AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene85230

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene85230.t1 MTR_2g099720 97.347 377 10 0 1 377 1 377 0.0 758
MS.gene85230.t1 MTR_5g006290 36.036 333 171 7 45 372 3 298 3.37e-67 215
MS.gene85230.t1 MTR_1g104500 35.152 330 177 5 45 372 3 297 6.29e-66 211
MS.gene85230.t1 MTR_5g006290 45.755 212 113 2 45 255 3 213 5.86e-61 197
MS.gene85230.t1 MTR_5g006300 43.318 217 108 5 47 255 3 212 1.72e-50 171
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene85230.t1 AT1G72880 62.992 381 133 6 2 377 8 385 6.30e-168 474
MS.gene85230.t1 AT1G72880 62.992 381 133 6 2 377 8 385 6.30e-168 474
MS.gene85230.t1 AT4G14930 35.692 325 175 6 50 372 13 305 1.24e-61 201
MS.gene85230.t1 AT4G14930 47.087 206 106 2 50 253 13 217 5.14e-60 196

Find 97 sgRNAs with CRISPR-Local

Find 180 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAAATATTTCTTCACTTTAT+TGG 0.174808 2.4:+4569516 None:intergenic
TTTAGGGATAAATCTGTTTC+TGG 0.178353 2.4:-4571356 MS.gene85230:intron
TTCTTCACTTTATTGGTATC+AGG 0.242064 2.4:+4569523 None:intergenic
TGGATTTCTGCTGTGATTCC+TGG 0.252539 2.4:-4568927 MS.gene85230:CDS
AATTCGATAGGGTTAGGATT+AGG 0.265411 2.4:+4571618 None:intergenic
TCCTGTGGATTGTGTTTCAT+TGG 0.274471 2.4:-4571111 MS.gene85230:CDS
CGTTGATAAATGCAGCTATC+AGG 0.280646 2.4:-4570179 MS.gene85230:CDS
ATTCGATAGGGTTAGGATTA+GGG 0.300748 2.4:+4571619 None:intergenic
CTATCCAACTGGTCATTTCT+TGG 0.301621 2.4:-4569799 MS.gene85230:CDS
AGGTTATTAGTGGGATTAAT+CGG 0.308990 2.4:-4570636 MS.gene85230:CDS
GCTTGTTCTACTCAAGTTAA+TGG 0.321601 2.4:-4571302 MS.gene85230:CDS
TGTGTTTCATTGGCTTTATC+CGG 0.331048 2.4:-4571101 MS.gene85230:CDS
TTTGAAACAGCTAACCAGTT+TGG 0.357202 2.4:+4569828 None:intergenic
TTGGAGATTATTGAATCAAC+TGG 0.357915 2.4:-4569559 MS.gene85230:CDS
GCTGTTTGTGAGCAAGATAA+TGG 0.364803 2.4:+4571581 None:intergenic
TTGTTGCTCAAAGGTGATCT+TGG 0.382717 2.4:+4570105 None:intergenic
CATTATCAATATCATTAAAC+TGG 0.385947 2.4:-4570342 MS.gene85230:intron
TTCAAACAACAATTTCTGAT+TGG 0.386487 2.4:-4568947 MS.gene85230:CDS
TCATCGAATTCGATAGGGTT+AGG 0.387109 2.4:+4571612 None:intergenic
GAGAAGTGCTTCTTGAAGAT+TGG 0.399004 2.4:+4571710 None:intergenic
TAATAAATGCTAACCAAAAC+AGG 0.405501 2.4:+4571056 None:intergenic
GTGTTTCATTGGCTTTATCC+GGG 0.415974 2.4:-4571100 MS.gene85230:CDS
CTGTTTGTGAGCAAGATAAT+GGG 0.422044 2.4:+4571582 None:intergenic
GGTTATTAGTGGGATTAATC+GGG 0.422540 2.4:-4570635 MS.gene85230:CDS
TTCGTGGTCAAAGCCTGTTT+TGG 0.423001 2.4:-4571069 MS.gene85230:intron
TGAAGATTGGAAACGAGGCC+CGG 0.439122 2.4:+4571723 None:intergenic
TGTGAGCAAGATAATGGGTT+TGG 0.440916 2.4:+4571587 None:intergenic
AAGTCAAGAAACTGATTTCA+AGG 0.442158 2.4:-4570223 MS.gene85230:CDS
TTTGAATTTCAGGTTATTAG+TGG 0.444025 2.4:-4570646 MS.gene85230:intron
AGAAATAGATGACGATGATC+TGG 0.446591 2.4:-4569199 MS.gene85230:CDS
GAAGATTGGAAACGAGGCCC+GGG 0.453439 2.4:+4571724 None:intergenic
TTTAATGATATTGATAATGA+TGG 0.457599 2.4:+4570346 None:intergenic
GGTCTTCAGTTTGCACAGCT+TGG 0.463528 2.4:-4569756 MS.gene85230:CDS
ATTGAATCAACTGGAGCTGC+AGG 0.468057 2.4:-4569550 MS.gene85230:CDS
GCCAATCCGCAAGGTGGCCT+AGG 0.476369 2.4:-4569777 MS.gene85230:CDS
AGCTATCAGGGATGCTGAAA+AGG 0.478095 2.4:-4570166 MS.gene85230:CDS
