AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene051956

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene051956.t1 MTR_2g099720 97.347 377 10 0 1 377 1 377 0.0 760
MS.gene051956.t1 MTR_5g006290 36.615 325 164 7 53 372 11 298 1.04e-67 216
MS.gene051956.t1 MTR_1g104500 35.241 332 174 6 45 372 3 297 6.35e-66 211
MS.gene051956.t1 MTR_5g006290 47.059 204 106 2 53 255 11 213 1.40e-61 199
MS.gene051956.t1 MTR_5g006300 45.320 203 96 5 47 241 3 198 5.27e-51 172
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene051956.t1 AT1G72880 61.942 381 137 6 2 377 8 385 1.15e-164 466
MS.gene051956.t1 AT1G72880 61.942 381 137 6 2 377 8 385 1.15e-164 466
MS.gene051956.t1 AT4G14930 35.385 325 176 6 50 372 13 305 2.36e-62 202
MS.gene051956.t1 AT4G14930 36.422 313 164 7 50 359 13 293 1.74e-60 197

Find 93 sgRNAs with CRISPR-Local

Find 178 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTAGGGATAAATCTGTTTC+TGG 0.178353 2.2:-3294672 MS.gene051956:intron
GAAGTATTTCTTCACTTTAT+TGG 0.213458 2.2:+3292861 None:intergenic
TGTGTTTCATTGGCTTTATC+TGG 0.234130 2.2:-3294424 MS.gene051956:CDS
GCTTGTTCTGCTCAAGTTAA+TGG 0.246805 2.2:-3294618 MS.gene051956:CDS
TGGATTTCTGCTGTGATTCC+TGG 0.252539 2.2:-3292209 MS.gene051956:CDS
AATTCGATAGGGTTAGGATT+AGG 0.265411 2.2:+3294937 None:intergenic
CGTTGATAAATGCAGCTATC+AGG 0.280646 2.2:-3293529 MS.gene051956:CDS
TTCTTCACTTTATTGGTATC+AGG 0.291732 2.2:+3292868 None:intergenic
ATTCGATAGGGTTAGGATTA+GGG 0.300748 2.2:+3294938 None:intergenic
CTATCCAACTGGTCATTTCT+TGG 0.301621 2.2:-3293144 MS.gene051956:CDS
AGGTTATTAGTGGGATTAAT+CGG 0.308990 2.2:-3293984 MS.gene051956:CDS
TCCTGCGGATTGTGTTTCAT+TGG 0.339731 2.2:-3294434 MS.gene051956:CDS
CTTGTTGCTCAAAGGTGATC+TGG 0.343359 2.2:+3293454 None:intergenic
CTGTTTGTGAGAAGGATAAT+TGG 0.348538 2.2:+3294901 None:intergenic
TTTGAAACAGCTAACCAGTT+TGG 0.357202 2.2:+3293173 None:intergenic
TTGGAGATTATTGAATCAAC+TGG 0.357915 2.2:-3292904 MS.gene051956:CDS
TCATCGAATTCGATAGGGTT+AGG 0.387109 2.2:+3294931 None:intergenic
CATTATCAGTATCATTAAAC+TGG 0.392336 2.2:-3293688 MS.gene051956:intron
TAATAAATGCTAACCAAAAC+AGG 0.394978 2.2:+3294379 None:intergenic
TTCAAACGACAACTTCTGAT+TGG 0.397461 2.2:-3292229 MS.gene051956:CDS
GAGAAGTGCTTCTTGAAGAT+TGG 0.399004 2.2:+3295029 None:intergenic
GGTTATTAGTGGGATTAATC+GGG 0.422540 2.2:-3293983 MS.gene051956:CDS
TTCGTGGTCAAAGCCTGTTT+TGG 0.423001 2.2:-3294392 MS.gene051956:intron
TGAAGATTGGAAACGAGGCC+CGG 0.439122 2.2:+3295042 None:intergenic
AAGTCAAGAAACTGATTTCA+AGG 0.442158 2.2:-3293573 MS.gene051956:CDS
ATCATCGTTGGTTGAAGAAA+CGG 0.442912 2.2:+3294963 None:intergenic
TTTGAATTTCAGGTTATTAG+TGG 0.444025 2.2:-3293994 MS.gene051956:intron
AGAAATAGATGACGATGATC+TGG 0.446591 2.2:-3292483 MS.gene051956:CDS
GAAGATTGGAAACGAGGCCC+GGG 0.453439 2.2:+3295043 None:intergenic
TTGTTGCTCAAAGGTGATCT+GGG 0.455578 2.2:+3293455 None:intergenic
GGTCTTCAGTTTGCACAGCT+TGG 0.463528 2.2:-3293101 MS.gene051956:CDS
GCCAATCCACAAGGTGGCCT+AGG 0.464602 2.2:-3293122 MS.gene051956:CDS
ATTGAATCAACTGGAGCTGC+AGG 0.468057 2.2:-3292895 MS.gene051956:CDS
AGCTATCAGGGATGCTGAAA+AGG 0.478095 2.2:-3293516 MS.gene051956:CDS
TGTGGAGATCCACTCCAAAC+TGG 0.479831 2.2:-3293187 MS.gene051956:CDS
