AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene76157

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene76157.t1 MTR_2g099720 97.347 377 10 0 1 377 1 377 0.0 760
MS.gene76157.t1 MTR_5g006290 36.615 325 164 7 53 372 11 298 1.04e-67 216
MS.gene76157.t1 MTR_1g104500 35.241 332 174 6 45 372 3 297 6.35e-66 211
MS.gene76157.t1 MTR_5g006290 47.059 204 106 2 53 255 11 213 1.40e-61 199
MS.gene76157.t1 MTR_5g006300 45.320 203 96 5 47 241 3 198 5.27e-51 172
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene76157.t1 AT1G72880 61.942 381 137 6 2 377 8 385 1.15e-164 466
MS.gene76157.t1 AT1G72880 61.942 381 137 6 2 377 8 385 1.15e-164 466
MS.gene76157.t1 AT4G14930 35.385 325 176 6 50 372 13 305 2.36e-62 202
MS.gene76157.t1 AT4G14930 36.422 313 164 7 50 359 13 293 1.74e-60 197

Find 93 sgRNAs with CRISPR-Local

Find 178 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTAGGGATAAATCTGTTTC+TGG 0.178353 2.3:-3984089 MS.gene76157:intron
GAAGTATTTCTTCACTTTAT+TGG 0.213458 2.3:+3982278 None:intergenic
TGTGTTTCATTGGCTTTATC+TGG 0.234130 2.3:-3983841 MS.gene76157:CDS
GCTTGTTCTGCTCAAGTTAA+TGG 0.246805 2.3:-3984035 MS.gene76157:CDS
TGGATTTCTGCTGTGATTCC+TGG 0.252539 2.3:-3981626 MS.gene76157:CDS
AATTCGATAGGGTTAGGATT+AGG 0.265411 2.3:+3984354 None:intergenic
CGTTGATAAATGCAGCTATC+AGG 0.280646 2.3:-3982946 MS.gene76157:CDS
TTCTTCACTTTATTGGTATC+AGG 0.291732 2.3:+3982285 None:intergenic
ATTCGATAGGGTTAGGATTA+GGG 0.300748 2.3:+3984355 None:intergenic
CTATCCAACTGGTCATTTCT+TGG 0.301621 2.3:-3982561 MS.gene76157:CDS
AGGTTATTAGTGGGATTAAT+CGG 0.308990 2.3:-3983401 MS.gene76157:CDS
TCCTGCGGATTGTGTTTCAT+TGG 0.339731 2.3:-3983851 MS.gene76157:CDS
CTTGTTGCTCAAAGGTGATC+TGG 0.343359 2.3:+3982871 None:intergenic
CTGTTTGTGAGAAGGATAAT+TGG 0.348538 2.3:+3984318 None:intergenic
TTTGAAACAGCTAACCAGTT+TGG 0.357202 2.3:+3982590 None:intergenic
TTGGAGATTATTGAATCAAC+TGG 0.357915 2.3:-3982321 MS.gene76157:CDS
TCATCGAATTCGATAGGGTT+AGG 0.387109 2.3:+3984348 None:intergenic
CATTATCAGTATCATTAAAC+TGG 0.392336 2.3:-3983105 MS.gene76157:intron
TAATAAATGCTAACCAAAAC+AGG 0.394978 2.3:+3983796 None:intergenic
TTCAAACGACAACTTCTGAT+TGG 0.397461 2.3:-3981646 MS.gene76157:CDS
GAGAAGTGCTTCTTGAAGAT+TGG 0.399004 2.3:+3984446 None:intergenic
GGTTATTAGTGGGATTAATC+GGG 0.422540 2.3:-3983400 MS.gene76157:CDS
TTCGTGGTCAAAGCCTGTTT+TGG 0.423001 2.3:-3983809 MS.gene76157:intron
TGAAGATTGGAAACGAGGCC+CGG 0.439122 2.3:+3984459 None:intergenic
AAGTCAAGAAACTGATTTCA+AGG 0.442158 2.3:-3982990 MS.gene76157:CDS
ATCATCGTTGGTTGAAGAAA+CGG 0.442912 2.3:+3984380 None:intergenic
TTTGAATTTCAGGTTATTAG+TGG 0.444025 2.3:-3983411 MS.gene76157:intron
AGAAATAGATGACGATGATC+TGG 0.446591 2.3:-3981900 MS.gene76157:CDS
GAAGATTGGAAACGAGGCCC+GGG 0.453439 2.3:+3984460 None:intergenic
TTGTTGCTCAAAGGTGATCT+GGG 0.455578 2.3:+3982872 None:intergenic
GGTCTTCAGTTTGCACAGCT+TGG 0.463528 2.3:-3982518 MS.gene76157:CDS
GCCAATCCACAAGGTGGCCT+AGG 0.464602 2.3:-3982539 MS.gene76157:CDS
ATTGAATCAACTGGAGCTGC+AGG 0.468057 2.3:-3982312 MS.gene76157:CDS
AGCTATCAGGGATGCTGAAA+AGG 0.478095 2.3:-3982933 MS.gene76157:CDS
TGTGGAGATCCACTCCAAAC+TGG 0.479831 2.3:-3982604 MS.gene76157:CDS
TCAAATAGCCGCTATCCAAC+TGG 0.485041 2.3:-3982572 MS.gene76157:CDS