TGTGGAGATCCACTCCAAAC+TGG 0.479831 2.4:-4569842 MS.gene85230:CDS
TCAAATAGCCGCTATCCAAC+TGG 0.485041 2.4:-4569810 MS.gene85230:CDS
GGATCTCCACATACTTTGCT+TGG 0.489007 2.4:+4569854 None:intergenic
TCGTTTCAAGATGATGCCCC+CGG 0.496104 2.4:-4571742 None:intergenic
AGGATTAGGGTTATCATCGT+TGG 0.497932 2.4:+4571632 None:intergenic
AAAATGCTCCAGGTTCTACT+CGG 0.500819 2.4:-4570410 MS.gene85230:intron
TTGAATTTCAGGTTATTAGT+GGG 0.502752 2.4:-4570645 MS.gene85230:intron
CGCAAACATGGACATTGTAA+AGG 0.507879 2.4:+4571502 None:intergenic
TTGGATTCATCGAATTCGAT+AGG 0.510175 2.4:+4571606 None:intergenic
AATTGACCAAGCAAAGTATG+TGG 0.510381 2.4:-4569860 MS.gene85230:CDS
ACCTAGGCCACCTTGCGGAT+TGG 0.512987 2.4:+4569776 None:intergenic
TGAATATCGCTCTCAATATG+AGG 0.515443 2.4:+4568966 None:intergenic
AAATGCTCCAGGTTCTACTC+GGG 0.515947 2.4:-4570409 MS.gene85230:intron
TTTGATTCATCTTGTTGTGA+AGG 0.524748 2.4:+4571672 None:intergenic
ATTAGGGTTATCATCGTTGG+TGG 0.527462 2.4:+4571635 None:intergenic
TATATCATGTTACAGGAAGA+AGG 0.527852 2.4:-4570250 MS.gene85230:intron
TCAGGGTGCAGCTCGTCGGT+TGG 0.528374 2.4:-4569599 MS.gene85230:intron
TTGTTCAGGGTGCAGCTCGT+CGG 0.533664 2.4:-4569603 MS.gene85230:intron
GTTGATAAATGCAGCTATCA+GGG 0.535579 2.4:-4570178 MS.gene85230:CDS
TTGAGTAGAACAAGCTTCCA+CGG 0.536699 2.4:+4571310 None:intergenic
GAAGAACGAGAGCTTCAACT+AGG 0.548339 2.4:+4571529 None:intergenic
ATATGAGGAGAAAGAGATAC+CGG 0.550193 2.4:+4568981 None:intergenic
TTACACACCTTGTTGCTCAA+AGG 0.553532 2.4:+4570096 None:intergenic
TGGATTCATCGAATTCGATA+GGG 0.554963 2.4:+4571607 None:intergenic
TGTTTAATTTACTGTCCACC+AGG 0.555593 2.4:+4568909 None:intergenic
GGGTTATCATCGTTGGTGGA+AGG 0.558302 2.4:+4571639 None:intergenic
GGTTGTTGCTGGAGCTAGGG+AGG 0.559179 2.4:-4570386 MS.gene85230:CDS
GATTACAGAGCACTTGAAAG+TGG 0.567031 2.4:-4569177 MS.gene85230:CDS
ATCATCGTTGGTGGAAGGAA+CGG 0.568122 2.4:+4571644 None:intergenic
ATTTCTGCTGTGATTCCTGG+TGG 0.569344 2.4:-4568924 MS.gene85230:CDS
CATTTCTTGGCCAATCCGCA+AGG 0.573836 2.4:-4569786 MS.gene85230:CDS
AAGATTGGAAACGAGGCCCG+GGG 0.581766 2.4:+4571725 None:intergenic
GAAACAGGTTGCAGTGACTC+CGG 0.585349 2.4:-4569000 MS.gene85230:intron
AAGATCACCTTTGAGCAACA+AGG 0.596181 2.4:-4570103 MS.gene85230:intron
GCCAATGAAACACAATCCAC+AGG 0.598467 2.4:+4571110 None:intergenic
TAATAATTCAGGGACTCCTG+TGG 0.599995 2.4:-4571126 MS.gene85230:intron
ACGATTGAGGAACGCAAACA+TGG 0.602918 2.4:+4571490 None:intergenic
CTTCTTGAAGATTGGAAACG+AGG 0.607887 2.4:+4571718 None:intergenic
CTGATAGCTGCATTTATCAA+CGG 0.609132 2.4:+4570180 None:intergenic
ACCTGAAATCTCAAAAGCAG+TGG 0.609300 2.4:+4571277 None:intergenic
TGTTTCATTGGCTTTATCCG+GGG 0.611446 2.4:-4571099 MS.gene85230:CDS
AACAGCTAACCAGTTTGGAG+TGG 0.612572 2.4:+4569833 None:intergenic
ATCTTGCTCACAAACAGCGA+TGG 0.615557 2.4:-4571576 MS.gene85230:CDS
AATGCTCCAGGTTCTACTCG+GGG 0.618010 2.4:-4570408 MS.gene85230:intron
GAAGAAATATTTCAGACTAG+AGG 0.618268 2.4:-4569506 MS.gene85230:intron
GTTATTAGTGGGATTAATCG+GGG 0.623644 2.4:-4570634 MS.gene85230:CDS
TTACAGCTGCAGAGGCATCT+CGG 0.632794 2.4:+4569733 None:intergenic
TTGGCCAAGAAATGACCAGT+TGG 0.632927 2.4:+4569795 None:intergenic
TAGGATACTTACAGCTGCAG+AGG 0.633261 2.4:+4569725 None:intergenic
AGCGATGGAGTTGAATCACC+AGG 0.636526 2.4:-4571561 MS.gene85230:CDS
TCAACTAGGTGTGTAAGACC+TGG 0.638164 2.4:+4571543 None:intergenic
