AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene21848

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene21848.t1 MTR_5g006290 91.241 274 19 3 1 272 1 271 0.0 513
MS.gene21848.t1 MTR_5g006290 90.945 254 18 3 1 252 1 251 6.94e-171 473
MS.gene21848.t1 MTR_1g104500 71.168 274 73 4 1 272 1 270 5.65e-146 411
MS.gene21848.t1 MTR_5g006300 79.920 249 36 3 12 252 8 250 4.70e-145 408
MS.gene21848.t1 MTR_2g099720 36.149 296 149 6 14 272 53 345 5.14e-61 197
MS.gene21848.t1 MTR_1g104513 56.061 66 26 2 209 272 1 65 2.78e-15 72.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene21848.t1 AT4G14930 58.271 266 101 5 14 272 16 278 7.52e-109 317
MS.gene21848.t1 AT4G14930 58.271 266 101 5 14 272 16 278 1.53e-108 317
MS.gene21848.t1 AT1G72880 47.549 204 99 3 14 216 63 259 2.35e-59 193
MS.gene21848.t1 AT1G72880 47.549 204 99 3 14 216 63 259 2.35e-59 193

Find 63 sgRNAs with CRISPR-Local

Find 229 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTTTCTTCCTTCGCTGTTTC+TGG 0.110041 5.2:-1131058 MS.gene21848:CDS
TCGTACTGAGTGGCATAAAT+AGG 0.244132 5.2:-1130973 MS.gene21848:CDS
CGTACTGAGTGGCATAAATA+GGG 0.336503 5.2:-1130972 MS.gene21848:CDS
CGCTCCTGGCTTAAGAGCTT+TGG 0.339950 5.2:-1131212 MS.gene21848:CDS
ACTGCTGATTTCTCAGTATC+AGG 0.346261 5.2:+1131142 None:intergenic
GCCAAACAAGTTCATATTCA+TGG 0.356932 5.2:-1131085 MS.gene21848:CDS
CCCGCTGATTGCACTTCTCT+TGG 0.364382 5.2:-1131031 MS.gene21848:CDS
CTCGCAAATAAAAGATGTTC+AGG 0.372141 5.2:+1128641 None:intergenic
ACCAGTACTGAGTTTATGAT+TGG 0.387156 5.2:+1130084 None:intergenic
TCCATGAATATGAACTTGTT+TGG 0.390536 5.2:+1131084 None:intergenic
TCATATGAAATGGAGATAGA+AGG 0.392459 5.2:+1130402 None:intergenic
AACATACCAGTCATATGAAA+TGG 0.413011 5.2:+1130392 None:intergenic
TGAACTTGTTTGGCAGCTAT+AGG 0.420107 5.2:+1131094 None:intergenic
TATTCTATGTCCAGGGTTGA+AGG 0.439800 5.2:-1130153 MS.gene21848:intron
TGTAGTAGGAAAGAGTGATT+TGG 0.440018 5.2:+1131003 None:intergenic
GGGTTACAAGCTAACGAGGC+AGG 0.444468 5.2:-1128783 MS.gene21848:CDS
CTATCTCCATTTCATATGAC+TGG 0.452640 5.2:-1130398 MS.gene21848:intron
TACCTTATGGTTAGCAACAT+TGG 0.464242 5.2:+1130005 None:intergenic
GTTTACTCAGGCACTGTAGC+TGG 0.466587 5.2:-1130453 MS.gene21848:CDS
AGTAACAAAATCGATGGAAG+AGG 0.475243 5.2:-1131293 None:intergenic
GGGTAAAAGTATCTTTAAGA+TGG 0.475283 5.2:-1128762 MS.gene21848:CDS
AACCAATGTTGCTAACCATA+AGG 0.509023 5.2:-1130007 MS.gene21848:intron
AGTACGAGATCGGGTGTAGT+AGG 0.510543 5.2:+1130989 None:intergenic
ATGCCACTCAGTACGAGATC+GGG 0.512957 5.2:+1130980 None:intergenic
GACGATGGAATCGACGCTCC+TGG 0.513493 5.2:-1131226 MS.gene21848:CDS
TCCAATCATAAACTCAGTAC+TGG 0.516828 5.2:-1130085 MS.gene21848:CDS
TTATGGTTAGCAACATTGGT+TGG 0.519228 5.2:+1130009 None:intergenic
AAAGTATCTTTAAGATGGGA+TGG 0.524804 5.2:-1128757 MS.gene21848:CDS
TACAGATCACAAATCTTTGA+AGG 0.525967 5.2:-1127833 MS.gene21848:CDS
TTCCCTTTCAGTGTTTACTC+AGG 0.528508 5.2:-1130465 MS.gene21848:intron
TATGCCACTCAGTACGAGAT+CGG 0.530893 5.2:+1130979 None:intergenic
GGTAAAAGTATCTTTAAGAT+GGG 0.542844 5.2:-1128761 MS.gene21848:CDS
CACTGTAGCTGGAGCTCGAG+AGG 0.542909 5.2:-1130442 MS.gene21848:CDS
AGAAGGGATATTGTTGAAGA+AGG 0.544062 5.2:+1130419 None:intergenic
GACACCAAAGCTCTTAAGCC+AGG 0.545144 5.2:+1131208 None:intergenic
TCCAAGAGAAGTGCAATCAG+CGG 0.552211 5.2:+1131030 None:intergenic
CATATGAAATGGAGATAGAA+GGG 0.554398 5.2:+1130403 None:intergenic
CCAAGAGAAGTGCAATCAGC+GGG 0.556534 5.2:+1131031 None:intergenic
