Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene23490.t1 | XP_013449892.1 | 95.1 | 536 | 26 | 0 | 1 | 536 | 1 | 536 | 4.00E-294 | 1020 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene23490.t1 | Q96303 | 82.0 | 527 | 92 | 1 | 1 | 524 | 1 | 527 | 3.7e-256 | 885.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene23490.t1 | A0A072UDN5 | 95.1 | 536 | 26 | 0 | 1 | 536 | 1 | 536 | 2.9e-294 | 1020.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene067567 | MS.gene23490 | PPI |
| MS.gene059428 | MS.gene23490 | PPI |
| MS.gene56432 | MS.gene23490 | PPI |
| MS.gene006892 | MS.gene23490 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene23490.t1 | MTR_7g096870 | 97.575 | 536 | 13 | 0 | 1 | 536 | 1 | 536 | 0.0 | 1084 |
| MS.gene23490.t1 | MTR_7g096880 | 90.637 | 534 | 48 | 1 | 1 | 534 | 1 | 532 | 0.0 | 962 |
| MS.gene23490.t1 | MTR_1g069935 | 85.356 | 519 | 76 | 0 | 1 | 519 | 1 | 519 | 0.0 | 941 |
| MS.gene23490.t1 | MTR_3g082700 | 75.741 | 540 | 128 | 1 | 1 | 537 | 1 | 540 | 0.0 | 837 |
| MS.gene23490.t1 | MTR_1g069930 | 75.340 | 515 | 126 | 1 | 1 | 514 | 1 | 515 | 0.0 | 835 |
| MS.gene23490.t1 | MTR_1g074930 | 76.834 | 518 | 116 | 2 | 5 | 518 | 4 | 521 | 0.0 | 833 |
| MS.gene23490.t1 | MTR_1g043220 | 75.376 | 532 | 127 | 2 | 5 | 532 | 4 | 535 | 0.0 | 822 |
| MS.gene23490.t1 | MTR_1g043200 | 76.281 | 527 | 119 | 3 | 5 | 527 | 4 | 528 | 0.0 | 817 |
| MS.gene23490.t1 | MTR_1g074940 | 76.654 | 514 | 100 | 4 | 1 | 508 | 1 | 500 | 0.0 | 813 |
| MS.gene23490.t1 | MTR_1g043290 | 75.475 | 526 | 125 | 2 | 5 | 526 | 4 | 529 | 0.0 | 812 |
| MS.gene23490.t1 | MTR_1g028600 | 61.089 | 514 | 188 | 3 | 6 | 518 | 3 | 505 | 0.0 | 634 |
| MS.gene23490.t1 | MTR_5g068140 | 57.537 | 544 | 203 | 4 | 8 | 532 | 7 | 541 | 0.0 | 619 |
| MS.gene23490.t1 | MTR_4g083960 | 51.866 | 536 | 227 | 12 | 6 | 534 | 5 | 516 | 0.0 | 518 |
| MS.gene23490.t1 | MTR_3g084110 | 21.875 | 448 | 288 | 15 | 76 | 512 | 76 | 472 | 1.80e-13 | 73.2 |
| MS.gene23490.t1 | MTR_7g446340 | 23.340 | 467 | 293 | 17 | 76 | 527 | 104 | 520 | 3.33e-12 | 69.3 |
| MS.gene23490.t1 | MTR_6g007340 | 23.340 | 467 | 293 | 17 | 76 | 527 | 104 | 520 | 3.33e-12 | 69.3 |
| MS.gene23490.t1 | MTR_6g088450 | 25.564 | 399 | 245 | 12 | 81 | 463 | 108 | 470 | 4.93e-12 | 68.6 |
| MS.gene23490.t1 | MTR_1g116660 | 21.429 | 434 | 277 | 14 | 90 | 511 | 97 | 478 | 5.07e-12 | 68.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene23490.t1 | AT2G38940 | 85.078 | 516 | 74 | 1 | 1 | 513 | 1 | 516 | 0.0 | 928 |
