AlfalfaGEDB | MS.gene71949

M. sativa cultivar XinJiangDaYe / MS.gene71949

KEGG: K02983
GO: GO:0003674 GO:0003735 GO:0005198 GO:0005575 GO:0005840 GO:0006412 GO:0006518 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0019538 GO:0034641 GO:0034645 GO:0043043 GO:0043170 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0071704 GO:0110165 GO:1901564 GO:1901566 GO:1901576
PFAM: PF04758.17

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene71949.t1	XP_003608708.1	100	62	0	0	1	62	1	62	4.10E-25	123.2

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene71949.t1	P49689	95.2	62	3	0	1	62	1	62	2.4e-26	118.6

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene71949.t1	G7JW33	100.0	62	0	0	1	62	1	62	3.0e-25	123.2

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049159	MS.gene71949	0.801412	9.12E-49	-1.69E-46
MS.gene049255	MS.gene71949	0.869839	2.29E-66	-1.69E-46
MS.gene049340	MS.gene71949	0.812269	4.62E-51	-1.69E-46
MS.gene049341	MS.gene71949	0.839594	1.44E-57	-1.69E-46
MS.gene049501	MS.gene71949	0.80238	5.77E-49	-1.69E-46
MS.gene049821	MS.gene71949	0.858395	8.48E-63	-1.69E-46
MS.gene049825	MS.gene71949	0.825554	4.40E-54	-1.69E-46
MS.gene049873	MS.gene71949	0.833048	6.67E-56	-1.69E-46
MS.gene050020	MS.gene71949	0.80359	3.24E-49	-1.69E-46
MS.gene050277	MS.gene71949	0.885445	8.08E-72	-1.69E-46
MS.gene050303	MS.gene71949	0.875185	3.76E-68	-1.69E-46
MS.gene050689	MS.gene71949	0.801382	9.24E-49	-1.69E-46
MS.gene050721	MS.gene71949	0.802913	4.48E-49	-1.69E-46
MS.gene050807	MS.gene71949	0.823628	1.25E-53	-1.69E-46
MS.gene050808	MS.gene71949	0.801092	1.06E-48	-1.69E-46
MS.gene050815	MS.gene71949	0.843439	1.40E-58	-1.69E-46
MS.gene050838	MS.gene71949	0.809859	1.54E-50	-1.69E-46
MS.gene050916	MS.gene71949	0.836136	1.12E-56	-1.69E-46
MS.gene051241	MS.gene71949	0.848779	4.93E-60	-1.69E-46
MS.gene051243	MS.gene71949	0.84071	7.38E-58	-1.69E-46
MS.gene051247	MS.gene71949	0.821932	3.10E-53	-1.69E-46
MS.gene051328	MS.gene71949	0.882171	1.30E-70	-1.69E-46
MS.gene051330	MS.gene71949	0.865576	5.34E-65	-1.69E-46
MS.gene05134	MS.gene71949	0.890704	7.74E-74	-1.69E-46
MS.gene051451	MS.gene71949	0.831558	1.56E-55	-1.69E-46
MS.gene051633	MS.gene71949	0.826812	2.21E-54	-1.69E-46
MS.gene051700	MS.gene71949	0.842998	1.84E-58	-1.69E-46
MS.gene051746	MS.gene71949	0.818181	2.24E-52	-1.69E-46
MS.gene051748	MS.gene71949	0.811834	5.74E-51	-1.69E-46
MS.gene05188	MS.gene71949	0.856279	3.58E-62	-1.69E-46
MS.gene051903	MS.gene71949	0.854309	1.34E-61	-1.69E-46
MS.gene051975	MS.gene71949	0.832816	7.62E-56	-1.69E-46
MS.gene052012	MS.gene71949	0.813298	2.75E-51	-1.69E-46
MS.gene052133	MS.gene71949	0.82699	2.00E-54	-1.69E-46
MS.gene052286	MS.gene71949	0.8137	2.24E-51	-1.69E-46
MS.gene052302	MS.gene71949	0.82324	1.54E-53	-1.69E-46
MS.gene052376	MS.gene71949	0.840728	7.30E-58	-1.69E-46
MS.gene052519	MS.gene71949	0.813488	2.50E-51	-1.69E-46
MS.gene052879	MS.gene71949	0.811044	8.52E-51	-1.69E-46
MS.gene052947	MS.gene71949	0.901319	3.02E-78	-1.69E-46
MS.gene053008	MS.gene71949	0.819054	1.42E-52	-1.69E-46
MS.gene053080	MS.gene71949	0.81081	9.58E-51	-1.69E-46
MS.gene053122	MS.gene71949	0.813512	2.47E-51	-1.69E-46
MS.gene053226	MS.gene71949	0.844921	5.60E-59	-1.69E-46
MS.gene053230	MS.gene71949	0.814678	1.36E-51	-1.69E-46
MS.gene05364	MS.gene71949	0.815695	8.10E-52	-1.69E-46
MS.gene054657	MS.gene71949	0.814689	1.36E-51	-1.69E-46
MS.gene05476	MS.gene71949	0.833156	6.27E-56	-1.69E-46
MS.gene055162	MS.gene71949	0.809435	1.90E-50	-1.69E-46
MS.gene05517	MS.gene71949	0.829444	5.13E-55	-1.69E-46
MS.gene055466	MS.gene71949	0.818196	2.22E-52	-1.69E-46
