AlfalfaGEDB | MS.gene21425

M. sativa cultivar XinJiangDaYe / MS.gene21425

KEGG: K11252
GO: GO:0000786 GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005515 GO:0005575 GO:0032991 GO:0032993 GO:0044815 GO:0046982 GO:0046983 GO:0097159 GO:1901363
PFAM: PF00125.27

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene21425.t1	XP_013469298.1	100	137	0	0	1	137	1	137	8.20E-42	179.9

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene21425.t1	Q1S9I9	100.0	91	0	0	47	137	58	148	3.7e-43	175.6

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene21425.t1	I3S6Z4	100.0	137	0	0	1	137	1	137	5.9e-42	179.9

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049705	MS.gene21425	0.806878	6.64E-50	-1.69E-46
MS.gene049989	MS.gene21425	0.809905	1.50E-50	-1.69E-46
MS.gene050648	MS.gene21425	0.810135	1.34E-50	-1.69E-46
MS.gene051263	MS.gene21425	0.871096	8.86E-67	-1.69E-46
MS.gene051478	MS.gene21425	0.809608	1.74E-50	-1.69E-46
MS.gene051629	MS.gene21425	0.813309	2.73E-51	-1.69E-46
MS.gene055957	MS.gene21425	0.840873	6.69E-58	-1.69E-46
MS.gene056041	MS.gene21425	0.80602	1.01E-49	-1.69E-46
MS.gene056042	MS.gene21425	0.839103	1.94E-57	-1.69E-46
MS.gene058197	MS.gene21425	0.802083	6.64E-49	-1.69E-46
MS.gene058198	MS.gene21425	0.80772	4.40E-50	-1.69E-46
MS.gene059300	MS.gene21425	0.815206	1.04E-51	-1.69E-46
MS.gene06057	MS.gene21425	0.809828	1.56E-50	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene21425.t1	MTR_1g492910	100.000	137	0	0	1	137	1	137	2.30e-95	271
MS.gene21425.t1	MTR_1g090780	99.270	137	1	0	1	137	1	137	1.25e-94	269
MS.gene21425.t1	MTR_4g063240	86.395	147	10	3	1	137	1	147	7.09e-81	234
MS.gene21425.t1	MTR_4g063220	87.162	148	8	3	1	137	1	148	3.56e-77	225
MS.gene21425.t1	MTR_4g063990	87.162	148	8	3	1	137	1	148	3.56e-77	225
MS.gene21425.t1	MTR_4g063200	87.162	148	8	3	1	137	1	148	3.56e-77	225
MS.gene21425.t1	MTR_4g064020	87.162	148	8	3	1	137	1	148	3.56e-77	225
MS.gene21425.t1	MTR_4g071180	87.162	148	8	3	1	137	1	148	3.12e-76	223
MS.gene21425.t1	MTR_4g070240	87.162	148	8	3	1	137	1	148	3.12e-76	223
MS.gene21425.t1	MTR_4g088025	89.510	143	9	3	1	137	1	143	6.36e-76	222
MS.gene21425.t1	MTR_4g070245	85.811	148	10	3	1	137	1	148	1.45e-74	219
MS.gene21425.t1	MTR_4g063275	84.459	148	12	3	1	137	1	148	6.43e-74	217
MS.gene21425.t1	MTR_4g064983	86.111	144	9	3	1	133	1	144	1.53e-72	217
MS.gene21425.t1	MTR_7g099960	95.413	109	4	1	29	137	31	138	2.52e-64	192
MS.gene21425.t1	MTR_1g068600	93.578	109	5	1	29	137	30	136	2.97e-63	189
MS.gene21425.t1	MTR_2g084480	61.798	89	34	0	48	136	136	224	1.85e-37	127
MS.gene21425.t1	MTR_8g069990	69.663	89	24	1	8	96	3	88	7.70e-37	121
MS.gene21425.t1	MTR_4g063270	90.196	51	5	0	75	125	1	51	5.24e-27	95.5
MS.gene21425.t1	MTR_7g114580	90.244	41	4	0	97	137	11	51	5.86e-20	77.8
MS.gene21425.t1	MTR_4g032777	36.364	99	58	1	28	121	12	110	3.88e-13	62.4
MS.gene21425.t1	MTR_2g083800	67.273	55	15	2	8	61	11	63	1.75e-11	56.6

