AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene057003

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene057003.t1 MTR_2g099720 80.585 376 27 5 1 330 2 377 0.0 586
MS.gene057003.t1 MTR_5g006290 32.479 234 118 5 96 325 101 298 2.25e-37 135
MS.gene057003.t1 MTR_1g104500 31.897 232 122 4 96 325 100 297 6.79e-35 129
MS.gene057003.t1 MTR_5g006290 46.903 113 60 0 96 208 101 213 6.83e-32 120
MS.gene057003.t1 MTR_5g006300 44.762 105 52 1 96 194 94 198 5.24e-26 105
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene057003.t1 AT1G72880 50.919 381 133 10 1 330 8 385 9.37e-115 337
MS.gene057003.t1 AT1G72880 50.919 381 133 10 1 330 8 385 9.37e-115 337
MS.gene057003.t1 AT4G14930 27.103 321 156 8 49 322 13 302 9.66e-29 113
MS.gene057003.t1 AT4G14930 33.171 205 90 4 49 206 13 217 2.40e-27 109

Find 84 sgRNAs with CRISPR-Local

Find 186 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTAGGGATAAATCTGTTTC+TGG 0.178353 2.1:-4926392 MS.gene057003:intron
GAAGTATTTCTTCACTTTAT+TGG 0.213458 2.1:+4924636 None:intergenic
TTCTACTCTGGGGTTGTTGC+TGG 0.231757 2.1:-4925486 MS.gene057003:CDS
TGGATTTCTGCTGTGATTCC+TGG 0.252539 2.1:-4923983 MS.gene057003:CDS
AATTCGATAGGGTTAGGATT+AGG 0.265411 2.1:+4926657 None:intergenic
CGTTGATAAATGCAGCTATC+AGG 0.280646 2.1:-4925272 MS.gene057003:CDS
TTCTTCACTTTATTGGTATC+AGG 0.291732 2.1:+4924643 None:intergenic
TATATCATGTTATAGGAAAA+AGG 0.296377 2.1:-4925343 MS.gene057003:intron
ATTCGATAGGGTTAGGATTA+GGG 0.300748 2.1:+4926658 None:intergenic
CTATCCAACTGGTCATTTCT+TGG 0.301621 2.1:-4924919 MS.gene057003:CDS
AGGTTATTAGTGGGATTAAT+CGG 0.308990 2.1:-4925698 MS.gene057003:CDS
GCCAATCCACAAGGTGGCCT+TGG 0.331528 2.1:-4924897 MS.gene057003:CDS
TTTGAAACAGCTAACCAGTT+TGG 0.357202 2.1:+4924948 None:intergenic
TTGGAGATTATTGAATCAAC+TGG 0.357915 2.1:-4924679 MS.gene057003:CDS
GCTGTTTGTGAGCAAGATAA+TGG 0.364803 2.1:+4926620 None:intergenic
TTCAAACGTCAACTTCTGAT+TGG 0.384107 2.1:-4924003 MS.gene057003:CDS
CATTATCAATATCATTAAAC+TGG 0.385947 2.1:-4925431 MS.gene057003:intron
TCATCGAATTCGATAGGGTT+AGG 0.387109 2.1:+4926651 None:intergenic
GAGAAGTGCTTCTTGAAGAT+TGG 0.399004 2.1:+4926749 None:intergenic
CTGGGGTTGTTGCTGGAGCT+AGG 0.415674 2.1:-4925479 MS.gene057003:CDS
TGGGGTTGTTGCTGGAGCTA+GGG 0.421199 2.1:-4925478 MS.gene057003:CDS
CTGTTTGTGAGCAAGATAAT+GGG 0.422044 2.1:+4926621 None:intergenic
GGTTATTAGTGGGATTAATC+GGG 0.422540 2.1:-4925697 MS.gene057003:CDS
GGTCTTCAGTTTGCACAGCT+TGG 0.423296 2.1:-4924876 MS.gene057003:CDS
TGAAGATTGGAAACGAGGCC+CGG 0.439122 2.1:+4926762 None:intergenic
TGTGAGCAAGATAATGGGTT+TGG 0.440916 2.1:+4926626 None:intergenic
AAGTCAAGAAACTGATTTCA+AGG 0.442158 2.1:-4925316 MS.gene057003:CDS
TTTGAATTTCAGGTTATTAG+TGG 0.444025 2.1:-4925708 MS.gene057003:intron
AGAAATAGATGACGATGATC+TGG 0.446591 2.1:-4924255 MS.gene057003:CDS
GCTTGTTCCGCTCAAGTTAA+CGG 0.451765 2.1:-4926338 MS.gene057003:CDS
GAAGATTGGAAACGAGGCCC+GGG 0.453439 2.1:+4926763 None:intergenic
TTTAATGATATTGATAATGA+TGG 0.457599 2.1:+4925435 None:intergenic
ATTGAATCAACTGGAGCTGC+AGG 0.468057 2.1:-4924670 MS.gene057003:CDS
AATGCTCCAGGTTCTACTCT+GGG 0.470332 2.1:-4925497 MS.gene057003:intron
AAATGCTCCAGGTTCTACTC+TGG 0.471886 2.1:-4925498 MS.gene057003:intron