TCAAATAGCCGCTATCCAAC+TGG 0.485041 2.2:-3293155 MS.gene051956:CDS
GGATCTCCACATACTTTGCT+TGG 0.489007 2.2:+3293199 None:intergenic
AGGATTAGGGTTATCATCGT+TGG 0.489597 2.2:+3294951 None:intergenic
ACCTAGGCCACCTTGTGGAT+TGG 0.490198 2.2:+3293121 None:intergenic
TCGTTTCAAGATGATGCCCC+CGG 0.496104 2.2:-3295061 None:intergenic
AAAATGCTCCAGGTTCTACT+CGG 0.500819 2.2:-3293756 MS.gene051956:intron
TTAGATTCATCGAATTCGAT+AGG 0.502616 2.2:+3294925 None:intergenic
TTGAATTTCAGGTTATTAGT+GGG 0.502752 2.2:-3293993 MS.gene051956:intron
CGCAAACATGGACATTGTAA+AGG 0.507879 2.2:+3294821 None:intergenic
TATATCATGTTATAGGAAGA+AGG 0.515481 2.2:-3293600 MS.gene051956:intron
AAATGCTCCAGGTTCTACTC+GGG 0.515947 2.2:-3293755 MS.gene051956:intron
TGAATATCGCTCTCAATATG+AGG 0.517882 2.2:+3292248 None:intergenic
TTTGATTCATCTTGTTGTGA+AGG 0.524748 2.2:+3294991 None:intergenic
TCAGGGTGCAGCTCGTCGGT+TGG 0.528374 2.2:-3292944 MS.gene051956:intron
TTGTTCAGGGTGCAGCTCGT+CGG 0.533664 2.2:-3292948 MS.gene051956:intron
TTACAGACCTTGTTGCTCAA+AGG 0.534339 2.2:+3293446 None:intergenic
GTTGATAAATGCAGCTATCA+GGG 0.535579 2.2:-3293528 MS.gene051956:CDS
TTGAGCAGAACAAGCTTCCA+CGG 0.545035 2.2:+3294626 None:intergenic
CTCCATCGCTGTTTGTGAGA+AGG 0.546172 2.2:+3294893 None:intergenic
GAAGAACGAGAGCTTCAACT+AGG 0.548339 2.2:+3294848 None:intergenic
ATATGAGGAGAAAGAGATAC+CGG 0.550193 2.2:+3292263 None:intergenic
TTTAATGATACTGATAATGA+TGG 0.552788 2.2:+3293692 None:intergenic
TGTTTAATTTACTGTCCACC+AGG 0.555593 2.2:+3292191 None:intergenic
TAGATTCATCGAATTCGATA+GGG 0.558614 2.2:+3294926 None:intergenic
GGTTGTTGCTGGAGCTAGGG+AGG 0.559179 2.2:-3293732 MS.gene051956:CDS
AATTGACCAAGCAAAGTATG+TGG 0.559860 2.2:-3293205 MS.gene051956:CDS
GATTACAGAGCACTTGAAAG+TGG 0.567031 2.2:-3292461 MS.gene051956:CDS
ATTTCTGCTGTGATTCCTGG+TGG 0.569344 2.2:-3292206 MS.gene051956:CDS
GCCAATGAAACACAATCCGC+AGG 0.578293 2.2:+3294433 None:intergenic
AAGATTGGAAACGAGGCCCG+GGG 0.581766 2.2:+3295044 None:intergenic
CATTTCTTGGCCAATCCACA+AGG 0.583050 2.2:-3293131 MS.gene051956:CDS
GAAACAGGTTGCAGTGACTC+CGG 0.585349 2.2:-3292282 MS.gene051956:intron
CAGATCACCTTTGAGCAACA+AGG 0.588553 2.2:-3293453 MS.gene051956:intron
ATCCTTCTCACAAACAGCGA+TGG 0.597000 2.2:-3294895 MS.gene051956:CDS
AAGTATACTTACAGCTGCAG+AGG 0.601559 2.2:+3293070 None:intergenic
ACGATTGAGGAACGCAAACA+TGG 0.602918 2.2:+3294809 None:intergenic
TCAACTAGGTGTGTGAGACC+TGG 0.606431 2.2:+3294862 None:intergenic
CTTCTTGAAGATTGGAAACG+AGG 0.607887 2.2:+3295037 None:intergenic
CTGATAGCTGCATTTATCAA+CGG 0.609132 2.2:+3293530 None:intergenic
ACCTGAAATCTCAAAAGCAG+TGG 0.609300 2.2:+3294593 None:intergenic
AACAGCTAACCAGTTTGGAG+TGG 0.612572 2.2:+3293178 None:intergenic
AATGCTCCAGGTTCTACTCG+GGG 0.618010 2.2:-3293754 MS.gene051956:intron
TTGGCCAAGAAATGACCAGT+TGG 0.621148 2.2:+3293140 None:intergenic
GTTATTAGTGGGATTAATCG+GGG 0.623644 2.2:-3293982 MS.gene051956:CDS
GAAGAAATACTTCAGACAAG+AGG 0.628902 2.2:-3292851 MS.gene051956:intron
TTACAGCTGCAGAGGCATCT+CGG 0.632794 2.2:+3293078 None:intergenic
TGTTGCTCAAAGGTGATCTG+GGG 0.633147 2.2:+3293456 None:intergenic
AGCGATGGAGTTGAATCACC+AGG 0.636526 2.2:-3294880 MS.gene051956:CDS
TAATAATTCAGGGACTCCTG+CGG 0.636885 2.2:-3294449 MS.gene051956:intron
TGAAGACCTAGGCCACCTTG+TGG 0.642303 2.2:+3293116 None:intergenic
