AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene22522

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene22522.t1 MTR_2g040830 85.241 332 43 4 1 329 1 329 0.0 557
MS.gene22522.t1 MTR_2g040690 82.335 334 51 5 1 329 1 331 0.0 536
MS.gene22522.t1 MTR_2g040740 80.723 332 57 6 1 329 1 328 0.0 511
MS.gene22522.t1 MTR_2g040650 81.818 330 54 5 1 329 1 325 1.09e-180 502
MS.gene22522.t1 MTR_1g099310 51.803 305 133 8 1 300 1 296 8.02e-97 288
MS.gene22522.t1 MTR_5g043550 52.508 299 126 8 10 300 4 294 1.74e-96 288
MS.gene22522.t1 MTR_0027s0260 48.684 304 141 8 5 300 2 298 5.40e-93 279
MS.gene22522.t1 MTR_8g055940 47.386 306 146 8 1 300 1 297 4.87e-91 274
MS.gene22522.t1 MTR_1g099290 49.315 292 137 6 11 297 10 295 3.86e-88 275
MS.gene22522.t1 MTR_1g099320 51.661 271 120 7 23 287 20 285 5.07e-87 263
MS.gene22522.t1 MTR_2g040880 85.034 147 21 1 183 329 1 146 1.62e-86 257
MS.gene22522.t1 MTR_7g116850 47.138 297 145 8 11 300 35 326 2.99e-80 247
MS.gene22522.t1 MTR_7g116350 45.455 297 138 7 11 302 9 286 4.90e-79 257
MS.gene22522.t1 MTR_8g467650 47.176 301 143 10 8 300 6 298 8.15e-79 243
MS.gene22522.t1 MTR_2g102020 46.075 293 140 8 7 286 13 300 2.12e-77 241
MS.gene22522.t1 MTR_1g099350 47.388 268 128 7 24 286 19 278 2.67e-77 238
MS.gene22522.t1 MTR_6g023340 45.105 286 142 9 24 300 23 302 2.72e-74 231
MS.gene22522.t1 MTR_2g040870 70.253 158 43 2 17 171 13 169 3.96e-72 221
MS.gene22522.t1 MTR_2g040900 59.355 155 61 2 176 329 17 170 6.99e-58 184
MS.gene22522.t1 MTR_2g040890 55.367 177 70 5 1 171 1 174 2.00e-56 182
MS.gene22522.t1 MTR_2g040850 53.595 153 57 4 1 142 1 150 2.35e-42 147
MS.gene22522.t1 MTR_2g040850 50.000 126 54 4 198 320 122 241 7.18e-26 103
MS.gene22522.t1 MTR_4g077310 43.158 95 48 3 204 298 3 91 1.97e-16 73.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene22522.t1 AT5G24090 46.535 303 152 7 3 300 5 302 8.51e-90 271

Find 69 sgRNAs with CRISPR-Local

Find 200 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGGCTTTCCTCAATGTCTT+TGG 0.217357 2.2:+47308865 MS.gene22522:CDS
ATAAAACACTGTCAAGAAAA+AGG 0.225882 2.2:+47308967 MS.gene22522:CDS
CCCATGAAAACGGTATTATT+TGG 0.251275 2.2:-47311447 None:intergenic
AACTTTCTTAGTGGTCAATT+TGG 0.254606 2.2:+47309075 MS.gene22522:CDS
ATAGTTGGAAGTTGGTTTAA+TGG 0.284816 2.2:-47311547 None:intergenic
TTACTTTCCCTCAGAGCATT+AGG 0.302991 2.2:+47309000 MS.gene22522:CDS
AAGGAGGTACAAATCTTTAC+TGG 0.304361 2.2:+47309142 MS.gene22522:CDS
ATTAAACCAACTTCCAACTA+TGG 0.306183 2.2:+47311549 MS.gene22522:CDS
GTTAAAATCAACACCATCTA+AGG 0.310593 2.2:-47309115 None:intergenic
ACCAGATGATCTCATTTCTA+AGG 0.326123 2.2:+47311515 MS.gene22522:CDS
ATGGTGGCATTGCCGTCTAC+TGG 0.329897 2.2:+47308776 MS.gene22522:CDS
CTTGGAAGTGTTACCTTAGA+TGG 0.333989 2.2:+47309102 MS.gene22522:CDS
CTCTATACTAACTTTCTTAG+TGG 0.345832 2.2:+47309066 MS.gene22522:CDS
AGCACAAAATGGATACAAAA+AGG 0.354708 2.2:+47308689 None:intergenic
TAATTTCGAGATTGTACTCT+TGG 0.374143 2.2:+47308846 MS.gene22522:CDS
CTTGACAAAGCTATCAAAAC+TGG 0.380969 2.2:+47311306 MS.gene22522:CDS
CTATGGAGGAATTATGCTTT+GGG 0.391332 2.2:+47311566 MS.gene22522:CDS
GTAATCTGGCATATAACATT+GGG 0.392231 2.2:-47311280 None:intergenic
ACTATGGAGGAATTATGCTT+TGG 0.402308 2.2:+47311565 MS.gene22522:CDS