AGCTATCAGGGATGCTGAAA+AGG 0.478095 2.1:-4925259 MS.gene057003:CDS
TGTGGAGATCCACTCCAAAC+TGG 0.479831 2.1:-4924962 MS.gene057003:CDS
TCAAATAGCCGCTATCCAAC+TGG 0.485041 2.1:-4924930 MS.gene057003:CDS
ACCAAGGCCACCTTGTGGAT+TGG 0.492533 2.1:+4924896 None:intergenic
TCGTTTCAAGATGATGCCCC+CGG 0.496104 2.1:-4926781 None:intergenic
AGGATTAGGGTTATCATCGT+TGG 0.497932 2.1:+4926671 None:intergenic
TTGTTCAGGGCGCAGCTCGT+CGG 0.499499 2.1:-4924723 MS.gene057003:intron
TTGAATTTCAGGTTATTAGT+GGG 0.502752 2.1:-4925707 MS.gene057003:intron
TTGGATTCATCGAATTCGAT+AGG 0.510175 2.1:+4926645 None:intergenic
TGAATATCACTCTCAATATG+AGG 0.515335 2.1:+4924022 None:intergenic
ATCATCGTTGGTGGAAGAAA+CGG 0.515938 2.1:+4926683 None:intergenic
TTGTTGCTCAAAGGTGATCT+CGG 0.524280 2.1:+4925198 None:intergenic
TTTGATTCATCTTGTTGTGA+AGG 0.524748 2.1:+4926711 None:intergenic
ATTAGGGTTATCATCGTTGG+TGG 0.527462 2.1:+4926674 None:intergenic
TTACAGACCTTGTTGCTCAA+AGG 0.534339 2.1:+4925189 None:intergenic
GGTTGTTGCTGGAGCTAGGG+AGG 0.535432 2.1:-4925475 MS.gene057003:CDS
GTTGATAAATGCAGCTATCA+GGG 0.535579 2.1:-4925271 MS.gene057003:CDS
TCAGGGCGCAGCTCGTCGGT+TGG 0.546235 2.1:-4924719 MS.gene057003:intron
TTGAGCGGAACAAGCTTCCA+CGG 0.547418 2.1:+4926346 None:intergenic
ATATGAGGAGAAAGAGATAC+CGG 0.550193 2.1:+4924037 None:intergenic
TGGATTCATCGAATTCGATA+GGG 0.554963 2.1:+4926646 None:intergenic
GATTACAGAGCACTTGAAAG+TGG 0.567031 2.1:-4924233 MS.gene057003:CDS
ATTTCTGCTGTGATTCCTGG+TGG 0.569344 2.1:-4923980 MS.gene057003:CDS
AAGATTGGAAACGAGGCCCG+GGG 0.581766 2.1:+4926764 None:intergenic
CATTTCTTGGCCAATCCACA+AGG 0.583050 2.1:-4924906 MS.gene057003:CDS
AATTGACTAAGCAAAGTATG+TGG 0.583954 2.1:-4924980 MS.gene057003:CDS
GAAACAGGTTGCAGTGACTC+CGG 0.585349 2.1:-4924056 MS.gene057003:intron
CTTCTTGAAGATTGGAAACG+AGG 0.607887 2.1:+4926757 None:intergenic
CTGATAGCTGCATTTATCAA+CGG 0.609132 2.1:+4925273 None:intergenic
ACCTGAAATCTCAAAAGCAG+TGG 0.609300 2.1:+4926313 None:intergenic
AACAGCTAACCAGTTTGGAG+TGG 0.612572 2.1:+4924953 None:intergenic
ATCTTGCTCACAAACAGCGA+TGG 0.615557 2.1:-4926615 MS.gene057003:CDS
TTGGCCAAGAAATGACCAGT+TGG 0.621148 2.1:+4924915 None:intergenic
TAGGATACTTACAGCTGCAG+AGG 0.621441 2.1:+4924845 None:intergenic
GTTATTAGTGGGATTAATCG+GGG 0.623644 2.1:-4925696 MS.gene057003:CDS
TTACAGCTGCAGAGGCATCT+CGG 0.632794 2.1:+4924853 None:intergenic
GAGATCACCTTTGAGCAACA+AGG 0.634652 2.1:-4925196 MS.gene057003:intron
AGCGATGGAGTTGAATCACC+AGG 0.636526 2.1:-4926600 MS.gene057003:intron
AGAAATGACCAGTTGGATAG+CGG 0.646911 2.1:+4924922 None:intergenic
GAAGAAATACTTCAGACTAG+AGG 0.658962 2.1:-4924626 MS.gene057003:intron
ATGCTCCAGGTTCTACTCTG+GGG 0.670311 2.1:-4925496 MS.gene057003:intron
AGTGGCACCGTTAACTTGAG+CGG 0.677136 2.1:+4926331 None:intergenic
TTCTTGGCCAATCCACAAGG+TGG 0.682503 2.1:-4924903 MS.gene057003:CDS
TGTTACGATTCGAGAAACCG+TGG 0.686133 2.1:-4926363 MS.gene057003:CDS
TGAAGACCAAGGCCACCTTG+TGG 0.695105 2.1:+4924891 None:intergenic
ATTAATCGGGGATCAAGCTG+CGG 0.696145 2.1:-4925684 MS.gene057003:CDS
GCTAGGGAGGCATTATTATG+CGG 0.703521 2.1:-4925462 MS.gene057003:CDS
GCTGTGCAAACTGAAGACCA+AGG 0.760862 2.1:+4924880 None:intergenic