MS.gene055468	MS.gene71949	0.818854	1.58E-52	-1.69E-46
MS.gene055512	MS.gene71949	0.815368	9.58E-52	-1.69E-46
MS.gene055555	MS.gene71949	0.82498	6.01E-54	-1.69E-46
MS.gene055580	MS.gene71949	-0.826349	2.85E-54	-1.69E-46
MS.gene055608	MS.gene71949	0.815714	8.02E-52	-1.69E-46
MS.gene055623	MS.gene71949	0.824225	9.06E-54	-1.69E-46
MS.gene055778	MS.gene71949	0.806933	6.47E-50	-1.69E-46
MS.gene055808	MS.gene71949	0.881603	2.09E-70	-1.69E-46
MS.gene056043	MS.gene71949	-0.824194	9.21E-54	-1.69E-46
MS.gene056091	MS.gene71949	0.852913	3.38E-61	-1.69E-46
MS.gene056215	MS.gene71949	0.844783	6.10E-59	-1.69E-46
MS.gene056580	MS.gene71949	0.809201	2.13E-50	-1.69E-46
MS.gene056601	MS.gene71949	0.815785	7.74E-52	-1.69E-46
MS.gene056631	MS.gene71949	0.908355	1.87E-81	-1.69E-46
MS.gene056760	MS.gene71949	0.834619	2.70E-56	-1.69E-46
MS.gene056986	MS.gene71949	0.825411	4.76E-54	-1.69E-46
MS.gene057057	MS.gene71949	0.812842	3.46E-51	-1.69E-46
MS.gene057060	MS.gene71949	0.830718	2.51E-55	-1.69E-46
MS.gene057079	MS.gene71949	0.810074	1.38E-50	-1.69E-46
MS.gene057094	MS.gene71949	0.81289	3.38E-51	-1.69E-46
MS.gene057140	MS.gene71949	0.862818	3.87E-64	-1.69E-46
MS.gene057234	MS.gene71949	0.825783	3.88E-54	-1.69E-46
MS.gene057444	MS.gene71949	0.803669	3.12E-49	-1.69E-46
MS.gene057555	MS.gene71949	0.804401	2.20E-49	-1.69E-46
MS.gene057693	MS.gene71949	0.842022	3.33E-58	-1.69E-46
MS.gene057694	MS.gene71949	0.817021	4.09E-52	-1.69E-46
MS.gene057705	MS.gene71949	0.881664	1.99E-70	-1.69E-46
MS.gene05781	MS.gene71949	0.803073	4.15E-49	-1.69E-46
MS.gene058055	MS.gene71949	0.816156	6.39E-52	-1.69E-46
MS.gene058084	MS.gene71949	0.854609	1.10E-61	-1.69E-46
MS.gene058238	MS.gene71949	0.83431	3.23E-56	-1.69E-46
MS.gene058548	MS.gene71949	0.809749	1.62E-50	-1.69E-46
MS.gene058549	MS.gene71949	0.820838	5.55E-53	-1.69E-46
MS.gene058906	MS.gene71949	0.874804	5.07E-68	-1.69E-46
MS.gene058908	MS.gene71949	0.807783	4.27E-50	-1.69E-46
MS.gene058911	MS.gene71949	0.804052	2.60E-49	-1.69E-46
MS.gene059151	MS.gene71949	0.812811	3.51E-51	-1.69E-46
MS.gene059249	MS.gene71949	0.815383	9.51E-52	-1.69E-46
MS.gene05953	MS.gene71949	0.810418	1.16E-50	-1.69E-46
MS.gene05954	MS.gene71949	0.850248	1.92E-60	-1.69E-46
MS.gene059569	MS.gene71949	0.822407	2.41E-53	-1.69E-46
MS.gene05979	MS.gene71949	0.832651	8.37E-56	-1.69E-46
MS.gene059830	MS.gene71949	0.828941	6.80E-55	-1.69E-46
MS.gene059842	MS.gene71949	0.819634	1.05E-52	-1.69E-46
MS.gene059898	MS.gene71949	0.822743	2.01E-53	-1.69E-46
MS.gene059935	MS.gene71949	0.802325	5.92E-49	-1.69E-46
MS.gene06001	MS.gene71949	0.899515	1.84E-77	-1.69E-46
MS.gene06011	MS.gene71949	0.815757	7.85E-52	-1.69E-46
MS.gene060365	MS.gene71949	0.826893	2.11E-54	-1.69E-46
MS.gene060429	MS.gene71949	0.940325	2.83E-100	-1.69E-46
MS.gene060572	MS.gene71949	0.812968	3.25E-51	-1.69E-46
MS.gene060586	MS.gene71949	0.837021	6.65E-57	-1.69E-46
MS.gene060587	MS.gene71949	0.812253	4.65E-51	-1.69E-46
MS.gene060803	MS.gene71949	0.807807	4.22E-50	-1.69E-46
MS.gene060830	MS.gene71949	0.811544	6.64E-51	-1.69E-46
MS.gene060907	MS.gene71949	0.830626	2.64E-55	-1.69E-46
MS.gene060917	MS.gene71949	0.874183	8.24E-68	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene71949	MS.gene063067	PPI
MS.gene71949	MS.gene014181	PPI
MS.gene68113	MS.gene71949	PPI
MS.gene21175	MS.gene71949	PPI
MS.gene71949	MS.gene21175	PPI
MS.gene71949	MS.gene68716	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene71949.t1	MTR_5g012610	100.000	62	0	0	1	62	1	62	2.81e-39	123
MS.gene71949.t1	MTR_4g100900	100.000	62	0	0	1	62	1	62	2.81e-39	123

