AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene23262

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene23262.t1 MTR_4g070080 97.333 75 2 0 1 75 1 75 2.49e-51 158
MS.gene23262.t1 MTR_4g070080 97.333 75 2 0 1 75 1 75 2.42e-50 154
MS.gene23262.t1 MTR_4g070190 89.189 74 8 0 2 75 4 77 1.27e-44 141
MS.gene23262.t1 MTR_4g070140 82.432 74 13 0 2 75 4 77 1.06e-40 132
MS.gene23262.t1 MTR_3g075500 57.353 68 29 0 7 74 207 274 1.30e-23 90.5
MS.gene23262.t1 MTR_4g109540 53.623 69 32 0 7 75 43 111 1.66e-22 87.4
MS.gene23262.t1 MTR_4g104470 54.412 68 31 0 7 74 217 284 3.38e-22 87.0
MS.gene23262.t1 MTR_4g104470 36.111 72 46 0 5 76 121 192 6.74e-11 56.2
MS.gene23262.t1 MTR_3g075500 57.812 64 27 0 7 70 207 270 3.68e-22 86.3
MS.gene23262.t1 MTR_7g013730 50.000 70 35 0 7 76 41 110 5.26e-22 85.9
MS.gene23262.t1 MTR_3g084040 50.725 69 33 1 7 75 37 104 4.88e-21 80.9
MS.gene23262.t1 MTR_4g109540 54.545 66 30 0 10 75 54 119 1.02e-20 82.8
MS.gene23262.t1 MTR_7g013740 45.588 68 37 0 7 74 41 108 3.23e-20 78.6
MS.gene23262.t1 MTR_1g064230 49.315 73 37 0 2 74 192 264 3.84e-20 81.3
MS.gene23262.t1 MTR_3g025880 50.685 73 35 1 7 78 45 117 1.65e-19 80.9
MS.gene23262.t1 MTR_3g084040 50.000 64 31 1 7 70 37 99 4.63e-19 74.7
MS.gene23262.t1 MTR_3g080130 45.588 68 37 0 7 74 37 104 9.27e-19 74.7
MS.gene23262.t1 MTR_7g105260 43.836 73 41 0 4 76 30 102 2.38e-18 73.6
MS.gene23262.t1 MTR_3g014000 50.685 73 25 2 9 74 37 105 9.81e-18 72.0
MS.gene23262.t1 MTR_1g045510 40.278 72 43 0 3 74 201 272 4.39e-17 73.2
MS.gene23262.t1 MTR_7g117495 50.847 59 29 0 7 65 19 77 4.11e-16 70.1
MS.gene23262.t1 MTR_7g117495 50.847 59 29 0 7 65 19 77 4.25e-16 70.1
MS.gene23262.t1 MTR_3g092490 45.455 77 36 3 1 74 20 93 7.19e-16 68.2
MS.gene23262.t1 MTR_3g092490 49.254 67 30 2 1 66 20 83 8.21e-16 69.3
MS.gene23262.t1 MTR_3g092490 49.254 67 30 2 1 66 20 83 8.31e-16 69.3
MS.gene23262.t1 MTR_4g035310 43.243 74 37 2 2 70 11 84 2.63e-15 68.2
MS.gene23262.t1 MTR_4g035310 43.243 74 37 2 2 70 11 84 2.76e-15 68.2
MS.gene23262.t1 MTR_3g029580 40.789 76 45 0 1 76 27 102 3.23e-15 65.5
MS.gene23262.t1 MTR_4g081520 39.474 76 46 0 1 76 46 121 4.56e-15 65.5
MS.gene23262.t1 MTR_6g477870 40.000 70 42 0 7 76 96 165 5.60e-15 65.9
MS.gene23262.t1 MTR_7g099940 40.000 70 42 0 7 76 36 105 7.34e-15 64.7
MS.gene23262.t1 MTR_3g116130 42.857 63 35 1 2 63 24 86 1.31e-14 66.2
MS.gene23262.t1 MTR_5g064120 47.368 57 30 0 7 63 17 73 1.83e-14 65.9
MS.gene23262.t1 MTR_3g116130 42.857 63 35 1 2 63 24 86 1.97e-14 66.2
MS.gene23262.t1 MTR_8g024520 47.170 53 28 0 7 59 26 78 6.02e-14 64.7
MS.gene23262.t1 MTR_8g024520 47.170 53 28 0 7 59 26 78 6.11e-14 64.7
MS.gene23262.t1 MTR_4g036415 46.970 66 35 0 9 74 454 519 7.85e-14 64.7
MS.gene23262.t1 MTR_3g029580 40.000 70 42 0 1 70 27 96 1.21e-13 61.6
MS.gene23262.t1 MTR_7g106440 38.889 72 44 0 3 74 209 280 1.35e-13 63.9
MS.gene23262.t1 MTR_4g063560 36.765 68 43 0 7 74 34 101 1.45e-13 60.8
MS.gene23262.t1 MTR_3g055990 47.059 51 27 0 7 57 7 57 4.11e-13 62.8
MS.gene23262.t1 MTR_3g055990 47.059 51 27 0 7 57 7 57 4.53e-13 62.8
MS.gene23262.t1 MTR_1g080150 34.848 66 43 0 5 70 131 196 6.40e-13 61.6