AGAAATGACCAGTTGGATAG+CGG 0.646911 2.2:+3293147 None:intergenic
ATGCTCCAGGTTCTACTCGG+GGG 0.678166 2.2:-3293753 MS.gene051956:intron
TTCTTGGCCAATCCACAAGG+TGG 0.682503 2.2:-3293128 MS.gene051956:CDS
TGTTACGATTCGAGAAACCG+TGG 0.686133 2.2:-3294643 MS.gene051956:CDS
ATTAATCGGGGATCAAGCTG+CGG 0.696145 2.2:-3293970 MS.gene051956:CDS
GCTAGGGAGGCATTATTATG+CGG 0.712293 2.2:-3293719 MS.gene051956:CDS
GCTGTGCAAACTGAAGACCT+AGG 0.716901 2.2:+3293105 None:intergenic
AGATTGGAAACGAGGCCCGG+GGG 0.763136 2.2:+3295045 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAGTAATTTATAATAATTCA+GGG - chr2.2:3292791-3292810 MS.gene051956:intron 10.0%
!! TTAGTAATTTATAATAATTC+AGG - chr2.2:3292790-3292809 MS.gene051956:intron 10.0%
!!! AATCTGAAATTTTTGTTTTT+TGG - chr2.2:3292706-3292725 MS.gene051956:intron 15.0%
!!! ATCTGAAATTTTTGTTTTTT+GGG - chr2.2:3292707-3292726 MS.gene051956:intron 15.0%
!! ACATATAGAATATTACAAGA+CGG + chr2.2:3293394-3293413 None:intergenic 20.0%
!! TGATTATGTAAATTTGATTC+TGG - chr2.2:3292958-3292977 MS.gene051956:intron 20.0%
!! TTCAAAATCAATTCTTGTTA+CGG + chr2.2:3292933-3292952 None:intergenic 20.0%
!! TTCATATATTGTACAAAATC+TGG - chr2.2:3293909-3293928 MS.gene051956:intron 20.0%
!! TTTAACTATATTGTTCTTGA+TGG - chr2.2:3294861-3294880 MS.gene051956:CDS 20.0%
!!! AATATTCAGAAAGCAATTTT+TGG + chr2.2:3293769-3293788 None:intergenic 20.0%
!!! ATATTCAGAAAGCAATTTTT+GGG + chr2.2:3293768-3293787 None:intergenic 20.0%
!!! TCAAGTTAGAATAATTTTGA+AGG - chr2.2:3293101-3293120 MS.gene051956:CDS 20.0%
!!! TCTGAAATTTTTGTTTTTTG+GGG - chr2.2:3292708-3292727 MS.gene051956:intron 20.0%
!!! TTTAATGATACTGATAATGA+TGG + chr2.2:3293561-3293580 None:intergenic 20.0%
!!! TTTCATTTGAGATTATGAAT+TGG - chr2.2:3293622-3293641 MS.gene051956:intron 20.0%
! AAACACAAATCTCTATAACA+AGG + chr2.2:3293462-3293481 None:intergenic 25.0%
! CATTATCAGTATCATTAAAC+TGG - chr2.2:3293562-3293581 MS.gene051956:CDS 25.0%
! GGTATCATATATCATGTTAT+AGG - chr2.2:3293643-3293662 MS.gene051956:intron 25.0%
! GTCTTGATTAGAATAGAATT+TGG - chr2.2:3294451-3294470 MS.gene051956:intron 25.0%
! TAATAAATGCTAACCAAAAC+AGG + chr2.2:3292874-3292893 None:intergenic 25.0%
! TATATCATGTTATAGGAAGA+AGG - chr2.2:3293650-3293669 MS.gene051956:intron 25.0%
!! ATTTTTGTTAGTTGCTGAAA+TGG - chr2.2:3292549-3292568 MS.gene051956:intron 25.0%
!! GAAGTATTTCTTCACTTTAT+TGG + chr2.2:3294392-3294411 None:intergenic 25.0%
!! GGTAATATCTTTGACTTTAT+GGG - chr2.2:3294882-3294901 MS.gene051956:CDS 25.0%
!! TGGTAATATCTTTGACTTTA+TGG - chr2.2:3294881-3294900 MS.gene051956:CDS 25.0%
!!! TATACCTCTAGAATCAATTT+TGG - chr2.2:3293171-3293190 MS.gene051956:CDS 25.0%
!!! TTGAATTTCAGGTTATTAGT+GGG - chr2.2:3293257-3293276 MS.gene051956:intron 25.0%
!!! TTTGAATTTCAGGTTATTAG+TGG - chr2.2:3293256-3293275 MS.gene051956:intron 25.0%
ACAATGCAGTAAAACATATG+GGG + chr2.2:3293861-3293880 None:intergenic 30.0%
ACATGATGTTGTTGTATGAA+GGG + chr2.2:3294649-3294668 None:intergenic 30.0%
ACATGTAAGTTGCTAACTTA+TGG - chr2.2:3293308-3293327 MS.gene051956:intron 30.0%
AGGTTATTAGTGGGATTAAT+CGG - chr2.2:3293266-3293285 MS.gene051956:intron 30.0%
CAAACACGCAATTAATTTAC+GGG + chr2.2:3292904-3292923 None:intergenic 30.0%
CACAATGCAGTAAAACATAT+GGG + chr2.2:3293862-3293881 None:intergenic 30.0%