AGAAACAGCGAAGGAAGAAA+CGG 0.565756 5.2:+1131060 None:intergenic
GATCACAAATCTTTGAAGGC+GGG 0.566869 5.2:-1127829 MS.gene21848:CDS
AATGCAAACTTGTGCAGCAA+GGG 0.572061 5.2:+1130107 None:intergenic
ATAAAGAAACAAAGCTACCC+TGG 0.585747 5.2:-1130057 MS.gene21848:CDS
AACATTAACAATAAGAGAGG+AGG 0.591884 5.2:-1131268 MS.gene21848:CDS
ATCATCATCATAATCACGTA+GGG 0.594808 5.2:+1127855 None:intergenic
GTAACAAAATCGATGGAAGA+GGG 0.604074 5.2:-1131292 None:intergenic
GGTTACAAGCTAACGAGGCA+GGG 0.604854 5.2:-1128782 MS.gene21848:CDS
GGTAACATTAACAATAAGAG+AGG 0.605178 5.2:-1131271 MS.gene21848:CDS
TATCATCATCATAATCACGT+AGG 0.609428 5.2:+1127854 None:intergenic
CAGTTGGTCACAGCATCACA+TGG 0.611704 5.2:-1131123 MS.gene21848:CDS
GAATGCAAACTTGTGCAGCA+AGG 0.611800 5.2:+1130106 None:intergenic
TGAAGGAAAGAGTAATCCAC+AGG 0.618509 5.2:-1130136 MS.gene21848:CDS
GGGAGTACCAGAAACAGCGA+AGG 0.623127 5.2:+1131051 None:intergenic
GATACTGAGAAATCAGCAGT+TGG 0.641119 5.2:-1131139 MS.gene21848:CDS
CAAGTCACCTCTGAGACAGA+AGG 0.641811 5.2:-1128731 MS.gene21848:CDS
TCTTCGTCCTTCTGTCTCAG+AGG 0.641863 5.2:+1128724 None:intergenic
GCAGCAAGGGCAAAGTCCTG+TGG 0.646391 5.2:+1130120 None:intergenic
GTATCTTTAAGATGGGATGG+AGG 0.656769 5.2:-1128754 MS.gene21848:CDS
ATAAATAGGGGTAGCAACTG+CGG 0.656882 5.2:-1130959 MS.gene21848:CDS
AGATCACAAATCTTTGAAGG+CGG 0.659879 5.2:-1127830 MS.gene21848:CDS
ATTCTGATAACAAACGACGA+TGG 0.666629 5.2:-1131241 MS.gene21848:CDS
GTACTGAGTGGCATAAATAG+GGG 0.669770 5.2:-1130971 MS.gene21848:CDS
AAAAGGGTTACAAGCTAACG+AGG 0.689847 5.2:-1128787 MS.gene21848:intron
ACACCCGATCTCGTACTGAG+TGG 0.722593 5.2:-1130983 MS.gene21848:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATAAGTAAAAACAATTTA+CGG + chr5.2:1128312-1128331 None:intergenic 10.0%
!!! AAATTTTTTATTGTTACTTT+AGG - chr5.2:1129405-1129424 MS.gene21848:intron 10.0%
!! AAAATAGTATAAATCATAAG+AGG + chr5.2:1128547-1128566 None:intergenic 15.0%
!! AAATAGTATAAATCATAAGA+GGG + chr5.2:1128546-1128565 None:intergenic 15.0%
!! ATATTAAATGAAAATACAGA+AGG + chr5.2:1130175-1130194 None:intergenic 15.0%
!!! AGAATATAATCAATTTTCTT+AGG + chr5.2:1129498-1129517 None:intergenic 15.0%
!!! TAAATAGAACAATTTTTAAC+AGG + chr5.2:1128447-1128466 None:intergenic 15.0%
!!! TACTATTTTTATTTTTGCTA+AGG - chr5.2:1128558-1128577 MS.gene21848:intron 15.0%
!! AAAGTTCAACTTAACAAATA+TGG + chr5.2:1128778-1128797 None:intergenic 20.0%
!! AAATGTAAACTTCAATATGT+AGG + chr5.2:1130198-1130217 None:intergenic 20.0%
!! AACTACATTATTATTCATTG+CGG + chr5.2:1131194-1131213 None:intergenic 20.0%
!! AAGATTTATATGACAATTTC+TGG + chr5.2:1129546-1129565 None:intergenic 20.0%
!! AAGTTTACATTTGTATTCAA+AGG - chr5.2:1130206-1130225 MS.gene21848:intron 20.0%
!! AATAAGCATAATACATGATA+AGG - chr5.2:1129326-1129345 MS.gene21848:intron 20.0%
!! ACAATTATAAGCTGATTAAT+TGG + chr5.2:1128806-1128825 None:intergenic 20.0%
!! ACTTATTACAATATACCTTA+TGG + chr5.2:1129116-1129135 None:intergenic 20.0%
!! ATAGTATTTATATCAGATCT+GGG + chr5.2:1130693-1130712 None:intergenic 20.0%
!! ATCTTCTGTAACTAATTTAT+TGG - chr5.2:1130276-1130295 MS.gene21848:intron 20.0%
!! CTATTTATTTCAAATGACAA+AGG - chr5.2:1128460-1128479 MS.gene21848:intron 20.0%
!! GATTTATTCATAATATATGC+TGG - chr5.2:1129350-1129369 MS.gene21848:intron 20.0%
!! TATAGTATTTATATCAGATC+TGG + chr5.2:1130694-1130713 None:intergenic 20.0%
!! TATTTGAATTTGACAAAAAG+TGG - chr5.2:1130824-1130843 MS.gene21848:intron 20.0%