GGATCTCCACATACTTTGCT+TGG 0.489007 2.3:+3982616 None:intergenic
AGGATTAGGGTTATCATCGT+TGG 0.489597 2.3:+3984368 None:intergenic
ACCTAGGCCACCTTGTGGAT+TGG 0.490198 2.3:+3982538 None:intergenic
TCGTTTCAAGATGATGCCCC+CGG 0.496104 2.3:-3984478 None:intergenic
AAAATGCTCCAGGTTCTACT+CGG 0.500819 2.3:-3983173 MS.gene76157:intron
TTAGATTCATCGAATTCGAT+AGG 0.502616 2.3:+3984342 None:intergenic
TTGAATTTCAGGTTATTAGT+GGG 0.502752 2.3:-3983410 MS.gene76157:intron
CGCAAACATGGACATTGTAA+AGG 0.507879 2.3:+3984238 None:intergenic
TATATCATGTTATAGGAAGA+AGG 0.515481 2.3:-3983017 MS.gene76157:intron
AAATGCTCCAGGTTCTACTC+GGG 0.515947 2.3:-3983172 MS.gene76157:intron
TGAATATCGCTCTCAATATG+AGG 0.517882 2.3:+3981665 None:intergenic
TTTGATTCATCTTGTTGTGA+AGG 0.524748 2.3:+3984408 None:intergenic
TCAGGGTGCAGCTCGTCGGT+TGG 0.528374 2.3:-3982361 MS.gene76157:intron
TTGTTCAGGGTGCAGCTCGT+CGG 0.533664 2.3:-3982365 MS.gene76157:intron
TTACAGACCTTGTTGCTCAA+AGG 0.534339 2.3:+3982863 None:intergenic
GTTGATAAATGCAGCTATCA+GGG 0.535579 2.3:-3982945 MS.gene76157:CDS
TTGAGCAGAACAAGCTTCCA+CGG 0.545035 2.3:+3984043 None:intergenic
CTCCATCGCTGTTTGTGAGA+AGG 0.546172 2.3:+3984310 None:intergenic
GAAGAACGAGAGCTTCAACT+AGG 0.548339 2.3:+3984265 None:intergenic
ATATGAGGAGAAAGAGATAC+CGG 0.550193 2.3:+3981680 None:intergenic
TTTAATGATACTGATAATGA+TGG 0.552788 2.3:+3983109 None:intergenic
TGTTTAATTTACTGTCCACC+AGG 0.555593 2.3:+3981608 None:intergenic
TAGATTCATCGAATTCGATA+GGG 0.558614 2.3:+3984343 None:intergenic
GGTTGTTGCTGGAGCTAGGG+AGG 0.559179 2.3:-3983149 MS.gene76157:CDS
AATTGACCAAGCAAAGTATG+TGG 0.559860 2.3:-3982622 MS.gene76157:CDS
GATTACAGAGCACTTGAAAG+TGG 0.567031 2.3:-3981878 MS.gene76157:CDS
ATTTCTGCTGTGATTCCTGG+TGG 0.569344 2.3:-3981623 MS.gene76157:CDS
GCCAATGAAACACAATCCGC+AGG 0.578293 2.3:+3983850 None:intergenic
AAGATTGGAAACGAGGCCCG+GGG 0.581766 2.3:+3984461 None:intergenic
CATTTCTTGGCCAATCCACA+AGG 0.583050 2.3:-3982548 MS.gene76157:CDS
GAAACAGGTTGCAGTGACTC+CGG 0.585349 2.3:-3981699 MS.gene76157:intron
CAGATCACCTTTGAGCAACA+AGG 0.588553 2.3:-3982870 MS.gene76157:intron
ATCCTTCTCACAAACAGCGA+TGG 0.597000 2.3:-3984312 MS.gene76157:CDS
AAGTATACTTACAGCTGCAG+AGG 0.601559 2.3:+3982487 None:intergenic
ACGATTGAGGAACGCAAACA+TGG 0.602918 2.3:+3984226 None:intergenic
TCAACTAGGTGTGTGAGACC+TGG 0.606431 2.3:+3984279 None:intergenic
CTTCTTGAAGATTGGAAACG+AGG 0.607887 2.3:+3984454 None:intergenic
CTGATAGCTGCATTTATCAA+CGG 0.609132 2.3:+3982947 None:intergenic
ACCTGAAATCTCAAAAGCAG+TGG 0.609300 2.3:+3984010 None:intergenic
AACAGCTAACCAGTTTGGAG+TGG 0.612572 2.3:+3982595 None:intergenic
AATGCTCCAGGTTCTACTCG+GGG 0.618010 2.3:-3983171 MS.gene76157:intron
TTGGCCAAGAAATGACCAGT+TGG 0.621148 2.3:+3982557 None:intergenic
GTTATTAGTGGGATTAATCG+GGG 0.623644 2.3:-3983399 MS.gene76157:CDS
GAAGAAATACTTCAGACAAG+AGG 0.628902 2.3:-3982268 MS.gene76157:intron
TTACAGCTGCAGAGGCATCT+CGG 0.632794 2.3:+3982495 None:intergenic
TGTTGCTCAAAGGTGATCTG+GGG 0.633147 2.3:+3982873 None:intergenic
AGCGATGGAGTTGAATCACC+AGG 0.636526 2.3:-3984297 MS.gene76157:CDS
TAATAATTCAGGGACTCCTG+CGG 0.636885 2.3:-3983866 MS.gene76157:intron
TGAAGACCTAGGCCACCTTG+TGG 0.642303 2.3:+3982533 None:intergenic