MS.gene23262.t1 MTR_2g096560 35.714 70 45 0 7 76 8 77 7.18e-13 58.5
MS.gene23262.t1 MTR_3g082650 47.458 59 31 0 7 65 19 77 9.70e-13 61.6
MS.gene23262.t1 MTR_3g082650 40.299 67 40 0 7 73 107 173 5.03e-11 56.6
MS.gene23262.t1 MTR_5g017280 41.429 70 38 1 5 74 3 69 1.86e-12 60.8
MS.gene23262.t1 MTR_6g477870 37.500 64 40 0 7 70 96 159 1.98e-12 58.9
MS.gene23262.t1 MTR_5g017280 41.429 70 38 1 5 74 3 69 2.01e-12 60.8
MS.gene23262.t1 MTR_2g026180 48.000 50 26 0 7 56 5 54 3.13e-12 60.5
MS.gene23262.t1 MTR_7g102730 47.541 61 31 1 9 69 101 160 4.34e-12 60.1
MS.gene23262.t1 MTR_3g065190 45.070 71 38 1 3 72 109 179 4.74e-12 59.7
MS.gene23262.t1 MTR_7g088140 42.373 59 34 0 7 65 128 186 6.15e-12 59.3
MS.gene23262.t1 MTR_7g088140 42.373 59 34 0 7 65 128 186 6.15e-12 59.3
MS.gene23262.t1 MTR_1g073690 33.898 59 39 0 7 65 7 65 7.10e-12 59.3
MS.gene23262.t1 MTR_1g056520 33.333 72 48 0 3 74 204 275 1.02e-11 58.5
MS.gene23262.t1 MTR_1g092860 35.294 68 44 0 7 74 193 260 1.19e-11 58.5
MS.gene23262.t1 MTR_4g117350 36.765 68 43 0 7 74 72 139 1.75e-11 56.6
MS.gene23262.t1 MTR_4g117350 37.500 64 40 0 7 70 72 135 1.75e-11 56.2
MS.gene23262.t1 MTR_5g097580 50.000 48 24 0 7 54 7 54 3.11e-11 57.4
MS.gene23262.t1 MTR_5g097580 50.000 48 24 0 7 54 7 54 3.60e-11 57.4
MS.gene23262.t1 MTR_6g004810 47.458 59 31 0 7 65 46 104 3.84e-11 57.0
MS.gene23262.t1 MTR_6g004810 39.706 68 41 0 7 74 134 201 8.31e-11 56.2
MS.gene23262.t1 MTR_3g027140 36.986 73 46 0 3 75 156 228 9.89e-11 55.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene23262.t1 AT2G21660 82.192 73 13 0 3 75 5 77 1.79e-40 130
MS.gene23262.t1 AT2G21660 82.192 73 13 0 3 75 5 77 2.93e-40 130
MS.gene23262.t1 AT4G39260 71.053 76 22 0 1 76 1 76 1.71e-38 123
MS.gene23262.t1 AT4G39260 71.053 76 22 0 1 76 1 76 3.61e-38 123
MS.gene23262.t1 AT4G39260 71.053 76 22 0 1 76 1 76 1.26e-37 123
MS.gene23262.t1 AT4G39260 72.059 68 19 0 1 68 1 68 5.40e-34 112
MS.gene23262.t1 AT3G26420 56.757 74 32 0 3 76 4 77 1.53e-26 97.4
MS.gene23262.t1 AT3G23830 53.623 69 32 0 7 75 36 104 1.59e-24 89.4
MS.gene23262.t1 AT3G23830 53.623 69 32 0 7 75 36 104 1.59e-24 89.4
MS.gene23262.t1 AT5G61030 54.286 70 32 0 7 76 41 110 5.67e-24 92.0
MS.gene23262.t1 AT5G61030 54.286 70 32 0 7 76 41 110 5.67e-24 92.0
MS.gene23262.t1 AT5G50250 51.389 72 35 0 3 74 204 275 1.20e-22 88.2
MS.gene23262.t1 AT4G13850 50.000 72 36 0 4 75 33 104 3.71e-22 83.2
MS.gene23262.t1 AT4G13850 50.000 72 36 0 4 75 33 104 4.36e-22 83.6
MS.gene23262.t1 AT4G13850 50.000 72 36 0 4 75 33 104 5.45e-22 83.6
MS.gene23262.t1 AT4G13850 50.000 72 36 0 4 75 33 104 6.14e-22 83.6
MS.gene23262.t1 AT2G21690 55.714 70 31 0 2 71 3 72 2.71e-21 80.5
MS.gene23262.t1 AT4G24770 50.725 69 34 0 6 74 244 312 1.42e-20 83.2
MS.gene23262.t1 AT4G24770 40.278 72 43 0 5 76 149 220 7.52e-11 56.2
MS.gene23262.t1 AT4G24770 50.725 69 34 0 6 74 244 312 1.42e-20 83.2
MS.gene23262.t1 AT4G24770 40.278 72 43 0 5 76 149 220 7.52e-11 56.2
MS.gene23262.t1 AT1G74230 55.072 69 30 1 7 75 35 102 4.74e-20 81.3
MS.gene23262.t1 AT3G53460 47.059 68 36 0 7 74 279 346 4.81e-19 79.3