TGGTGGCATTGCCGTCTACT+GGG 0.405455 2.2:+47308777 MS.gene22522:CDS
GCTTTCCTCAATGTCTTTGG+AGG 0.413044 2.2:+47308868 MS.gene22522:CDS
TGATTTGCAAAGTTCAAACT+TGG 0.417925 2.2:-47308901 None:intergenic
TAGCTTCTTCTGGTGAGCTA+AGG 0.426254 2.2:-47309026 None:intergenic
TGATTACAGCTATCAGATAA+AGG 0.427044 2.2:+47311614 MS.gene22522:CDS
CTACATTGCTCTTAAAATCA+TGG 0.429928 2.2:+47311400 MS.gene22522:CDS
ACCTTAGAAATGAGATCATC+TGG 0.432094 2.2:-47311516 None:intergenic
TTCCTCAATGTCTTTGGAGG+AGG 0.446588 2.2:+47308871 MS.gene22522:CDS
AGCTTCTTCTGGTGAGCTAA+GGG 0.448193 2.2:-47309025 None:intergenic
TGGGATAGATTTCATGATGT+TGG 0.451538 2.2:+47311585 MS.gene22522:CDS
CAAGTTTCTGAGGCAATAGC+TGG 0.454724 2.2:+47311672 MS.gene22522:CDS
ACAATCTAAGACAACAAAAC+AGG 0.458920 2.2:+47309187 MS.gene22522:CDS
AAGCATAATTCCTCCATAGT+TGG 0.460777 2.2:-47311562 None:intergenic
TCTTAAAATCATGGAATGCT+TGG 0.464778 2.2:+47311409 MS.gene22522:CDS
CCACCACTAGGAGCTGCATT+AGG 0.480850 2.2:-47311483 None:intergenic
ATTCCTCCATAGTTGGAAGT+TGG 0.484513 2.2:-47311555 None:intergenic
TTTGTTGTCTTAGATTGTCA+AGG 0.485852 2.2:-47309182 None:intergenic
GCTTTGTCAAGGTAGTAATC+TGG 0.487955 2.2:-47311294 None:intergenic
TTTACTGGGATGACCTTGCT+AGG 0.501225 2.2:+47309157 MS.gene22522:CDS
TTGGAAGTTGGTTTAATGGA+AGG 0.522478 2.2:-47311543 None:intergenic
AGTAATCTGGCATATAACAT+TGG 0.524590 2.2:-47311281 None:intergenic
GGAGCTGCATTAGGTGCTGC+TGG 0.526335 2.2:-47311474 None:intergenic
TGCTGGTAGTCCCATGAAAA+CGG 0.527292 2.2:-47311457 None:intergenic
CACTAAGAAAGTTAGTATAG+AGG 0.529347 2.2:-47309065 None:intergenic
GATTTGCAAAGTTCAAACTT+GGG 0.564533 2.2:-47308900 None:intergenic
AGTGGTCAATTTGGTCCACT+TGG 0.566066 2.2:+47309084 MS.gene22522:CDS
AAGGGCTCCTAATGCTCTGA+GGG 0.572834 2.2:-47309007 None:intergenic
TATGATTTAATTCGTAAACA+TGG 0.576145 2.2:-47311718 None:intergenic
TTGAACTTTGCAAATCATTG+TGG 0.576615 2.2:+47308907 MS.gene22522:CDS
TTCCTCCTCCAAAGACATTG+AGG 0.584718 2.2:-47308873 None:intergenic
TGATGATGCTTTGATAGTGA+AGG 0.585896 2.2:-47308749 None:intergenic
TTACTGGGATGACCTTGCTA+GGG 0.587273 2.2:+47309158 MS.gene22522:CDS
AGGAGGTACAAATCTTTACT+GGG 0.587554 2.2:+47309143 MS.gene22522:CDS
TGGGGCCAAAACGTAAAAGA+AGG 0.591748 2.2:+47308796 MS.gene22522:CDS
GATTGTCAAGGTCCCTAGCA+AGG 0.595500 2.2:-47309170 None:intergenic
ATATTGTCAAGACTAACAAA+AGG 0.600315 2.2:-47308723 None:intergenic
ATCAAAGCATCATCAAGTGA+TGG 0.610130 2.2:+47308757 MS.gene22522:CDS
TTGACATCAACATGTGACAC+TGG 0.615620 2.2:+47308823 MS.gene22522:CDS
GATGATGCTTTGATAGTGAA+GGG 0.636457 2.2:-47308748 None:intergenic
AAACCAACTTCCAACTATGG+AGG 0.642148 2.2:+47311552 MS.gene22522:CDS
AAAGCATCATCAAGTGATGG+TGG 0.646449 2.2:+47308760 MS.gene22522:CDS
TAAGGGCTCCTAATGCTCTG+AGG 0.652053 2.2:-47309008 None:intergenic
GGTGGCATTGCCGTCTACTG+GGG 0.654303 2.2:+47308778 MS.gene22522:CDS
GCACCTAATGCAGCTCCTAG+TGG 0.656531 2.2:+47311480 MS.gene22522:CDS
AGTGTCACATGTTGATGTCA+AGG 0.656681 2.2:-47308821 None:intergenic
CCTAATGCAGCTCCTAGTGG+TGG 0.665138 2.2:+47311483 MS.gene22522:CDS
TAATCTGGCATATAACATTG+GGG 0.697221 2.2:-47311279 None:intergenic
GGTGGTATATAACCACCACT+AGG 0.701616 2.2:-47311495 None:intergenic
TCTAAGGTAACACTTCCAAG+TGG 0.714737 2.2:-47309099 None:intergenic
TTAGAAATGAGATCATCTGG+TGG 0.728954 2.2:-47311513 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATATGAGAAAATATAATAT+AGG + chr2.2:47309775-47309794 MS.gene22522:intron 10.0%