!!! AGCAATGTAAATTATTTCTT+TGG + chr5.2:1128364-1128383 None:intergenic 20.0%
!!! GCAATGTAAATTATTTCTTT+GGG + chr5.2:1128363-1128382 None:intergenic 20.0%
!!! TTTTTTCTTCAAACTTTTGT+AGG - chr5.2:1131218-1131237 MS.gene21848:CDS 20.0%
! AAAGTCTAAAGGTGATAAAA+TGG + chr5.2:1128191-1128210 None:intergenic 25.0%
! AACATAAATTAATGCTGTGA+TGG - chr5.2:1129303-1129322 MS.gene21848:intron 25.0%
! AACTAAAAAACTTACTTGGA+TGG - chr5.2:1131138-1131157 MS.gene21848:CDS 25.0%
! AATTTGACAGGCATTATATT+AGG - chr5.2:1129429-1129448 MS.gene21848:intron 25.0%
! ACACTAACAAACAATATAAG+TGG + chr5.2:1128259-1128278 None:intergenic 25.0%
! AGCATATTGTCTAATACTTT+CGG - chr5.2:1130549-1130568 MS.gene21848:intron 25.0%
! ATATTCAAAAAACATCTTCC+AGG + chr5.2:1129069-1129088 None:intergenic 25.0%
! ATTATGCAACATTTCAATGA+AGG + chr5.2:1130061-1130080 None:intergenic 25.0%
! GAAGAACTAAAAAACTTACT+TGG - chr5.2:1131134-1131153 MS.gene21848:CDS 25.0%
! GTATATATTGTCATGATATC+AGG + chr5.2:1129954-1129973 None:intergenic 25.0%
! TACTCAAATAAACAAACACA+CGG + chr5.2:1129836-1129855 None:intergenic 25.0%
! TAGTATTTATATCAGATCTG+GGG + chr5.2:1130692-1130711 None:intergenic 25.0%
! TATTCAAAAAACATCTTCCA+GGG + chr5.2:1129068-1129087 None:intergenic 25.0%
! TCATTGAAATGTTGCATAAT+GGG - chr5.2:1130061-1130080 MS.gene21848:CDS 25.0%
! TGAAAAGCTATTGCTATAAA+AGG + chr5.2:1128907-1128926 None:intergenic 25.0%
! TTATGCAACATTTCAATGAA+GGG + chr5.2:1130060-1130079 None:intergenic 25.0%
! TTCATTGAAATGTTGCATAA+TGG - chr5.2:1130060-1130079 MS.gene21848:CDS 25.0%
! TTGTGTGTTATGATAATTTG+TGG - chr5.2:1129711-1129730 MS.gene21848:intron 25.0%
! TTGTTTATTTGAGTATGTGT+TGG - chr5.2:1129841-1129860 MS.gene21848:intron 25.0%
!! ATAGCAAATTTTTATCCTTG+TGG - chr5.2:1129875-1129894 MS.gene21848:intron 25.0%
!! TGTGTGTTATGATAATTTGT+GGG - chr5.2:1129712-1129731 MS.gene21848:intron 25.0%
!!! GGTAAAAGTATCTTTAAGAT+GGG - chr5.2:1130344-1130363 MS.gene21848:intron 25.0%
!!! TTTAGTTCTTCCATATAACT+TGG + chr5.2:1131125-1131144 None:intergenic 25.0%
AACATACCAGTCATATGAAA+TGG + chr5.2:1128716-1128735 None:intergenic 30.0%
AACATTAACAATAAGAGAGG+AGG - chr5.2:1127837-1127856 MS.gene21848:CDS 30.0%
AACTTACGCACAATAAAACT+TGG + chr5.2:1129523-1129542 None:intergenic 30.0%
AATGAATGTCTCGAGTATTA+TGG - chr5.2:1128838-1128857 MS.gene21848:intron 30.0%
ACAGATACACCTAAAAACTT+TGG - chr5.2:1130425-1130444 MS.gene21848:CDS 30.0%
ACTAATTACACAAACAGTGA+CGG + chr5.2:1128618-1128637 None:intergenic 30.0%
ACTCAAATAAACAAACACAC+GGG + chr5.2:1129835-1129854 None:intergenic 30.0%
ACTCTGTTTATAATAAGCAG+TGG + chr5.2:1131054-1131073 None:intergenic 30.0%
AGTATTTATATCAGATCTGG+GGG + chr5.2:1130691-1130710 None:intergenic 30.0%
ATCATCATCATAATCACGTA+GGG + chr5.2:1131253-1131272 None:intergenic 30.0%
CATATGAAATGGAGATAGAA+GGG + chr5.2:1128705-1128724 None:intergenic 30.0%
CCAAAACAATAAATTCCACA+AGG + chr5.2:1129893-1129912 None:intergenic 30.0%
CTAGTTAACTCCTTATGAAA+GGG + chr5.2:1129637-1129656 None:intergenic 30.0%
CTTTCCACTAATTTACAATG+CGG + chr5.2:1129979-1129998 None:intergenic 30.0%
GAAATAGATGCCAAGTTATA+TGG - chr5.2:1131112-1131131 MS.gene21848:CDS 30.0%
GCATTGTAAATTAGTGGAAA+GGG - chr5.2:1129978-1129997 MS.gene21848:intron 30.0%
GGTAACATTAACAATAAGAG+AGG - chr5.2:1127834-1127853 MS.gene21848:CDS 30.0%
TACAGATCACAAATCTTTGA+AGG - chr5.2:1131272-1131291 MS.gene21848:CDS 30.0%
TAGTCAAGCACAATATTAAG+CGG + chr5.2:1130606-1130625 None:intergenic 30.0%