MS.gene23262.t1 AT3G53460 47.059 68 36 0 7 74 258 325 5.98e-19 79.0
MS.gene23262.t1 AT3G53460 47.059 68 36 0 7 74 258 325 5.98e-19 79.0
MS.gene23262.t1 AT3G53460 47.059 68 36 0 7 74 258 325 5.98e-19 79.0
MS.gene23262.t1 AT2G37220 47.059 68 36 0 7 74 205 272 6.57e-19 78.2
MS.gene23262.t1 AT1G18630 47.143 70 37 0 7 76 37 106 6.58e-19 75.5
MS.gene23262.t1 AT5G04280 50.000 66 33 0 7 72 8 73 2.88e-18 76.6
MS.gene23262.t1 AT5G47320 45.588 68 37 0 7 74 32 99 1.21e-17 73.6
MS.gene23262.t1 AT2G37510 47.143 70 37 0 7 76 35 104 1.30e-17 72.0
MS.gene23262.t1 AT4G13860 44.928 69 38 0 7 75 4 72 2.38e-17 69.7
MS.gene23262.t1 AT2G46780 48.387 62 32 0 7 68 23 84 6.03e-17 73.2
MS.gene23262.t1 AT2G46780 48.387 62 32 0 7 68 23 84 6.03e-17 73.2
MS.gene23262.t1 AT3G08000 46.377 69 37 0 7 75 42 110 8.62e-17 69.7
MS.gene23262.t1 AT1G60650 44.000 75 42 0 2 76 8 82 8.91e-17 72.4
MS.gene23262.t1 AT1G60650 44.000 75 42 0 2 76 8 82 8.91e-17 72.4
MS.gene23262.t1 AT5G06210 42.466 73 42 0 4 76 32 104 9.11e-17 69.7
MS.gene23262.t1 AT1G60000 37.500 72 45 0 3 74 174 245 3.90e-16 70.5
MS.gene23262.t1 AT1G78260 45.588 68 36 1 2 68 12 79 9.02e-16 69.3
MS.gene23262.t1 AT1G22330 46.774 62 33 0 7 68 18 79 1.01e-15 69.7
MS.gene23262.t1 AT1G78260 45.588 68 36 1 2 68 12 79 1.04e-15 69.3
MS.gene23262.t1 AT1G76460 49.123 57 29 0 7 63 25 81 1.64e-15 68.9
MS.gene23262.t1 AT1G20880 47.368 57 30 0 7 63 25 81 2.27e-15 68.6
MS.gene23262.t1 AT1G20880 47.368 57 30 0 7 63 25 81 2.27e-15 68.6
MS.gene23262.t1 AT1G20880 47.368 57 30 0 7 63 25 81 2.27e-15 68.6
MS.gene23262.t1 AT1G22910 41.333 75 39 2 1 70 7 81 2.57e-15 67.8
MS.gene23262.t1 AT1G22910 41.333 75 39 2 1 70 7 81 2.78e-15 67.8
MS.gene23262.t1 AT1G22910 41.333 75 39 2 1 70 7 81 5.53e-15 67.8
MS.gene23262.t1 AT5G54580 41.333 75 44 0 2 76 52 126 6.38e-15 65.5
MS.gene23262.t1 AT3G13224 44.286 70 36 1 7 73 20 89 2.07e-14 65.5
MS.gene23262.t1 AT3G13224 39.394 66 40 0 7 72 110 175 1.10e-11 58.2
MS.gene23262.t1 AT1G33470 43.750 64 35 1 1 63 1 64 2.40e-14 65.5
MS.gene23262.t1 AT1G33470 43.750 64 35 1 1 63 1 64 2.48e-14 65.5
MS.gene23262.t1 AT1G33470 43.750 64 35 1 1 63 1 64 2.50e-14 65.5
MS.gene23262.t1 AT5G11412 44.615 65 35 1 2 65 47 111 2.74e-14 63.9
MS.gene23262.t1 AT5G51300 42.857 70 40 0 5 74 479 548 9.72e-14 64.7
MS.gene23262.t1 AT5G51300 42.857 70 40 0 5 74 479 548 9.72e-14 64.7
MS.gene23262.t1 AT5G51300 42.857 70 40 0 5 74 479 548 9.72e-14 64.7
MS.gene23262.t1 AT3G54770 47.458 59 31 0 7 65 18 76 9.75e-14 63.9
MS.gene23262.t1 AT3G54770 47.458 59 31 0 7 65 18 76 9.84e-14 63.9
MS.gene23262.t1 AT3G13224 44.286 70 36 1 7 73 11 80 1.12e-13 64.3
MS.gene23262.t1 AT3G13224 39.394 66 40 0 7 72 101 166 1.52e-11 58.2
MS.gene23262.t1 AT3G46020 34.247 73 48 0 4 76 5 77 1.76e-13 60.5
MS.gene23262.t1 AT2G27330 35.714 70 45 0 9 78 24 93 2.11e-13 60.5
MS.gene23262.t1 AT3G13224 44.286 70 36 1 7 73 20 89 3.68e-13 62.8
MS.gene23262.t1 AT3G13224 39.394 66 40 0 7 72 110 175 3.83e-11 57.4
MS.gene23262.t1 AT5G53720 43.056 72 40 1 3 74 6 76 3.79e-13 59.3
MS.gene23262.t1 AT5G55550 50.000 50 25 0 7 56 7 56 4.03e-13 62.8