AGAAATGACCAGTTGGATAG+CGG 0.646911 2.3:+3982564 None:intergenic
ATGCTCCAGGTTCTACTCGG+GGG 0.678166 2.3:-3983170 MS.gene76157:intron
TTCTTGGCCAATCCACAAGG+TGG 0.682503 2.3:-3982545 MS.gene76157:CDS
TGTTACGATTCGAGAAACCG+TGG 0.686133 2.3:-3984060 MS.gene76157:CDS
ATTAATCGGGGATCAAGCTG+CGG 0.696145 2.3:-3983387 MS.gene76157:CDS
GCTAGGGAGGCATTATTATG+CGG 0.712293 2.3:-3983136 MS.gene76157:CDS
GCTGTGCAAACTGAAGACCT+AGG 0.716901 2.3:+3982522 None:intergenic
AGATTGGAAACGAGGCCCGG+GGG 0.763136 2.3:+3984462 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAGTAATTTATAATAATTCA+GGG - chr2.3:3982208-3982227 MS.gene76157:intron 10.0%
!! TTAGTAATTTATAATAATTC+AGG - chr2.3:3982207-3982226 MS.gene76157:intron 10.0%
!!! AATCTGAAATTTTTGTTTTT+TGG - chr2.3:3982123-3982142 MS.gene76157:intron 15.0%
!!! ATCTGAAATTTTTGTTTTTT+GGG - chr2.3:3982124-3982143 MS.gene76157:intron 15.0%
!! ACATATAGAATATTACAAGA+CGG + chr2.3:3982811-3982830 None:intergenic 20.0%
!! TGATTATGTAAATTTGATTC+TGG - chr2.3:3982375-3982394 MS.gene76157:intron 20.0%
!! TTCAAAATCAATTCTTGTTA+CGG + chr2.3:3982350-3982369 None:intergenic 20.0%
!! TTCATATATTGTACAAAATC+TGG - chr2.3:3983326-3983345 MS.gene76157:intron 20.0%
!! TTTAACTATATTGTTCTTGA+TGG - chr2.3:3984278-3984297 MS.gene76157:CDS 20.0%
!!! AATATTCAGAAAGCAATTTT+TGG + chr2.3:3983186-3983205 None:intergenic 20.0%
!!! ATATTCAGAAAGCAATTTTT+GGG + chr2.3:3983185-3983204 None:intergenic 20.0%
!!! TCAAGTTAGAATAATTTTGA+AGG - chr2.3:3982518-3982537 MS.gene76157:CDS 20.0%
!!! TCTGAAATTTTTGTTTTTTG+GGG - chr2.3:3982125-3982144 MS.gene76157:intron 20.0%
!!! TTTAATGATACTGATAATGA+TGG + chr2.3:3982978-3982997 None:intergenic 20.0%
!!! TTTCATTTGAGATTATGAAT+TGG - chr2.3:3983039-3983058 MS.gene76157:intron 20.0%
! AAACACAAATCTCTATAACA+AGG + chr2.3:3982879-3982898 None:intergenic 25.0%
! CATTATCAGTATCATTAAAC+TGG - chr2.3:3982979-3982998 MS.gene76157:CDS 25.0%
! GGTATCATATATCATGTTAT+AGG - chr2.3:3983060-3983079 MS.gene76157:intron 25.0%
! GTCTTGATTAGAATAGAATT+TGG - chr2.3:3983868-3983887 MS.gene76157:intron 25.0%
! TAATAAATGCTAACCAAAAC+AGG + chr2.3:3982291-3982310 None:intergenic 25.0%
! TATATCATGTTATAGGAAGA+AGG - chr2.3:3983067-3983086 MS.gene76157:intron 25.0%
!! ATTTTTGTTAGTTGCTGAAA+TGG - chr2.3:3981966-3981985 MS.gene76157:intron 25.0%
!! GAAGTATTTCTTCACTTTAT+TGG + chr2.3:3983809-3983828 None:intergenic 25.0%
!! GGTAATATCTTTGACTTTAT+GGG - chr2.3:3984299-3984318 MS.gene76157:CDS 25.0%
!! TGGTAATATCTTTGACTTTA+TGG - chr2.3:3984298-3984317 MS.gene76157:CDS 25.0%
!!! TATACCTCTAGAATCAATTT+TGG - chr2.3:3982588-3982607 MS.gene76157:CDS 25.0%
!!! TTGAATTTCAGGTTATTAGT+GGG - chr2.3:3982674-3982693 MS.gene76157:intron 25.0%
!!! TTTGAATTTCAGGTTATTAG+TGG - chr2.3:3982673-3982692 MS.gene76157:intron 25.0%
ACAATGCAGTAAAACATATG+GGG + chr2.3:3983278-3983297 None:intergenic 30.0%
ACATGATGTTGTTGTATGAA+GGG + chr2.3:3984066-3984085 None:intergenic 30.0%
ACATGTAAGTTGCTAACTTA+TGG - chr2.3:3982725-3982744 MS.gene76157:intron 30.0%
AGGTTATTAGTGGGATTAAT+CGG - chr2.3:3982683-3982702 MS.gene76157:intron 30.0%
CAAACACGCAATTAATTTAC+GGG + chr2.3:3982321-3982340 None:intergenic 30.0%
CACAATGCAGTAAAACATAT+GGG + chr2.3:3983279-3983298 None:intergenic 30.0%