CATGATGTGATTTGAATTTC+AGG - chr2.2:3293246-3293265 MS.gene051956:intron 30.0%
CCAAACACGCAATTAATTTA+CGG + chr2.2:3292905-3292924 None:intergenic 30.0%
GAATATTACAAGACGGAAAA+TGG + chr2.2:3293387-3293406 None:intergenic 30.0%
GATACTTTCTGTCTATTCTT+AGG - chr2.2:3294229-3294248 MS.gene051956:intron 30.0%
GTACAGAAGATAACAATTTG+TGG - chr2.2:3294522-3294541 MS.gene051956:intron 30.0%
GTATCATTAAACTGGTAAGT+TGG - chr2.2:3293570-3293589 MS.gene051956:CDS 30.0%
TAAGTTGCTAACTTATGGTT+GGG - chr2.2:3293313-3293332 MS.gene051956:intron 30.0%
TAGATTCATCGAATTCGATA+GGG + chr2.2:3292327-3292346 None:intergenic 30.0%
TATAACAAGGCTGCAAATTA+TGG + chr2.2:3293449-3293468 None:intergenic 30.0%
TGAAGTTCAATTTCAGAACT+CGG + chr2.2:3293355-3293374 None:intergenic 30.0%
TGTACAAAATCTGGTATTCT+AGG - chr2.2:3293918-3293937 MS.gene051956:intron 30.0%
TTAGATTCATCGAATTCGAT+AGG + chr2.2:3292328-3292347 None:intergenic 30.0%
TTCTTCACTTTATTGGTATC+AGG + chr2.2:3294385-3294404 None:intergenic 30.0%
TTGGAGATTATTGAATCAAC+TGG - chr2.2:3294346-3294365 MS.gene051956:intron 30.0%
TTTAGGGATAAATCTGTTTC+TGG - chr2.2:3292578-3292597 MS.gene051956:intron 30.0%
! AAGTCAAGAAACTGATTTCA+AGG - chr2.2:3293677-3293696 MS.gene051956:intron 30.0%
! AATGGTATCCTTTTATTGAC+AGG - chr2.2:3294016-3294035 MS.gene051956:intron 30.0%
! ATGGTATCCTTTTATTGACA+GGG - chr2.2:3294017-3294036 MS.gene051956:intron 30.0%
! TTTGATTCATCTTGTTGTGA+AGG + chr2.2:3292262-3292281 None:intergenic 30.0%
!! TTGAATCCATGTTTTGTTCA+GGG - chr2.2:3294289-3294308 MS.gene051956:intron 30.0%
AAAAAAAGAACGCACGATTG+AGG + chr2.2:3292457-3292476 None:intergenic 35.0%
AAATCAATTCTTGTTACGGC+AGG + chr2.2:3292929-3292948 None:intergenic 35.0%
AAGTTGCTAACTTATGGTTG+GGG - chr2.2:3293314-3293333 MS.gene051956:intron 35.0%
AATTCGATAGGGTTAGGATT+AGG + chr2.2:3292316-3292335 None:intergenic 35.0%
AATTGACCAAGCAAAGTATG+TGG - chr2.2:3294045-3294064 MS.gene051956:intron 35.0%
ACTTCAGACAAGAGGTAAAT+AGG - chr2.2:3294407-3294426 MS.gene051956:CDS 35.0%
AGAAATAGATGACGATGATC+TGG - chr2.2:3294767-3294786 MS.gene051956:intron 35.0%
ATATGAGGAGAAAGAGATAC+CGG + chr2.2:3294990-3295009 None:intergenic 35.0%
ATTCGATAGGGTTAGGATTA+GGG + chr2.2:3292315-3292334 None:intergenic 35.0%
CAATGCAGTAAAACATATGG+GGG + chr2.2:3293860-3293879 None:intergenic 35.0%
CACATGATGTTGTTGTATGA+AGG + chr2.2:3294650-3294669 None:intergenic 35.0%
CCGTAAATTAATTGCGTGTT+TGG - chr2.2:3292902-3292921 MS.gene051956:CDS 35.0%
CTGATAGCTGCATTTATCAA+CGG + chr2.2:3293723-3293742 None:intergenic 35.0%
CTGTTTGTGAGAAGGATAAT+TGG + chr2.2:3292352-3292371 None:intergenic 35.0%
GAAGAAATACTTCAGACAAG+AGG - chr2.2:3294399-3294418 MS.gene051956:CDS 35.0%
GCAAAGAACAGTATAACTCA+TGG + chr2.2:3294605-3294624 None:intergenic 35.0%
GCACAATGCAGTAAAACATA+TGG + chr2.2:3293863-3293882 None:intergenic 35.0%
GGTTATTAGTGGGATTAATC+GGG - chr2.2:3293267-3293286 MS.gene051956:intron 35.0%
GTAAGTTGCTAACTTATGGT+TGG - chr2.2:3293312-3293331 MS.gene051956:intron 35.0%
GTTATTAGTGGGATTAATCG+GGG - chr2.2:3293268-3293287 MS.gene051956:intron 35.0%
TACTGTTTACACTGATGCAA+TGG - chr2.2:3293998-3294017 MS.gene051956:intron 35.0%
TGAATATCGCTCTCAATATG+AGG + chr2.2:3295005-3295024 None:intergenic 35.0%
TTCAAACGACAACTTCTGAT+TGG - chr2.2:3295021-3295040 MS.gene051956:CDS 35.0%