MS.gene23262.t1 AT1G01080 36.486 74 46 1 1 74 207 279 4.07e-13 62.4
MS.gene23262.t1 AT1G01080 36.486 74 46 1 1 74 208 280 4.09e-13 62.4
MS.gene23262.t1 AT5G55550 50.000 50 25 0 7 56 7 56 4.57e-13 62.8
MS.gene23262.t1 AT5G59865 40.580 69 41 0 7 75 27 95 5.23e-13 59.3
MS.gene23262.t1 AT5G19960 43.939 66 36 1 9 74 10 74 5.65e-13 62.4
MS.gene23262.t1 AT5G55550 50.000 50 25 0 7 56 7 56 5.80e-13 62.4
MS.gene23262.t1 AT5G55550 50.000 50 25 0 7 56 7 56 5.80e-13 62.4
MS.gene23262.t1 AT5G55550 50.000 50 25 0 7 56 7 56 5.80e-13 62.4
MS.gene23262.t1 AT5G55550 50.000 50 25 0 7 56 7 56 5.80e-13 62.4
MS.gene23262.t1 AT5G55550 50.000 50 25 0 7 56 7 56 6.97e-13 62.0
MS.gene23262.t1 AT5G55550 50.000 50 25 0 7 56 7 56 6.97e-13 62.0
MS.gene23262.t1 AT5G55550 50.000 50 25 0 7 56 7 56 6.97e-13 62.0
MS.gene23262.t1 AT5G55550 50.000 50 25 0 7 56 7 56 6.97e-13 62.0
MS.gene23262.t1 AT5G55550 50.000 50 25 0 7 56 7 56 6.97e-13 62.0
MS.gene23262.t1 AT3G04500 37.879 66 41 0 5 70 136 201 1.12e-12 60.8
MS.gene23262.t1 AT4G26650 41.176 68 38 1 7 74 16 81 1.51e-12 61.2
MS.gene23262.t1 AT4G13860 46.296 54 29 0 22 75 2 55 1.51e-12 57.0
MS.gene23262.t1 AT4G26650 41.176 68 38 1 7 74 13 78 1.62e-12 61.2
MS.gene23262.t1 AT3G07810 48.000 50 26 0 7 56 7 56 2.16e-12 60.8
MS.gene23262.t1 AT5G53680 41.935 62 36 0 7 68 14 75 2.18e-12 58.9
MS.gene23262.t1 AT5G40490 39.706 68 41 0 7 74 114 181 2.22e-12 60.8
MS.gene23262.t1 AT3G07810 48.000 50 26 0 7 56 7 56 2.22e-12 60.8
MS.gene23262.t1 AT1G01080 37.681 69 42 1 1 69 207 274 2.29e-12 60.5
MS.gene23262.t1 AT4G36960 41.667 72 39 1 4 75 1 69 2.68e-12 60.5
MS.gene23262.t1 AT4G36960 41.667 72 39 1 4 75 1 69 2.68e-12 60.5
MS.gene23262.t1 AT3G52380 34.722 72 47 0 3 74 216 287 2.68e-12 60.5
MS.gene23262.t1 AT1G17640 45.763 59 32 0 7 65 67 125 2.81e-12 60.5
MS.gene23262.t1 AT1G17640 49.091 55 28 0 7 61 158 212 5.07e-12 59.7
MS.gene23262.t1 AT1G17640 45.763 59 32 0 7 65 67 125 2.81e-12 60.5
MS.gene23262.t1 AT1G17640 49.091 55 28 0 7 61 158 212 5.07e-12 59.7
MS.gene23262.t1 AT1G17640 45.763 59 32 0 7 65 67 125 2.81e-12 60.5
MS.gene23262.t1 AT1G17640 49.091 55 28 0 7 61 158 212 5.07e-12 59.7
MS.gene23262.t1 AT5G47620 48.000 50 26 0 7 56 7 56 2.88e-12 60.5
MS.gene23262.t1 AT5G47620 48.000 50 26 0 7 56 7 56 3.72e-12 60.1
MS.gene23262.t1 AT5G47620 48.000 50 26 0 7 56 7 56 3.72e-12 60.1
MS.gene23262.t1 AT5G47620 48.000 50 26 0 7 56 7 56 3.72e-12 60.1
MS.gene23262.t1 AT3G52150 39.726 73 44 0 3 75 174 246 4.33e-12 59.3
MS.gene23262.t1 AT3G52150 39.726 73 44 0 3 75 174 246 4.33e-12 59.3
MS.gene23262.t1 AT5G40490 47.619 63 33 0 3 65 39 101 4.84e-12 59.7
MS.gene23262.t1 AT5G40490 39.706 68 41 0 7 74 131 198 1.79e-11 58.2
MS.gene23262.t1 AT2G37510 34.737 95 37 1 7 76 35 129 6.44e-12 57.8
MS.gene23262.t1 AT1G54080 41.538 65 38 0 6 70 148 212 6.49e-12 59.3
MS.gene23262.t1 AT4G14300 38.462 65 40 0 7 71 7 71 7.19e-12 59.3
MS.gene23262.t1 AT4G14300 38.462 65 40 0 7 71 7 71 7.19e-12 59.3
MS.gene23262.t1 AT3G14100 40.000 65 39 0 6 70 144 208 8.05e-12 59.3
MS.gene23262.t1 AT1G58470 46.154 52 28 0 6 57 6 57 1.32e-11 58.5
MS.gene23262.t1 AT1G58470 46.154 52 28 0 6 57 6 57 1.32e-11 58.5