CATGATGTGATTTGAATTTC+AGG - chr2.3:3982663-3982682 MS.gene76157:intron 30.0%
CCAAACACGCAATTAATTTA+CGG + chr2.3:3982322-3982341 None:intergenic 30.0%
GAATATTACAAGACGGAAAA+TGG + chr2.3:3982804-3982823 None:intergenic 30.0%
GATACTTTCTGTCTATTCTT+AGG - chr2.3:3983646-3983665 MS.gene76157:intron 30.0%
GTACAGAAGATAACAATTTG+TGG - chr2.3:3983939-3983958 MS.gene76157:intron 30.0%
GTATCATTAAACTGGTAAGT+TGG - chr2.3:3982987-3983006 MS.gene76157:CDS 30.0%
TAAGTTGCTAACTTATGGTT+GGG - chr2.3:3982730-3982749 MS.gene76157:intron 30.0%
TAGATTCATCGAATTCGATA+GGG + chr2.3:3981744-3981763 None:intergenic 30.0%
TATAACAAGGCTGCAAATTA+TGG + chr2.3:3982866-3982885 None:intergenic 30.0%
TGAAGTTCAATTTCAGAACT+CGG + chr2.3:3982772-3982791 None:intergenic 30.0%
TGTACAAAATCTGGTATTCT+AGG - chr2.3:3983335-3983354 MS.gene76157:intron 30.0%
TTAGATTCATCGAATTCGAT+AGG + chr2.3:3981745-3981764 None:intergenic 30.0%
TTCTTCACTTTATTGGTATC+AGG + chr2.3:3983802-3983821 None:intergenic 30.0%
TTGGAGATTATTGAATCAAC+TGG - chr2.3:3983763-3983782 MS.gene76157:intron 30.0%
TTTAGGGATAAATCTGTTTC+TGG - chr2.3:3981995-3982014 MS.gene76157:intron 30.0%
! AAGTCAAGAAACTGATTTCA+AGG - chr2.3:3983094-3983113 MS.gene76157:intron 30.0%
! AATGGTATCCTTTTATTGAC+AGG - chr2.3:3983433-3983452 MS.gene76157:intron 30.0%
! ATGGTATCCTTTTATTGACA+GGG - chr2.3:3983434-3983453 MS.gene76157:intron 30.0%
! TTTGATTCATCTTGTTGTGA+AGG + chr2.3:3981679-3981698 None:intergenic 30.0%
!! TTGAATCCATGTTTTGTTCA+GGG - chr2.3:3983706-3983725 MS.gene76157:intron 30.0%
AAAAAAAGAACGCACGATTG+AGG + chr2.3:3981874-3981893 None:intergenic 35.0%
AAATCAATTCTTGTTACGGC+AGG + chr2.3:3982346-3982365 None:intergenic 35.0%
AAGTTGCTAACTTATGGTTG+GGG - chr2.3:3982731-3982750 MS.gene76157:intron 35.0%
AATTCGATAGGGTTAGGATT+AGG + chr2.3:3981733-3981752 None:intergenic 35.0%
AATTGACCAAGCAAAGTATG+TGG - chr2.3:3983462-3983481 MS.gene76157:intron 35.0%
ACTTCAGACAAGAGGTAAAT+AGG - chr2.3:3983824-3983843 MS.gene76157:CDS 35.0%
AGAAATAGATGACGATGATC+TGG - chr2.3:3984184-3984203 MS.gene76157:intron 35.0%
ATATGAGGAGAAAGAGATAC+CGG + chr2.3:3984407-3984426 None:intergenic 35.0%
ATTCGATAGGGTTAGGATTA+GGG + chr2.3:3981732-3981751 None:intergenic 35.0%
CAATGCAGTAAAACATATGG+GGG + chr2.3:3983277-3983296 None:intergenic 35.0%
CACATGATGTTGTTGTATGA+AGG + chr2.3:3984067-3984086 None:intergenic 35.0%
CCGTAAATTAATTGCGTGTT+TGG - chr2.3:3982319-3982338 MS.gene76157:CDS 35.0%
CTGATAGCTGCATTTATCAA+CGG + chr2.3:3983140-3983159 None:intergenic 35.0%
CTGTTTGTGAGAAGGATAAT+TGG + chr2.3:3981769-3981788 None:intergenic 35.0%
GAAGAAATACTTCAGACAAG+AGG - chr2.3:3983816-3983835 MS.gene76157:CDS 35.0%
GCAAAGAACAGTATAACTCA+TGG + chr2.3:3984022-3984041 None:intergenic 35.0%
GCACAATGCAGTAAAACATA+TGG + chr2.3:3983280-3983299 None:intergenic 35.0%
GGTTATTAGTGGGATTAATC+GGG - chr2.3:3982684-3982703 MS.gene76157:intron 35.0%
GTAAGTTGCTAACTTATGGT+TGG - chr2.3:3982729-3982748 MS.gene76157:intron 35.0%
GTTATTAGTGGGATTAATCG+GGG - chr2.3:3982685-3982704 MS.gene76157:intron 35.0%
TACTGTTTACACTGATGCAA+TGG - chr2.3:3983415-3983434 MS.gene76157:intron 35.0%
TGAATATCGCTCTCAATATG+AGG + chr2.3:3984422-3984441 None:intergenic 35.0%
TTCAAACGACAACTTCTGAT+TGG - chr2.3:3984438-3984457 MS.gene76157:CDS 35.0%