TGACCACGAAAACAACGCCC+CGG 0.642921 2.4:+4571082 None:intergenic
AGAAATGACCAGTTGGATAG+CGG 0.646911 2.4:+4569802 None:intergenic
TTCTTGGCCAATCCGCAAGG+TGG 0.656618 2.4:-4569783 MS.gene85230:CDS
ATGCTCCAGGTTCTACTCGG+GGG 0.678166 2.4:-4570407 MS.gene85230:intron
TGTTACGATTCGAGAAACCG+TGG 0.686133 2.4:-4571327 MS.gene85230:CDS
TGAAGACCTAGGCCACCTTG+CGG 0.693929 2.4:+4569771 None:intergenic
ATTAATCGGGGATCAAGCTG+CGG 0.696145 2.4:-4570622 MS.gene85230:CDS
GCTAGGGAGGCATTATTATG+CGG 0.712293 2.4:-4570373 MS.gene85230:CDS
GCTGTGCAAACTGAAGACCT+AGG 0.716901 2.4:+4569760 None:intergenic
AGATTGGAAACGAGGCCCGG+GGG 0.763136 2.4:+4571726 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAGTTATTTATAATAATTCA+GGG - chr2.4:4569513-4569532 MS.gene85230:CDS 10.0%
!! TTAGTTATTTATAATAATTC+AGG - chr2.4:4569512-4569531 MS.gene85230:CDS 10.0%
!!! AATTTGATTTTTTTTTTTTC+CGG - chr2.4:4570591-4570610 MS.gene85230:intron 10.0%
!!! TAAAATTTTAAAATTGATTC+TGG - chr2.4:4569703-4569722 MS.gene85230:intron 10.0%
!!! TAAATTTTTGATTTTTTTGT+TGG - chr2.4:4569214-4569233 MS.gene85230:CDS 10.0%
!! GTAAAATGATTTATTATGTT+TGG - chr2.4:4569743-4569762 MS.gene85230:CDS 15.0%
!!! ATCTGAAATTTTTGTTTTTT+TGG - chr2.4:4569422-4569441 MS.gene85230:intron 15.0%
!!! TCTGAAATTTTTGTTTTTTT+GGG - chr2.4:4569423-4569442 MS.gene85230:intron 15.0%
!!! TTTAATGATATTGATAATGA+TGG + chr2.4:4570306-4570325 None:intergenic 15.0%
!! AACAAAATCAAATTACAAGA+CGG + chr2.4:4570141-4570160 None:intergenic 20.0%
!! CATTATCAATATCATTAAAC+TGG - chr2.4:4570307-4570326 MS.gene85230:intron 20.0%
!! CTTGATGTTAATATCTTTAT+GGG - chr2.4:4571563-4571582 MS.gene85230:CDS 20.0%
!! GAAATATTTCTTCACTTTAT+TGG + chr2.4:4571136-4571155 None:intergenic 20.0%
!! TCTTGATGTTAATATCTTTA+TGG - chr2.4:4571562-4571581 MS.gene85230:CDS 20.0%
!! TGAAATACAAATATAACTCA+TGG + chr2.4:4571349-4571368 None:intergenic 20.0%
!!! AATATTCAGAAAGCAATTTT+TGG + chr2.4:4570518-4570537 None:intergenic 20.0%
!!! ATATTCAGAAAGCAATTTTT+GGG + chr2.4:4570517-4570536 None:intergenic 20.0%
! ATATCATTAAACTGGTAAGT+TGG - chr2.4:4570315-4570334 MS.gene85230:intron 25.0%
! CAAACACACAATTAATTTAC+GGG + chr2.4:4569636-4569655 None:intergenic 25.0%
! CCAAACACACAATTAATTTA+CGG + chr2.4:4569637-4569656 None:intergenic 25.0%
! GAATAAAAAACTCCAAATTC+TGG - chr2.4:4569822-4569841 MS.gene85230:CDS 25.0%
! TAATAAATGCTAACCAAAAC+AGG + chr2.4:4569596-4569615 None:intergenic 25.0%
! TTCAAACAACAATTTCTGAT+TGG - chr2.4:4571702-4571721 MS.gene85230:CDS 25.0%
! TTCATGTATTGTACAAAATC+TGG - chr2.4:4570652-4570671 MS.gene85230:intron 25.0%
!! ATTTTTGTTAGTTGCTGAAA+TGG - chr2.4:4569264-4569283 MS.gene85230:intron 25.0%
!!! ATAAATCTGAGTTCTGAAAT+TGG - chr2.4:4570093-4570112 MS.gene85230:intron 25.0%
!!! TTGAATTTCAGGTTATTAGT+GGG - chr2.4:4570004-4570023 MS.gene85230:intron 25.0%
!!! TTTGAATTTCAGGTTATTAG+TGG - chr2.4:4570003-4570022 MS.gene85230:intron 25.0%
AAGTTGCTAACTTATGGTTA+GGG - chr2.4:4570061-4570080 MS.gene85230:intron 30.0%
ACATGTAAGTTGCTAACTTA+TGG - chr2.4:4570055-4570074 MS.gene85230:intron 30.0%
AGGTTATTAGTGGGATTAAT+CGG - chr2.4:4570013-4570032 MS.gene85230:intron 30.0%
ATTTCAGACTAGAGGTAAAT+AGG - chr2.4:4571151-4571170 MS.gene85230:intron 30.0%
CAAAATCAATTCTATACCTC+TGG - chr2.4:4569906-4569925 MS.gene85230:intron 30.0%