!! TAATAAATATTTACCATTTA+TGG + chr2.2:47310890-47310909 MS.gene22522:intron 10.0%
!!! TATTTTTCTTTGAAAAAATA+TGG - chr2.2:47309668-47309687 None:intergenic 10.0%
!! ATACTTATGTTACAAATATT+TGG - chr2.2:47310344-47310363 None:intergenic 15.0%
!! TACTTATGTTACAAATATTT+GGG - chr2.2:47310343-47310362 None:intergenic 15.0%
!!! GAAATGATGTTTTTTTTTAT+GGG - chr2.2:47311095-47311114 None:intergenic 15.0%
!!! TATCCATTCTAAAATTATTA+TGG - chr2.2:47310099-47310118 None:intergenic 15.0%
!!! TGAAATGATGTTTTTTTTTA+TGG - chr2.2:47311096-47311115 None:intergenic 15.0%
!!! TTAATTTATTGATTTATGAC+AGG - chr2.2:47311013-47311032 None:intergenic 15.0%
!!! TTTTCAAAGAAAAATATGTA+TGG + chr2.2:47309672-47309691 MS.gene22522:intron 15.0%
!!! TTTTTTTTATCATATTGACA+TGG - chr2.2:47311372-47311391 None:intergenic 15.0%
!! AAAAAAAAAAAATCTCTTGC+AGG + chr2.2:47311235-47311254 MS.gene22522:intron 20.0%
!! AAAAAATATGGTTGTTCATT+AGG - chr2.2:47309656-47309675 None:intergenic 20.0%
!! AAAGAAATATATCATGACAT+GGG - chr2.2:47309511-47309530 None:intergenic 20.0%
!! AAATATAATGTGAGTATAAG+AGG + chr2.2:47309723-47309742 MS.gene22522:intron 20.0%
!! AGCATAATGCTATAATATAT+TGG + chr2.2:47310945-47310964 MS.gene22522:intron 20.0%
!! AGTATTATATGAACTAGTAT+TGG + chr2.2:47310169-47310188 MS.gene22522:intron 20.0%
!! ATATTCACATTATACACTTT+AGG - chr2.2:47309760-47309779 None:intergenic 20.0%
!! ATCTTTAAATTAAAGTGTAC+CGG + chr2.2:47310983-47311002 MS.gene22522:intron 20.0%
!! GCATAATGCTATAATATATT+GGG + chr2.2:47310946-47310965 MS.gene22522:intron 20.0%
!! TAAAGAAATATATCATGACA+TGG - chr2.2:47309512-47309531 None:intergenic 20.0%
!! TAGAATGGATATAGTAAAAA+AGG + chr2.2:47310108-47310127 MS.gene22522:intron 20.0%
!!! AAAAGGTTGATTTGTTAATT+AGG - chr2.2:47311193-47311212 None:intergenic 20.0%
!!! AAAGGTTGATTTGTTAATTA+GGG - chr2.2:47311192-47311211 None:intergenic 20.0%
!!! ACGGAAAATACTTTTTTTTT+AGG - chr2.2:47310379-47310398 None:intergenic 20.0%
!!! ACTTTTTTTTTAGGGAAATA+CGG - chr2.2:47310370-47310389 None:intergenic 20.0%
!!! AGATTTTTTTTTTTTGAGAG+AGG - chr2.2:47311230-47311249 None:intergenic 20.0%
!!! CGGAAAATACTTTTTTTTTA+GGG - chr2.2:47310378-47310397 None:intergenic 20.0%
!!! CTAGTACTTTTTTTTTGAAT+CGG - chr2.2:47309264-47309283 None:intergenic 20.0%
!!! TAGTACTTTTTTTTTGAATC+GGG - chr2.2:47309263-47309282 None:intergenic 20.0%
!!! TGCTTACTTTTATTTATGTT+TGG - chr2.2:47309936-47309955 None:intergenic 20.0%
!!! TTTAACTTAAGAAAGTAGAA+TGG + chr2.2:47310535-47310554 MS.gene22522:intron 20.0%
!!! TTTTTTATAAAATTGGGATG+TGG - chr2.2:47309393-47309412 None:intergenic 20.0%
! AAGAAATATATCATGACATG+GGG - chr2.2:47309510-47309529 None:intergenic 25.0%
! ATAAAACACTGTCAAGAAAA+AGG + chr2.2:47308967-47308986 MS.gene22522:CDS 25.0%
! ATATTGTCAAGACTAACAAA+AGG - chr2.2:47308726-47308745 None:intergenic 25.0%
! TAAAACACTGTCAAGAAAAA+GGG + chr2.2:47308968-47308987 MS.gene22522:CDS 25.0%
! TAATATATTGGGAGTTTCTA+CGG + chr2.2:47310957-47310976 MS.gene22522:intron 25.0%
! TGCAAATAAACGGATATAAT+TGG + chr2.2:47310709-47310728 MS.gene22522:intron 25.0%
! TTAGGATGTTAAATAAAACG+TGG - chr2.2:47309638-47309657 None:intergenic 25.0%
!! CAAAAATTTTCACTTGTCTT+CGG - chr2.2:47310578-47310597 None:intergenic 25.0%