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene71949.t1	AT5G56670	95.161	62	3	1	62	1	62	6.25e-30	100
MS.gene71949.t1	AT4G29390	95.161	62	3	1	62	1	62	6.25e-30	100
MS.gene71949.t1	AT2G19750	95.161	62	3	1	62	1	62	6.25e-30	100

Find 12 sgRNAs with CRISPR-Local

Find 24 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTGTCTTGCTTTGCAACTTT+TGG	0.146380	8.3:+20187655	None:intergenic
ATTATTGCAGTTGTGGGATT+TGG	0.276718	8.3:-20187447	MS.gene71949:intron
TTGCTCGTGCAGGAAAAGTT+AGG	0.368060	8.3:-20187687	MS.gene71949:CDS
TGCTCGTGCAGGAAAAGTTA+GGG	0.470658	8.3:-20187686	MS.gene71949:CDS
CATGGTTCCCTTGCTCGTGC+AGG	0.506156	8.3:-20187697	MS.gene71949:CDS
AGTTACCGGCGGTGACGAAA+CGG	0.526875	8.3:+20187584	None:intergenic
TGAAATGCAGGTAAAGTGCA+TGG	0.575483	8.3:-20187715	MS.gene71949:intron
GTGGGATTTGGAAAGAAGCG+TGG	0.615049	8.3:-20187435	MS.gene71949:CDS
AATCGCCGTTTCGTCACCGC+CGG	0.622871	8.3:-20187589	MS.gene71949:intron
CGCTTGTGAGCACGTCCTCG+TGG	0.625412	8.3:+20187622	None:intergenic
GCTCGTGCAGGAAAAGTTAG+GGG	0.631196	8.3:-20187685	MS.gene71949:CDS
GACAAGAAGAAGCAGCCACG+AGG	0.688180	8.3:-20187637	MS.gene71949:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TTTTTTTGTTGTTCTGATTT+TGG	-	chr8.3:20187743-20187762	MS.gene71949:intron	20.0%
!	AGAAAGAATGATAAAGTTAC+CGG	+	chr8.3:20187658-20187677	None:intergenic	25.0%
!	ATGATTATTATTGCAGTTGT+GGG	-	chr8.3:20187772-20187791	MS.gene71949:intron	25.0%
!	TATGATTATTATTGCAGTTG+TGG	-	chr8.3:20187771-20187790	MS.gene71949:intron	25.0%
!	TGATTCGATACCTATAAAAT+TGG	-	chr8.3:20187437-20187456	MS.gene71949:CDS	25.0%
!!	GAAATCGAAACCAATTTTAT+AGG	+	chr8.3:20187450-20187469	None:intergenic	25.0%
	AAGAATGATAAAGTTACCGG+CGG	+	chr8.3:20187655-20187674	None:intergenic	35.0%
	ATTATTGCAGTTGTGGGATT+TGG	-	chr8.3:20187778-20187797	MS.gene71949:intron	35.0%
!	TGAATCGACATTTGAAATGC+AGG	-	chr8.3:20187498-20187517	MS.gene71949:intron	35.0%
!	TGTCTTGCTTTGCAACTTTT+GGG	+	chr8.3:20187572-20187591	None:intergenic	35.0%
!	TTGTCTTGCTTTGCAACTTT+TGG	+	chr8.3:20187573-20187592	None:intergenic	35.0%
	TGAAATGCAGGTAAAGTGCA+TGG	-	chr8.3:20187510-20187529	MS.gene71949:intron	40.0%
!	GTCTTGCTTTGCAACTTTTG+GGG	+	chr8.3:20187571-20187590	None:intergenic	40.0%