! ATCATCGTTGGTTGAAGAAA+CGG + chr2.3:3981707-3981726 None:intergenic 35.0%
! CTACTTGTACCTTCAGTTTT+TGG - chr2.3:3984148-3984167 MS.gene76157:intron 35.0%
! GTAGCCAAAATTGATTCTAG+AGG + chr2.3:3982595-3982614 None:intergenic 35.0%
! GTTGATAAATGCAGCTATCA+GGG - chr2.3:3983139-3983158 MS.gene76157:CDS 35.0%
! TGTATGTGTGGTTTAACTTC+TGG + chr2.3:3982619-3982638 None:intergenic 35.0%
! TTTGAAACAGCTAACCAGTT+TGG + chr2.3:3983497-3983516 None:intergenic 35.0%
! TTTGAAGCCCTGTCAATAAA+AGG + chr2.3:3983444-3983463 None:intergenic 35.0%
!! AGTTGGTGATTCTATACATC+TGG - chr2.3:3983004-3983023 MS.gene76157:CDS 35.0%
!! ATCTGGTATTCTAGGACATT+TGG - chr2.3:3983343-3983362 MS.gene76157:intron 35.0%
!! CTTGAATCCATGTTTTGTTC+AGG - chr2.3:3983705-3983724 MS.gene76157:intron 35.0%
!! TGTGTTTCATTGGCTTTATC+TGG - chr2.3:3982243-3982262 MS.gene76157:intron 35.0%
AAAATGCTCCAGGTTCTACT+CGG - chr2.3:3982911-3982930 MS.gene76157:CDS 40.0%
AAGAGGTAAATAGGAGGATG+AGG - chr2.3:3983833-3983852 MS.gene76157:CDS 40.0%
AAGTATACTTACAGCTGCAG+AGG + chr2.3:3983600-3983619 None:intergenic 40.0%
ACCTGAAATCTCAAAAGCAG+TGG + chr2.3:3982077-3982096 None:intergenic 40.0%
AGAAATGACCAGTTGGATAG+CGG + chr2.3:3983523-3983542 None:intergenic 40.0%
AGGATTAGGGTTATCATCGT+TGG + chr2.3:3981719-3981738 None:intergenic 40.0%
ATGAATCCGTCAGAATGAGA+AGG + chr2.3:3984351-3984370 None:intergenic 40.0%
CAGCACTATTAGTGTATGTG+TGG + chr2.3:3982631-3982650 None:intergenic 40.0%
CGCAAACATGGACATTGTAA+AGG + chr2.3:3981849-3981868 None:intergenic 40.0%
CTATCCAACTGGTCATTTCT+TGG - chr2.3:3983523-3983542 MS.gene76157:intron 40.0%
CTTCTTGAAGATTGGAAACG+AGG + chr2.3:3981633-3981652 None:intergenic 40.0%
GAATGAGAAGGGAGCAAAAT+TGG + chr2.3:3984339-3984358 None:intergenic 40.0%
GCTTGTTCTGCTCAAGTTAA+TGG - chr2.3:3982049-3982068 MS.gene76157:intron 40.0%
GGCTACGCAGAAAAAGAATT+TGG - chr2.3:3983744-3983763 MS.gene76157:intron 40.0%
TAAATAGGAGGATGAGGATG+AGG - chr2.3:3983839-3983858 MS.gene76157:CDS 40.0%
TAATAATTCAGGGACTCCTG+CGG - chr2.3:3982218-3982237 MS.gene76157:intron 40.0%
TCAGACAAGAGGTAAATAGG+AGG - chr2.3:3983827-3983846 MS.gene76157:CDS 40.0%
TCATCGAATTCGATAGGGTT+AGG + chr2.3:3981739-3981758 None:intergenic 40.0%
TGAATCCGTCAGAATGAGAA+GGG + chr2.3:3984350-3984369 None:intergenic 40.0%
TTACAGACCTTGTTGCTCAA+AGG + chr2.3:3983224-3983243 None:intergenic 40.0%
TTGTTGCTCAAAGGTGATCT+GGG + chr2.3:3983215-3983234 None:intergenic 40.0%
! CAACTTGCTTTCACACATGA+AGG + chr2.3:3982441-3982460 None:intergenic 40.0%
! CGTTGATAAATGCAGCTATC+AGG - chr2.3:3983138-3983157 MS.gene76157:CDS 40.0%
! GATTACAGAGCACTTGAAAG+TGG - chr2.3:3984206-3984225 MS.gene76157:intron 40.0%
!! GAGAAGTGCTTCTTGAAGAT+TGG + chr2.3:3981641-3981660 None:intergenic 40.0%
!! GCTGAAATGGTGTTGCTTTA+GGG - chr2.3:3981979-3981998 MS.gene76157:intron 40.0%
!! TGCTGAAATGGTGTTGCTTT+AGG - chr2.3:3981978-3981997 MS.gene76157:intron 40.0%
!!! ACCTGGAGCATTTTAAAGCA+TGG + chr2.3:3982905-3982924 None:intergenic 40.0%
!!! TATCTGGAGCTTTGTTTTCG+TGG - chr2.3:3982259-3982278 MS.gene76157:intron 40.0%
AAATGCTCCAGGTTCTACTC+GGG - chr2.3:3982912-3982931 MS.gene76157:CDS 45.0%
AACAGCTAACCAGTTTGGAG+TGG + chr2.3:3983492-3983511 None:intergenic 45.0%