| MS.gene23490.t1 | AT3G54700 | 84.556 | 518 | 79 | 1 | 1 | 518 | 1 | 517 | 0.0 | 926 |
| MS.gene23490.t1 | AT3G54700 | 84.556 | 518 | 79 | 1 | 1 | 518 | 1 | 517 | 0.0 | 926 |
| MS.gene23490.t1 | AT2G32830 | 74.122 | 541 | 136 | 1 | 1 | 537 | 1 | 541 | 0.0 | 853 |
| MS.gene23490.t1 | AT5G43360 | 78.077 | 520 | 112 | 1 | 1 | 518 | 1 | 520 | 0.0 | 839 |
| MS.gene23490.t1 | AT5G43350 | 77.992 | 518 | 112 | 1 | 1 | 516 | 1 | 518 | 0.0 | 837 |
| MS.gene23490.t1 | AT5G43370 | 77.799 | 518 | 113 | 1 | 1 | 516 | 1 | 518 | 0.0 | 808 |
| MS.gene23490.t1 | AT5G43370 | 77.799 | 518 | 113 | 1 | 1 | 516 | 1 | 518 | 0.0 | 808 |
| MS.gene23490.t1 | AT5G43340 | 67.181 | 518 | 155 | 2 | 3 | 518 | 4 | 508 | 0.0 | 737 |
| MS.gene23490.t1 | AT1G20860 | 48.972 | 535 | 255 | 9 | 6 | 533 | 3 | 526 | 7.72e-174 | 501 |
| MS.gene23490.t1 | AT1G76430 | 47.858 | 537 | 251 | 11 | 5 | 528 | 3 | 523 | 1.67e-173 | 501 |
| MS.gene23490.t1 | AT1G20860 | 48.972 | 535 | 255 | 9 | 6 | 533 | 55 | 578 | 4.69e-173 | 501 |
| MS.gene23490.t1 | AT4G08878 | 68.487 | 238 | 75 | 0 | 31 | 268 | 13 | 250 | 3.50e-115 | 342 |
| MS.gene23490.t1 | AT4G08895 | 55.944 | 143 | 31 | 1 | 318 | 460 | 23 | 133 | 5.96e-47 | 161 |
| MS.gene23490.t1 | AT2G43330 | 22.748 | 444 | 260 | 17 | 90 | 511 | 93 | 475 | 2.02e-11 | 67.0 |
Find 138 sgRNAs with CRISPR-Local
Find 161 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACCTTCTTCTTTGCAAATTT+TGG | -0.031518 | 7.3:-14517955 | MS.gene23490:CDS |
| CCCTTTGCTTCTTCCGGTTT+TGG | 0.142326 | 7.3:-14518797 | MS.gene23490:CDS |
| TATCCTTCTGGAAGAGATTT+TGG | 0.150755 | 7.3:+14518250 | None:intergenic |
| GCTTTCTGTGGAACACTTTC+AGG | 0.150980 | 7.3:-14518954 | MS.gene23490:CDS |
| CATGCAGGGTTTCGGAATTT+TGG | 0.151969 | 7.3:-14518673 | MS.gene23490:CDS |
| GTTGGGATGTTGTAACATTT+TGG | 0.179506 | 7.3:-14517728 | MS.gene23490:CDS |
| TGCATTCGGATTCTTGTATT+TGG | 0.230467 | 7.3:-14517824 | MS.gene23490:CDS |
| GCGGTATAACGAGCAGTTTC+TGG | 0.246126 | 7.3:+14518474 | None:intergenic |
| GTTATCTGTTCAATTGGTTC+TGG | 0.268162 | 7.3:-14518858 | MS.gene23490:CDS |
| AGGGTTGTCATGACAGATTT+TGG | 0.291172 | 7.3:+14518816 | None:intergenic |
| ATTGGTTCTGGTCTTTCTTT+CGG | 0.310334 | 7.3:-14518846 | MS.gene23490:CDS |
| AACCTCCTCAAGTGCATTCA+TGG | 0.318200 | 7.3:+14518191 | None:intergenic |
| TGACAACCCTTTGCTTCTTC+CGG | 0.331448 | 7.3:-14518803 | MS.gene23490:CDS |
| ATTAGAATACTCCGACATTA+TGG | 0.331666 | 7.3:+14518734 | None:intergenic |
| CTTCTCCTCTTTCTGTGGTT+CGG | 0.335668 | 7.3:+14518383 | None:intergenic |