AGATTGGAAACGAGGCCCGG+GGG 0.763136 2.1:+4926765 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAGTAATTTATAATAATTCA+GGG - chr2.1:4924569-4924588 MS.gene057003:intron 10.0%
!! TTAGTAATTTATAATAATTC+AGG - chr2.1:4924568-4924587 MS.gene057003:intron 10.0%
!!! AAATCAAATTTTTGTTTTTT+GGG - chr2.1:4924476-4924495 MS.gene057003:intron 10.0%
!!! TAAATCAAATTTTTGTTTTT+TGG - chr2.1:4924475-4924494 MS.gene057003:intron 10.0%
!!! TAAATTTTTGATTTTTTTGT+TGG - chr2.1:4924270-4924289 MS.gene057003:CDS 10.0%
!!! AATCAAATTTTTGTTTTTTG+GGG - chr2.1:4924477-4924496 MS.gene057003:intron 15.0%
!!! TTTAATGATATTGATAATGA+TGG + chr2.1:4925309-4925328 None:intergenic 15.0%
!! CATTATCAATATCATTAAAC+TGG - chr2.1:4925310-4925329 MS.gene057003:CDS 20.0%
!! GAACAGATTAATAATCTAAA+AGG + chr2.1:4926349-4926368 None:intergenic 20.0%
!! TATATCATGTTATAGGAAAA+AGG - chr2.1:4925398-4925417 MS.gene057003:intron 20.0%
!! TTTCATTAGAGATTATGAAT+TGG - chr2.1:4925370-4925389 MS.gene057003:intron 20.0%
!!! AATATTCAGAAAGCAATTTT+TGG + chr2.1:4925517-4925536 None:intergenic 20.0%
!!! ATATTCAGAAAGCAATTTTT+GGG + chr2.1:4925516-4925535 None:intergenic 20.0%
!!! ATCAAATTTTTGTTTTTTGG+GGG - chr2.1:4924478-4924497 MS.gene057003:intron 20.0%
!!! TCAAGTTAGAATAATTTTGA+AGG - chr2.1:4924879-4924898 MS.gene057003:CDS 20.0%
!!! TGATTATGTAAAATTGATTC+TGG - chr2.1:4924736-4924755 MS.gene057003:intron 20.0%
! AAACACAAATCTCTATAACA+AGG + chr2.1:4925210-4925229 None:intergenic 25.0%
! AACACATAATGTTGTATGAA+GGG + chr2.1:4926371-4926390 None:intergenic 25.0%
! ACTATATTGTATGTTCTTGA+TGG - chr2.1:4926584-4926603 MS.gene057003:intron 25.0%
! ATATCATTAAACTGGTAAGT+TGG - chr2.1:4925318-4925337 MS.gene057003:CDS 25.0%
! CAAACACACAATTAATTTAC+GGG + chr2.1:4924682-4924701 None:intergenic 25.0%
! CCAAACACACAATTAATTTA+CGG + chr2.1:4924683-4924702 None:intergenic 25.0%
! GAATATTACAAGACAGAAAA+TGG + chr2.1:4925135-4925154 None:intergenic 25.0%
! GGTATCATATATCATGTTAT+AGG - chr2.1:4925391-4925410 MS.gene057003:intron 25.0%
! TAACACATAATGTTGTATGA+AGG + chr2.1:4926372-4926391 None:intergenic 25.0%
! TAATAAATGCTAACCAAAAC+AGG + chr2.1:4924652-4924671 None:intergenic 25.0%
!! ATTTTTGTTAGTTGCTGAAA+TGG - chr2.1:4924320-4924339 MS.gene057003:intron 25.0%
!! CTTGATGGTAATATCTTTAT+GGG - chr2.1:4926599-4926618 MS.gene057003:intron 25.0%
!! GAAGTATTTCTTCACTTTAT+TGG + chr2.1:4926108-4926127 None:intergenic 25.0%
!! TCTTGATGGTAATATCTTTA+TGG - chr2.1:4926598-4926617 MS.gene057003:intron 25.0%
!!! TTGAATTTCAGGTTATTAGT+GGG - chr2.1:4925034-4925053 MS.gene057003:intron 25.0%
!!! TTTGAATTTCAGGTTATTAG+TGG - chr2.1:4925033-4925052 MS.gene057003:intron 25.0%
AAGTTGCTAACTTATGGTTA+GGG - chr2.1:4925091-4925110 MS.gene057003:intron 30.0%
AATTGACTAAGCAAAGTATG+TGG - chr2.1:4925761-4925780 MS.gene057003:intron 30.0%
ACATGTAAGTTGCTAACTTA+TGG - chr2.1:4925085-4925104 MS.gene057003:intron 30.0%
AGATAACAATTTGTGGAGAA+TGG - chr2.1:4926251-4926270 MS.gene057003:intron 30.0%
AGGTTATTAGTGGGATTAAT+CGG - chr2.1:4925043-4925062 MS.gene057003:intron 30.0%
CACAATGCAGTAAAACATAT+GGG + chr2.1:4925613-4925632 None:intergenic 30.0%
CACATGTTTGTATGTAATGT+AGG + chr2.1:4925918-4925937 None:intergenic 30.0%
CATGATGTGATTTGAATTTC+AGG - chr2.1:4925023-4925042 MS.gene057003:intron 30.0%
CCGTAAATTAATTGTGTGTT+TGG - chr2.1:4924680-4924699 MS.gene057003:CDS 30.0%