MS.gene23262.t1 AT2G22100 43.860 57 32 0 9 65 166 222 1.61e-11 58.2
MS.gene23262.t1 AT2G33410 35.593 59 38 0 7 65 7 65 2.99e-11 57.4
MS.gene23262.t1 AT4G34110 41.538 65 37 1 9 73 218 281 3.24e-11 57.4
MS.gene23262.t1 AT3G20930 35.294 68 44 0 7 74 283 350 4.28e-11 57.0
MS.gene23262.t1 AT4G27000 43.284 67 37 1 5 70 172 238 4.53e-11 57.0
MS.gene23262.t1 AT2G41060 41.935 62 36 0 4 65 126 187 5.23e-11 57.0
MS.gene23262.t1 AT2G41060 41.935 62 36 0 4 65 126 187 5.23e-11 57.0
MS.gene23262.t1 AT2G41060 41.935 62 36 0 4 65 126 187 5.23e-11 57.0
MS.gene23262.t1 AT3G06970 38.983 59 36 0 7 65 13 71 6.10e-11 56.6
MS.gene23262.t1 AT3G56860 38.710 62 38 0 4 65 138 199 7.11e-11 56.6
MS.gene23262.t1 AT3G56860 38.710 62 38 0 4 65 138 199 7.11e-11 56.6
MS.gene23262.t1 AT3G56860 38.710 62 38 0 4 65 138 199 7.11e-11 56.6
MS.gene23262.t1 AT3G56860 38.710 62 38 0 4 65 138 199 7.11e-11 56.6
MS.gene23262.t1 AT3G56860 38.710 62 38 0 4 65 138 199 7.11e-11 56.6
MS.gene23262.t1 AT3G56860 38.710 62 38 0 4 65 138 199 7.11e-11 56.6
MS.gene23262.t1 AT3G56860 38.710 62 38 0 4 65 138 199 7.11e-11 56.6
MS.gene23262.t1 AT3G56860 38.710 62 38 0 4 65 138 199 7.11e-11 56.6
MS.gene23262.t1 AT3G56860 38.710 62 38 0 4 65 138 199 7.11e-11 56.6
MS.gene23262.t1 AT3G56860 38.710 62 38 0 4 65 138 199 7.11e-11 56.6
MS.gene23262.t1 AT3G56860 38.710 62 38 0 4 65 138 199 7.11e-11 56.6
MS.gene23262.t1 AT1G73530 31.944 72 49 0 5 76 76 147 8.17e-11 55.1

Find 20 sgRNAs with CRISPR-Local

Find 98 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACTGGACGATCAAGAGGTTT+TGG 0.283486 4.2:-36795941 MS.gene23262:CDS
ATCATTAACGATCGTGAAAC+TGG 0.286078 4.2:-36795959 MS.gene23262:CDS
AGCAATGAACGGTCAGGATT+TGG 0.313573 4.2:-36795873 MS.gene23262:CDS
TGCTTCAATGGCATCGTTCA+TGG 0.319166 4.2:+36795892 None:intergenic
CTGACCGTTCATTGCTTCAA+TGG 0.378604 4.2:+36795880 None:intergenic
TGATTGTTATTCTAGATGTT+GGG 0.408580 4.2:-36795554 MS.gene23262:intron
GCTTCGTCGGAGGTCTTGCA+TGG 0.475042 4.2:-36796377 MS.gene23262:CDS
CATTGAAGCAATGAACGGTC+AGG 0.516837 4.2:-36795879 MS.gene23262:CDS
CAAGACCTCCGACGAAGCAT+CGG 0.524569 4.2:+36796382 None:intergenic
GTTGAATACCGATGCTTCGT+CGG 0.525860 4.2:-36796390 MS.gene23262:CDS
CGGCGAGATCGTTGATTCCA+AGG 0.539852 4.2:-36796313 MS.gene23262:intron
GAGAAAGCCTTCTCTCAATA+CGG 0.543855 4.2:-36796333 MS.gene23262:CDS
TTTCTCGAGAGCATCATTGT+CGG 0.550717 4.2:+36796350 None:intergenic
CGTGAAACTGGACGATCAAG+AGG 0.551662 4.2:-36795947 MS.gene23262:CDS
GAATACCGATGCTTCGTCGG+AGG 0.590495 4.2:-36796387 MS.gene23262:CDS
CTCGAGAGCATCATTGTCGG+TGG 0.591544 4.2:+36796353 None:intergenic
CTTCGTCGGAGGTCTTGCAT+GGG 0.591814 4.2:-36796376 MS.gene23262:CDS
ATGAACGGTCAGGATTTGGA+TGG 0.621464 4.2:-36795869 MS.gene23262:CDS
GATCTCGCCGTATTGAGAGA+AGG 0.650761 4.2:+36796326 None:intergenic
GATGCCATTGAAGCAATGAA+CGG 0.670960 4.2:-36795884 MS.gene23262:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTTTCTTTTGATGTTTT+GGG - chr4.2:36795929-36795948 MS.gene23262:CDS 15.0%
!!! TTTTTTTTCTTTTGATGTTT+TGG - chr4.2:36795928-36795947 MS.gene23262:CDS 15.0%
!! ATGATTGTTATTCTAGATGT+TGG - chr4.2:36796392-36796411 MS.gene23262:CDS 25.0%