!! GTTGAAATTTTACAATCTCA+GGG + chr2.2:47310731-47310750 MS.gene22522:intron 25.0%
!! TTATATGAACTAGTATTGGA+TGG + chr2.2:47310173-47310192 MS.gene22522:intron 25.0%
!! TTGAAATTTTACAATCTCAG+GGG + chr2.2:47310732-47310751 MS.gene22522:intron 25.0%
!! TTTTATCATATTGACATGGA+GGG - chr2.2:47311368-47311387 None:intergenic 25.0%
!! TTTTTATCATATTGACATGG+AGG - chr2.2:47311369-47311388 None:intergenic 25.0%
!!! GCTCCATAATAATTTTAGAA+TGG + chr2.2:47310093-47310112 MS.gene22522:intron 25.0%
!!! TTCTTGACAGTGTTTTATTT+CGG - chr2.2:47308965-47308984 None:intergenic 25.0%
AACTTTCTTAGTGGTCAATT+TGG + chr2.2:47309075-47309094 MS.gene22522:CDS 30.0%
AGTAATCTGGCATATAACAT+TGG - chr2.2:47311284-47311303 None:intergenic 30.0%
ATTAAACCAACTTCCAACTA+TGG + chr2.2:47311549-47311568 MS.gene22522:CDS 30.0%
ATTGGATGGACACAATATAA+GGG + chr2.2:47310187-47310206 MS.gene22522:intron 30.0%
CACTAAGAAAGTTAGTATAG+AGG - chr2.2:47309068-47309087 None:intergenic 30.0%
CACTTCAAATTAAGTTCTCA+GGG - chr2.2:47310476-47310495 None:intergenic 30.0%
CATGTTAAGGATTCCATAAA+TGG - chr2.2:47310906-47310925 None:intergenic 30.0%
CCCAGTTTGCATATTTAATA+CGG - chr2.2:47310398-47310417 None:intergenic 30.0%
CCGTATTAAATATGCAAACT+GGG + chr2.2:47310395-47310414 MS.gene22522:intron 30.0%
CTACATTGCTCTTAAAATCA+TGG + chr2.2:47311400-47311419 MS.gene22522:CDS 30.0%
CTCTATACTAACTTTCTTAG+TGG + chr2.2:47309066-47309085 MS.gene22522:CDS 30.0%
GATTTGCAAAGTTCAAACTT+GGG - chr2.2:47308903-47308922 None:intergenic 30.0%
GGTGCAAAATAAATTTCTCA+GGG + chr2.2:47310678-47310697 MS.gene22522:intron 30.0%
GTAATCTGGCATATAACATT+GGG - chr2.2:47311283-47311302 None:intergenic 30.0%
GTTAAAATCAACACCATCTA+AGG - chr2.2:47309118-47309137 None:intergenic 30.0%
TAATCTGGCATATAACATTG+GGG - chr2.2:47311282-47311301 None:intergenic 30.0%
TAATTTCGAGATTGTACTCT+TGG + chr2.2:47308846-47308865 MS.gene22522:CDS 30.0%
TATGCTAACTTGAGTTCTTA+AGG - chr2.2:47310931-47310950 None:intergenic 30.0%
TATTGGATGGACACAATATA+AGG + chr2.2:47310186-47310205 MS.gene22522:intron 30.0%
TCACTTCAAATTAAGTTCTC+AGG - chr2.2:47310477-47310496 None:intergenic 30.0%
TCCGTATTAAATATGCAAAC+TGG + chr2.2:47310394-47310413 MS.gene22522:intron 30.0%
TCTTAAAATCATGGAATGCT+TGG + chr2.2:47311409-47311428 MS.gene22522:CDS 30.0%
TGATTACAGCTATCAGATAA+AGG + chr2.2:47311614-47311633 MS.gene22522:CDS 30.0%
TGGTGCAAAATAAATTTCTC+AGG + chr2.2:47310677-47310696 MS.gene22522:intron 30.0%
TTGAACTTTGCAAATCATTG+TGG + chr2.2:47308907-47308926 MS.gene22522:CDS 30.0%
! ACAATCTAAGACAACAAAAC+AGG + chr2.2:47309187-47309206 MS.gene22522:CDS 30.0%
! ACCAAATAATACCGTTTTCA+TGG + chr2.2:47311446-47311465 MS.gene22522:CDS 30.0%
! ATAGTTGGAAGTTGGTTTAA+TGG - chr2.2:47311550-47311569 None:intergenic 30.0%
! CCAAATAATACCGTTTTCAT+GGG + chr2.2:47311447-47311466 MS.gene22522:CDS 30.0%
! GGTTGAAATTTTACAATCTC+AGG + chr2.2:47310730-47310749 MS.gene22522:intron 30.0%
! TGAAATTTTACAATCTCAGG+GGG + chr2.2:47310733-47310752 MS.gene22522:intron 30.0%
! TGATTTGCAAAGTTCAAACT+TGG - chr2.2:47308904-47308923 None:intergenic 30.0%
! TTTGTTGTCTTAGATTGTCA+AGG - chr2.2:47309185-47309204 None:intergenic 30.0%
!! GTTGATTTTAACATTGAAGG+AGG + chr2.2:47309126-47309145 MS.gene22522:CDS 30.0%
!! TATAGTTTCAAGTTGTACAG+CGG - chr2.2:47309304-47309323 None:intergenic 30.0%