CATGATGTGATTTGAATTTC+AGG - chr2.4:4569993-4570012 MS.gene85230:intron 30.0%
CCGTAAATTAATTGTGTGTT+TGG - chr2.4:4569634-4569653 MS.gene85230:intron 30.0%
GAAGAAATATTTCAGACTAG+AGG - chr2.4:4571143-4571162 MS.gene85230:intron 30.0%
GTACAAAATCTGGTATTCTA+GGG - chr2.4:4570662-4570681 MS.gene85230:intron 30.0%
TAAGTTGCTAACTTATGGTT+AGG - chr2.4:4570060-4570079 MS.gene85230:intron 30.0%
TATATCATGTTACAGGAAGA+AGG - chr2.4:4570399-4570418 MS.gene85230:CDS 30.0%
TCAAATTACAAGACGGAAAA+TGG + chr2.4:4570134-4570153 None:intergenic 30.0%
TGTACAAAATCTGGTATTCT+AGG - chr2.4:4570661-4570680 MS.gene85230:intron 30.0%
TTCTTCACTTTATTGGTATC+AGG + chr2.4:4571129-4571148 None:intergenic 30.0%
TTGGAGATTATTGAATCAAC+TGG - chr2.4:4571090-4571109 MS.gene85230:CDS 30.0%
TTTAGGGATAAATCTGTTTC+TGG - chr2.4:4569293-4569312 MS.gene85230:intron 30.0%
! AAGTCAAGAAACTGATTTCA+AGG - chr2.4:4570426-4570445 MS.gene85230:intron 30.0%
! GATATTTTCTGTCTATTCTC+AGG - chr2.4:4570973-4570992 MS.gene85230:intron 30.0%
! GTATGTTTGGTTTAACTTCT+GGG + chr2.4:4569948-4569967 None:intergenic 30.0%
! TATGTTTGGTTTAACTTCTG+GGG + chr2.4:4569947-4569966 None:intergenic 30.0%
! TGTATGTTTGGTTTAACTTC+TGG + chr2.4:4569949-4569968 None:intergenic 30.0%
! TTTAAAGCCCTGTCAATAAA+AGG + chr2.4:4570771-4570790 None:intergenic 30.0%
! TTTGATTCATCTTGTTGTGA+AGG + chr2.4:4568980-4568999 None:intergenic 30.0%
!! ATGCTCTAGAGATTATGAAT+TGG - chr2.4:4570371-4570390 MS.gene85230:CDS 30.0%
!! TTGAATCCATGTTTTGTTCA+GGG - chr2.4:4571033-4571052 MS.gene85230:intron 30.0%
!!! TATACCTCTGGAATCAATTT+TGG - chr2.4:4569918-4569937 MS.gene85230:intron 30.0%
AAAAAAAGAACGCACGATTG+AGG + chr2.4:4569175-4569194 None:intergenic 35.0%
AATTCGATAGGGTTAGGATT+AGG + chr2.4:4569034-4569053 None:intergenic 35.0%
AATTGACCAAGCAAAGTATG+TGG - chr2.4:4570789-4570808 MS.gene85230:intron 35.0%
AGAAATAGATGACGATGATC+TGG - chr2.4:4571450-4571469 MS.gene85230:intron 35.0%
ATATGAGGAGAAAGAGATAC+CGG + chr2.4:4571671-4571690 None:intergenic 35.0%
ATTCGATAGGGTTAGGATTA+GGG + chr2.4:4569033-4569052 None:intergenic 35.0%
CACATGTTTGTATGTAGTGT+AGG + chr2.4:4570946-4570965 None:intergenic 35.0%
CAGCACTATTAGTGTATGTT+TGG + chr2.4:4569961-4569980 None:intergenic 35.0%
CTCTACACATCTCTATAACA+AGG + chr2.4:4570197-4570216 None:intergenic 35.0%
CTGATAGCTGCATTTATCAA+CGG + chr2.4:4570472-4570491 None:intergenic 35.0%
CTGTTTGTGAGCAAGATAAT+GGG + chr2.4:4569070-4569089 None:intergenic 35.0%
GCTTGTTCTACTCAAGTTAA+TGG - chr2.4:4569347-4569366 MS.gene85230:intron 35.0%
GGTATCGTATATCATGTTAC+AGG - chr2.4:4570392-4570411 MS.gene85230:CDS 35.0%
GGTTATTAGTGGGATTAATC+GGG - chr2.4:4570014-4570033 MS.gene85230:intron 35.0%
GTACAGAAGACAACAATTTG+TGG - chr2.4:4571266-4571285 MS.gene85230:intron 35.0%
GTTATTAGTGGGATTAATCG+GGG - chr2.4:4570015-4570034 MS.gene85230:intron 35.0%
TATAACAAGGCAGCAAATTC+TGG + chr2.4:4570184-4570203 None:intergenic 35.0%
TCTAACTAGAAGCCAGAATT+TGG + chr2.4:4569837-4569856 None:intergenic 35.0%
TGAATATCGCTCTCAATATG+AGG + chr2.4:4571686-4571705 None:intergenic 35.0%
TGGATTCATCGAATTCGATA+GGG + chr2.4:4569045-4569064 None:intergenic 35.0%
TTACTGCATTGTGCAAAATC+TGG - chr2.4:4570620-4570639 MS.gene85230:CDS 35.0%
TTGGATTCATCGAATTCGAT+AGG + chr2.4:4569046-4569065 None:intergenic 35.0%