| CATAATTTCATCTGCATTCA+AGG | 0.338184 | 7.3:-14518631 | MS.gene23490:CDS |
| GTCTTTGCCATGCAGGGTTT+CGG | 0.339550 | 7.3:-14518681 | MS.gene23490:CDS |
| TTGCACTGAAAATATCCTTC+TGG | 0.362845 | 7.3:+14518238 | None:intergenic |
| TTCCCCAAGCAGACTACATT+TGG | 0.372520 | 7.3:-14518560 | MS.gene23490:CDS |
| CCTCCAAATGTAGTCTGCTT+GGG | 0.372673 | 7.3:+14518557 | None:intergenic |
| GCAGTACAGTACCAGGCTAT+TGG | 0.376413 | 7.3:-14518131 | MS.gene23490:CDS |
| AAGATTTACCATCCAATTGA+TGG | 0.386978 | 7.3:-14518079 | MS.gene23490:CDS |
| TGGTGGTGACTACCCTCTTT+CGG | 0.394304 | 7.3:-14518760 | MS.gene23490:CDS |
| TGCAGCAGTCTTTGCCATGC+AGG | 0.397867 | 7.3:-14518688 | MS.gene23490:CDS |
| ACAACAAATGTTGTAGCATT+AGG | 0.400761 | 7.3:+14517931 | None:intergenic |
| TAAAGAAGAATCCCATCAAT+TGG | 0.402453 | 7.3:+14518067 | None:intergenic |
| CATCCGCATCGGGAAAGCTT+GGG | 0.403184 | 7.3:-14517858 | MS.gene23490:CDS |
| GGGGCTATAGTTGGTGCATT+CGG | 0.412118 | 7.3:-14517838 | MS.gene23490:CDS |
| TAGCACCTCAATTTGATCCT+TGG | 0.413690 | 7.3:+14519178 | None:intergenic |
| TCCTCCAAATGTAGTCTGCT+TGG | 0.420387 | 7.3:+14518556 | None:intergenic |
| CGCATATACTACCACGTTGA+TGG | 0.426932 | 7.3:-14519032 | MS.gene23490:CDS |
| TACCGAACCCAAGCCAAAAC+CGG | 0.427494 | 7.3:+14518784 | None:intergenic |
| TGGCTGGCTTGGTGATAAGA+TGG | 0.431799 | 7.3:-14518919 | MS.gene23490:CDS |
| GGTGAGAATGATGAGGAAGT+TGG | 0.432793 | 7.3:-14517646 | MS.gene23490:CDS |
| GGCAAATGGAAAATCTTTAG+AGG | 0.436138 | 7.3:-14517677 | MS.gene23490:CDS |
| GCAGGGTACCCTGCAGGTAT+TGG | 0.442599 | 7.3:-14517772 | MS.gene23490:CDS |
| ATGATGGTTATCTGTTCAAT+TGG | 0.447387 | 7.3:-14518864 | MS.gene23490:CDS |
| GGCAAAGACTGCTGCAATGA+AGG | 0.448745 | 7.3:+14518695 | None:intergenic |
| TGCAATCATCATAGCTGGTA+TGG | 0.448896 | 7.3:-14519114 | MS.gene23490:CDS |
| AGCTGCAGCAGATATGTCTA+AGG | 0.462417 | 7.3:-14518427 | MS.gene23490:CDS |
| TTATGAGTCCAGTGTTCATA+AGG | 0.464297 | 7.3:+14518015 | None:intergenic |
| GGCTGGCTTGGTGATAAGAT+GGG | 0.464364 | 7.3:-14518918 | MS.gene23490:CDS |
| TCATCCGCATCGGGAAAGCT+TGG | 0.465982 | 7.3:-14517859 | MS.gene23490:CDS |
| GACAAGAGTAAAGCAGATGC+AGG | 0.466575 | 7.3:-14517790 | MS.gene23490:CDS |
| AAGGAAAAGGCTAAATCATA+TGG | 0.468204 | 7.3:-14518363 | MS.gene23490:CDS |
| GAGTAGTAAGTCATTGCAGC+TGG | 0.469391 | 7.3:+14518510 | None:intergenic |
| TGGGGAACAGTTGAACCAAC+TGG | 0.470918 | 7.3:+14518576 | None:intergenic |
| ACATCACCACAAATCCTATT+CGG | 0.472436 | 7.3:+14517986 | None:intergenic |