!!! GGTGTTGATTTTAACATTGA+AGG + chr2.2:47309123-47309142 MS.gene22522:CDS 30.0%
!!! TTTTAAGACACAAGTTTCTG+AGG + chr2.2:47311662-47311681 MS.gene22522:CDS 30.0%
AAATATGCAAACTGGGAACT+TGG + chr2.2:47310402-47310421 MS.gene22522:intron 35.0%
AAGCATAATTCCTCCATAGT+TGG - chr2.2:47311565-47311584 None:intergenic 35.0%
ACATGTTAAGGAACTTAAGG+TGG + chr2.2:47310807-47310826 MS.gene22522:intron 35.0%
ACCAGATGATCTCATTTCTA+AGG + chr2.2:47311515-47311534 MS.gene22522:CDS 35.0%
ACCTTAGAAATGAGATCATC+TGG - chr2.2:47311519-47311538 None:intergenic 35.0%
ACTATGGAGGAATTATGCTT+TGG + chr2.2:47311565-47311584 MS.gene22522:CDS 35.0%
AGTTCTTAAGGCACATGTTA+AGG - chr2.2:47310919-47310938 None:intergenic 35.0%
ATGATCAAGACTTTAGCACA+TGG - chr2.2:47310428-47310447 None:intergenic 35.0%
CCCATGAAAACGGTATTATT+TGG - chr2.2:47311450-47311469 None:intergenic 35.0%
CTATGGAGGAATTATGCTTT+GGG + chr2.2:47311566-47311585 MS.gene22522:CDS 35.0%
CTTATTCTTTACGCACACAA+AGG - chr2.2:47309560-47309579 None:intergenic 35.0%
CTTGACAAAGCTATCAAAAC+TGG + chr2.2:47311306-47311325 MS.gene22522:CDS 35.0%
CTTTGTGTGCGTAAAGAATA+AGG + chr2.2:47309558-47309577 MS.gene22522:intron 35.0%
GAAGGTGAGATAAGAATAAC+TGG + chr2.2:47310621-47310640 MS.gene22522:intron 35.0%
GTTAAATAAAACGTGGTGTC+TGG - chr2.2:47309631-47309650 None:intergenic 35.0%
TGGGATAGATTTCATGATGT+TGG + chr2.2:47311585-47311604 MS.gene22522:CDS 35.0%
TTAGAAATGAGATCATCTGG+TGG - chr2.2:47311516-47311535 None:intergenic 35.0%
TTGATTTATGACAGGTGTAC+CGG - chr2.2:47311005-47311024 None:intergenic 35.0%
! AAGATGAAGAACCCTGTTTT+GGG - chr2.2:47309443-47309462 None:intergenic 35.0%
! AAGGAGGTACAAATCTTTAC+TGG + chr2.2:47309142-47309161 MS.gene22522:CDS 35.0%
! AGGAGGTACAAATCTTTACT+GGG + chr2.2:47309143-47309162 MS.gene22522:CDS 35.0%
! ATCAAAGCATCATCAAGTGA+TGG + chr2.2:47308757-47308776 MS.gene22522:CDS 35.0%
! ATCAACCTTTTATCTCTTGC+AGG + chr2.2:47311202-47311221 MS.gene22522:intron 35.0%
! TTGGAAGTTGGTTTAATGGA+AGG - chr2.2:47311546-47311565 None:intergenic 35.0%
!! GATGATGCTTTGATAGTGAA+GGG - chr2.2:47308751-47308770 None:intergenic 35.0%
!! TGATGATGCTTTGATAGTGA+AGG - chr2.2:47308752-47308771 None:intergenic 35.0%
!!! CAGTTTTGATAGCTTTGTCA+AGG - chr2.2:47311308-47311327 None:intergenic 35.0%
!!! GAAACAGTTTTAGCTTCTTC+TGG - chr2.2:47309039-47309058 None:intergenic 35.0%
AAACCAACTTCCAACTATGG+AGG + chr2.2:47311552-47311571 MS.gene22522:CDS 40.0%
AGGTACCTGCAAGAGATAAA+AGG - chr2.2:47311210-47311229 None:intergenic 40.0%
AGTGTCACATGTTGATGTCA+AGG - chr2.2:47308824-47308843 None:intergenic 40.0%
ATGACATGGGGATATCAGAA+AGG - chr2.2:47309498-47309517 None:intergenic 40.0%
ATTCCTCCATAGTTGGAAGT+TGG - chr2.2:47311558-47311577 None:intergenic 40.0%
CTCAAAGTGAAACAACATGC+TGG + chr2.2:47310657-47310676 MS.gene22522:intron 40.0%
CTGTCAAGAAAAAGGGTTCA+AGG + chr2.2:47308975-47308994 MS.gene22522:CDS 40.0%
GCGTAAAGAATAAGGTTGCT+TGG + chr2.2:47309566-47309585 MS.gene22522:intron 40.0%
GCTTTGTCAAGGTAGTAATC+TGG - chr2.2:47311297-47311316 None:intergenic 40.0%
GGCACATGTTAAGGAACTTA+AGG + chr2.2:47310804-47310823 MS.gene22522:intron 40.0%
GGTGTGCAAGTGCAAATAAA+CGG + chr2.2:47310699-47310718 MS.gene22522:intron 40.0%
TTGACATCAACATGTGACAC+TGG + chr2.2:47308823-47308842 MS.gene22522:CDS 40.0%