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene21425.t1	AT2G37470	88.406	138	14	2	1	136	1	138	4.90e-75	219
MS.gene21425.t1	AT3G45980	83.333	150	12	3	1	137	1	150	2.60e-74	218
MS.gene21425.t1	AT2G28720	82.119	151	13	2	1	137	1	151	4.16e-74	218
MS.gene21425.t1	AT3G53650	86.331	139	16	3	1	137	1	138	1.55e-71	211
MS.gene21425.t1	AT1G07790	83.333	150	10	4	1	137	1	148	1.89e-71	211
MS.gene21425.t1	AT5G59910	87.313	134	15	2	6	137	17	150	6.51e-70	207
MS.gene21425.t1	AT3G46030	89.147	129	12	2	11	137	17	145	2.25e-69	206
MS.gene21425.t1	AT5G22880	80.282	142	14	2	10	137	4	145	1.94e-66	198
MS.gene21425.t1	AT5G02570	84.173	139	13	2	1	137	1	132	2.96e-66	197
MS.gene21425.t1	AT3G09480	79.562	137	17	2	1	137	1	126	2.00e-62	187
MS.gene21425.t1	AT1G08170	56.989	93	40	0	42	134	143	235	6.80e-38	129

Find 35 sgRNAs with CRISPR-Local

Find 33 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TATTCAAGAGCTAGTGAATT+TGG	0.197215	1.4:-72062597	None:intergenic
GCCGTTCGTCTTGTTCTTCC+TGG	0.234822	1.4:+72062530	MS.gene21425:CDS
CTTGAAGATGTAGATCTTGT+AGG	0.279657	1.4:-72062348	None:intergenic
TCAGTTGAGGATGCTTCCTT+TGG	0.300529	1.4:-72062290	None:intergenic
GCGGGTTTCTTCTCTCCCTT+TGG	0.352007	1.4:-72062209	None:intergenic
TCAGCTGGTGCCTTCTCTGC+AGG	0.357925	1.4:-72062242	None:intergenic
CGGTATCTCAAGCAAGGCTA+TGG	0.396652	1.4:+72062397	MS.gene21425:CDS
CAGTTGAGGATGCTTCCTTT+GGG	0.416445	1.4:-72062289	None:intergenic
AGTGAATTTGGTGACAGCTT+TGG	0.420989	1.4:-72062585	None:intergenic
CTACAAGATCTACATCTTCA+AGG	0.431521	1.4:+72062349	MS.gene21425:CDS
TCCCTCGGAGACAGCGTGCT+TGG	0.447024	1.4:-72062561	None:intergenic
CTTCTTCTTCTTATCAGTTG+AGG	0.448679	1.4:-72062303	None:intergenic
CATCTTCAAGGTTCTCAAGC+AGG	0.470454	1.4:+72062361	MS.gene21425:CDS
TCTGCAGGCTTCTTCTCTGC+GGG	0.470993	1.4:-72062227	None:intergenic
GCGTGCTTGGCGAGTTCACC+AGG	0.485011	1.4:-72062548	None:intergenic
AGAGAAGAAGCCTGCAGAGA+AGG	0.499836	1.4:+72062232	MS.gene21425:CDS
CTCTGCAGGCTTCTTCTCTG+CGG	0.503353	1.4:-72062228	None:intergenic
GGTGACAGCTTTGGTTCCCT+CGG	0.503972	1.4:-72062576	None:intergenic
CTGAATCTCCCGAGAGGTGA+TGG	0.509224	1.4:-72062504	None:intergenic
TCAATCAACAATGGCACCAA+AGG	0.515027	1.4:+72062193	None:intergenic
GGTATCTCAAGCAAGGCTAT+GGG	0.525343	1.4:+72062398	MS.gene21425:CDS
TTGCTTGAGATACCGATGTC+AGG	0.547522	1.4:-72062389	None:intergenic
GAAACCTACCATCACCTCTC+GGG	0.549293	1.4:+72062496	MS.gene21425:CDS
AGAAACCTACCATCACCTCT+CGG	0.550335	1.4:+72062495	MS.gene21425:CDS
CAATCAACAATGGCACCAAA+GGG	0.556792	1.4:+72062194	None:intergenic
TATCTTTGAGAAGCTCGCTC+AGG	0.560608	1.4:+72062445	MS.gene21425:CDS
GCCAAGCACGCTGTCTCCGA+GGG	0.566568	1.4:+72062560	MS.gene21425:CDS
CGCCAAGCACGCTGTCTCCG+AGG	0.573257	1.4:+72062559	MS.gene21425:CDS
TGACATCGGTATCTCAAGCA+AGG	0.623015	1.4:+72062391	MS.gene21425:CDS
ACCAGGAAGAACAAGACGAA+CGG	0.639588	1.4:-72062531	None:intergenic
AAGCAGGTTCATCCTGACAT+CGG	0.661631	1.4:+72062377	MS.gene21425:CDS
AGCAGAGAAGAAGATCCCAA+AGG	0.665852	1.4:+72062274	MS.gene21425:CDS
GTATCTCAAGCAAGGCTATG+GGG	0.674185	1.4:+72062399	MS.gene21425:CDS