	TGCTCGTGCAGGAAAAGTTA+GGG	-	chr8.3:20187539-20187558	MS.gene71949:intron	45.0%
	TTGCTCGTGCAGGAAAAGTT+AGG	-	chr8.3:20187538-20187557	MS.gene71949:intron	45.0%
!	AACTTTTCCTGCACGAGCAA+GGG	+	chr8.3:20187538-20187557	None:intergenic	45.0%
!	TAACTTTTCCTGCACGAGCA+AGG	+	chr8.3:20187539-20187558	None:intergenic	45.0%
	GCTCGTGCAGGAAAAGTTAG+GGG	-	chr8.3:20187540-20187559	MS.gene71949:intron	50.0%
	GTGGGATTTGGAAAGAAGCG+TGG	-	chr8.3:20187790-20187809	MS.gene71949:intron	50.0%
	AGTTACCGGCGGTGACGAAA+CGG	+	chr8.3:20187644-20187663	None:intergenic	55.0%
	GACAAGAAGAAGCAGCCACG+AGG	-	chr8.3:20187588-20187607	MS.gene71949:intron	55.0%
	AATCGCCGTTTCGTCACCGC+CGG	-	chr8.3:20187636-20187655	MS.gene71949:CDS	60.0%
	CATGGTTCCCTTGCTCGTGC+AGG	-	chr8.3:20187528-20187547	MS.gene71949:intron	60.0%
	CGCTTGTGAGCACGTCCTCG+TGG	+	chr8.3:20187606-20187625	None:intergenic	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr8.3	gene	20187413	20187834	20187413	ID=MS.gene71949
chr8.3	mRNA	20187413	20187834	20187413	ID=MS.gene71949.t1;Parent=MS.gene71949
chr8.3	exon	20187831	20187834	20187831	ID=MS.gene71949.t1.exon1;Parent=MS.gene71949.t1
chr8.3	CDS	20187831	20187834	20187831	ID=cds.MS.gene71949.t1;Parent=MS.gene71949.t1
chr8.3	exon	20187590	20187727	20187590	ID=MS.gene71949.t1.exon2;Parent=MS.gene71949.t1
chr8.3	CDS	20187590	20187727	20187590	ID=cds.MS.gene71949.t1;Parent=MS.gene71949.t1
chr8.3	exon	20187413	20187459	20187413	ID=MS.gene71949.t1.exon3;Parent=MS.gene71949.t1
chr8.3	CDS	20187413	20187459	20187413	ID=cds.MS.gene71949.t1;Parent=MS.gene71949.t1

Gene Sequence

>MS.gene71949

ATGGGTGAGTTGATTATTATTATTTGATTCGATACCTATAAAATTGGTTTCGATTTCAGTGTTTGTGAAAAGTGAATGAACGCGATGAATCGACATTTGAAATGCAGGTAAAGTGCATGGTTCCCTTGCTCGTGCAGGAAAAGTTAGGGGACAAACCCCAAAAGTTGCAAAGCAAGACAAGAAGAAGCAGCCACGAGGACGTGCTCACAAGCGTATGCAATACAATCGCCGTTTCGTCACCGCCGGTAACTTTATCATTCTTTCTTTTACTTTTCGCTTTACGATTTGTTTTGTTAGATCTGTAGAGCTTGTAAATTTCACAACGTTATTTTTTTTTGTTGTTCTGATTTTGGTTTTGTATGATTATTATTGCAGTTGTGGGATTTGGAAAGAAGCGTGGACCTAACTCATCTGAAAAGTGA

Protein sequence

>MS.gene71949.t1

MGKVHGSLARAGKVRGQTPKVAKQDKKKQPRGRAHKRMQYNRRFVTAVVGFGKKRGPNSSEK