! AAAGCATCATCAAGTGATGG+TGG + chr2.2:47308760-47308779 MS.gene22522:CDS 40.0%
! AGGTGCCTTCTTTTACGTTT+TGG - chr2.2:47308804-47308823 None:intergenic 40.0%
! CAGGTACTTTTACTTGTCTG+CGG + chr2.2:47311254-47311273 MS.gene22522:intron 40.0%
! GAAGATGAAGAACCCTGTTT+TGG - chr2.2:47309444-47309463 None:intergenic 40.0%
! TCTAAGGTAACACTTCCAAG+TGG - chr2.2:47309102-47309121 None:intergenic 40.0%
! TTACTTTCCCTCAGAGCATT+AGG + chr2.2:47309000-47309019 MS.gene22522:CDS 40.0%
! TTGGCTTTCCTCAATGTCTT+TGG + chr2.2:47308865-47308884 MS.gene22522:CDS 40.0%
!! CGGATTCGAGTTTTGTACAA+GGG - chr2.2:47308945-47308964 None:intergenic 40.0%
!! CTTGGAAGTGTTACCTTAGA+TGG + chr2.2:47309102-47309121 MS.gene22522:CDS 40.0%
!! TCGGATTCGAGTTTTGTACA+AGG - chr2.2:47308946-47308965 None:intergenic 40.0%
!! TTAACTTGTGCTCTTAGCAG+TGG + chr2.2:47309804-47309823 MS.gene22522:intron 40.0%
AAAGAAACTGGCATCTGGCT+TGG + chr2.2:47309595-47309614 MS.gene22522:intron 45.0%
AACATGTGCCCTAAGAACCA+GGG - chr2.2:47310794-47310813 None:intergenic 45.0%
AAGCCAGATGCCAGTTTCTT+TGG - chr2.2:47309596-47309615 None:intergenic 45.0%
AGACCAAAGAAACTGGCATC+TGG + chr2.2:47309590-47309609 MS.gene22522:intron 45.0%
AGCTTCTTCTGGTGAGCTAA+GGG - chr2.2:47309028-47309047 None:intergenic 45.0%
AGTGGTCAATTTGGTCCACT+TGG + chr2.2:47309084-47309103 MS.gene22522:CDS 45.0%
CTTGACCACATCCCAAAACA+GGG + chr2.2:47309429-47309448 MS.gene22522:intron 45.0%
GCTTGGTAGACCAAAGAAAC+TGG + chr2.2:47309583-47309602 MS.gene22522:intron 45.0%
GCTTTCCTCAATGTCTTTGG+AGG + chr2.2:47308868-47308887 MS.gene22522:CDS 45.0%
GGTGGTATATAACCACCACT+AGG - chr2.2:47311498-47311517 None:intergenic 45.0%
GGTTCTTAGGGCACATGTTA+AGG + chr2.2:47310795-47310814 MS.gene22522:intron 45.0%
TAACATGTGCCCTAAGAACC+AGG - chr2.2:47310795-47310814 None:intergenic 45.0%
TAGCTTCTTCTGGTGAGCTA+AGG - chr2.2:47309029-47309048 None:intergenic 45.0%
TCACCTTCTGACGCATAAAG+AGG - chr2.2:47310609-47310628 None:intergenic 45.0%
TCTTGACCACATCCCAAAAC+AGG + chr2.2:47309428-47309447 MS.gene22522:intron 45.0%
TGCTGGTAGTCCCATGAAAA+CGG - chr2.2:47311460-47311479 None:intergenic 45.0%
TGGGGCCAAAACGTAAAAGA+AGG + chr2.2:47308796-47308815 MS.gene22522:CDS 45.0%
TTACTGGGATGACCTTGCTA+GGG + chr2.2:47309158-47309177 MS.gene22522:CDS 45.0%
TTCCTCCTCCAAAGACATTG+AGG - chr2.2:47308876-47308895 None:intergenic 45.0%
TTTACTGGGATGACCTTGCT+AGG + chr2.2:47309157-47309176 MS.gene22522:CDS 45.0%
! AAAACGTGGTGTCTGGTCTT+TGG - chr2.2:47309624-47309643 None:intergenic 45.0%
! AAGAAACTGGCATCTGGCTT+GGG + chr2.2:47309596-47309615 MS.gene22522:intron 45.0%
! TTCCTCAATGTCTTTGGAGG+AGG + chr2.2:47308871-47308890 MS.gene22522:CDS 45.0%
!! CAAGTTTCTGAGGCAATAGC+TGG + chr2.2:47311672-47311691 MS.gene22522:CDS 45.0%
!! GAGTTTTGTACAAGGGCTCT+CGG - chr2.2:47308938-47308957 None:intergenic 45.0%
!!! AAGAACCCTGTTTTGGGATG+TGG - chr2.2:47309437-47309456 None:intergenic 45.0%
!!! AAATATCTTTTTTATAAAAT+TGG - chr2.2:47309400-47309419 None:intergenic 5.0%
!!! AATATCTTTTTTATAAAATT+GGG - chr2.2:47309399-47309418 None:intergenic 5.0%
AAGGGCTCCTAATGCTCTGA+GGG - chr2.2:47309010-47309029 None:intergenic 50.0%
ACATGTGCCCTAAGAACCAG+GGG - chr2.2:47310793-47310812 None:intergenic 50.0%
AGGCACGTGCAACACAGATT+TGG - chr2.2:47309540-47309559 None:intergenic 50.0%