GTACAGAAGATAACAATTTG+TGG - chr2.1:4926244-4926263 MS.gene057003:intron 30.0%
TAAGTTGCTAACTTATGGTT+AGG - chr2.1:4925090-4925109 MS.gene057003:intron 30.0%
TATAACAAGGCTGCAAATTA+TGG + chr2.1:4925197-4925216 None:intergenic 30.0%
TCTAACTTGAAGCTAGAATT+TGG + chr2.1:4924869-4924888 None:intergenic 30.0%
TCTTTCAATTGTACAAACTC+TGG - chr2.1:4925657-4925676 MS.gene057003:intron 30.0%
TGAATATCACTCTCAATATG+AGG + chr2.1:4926722-4926741 None:intergenic 30.0%
TTCTTCACTTTATTGGTATC+AGG + chr2.1:4926101-4926120 None:intergenic 30.0%
TTGGAGATTATTGAATCAAC+TGG - chr2.1:4926062-4926081 MS.gene057003:intron 30.0%
TTTAGGGATAAATCTGTTTC+TGG - chr2.1:4924349-4924368 MS.gene057003:intron 30.0%
! AAGTCAAGAAACTGATTTCA+AGG - chr2.1:4925425-4925444 MS.gene057003:intron 30.0%
! GATGTTTTCTGTCTATTCTT+GGG - chr2.1:4925945-4925964 MS.gene057003:intron 30.0%
! TGATGTTTTCTGTCTATTCT+TGG - chr2.1:4925944-4925963 MS.gene057003:intron 30.0%
! TTTGATTCATCTTGTTGTGA+AGG + chr2.1:4924033-4924052 None:intergenic 30.0%
!! CTATACTCTAGAAGCAATTT+TGG - chr2.1:4924948-4924967 MS.gene057003:CDS 30.0%
!! TTGAATCCATGTTTTGTTCA+GGG - chr2.1:4926005-4926024 MS.gene057003:intron 30.0%
AAAAAAAGAACGCACGATTG+AGG + chr2.1:4924228-4924247 None:intergenic 35.0%
AAATCAATTCTTGTTACCGC+AGG + chr2.1:4924707-4924726 None:intergenic 35.0%
AATTCGATAGGGTTAGGATT+AGG + chr2.1:4924087-4924106 None:intergenic 35.0%
ACTTCAGACTAGAGGTAAAT+AGG - chr2.1:4926123-4926142 MS.gene057003:intron 35.0%
AGAAATAGATGACGATGATC+TGG - chr2.1:4926486-4926505 MS.gene057003:intron 35.0%
ATATGAGGAGAAAGAGATAC+CGG + chr2.1:4926707-4926726 None:intergenic 35.0%
ATTCGATAGGGTTAGGATTA+GGG + chr2.1:4924086-4924105 None:intergenic 35.0%
CTGATAGCTGCATTTATCAA+CGG + chr2.1:4925471-4925490 None:intergenic 35.0%
CTGTTTGTGAGCAAGATAAT+GGG + chr2.1:4924123-4924142 None:intergenic 35.0%
GAAGAAATACTTCAGACTAG+AGG - chr2.1:4926115-4926134 MS.gene057003:intron 35.0%
GCAAAGAACAGTATAACTCA+TGG + chr2.1:4926327-4926346 None:intergenic 35.0%
GCACAATGCAGTAAAACATA+TGG + chr2.1:4925614-4925633 None:intergenic 35.0%
GGTTATTAGTGGGATTAATC+GGG - chr2.1:4925044-4925063 MS.gene057003:intron 35.0%
GTACAAACTCTGGTATTCTA+AGG - chr2.1:4925667-4925686 MS.gene057003:intron 35.0%
GTTATTAGTGGGATTAATCG+GGG - chr2.1:4925045-4925064 MS.gene057003:intron 35.0%
TGGATTCATCGAATTCGATA+GGG + chr2.1:4924098-4924117 None:intergenic 35.0%
TTCAAACGTCAACTTCTGAT+TGG - chr2.1:4926738-4926757 MS.gene057003:CDS 35.0%
TTGGATTCATCGAATTCGAT+AGG + chr2.1:4924099-4924118 None:intergenic 35.0%
! AGTGGTATCCTTTTATTGAC+AGG - chr2.1:4925732-4925751 MS.gene057003:intron 35.0%
! CTACTTGTACCTTCAGTTTT+TGG - chr2.1:4926450-4926469 MS.gene057003:intron 35.0%
! GTATGTGTGGTTTAACTTCT+GGG + chr2.1:4924978-4924997 None:intergenic 35.0%
! GTGGTATCCTTTTATTGACA+GGG - chr2.1:4925733-4925752 MS.gene057003:intron 35.0%
! GTTGATAAATGCAGCTATCA+GGG - chr2.1:4925470-4925489 MS.gene057003:CDS 35.0%
! TATGTGTGGTTTAACTTCTG+GGG + chr2.1:4924977-4924996 None:intergenic 35.0%
! TGTATGTGTGGTTTAACTTC+TGG + chr2.1:4924979-4924998 None:intergenic 35.0%
! TTTGAAACAGCTAACCAGTT+TGG + chr2.1:4925796-4925815 None:intergenic 35.0%
! TTTGAAGCCCTGTCAATAAA+AGG + chr2.1:4925743-4925762 None:intergenic 35.0%