! AGTGGTATCCTTTTATTGAC+AGG - chr2.4:4570760-4570779 MS.gene85230:intron 35.0%
! CTACTTGTACCTTCAGTTTT+TGG - chr2.4:4571414-4571433 MS.gene85230:intron 35.0%
! GTGGTATCCTTTTATTGACA+GGG - chr2.4:4570761-4570780 MS.gene85230:intron 35.0%
! GTTGATAAATGCAGCTATCA+GGG - chr2.4:4570471-4570490 MS.gene85230:intron 35.0%
! TCTGGCTAAAAATGAGTTGA+AGG - chr2.4:4569721-4569740 MS.gene85230:intron 35.0%
! TTTGAAACAGCTAACCAGTT+TGG + chr2.4:4570824-4570843 None:intergenic 35.0%
!! ACCTGGAGCATTTTAAAGAA+TGG + chr2.4:4570233-4570252 None:intergenic 35.0%
!! AGTTGGTGATTCTATACATC+TGG - chr2.4:4570332-4570351 MS.gene85230:intron 35.0%
!! CTTGAATCCATGTTTTGTTC+AGG - chr2.4:4571032-4571051 MS.gene85230:intron 35.0%
!! TGTGTTTCATTGGCTTTATC+CGG - chr2.4:4569548-4569567 MS.gene85230:CDS 35.0%
AAAATGCTCCAGGTTCTACT+CGG - chr2.4:4570239-4570258 MS.gene85230:intron 40.0%
AAGATCACCTTTGAGCAACA+AGG - chr2.4:4570546-4570565 MS.gene85230:intron 40.0%
ACCTGAAATCTCAAAAGCAG+TGG + chr2.4:4569375-4569394 None:intergenic 40.0%
AGAAATGACCAGTTGGATAG+CGG + chr2.4:4570850-4570869 None:intergenic 40.0%
AGGATTAGGGTTATCATCGT+TGG + chr2.4:4569020-4569039 None:intergenic 40.0%
ATGAATCCGTCAGAATGAGA+AGG + chr2.4:4571615-4571634 None:intergenic 40.0%
ATTAGGGTTATCATCGTTGG+TGG + chr2.4:4569017-4569036 None:intergenic 40.0%
CACAATGCAGTAAAACATGC+CGG + chr2.4:4570613-4570632 None:intergenic 40.0%
CGCAAACATGGACATTGTAA+AGG + chr2.4:4569150-4569169 None:intergenic 40.0%
CTATCCAACTGGTCATTTCT+TGG - chr2.4:4570850-4570869 MS.gene85230:intron 40.0%
CTTCTTGAAGATTGGAAACG+AGG + chr2.4:4568934-4568953 None:intergenic 40.0%
GAATGAGAAGGGAGCAAAAT+TGG + chr2.4:4571603-4571622 None:intergenic 40.0%
GACTAGAGGTAAATAGGAGT+AGG - chr2.4:4571157-4571176 MS.gene85230:intron 40.0%
GCTGTTTGTGAGCAAGATAA+TGG + chr2.4:4569071-4569090 None:intergenic 40.0%
GGCTACGCAGAAAAAGAATT+TGG - chr2.4:4571071-4571090 MS.gene85230:CDS 40.0%
TAAATAGGAGTAGGAGGATG+AGG - chr2.4:4571166-4571185 MS.gene85230:intron 40.0%
TAATAATTCAGGGACTCCTG+TGG - chr2.4:4569523-4569542 MS.gene85230:CDS 40.0%
TAGAGGTAAATAGGAGTAGG+AGG - chr2.4:4571160-4571179 MS.gene85230:intron 40.0%
TCATCGAATTCGATAGGGTT+AGG + chr2.4:4569040-4569059 None:intergenic 40.0%
TGAATCCGTCAGAATGAGAA+GGG + chr2.4:4571614-4571633 None:intergenic 40.0%
TTACACACCTTGTTGCTCAA+AGG + chr2.4:4570556-4570575 None:intergenic 40.0%
TTGAGTAGAACAAGCTTCCA+CGG + chr2.4:4569342-4569361 None:intergenic 40.0%
TTGTTGCTCAAAGGTGATCT+TGG + chr2.4:4570547-4570566 None:intergenic 40.0%
! CGTTGATAAATGCAGCTATC+AGG - chr2.4:4570470-4570489 MS.gene85230:intron 40.0%
! GATTACAGAGCACTTGAAAG+TGG - chr2.4:4571472-4571491 MS.gene85230:intron 40.0%
! GTAGCCAAAATTGATTCCAG+AGG + chr2.4:4569925-4569944 None:intergenic 40.0%
! TCCTGTGGATTGTGTTTCAT+TGG - chr2.4:4569538-4569557 MS.gene85230:CDS 40.0%
! TGCTGTTTATACTGATGCAG+TGG - chr2.4:4570742-4570761 MS.gene85230:intron 40.0%
!! GAGAAGTGCTTCTTGAAGAT+TGG + chr2.4:4568942-4568961 None:intergenic 40.0%
!! GCCATTCTTTAAAATGCTCC+AGG - chr2.4:4570229-4570248 MS.gene85230:CDS 40.0%
!! GCTGAAATGGTGTTGCTTTA+GGG - chr2.4:4569277-4569296 MS.gene85230:intron 40.0%
!! GTGTTTCATTGGCTTTATCC+GGG - chr2.4:4569549-4569568 MS.gene85230:CDS 40.0%
!! TGCTGAAATGGTGTTGCTTT+AGG - chr2.4:4569276-4569295 MS.gene85230:intron 40.0%
