAATTTAGAGGTACGAGATTATGAACATTCTAATTTCATTGTTGTTTTTGTTGTCGTAGGAGATTATGAATAATTTCTTTATGAGAAAAATATCGTAGTATGAGCATGAATTTATTTATCAACATCATATTCATGAAATTATTGGTGTTTTTTTTTTTTTTTTGAAAAAAAAAAATTTGAACCCCCTAGGCCAGGGTCCTGGCTACGCCAATGGCTCCAAACCATCATCTAGTTGAATGAAAAGTTTGCAAGGCCATTTGTCCTCATCCCTTACATTTTACAGCAGACATAATATACTTTCTTACTGCGACACCCTTAATGTATAATATACCAGTCATGCCTAATTGGAAGGAAGGAAAGGGATGTGAATAAAGTTGAATCCGCGTATTTTTGCCTTTCAGGCAAGCTGCGAAGCTTTTCTTGCTGACGACGAAAGCTTCAAACTCAGACCGAGAGACACCTTACTTTGTAACACCTAACTGTTGAAAAACATGCAAGCCAAGTTTGACAGATTGTGCAAGTCTTTACAAAGATTTGGACAAACGACAAACGTATAAAGGACTTATCCTAAGGAAGAAATCTAGGAGATTGCTCCATATAACTGCTTAAGTTAGATCACCATGAAGGAAAAACCATTTTAAACACCCAACTAGCGAAGAGGTCAATCATAGATATCTGCCATAGACAAAAATAGGCTAATAGGACATTTTAGTCACGAGAGACTCTACCATCAGAAACCAAACACCCTAGAATATTATTTGGCCACCATTTTAGCCTTGAGACGACCCAGGTTAGCATTAGGTCGACCCTGATGGTAAAGATCCACCAATAACTTGAATGGAGAAAATGTACAAGGTTCCAAGTACCGCTCTTCTGAAGAGCACACATTTCATCAATAGTTCAAATACACTGATAAAATTGTATTTAGTGTGTTGCTGCCTATCAAGGAATAGGAATGAAGATGGAGGAAAATCTCAACAAAGGCTGAGATTCCCATGTATCACACTTTGTATTATTTGAGAACTGTTTTGACATTAGAGTCCAAAGACACAACTATAGAGGCTCTGGCAGCAGCTGATGAGTGATTCTTCTTTTTTTTTTTTTTTGAGCAAAGATGAGTAATTCCTCTATGAATGAATTGCATTGTTGTGCAATCCTCAGGCAGCTAATATATTTTATGCCTCTGTTGAAAATTATTTATATTAATTTACAAACAAACTCATGGTATGGAAAATTATACAATTTGTAATGCTGGCTTATCATGCCAGGAGGACCTGAAAAGAACTAGAGAGCACTCAAGGGTCTCGGATACTGCTGTAGTTGAAACAAGCGTGAAGGTGAGTTTCATAATTTCATAAAAAGTACAGATGCATAAAAATTAAATGTATGCATGCATAAGTATCAGTGCATTTTATTGAAAATATGTGGTTGCTATAAAACTTCTACTTATTGAATTTGGTTCTTGCAGGCTTTAGATATTTGTTCTCAGCCAGTTGATGAAGATAATGTGAAAGATGAAGAGAATATTGTGACTGATTTGGCCAGAGAGACGGATCTGAAGTGA

Protein sequence

>MS.gene68113.t1

MDVSGNSLPSSAPDGVKRKVCYFYDPEVGNYYYGQGHPMKPHRIRMTHALLAHYGLLQHMQVHKPFPARDRDLCRFHADDYVSFLRSITPETQQDHMRQLKRFNVGEDCPVFDGLYSFCQTYAGGSVGGAVKLNHDQCDIAVNWAGGLHHAKKCEASGFCYVNDIVLAILELLKQHERVLYVDIDIHHGDGVEEAFYTTDRVMTVSFHKFGDYFPGTGDLRDIGYGKGKYYSLNVPLDDGIDDESYHLLFKPLIGKVMEIFRPGAVVLQCGADSLSGDRLGCFNLSIRGHAECVRYMRSFNVPLLLLGGGGYTIRNVARCWCYETGVALGIEVDDKMPEHEYYEYFGPDYTLHVAPSNMENKNTRHLLDNIRSKLLENLSKLQHAPSVQFQERPPDSDLGEEFDDHEDRDEHWDPDSGMDIDVERDILQSKVKREIVESQLNDPEDLKRTREHSRVSDTAVVETSVKALDICSQPVDEDNVKDEENIVTDLARETDLK