| ATTCACAGCAGCTGATACAT+TGG | 0.472564 | 7.3:+14518983 | None:intergenic |
| TTCTTAGTACCCGAGGCAAA+TGG | 0.472965 | 7.3:-14517691 | MS.gene23490:CDS |
| GTGAATGGTGTTGCTTTCTG+TGG | 0.474993 | 7.3:-14518966 | MS.gene23490:CDS |
| ACTCATAAGGATAACCGAAT+AGG | 0.475963 | 7.3:-14518000 | MS.gene23490:CDS |
| TGAAGCCGAACCACAGAAAG+AGG | 0.480495 | 7.3:-14518388 | MS.gene23490:CDS |
| GACATTATGGTAGCCGAAAG+AGG | 0.481182 | 7.3:+14518747 | None:intergenic |
| TTTCATCTGCATTCAAGGCC+AGG | 0.481632 | 7.3:-14518626 | MS.gene23490:CDS |
| CTTGTATTTGGCACAAAACA+AGG | 0.489073 | 7.3:-14517812 | MS.gene23490:CDS |
| TGGCTTGGGTTCGGTATTGG+TGG | 0.492801 | 7.3:-14518777 | MS.gene23490:CDS |
| TCCCTTGTTACCAAGTTGCT+CGG | 0.496149 | 7.3:-14519056 | MS.gene23490:CDS |
| TTATGAACACTGGACTCATA+AGG | 0.499390 | 7.3:-14518013 | MS.gene23490:CDS |
| CCCAAAATTTGCAAAGAAGA+AGG | 0.507277 | 7.3:+14517954 | None:intergenic |
| CCCAAGCAGACTACATTTGG+AGG | 0.513096 | 7.3:-14518557 | MS.gene23490:CDS |
| AGTGCATTCATGGTTTGTGC+AGG | 0.513709 | 7.3:+14518201 | None:intergenic |
| GGAAAGCTTGGGGCTATAGT+TGG | 0.514607 | 7.3:-14517847 | MS.gene23490:CDS |
| GCAGCAGTCTTTGCCATGCA+GGG | 0.514618 | 7.3:-14518687 | MS.gene23490:CDS |
| AGATTTACCATCCAATTGAT+GGG | 0.514693 | 7.3:-14518078 | MS.gene23490:CDS |
| CAAGTTGATCGAAATCTAGC+TGG | 0.515101 | 7.3:+14517892 | None:intergenic |
| TTCACTGCAATCATCATAGC+TGG | 0.515551 | 7.3:-14519119 | MS.gene23490:CDS |
| ACAGAAAGAGGAGAAGGAAA+AGG | 0.518948 | 7.3:-14518376 | MS.gene23490:CDS |
| GGATAACCGAATAGGATTTG+TGG | 0.523531 | 7.3:-14517992 | MS.gene23490:CDS |
| CATGGAATATCATCCGCATC+GGG | 0.524513 | 7.3:-14517868 | MS.gene23490:CDS |
| GGCTTGGTGATAAGATGGGC+AGG | 0.528400 | 7.3:-14518914 | MS.gene23490:CDS |
| GCCAAAACCGGAAGAAGCAA+AGG | 0.528845 | 7.3:+14518796 | None:intergenic |
| CTATGATGATTGCAGTGAAA+TGG | 0.528943 | 7.3:+14519123 | None:intergenic |
| GCATTCATGGTTTGTGCAGG+AGG | 0.529180 | 7.3:+14518204 | None:intergenic |
| TTCTTCATACCAATACCTGC+AGG | 0.529263 | 7.3:+14517763 | None:intergenic |
| CGAACCACAGAAAGAGGAGA+AGG | 0.530308 | 7.3:-14518382 | MS.gene23490:CDS |
| TTCACAGCAGCTGATACATT+GGG | 0.530334 | 7.3:+14518984 | None:intergenic |
| CGTCATGGACTACATTTGCT+TGG | 0.532151 | 7.3:-14518315 | MS.gene23490:CDS |
| GTGGCTTTCATAGACAGAAT+AGG | 0.532759 | 7.3:-14518102 | MS.gene23490:CDS |
| TACATTGGGAGGCAACGTAC+CGG | 0.539000 | 7.3:+14518998 | None:intergenic |
| AGATTTCGATCAACTTGCCA+TGG | 0.539975 | 7.3:-14517886 | MS.gene23490:CDS |