!! TGATTGTTATTCTAGATGTT+GGG - chr4.2:36796393-36796412 MS.gene23262:CDS 25.0%
AACAAAAGTTGAAGATCGAT+CGG - chr4.2:36795872-36795891 MS.gene23262:CDS 30.0%
!!! GATTTTGATTCGGATTTCTA+TGG - chr4.2:36795696-36795715 MS.gene23262:intron 30.0%
!!! TTTTGGGTTTTATTCTGATC+TGG - chr4.2:36795945-36795964 MS.gene23262:CDS 30.0%
AAGAAGATGAACGTACCTAT+TGG + chr4.2:36795846-36795865 None:intergenic 35.0%
AAGATCGATCGGTTACTATT+CGG - chr4.2:36795883-36795902 MS.gene23262:CDS 35.0%
ATCATTAACGATCGTGAAAC+TGG - chr4.2:36795988-36796007 MS.gene23262:intron 35.0%
TATTACTCACTCACACCAAT+AGG - chr4.2:36795828-36795847 MS.gene23262:intron 35.0%
!! ACTATTCGGTTCTAGATCTA+TGG - chr4.2:36795897-36795916 MS.gene23262:CDS 35.0%
!!! GATCTGACACGATTTTGATT+CGG - chr4.2:36795686-36795705 MS.gene23262:intron 35.0%
CAGAGTAACACAGAACAGAT+CGG + chr4.2:36795744-36795763 None:intergenic 40.0%
GAGAAAGCCTTCTCTCAATA+CGG - chr4.2:36795614-36795633 MS.gene23262:intron 40.0%
GATGCCATTGAAGCAATGAA+CGG - chr4.2:36796063-36796082 MS.gene23262:intron 40.0%
!! TTTCTCGAGAGCATCATTGT+CGG + chr4.2:36795600-36795619 None:intergenic 40.0%
AGCAATGAACGGTCAGGATT+TGG - chr4.2:36796074-36796093 MS.gene23262:intron 45.0%
ATGAACGGTCAGGATTTGGA+TGG - chr4.2:36796078-36796097 MS.gene23262:intron 45.0%
CATTGAAGCAATGAACGGTC+AGG - chr4.2:36796068-36796087 MS.gene23262:intron 45.0%
CTGACCGTTCATTGCTTCAA+TGG + chr4.2:36796070-36796089 None:intergenic 45.0%
GTTGAATACCGATGCTTCGT+CGG - chr4.2:36795557-36795576 MS.gene23262:intron 45.0%
TCGGAGATCACAGATCAGAA+AGG + chr4.2:36795725-36795744 None:intergenic 45.0%
TGCTTCAATGGCATCGTTCA+TGG + chr4.2:36796058-36796077 None:intergenic 45.0%
! ACTGGACGATCAAGAGGTTT+TGG - chr4.2:36796006-36796025 MS.gene23262:intron 45.0%
!! AGGAGGTGATGGTGGTTATT+AGG - chr4.2:36796350-36796369 MS.gene23262:CDS 45.0%
CGAAGCCTGCAAGCAACAAA+CGG + chr4.2:36795661-36795680 None:intergenic 50.0%
CGTGAAACTGGACGATCAAG+AGG - chr4.2:36796000-36796019 MS.gene23262:intron 50.0%
GATCTCGCCGTATTGAGAGA+AGG + chr4.2:36795624-36795643 None:intergenic 50.0%
TGCAAGCAACAAACGGACCT+TGG + chr4.2:36795654-36795673 None:intergenic 50.0%
! AAGGTCCGTTTGTTGCTTGC+AGG - chr4.2:36795653-36795672 MS.gene23262:intron 50.0%
! CGTTTGTTGCTTGCAGGCTT+CGG - chr4.2:36795659-36795678 MS.gene23262:intron 50.0%
CAAGACCTCCGACGAAGCAT+CGG + chr4.2:36795568-36795587 None:intergenic 55.0%
CTTCGTCGGAGGTCTTGCAT+GGG - chr4.2:36795571-36795590 MS.gene23262:intron 55.0%
GAATACCGATGCTTCGTCGG+AGG - chr4.2:36795560-36795579 MS.gene23262:intron 55.0%
GTTGCTTGCAGGCTTCGGAT+CGG - chr4.2:36795664-36795683 MS.gene23262:intron 55.0%
TACAGCAGAGGAGGAGGTGA+TGG - chr4.2:36796339-36796358 MS.gene23262:CDS 55.0%
TGTATCCACCACCTCCACCA+CGG + chr4.2:36796281-36796300 None:intergenic 55.0%
! CGTCGTGGATATGGTGGAAG+CGG - chr4.2:36796207-36796226 MS.gene23262:intron 55.0%
!! CGGCGAGATCGTTGATTCCA+AGG - chr4.2:36795634-36795653 MS.gene23262:intron 55.0%
!! CTCGAGAGCATCATTGTCGG+TGG + chr4.2:36795597-36795616 None:intergenic 55.0%
CAAGCTCAGAGCCGTGGTAG+CGG - chr4.2:36796120-36796139 MS.gene23262:intron 60.0%