TAGTTAACTCCTTATGAAAG+GGG + chr5.2:1129636-1129655 None:intergenic 30.0%
TATCATCATCATAATCACGT+AGG + chr5.2:1131254-1131273 None:intergenic 30.0%
TATCATGTTTGACCATTTGA+AGG - chr5.2:1128928-1128947 MS.gene21848:intron 30.0%
TATGCAACATTTCAATGAAG+GGG + chr5.2:1130059-1130078 None:intergenic 30.0%
TCATATGAAATGGAGATAGA+AGG + chr5.2:1128706-1128725 None:intergenic 30.0%
TCTAGTTAACTCCTTATGAA+AGG + chr5.2:1129638-1129657 None:intergenic 30.0%
TTATTATTCATTGCGGAAAG+AGG + chr5.2:1131187-1131206 None:intergenic 30.0%
! AACAGCATCTTTTTGTAACA+AGG + chr5.2:1130091-1130110 None:intergenic 30.0%
! ACAGCATCTTTTTGTAACAA+GGG + chr5.2:1130090-1130109 None:intergenic 30.0%
! AGGACTATAGCTCTATTAAT+AGG - chr5.2:1129449-1129468 MS.gene21848:intron 30.0%
! CCTTGTGGAATTTATTGTTT+TGG - chr5.2:1129890-1129909 MS.gene21848:intron 30.0%
! GATGATGTACCAAAGTTTTT+AGG + chr5.2:1130437-1130456 None:intergenic 30.0%
! GTTACTTTAGGTAATTTGAC+AGG - chr5.2:1129417-1129436 MS.gene21848:intron 30.0%
! TCCATGAATATGAACTTGTT+TGG + chr5.2:1128024-1128043 None:intergenic 30.0%
! TTCAAACTTTTGTAGGTAAG+AGG - chr5.2:1131225-1131244 MS.gene21848:CDS 30.0%
!! AAAATTTGCTATTCAACCAG+TGG + chr5.2:1129867-1129886 None:intergenic 30.0%
!! AAAGTATCTTTAAGATGGGA+TGG - chr5.2:1130348-1130367 MS.gene21848:intron 30.0%
!! AATGAACACAAGTTGATATG+TGG + chr5.2:1130910-1130929 None:intergenic 30.0%
!! GGGTAAAAGTATCTTTAAGA+TGG - chr5.2:1130343-1130362 MS.gene21848:intron 30.0%
!! GTGTTGTTGTTTATATTGCT+TGG + chr5.2:1130888-1130907 None:intergenic 30.0%
!! TGAAGTTGTTTTGCACTATT+AGG - chr5.2:1130759-1130778 MS.gene21848:intron 30.0%
!!! GTTTTTAGGTGTATCTGTAT+TGG + chr5.2:1130423-1130442 None:intergenic 30.0%
AACCAATGTTGCTAACCATA+AGG - chr5.2:1129098-1129117 MS.gene21848:intron 35.0%
ACTACCGCATTGTAAATTAG+TGG - chr5.2:1129972-1129991 MS.gene21848:intron 35.0%
AGAAGGGATATTGTTGAAGA+AGG + chr5.2:1128689-1128708 None:intergenic 35.0%
ATAAAGAAACAAAGCTACCC+TGG - chr5.2:1129048-1129067 MS.gene21848:intron 35.0%
ATCTTGACAAAATATGTGGG+GGG + chr5.2:1131028-1131047 None:intergenic 35.0%
ATGAAGAAAAGAGAGACAGA+AGG + chr5.2:1130799-1130818 None:intergenic 35.0%
ATGCAACATTTCAATGAAGG+GGG + chr5.2:1130058-1130077 None:intergenic 35.0%
ATTCTGATAACAAACGACGA+TGG - chr5.2:1127864-1127883 MS.gene21848:CDS 35.0%
ATTGGACTTATTATCAGTGG+CGG - chr5.2:1130633-1130652 MS.gene21848:intron 35.0%
CGCATTGTAAATTAGTGGAA+AGG - chr5.2:1129977-1129996 MS.gene21848:intron 35.0%
CGGATCTTGACAAAATATGT+GGG + chr5.2:1131031-1131050 None:intergenic 35.0%
CGGTAGTTATATCCAAAGAA+TGG - chr5.2:1129605-1129624 MS.gene21848:intron 35.0%
CTATCTCCATTTCATATGAC+TGG - chr5.2:1128707-1128726 MS.gene21848:CDS 35.0%
CTCAAATAAACAAACACACG+GGG + chr5.2:1129834-1129853 None:intergenic 35.0%
CTCGCAAATAAAAGATGTTC+AGG + chr5.2:1130467-1130486 None:intergenic 35.0%
CTGTTTATAATAAGCAGTGG+CGG + chr5.2:1131051-1131070 None:intergenic 35.0%
GAATTTGACAAAAAGTGGCT+AGG - chr5.2:1130829-1130848 MS.gene21848:intron 35.0%
GACATAGAATAGCCTTCAAA+TGG + chr5.2:1128943-1128962 None:intergenic 35.0%
GATCTTGACAAAATATGTGG+GGG + chr5.2:1131029-1131048 None:intergenic 35.0%
GATTCGAACCATTGATCTTA+AGG - chr5.2:1130856-1130875 MS.gene21848:intron 35.0%
GCCAAACAAGTTCATATTCA+TGG - chr5.2:1128020-1128039 MS.gene21848:intron 35.0%
GGATCTTGACAAAATATGTG+GGG + chr5.2:1131030-1131049 None:intergenic 35.0%
GTATTTATATCAGATCTGGG+GGG + chr5.2:1130690-1130709 None:intergenic 35.0%
GTCATTGGACTTATTATCAG+TGG - chr5.2:1130630-1130649 MS.gene21848:intron 35.0%