| AATGTATCAGCTGCTGTGAA+TGG | 0.543645 | 7.3:-14518981 | MS.gene23490:CDS |
| GCAGATGCAGGGTACCCTGC+AGG | 0.546146 | 7.3:-14517778 | MS.gene23490:CDS |
| GTTGATGGTGCAGCAAAGCC+CGG | 0.548274 | 7.3:-14519017 | MS.gene23490:CDS |
| GGCTTGAATAGTCATGGCCA+AGG | 0.551613 | 7.3:-14519195 | None:intergenic |
| AAATCTTTAGAGGAAATGTC+AGG | 0.557207 | 7.3:-14517667 | MS.gene23490:CDS |
| TTGCCATTCCTTATGAACAC+TGG | 0.558026 | 7.3:-14518023 | MS.gene23490:CDS |
| CCATGGAATATCATCCGCAT+CGG | 0.559707 | 7.3:-14517869 | MS.gene23490:CDS |
| TCTTACGAGGTTGATCCAGT+TGG | 0.560435 | 7.3:-14518591 | MS.gene23490:CDS |
| ACATTGGGAGGCAACGTACC+GGG | 0.562466 | 7.3:+14518999 | None:intergenic |
| TGGAGGATAATTGTGATGGT+AGG | 0.563401 | 7.3:-14518540 | MS.gene23490:CDS |
| AGTCCAGTGTTCATAAGGAA+TGG | 0.563480 | 7.3:+14518020 | None:intergenic |
| GACCGAGCAACTTGGTAACA+AGG | 0.566465 | 7.3:+14519054 | None:intergenic |
| TGCTTGGTACAACAAGCACT+TGG | 0.567808 | 7.3:-14518299 | MS.gene23490:CDS |
| GCAATCATCATAGCTGGTAT+GGG | 0.570874 | 7.3:-14519113 | MS.gene23490:CDS |
| GAAGAATCCCATCAATTGGA+TGG | 0.573451 | 7.3:+14518071 | None:intergenic |
| CATTCATGGTTTGTGCAGGA+GGG | 0.580236 | 7.3:+14518205 | None:intergenic |
| TAGCCAAAATCTCTTCCAGA+AGG | 0.581352 | 7.3:-14518253 | MS.gene23490:CDS |
| GCCACGGTAAACCAATAGCC+TGG | 0.581737 | 7.3:+14518120 | None:intergenic |
| GCTCTATGCAGTACAGTACC+AGG | 0.583363 | 7.3:-14518138 | MS.gene23490:CDS |
| AAAGAGTTCATGAGTCGTCA+TGG | 0.583437 | 7.3:-14518330 | MS.gene23490:CDS |
| TGGTATGACCCTCATGATGA+TGG | 0.586953 | 7.3:-14518880 | MS.gene23490:CDS |
| AACCATGAATGCACTTGAGG+AGG | 0.589777 | 7.3:-14518193 | MS.gene23490:CDS |
| CCGATGCGGATGATATTCCA+TGG | 0.591183 | 7.3:+14517869 | None:intergenic |
| CATGGCCAAGGATCAAATTG+AGG | 0.592004 | 7.3:-14519183 | MS.gene23490:CDS |
| CCAAAACCGGAAGAAGCAAA+GGG | 0.592671 | 7.3:+14518797 | None:intergenic |
| AGCCCCAAGCTTTCCCGATG+CGG | 0.596062 | 7.3:+14517855 | None:intergenic |
| CTCCAAATGTAGTCTGCTTG+GGG | 0.596396 | 7.3:+14518558 | None:intergenic |
| ATCCGCATCGGGAAAGCTTG+GGG | 0.597584 | 7.3:-14517857 | MS.gene23490:CDS |
| AATGTCAGGTGAGAATGATG+AGG | 0.605492 | 7.3:-14517653 | MS.gene23490:CDS |
| CATTTGGAGGATAATTGTGA+TGG | 0.611828 | 7.3:-14518544 | MS.gene23490:CDS |
| GCGTTGTTATTCTGAGCATG+AGG | 0.612504 | 7.3:+14517601 | None:intergenic |
| GTTTGATTCTCCGTCTTACG+AGG | 0.613183 | 7.3:-14518604 | MS.gene23490:CDS |
| ACTGGATCAACCTCGTAAGA+CGG | 0.613448 | 7.3:+14518594 | None:intergenic |
| ACAAACCATGAATGCACTTG+AGG | 0.613620 | 7.3:-14518196 | MS.gene23490:CDS |