ACGATTGAGGAACGCAAACA+TGG + chr2.3:3981861-3981880 None:intergenic 45.0%
ACGGATTCATGCATGTGCTT+TGG - chr2.3:3984361-3984380 MS.gene76157:CDS 45.0%
AGCTATCAGGGATGCTGAAA+AGG - chr2.3:3983151-3983170 MS.gene76157:CDS 45.0%
ATCCTTCTCACAAACAGCGA+TGG - chr2.3:3981772-3981791 MS.gene76157:intron 45.0%
ATTAATCGGGGATCAAGCTG+CGG - chr2.3:3982697-3982716 MS.gene76157:intron 45.0%
ATTGAATCAACTGGAGCTGC+AGG - chr2.3:3983772-3983791 MS.gene76157:intron 45.0%
ATTTCTGCTGTGATTCCTGG+TGG - chr2.3:3984461-3984480 MS.gene76157:CDS 45.0%
CAGATCACCTTTGAGCAACA+AGG - chr2.3:3983214-3983233 MS.gene76157:intron 45.0%
CATTTCTTGGCCAATCCACA+AGG - chr2.3:3983536-3983555 MS.gene76157:intron 45.0%
CTTGTTGCTCAAAGGTGATC+TGG + chr2.3:3983216-3983235 None:intergenic 45.0%
GAAGAACGAGAGCTTCAACT+AGG + chr2.3:3981822-3981841 None:intergenic 45.0%
GCTGCTTGTCCAAAAACTGA+AGG + chr2.3:3984160-3984179 None:intergenic 45.0%
GGATCTCCACATACTTTGCT+TGG + chr2.3:3983471-3983490 None:intergenic 45.0%
TCAAATAGCCGCTATCCAAC+TGG - chr2.3:3983512-3983531 MS.gene76157:intron 45.0%
TGGATTTCTGCTGTGATTCC+TGG - chr2.3:3984458-3984477 MS.gene76157:CDS 45.0%
TGTTACGATTCGAGAAACCG+TGG - chr2.3:3982024-3982043 MS.gene76157:intron 45.0%
TGTTGCTCAAAGGTGATCTG+GGG + chr2.3:3983214-3983233 None:intergenic 45.0%
TTGAGCAGAACAAGCTTCCA+CGG + chr2.3:3982044-3982063 None:intergenic 45.0%
TTGCTCCCTTCTCATTCTGA+CGG - chr2.3:3984342-3984361 MS.gene76157:CDS 45.0%
TTGGCCAAGAAATGACCAGT+TGG + chr2.3:3983530-3983549 None:intergenic 45.0%
! TCCTGCGGATTGTGTTTCAT+TGG - chr2.3:3982233-3982252 MS.gene76157:intron 45.0%
! TTCGTGGTCAAAGCCTGTTT+TGG - chr2.3:3982275-3982294 MS.gene76157:CDS 45.0%
!! GCCATGCTTTAAAATGCTCC+AGG - chr2.3:3982901-3982920 MS.gene76157:CDS 45.0%
!! GCTAGGGAGGCATTATTATG+CGG - chr2.3:3982948-3982967 MS.gene76157:CDS 45.0%
!! TCAGTTTTTGGACAAGCAGC+AGG - chr2.3:3984160-3984179 MS.gene76157:intron 45.0%
!! TGCATGTGCTTTGGAGAAAC+AGG - chr2.3:3984370-3984389 MS.gene76157:CDS 45.0%
!!! GCCACTGCTTTTGAGATTTC+AGG - chr2.3:3982073-3982092 MS.gene76157:intron 45.0%
AATGCTCCAGGTTCTACTCG+GGG - chr2.3:3982913-3982932 MS.gene76157:CDS 50.0%
AGCGATGGAGTTGAATCACC+AGG - chr2.3:3981787-3981806 MS.gene76157:intron 50.0%
ATAGGAGGATGAGGATGAGG+AGG - chr2.3:3983842-3983861 MS.gene76157:CDS 50.0%
CTCCATCGCTGTTTGTGAGA+AGG + chr2.3:3981777-3981796 None:intergenic 50.0%
GAAACAGGTTGCAGTGACTC+CGG - chr2.3:3984385-3984404 MS.gene76157:CDS 50.0%
GCCAATGAAACACAATCCGC+AGG + chr2.3:3982237-3982256 None:intergenic 50.0%
GCTGCACCCTGAACAAAACA+TGG + chr2.3:3983715-3983734 None:intergenic 50.0%
GCTGTGCAAACTGAAGACCT+AGG + chr2.3:3983565-3983584 None:intergenic 50.0%
GGTCTTCAGTTTGCACAGCT+TGG - chr2.3:3983566-3983585 MS.gene76157:intron 50.0%
TAGGAGGATGAGGATGAGGA+GGG - chr2.3:3983843-3983862 MS.gene76157:CDS 50.0%
TCAACTAGGTGTGTGAGACC+TGG + chr2.3:3981808-3981827 None:intergenic 50.0%
TGAAGATTGGAAACGAGGCC+CGG + chr2.3:3981628-3981647 None:intergenic 50.0%
TGTGGAGATCCACTCCAAAC+TGG - chr2.3:3983480-3983499 MS.gene76157:intron 50.0%
TTCTTGGCCAATCCACAAGG+TGG - chr2.3:3983539-3983558 MS.gene76157:intron 50.0%
!! TTACAGCTGCAGAGGCATCT+CGG + chr2.3:3983592-3983611 None:intergenic 50.0%
AACAACCCCCGAGTAGAACC+TGG + chr2.3:3982922-3982941 None:intergenic 55.0%