GTCTAAAGGTGATAAAATGG+TGG + chr5.2:1128188-1128207 None:intergenic 35.0%
TAACTACCGTGATTTCATAG+AGG + chr5.2:1129594-1129613 None:intergenic 35.0%
TACCTTATGGTTAGCAACAT+TGG + chr5.2:1129103-1129122 None:intergenic 35.0%
TCCAATCATAAACTCAGTAC+TGG - chr5.2:1129020-1129039 MS.gene21848:intron 35.0%
TGAAGAAAAGAGAGACAGAA+GGG + chr5.2:1130798-1130817 None:intergenic 35.0%
TGTAGTAGGAAAGAGTGATT+TGG + chr5.2:1128105-1128124 None:intergenic 35.0%
TTAGGCTTGTCTCTAATACA+TGG + chr5.2:1129480-1129499 None:intergenic 35.0%
TTATGGTTAGCAACATTGGT+TGG + chr5.2:1129099-1129118 None:intergenic 35.0%
! ACCAGTACTGAGTTTATGAT+TGG + chr5.2:1129024-1129043 None:intergenic 35.0%
! AGATCACAAATCTTTGAAGG+CGG - chr5.2:1131275-1131294 MS.gene21848:CDS 35.0%
! TTGAAATGTTGCATAATGGG+CGG - chr5.2:1130064-1130083 MS.gene21848:CDS 35.0%
!! ACATCTTTTATTTGCGAGAG+AGG - chr5.2:1130469-1130488 MS.gene21848:intron 35.0%
!! GCTCTTCTTCTAAAGTCTAA+AGG + chr5.2:1128202-1128221 None:intergenic 35.0%
!! GGTTCTAAGATGATTCTCTT+AGG - chr5.2:1129371-1129390 MS.gene21848:intron 35.0%
AAAAGGGTTACAAGCTAACG+AGG - chr5.2:1130318-1130337 MS.gene21848:intron 40.0%
AAAGGGGAATTGCCATTCTT+TGG + chr5.2:1129620-1129639 None:intergenic 40.0%
AATAAACAAACACACGGGGA+TGG + chr5.2:1129830-1129849 None:intergenic 40.0%
AATGCAAACTTGTGCAGCAA+GGG + chr5.2:1129001-1129020 None:intergenic 40.0%
ACATTTCAATGAAGGGGGAA+AGG + chr5.2:1130053-1130072 None:intergenic 40.0%
AGAAACAGCGAAGGAAGAAA+CGG + chr5.2:1128048-1128067 None:intergenic 40.0%
AGGAGTTAACTAGACCTATC+TGG - chr5.2:1129644-1129663 MS.gene21848:intron 40.0%
ATAAACAAACACACGGGGAT+GGG + chr5.2:1129829-1129848 None:intergenic 40.0%
ATAAATAGGGGTAGCAACTG+CGG - chr5.2:1128146-1128165 MS.gene21848:intron 40.0%
ATGGCAATTCCCCTTTCATA+AGG - chr5.2:1129624-1129643 MS.gene21848:intron 40.0%
ATTACTCTTTCCTTCAACCC+TGG + chr5.2:1128965-1128984 None:intergenic 40.0%
CGTACTGAGTGGCATAAATA+GGG - chr5.2:1128133-1128152 MS.gene21848:intron 40.0%
GAAGAAAAGAGAGACAGAAG+GGG + chr5.2:1130797-1130816 None:intergenic 40.0%
GAGGGTCATTTCAAATTTCG+AGG + chr5.2:1128528-1128547 None:intergenic 40.0%
GATACTGAGAAATCAGCAGT+TGG - chr5.2:1127966-1127985 MS.gene21848:intron 40.0%
GCGGATCTTGACAAAATATG+TGG + chr5.2:1131032-1131051 None:intergenic 40.0%
GGATACCACCTTAAGATCAA+TGG + chr5.2:1130867-1130886 None:intergenic 40.0%
GGTGGTGAAAACATTTGATG+TGG - chr5.2:1129770-1129789 MS.gene21848:intron 40.0%
GTACTGAGTGGCATAAATAG+GGG - chr5.2:1128134-1128153 MS.gene21848:intron 40.0%
GTCTTTCGTAATCACCAGAT+AGG + chr5.2:1129661-1129680 None:intergenic 40.0%
GTGTTGAGCTGTTGATATGT+TGG - chr5.2:1130137-1130156 MS.gene21848:CDS 40.0%
TATTCTATGTCCAGGGTTGA+AGG - chr5.2:1128952-1128971 MS.gene21848:intron 40.0%
TCGAACCATTGATCTTAAGG+TGG - chr5.2:1130859-1130878 MS.gene21848:intron 40.0%
TCGTACTGAGTGGCATAAAT+AGG - chr5.2:1128132-1128151 MS.gene21848:intron 40.0%
TCGTCTGTGCATCTGAAAAA+GGG - chr5.2:1130302-1130321 MS.gene21848:intron 40.0%
TCGTTGTCTCTTCATAGCTT+AGG + chr5.2:1128494-1128513 None:intergenic 40.0%
TCTCGAGTATTATGGCTTAC+TGG - chr5.2:1128846-1128865 MS.gene21848:intron 40.0%
TGAAGGAAAGAGTAATCCAC+AGG - chr5.2:1128969-1128988 MS.gene21848:intron 40.0%
TGAAGGCTATTCTATGTCCA+GGG - chr5.2:1128945-1128964 MS.gene21848:intron 40.0%
TGCCTGAGTAAACACTGAAA+GGG + chr5.2:1128645-1128664 None:intergenic 40.0%
TTCCCTTTCAGTGTTTACTC+AGG - chr5.2:1128640-1128659 MS.gene21848:CDS 40.0%