!! AGTTGGTGATTCTATACATC+TGG - chr2.1:4925335-4925354 MS.gene057003:intron 35.0%
!! CTTGAATCCATGTTTTGTTC+AGG - chr2.1:4926004-4926023 MS.gene057003:intron 35.0%
!! TGTGTTTCATTGGCTTTATC+TGG - chr2.1:4924604-4924623 MS.gene057003:intron 35.0%
!! TTCTAAGGTGTTTGGCTAAT+TGG - chr2.1:4925682-4925701 MS.gene057003:CDS 35.0%
ACCTGAAATCTCAAAAGCAG+TGG + chr2.1:4924431-4924450 None:intergenic 40.0%
AGAAATGACCAGTTGGATAG+CGG + chr2.1:4925822-4925841 None:intergenic 40.0%
AGGATTAGGGTTATCATCGT+TGG + chr2.1:4924073-4924092 None:intergenic 40.0%
ATGAATCCGTCAGAATGAGA+AGG + chr2.1:4926651-4926670 None:intergenic 40.0%
ATTAGGGTTATCATCGTTGG+TGG + chr2.1:4924070-4924089 None:intergenic 40.0%
CAGCACTATTAGTGTATGTG+TGG + chr2.1:4924991-4925010 None:intergenic 40.0%
CGCAAACATGGACATTGTAA+AGG + chr2.1:4924203-4924222 None:intergenic 40.0%
CTATCCAACTGGTCATTTCT+TGG - chr2.1:4925822-4925841 MS.gene057003:intron 40.0%
CTTCTTGAAGATTGGAAACG+AGG + chr2.1:4923987-4924006 None:intergenic 40.0%
GCTGTTTGTGAGCAAGATAA+TGG + chr2.1:4924124-4924143 None:intergenic 40.0%
GGCTACGCAGAAAAAGAATT+TGG - chr2.1:4926043-4926062 MS.gene057003:intron 40.0%
TAATAATTCAGGGACTCCTG+CGG - chr2.1:4924579-4924598 MS.gene057003:intron 40.0%
TAATTGTGTGTTTGGTCCTG+CGG - chr2.1:4924688-4924707 MS.gene057003:CDS 40.0%
TCAATTGTACACTGATGCAG+TGG - chr2.1:4925714-4925733 MS.gene057003:intron 40.0%
TCAGACTAGAGGTAAATAGG+AGG - chr2.1:4926126-4926145 MS.gene057003:intron 40.0%
TCATCGAATTCGATAGGGTT+AGG + chr2.1:4924093-4924112 None:intergenic 40.0%
TGAATCCGTCAGAATGAGAA+GGG + chr2.1:4926650-4926669 None:intergenic 40.0%
TTACAGACCTTGTTGCTCAA+AGG + chr2.1:4925555-4925574 None:intergenic 40.0%
TTGTTGCTCAAAGGTGATCT+CGG + chr2.1:4925546-4925565 None:intergenic 40.0%
! ATCATCGTTGGTGGAAGAAA+CGG + chr2.1:4924061-4924080 None:intergenic 40.0%
! CAACTTGCTTTCACACATGA+AGG + chr2.1:4924802-4924821 None:intergenic 40.0%
! CGTTGATAAATGCAGCTATC+AGG - chr2.1:4925469-4925488 MS.gene057003:CDS 40.0%
! GATTACAGAGCACTTGAAAG+TGG - chr2.1:4926508-4926527 MS.gene057003:intron 40.0%
! TGCAAATGCTCTAGTCTTCT+AGG - chr2.1:4925631-4925650 MS.gene057003:intron 40.0%
!! CTCTGGTATTCTAAGGTGTT+TGG - chr2.1:4925674-4925693 MS.gene057003:intron 40.0%
!! GAGAAGTGCTTCTTGAAGAT+TGG + chr2.1:4923995-4924014 None:intergenic 40.0%
!! GCTGAAATGGTGTTGCTTTA+GGG - chr2.1:4924333-4924352 MS.gene057003:intron 40.0%
!! TGCTGAAATGGTGTTGCTTT+AGG - chr2.1:4924332-4924351 MS.gene057003:intron 40.0%
!! TGTGAGCAAGATAATGGGTT+TGG + chr2.1:4924118-4924137 None:intergenic 40.0%
!!! ACCTGGAGCATTTTAAAGCA+TGG + chr2.1:4925236-4925255 None:intergenic 40.0%
!!! TATCTGGAGCTTTGTTTTCG+TGG - chr2.1:4924620-4924639 MS.gene057003:intron 40.0%
AAATGCTCCAGGTTCTACTC+TGG - chr2.1:4925243-4925262 MS.gene057003:CDS 45.0%
AACAGCTAACCAGTTTGGAG+TGG + chr2.1:4925791-4925810 None:intergenic 45.0%
AATCCGTCAGAATGAGAAGG+GGG + chr2.1:4926648-4926667 None:intergenic 45.0%
AATGCTCCAGGTTCTACTCT+GGG - chr2.1:4925244-4925263 MS.gene057003:CDS 45.0%
ACGATTGAGGAACGCAAACA+TGG + chr2.1:4924215-4924234 None:intergenic 45.0%
ACGGATTCATGCATGTGCTT+TGG - chr2.1:4926661-4926680 MS.gene057003:CDS 45.0%
AGCTATCAGGGATGCTGAAA+AGG - chr2.1:4925482-4925501 MS.gene057003:CDS 45.0%