AAGATTGGAAACGAGGCCCG+GGG + chr2.3:3981626-3981645 None:intergenic 55.0%
ACCTAGGCCACCTTGTGGAT+TGG + chr2.3:3983549-3983568 None:intergenic 55.0%
AGGAGGATGAGGATGAGGAG+GGG - chr2.3:3983844-3983863 MS.gene76157:CDS 55.0%
ATGCTCCAGGTTCTACTCGG+GGG - chr2.3:3982914-3982933 MS.gene76157:CDS 55.0%
GAAGATTGGAAACGAGGCCC+GGG + chr2.3:3981627-3981646 None:intergenic 55.0%
TGAAGACCTAGGCCACCTTG+TGG + chr2.3:3983554-3983573 None:intergenic 55.0%
TTCTACTCGGGGGTTGTTGC+TGG - chr2.3:3982924-3982943 MS.gene76157:CDS 55.0%
TTGTTCAGGGTGCAGCTCGT+CGG - chr2.3:3983719-3983738 MS.gene76157:intron 55.0%
AGATTGGAAACGAGGCCCGG+GGG + chr2.3:3981625-3981644 None:intergenic 60.0%
GAGGATGAGGATGAGGAGGG+GGG - chr2.3:3983846-3983865 MS.gene76157:CDS 60.0%
GCCAATCCACAAGGTGGCCT+AGG - chr2.3:3983545-3983564 MS.gene76157:intron 60.0%
GGAGGATGAGGATGAGGAGG+GGG - chr2.3:3983845-3983864 MS.gene76157:CDS 60.0%
GGGGGTTGTTGCTGGAGCTA+GGG - chr2.3:3982932-3982951 MS.gene76157:CDS 60.0%
GGTTGTTGCTGGAGCTAGGG+AGG - chr2.3:3982935-3982954 MS.gene76157:CDS 60.0%
CGGGGGTTGTTGCTGGAGCT+AGG - chr2.3:3982931-3982950 MS.gene76157:CDS 65.0%
TCAGGGTGCAGCTCGTCGGT+TGG - chr2.3:3983723-3983742 MS.gene76157:intron 65.0%


Chromosome Type Strat End Strand Name
chr2.3 gene 3981616 3984490 3981616 ID=MS.gene76157
chr2.3 mRNA 3981616 3984490 3981616 ID=MS.gene76157.t1;Parent=MS.gene76157
chr2.3 exon 3984228 3984490 3984228 ID=MS.gene76157.t1.exon1;Parent=MS.gene76157.t1
chr2.3 CDS 3984228 3984490 3984228 ID=cds.MS.gene76157.t1;Parent=MS.gene76157.t1
chr2.3 exon 3984012 3984106 3984012 ID=MS.gene76157.t1.exon2;Parent=MS.gene76157.t1
chr2.3 CDS 3984012 3984106 3984012 ID=cds.MS.gene76157.t1;Parent=MS.gene76157.t1
chr2.3 exon 3983810 3983877 3983810 ID=MS.gene76157.t1.exon3;Parent=MS.gene76157.t1
chr2.3 CDS 3983810 3983877 3983810 ID=cds.MS.gene76157.t1;Parent=MS.gene76157.t1
chr2.3 exon 3983378 3983421 3983378 ID=MS.gene76157.t1.exon4;Parent=MS.gene76157.t1
chr2.3 CDS 3983378 3983421 3983378 ID=cds.MS.gene76157.t1;Parent=MS.gene76157.t1
chr2.3 exon 3983106 3983183 3983106 ID=MS.gene76157.t1.exon5;Parent=MS.gene76157.t1
chr2.3 CDS 3983106 3983183 3983106 ID=cds.MS.gene76157.t1;Parent=MS.gene76157.t1
chr2.3 exon 3982871 3983024 3982871 ID=MS.gene76157.t1.exon6;Parent=MS.gene76157.t1
chr2.3 CDS 3982871 3983024 3982871 ID=cds.MS.gene76157.t1;Parent=MS.gene76157.t1
chr2.3 exon 3982499 3982651 3982499 ID=MS.gene76157.t1.exon7;Parent=MS.gene76157.t1
chr2.3 CDS 3982499 3982651 3982499 ID=cds.MS.gene76157.t1;Parent=MS.gene76157.t1
chr2.3 exon 3982269 3982379 3982269 ID=MS.gene76157.t1.exon8;Parent=MS.gene76157.t1
chr2.3 CDS 3982269 3982379 3982269 ID=cds.MS.gene76157.t1;Parent=MS.gene76157.t1
chr2.3 exon 3981874 3981942 3981874 ID=MS.gene76157.t1.exon9;Parent=MS.gene76157.t1
chr2.3 CDS 3981874 3981942 3981874 ID=cds.MS.gene76157.t1;Parent=MS.gene76157.t1
chr2.3 exon 3981616 3981714 3981616 ID=MS.gene76157.t1.exon10;Parent=MS.gene76157.t1
chr2.3 CDS 3981616 3981714 3981616 ID=cds.MS.gene76157.t1;Parent=MS.gene76157.t1
Gene Sequence

>MS.gene76157