!! TGTGAGCAAGATAATGGGTT+TGG + chr2.4:4569065-4569084 None:intergenic 40.0%
!! TGTTTCATTGGCTTTATCCG+GGG - chr2.4:4569550-4569569 MS.gene85230:CDS 40.0%
AAATGCTCCAGGTTCTACTC+GGG - chr2.4:4570240-4570259 MS.gene85230:intron 45.0%
AACAGCTAACCAGTTTGGAG+TGG + chr2.4:4570819-4570838 None:intergenic 45.0%
ACGATTGAGGAACGCAAACA+TGG + chr2.4:4569162-4569181 None:intergenic 45.0%
ACGGATTCATGCATGTGCTT+TGG - chr2.4:4571625-4571644 MS.gene85230:CDS 45.0%
AGCTATCAGGGATGCTGAAA+AGG - chr2.4:4570483-4570502 MS.gene85230:intron 45.0%
ATCTTGCTCACAAACAGCGA+TGG - chr2.4:4569073-4569092 MS.gene85230:intron 45.0%
ATTAATCGGGGATCAAGCTG+CGG - chr2.4:4570027-4570046 MS.gene85230:intron 45.0%
ATTGAATCAACTGGAGCTGC+AGG - chr2.4:4571099-4571118 MS.gene85230:CDS 45.0%
ATTTCTGCTGTGATTCCTGG+TGG - chr2.4:4571725-4571744 MS.gene85230:CDS 45.0%
GAAGAACGAGAGCTTCAACT+AGG + chr2.4:4569123-4569142 None:intergenic 45.0%
GCCAATGAAACACAATCCAC+AGG + chr2.4:4569542-4569561 None:intergenic 45.0%
GCTGCTTGTCCAAAAACTGA+AGG + chr2.4:4571426-4571445 None:intergenic 45.0%
GGATCTCCACATACTTTGCT+TGG + chr2.4:4570798-4570817 None:intergenic 45.0%
TAGGATACTTACAGCTGCAG+AGG + chr2.4:4570927-4570946 None:intergenic 45.0%
TCAAATAGCCGCTATCCAAC+TGG - chr2.4:4570839-4570858 MS.gene85230:intron 45.0%
TCAACTAGGTGTGTAAGACC+TGG + chr2.4:4569109-4569128 None:intergenic 45.0%
TGGATTTCTGCTGTGATTCC+TGG - chr2.4:4571722-4571741 MS.gene85230:CDS 45.0%
TGTTACGATTCGAGAAACCG+TGG - chr2.4:4569322-4569341 MS.gene85230:intron 45.0%
TTGCTCCCTTCTCATTCTGA+CGG - chr2.4:4571606-4571625 MS.gene85230:CDS 45.0%
TTGGCCAAGAAATGACCAGT+TGG + chr2.4:4570857-4570876 None:intergenic 45.0%
! ATCATCGTTGGTGGAAGGAA+CGG + chr2.4:4569008-4569027 None:intergenic 45.0%
! TTCGTGGTCAAAGCCTGTTT+TGG - chr2.4:4569580-4569599 MS.gene85230:CDS 45.0%
!! ATCTGGTATTCTAGGGCGTT+TGG - chr2.4:4570669-4570688 MS.gene85230:intron 45.0%
!! GCTAGGGAGGCATTATTATG+CGG - chr2.4:4570276-4570295 MS.gene85230:intron 45.0%
!! TCAGTTTTTGGACAAGCAGC+AGG - chr2.4:4571426-4571445 MS.gene85230:intron 45.0%
!! TGCATGTGCTTTGGAGAAAC+AGG - chr2.4:4571634-4571653 MS.gene85230:CDS 45.0%
!!! GCCACTGCTTTTGAGATTTC+AGG - chr2.4:4569371-4569390 MS.gene85230:intron 45.0%
AATGCTCCAGGTTCTACTCG+GGG - chr2.4:4570241-4570260 MS.gene85230:intron 50.0%
AGCGATGGAGTTGAATCACC+AGG - chr2.4:4569088-4569107 MS.gene85230:intron 50.0%
ATAGGAGTAGGAGGATGAGG+AGG - chr2.4:4571169-4571188 MS.gene85230:intron 50.0%
CATTTCTTGGCCAATCCGCA+AGG - chr2.4:4570863-4570882 MS.gene85230:intron 50.0%
GAAACAGGTTGCAGTGACTC+CGG - chr2.4:4571649-4571668 MS.gene85230:CDS 50.0%
GCTGCACCCTGAACAAAACA+TGG + chr2.4:4571042-4571061 None:intergenic 50.0%
GCTGTGCAAACTGAAGACCT+AGG + chr2.4:4570892-4570911 None:intergenic 50.0%
GGTCTTCAGTTTGCACAGCT+TGG - chr2.4:4570893-4570912 MS.gene85230:intron 50.0%
TAGGAGTAGGAGGATGAGGA+GGG - chr2.4:4571170-4571189 MS.gene85230:intron 50.0%
TGAAGATTGGAAACGAGGCC+CGG + chr2.4:4568929-4568948 None:intergenic 50.0%
TGTGGAGATCCACTCCAAAC+TGG - chr2.4:4570807-4570826 MS.gene85230:intron 50.0%
! GGGTTATCATCGTTGGTGGA+AGG + chr2.4:4569013-4569032 None:intergenic 50.0%
!! TTACAGCTGCAGAGGCATCT+CGG + chr2.4:4570919-4570938 None:intergenic 50.0%
AACAACCCCCGAGTAGAACC+TGG + chr2.4:4570250-4570269 None:intergenic 55.0%