! ATCATCGTTGGTTGAAGAAA+CGG + chr2.2:3292290-3292309 None:intergenic 35.0%
! CTACTTGTACCTTCAGTTTT+TGG - chr2.2:3294731-3294750 MS.gene051956:intron 35.0%
! GTAGCCAAAATTGATTCTAG+AGG + chr2.2:3293178-3293197 None:intergenic 35.0%
! GTTGATAAATGCAGCTATCA+GGG - chr2.2:3293722-3293741 MS.gene051956:CDS 35.0%
! TGTATGTGTGGTTTAACTTC+TGG + chr2.2:3293202-3293221 None:intergenic 35.0%
! TTTGAAACAGCTAACCAGTT+TGG + chr2.2:3294080-3294099 None:intergenic 35.0%
! TTTGAAGCCCTGTCAATAAA+AGG + chr2.2:3294027-3294046 None:intergenic 35.0%
!! AGTTGGTGATTCTATACATC+TGG - chr2.2:3293587-3293606 MS.gene051956:CDS 35.0%
!! ATCTGGTATTCTAGGACATT+TGG - chr2.2:3293926-3293945 MS.gene051956:intron 35.0%
!! CTTGAATCCATGTTTTGTTC+AGG - chr2.2:3294288-3294307 MS.gene051956:intron 35.0%
!! TGTGTTTCATTGGCTTTATC+TGG - chr2.2:3292826-3292845 MS.gene051956:intron 35.0%
AAAATGCTCCAGGTTCTACT+CGG - chr2.2:3293494-3293513 MS.gene051956:CDS 40.0%
AAGAGGTAAATAGGAGGATG+AGG - chr2.2:3294416-3294435 MS.gene051956:CDS 40.0%
AAGTATACTTACAGCTGCAG+AGG + chr2.2:3294183-3294202 None:intergenic 40.0%
ACCTGAAATCTCAAAAGCAG+TGG + chr2.2:3292660-3292679 None:intergenic 40.0%
AGAAATGACCAGTTGGATAG+CGG + chr2.2:3294106-3294125 None:intergenic 40.0%
AGGATTAGGGTTATCATCGT+TGG + chr2.2:3292302-3292321 None:intergenic 40.0%
ATGAATCCGTCAGAATGAGA+AGG + chr2.2:3294934-3294953 None:intergenic 40.0%
CAGCACTATTAGTGTATGTG+TGG + chr2.2:3293214-3293233 None:intergenic 40.0%
CGCAAACATGGACATTGTAA+AGG + chr2.2:3292432-3292451 None:intergenic 40.0%
CTATCCAACTGGTCATTTCT+TGG - chr2.2:3294106-3294125 MS.gene051956:intron 40.0%
CTTCTTGAAGATTGGAAACG+AGG + chr2.2:3292216-3292235 None:intergenic 40.0%
GAATGAGAAGGGAGCAAAAT+TGG + chr2.2:3294922-3294941 None:intergenic 40.0%
GCTTGTTCTGCTCAAGTTAA+TGG - chr2.2:3292632-3292651 MS.gene051956:intron 40.0%
GGCTACGCAGAAAAAGAATT+TGG - chr2.2:3294327-3294346 MS.gene051956:intron 40.0%
TAAATAGGAGGATGAGGATG+AGG - chr2.2:3294422-3294441 MS.gene051956:CDS 40.0%
TAATAATTCAGGGACTCCTG+CGG - chr2.2:3292801-3292820 MS.gene051956:intron 40.0%
TCAGACAAGAGGTAAATAGG+AGG - chr2.2:3294410-3294429 MS.gene051956:CDS 40.0%
TCATCGAATTCGATAGGGTT+AGG + chr2.2:3292322-3292341 None:intergenic 40.0%
TGAATCCGTCAGAATGAGAA+GGG + chr2.2:3294933-3294952 None:intergenic 40.0%
TTACAGACCTTGTTGCTCAA+AGG + chr2.2:3293807-3293826 None:intergenic 40.0%
TTGTTGCTCAAAGGTGATCT+GGG + chr2.2:3293798-3293817 None:intergenic 40.0%
! CAACTTGCTTTCACACATGA+AGG + chr2.2:3293024-3293043 None:intergenic 40.0%
! CGTTGATAAATGCAGCTATC+AGG - chr2.2:3293721-3293740 MS.gene051956:CDS 40.0%
! GATTACAGAGCACTTGAAAG+TGG - chr2.2:3294789-3294808 MS.gene051956:intron 40.0%
!! GAGAAGTGCTTCTTGAAGAT+TGG + chr2.2:3292224-3292243 None:intergenic 40.0%
!! GCTGAAATGGTGTTGCTTTA+GGG - chr2.2:3292562-3292581 MS.gene051956:intron 40.0%
!! TGCTGAAATGGTGTTGCTTT+AGG - chr2.2:3292561-3292580 MS.gene051956:intron 40.0%
!!! ACCTGGAGCATTTTAAAGCA+TGG + chr2.2:3293488-3293507 None:intergenic 40.0%
!!! TATCTGGAGCTTTGTTTTCG+TGG - chr2.2:3292842-3292861 MS.gene051956:intron 40.0%
AAATGCTCCAGGTTCTACTC+GGG - chr2.2:3293495-3293514 MS.gene051956:CDS 45.0%
AACAGCTAACCAGTTTGGAG+TGG + chr2.2:3294075-3294094 None:intergenic 45.0%