ATCTTGCTCACAAACAGCGA+TGG - chr2.1:4924126-4924145 MS.gene057003:intron 45.0%
ATTAATCGGGGATCAAGCTG+CGG - chr2.1:4925057-4925076 MS.gene057003:intron 45.0%
ATTGAATCAACTGGAGCTGC+AGG - chr2.1:4926071-4926090 MS.gene057003:intron 45.0%
ATTTCTGCTGTGATTCCTGG+TGG - chr2.1:4926761-4926780 MS.gene057003:CDS 45.0%
CATTTCTTGGCCAATCCACA+AGG - chr2.1:4925835-4925854 MS.gene057003:intron 45.0%
GAAGAACGAGAGCTTCAACT+AGG + chr2.1:4924176-4924195 None:intergenic 45.0%
GAATCCGTCAGAATGAGAAG+GGG + chr2.1:4926649-4926668 None:intergenic 45.0%
GAATGAGAAGGGGGGAAAAT+TGG + chr2.1:4926639-4926658 None:intergenic 45.0%
GACTAGAGGTAAATAGGAGG+TGG - chr2.1:4926129-4926148 MS.gene057003:intron 45.0%
GAGATCACCTTTGAGCAACA+AGG - chr2.1:4925545-4925564 MS.gene057003:intron 45.0%
GCTGCTTGTCCAAAAACTGA+AGG + chr2.1:4926462-4926481 None:intergenic 45.0%
GCTTGTTCCGCTCAAGTTAA+CGG - chr2.1:4924403-4924422 MS.gene057003:intron 45.0%
TAGAGGTAAATAGGAGGTGG+AGG - chr2.1:4926132-4926151 MS.gene057003:intron 45.0%
TAGGATACTTACAGCTGCAG+AGG + chr2.1:4925899-4925918 None:intergenic 45.0%
TCAAATAGCCGCTATCCAAC+TGG - chr2.1:4925811-4925830 MS.gene057003:intron 45.0%
TCAACTAGGTGTGTAAGACC+TGG + chr2.1:4924162-4924181 None:intergenic 45.0%
TGGATTTCTGCTGTGATTCC+TGG - chr2.1:4926758-4926777 MS.gene057003:CDS 45.0%
TGTTACGATTCGAGAAACCG+TGG - chr2.1:4924378-4924397 MS.gene057003:intron 45.0%
TTGGCCAAGAAATGACCAGT+TGG + chr2.1:4925829-4925848 None:intergenic 45.0%
! TCCTGCGGATTGTGTTTCAT+TGG - chr2.1:4924594-4924613 MS.gene057003:intron 45.0%
! TTCGTGGTCAAAGCCTGTTT+TGG - chr2.1:4924636-4924655 MS.gene057003:CDS 45.0%
!! GCCATGCTTTAAAATGCTCC+AGG - chr2.1:4925232-4925251 MS.gene057003:CDS 45.0%
!! GCTAGGGAGGCATTATTATG+CGG - chr2.1:4925279-4925298 MS.gene057003:CDS 45.0%
!! TCAGTTTTTGGACAAGCAGC+AGG - chr2.1:4926462-4926481 MS.gene057003:intron 45.0%
!! TGCATGTGCTTTGGAGAAAC+AGG - chr2.1:4926670-4926689 MS.gene057003:CDS 45.0%
!!! GCCACTGCTTTTGAGATTTC+AGG - chr2.1:4924427-4924446 MS.gene057003:intron 45.0%
AACAACCCCAGAGTAGAACC+TGG + chr2.1:4925253-4925272 None:intergenic 50.0%
AGCGATGGAGTTGAATCACC+AGG - chr2.1:4924141-4924160 MS.gene057003:intron 50.0%
AGGTAAATAGGAGGTGGAGG+AGG - chr2.1:4926135-4926154 MS.gene057003:intron 50.0%
ATCCGTCAGAATGAGAAGGG+GGG + chr2.1:4926647-4926666 None:intergenic 50.0%
ATGCTCCAGGTTCTACTCTG+GGG - chr2.1:4925245-4925264 MS.gene057003:CDS 50.0%
GAAACAGGTTGCAGTGACTC+CGG - chr2.1:4926685-4926704 MS.gene057003:CDS 50.0%
GCCAATGAAACACAATCCGC+AGG + chr2.1:4924598-4924617 None:intergenic 50.0%
GCTGTGCAAACTGAAGACCA+AGG + chr2.1:4925864-4925883 None:intergenic 50.0%
GGTCTTCAGTTTGCACAGCT+TGG - chr2.1:4925865-4925884 MS.gene057003:intron 50.0%
TAAATAGGAGGTGGAGGAGG+AGG - chr2.1:4926138-4926157 MS.gene057003:intron 50.0%
TGAAGATTGGAAACGAGGCC+CGG + chr2.1:4923982-4924001 None:intergenic 50.0%
TGTGGAGATCCACTCCAAAC+TGG - chr2.1:4925779-4925798 MS.gene057003:intron 50.0%
TTCCCCCCTTCTCATTCTGA+CGG - chr2.1:4926642-4926661 MS.gene057003:CDS 50.0%
TTCTTGGCCAATCCACAAGG+TGG - chr2.1:4925838-4925857 MS.gene057003:intron 50.0%
TTGAGCGGAACAAGCTTCCA+CGG + chr2.1:4924398-4924417 None:intergenic 50.0%
! AGTGGCACCGTTAACTTGAG+CGG + chr2.1:4924413-4924432 None:intergenic 50.0%
