Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene23264.t1 | RHN61292.1 | 97.4 | 39 | 1 | 0 | 1 | 39 | 1 | 39 | 8.50E-13 | 82 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene23264.t1 | P49310 | 82.1 | 39 | 7 | 0 | 1 | 39 | 1 | 39 | 9.5e-13 | 73.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene23264.t1 | G7JHA8 | 97.4 | 39 | 1 | 0 | 1 | 39 | 1 | 39 | 6.1e-13 | 82.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050702 | MS.gene23264 | -0.884672 | 1.57E-71 | -1.69E-46 |
| MS.gene051678 | MS.gene23264 | 0.82459 | 7.43E-54 | -1.69E-46 |
| MS.gene052224 | MS.gene23264 | -0.840504 | 8.36E-58 | -1.69E-46 |
| MS.gene053294 | MS.gene23264 | 0.809149 | 2.18E-50 | -1.69E-46 |
| MS.gene053401 | MS.gene23264 | 0.927221 | 1.59E-91 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene23264.t1 | MTR_4g070190 | 97.436 | 39 | 1 | 0 | 1 | 39 | 1 | 39 | 8.42e-22 | 82.0 |
| MS.gene23264.t1 | MTR_4g070080 | 91.667 | 36 | 3 | 0 | 4 | 39 | 2 | 37 | 7.77e-20 | 75.9 |
| MS.gene23264.t1 | MTR_4g070080 | 91.667 | 36 | 3 | 0 | 4 | 39 | 2 | 37 | 1.92e-19 | 75.9 |
| MS.gene23264.t1 | MTR_4g070140 | 82.051 | 39 | 7 | 0 | 1 | 39 | 1 | 39 | 5.33e-18 | 72.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene23264.t1 | AT2G21660 | 79.487 | 39 | 8 | 0 | 1 | 39 | 1 | 39 | 1.11e-17 | 71.2 |
| MS.gene23264.t1 | AT2G21660 | 79.487 | 39 | 8 | 0 | 1 | 39 | 1 | 39 | 1.56e-17 | 71.2 |
| MS.gene23264.t1 | AT4G39260 | 64.865 | 37 | 13 | 0 | 4 | 40 | 2 | 38 | 2.33e-14 | 61.2 |
| MS.gene23264.t1 | AT4G39260 | 66.667 | 36 | 12 | 0 | 4 | 39 | 2 | 37 | 3.63e-14 | 60.8 |
| MS.gene23264.t1 | AT4G39260 | 64.865 | 37 | 13 | 0 | 4 | 40 | 2 | 38 | 4.80e-14 | 61.2 |
| MS.gene23264.t1 | AT4G39260 | 64.865 | 37 | 13 | 0 | 4 | 40 | 2 | 38 | 1.11e-13 | 61.2 |
Find 13 sgRNAs with CRISPR-Local
Find 13 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGTCTGTTTGTGTTGCTTGC+AGG | 0.295171 | 4.2:+36610794 | MS.gene23264:CDS |
| TTTGTGTTGCTTGCAGGCTT+CGG | 0.390810 | 4.2:+36610800 | MS.gene23264:CDS |
| GCTTCGTCGGAGGTCTTGCA+TGG | 0.446995 | 4.2:+36610709 | MS.gene23264:CDS |
| GTTGAGTACCGTTGCTTCGT+CGG | 0.485717 | 4.2:+36610696 | MS.gene23264:CDS |
| GAGAAAGCCTTCTCTCAATA+CGG | 0.531059 | 4.2:+36610753 | MS.gene23264:CDS |
| GTTGCTTGCAGGCTTCGGAT+CGG | 0.542597 | 4.2:+36610805 | MS.gene23264:CDS |
| CTTCGTCGGAGGTCTTGCAT+GGG | 0.553297 | 4.2:+36610710 | MS.gene23264:CDS |
| CGGAGAAATCATCGATTCGA+AGG | 0.564623 | 4.2:+36610773 | MS.gene23264:CDS |
| CTCGAGAGCTTCGCTATCGG+TGG | 0.564705 | 4.2:-36610733 | None:intergenic |
| GATTTCTCCGTATTGAGAGA+AGG | 0.569467 | 4.2:-36610760 | None:intergenic |
| TTTCTCGAGAGCTTCGCTAT+CGG | 0.583736 | 4.2:-36610736 | None:intergenic |
| CAAGACCTCCGACGAAGCAA+CGG | 0.604652 | 4.2:-36610704 | None:intergenic |
| GAGTACCGTTGCTTCGTCGG+AGG | 0.615538 | 4.2:+36610699 | MS.gene23264:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| GAGAAAGCCTTCTCTCAATA+CGG | + | chr4.2:36610753-36610772 | MS.gene23264:CDS | 40.0% | |
| GATTTCTCCGTATTGAGAGA+AGG | - | chr4.2:36610763-36610782 | None:intergenic | 40.0% | |
| TTTCTCGAGAGCTTCGCTAT+CGG | - | chr4.2:36610739-36610758 | None:intergenic | 45.0% | |
| !! | CGGAGAAATCATCGATTCGA+AGG | + | chr4.2:36610773-36610792 | MS.gene23264:CDS | 45.0% |
| !! | TTTGTGTTGCTTGCAGGCTT+CGG | + | chr4.2:36610800-36610819 | MS.gene23264:CDS | 45.0% |
| ! | GTTGAGTACCGTTGCTTCGT+CGG | + | chr4.2:36610696-36610715 | MS.gene23264:CDS | 50.0% |
| !! | GGTCTGTTTGTGTTGCTTGC+AGG | + | chr4.2:36610794-36610813 | MS.gene23264:CDS | 50.0% |
| CAAGACCTCCGACGAAGCAA+CGG | - | chr4.2:36610707-36610726 | None:intergenic | 55.0% | |
| CTTCGTCGGAGGTCTTGCAT+GGG | + | chr4.2:36610710-36610729 | MS.gene23264:CDS | 55.0% | |
| GTTGCTTGCAGGCTTCGGAT+CGG | + | chr4.2:36610805-36610824 | MS.gene23264:CDS | 55.0% | |
| CTCGAGAGCTTCGCTATCGG+TGG | - | chr4.2:36610736-36610755 | None:intergenic | 60.0% | |
| GCTTCGTCGGAGGTCTTGCA+TGG | + | chr4.2:36610709-36610728 | MS.gene23264:CDS | 60.0% | |
| ! | GAGTACCGTTGCTTCGTCGG+AGG | + | chr4.2:36610699-36610718 | MS.gene23264:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.2 | gene | 36610681 | 36610833 | 36610681 | ID=MS.gene23264 |
| chr4.2 | mRNA | 36610681 | 36610833 | 36610681 | ID=MS.gene23264.t1;Parent=MS.gene23264 |
| chr4.2 | exon | 36610681 | 36610833 | 36610681 | ID=MS.gene23264.t1.exon1;Parent=MS.gene23264.t1 |
| chr4.2 | CDS | 36610681 | 36610833 | 36610681 | ID=cds.MS.gene23264.t1;Parent=MS.gene23264.t1 |
| Gene Sequence |
| Protein sequence |
