AlfalfaGEDB | MS.gene22901

M. sativa cultivar XinJiangDaYe / MS.gene22901

KEGG: -
GO: GO:0003674 GO:0003824
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene22901.t1	XP_013450571.1	93.3	75	5	0	1	75	272	346	2.70E-31	144.1

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene22901.t1	Q8RXU4	58.7	75	31	0	1	75	272	346	3.5e-19	95.1

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene22901.t1	A0A072U4C8	93.3	75	5	0	1	75	272	346	2.0e-31	144.1

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene050559	MS.gene22901	0.835153	1.98E-56	-1.69E-46
MS.gene050561	MS.gene22901	0.841076	5.92E-58	-1.69E-46
MS.gene050562	MS.gene22901	0.812397	4.33E-51	-1.69E-46
MS.gene053998	MS.gene22901	0.808353	3.23E-50	-1.69E-46
MS.gene05415	MS.gene22901	0.804827	1.79E-49	-1.69E-46
MS.gene055227	MS.gene22901	0.807681	4.49E-50	-1.69E-46
MS.gene055818	MS.gene22901	0.814283	1.67E-51	-1.69E-46
MS.gene058010	MS.gene22901	0.802995	4.31E-49	-1.69E-46
MS.gene058979	MS.gene22901	0.808886	2.48E-50	-1.69E-46
MS.gene060322	MS.gene22901	0.811235	7.75E-51	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 18 sgRNAs with CRISPR-Local

Find 20 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ATACACTTTGTCGTGCTTTC+AGG	0.173516	7.4:-2047729	None:intergenic
CTACAAAATACTTACAATAT+TGG	0.205905	7.4:+2048106	None:intergenic
GCACATCGCTATCTGATATT+TGG	0.274562	7.4:+2047753	None:intergenic
ATTGAAATTGAAGAGAATTC+AGG	0.311218	7.4:-2047988	MS.gene22901:CDS
TCCAAGGACTTAGAAGAATA+TGG	0.358787	7.4:-2047946	MS.gene22901:CDS
GCCATATTCTTCTAAGTCCT+TGG	0.397824	7.4:+2047945	None:intergenic
TCCTTGGATAGCTTTGCTGC+TGG	0.432710	7.4:+2047961	None:intergenic
GGCATCCTTCTCATGCCAAT+CGG	0.513035	7.4:-2047925	MS.gene22901:CDS
CGCTATCTGATATTTGGTGG+TGG	0.515490	7.4:+2047759	None:intergenic
CATCGCTATCTGATATTTGG+TGG	0.529995	7.4:+2047756	None:intergenic
TGAAATCAAAGGACTGAGAG+TGG	0.556209	7.4:-2048147	MS.gene22901:CDS
AATATTGGTTTCCACAGAAG+AGG	0.561141	7.4:+2048121	None:intergenic
GATGGATTGAATGAAATCAA+AGG	0.567749	7.4:-2048158	MS.gene22901:CDS
TCATGCCAATCGGATCATCA+AGG	0.575646	7.4:-2047915	MS.gene22901:intron
CAAGACCTTGATGATCCGAT+TGG	0.596889	7.4:+2047910	None:intergenic
TCCAGCAGCAAAGCTATCCA+AGG	0.609285	7.4:-2047962	MS.gene22901:CDS
GAGAGTGGATACCTCTTCTG+TGG	0.618793	7.4:-2048132	MS.gene22901:CDS
ATGATCCGATTGGCATGAGA+AGG	0.633762	7.4:+2047920	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	GAGTAAATTACTTTAATAAA+AGG	+	chr7.4:2048045-2048064	None:intergenic	15.0%
!!	CTACAAAATACTTACAATAT+TGG	+	chr7.4:2047784-2047803	None:intergenic	20.0%
!!	CTTCAATTTCAATATATATC+TGG	+	chr7.4:2047892-2047911	None:intergenic	20.0%
!	ACTAGTATGAATGAAATGAA+TGG	+	chr7.4:2047810-2047829	None:intergenic	25.0%
!	ATTGAAATTGAAGAGAATTC+AGG	-	chr7.4:2047899-2047918	MS.gene22901:intron	25.0%
	TCTTATCGTATCAGTTTCAT+TGG	-	chr7.4:2048072-2048091	MS.gene22901:intron	30.0%
	TCCAAGGACTTAGAAGAATA+TGG	-	chr7.4:2047941-2047960	MS.gene22901:CDS	35.0%
!!	AATATTGGTTTCCACAGAAG+AGG	+	chr7.4:2047769-2047788	None:intergenic	35.0%
	CATCGCTATCTGATATTTGG+TGG	+	chr7.4:2048134-2048153	None:intergenic	40.0%
	GCACATCGCTATCTGATATT+TGG	+	chr7.4:2048137-2048156	None:intergenic	40.0%
	GCCATATTCTTCTAAGTCCT+TGG	+	chr7.4:2047945-2047964	None:intergenic	40.0%
	TGAAATCAAAGGACTGAGAG+TGG	-	chr7.4:2047740-2047759	MS.gene22901:CDS	40.0%
	ATGATCCGATTGGCATGAGA+AGG	+	chr7.4:2047970-2047989	None:intergenic	45.0%
	TCATGCCAATCGGATCATCA+AGG	-	chr7.4:2047972-2047991	MS.gene22901:CDS	45.0%
!	CAAGACCTTGATGATCCGAT+TGG	+	chr7.4:2047980-2047999	None:intergenic	45.0%
!	CGCTATCTGATATTTGGTGG+TGG	+	chr7.4:2048131-2048150	None:intergenic	45.0%
	GAGAGTGGATACCTCTTCTG+TGG	-	chr7.4:2047755-2047774	MS.gene22901:CDS	50.0%
	GGCATCCTTCTCATGCCAAT+CGG	-	chr7.4:2047962-2047981	MS.gene22901:CDS	50.0%
	TCCAGCAGCAAAGCTATCCA+AGG	-	chr7.4:2047925-2047944	MS.gene22901:CDS	50.0%
!	TCCTTGGATAGCTTTGCTGC+TGG	+	chr7.4:2047929-2047948	None:intergenic	50.0%