ACGATTGAGGAACGCAAACA+TGG + chr2.2:3292444-3292463 None:intergenic 45.0%
ACGGATTCATGCATGTGCTT+TGG - chr2.2:3294944-3294963 MS.gene051956:CDS 45.0%
AGCTATCAGGGATGCTGAAA+AGG - chr2.2:3293734-3293753 MS.gene051956:CDS 45.0%
ATCCTTCTCACAAACAGCGA+TGG - chr2.2:3292355-3292374 MS.gene051956:intron 45.0%
ATTAATCGGGGATCAAGCTG+CGG - chr2.2:3293280-3293299 MS.gene051956:intron 45.0%
ATTGAATCAACTGGAGCTGC+AGG - chr2.2:3294355-3294374 MS.gene051956:intron 45.0%
ATTTCTGCTGTGATTCCTGG+TGG - chr2.2:3295044-3295063 MS.gene051956:CDS 45.0%
CAGATCACCTTTGAGCAACA+AGG - chr2.2:3293797-3293816 MS.gene051956:intron 45.0%
CATTTCTTGGCCAATCCACA+AGG - chr2.2:3294119-3294138 MS.gene051956:intron 45.0%
CTTGTTGCTCAAAGGTGATC+TGG + chr2.2:3293799-3293818 None:intergenic 45.0%
GAAGAACGAGAGCTTCAACT+AGG + chr2.2:3292405-3292424 None:intergenic 45.0%
GCTGCTTGTCCAAAAACTGA+AGG + chr2.2:3294743-3294762 None:intergenic 45.0%
GGATCTCCACATACTTTGCT+TGG + chr2.2:3294054-3294073 None:intergenic 45.0%
TCAAATAGCCGCTATCCAAC+TGG - chr2.2:3294095-3294114 MS.gene051956:intron 45.0%
TGGATTTCTGCTGTGATTCC+TGG - chr2.2:3295041-3295060 MS.gene051956:CDS 45.0%
TGTTACGATTCGAGAAACCG+TGG - chr2.2:3292607-3292626 MS.gene051956:intron 45.0%
TGTTGCTCAAAGGTGATCTG+GGG + chr2.2:3293797-3293816 None:intergenic 45.0%
TTGAGCAGAACAAGCTTCCA+CGG + chr2.2:3292627-3292646 None:intergenic 45.0%
TTGCTCCCTTCTCATTCTGA+CGG - chr2.2:3294925-3294944 MS.gene051956:CDS 45.0%
TTGGCCAAGAAATGACCAGT+TGG + chr2.2:3294113-3294132 None:intergenic 45.0%
! TCCTGCGGATTGTGTTTCAT+TGG - chr2.2:3292816-3292835 MS.gene051956:intron 45.0%
! TTCGTGGTCAAAGCCTGTTT+TGG - chr2.2:3292858-3292877 MS.gene051956:CDS 45.0%
!! GCCATGCTTTAAAATGCTCC+AGG - chr2.2:3293484-3293503 MS.gene051956:CDS 45.0%
!! GCTAGGGAGGCATTATTATG+CGG - chr2.2:3293531-3293550 MS.gene051956:CDS 45.0%
!! TCAGTTTTTGGACAAGCAGC+AGG - chr2.2:3294743-3294762 MS.gene051956:intron 45.0%
!! TGCATGTGCTTTGGAGAAAC+AGG - chr2.2:3294953-3294972 MS.gene051956:CDS 45.0%
!!! GCCACTGCTTTTGAGATTTC+AGG - chr2.2:3292656-3292675 MS.gene051956:intron 45.0%
AATGCTCCAGGTTCTACTCG+GGG - chr2.2:3293496-3293515 MS.gene051956:CDS 50.0%
AGCGATGGAGTTGAATCACC+AGG - chr2.2:3292370-3292389 MS.gene051956:intron 50.0%
ATAGGAGGATGAGGATGAGG+AGG - chr2.2:3294425-3294444 MS.gene051956:CDS 50.0%
CTCCATCGCTGTTTGTGAGA+AGG + chr2.2:3292360-3292379 None:intergenic 50.0%
GAAACAGGTTGCAGTGACTC+CGG - chr2.2:3294968-3294987 MS.gene051956:CDS 50.0%
GCCAATGAAACACAATCCGC+AGG + chr2.2:3292820-3292839 None:intergenic 50.0%
GCTGCACCCTGAACAAAACA+TGG + chr2.2:3294298-3294317 None:intergenic 50.0%
GCTGTGCAAACTGAAGACCT+AGG + chr2.2:3294148-3294167 None:intergenic 50.0%
GGTCTTCAGTTTGCACAGCT+TGG - chr2.2:3294149-3294168 MS.gene051956:intron 50.0%
TAGGAGGATGAGGATGAGGA+GGG - chr2.2:3294426-3294445 MS.gene051956:CDS 50.0%
TCAACTAGGTGTGTGAGACC+TGG + chr2.2:3292391-3292410 None:intergenic 50.0%
TGAAGATTGGAAACGAGGCC+CGG + chr2.2:3292211-3292230 None:intergenic 50.0%
TGTGGAGATCCACTCCAAAC+TGG - chr2.2:3294063-3294082 MS.gene051956:intron 50.0%
TTCTTGGCCAATCCACAAGG+TGG - chr2.2:3294122-3294141 MS.gene051956:intron 50.0%
!! TTACAGCTGCAGAGGCATCT+CGG + chr2.2:3294175-3294194 None:intergenic 50.0%
AACAACCCCCGAGTAGAACC+TGG + chr2.2:3293505-3293524 None:intergenic 55.0%