ATCTCCCGAGAGGTGATGGT+AGG	0.682571	1.4:-72062500	None:intergenic
GGCAGTCTGAATCTCCCGAG+AGG	0.728312	1.4:-72062510	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	CTACAAGATCTACATCTTCA+AGG	+	chr1.4:72062349-72062368	MS.gene21425:CDS	35.0%
	CTTCTTCTTCTTATCAGTTG+AGG	-	chr1.4:72062306-72062325	None:intergenic	35.0%
	CTTGAAGATGTAGATCTTGT+AGG	-	chr1.4:72062351-72062370	None:intergenic	35.0%
!	AGTGAATTTGGTGACAGCTT+TGG	-	chr1.4:72062588-72062607	None:intergenic	40.0%
	AAGCAGGTTCATCCTGACAT+CGG	+	chr1.4:72062377-72062396	MS.gene21425:CDS	45.0%
	ACCAGGAAGAACAAGACGAA+CGG	-	chr1.4:72062534-72062553	None:intergenic	45.0%
	AGAAACCTACCATCACCTCT+CGG	+	chr1.4:72062495-72062514	MS.gene21425:CDS	45.0%
	AGCAGAGAAGAAGATCCCAA+AGG	+	chr1.4:72062274-72062293	MS.gene21425:CDS	45.0%
	CAGTTGAGGATGCTTCCTTT+GGG	-	chr1.4:72062292-72062311	None:intergenic	45.0%
	CATCTTCAAGGTTCTCAAGC+AGG	+	chr1.4:72062361-72062380	MS.gene21425:CDS	45.0%
	GGTATCTCAAGCAAGGCTAT+GGG	+	chr1.4:72062398-72062417	MS.gene21425:CDS	45.0%
	GTATCTCAAGCAAGGCTATG+GGG	+	chr1.4:72062399-72062418	MS.gene21425:CDS	45.0%
	TCAGTTGAGGATGCTTCCTT+TGG	-	chr1.4:72062293-72062312	None:intergenic	45.0%
	TGACATCGGTATCTCAAGCA+AGG	+	chr1.4:72062391-72062410	MS.gene21425:CDS	45.0%
	TTGCTTGAGATACCGATGTC+AGG	-	chr1.4:72062392-72062411	None:intergenic	45.0%
!	TATCTTTGAGAAGCTCGCTC+AGG	+	chr1.4:72062445-72062464	MS.gene21425:CDS	45.0%
!	TCTGCTTTCGTTTTCTCAGC+TGG	-	chr1.4:72062260-72062279	None:intergenic	45.0%
	AGAGAAGAAGCCTGCAGAGA+AGG	+	chr1.4:72062232-72062251	MS.gene21425:CDS	50.0%
	CGGTATCTCAAGCAAGGCTA+TGG	+	chr1.4:72062397-72062416	MS.gene21425:CDS	50.0%
	GAAACCTACCATCACCTCTC+GGG	+	chr1.4:72062496-72062515	MS.gene21425:CDS	50.0%
	CTCTGCAGGCTTCTTCTCTG+CGG	-	chr1.4:72062231-72062250	None:intergenic	55.0%
	CTGAATCTCCCGAGAGGTGA+TGG	-	chr1.4:72062507-72062526	None:intergenic	55.0%
	GCGGGTTTCTTCTCTCCCTT+TGG	-	chr1.4:72062212-72062231	None:intergenic	55.0%
	TCTGCAGGCTTCTTCTCTGC+GGG	-	chr1.4:72062230-72062249	None:intergenic	55.0%
!	ATCTCCCGAGAGGTGATGGT+AGG	-	chr1.4:72062503-72062522	None:intergenic	55.0%
!	GCCGTTCGTCTTGTTCTTCC+TGG	+	chr1.4:72062530-72062549	MS.gene21425:CDS	55.0%
!	GGTGACAGCTTTGGTTCCCT+CGG	-	chr1.4:72062579-72062598	None:intergenic	55.0%
	GGCAGTCTGAATCTCCCGAG+AGG	-	chr1.4:72062513-72062532	None:intergenic	60.0%
	TCAGCTGGTGCCTTCTCTGC+AGG	-	chr1.4:72062245-72062264	None:intergenic	60.0%
	GCCAAGCACGCTGTCTCCGA+GGG	+	chr1.4:72062560-72062579	MS.gene21425:CDS	65.0%
	GCGTGCTTGGCGAGTTCACC+AGG	-	chr1.4:72062551-72062570	None:intergenic	65.0%
	TCCCTCGGAGACAGCGTGCT+TGG	-	chr1.4:72062564-72062583	None:intergenic	65.0%
	CGCCAAGCACGCTGTCTCCG+AGG	+	chr1.4:72062559-72062578	MS.gene21425:CDS	70.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.4	gene	72062203	72062616	72062203	ID=MS.gene21425
chr1.4	mRNA	72062203	72062616	72062203	ID=MS.gene21425.t1;Parent=MS.gene21425
chr1.4	exon	72062203	72062616	72062203	ID=MS.gene21425.t1.exon1;Parent=MS.gene21425.t1
chr1.4	CDS	72062203	72062616	72062203	ID=cds.MS.gene21425.t1;Parent=MS.gene21425.t1