AGGTGCACACCCTCAATAAG+AGG + chr2.2:47310759-47310778 MS.gene22522:intron 50.0%
GATTGTCAAGGTCCCTAGCA+AGG - chr2.2:47309173-47309192 None:intergenic 50.0%
GCACCTCTTTATGCGTCAGA+AGG + chr2.2:47310603-47310622 MS.gene22522:intron 50.0%
GGCACGTGCAACACAGATTT+GGG - chr2.2:47309539-47309558 None:intergenic 50.0%
TAAGGGCTCCTAATGCTCTG+AGG - chr2.2:47309011-47309030 None:intergenic 50.0%
TGCACACCCTCAATAAGAGG+TGG + chr2.2:47310762-47310781 MS.gene22522:intron 50.0%
! TTTACAATCTCAGGGGGTGC+AGG + chr2.2:47310739-47310758 MS.gene22522:intron 50.0%
!! TACGTTTTGGCCCCAGTAGA+CGG - chr2.2:47308791-47308810 None:intergenic 50.0%
ATGGTGGCATTGCCGTCTAC+TGG + chr2.2:47308776-47308795 MS.gene22522:CDS 55.0%
CCACCACTAGGAGCTGCATT+AGG - chr2.2:47311486-47311505 None:intergenic 55.0%
CCTAATGCAGCTCCTAGTGG+TGG + chr2.2:47311483-47311502 MS.gene22522:CDS 55.0%
CTCTTAGCAGTGGCGTATCC+AGG + chr2.2:47309814-47309833 MS.gene22522:intron 55.0%
GCACCTAATGCAGCTCCTAG+TGG + chr2.2:47311480-47311499 MS.gene22522:CDS 55.0%
GCGGAGCCACCTCTTATTGA+GGG - chr2.2:47310771-47310790 None:intergenic 55.0%
TGGTGGCATTGCCGTCTACT+GGG + chr2.2:47308777-47308796 MS.gene22522:CDS 55.0%
AGTGGCGTATCCAGGACCCT+GGG + chr2.2:47309822-47309841 MS.gene22522:intron 60.0%
GGCGGAGCCACCTCTTATTG+AGG - chr2.2:47310772-47310791 None:intergenic 60.0%
GGTGGCATTGCCGTCTACTG+GGG + chr2.2:47308778-47308797 MS.gene22522:CDS 60.0%
GTATCCAGGACCCTGGGTCA+GGG + chr2.2:47309828-47309847 MS.gene22522:intron 60.0%
TATCCAGGACCCTGGGTCAG+GGG + chr2.2:47309829-47309848 MS.gene22522:intron 60.0%
TGTGCCCTAAGAACCAGGGG+CGG - chr2.2:47310790-47310809 None:intergenic 60.0%
! GGAGCTGCATTAGGTGCTGC+TGG - chr2.2:47311477-47311496 None:intergenic 60.0%
!! GTCAGGGGGTGCGGAGTTTT+TGG + chr2.2:47309844-47309863 MS.gene22522:intron 60.0%
ATAAGAGGTGGCTCCGCCCC+TGG + chr2.2:47310774-47310793 MS.gene22522:intron 65.0%
ATCCAGGACCCTGGGTCAGG+GGG + chr2.2:47309830-47309849 MS.gene22522:intron 65.0%
CAGTGGCGTATCCAGGACCC+TGG + chr2.2:47309821-47309840 MS.gene22522:intron 65.0%
CGTATCCAGGACCCTGGGTC+AGG + chr2.2:47309827-47309846 MS.gene22522:intron 65.0%
! GGCTCCGCCCCTGGTTCTTA+GGG + chr2.2:47310783-47310802 MS.gene22522:intron 65.0%
! TGGCTCCGCCCCTGGTTCTT+AGG + chr2.2:47310782-47310801 MS.gene22522:intron 65.0%
AACTCCGCACCCCCTGACCC+AGG - chr2.2:47309842-47309861 None:intergenic 70.0%
ACTCCGCACCCCCTGACCCA+GGG - chr2.2:47309841-47309860 None:intergenic 70.0%
CACCCCCTGACCCAGGGTCC+TGG - chr2.2:47309835-47309854 None:intergenic 75.0%
GGACCCTGGGTCAGGGGGTG+CGG + chr2.2:47309835-47309854 MS.gene22522:intron 75.0%


Chromosome Type Strat End Strand Name
chr2.2 gene 47308697 47311734 47308697 ID=MS.gene22522
chr2.2 mRNA 47308697 47311734 47308697 ID=MS.gene22522.t1;Parent=MS.gene22522
chr2.2 exon 47308697 47309208 47308697 ID=MS.gene22522.t1.exon1;Parent=MS.gene22522.t1
chr2.2 CDS 47308697 47309208 47308697 ID=cds.MS.gene22522.t1;Parent=MS.gene22522.t1
chr2.2 exon 47311257 47311734 47311257 ID=MS.gene22522.t1.exon2;Parent=MS.gene22522.t1
chr2.2 CDS 47311257 47311734 47311257 ID=cds.MS.gene22522.t1;Parent=MS.gene22522.t1
Gene Sequence

>MS.gene22522