!! TTACAGCTGCAGAGGCATCT+CGG + chr2.1:4925891-4925910 None:intergenic 50.0%
!! TTCTACTCTGGGGTTGTTGC+TGG - chr2.1:4925255-4925274 MS.gene057003:CDS 50.0%
AAGATTGGAAACGAGGCCCG+GGG + chr2.1:4923980-4923999 None:intergenic 55.0%
ACCAAGGCCACCTTGTGGAT+TGG + chr2.1:4925848-4925867 None:intergenic 55.0%
GAAGATTGGAAACGAGGCCC+GGG + chr2.1:4923981-4924000 None:intergenic 55.0%
GCTGCGCCCTGAACAAAACA+TGG + chr2.1:4926014-4926033 None:intergenic 55.0%
TGAAGACCAAGGCCACCTTG+TGG + chr2.1:4925853-4925872 None:intergenic 55.0%
TGGGGTTGTTGCTGGAGCTA+GGG - chr2.1:4925263-4925282 MS.gene057003:CDS 55.0%
AGATTGGAAACGAGGCCCGG+GGG + chr2.1:4923979-4923998 None:intergenic 60.0%
CTGGGGTTGTTGCTGGAGCT+AGG - chr2.1:4925262-4925281 MS.gene057003:CDS 60.0%
GCCAATCCACAAGGTGGCCT+TGG - chr2.1:4925844-4925863 MS.gene057003:intron 60.0%
GGTTGTTGCTGGAGCTAGGG+AGG - chr2.1:4925266-4925285 MS.gene057003:CDS 60.0%
GTGGAGGAGGAGGATGAGGA+GGG - chr2.1:4926148-4926167 MS.gene057003:intron 60.0%
TGGAGGAGGAGGATGAGGAG+GGG - chr2.1:4926149-4926168 MS.gene057003:intron 60.0%
TTGTTCAGGGCGCAGCTCGT+CGG - chr2.1:4926018-4926037 MS.gene057003:intron 60.0%
GGAGGAGGAGGATGAGGAGG+GGG - chr2.1:4926150-4926169 MS.gene057003:intron 65.0%
GGAGGTGGAGGAGGAGGATG+AGG - chr2.1:4926144-4926163 MS.gene057003:intron 65.0%
GGTGGAGGAGGAGGATGAGG+AGG - chr2.1:4926147-4926166 MS.gene057003:intron 65.0%
TCAGGGCGCAGCTCGTCGGT+TGG - chr2.1:4926022-4926041 MS.gene057003:intron 70.0%


Chromosome Type Strat End Strand Name
chr2.1 gene 4923973 4926790 4923973 ID=MS.gene057003
chr2.1 mRNA 4923973 4926790 4923973 ID=MS.gene057003.t1;Parent=MS.gene057003
chr2.1 exon 4926601 4926790 4926601 ID=MS.gene057003.t1.exon1;Parent=MS.gene057003.t1
chr2.1 CDS 4926601 4926790 4926601 ID=cds.MS.gene057003.t1;Parent=MS.gene057003.t1
chr2.1 exon 4926315 4926409 4926315 ID=MS.gene057003.t1.exon2;Parent=MS.gene057003.t1
chr2.1 CDS 4926315 4926409 4926315 ID=cds.MS.gene057003.t1;Parent=MS.gene057003.t1
chr2.1 exon 4925675 4925718 4925675 ID=MS.gene057003.t1.exon3;Parent=MS.gene057003.t1
chr2.1 CDS 4925675 4925718 4925675 ID=cds.MS.gene057003.t1;Parent=MS.gene057003.t1
chr2.1 exon 4925432 4925509 4925432 ID=MS.gene057003.t1.exon4;Parent=MS.gene057003.t1
chr2.1 CDS 4925432 4925509 4925432 ID=cds.MS.gene057003.t1;Parent=MS.gene057003.t1
chr2.1 exon 4925197 4925350 4925197 ID=MS.gene057003.t1.exon5;Parent=MS.gene057003.t1
chr2.1 CDS 4925197 4925350 4925197 ID=cds.MS.gene057003.t1;Parent=MS.gene057003.t1
chr2.1 exon 4924857 4925009 4924857 ID=MS.gene057003.t1.exon6;Parent=MS.gene057003.t1
chr2.1 CDS 4924857 4925009 4924857 ID=cds.MS.gene057003.t1;Parent=MS.gene057003.t1
chr2.1 exon 4924627 4924737 4924627 ID=MS.gene057003.t1.exon7;Parent=MS.gene057003.t1
chr2.1 CDS 4924627 4924737 4924627 ID=cds.MS.gene057003.t1;Parent=MS.gene057003.t1
chr2.1 exon 4924229 4924297 4924229 ID=MS.gene057003.t1.exon8;Parent=MS.gene057003.t1
chr2.1 CDS 4924229 4924297 4924229 ID=cds.MS.gene057003.t1;Parent=MS.gene057003.t1
chr2.1 exon 4923973 4924071 4923973 ID=MS.gene057003.t1.exon9;Parent=MS.gene057003.t1
chr2.1 CDS 4923973 4924071 4923973 ID=cds.MS.gene057003.t1;Parent=MS.gene057003.t1
Gene Sequence

>MS.gene057003