Gene Sequence

>MS.gene21425

ATGGCACCAAAGGGAGAGAAGAAACCCGCAGAGAAGAAGCCTGCAGAGAAGGCACCAGCTGAGAAAACGAAAGCAGAGAAGAAGATCCCAAAGGAAGCATCCTCAACTGATAAGAAGAAGAAGAGAAACAAGAAGAGTGTTGAAACCTACAAGATCTACATCTTCAAGGTTCTCAAGCAGGTTCATCCTGACATCGGTATCTCAAGCAAGGCTATGGGGATCATGAACAGTTTCATCAATGATATCTTTGAGAAGCTCGCTCAGGAATCATCTAGACTCGCTCGCTACAACAAGAAACCTACCATCACCTCTCGGGAGATTCAGACTGCCGTTCGTCTTGTTCTTCCTGGTGAACTCGCCAAGCACGCTGTCTCCGAGGGAACCAAAGCTGTCACCAAATTCACTAGCTCTTGA

Protein sequence

>MS.gene21425.t1

MAPKGEKKPAEKKPAEKAPAEKTKAEKKIPKEASSTDKKKKRNKKSVETYKIYIFKVLKQVHPDIGISSKAMGIMNSFINDIFEKLAQESSRLARYNKKPTITSREIQTAVRLVLPGELAKHAVSEGTKAVTKFTSS