TTGAAGGCTATTCTATGTCC+AGG - chr5.2:1128944-1128963 MS.gene21848:intron 40.0%
! CCATCAATCCTTTTCCTACT+CGG + chr5.2:1129190-1129209 None:intergenic 40.0%
! GATCACAAATCTTTGAAGGC+GGG - chr5.2:1131276-1131295 MS.gene21848:CDS 40.0%
! GTTTTCTTAACCAGTGAACG+AGG + chr5.2:1129685-1129704 None:intergenic 40.0%
! TGAACTTGTTTGGCAGCTAT+AGG + chr5.2:1128014-1128033 None:intergenic 40.0%
!! ACTGCTGATTTCTCAGTATC+AGG + chr5.2:1127966-1127985 None:intergenic 40.0%
!! CTTTTATTTGCGAGAGAGGT+AGG - chr5.2:1130473-1130492 MS.gene21848:intron 40.0%
!! GTATCTTTAAGATGGGATGG+AGG - chr5.2:1130351-1130370 MS.gene21848:intron 40.0%
!! GTGACGGACAAAAATTTCTG+TGG + chr5.2:1128602-1128621 None:intergenic 40.0%
CCGAGTAGGAAAAGGATTGA+TGG - chr5.2:1129187-1129206 MS.gene21848:intron 45.0%
GAATGCAAACTTGTGCAGCA+AGG + chr5.2:1129002-1129021 None:intergenic 45.0%
GAGGTTGACTAGTGACTACA+TGG + chr5.2:1129166-1129185 None:intergenic 45.0%
GCGATCAAAACACAGCTATC+TGG + chr5.2:1129276-1129295 None:intergenic 45.0%
GTCGTCTGTGCATCTGAAAA+AGG - chr5.2:1130301-1130320 MS.gene21848:intron 45.0%
GTGCCTGAGTAAACACTGAA+AGG + chr5.2:1128646-1128665 None:intergenic 45.0%
GTGCTTGACTATGCTGTCAT+TGG - chr5.2:1130615-1130634 MS.gene21848:intron 45.0%
TAAACAAACACACGGGGATG+GGG + chr5.2:1129828-1129847 None:intergenic 45.0%
TATGCCACTCAGTACGAGAT+CGG + chr5.2:1128129-1128148 None:intergenic 45.0%
TGACAAAAAGTGGCTAGGCA+AGG - chr5.2:1130834-1130853 MS.gene21848:intron 45.0%
TGCACATTCTACAAGCAGAG+AGG + chr5.2:1130023-1130042 None:intergenic 45.0%
! GTTTCTTCCTTCGCTGTTTC+TGG - chr5.2:1128047-1128066 MS.gene21848:intron 45.0%
! GTTTTCACCACCAAACCACT+GGG + chr5.2:1129762-1129781 None:intergenic 45.0%
! TCCAAGAGAAGTGCAATCAG+CGG + chr5.2:1128078-1128097 None:intergenic 45.0%
! TGTTTTCACCACCAAACCAC+TGG + chr5.2:1129763-1129782 None:intergenic 45.0%
! TTCATTTTCCACTGCGACAC+AGG + chr5.2:1130743-1130762 None:intergenic 45.0%
! TTTGAGTATGTGTTGGCCAC+TGG - chr5.2:1129848-1129867 MS.gene21848:intron 45.0%
!! AATCCTTTTCCTACTCGGTG+AGG + chr5.2:1129185-1129204 None:intergenic 45.0%
!! TATCAGTGGCGGATCTTCTT+GGG - chr5.2:1130644-1130663 MS.gene21848:intron 45.0%
!! TTATCAGTGGCGGATCTTCT+TGG - chr5.2:1130643-1130662 MS.gene21848:intron 45.0%
!! ATATATATATAACAAATATT+TGG - chr5.2:1131000-1131019 MS.gene21848:CDS 5.0%
ACCGTGATTTCATAGAGGCG+AGG + chr5.2:1129589-1129608 None:intergenic 50.0%
ACGAAAGACTCCTCGTTCAC+TGG - chr5.2:1129672-1129691 MS.gene21848:intron 50.0%
ACTAGTCAACCTCACCGAGT+AGG - chr5.2:1129173-1129192 MS.gene21848:intron 50.0%
AGTACGAGATCGGGTGTAGT+AGG + chr5.2:1128119-1128138 None:intergenic 50.0%
ATGCCACTCAGTACGAGATC+GGG + chr5.2:1128128-1128147 None:intergenic 50.0%
ATGTTATGCCTGTGTCGCAG+TGG - chr5.2:1130732-1130751 MS.gene21848:intron 50.0%
CAACCTCACCGAGTAGGAAA+AGG - chr5.2:1129179-1129198 MS.gene21848:intron 50.0%
CAGTTGGTCACAGCATCACA+TGG - chr5.2:1127982-1128001 MS.gene21848:intron 50.0%
GACACCAAAGCTCTTAAGCC+AGG + chr5.2:1127900-1127919 None:intergenic 50.0%
GATGTTGCAACCCAGTGGTT+TGG - chr5.2:1129749-1129768 MS.gene21848:intron 50.0%
GCCTCGCCTCTATGAAATCA+CGG - chr5.2:1129585-1129604 MS.gene21848:intron 50.0%
GGAATCCCAAGTTCGAACCA+GGG - chr5.2:1129791-1129810 MS.gene21848:intron 50.0%
GGTTACAAGCTAACGAGGCA+GGG - chr5.2:1130323-1130342 MS.gene21848:intron 50.0%
GTGGAAAGGGTGACTACTTC+TGG - chr5.2:1129991-1130010 MS.gene21848:intron 50.0%
TCTTCGTCCTTCTGTCTCAG+AGG + chr5.2:1130384-1130403 None:intergenic 50.0%