AAGATTGGAAACGAGGCCCG+GGG + chr2.4:4568927-4568946 None:intergenic 55.0%
AGGAGTAGGAGGATGAGGAG+GGG - chr2.4:4571171-4571190 MS.gene85230:intron 55.0%
ATGCTCCAGGTTCTACTCGG+GGG - chr2.4:4570242-4570261 MS.gene85230:intron 55.0%
GAAGATTGGAAACGAGGCCC+GGG + chr2.4:4568928-4568947 None:intergenic 55.0%
TGAAGACCTAGGCCACCTTG+CGG + chr2.4:4570881-4570900 None:intergenic 55.0%
TGACCACGAAAACAACGCCC+CGG + chr2.4:4569570-4569589 None:intergenic 55.0%
TTCTACTCGGGGGTTGTTGC+TGG - chr2.4:4570252-4570271 MS.gene85230:intron 55.0%
TTCTTGGCCAATCCGCAAGG+TGG - chr2.4:4570866-4570885 MS.gene85230:intron 55.0%
TTGTTCAGGGTGCAGCTCGT+CGG - chr2.4:4571046-4571065 MS.gene85230:intron 55.0%
!!! TATCCGGGGCGTTGTTTTCG+TGG - chr2.4:4569564-4569583 MS.gene85230:CDS 55.0%
ACCTAGGCCACCTTGCGGAT+TGG + chr2.4:4570876-4570895 None:intergenic 60.0%
AGATTGGAAACGAGGCCCGG+GGG + chr2.4:4568926-4568945 None:intergenic 60.0%
GGAGTAGGAGGATGAGGAGG+GGG - chr2.4:4571172-4571191 MS.gene85230:intron 60.0%
GGGGGTTGTTGCTGGAGCTA+GGG - chr2.4:4570260-4570279 MS.gene85230:intron 60.0%
GGTTGTTGCTGGAGCTAGGG+AGG - chr2.4:4570263-4570282 MS.gene85230:intron 60.0%
CGGGGGTTGTTGCTGGAGCT+AGG - chr2.4:4570259-4570278 MS.gene85230:intron 65.0%
GCCAATCCGCAAGGTGGCCT+AGG - chr2.4:4570872-4570891 MS.gene85230:intron 65.0%
TCAGGGTGCAGCTCGTCGGT+TGG - chr2.4:4571050-4571069 MS.gene85230:intron 65.0%


Chromosome Type Strat End Strand Name
chr2.4 gene 4568917 4571754 4568917 ID=MS.gene85230
chr2.4 mRNA 4568917 4571754 4568917 ID=MS.gene85230.t1;Parent=MS.gene85230
chr2.4 exon 4571492 4571754 4571492 ID=MS.gene85230.t1.exon1;Parent=MS.gene85230.t1
chr2.4 CDS 4571492 4571754 4571492 ID=cds.MS.gene85230.t1;Parent=MS.gene85230.t1
chr2.4 exon 4571279 4571373 4571279 ID=MS.gene85230.t1.exon2;Parent=MS.gene85230.t1
chr2.4 CDS 4571279 4571373 4571279 ID=cds.MS.gene85230.t1;Parent=MS.gene85230.t1
chr2.4 exon 4571070 4571137 4571070 ID=MS.gene85230.t1.exon3;Parent=MS.gene85230.t1
chr2.4 CDS 4571070 4571137 4571070 ID=cds.MS.gene85230.t1;Parent=MS.gene85230.t1
chr2.4 exon 4570613 4570656 4570613 ID=MS.gene85230.t1.exon4;Parent=MS.gene85230.t1
chr2.4 CDS 4570613 4570656 4570613 ID=cds.MS.gene85230.t1;Parent=MS.gene85230.t1
chr2.4 exon 4570343 4570420 4570343 ID=MS.gene85230.t1.exon5;Parent=MS.gene85230.t1
chr2.4 CDS 4570343 4570420 4570343 ID=cds.MS.gene85230.t1;Parent=MS.gene85230.t1
chr2.4 exon 4570104 4570257 4570104 ID=MS.gene85230.t1.exon6;Parent=MS.gene85230.t1
chr2.4 CDS 4570104 4570257 4570104 ID=cds.MS.gene85230.t1;Parent=MS.gene85230.t1
chr2.4 exon 4569737 4569889 4569737 ID=MS.gene85230.t1.exon7;Parent=MS.gene85230.t1
chr2.4 CDS 4569737 4569889 4569737 ID=cds.MS.gene85230.t1;Parent=MS.gene85230.t1
chr2.4 exon 4569507 4569617 4569507 ID=MS.gene85230.t1.exon8;Parent=MS.gene85230.t1
chr2.4 CDS 4569507 4569617 4569507 ID=cds.MS.gene85230.t1;Parent=MS.gene85230.t1
chr2.4 exon 4569173 4569241 4569173 ID=MS.gene85230.t1.exon9;Parent=MS.gene85230.t1
chr2.4 CDS 4569173 4569241 4569173 ID=cds.MS.gene85230.t1;Parent=MS.gene85230.t1
chr2.4 exon 4568917 4569015 4568917 ID=MS.gene85230.t1.exon10;Parent=MS.gene85230.t1
chr2.4 CDS 4568917 4569015 4568917 ID=cds.MS.gene85230.t1;Parent=MS.gene85230.t1
Gene Sequence

>MS.gene85230