AAGATTGGAAACGAGGCCCG+GGG + chr2.2:3292209-3292228 None:intergenic 55.0%
ACCTAGGCCACCTTGTGGAT+TGG + chr2.2:3294132-3294151 None:intergenic 55.0%
AGGAGGATGAGGATGAGGAG+GGG - chr2.2:3294427-3294446 MS.gene051956:CDS 55.0%
ATGCTCCAGGTTCTACTCGG+GGG - chr2.2:3293497-3293516 MS.gene051956:CDS 55.0%
GAAGATTGGAAACGAGGCCC+GGG + chr2.2:3292210-3292229 None:intergenic 55.0%
TGAAGACCTAGGCCACCTTG+TGG + chr2.2:3294137-3294156 None:intergenic 55.0%
TTCTACTCGGGGGTTGTTGC+TGG - chr2.2:3293507-3293526 MS.gene051956:CDS 55.0%
TTGTTCAGGGTGCAGCTCGT+CGG - chr2.2:3294302-3294321 MS.gene051956:intron 55.0%
AGATTGGAAACGAGGCCCGG+GGG + chr2.2:3292208-3292227 None:intergenic 60.0%
GAGGATGAGGATGAGGAGGG+GGG - chr2.2:3294429-3294448 MS.gene051956:CDS 60.0%
GCCAATCCACAAGGTGGCCT+AGG - chr2.2:3294128-3294147 MS.gene051956:intron 60.0%
GGAGGATGAGGATGAGGAGG+GGG - chr2.2:3294428-3294447 MS.gene051956:CDS 60.0%
GGGGGTTGTTGCTGGAGCTA+GGG - chr2.2:3293515-3293534 MS.gene051956:CDS 60.0%
GGTTGTTGCTGGAGCTAGGG+AGG - chr2.2:3293518-3293537 MS.gene051956:CDS 60.0%
CGGGGGTTGTTGCTGGAGCT+AGG - chr2.2:3293514-3293533 MS.gene051956:CDS 65.0%
TCAGGGTGCAGCTCGTCGGT+TGG - chr2.2:3294306-3294325 MS.gene051956:intron 65.0%


Chromosome Type Strat End Strand Name
chr2.2 gene 3292199 3295073 3292199 ID=MS.gene051956
chr2.2 mRNA 3292199 3295073 3292199 ID=MS.gene051956.t1;Parent=MS.gene051956
chr2.2 exon 3294811 3295073 3294811 ID=MS.gene051956.t1.exon1;Parent=MS.gene051956.t1
chr2.2 CDS 3294811 3295073 3294811 ID=cds.MS.gene051956.t1;Parent=MS.gene051956.t1
chr2.2 exon 3294595 3294689 3294595 ID=MS.gene051956.t1.exon2;Parent=MS.gene051956.t1
chr2.2 CDS 3294595 3294689 3294595 ID=cds.MS.gene051956.t1;Parent=MS.gene051956.t1
chr2.2 exon 3294393 3294460 3294393 ID=MS.gene051956.t1.exon3;Parent=MS.gene051956.t1
chr2.2 CDS 3294393 3294460 3294393 ID=cds.MS.gene051956.t1;Parent=MS.gene051956.t1
chr2.2 exon 3293961 3294004 3293961 ID=MS.gene051956.t1.exon4;Parent=MS.gene051956.t1
chr2.2 CDS 3293961 3294004 3293961 ID=cds.MS.gene051956.t1;Parent=MS.gene051956.t1
chr2.2 exon 3293689 3293766 3293689 ID=MS.gene051956.t1.exon5;Parent=MS.gene051956.t1
chr2.2 CDS 3293689 3293766 3293689 ID=cds.MS.gene051956.t1;Parent=MS.gene051956.t1
chr2.2 exon 3293454 3293607 3293454 ID=MS.gene051956.t1.exon6;Parent=MS.gene051956.t1
chr2.2 CDS 3293454 3293607 3293454 ID=cds.MS.gene051956.t1;Parent=MS.gene051956.t1
chr2.2 exon 3293082 3293234 3293082 ID=MS.gene051956.t1.exon7;Parent=MS.gene051956.t1
chr2.2 CDS 3293082 3293234 3293082 ID=cds.MS.gene051956.t1;Parent=MS.gene051956.t1
chr2.2 exon 3292852 3292962 3292852 ID=MS.gene051956.t1.exon8;Parent=MS.gene051956.t1
chr2.2 CDS 3292852 3292962 3292852 ID=cds.MS.gene051956.t1;Parent=MS.gene051956.t1
chr2.2 exon 3292457 3292525 3292457 ID=MS.gene051956.t1.exon9;Parent=MS.gene051956.t1
chr2.2 CDS 3292457 3292525 3292457 ID=cds.MS.gene051956.t1;Parent=MS.gene051956.t1
chr2.2 exon 3292199 3292297 3292199 ID=MS.gene051956.t1.exon10;Parent=MS.gene051956.t1
chr2.2 CDS 3292199 3292297 3292199 ID=cds.MS.gene051956.t1;Parent=MS.gene051956.t1
Gene Sequence

>MS.gene051956