| AGTAGTAAGTCATTGCAGCT+GGG | 0.615517 | 7.3:+14518511 | None:intergenic |
| AACAGATAACCATCATCATG+AGG | 0.618082 | 7.3:+14518871 | None:intergenic |
| GTATATGCGACCGAGCAACT+TGG | 0.619928 | 7.3:+14519046 | None:intergenic |
| ACATTATGGTAGCCGAAAGA+GGG | 0.623125 | 7.3:+14518748 | None:intergenic |
| ACCAGGCTATTGGTTTACCG+TGG | 0.630346 | 7.3:-14518121 | MS.gene23490:CDS |
| TATTCTGTCTATGAAAGCCA+CGG | 0.630634 | 7.3:+14518104 | None:intergenic |
| GGTGCTAAATGCGCTTGATG+TGG | 0.631666 | 7.3:-14519162 | MS.gene23490:CDS |
| ATTTGCAAAGAAGAAGGTCA+AGG | 0.633508 | 7.3:+14517960 | None:intergenic |
| GTGCACTTTCTTAGTACCCG+AGG | 0.637975 | 7.3:-14517698 | MS.gene23490:CDS |
| AATGGCAAGAGCAAACATGA+AGG | 0.639251 | 7.3:+14518038 | None:intergenic |
| CAAAATTCCGAAACCCTGCA+TGG | 0.639934 | 7.3:+14518674 | None:intergenic |
| ACAGCAGCTGATACATTGGG+AGG | 0.653095 | 7.3:+14518987 | None:intergenic |
| ACCGAGCAACTTGGTAACAA+GGG | 0.653584 | 7.3:+14519055 | None:intergenic |
| ACAAGAGTAAAGCAGATGCA+GGG | 0.657371 | 7.3:-14517789 | MS.gene23490:CDS |
| TCTTTCGGCTACCATAATGT+CGG | 0.663564 | 7.3:-14518745 | MS.gene23490:CDS |
| GTAAGACGGAGAATCAAACC+TGG | 0.669834 | 7.3:+14518608 | None:intergenic |
| CTGCAATGACTTACTACTCG+CGG | 0.671835 | 7.3:-14518506 | MS.gene23490:CDS |
| GGCAAGAGCAAACATGAAGG+CGG | 0.671889 | 7.3:+14518041 | None:intergenic |
| ACAGATAACCATCATCATGA+GGG | 0.673555 | 7.3:+14518872 | None:intergenic |
| TCTTCATACCAATACCTGCA+GGG | 0.687460 | 7.3:+14517764 | None:intergenic |
| TGTGTTCTTAGCAACTAAAG+CGG | 0.693409 | 7.3:+14518455 | None:intergenic |
| TTGATGTGGCTAAAACACAA+TGG | 0.704796 | 7.3:-14519148 | MS.gene23490:CDS |
| GCTTTGCTGCACCATCAACG+TGG | 0.731004 | 7.3:+14519021 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AAGAATTCATTGATTTTGTT+GGG | - | chr7.3:14519028-14519047 | MS.gene23490:CDS | 20.0% |
| ! | ATTCTAATAAAAAGACTCGT+GGG | - | chr7.3:14518054-14518073 | MS.gene23490:CDS | 25.0% |
| ! | TATTCTAATAAAAAGACTCG+TGG | - | chr7.3:14518053-14518072 | MS.gene23490:CDS | 25.0% |
| !!! | GAAGAATTCATTGATTTTGT+TGG | - | chr7.3:14519027-14519046 | MS.gene23490:CDS | 25.0% |
| AAGATTTACCATCCAATTGA+TGG | - | chr7.3:14518694-14518713 | MS.gene23490:CDS | 30.0% | |
| AAGGAAAAGGCTAAATCATA+TGG | - | chr7.3:14518410-14518429 | MS.gene23490:CDS | 30.0% | |
| ACAACAAATGTTGTAGCATT+AGG | + | chr7.3:14518845-14518864 | None:intergenic | 30.0% | |
| AGATTTACCATCCAATTGAT+GGG | - | chr7.3:14518695-14518714 | MS.gene23490:CDS | 30.0% | |