CTCCATATCCACCGCCACCA+CGG + chr4.2:36796161-36796180 None:intergenic 60.0%
GCTTCGTCGGAGGTCTTGCA+TGG - chr4.2:36795570-36795589 MS.gene23262:intron 60.0%
GGTGGATACAGCAGAGGAGG+AGG - chr4.2:36796291-36796310 MS.gene23262:intron 60.0%
GGTGGATACAGCAGAGGAGG+CGG - chr4.2:36796291-36796310 MS.gene23262:intron 60.0%
GTGAACCAAGCTCAGAGCCG+TGG - chr4.2:36796114-36796133 MS.gene23262:intron 60.0%
! AGCAGAGGAGGAGGTGATGG+TGG - chr4.2:36796342-36796361 MS.gene23262:CDS 60.0%
! CGTGGATATGGTGGAAGCGG+TGG - chr4.2:36796210-36796229 MS.gene23262:intron 60.0%
! GGATATGGTGGAAGCGGTGG+TGG - chr4.2:36796213-36796232 MS.gene23262:intron 60.0%
! GGATATGGTGGAGGAGGTGG+TGG - chr4.2:36796234-36796253 MS.gene23262:intron 60.0%
! GGTGGATATGGAGGTGGTGG+TGG - chr4.2:36796168-36796187 MS.gene23262:intron 60.0%
! GGTGGATATGGTGGAGGAGG+TGG - chr4.2:36796231-36796250 MS.gene23262:intron 60.0%
!! AGCGGTGGTGGATATGGTGG+AGG - chr4.2:36796225-36796244 MS.gene23262:intron 60.0%
!! GGAAGCGGTGGTGGATATGG+TGG - chr4.2:36796222-36796241 MS.gene23262:intron 60.0%
!! GGAGAGCGTCGTGGATATGG+TGG - chr4.2:36796201-36796220 MS.gene23262:intron 60.0%
!! GGAGGAGGTGGTGGATATGG+TGG - chr4.2:36796243-36796262 MS.gene23262:intron 60.0%
!! GGAGGTGGTGGATACAGCAG+AGG - chr4.2:36796285-36796304 MS.gene23262:intron 60.0%
!! GGAGGTGGTGGATATGGTGG+TGG - chr4.2:36796246-36796265 MS.gene23262:intron 60.0%
!! GGAGGTGGTGGTGGTGGATA+TGG - chr4.2:36796177-36796196 MS.gene23262:intron 60.0%
!! GGATATGGAGGTGGTGGTGG+TGG - chr4.2:36796171-36796190 MS.gene23262:intron 60.0%
!! GGATATGGTGGAGAGCGTCG+TGG - chr4.2:36796192-36796211 MS.gene23262:intron 60.0%
!! GGTGGAAGCGGTGGTGGATA+TGG - chr4.2:36796219-36796238 MS.gene23262:intron 60.0%
!! GGTGGAGAGCGTCGTGGATA+TGG - chr4.2:36796198-36796217 MS.gene23262:intron 60.0%
!! GGTGGAGGAGGTGGTGGATA+TGG - chr4.2:36796240-36796259 MS.gene23262:intron 60.0%
!! GGTGGAGGATATGGTGGTGG+TGG - chr4.2:36796315-36796334 MS.gene23262:CDS 60.0%
!! GGTGGTGGAGGATATGGTGG+TGG - chr4.2:36796312-36796331 MS.gene23262:intron 60.0%
!! GGTGGTGGATACAGCAGAGG+AGG - chr4.2:36796288-36796307 MS.gene23262:intron 60.0%
!! GGTGGTGGATATGGTGGAGG+AGG - chr4.2:36796228-36796247 MS.gene23262:intron 60.0%
!! GGTGGTGGATATGGTGGTGG+TGG - chr4.2:36796249-36796268 MS.gene23262:intron 60.0%
!! GGTGGTGGTGGATACAGCAG+AGG - chr4.2:36796327-36796346 MS.gene23262:CDS 60.0%
!! GGTGGTGGTGGTGGATATGG+TGG - chr4.2:36796180-36796199 MS.gene23262:intron 60.0%
ATCCACCACCTCCACCACGG+CGG + chr4.2:36796278-36796297 None:intergenic 65.0%
GGATACAGCAGAGGAGGCGG+TGG - chr4.2:36796294-36796313 MS.gene23262:intron 65.0%
GGCGGTGGATATGGAGGTGG+TGG - chr4.2:36796165-36796184 MS.gene23262:intron 65.0%
TACAGCAGAGGAGGCGGTGG+TGG - chr4.2:36796297-36796316 MS.gene23262:intron 65.0%
! GGTGGCGGTGGATATGGAGG+TGG - chr4.2:36796162-36796181 MS.gene23262:intron 65.0%
!! CGCTACCACGGCTCTGAGCT+TGG + chr4.2:36796122-36796141 None:intergenic 65.0%
!! CGTGGTGGCGGTGGATATGG+AGG - chr4.2:36796159-36796178 MS.gene23262:intron 65.0%
!! GGAGGCGGTGGTGGAGGATA+TGG - chr4.2:36796306-36796325 MS.gene23262:intron 65.0%
!! GGCGGTGGTGGAGGATATGG+TGG - chr4.2:36796309-36796328 MS.gene23262:intron 65.0%