ATGATGCCCCCGGGCCTCGTTTCCAATCTTCAAGAAGCACTTCTCAACAGAAAAATCACACCTTCACAACAAGATGAATCAAACGACACCGTTTCTTCAACCAACGATGATAACCCTAATCCTAACCCTATCGAATTCGATGAATCTAAACCAATTATCCTTCTCACAAACAGCGATGGAGTTGAATCACCAGGTCTCACACACCTAGTTGAAGCTCTCGTTCTTCAAAACCTTTACAATGTCCATGTTTGCGTTCCTCAATCGTGCGTTCTTTTTTTAATCTGTTAAGAAATGAAGTAAATTTTTGATTTTTTTTTTGTTGTTATTGAATAATGTTGAATGTGTTTGTAATTTTTGTTAGTTGCTGAAATGGTGTTGCTTTAGGGATAAATCTGTTTCTGGTCATTCTGTTACGATTCGAGAAACCGTGGAAGCTTGTTCTGCTCAAGTTAATGGTGCCACTGCTTTTGAGATTTCAGGTATTAACTTCATTCAATTCTGTTTCTAAATCTGAAATTTTTGTTTTTTGGGGTGTGCTTATGAATTTTTTGATATTAATAATGTAATATGAATTTGTTAACGTTTTCTAATTTAGTAATTTATAATAATTCAGGGACTCCTGCGGATTGTGTTTCATTGGCTTTATCTGGAGCTTTGTTTTCGTGGTCAAAGCCTGTTTTGGTTAGCATTTATTATCTTACACCCGTAAATTAATTGCGTGTTTGGTCCTGCCGTAACAAGAATTGATTTTGAATAAATTGATTATGTAAATTTGATTCTGGCTTAAAGTGAGTTGAAAGTAAAATTATTTATGTTTGAATACCTTCATGTGTGAAAGCAAGTTGAACGATAAATTTGAGACTAGAAATCAGTTCTAGAATCAAAAGCTTCAAATTCTAGCTTCAAGTTAGAATAATTTTGAAGGCAGAATCAATTATACTCGAGAGCAATCAAACATGTCAAAATCAATTCTATACCTCTAGAATCAATTTTGGCTACTCCAGAAGTTAAACCACACATACACTAATAGTGCTGTGATATAGTAATCATGATGTGATTTGAATTTCAGGTTATTAGTGGGATTAATCGGGGATCAAGCTGCGGTCATCACATGTAAGTTGCTAACTTATGGTTGGGGAGAAGTTATATAAATCCGAGTTCTGAAATTGAACTTCAACACATTTTCCATTTTCCGTCTTGTAATATTCTATATGTATTGTTTTTGTTTTAAGATTTGTATCTATTGTCCATAATTTGCAGCCTTGTTATAGAGATTTGTGTTTATGCCATGCTTTAAAATGCTCCAGGTTCTACTCGGGGGTTGTTGCTGGAGCTAGGGAGGCATTATTATGCGGCGTACCATCATTATCAGTATCATTAAACTGGTAAGTTGGTGATTCTATACATCTGGTTTTAACTATGCTTTCATTTGAGATTATGAATTGGTATCATATATCATGTTATAGGAAGAAGGATGAAAGTCAAGAAACTGATTTCAAGGATGCAGTTGAAGTCTGTTTACCGTTGATAAATGCAGCTATCAGGGATGCTGAAAAGGAAACATTTCCCAAAAATTGCTTTCTGAATATTGAAATCCCCAGATCACCTTTGAGCAACAAGGTCTGTAAATTTGTTAAATAATAATTTGATTGTTTTTTCCCCCATATGTTTTACTGCATTGTGCAAATGCTCTAGTCTTCTAAGTTTTCTTTCATATATTGTACAAAATCTGGTATTCTAGGACATTTGGCTAATTGATTCTAATGATCAAATTATTTCATAGTTTTTTTTTTCTCGCATACTGTTTACACTGATGCAATGGTATCCTTTTATTGACAGGGCTTCAAATTGACCAAGCAAAGTATGTGGAGATCCACTCCAAACTGGTTAGCTGTTTCAAATAGCCGCTATCCAACTGGTCATTTCTTGGCCAATCCACAAGGTGGCCTAGGTCTTCAGTTTGCACAGCTTGGCCGAGATGCCTCTGCAGCTGTAAGTATACTTCATTACATACAAACATGTGTTATGTTGATACTTTCTGTCTATTCTTAGGTGTGATTTTAATGTGTTTAATCTATTGATGCTGATACTTGAATCCATGTTTTGTTCAGGGTGCAGCTCGTCGGTTGGCTACGCAGAAAAAGAATTTGGAGATTATTGAATCAACTGGAGCTGCAGGAAAACCTGATACCAATAAAGTGAAGAAATACTTCAGACAAGAGGTAAATAGGAGGATGAGGATGAGGAGGGGGGTCTTGATTAGAATAGAATTTGGTTTGTACAAATCTTGTTAATGTTGAAGTTAATCTGTTTTCAATGCTGAGTACAGAAGATAACAATTTGTGGAGAATAGATTATCATAGAAGTTATCTTCATACTTGAGAAGCTTTATCTAAGTTTTAACCATGAGTTATACTGTTCTTTGCATTTTAGTTTATTAATCTGTTCCCTTCATACAACAACATCATGTGTTATAGTTGTATTTCAAATAAGCAACATCAAATTATTTGTACAAGTGAACTTAGCTATAATCTACTTGTACCTTCAGTTTTTGGACAAGCAGCAGGAAGAAATAGATGACGATGATCTGGATTACAGAGCACTTGAAAGTGGATATGTAAGTTTGAGCAATAAATTATTTCTTCAATTGATATTAGTTATCTTTAACTATATTGTTCTTGATGGTAATATCTTTGACTTTATGGGCTGTCATACATTTACCAATTTTGCTCCCTTCTCATTCTGACGGATTCATGCATGTGCTTTGGAGAAACAGGTTGCAGTGACTCCGGTATCTCTTTCTCCTCATATTGAGAGCGATATTCAAACGACAACTTCTGATTGGATTTCTGCTGTGATTCCTGGTGGACAGTAA
Protein sequence

>MS.gene051956.t1

MMPPGLVSNLQEALLNRKITPSQQDESNDTVSSTNDDNPNPNPIEFDESKPIILLTNSDGVESPGLTHLVEALVLQNLYNVHVCVPQSDKSVSGHSVTIRETVEACSAQVNGATAFEISGTPADCVSLALSGALFSWSKPVLVISGINRGSSCGHHMFYSGVVAGAREALLCGVPSLSVSLNWKKDESQETDFKDAVEVCLPLINAAIRDAEKETFPKNCFLNIEIPRSPLSNKGFKLTKQSMWRSTPNWLAVSNSRYPTGHFLANPQGGLGLQFAQLGRDASAAGAARRLATQKKNLEIIESTGAAGKPDTNKVKKYFRQEFLDKQQEEIDDDDLDYRALESGYVAVTPVSLSPHIESDIQTTTSDWISAVIPGGQ