ATGATGCCCCCGGGCCTCGTTTCCAATCTTCAAGAAGCACTTCTCAACAGAAAAATCACACCTTCACAACAAGATGAATCAAACGACACCGTTCCTTCCACCAACGATGATAACCCTAATCCTAACCCTATCGAATTCGATGAATCCAAACCCATTATCTTGCTCACAAACAGCGATGGAGTTGAATCACCAGGTCTTACACACCTAGTTGAAGCTCTCGTTCTTCAAAACCTTTACAATGTCCATGTTTGCGTTCCTCAATCGTGCGTTCTTTTTTTAATCTGTTAAGAAATGAAGTAAATTTTTGATTTTTTTGTTGGTATTGAATAATGTTGAATGTGTTTGTAATTTTTGTTAGTTGCTGAAATGGTGTTGCTTTAGGGATAAATCTGTTTCTGGTCATTCTGTTACGATTCGAGAAACCGTGGAAGCTTGTTCTACTCAAGTTAATGGTGCCACTGCTTTTGAGATTTCAGGTATTAACTTCATTCAATTCTGTTTCTAAATCTGAAATTTTTGTTTTTTTGGGTTGTGCTTATGAATTTTTTGATATTAATAATGTAATAAGTATATGAATTTGTTAATGTTTTATAATTTAGTTATTTATAATAATTCAGGGACTCCTGTGGATTGTGTTTCATTGGCTTTATCCGGGGCGTTGTTTTCGTGGTCAAAGCCTGTTTTGGTTAGCATTTATTATTGTTTTATAGCTTACACCCGTAAATTAATTGTGTGTTTGGTTCTGCTGTGACAAAGATTGATTTTGATTTGAATGAATTGATTCTGTAAAATTTTAAAATTGATTCTGGCTAAAAATGAGTTGAAGGTAAAATGATTTATTATGTTTGGATACATTTGTGTGAAAGCAAGTCGAACAATAATTTTGAGTCTAAAAATCTGTTCTAGAATAAAAAACTCCAAATTCTGGCTTCTAGTTAGAATGAATTTTGAAAGCAAAATCAATTATACTCAAGAGCAATCAAACAAGTCAAAATCAATTCTATACCTCTGGAATCAATTTTGGCTACCCCAGAAGTTAAACCAAACATACACTAATAGTGCTGCGATATAGTAATCATGATGTGATTTGAATTTCAGGTTATTAGTGGGATTAATCGGGGATCAAGCTGCGGTCATCACATGTAAGTTGCTAACTTATGGTTAGGGAGAAGTTATATAAATCTGAGTTCTGAAATTGGACTTCAACACATTTTCCATTTTCCGTCTTGTAATTTGATTTTGTTTCAAGATTTGTATTTATTGTCCAGAATTTGCTGCCTTGTTATAGAGATGTGTAGAGATGTGTGTTTATGCCATTCTTTAAAATGCTCCAGGTTCTACTCGGGGGTTGTTGCTGGAGCTAGGGAGGCATTATTATGCGGCGTACCATCATTATCAATATCATTAAACTGGTAAGTTGGTGATTCTATACATCTGGTTTTAACTATGCTTTCATGCTCTAGAGATTATGAATTGGTATCGTATATCATGTTACAGGAAGAAGGATGAAAGTCAAGAAACTGATTTCAAGGATGCAGTTGAAGTCTGTTTACCGTTGATAAATGCAGCTATCAGGGATGCTGAAAAGGAAACATTTCCCAAAAATTGCTTTCTGAATATTGAAATTCCAAGATCACCTTTGAGCAACAAGGTGTGTAAATTTTGTTATATAATAATTTGATTTTTTTTTTTTCCGGCATGTTTTACTGCATTGTGCAAAATCTGGTATATTTCTTTCATGTATTGTACAAAATCTGGTATTCTAGGGCGTTTGGCTAATTGATTCTTATGATCAAATTATTTCATAGTTTTTTTTCTTCTCGCATGCTGTTTATACTGATGCAGTGGTATCCTTTTATTGACAGGGCTTTAAATTGACCAAGCAAAGTATGTGGAGATCCACTCCAAACTGGTTAGCTGTTTCAAATAGCCGCTATCCAACTGGTCATTTCTTGGCCAATCCGCAAGGTGGCCTAGGTCTTCAGTTTGCACAGCTTGGCCGAGATGCCTCTGCAGCTGTAAGTATCCTACACTACATACAAACATGTGTTATGTTGATATTTTCTGTCTATTCTCAGGAGTGATTTTAATGTGTTTAATCTATTGATGCTGATACTTGAATCCATGTTTTGTTCAGGGTGCAGCTCGTCGGTTGGCTACGCAGAAAAAGAATTTGGAGATTATTGAATCAACTGGAGCTGCAGGAAAACCTGATACCAATAAAGTGAAGAAATATTTCAGACTAGAGGTAAATAGGAGTAGGAGGATGAGGAGGGGGATCTTGATTAGAATAGAATTTTCTTTGTACAAATCTTGTTAATGTTGAAGTTAGTCTGTTTTCAATGCTGAGTACAGAAGACAACAATTTGTGGAGAATAGATTATCATAGAAGTTATCTTCATACTTGAGAAGCTTTATCTAAGTTTTAACCATGAGTTATATTTGTATTTCAAATAAGTAACATCAAATTATTTGTACAAGTGAACTTAGCTATAATCTACTTGTACCTTCAGTTTTTGGACAAGCAGCAGGAAGAAATAGATGACGATGATCTGGATTACAGAGCACTTGAAAGTGGATATGTAAGTTTGAGCAATAAATTATTTTTTCAATTGCTATTAGTTATCTTTAACTATATTGTATGTTCTTGATGTTAATATCTTTATGGGCTGTCATACATCTACCAATTTTGCTCCCTTCTCATTCTGACGGATTCATGCATGTGCTTTGGAGAAACAGGTTGCAGTGACTCCGGTATCTCTTTCTCCTCATATTGAGAGCGATATTCAAACAACAATTTCTGATTGGATTTCTGCTGTGATTCCTGGTGGACAGTAA
Protein sequence

>MS.gene85230.t1

MMPPGLVSNLQEALLNRKITPSQQDESNDTVPSTNDDNPNPNPIEFDESKPIILLTNSDGVESPGLTHLVEALVLQNLYNVHVCVPQSDKSVSGHSVTIRETVEACSTQVNGATAFEISGTPVDCVSLALSGALFSWSKPVLVISGINRGSSCGHHMFYSGVVAGAREALLCGVPSLSISLNWKKDESQETDFKDAVEVCLPLINAAIRDAEKETFPKNCFLNIEIPRSPLSNKGFKLTKQSMWRSTPNWLAVSNSRYPTGHFLANPQGGLGLQFAQLGRDASAAGAARRLATQKKNLEIIESTGAAGKPDTNKVKKYFRLEFLDKQQEEIDDDDLDYRALESGYVAVTPVSLSPHIESDIQTTISDWISAVIPGGQ