ATGCCCCCGGGCCTCGTTTCCAATCTTCAAGAAGCACTTCTCAACAGAAAAATCACACCTTCACAACAAGATGAATCAAACGACACCGTTTCTTCCACCAACGATGATAACCCTAATCCTAACCCTATCGAATTCGATGAATCCAAACCCATTATCTTGCTCACAAACAGCGATGGAGTTGAATCACCAGGTCTTACACACCTAGTTGAAGCTCTCGTTCTTCAAAACCTTTACAATGTCCATGTTTGCGTTCCTCAATCGTGCGTTCTTTTTTTTTTAATCTGTTAAGAAATGAAGTAAATTTTTGATTTTTTTGTTGGTATTGAATAATGTTGAATGTGTTTGTAATTTTTGTTAGTTGCTGAAATGGTGTTGCTTTAGGGATAAATCTGTTTCTGGTCATTCTGTTACGATTCGAGAAACCGTGGAAGCTTGTTCCGCTCAAGTTAACGGTGCCACTGCTTTTGAGATTTCAGGTATTAACTTCATTCAATTCTGTTTCTAAATCAAATTTTTGTTTTTTGGGGGTGTGCTTATGAATTTTTTGATATTAATAATGTAATATAGTTATATGAATTTGTTAACGTTTTCTAATTTAGTAATTTATAATAATTCAGGGACTCCTGCGGATTGTGTTTCATTGGCTTTATCTGGAGCTTTGTTTTCGTGGTCAAAGCCTGTTTTGGTTAGCATTTATTATCTTACACCCGTAAATTAATTGTGTGTTTGGTCCTGCGGTAACAAGAATTGATTTTGAATAAATTGATTATGTAAAATTGATTCTGGCTTTAAGTGAGTTGAAAGTAAAATTATTTATGTTTGAATACCTTCATGTGTGAAAGCAAGTTGAACGATAAATTTGAGACTAGAAATCAGTTCTAGAATCAAAAGCTCCAAATTCTAGCTTCAAGTTAGAATAATTTTGAAGGCAGAATCAATTATACTCGAGAGCAATCAAACATGTCAAAATCAATTCTATACTCTAGAAGCAATTTTGGCTACCCCAGAAGTTAAACCACACATACACTAATAGTGCTGTGAAATAGTAATCATGATGTGATTTGAATTTCAGGTTATTAGTGGGATTAATCGGGGATCAAGCTGCGGTCATCACATGTAAGTTGCTAACTTATGGTTAGGGAGAAGTTAAACACATTTTCCATTTTCTGTCTTGTAATATTCTATATGTATTGTTTTTGTTTTAAGATTTGTATCTATTGTCCATAATTTGCAGCCTTGTTATAGAGATTTGTGTTTATGCCATGCTTTAAAATGCTCCAGGTTCTACTCTGGGGTTGTTGCTGGAGCTAGGGAGGCATTATTATGCGGTGTACCATCATTATCAATATCATTAAACTGGTAAGTTGGTGATTCTATACATCTGGTTTTAACTATGCTTTCATTAGAGATTATGAATTGGTATCATATATCATGTTATAGGAAAAAGGATGAAAGTCAAGAAACTGATTTCAAGGATGCAGTTGAAGTCTGTTTACCGTTGATAAATGCAGCTATCAGGGATGCTGAAAAGGAAACATTTCCCAAAAATTGCTTTCTGAATATTGAAATTCCGAGATCACCTTTGAGCAACAAGGTCTGTAAATTTTGTTATATAATAATTTGATTGTTTACTTTTTCCCATATGTTTTACTGCATTGTGCAAATGCTCTAGTCTTCTAGGTTTTCTTTCAATTGTACAAACTCTGGTATTCTAAGGTGTTTGGCTAATTGGTTCTAATGATCAATTGTACACTGATGCAGTGGTATCCTTTTATTGACAGGGCTTCAAATTGACTAAGCAAAGTATGTGGAGATCCACTCCAAACTGGTTAGCTGTTTCAAATAGCCGCTATCCAACTGGTCATTTCTTGGCCAATCCACAAGGTGGCCTTGGTCTTCAGTTTGCACAGCTTGGCCGAGATGCCTCTGCAGCTGTAAGTATCCTACATTACATACAAACATGTGTTATGTTGATGTTTTCTGTCTATTCTTGGGTGTGATTTTAATGTGTTTAATCTATTGATGCTGATACTTGAATCCATGTTTTGTTCAGGGCGCAGCTCGTCGGTTGGCTACGCAGAAAAAGAATTTGGAGATTATTGAATCAACTGGAGCTGCAGGAAAACCTGATACCAATAAAGTGAAGAAATACTTCAGACTAGAGGTAAATAGGAGGTGGAGGAGGAGGATGAGGAGGGGGATCTTGATTAGAATAGAATTTGCTTTGTACAAATCTTGTTAATGTTGAAGTTAGTCTGTTTTCAATGCTGAGTACAGAAGATAACAATTTGTGGAGAATGGATTATCATAGAAGTTATCTTCATACTTGAGAAGATTTATTTATGTTTTAACCATGAGTTATACTGTTCTTTGCCTTTTAGATTATTAATCTGTTCCCTTCATACAACATTATGTGTTATATTTGTATTTCAATTAAGTAACATCAAATTATTTGTACAAGTGAACTTAGCTATAATCTACTTGTACCTTCAGTTTTTGGACAAGCAGCAGGAAGAAATAGATGACGATGATCTGGATTACAGAGCACTTGAAAGTGGATATGTAAGTTTGAGCAATAAAATATTTTTTCAATTGATATTAGTTATCTTTAACTATATTGTATGTTCTTGATGGTAATATCTTTATGGGTTGTTATACATGCACCAATTTTCCCCCCTTCTCATTCTGACGGATTCATGCATGTGCTTTGGAGAAACAGGTTGCAGTGACTCCGGTATCTCTTTCTCCTCATATTGAGAGTGATATTCAAACGTCAACTTCTGATTGGATTTCTGCTGTGATTCCTGGTGGACAGTAA
Protein sequence

>MS.gene057003.t1

MPPGLVSNLQEALLNRKITPSQQDESNDTVSSTNDDNPNPNPIEFDESKPIILLTNSDGVESPGINLFLVILLRFEKPWKLVPLKLTVPLLLRFQVISGINRGSSCGHHMFYSGVVAGAREALLCGVPSLSISLNWKKDESQETDFKDAVEVCLPLINAAIRDAEKETFPKNCFLNIEIPRSPLSNKGFKLTKQSMWRSTPNWLAVSNSRYPTGHFLANPQGGLGLQFAQLGRDASAAGAARRLATQKKNLEIIESTGAAGKPDTNKVKKYFRLEFLDKQQEEIDDDDLDYRALESGYVAVTPVSLSPHIESDIQTSTSDWISAVIPGGQ