Chromosome	Type	Strat	End	Strand	Name
chr7.4	gene	2047730	2048179	2047730	ID=MS.gene22901
chr7.4	mRNA	2047730	2048179	2047730	ID=MS.gene22901.t1;Parent=MS.gene22901
chr7.4	exon	2048121	2048179	2048121	ID=MS.gene22901.t1.exon1;Parent=MS.gene22901.t1
chr7.4	CDS	2048121	2048179	2048121	ID=cds.MS.gene22901.t1;Parent=MS.gene22901.t1
chr7.4	exon	2047916	2048016	2047916	ID=MS.gene22901.t1.exon2;Parent=MS.gene22901.t1
chr7.4	CDS	2047916	2048016	2047916	ID=cds.MS.gene22901.t1;Parent=MS.gene22901.t1
chr7.4	exon	2047730	2047796	2047730	ID=MS.gene22901.t1.exon3;Parent=MS.gene22901.t1
chr7.4	CDS	2047730	2047796	2047730	ID=cds.MS.gene22901.t1;Parent=MS.gene22901.t1

Gene Sequence

>MS.gene22901

ATGGATTGAATGAAATCAAAGGACTGAGAGTGGATACCTCTTCTGTGGAAACCAATATTGTAAGTATTTTGTAGAGTCCATTCATTTCATTCATACTAGTACAACAATTGCATTATTATTTCTCTGATTACTTTTGTGTTTAATCAATAATCTCTGATACCAGATATATATTGAAATTGAAGAGAATTCAGGAACTCCAGCAGCAAAGCTATCCAAGGACTTAGAAGAATATGGCATCCTTCTCATGCCAATCGGATCATCAAGGTCTTGTCTGTCACACTCTGCATTGTTACTAATAATATTATTGATTAACCTTTTATTAAAGTAATTTACTCATATGAATCTTATCGTATCAGTTTCATTGGTTCATTTTCTCTTGACAGATTGAGAGTTGTATTCCACCACCAAATATCAGATAGCGATGTGCGATACACTTTGTCGTGCTTTCAG

Protein sequence

>MS.gene22901.t1

MDUMKSKDUEWIPLLWKPILYILKLKRIQELQQQSYPRTUKNMASFSCQSDHQDUELYSTTKYQIAMCDTLCRAF