TGGAATCCCAAGTTCGAACC+AGG - chr5.2:1129790-1129809 MS.gene21848:intron 50.0%
! CCAAGAGAAGTGCAATCAGC+GGG + chr5.2:1128077-1128096 None:intergenic 50.0%
! GTTTACTCAGGCACTGTAGC+TGG - chr5.2:1128652-1128671 MS.gene21848:CDS 50.0%
!! CAAGTCACCTCTGAGACAGA+AGG - chr5.2:1130374-1130393 MS.gene21848:intron 50.0%
ACACCCGATCTCGTACTGAG+TGG - chr5.2:1128122-1128141 MS.gene21848:intron 55.0%
ACCACTGGGTTGCAACATCG+AGG + chr5.2:1129748-1129767 None:intergenic 55.0%
ATGGGGAAATGTGGTGACCC+TGG + chr5.2:1129811-1129830 None:intergenic 55.0%
CATTGCGGAAAGAGGATGCG+AGG + chr5.2:1131179-1131198 None:intergenic 55.0%
CGCTCCTGGCTTAAGAGCTT+TGG - chr5.2:1127893-1127912 MS.gene21848:intron 55.0%
GGATCTTCTTGGGTGCCCAT+GGG - chr5.2:1130654-1130673 MS.gene21848:intron 55.0%
GGGTTACAAGCTAACGAGGC+AGG - chr5.2:1130322-1130341 MS.gene21848:intron 55.0%
TCCTCGATGTTGCAACCCAG+TGG - chr5.2:1129744-1129763 MS.gene21848:intron 55.0%
! CCCGCTGATTGCACTTCTCT+TGG - chr5.2:1128074-1128093 MS.gene21848:intron 55.0%
! GGTGACCCTGGTTCGAACTT+GGG + chr5.2:1129799-1129818 None:intergenic 55.0%
! GTTGCAACCCAGTGGTTTGG+TGG - chr5.2:1129752-1129771 MS.gene21848:intron 55.0%
! TGGTGACCCTGGTTCGAACT+TGG + chr5.2:1129800-1129819 None:intergenic 55.0%
!! GGGAGTACCAGAAACAGCGA+AGG + chr5.2:1128057-1128076 None:intergenic 55.0%
ATCAGATCTGGGGGGAGCCA+TGG + chr5.2:1130682-1130701 None:intergenic 60.0%
CACACGGGGATGGGGAAATG+TGG + chr5.2:1129820-1129839 None:intergenic 60.0%
CACTGTAGCTGGAGCTCGAG+AGG - chr5.2:1128663-1128682 MS.gene21848:CDS 60.0%
CGGATCTTCTTGGGTGCCCA+TGG - chr5.2:1130653-1130672 MS.gene21848:intron 60.0%
GACGATGGAATCGACGCTCC+TGG - chr5.2:1127879-1127898 MS.gene21848:intron 60.0%
GCAGCAAGGGCAAAGTCCTG+TGG + chr5.2:1128988-1129007 None:intergenic 60.0%
TTGGGTGCCCATGGGAGCCA+TGG - chr5.2:1130662-1130681 MS.gene21848:intron 65.0%
GGGGGAGCCATGGCTCCCAT+GGG + chr5.2:1130672-1130691 None:intergenic 70.0%
GGGGGGAGCCATGGCTCCCA+TGG + chr5.2:1130673-1130692 None:intergenic 75.0%


Chromosome Type Strat End Strand Name
chr5.2 gene 1127825 1131302 1127825 ID=MS.gene21848
chr5.2 mRNA 1127825 1131302 1127825 ID=MS.gene21848.t1;Parent=MS.gene21848
chr5.2 exon 1130950 1131302 1130950 ID=MS.gene21848.t1.exon1;Parent=MS.gene21848.t1
chr5.2 CDS 1130950 1131302 1130950 ID=cds.MS.gene21848.t1;Parent=MS.gene21848.t1
chr5.2 exon 1130399 1130476 1130399 ID=MS.gene21848.t1.exon2;Parent=MS.gene21848.t1
chr5.2 CDS 1130399 1130476 1130399 ID=cds.MS.gene21848.t1;Parent=MS.gene21848.t1
chr5.2 exon 1130008 1130161 1130008 ID=MS.gene21848.t1.exon3;Parent=MS.gene21848.t1
chr5.2 CDS 1130008 1130161 1130008 ID=cds.MS.gene21848.t1;Parent=MS.gene21848.t1
chr5.2 exon 1128637 1128804 1128637 ID=MS.gene21848.t1.exon4;Parent=MS.gene21848.t1
chr5.2 CDS 1128637 1128804 1128637 ID=cds.MS.gene21848.t1;Parent=MS.gene21848.t1
chr5.2 exon 1127825 1127887 1127825 ID=MS.gene21848.t1.exon5;Parent=MS.gene21848.t1
chr5.2 CDS 1127825 1127887 1127825 ID=cds.MS.gene21848.t1;Parent=MS.gene21848.t1
Gene Sequence

>MS.gene21848