ATGATGCCCCCGGGCCTCGTTTCCAATCTTCAAGAAGCACTTCTCAACAGAAAAATCACACCTTCACAACAAGATGAATCAAACGACACCGTTTCTTCAACCAACGATGATAACCCTAATCCTAACCCTATCGAATTCGATGAATCTAAACCAATTATCCTTCTCACAAACAGCGATGGAGTTGAATCACCAGGTCTCACACACCTAGTTGAAGCTCTCGTTCTTCAAAACCTTTACAATGTCCATGTTTGCGTTCCTCAATCGTGCGTTCTTTTTTTAATCTGTTAAGAAATGAAGTAAATTTTTGATTTTTTTTTTGTTGTTATTGAATAATGTTGAATGTGTTTGTAATTTTTGTTAGTTGCTGAAATGGTGTTGCTTTAGGGATAAATCTGTTTCTGGTCATTCTGTTACGATTCGAGAAACCGTGGAAGCTTGTTCTGCTCAAGTTAATGGTGCCACTGCTTTTGAGATTTCAGGTATTAACTTCATTCAATTCTGTTTCTAAATCTGAAATTTTTGTTTTTTGGGGTGTGCTTATGAATTTTTTGATATTAATAATGTAATATGAATTTGTTAACGTTTTCTAATTTAGTAATTTATAATAATTCAGGGACTCCTGCGGATTGTGTTTCATTGGCTTTATCTGGAGCTTTGTTTTCGTGGTCAAAGCCTGTTTTGGTTAGCATTTATTATCTTACACCCGTAAATTAATTGCGTGTTTGGTCCTGCCGTAACAAGAATTGATTTTGAATAAATTGATTATGTAAATTTGATTCTGGCTTAAAGTGAGTTGAAAGTAAAATTATTTATGTTTGAATACCTTCATGTGTGAAAGCAAGTTGAACGATAAATTTGAGACTAGAAATCAGTTCTAGAATCAAAAGCTTCAAATTCTAGCTTCAAGTTAGAATAATTTTGAAGGCAGAATCAATTATACTCGAGAGCAATCAAACATGTCAAAATCAATTCTATACCTCTAGAATCAATTTTGGCTACTCCAGAAGTTAAACCACACATACACTAATAGTGCTGTGATATAGTAATCATGATGTGATTTGAATTTCAGGTTATTAGTGGGATTAATCGGGGATCAAGCTGCGGTCATCACATGTAAGTTGCTAACTTATGGTTGGGGAGAAGTTATATAAATCCGAGTTCTGAAATTGAACTTCAACACATTTTCCATTTTCCGTCTTGTAATATTCTATATGTATTGTTTTTGTTTTAAGATTTGTATCTATTGTCCATAATTTGCAGCCTTGTTATAGAGATTTGTGTTTATGCCATGCTTTAAAATGCTCCAGGTTCTACTCGGGGGTTGTTGCTGGAGCTAGGGAGGCATTATTATGCGGCGTACCATCATTATCAGTATCATTAAACTGGTAAGTTGGTGATTCTATACATCTGGTTTTAACTATGCTTTCATTTGAGATTATGAATTGGTATCATATATCATGTTATAGGAAGAAGGATGAAAGTCAAGAAACTGATTTCAAGGATGCAGTTGAAGTCTGTTTACCGTTGATAAATGCAGCTATCAGGGATGCTGAAAAGGAAACATTTCCCAAAAATTGCTTTCTGAATATTGAAATCCCCAGATCACCTTTGAGCAACAAGGTCTGTAAATTTGTTAAATAATAATTTGATTGTTTTTTCCCCCATATGTTTTACTGCATTGTGCAAATGCTCTAGTCTTCTAAGTTTTCTTTCATATATTGTACAAAATCTGGTATTCTAGGACATTTGGCTAATTGATTCTAATGATCAAATTATTTCATAGTTTTTTTTTTCTCGCATACTGTTTACACTGATGCAATGGTATCCTTTTATTGACAGGGCTTCAAATTGACCAAGCAAAGTATGTGGAGATCCACTCCAAACTGGTTAGCTGTTTCAAATAGCCGCTATCCAACTGGTCATTTCTTGGCCAATCCACAAGGTGGCCTAGGTCTTCAGTTTGCACAGCTTGGCCGAGATGCCTCTGCAGCTGTAAGTATACTTCATTACATACAAACATGTGTTATGTTGATACTTTCTGTCTATTCTTAGGTGTGATTTTAATGTGTTTAATCTATTGATGCTGATACTTGAATCCATGTTTTGTTCAGGGTGCAGCTCGTCGGTTGGCTACGCAGAAAAAGAATTTGGAGATTATTGAATCAACTGGAGCTGCAGGAAAACCTGATACCAATAAAGTGAAGAAATACTTCAGACAAGAGGTAAATAGGAGGATGAGGATGAGGAGGGGGGTCTTGATTAGAATAGAATTTGGTTTGTACAAATCTTGTTAATGTTGAAGTTAATCTGTTTTCAATGCTGAGTACAGAAGATAACAATTTGTGGAGAATAGATTATCATAGAAGTTATCTTCATACTTGAGAAGCTTTATCTAAGTTTTAACCATGAGTTATACTGTTCTTTGCATTTTAGTTTATTAATCTGTTCCCTTCATACAACAACATCATGTGTTATAGTTGTATTTCAAATAAGCAACATCAAATTATTTGTACAAGTGAACTTAGCTATAATCTACTTGTACCTTCAGTTTTTGGACAAGCAGCAGGAAGAAATAGATGACGATGATCTGGATTACAGAGCACTTGAAAGTGGATATGTAAGTTTGAGCAATAAATTATTTCTTCAATTGATATTAGTTATCTTTAACTATATTGTTCTTGATGGTAATATCTTTGACTTTATGGGCTGTCATACATTTACCAATTTTGCTCCCTTCTCATTCTGACGGATTCATGCATGTGCTTTGGAGAAACAGGTTGCAGTGACTCCGGTATCTCTTTCTCCTCATATTGAGAGCGATATTCAAACGACAACTTCTGATTGGATTTCTGCTGTGATTCCTGGTGGACAGTAA
Protein sequence

>MS.gene76157.t1

MMPPGLVSNLQEALLNRKITPSQQDESNDTVSSTNDDNPNPNPIEFDESKPIILLTNSDGVESPGLTHLVEALVLQNLYNVHVCVPQSDKSVSGHSVTIRETVEACSAQVNGATAFEISGTPADCVSLALSGALFSWSKPVLVISGINRGSSCGHHMFYSGVVAGAREALLCGVPSLSVSLNWKKDESQETDFKDAVEVCLPLINAAIRDAEKETFPKNCFLNIEIPRSPLSNKGFKLTKQSMWRSTPNWLAVSNSRYPTGHFLANPQGGLGLQFAQLGRDASAAGAARRLATQKKNLEIIESTGAAGKPDTNKVKKYFRQEFLDKQQEEIDDDDLDYRALESGYVAVTPVSLSPHIESDIQTTTSDWISAVIPGGQ