ATGGATACAAAAAGGCAAGCATTGCTCCTTTTGTTAGTCTTGACAATATTTCCCTTCACTATCAAAGCATCATCAAGTGATGGTGGCATTGCCGTCTACTGGGGCCAAAACGTAAAAGAAGGCACCTTGACATCAACATGTGACACTGGTAATTTCGAGATTGTACTCTTGGCTTTCCTCAATGTCTTTGGAGGAGGAACTGTCCCAAGTTTGAACTTTGCAAATCATTGTGGTCATGCCGAGAGCCCTTGTACAAAACTCGAATCCGAAATAAAACACTGTCAAGAAAAAGGGTTCAAGGTTTTACTTTCCCTCAGAGCATTAGGAGCCCTTAGCTCACCAGAAGAAGCTAAAACTGTTTCTGATTACCTCTATACTAACTTTCTTAGTGGTCAATTTGGTCCACTTGGAAGTGTTACCTTAGATGGTGTTGATTTTAACATTGAAGGAGGTACAAATCTTTACTGGGATGACCTTGCTAGGGACCTTGACAATCTAAGACAACAAAACAGGTAATATTTTTTGAATGACAAGTTTTAGCATGCTAGTACTAGCTAGTAGTACCCGATTCAAAAAAAAAGTACTAGCTAGTAGTACAACTTGACCGCTGTACAACTTGAAACTATAAAACTAAAAATATATATGTAAAATAAAGTTATAAAAAAAAAATGAAGATGATGAAGAAAAAGTTGACCACATCCCAATTTTATAAAAAAGATATTTTTTTTTCTTCTTGACCACATCCCAAAACAGGGTTCTTCATCTTCTTCTACGATTAGTTCTTACATATGAAGTGCTCCTTTCTGATATCCCCATGTCATGATATATTTCTTTATGAGCCCAAATCTGTGTTGCACGTGCCTTTGTGTGCGTAAAGAATAAGGTTGCTTGGTAGACCAAAGAAACTGGCATCTGGCTTGGGAACCAAAGACCAGACACCACGTTTTATTTAACATCCTAATGAACAACCATATTTTTTCAAAGAAAAATATGTATGGACTCGAAAATTATAGCAAAAGAGAGAGAAAATATAATGTGAGTATAAGAGGTAGTTAAATCACCTAAAGTGTATAATGTGAATATGAGAAAATATAATATAGGAAAATGTTAACTTGTGCTCTTAGCAGTGGCGTATCCAGGACCCTGGGTCAGGGGGTGCGGAGTTTTTGGCGTAAATAATTTAAACAACAAAATTTATAATTTCATAATTTTTTACTCAATCAAAAATTATTTTTTCCAAACATAAATAAAAGTAAGCACATAAATTTTCATGTCTTTCATGATTCAAAAGTATGTAGACATTATTAATTTAGAAGTATAATTAGATAAATAATTAATATAAAATTAGAAAAGCAAAATGATATTTATTTTAGACATTTTTATATGACAAATTGATGCTCCATAATAATTTTAGAATGGATATAGTAAAAAAGGATAATGAGTGAAATAAACAAAAATGATTTAATTTGTTGAGTATTATATGAACTAGTATTGGATGGACACAATATAAGGGTACACTCACATATTTGAGAAAAGATAAAAAATAAGAAAGATTAAATGATATCTATTTTAAAATAATTGAATGATATTTTTTTTGAAGAAAAATGATATATATTTTGTTATGTGTGTGCTGAAAGATTGTGCCCAAATATTTGTAACATAAGTATTTTCCGTATTTCCCTAAAAAAAAAGTATTTTCCGTATTAAATATGCAAACTGGGAACTTGGCCATGTGCTAAAGTCTTGATCATGACATTACATTTACTTTTATTTTAACCCTGAGAACTTAATTTGAAGTGATACAAATAAAAAAAATGAGAAAAAGTAATCTAAACACTTTTAACTTAAGAAAGTAGAATGGTAGAAGCTTGAGTGTGACCGAAGACAAGTGAAAATTTTTGTCAGAGCACCTCTTTATGCGTCAGAAGGTGAGATAAGAATAACTGGATAAGTGCAAAATCTCAAAGTGAAACAACATGCTGGTGCAAAATAAATTTCTCAGGGTGTGCAAGTGCAAATAAACGGATATAATTGGTTGAAATTTTACAATCTCAGGGGGTGCAGGTGCACACCCTCAATAAGAGGTGGCTCCGCCCCTGGTTCTTAGGGCACATGTTAAGGAACTTAAGGTGGAATTTTTGTTTATGAAATTGTGTAAATTTAATATTATAAAGATTGAAAATTAATGTTTCATAATAAATATTTACCATTTATGGAATCCTTAACATGTGCCTTAAGAACTCAAGTTAGCATAATGCTATAATATATTGGGAGTTTCTACGGTACATCTTTAAATTAAAGTGTACCGGTACACCTGTCATAAATCAATAAATTAACGATAATTTTTATGTAATAAAGAGATATTTATCGACTTTTATATTTTAGAAAATATTATCCCATAAAAAAAAACATCATTTCATTCTTTATAAGAATTATACTAAATTTTTTAATATTTTATTGAAAAAATAATCAATATTTCTTCTTTGCTTACACCCTAATTAACAAATCAACCTTTTATCTCTTGCAGGTACCTCTCTCAAAAAAAAAAAATCTCTTGCAGGTACTTTTACTTGTCTGCGGCACCCCAATGTTATATGCCAGATTACTACCTTGACAAAGCTATCAAAACTGGCTTGTTTGATTATGTACTTGTTAAATTCTACAATAACCCTCCATGTCAATATGATAAAAAAAACTCAGATGCTACATTGCTCTTAAAATCATGGAATGCTTGGACATCTTTAGTTCTACCAAATAATACCGTTTTCATGGGACTACCAGCAGCACCTAATGCAGCTCCTAGTGGTGGTTATATACCACCAGATGATCTCATTTCTAAGGTTCTTCCTTCCATTAAACCAACTTCCAACTATGGAGGAATTATGCTTTGGGATAGATTTCATGATGTTGGAAATAATGATTACAGCTATCAGATAAAGGAGCATGTCAAACGATCTGTTCTTCGTTTTAAGACACAAGTTTCTGAGGCAATAGCTGGATGTATCTCAGCAGCTTTGAACTCCATGTTTACGAATTAA
Protein sequence

>MS.gene22522.t1

MDTKRQALLLLLVLTIFPFTIKASSSDGGIAVYWGQNVKEGTLTSTCDTGNFEIVLLAFLNVFGGGTVPSLNFANHCGHAESPCTKLESEIKHCQEKGFKVLLSLRALGALSSPEEAKTVSDYLYTNFLSGQFGPLGSVTLDGVDFNIEGGTNLYWDDLARDLDNLRQQNRYFYLSAAPQCYMPDYYLDKAIKTGLFDYVLVKFYNNPPCQYDKKNSDATLLLKSWNAWTSLVLPNNTVFMGLPAAPNAAPSGGYIPPDDLISKVLPSIKPTSNYGGIMLWDRFHDVGNNDYSYQIKEHVKRSVLRFKTQVSEAIAGCISAALNSMFTN