ATGGAAGAGGGTAACATTAACAATAAGAGAGGAGGAAGAATTCTGATAACAAACGACGATGGAATCGACGCTCCTGGCTTAAGAGCTTTGGTGTCATCATTAGTGAATAGTAATCTTTTCAATATCTTTGTGTGTGCTCCTGATACTGAGAAATCAGCAGTTGGTCACAGCATCACATGGCTTCATCCTATAGCTGCCAAACAAGTTCATATTCATGGAACCGTTTCTTCCTTCGCTGTTTCTGGTACTCCCGCTGATTGCACTTCTCTTGGAATTTCCAAATCACTCTTTCCTACTACACCCGATCTCGTACTGAGTGGCATAAATAGGGGTAGCAACTGCGGCTATCATATGTATGTACCACCATTTTATCACCTTTAGACTTTAGAAGAAGAGCTTATTTAGAATTTTCATCTCATGATATAATATAACCACTTATATTGTTTGTTAGTGTTGTAGTTTTGTAAACTAGCTTATGACATGTCCGTAAATTGTTTTTACTTATTTCTATAAATTAGATTGTAATAATTATTTGCCCAAAGAAATAATTTACATTGCTTACAATCTTTCTCGCAGTCAATAAATTATTTTGTATGTCATATCAAGTGATGTAAGCGTACCTGTTAAAAATTGTTCTATTTATTTCAAATGACAAAGGATTATCATCCTAAGCTATGAAGAGACAACGATGATTTCATTTCCTCGAAATTTGAAATGACCCTCTTATGATTTATACTATTTTTATTTTTGCTAAGGTTTATTCAGTAGTTATTACCACAGAAATTTTTGTCCGTCACTGTTTGTGTAATTAGTAGTTCCCTTTCAGTGTTTACTCAGGCACTGTAGCTGGAGCTCGAGAGGCCTTCTTCAACAATATCCCTTCTATCTCCATTTCATATGACTGGTATGTTATCGACATGTTTCTGTTGCAATATCGCTAGATCTGTCTTCCATATTTGTTAAGTTGAACTTTTGAAACCAATTAATCAGCTTATAATTGTAACTGATAACTTAATGAATGTCTCGAGTATTATGGCTTACTGGCTTTATAAGTTATTTCAGAAATCTTTAATCTATATCCTTTTATAGCAATAGCTTTTCATTATCATGTTTGACCATTTGAAGGCTATTCTATGTCCAGGGTTGAAGGAAAGAGTAATCCACAGGACTTTGCCCTTGCTGCACAAGTTTGCATTCCAATCATAAACTCAGTACTGGTTGCGATAAAGAAACAAAGCTACCCTGGAAGATGTTTTTTGAATATAGACGTGCCAACCAATGTTGCTAACCATAAGGTATATTGTAATAAGTTTTGCAATTGTGATGTTGAATGATGATCCATGTAGTCACTAGTCAACCTCACCGAGTAGGAAAAGGATTGATGGTTGTTCATATTTTTGTGTATGTTTAGCTTGTGAGAATTATTATTATGATTCTATTATATATAGCCAGATAGCTGTGTTTTGATCGCTTAACATAACATAAATTAATGCTGTGATGGAATAAGCATAATACATGATAAGGAGATTTATTCATAATATATGCTGGTTCTAAGATGATTCTCTTAGGAATTATCTTTTAAATTTTTTATTGTTACTTTAGGTAATTTGACAGGCATTATATTAGGACTATAGCTCTATTAATAGGAGACACCATGTATTAGAGACAAGCCTAAGAAAATTGATTATATTCTATCCAAGTTTTATTGTGCGTAAGTTCCAGAAATTGTCATATAAATCTTGTTTGTTTGCTAGAATGTTGCCTCGCCTCTATGAAATCACGGTAGTTATATCCAAAGAATGGCAATTCCCCTTTCATAAGGAGTTAACTAGACCTATCTGGTGATTACGAAAGACTCCTCGTTCACTGGTTAAGAAAACGTTGTCTTGTGTGTTATGATAATTTGTGGGATTTGTTTGTCCTCGATGTTGCAACCCAGTGGTTTGGTGGTGAAAACATTTGATGTGGAATCCCAAGTTCGAACCAGGGTCACCACATTTCCCCATCCCCGTGTGTTTGTTTATTTGAGTATGTGTTGGCCACTGGTTGAATAGCAAATTTTTATCCTTGTGGAATTTATTGTTTTGGATAAATGTTATTTGTCTGTGCAAAAACAAAAAAAGCTTCCTGATATCATGACAATATATACTACCGCATTGTAAATTAGTGGAAAGGGTGACTACTTCTGGATGAAGCCTCTCTGCTTGTAGAATGTGCACATCTGACCTTTCCCCCTTCATTGAAATGTTGCATAATGGGCGGCCCTTGTTACAAAAAGATGCTGTTTTAACATTTGCTTTGTTGTCATTGAAGTGTTGAGCTGTTGATATGTTGGTTTGATTATCTGCCTTCTGTATTTTCATTTAATATCCTACATATTGAAGTTTACATTTGTATTCAAAGGCTTGCTATTGCTGACTTTACTACAATAATATATTGCTCTGTCATGCAATCTTCTGTAACTAATTTATTGGTTGTCGTCTGTGCATCTGAAAAAGGGTTACAAGCTAACGAGGCAGGGTAAAAGTATCTTTAAGATGGGATGGAGGCAAGTCACCTCTGAGACAGAAGGACGAAGAATGTCGTCTGATATGACCAATACAGATACACCTAAAAACTTTGGTACATCATCTGCATCACCTGAACATCTTTTATTTGCGAGAGAGGTAGGCTCATTTACTTTCTTTCTAATTGACTTGAAGTCTCGATATTTCATTTGTATGTAGCATATTGTCTAATACTTTCGGATGTACTGCTTCGATTGTTATAAGTTCTACACCGCTTAATATTGTGCTTGACTATGCTGTCATTGGACTTATTATCAGTGGCGGATCTTCTTGGGTGCCCATGGGAGCCATGGCTCCCCCCAGATCTGATATAAATACTATATTACACTGTTATTATCAAATGTTATGCCTGTGTCGCAGTGGAAAATGAAGTTGTTTTGCACTATTAGGTCGTGAGTTCGACCCCTTCTGTCTCTCTTTTCTTCATATTTTTATTTGAATTTGACAAAAAGTGGCTAGGCAAGGATTCGAACCATTGATCTTAAGGTGGTATCCAAGCAATATAAACAACAACACCACATATCAACTTGTGTTCATTGTGTGAAACAACTTTTATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATAACAAATATTTGGCTCCCCCCACATATTTTGTCAAGATCCGCCACTGCTTATTATAAACAGAGTAGAGTGAAGATTGTAAGTATTTGATTTTATCAACTTCTGAAATAGATGCCAAGTTATATGGAAGAACTAAAAAACTTACTTGGATGGACATTTCGTAATCTTCCTCGCATCCTCTTTCCGCAATGAATAATAATGTAGTTTTTTTTTTTTCTTCAAACTTTTGTAGGTAAGAGGCGCCCTACGTGATTATGATGATGATACAGATCACAAATCTTTGAAGGCGGGCTAT
Protein sequence

>MS.gene21848.t1

MEEGNINNKRGGRILITNDDGIDAPGLRALVSSLVNSNLFNIFVCAPDTEKSAVGHSITWLHPIAAKQVHIHGTVSSFAVSGTPADCTSLGISKSLFPTTPDLVLSGINRGSNCGYHIVYSGTVAGAREAFFNNIPSISISYDWVEGKSNPQDFALAAQVCIPIINSVLVAIKKQSYPGRCFLNIDVPTNVANHKGYKLTRQGKSIFKMGWRQVTSETEGRRMSSDMTNTDTPKNFGTSSASPEHLLFAREVRGALRDYDDDTDHKSLKAGY