CTCCGCCACCACCGCTACCA+CGG + chr4.2:36796134-36796153 None:intergenic 70.0%
! AGCAGAGGAGGCGGTGGTGG+AGG - chr4.2:36796300-36796319 MS.gene23262:intron 70.0%
!! AGCCGTGGTAGCGGTGGTGG+CGG - chr4.2:36796129-36796148 MS.gene23262:intron 70.0%
!! CAGAGCCGTGGTAGCGGTGG+TGG - chr4.2:36796126-36796145 MS.gene23262:intron 70.0%
!! GCTCAGAGCCGTGGTAGCGG+TGG - chr4.2:36796123-36796142 MS.gene23262:intron 70.0%
!! GGCCGTGGTGGCGGTGGATA+TGG - chr4.2:36796156-36796175 MS.gene23262:intron 70.0%
GGTGGTGACCGCCGTGGTGG+AGG - chr4.2:36796267-36796286 MS.gene23262:intron 75.0%
! GACCGCCGTGGTGGAGGTGG+TGG - chr4.2:36796273-36796292 MS.gene23262:intron 75.0%
! GGTGACCGCCGTGGTGGAGG+TGG - chr4.2:36796270-36796289 MS.gene23262:intron 75.0%
! GGTGGTGGTGACCGCCGTGG+TGG - chr4.2:36796264-36796283 MS.gene23262:intron 75.0%
!! CGTGGTAGCGGTGGTGGCGG+AGG - chr4.2:36796132-36796151 MS.gene23262:intron 75.0%
!! GGTAGCGGTGGTGGCGGAGG+AGG - chr4.2:36796135-36796154 MS.gene23262:intron 75.0%
!! GGTGGTGGTGGTGACCGCCG+TGG - chr4.2:36796261-36796280 MS.gene23262:intron 75.0%
! GGCGGAGGAGGCCGTGGTGG+CGG - chr4.2:36796147-36796166 MS.gene23262:intron 80.0%
!!! GGAGGAGGCCGTGGTGGCGG+TGG - chr4.2:36796150-36796169 MS.gene23262:intron 80.0%
!!! GGTGGCGGAGGAGGCCGTGG+TGG - chr4.2:36796144-36796163 MS.gene23262:intron 80.0%
!!! GGTGGTGGCGGAGGAGGCCG+TGG - chr4.2:36796141-36796160 MS.gene23262:intron 80.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 36795548 36796421 36795548 ID=MS.gene23262
chr4.2 mRNA 36795548 36796421 36795548 ID=MS.gene23262.t1;Parent=MS.gene23262
chr4.2 exon 36796314 36796421 36796314 ID=MS.gene23262.t1.exon1;Parent=MS.gene23262.t1
chr4.2 CDS 36796314 36796421 36796314 ID=cds.MS.gene23262.t1;Parent=MS.gene23262.t1
chr4.2 exon 36795867 36795981 36795867 ID=MS.gene23262.t1.exon2;Parent=MS.gene23262.t1
chr4.2 CDS 36795867 36795981 36795867 ID=cds.MS.gene23262.t1;Parent=MS.gene23262.t1
chr4.2 exon 36795548 36795561 36795548 ID=MS.gene23262.t1.exon3;Parent=MS.gene23262.t1
chr4.2 CDS 36795548 36795561 36795548 ID=cds.MS.gene23262.t1;Parent=MS.gene23262.t1
Gene Sequence

>MS.gene23262

ATGGCTGATGTTGAATACCGATGCTTCGTCGGAGGTCTTGCATGGGCCACCGACAATGATGCTCTCGAGAAAGCCTTCTCTCAATACGGCGAGATCGTTGATTCCAAGGTCCGTTTGTTGCTTGCAGGCTTCGGATCGGATCTGACACGATTTTGATTCGGATTTCTATGGAAACCTTTCTGATCTGTGATCTCCGATCTGTTCTGTGTTACTCTGTTACTTGATCTATTTGTTACTGTTACTGTGATTCTGATTCTGTTACTGTATGATTTGATTCTTTTATTACTCACTCACACCAATAGGTACGTTCATCTTCTTCTTTTGAACAAAAGTTGAAGATCGATCGGTTACTATTCGGTTCTAGATCTATGGATCTGATTTTTTTTTTCTTTTGATGTTTTGGGTTTTATTCTGATCTGGTTTGTTAATTTTTTGTGCAGATCATTAACGATCGTGAAACTGGACGATCAAGAGGTTTTGGATTTGTGACATTCGCTAACGAGAAATCCATGAACGATGCCATTGAAGCAATGAACGGTCAGGATTTGGATGGACGTAACATCACTGTGAACCAAGCTCAGAGCCGTGGTAGCGGTGGTGGCGGAGGAGGCCGTGGTGGCGGTGGATATGGAGGTGGTGGTGGTGGATATGGTGGAGAGCGTCGTGGATATGGTGGAAGCGGTGGTGGATATGGTGGAGGAGGTGGTGGATATGGTGGTGGTGGTGACCGCCGTGGTGGAGGTGGTGGATACAGCAGAGGAGGCGGTGGTGGAGGATATGGTGGTGGTGGATACAGCAGAGGAGGAGGTGATGGTGGTTATTAGGTTTTAGATGTTTATCGTTTATGATTGTTATTCTAGATGTTGGGATCTAG
Protein sequence

>MS.gene23262.t1

MADVEYRCFVGGLAWATDNDALEKAFSQYGEIVDSKIINDRETGRSRGFGFVTFANEKSMNDAIEAMNGQDLDGHVGI