| ATGATGGTTATCTGTTCAAT+TGG | - | chr7.3:14517909-14517928 | MS.gene23490:CDS | 30.0% | |
| ATTAGAATACTCCGACATTA+TGG | + | chr7.3:14518042-14518061 | None:intergenic | 30.0% | |
| CATAATTTCATCTGCATTCA+AGG | - | chr7.3:14518142-14518161 | MS.gene23490:CDS | 30.0% | |
| TAAAGAAGAATCCCATCAAT+TGG | + | chr7.3:14518709-14518728 | None:intergenic | 30.0% | |
| TTCTAATAAAAAGACTCGTG+GGG | - | chr7.3:14518055-14518074 | MS.gene23490:CDS | 30.0% | |
| ! | AAATCTTTAGAGGAAATGTC+AGG | - | chr7.3:14519106-14519125 | MS.gene23490:CDS | 30.0% |
| ! | AAGGATATTTTCAGTGCAAT+TGG | - | chr7.3:14518539-14518558 | MS.gene23490:CDS | 30.0% |
| ! | ACCTTCTTCTTTGCAAATTT+TGG | - | chr7.3:14518818-14518837 | MS.gene23490:CDS | 30.0% |
| ! | ATATTTTCAGTGCAATTGGT+TGG | - | chr7.3:14518543-14518562 | MS.gene23490:CDS | 30.0% |
| ! | CCTTCTTCTTTGCAAATTTT+GGG | - | chr7.3:14518819-14518838 | MS.gene23490:CDS | 30.0% |
| ! | TAAAGATTTTCCATTTGCCT+CGG | + | chr7.3:14519095-14519114 | None:intergenic | 30.0% |
| ! | TTGGGATGTTGTAACATTTT+GGG | - | chr7.3:14519046-14519065 | MS.gene23490:CDS | 30.0% |
| AACAGATAACCATCATCATG+AGG | + | chr7.3:14517905-14517924 | None:intergenic | 35.0% | |
| ACAGATAACCATCATCATGA+GGG | + | chr7.3:14517904-14517923 | None:intergenic | 35.0% | |
| ACATCACCACAAATCCTATT+CGG | + | chr7.3:14518790-14518809 | None:intergenic | 35.0% | |
| ACTCATAAGGATAACCGAAT+AGG | - | chr7.3:14518773-14518792 | MS.gene23490:CDS | 35.0% | |
| ATTTGCAAAGAAGAAGGTCA+AGG | + | chr7.3:14518816-14518835 | None:intergenic | 35.0% | |
| CATTTGGAGGATAATTGTGA+TGG | - | chr7.3:14518229-14518248 | MS.gene23490:CDS | 35.0% | |
| CCCAAAATTTGCAAAGAAGA+AGG | + | chr7.3:14518822-14518841 | None:intergenic | 35.0% | |
| CTATGATGATTGCAGTGAAA+TGG | + | chr7.3:14517653-14517672 | None:intergenic | 35.0% | |
| CTTGTATTTGGCACAAAACA+AGG | - | chr7.3:14518961-14518980 | MS.gene23490:CDS | 35.0% | |
| GGCAAATGGAAAATCTTTAG+AGG | - | chr7.3:14519096-14519115 | MS.gene23490:CDS | 35.0% | |
| TATTCTGTCTATGAAAGCCA+CGG | + | chr7.3:14518672-14518691 | None:intergenic | 35.0% | |
| TGCATTCGGATTCTTGTATT+TGG | - | chr7.3:14518949-14518968 | MS.gene23490:CDS | 35.0% | |
| TGTGTTCTTAGCAACTAAAG+CGG | + | chr7.3:14518321-14518340 | None:intergenic | 35.0% | |
| TTATGAACACTGGACTCATA+AGG | - | chr7.3:14518760-14518779 | MS.gene23490:CDS | 35.0% | |
| TTATGAGTCCAGTGTTCATA+AGG | + | chr7.3:14518761-14518780 | None:intergenic | 35.0% | |
| TTGATGTGGCTAAAACACAA+TGG | - | chr7.3:14517625-14517644 | MS.gene23490:CDS | 35.0% | |
| TTGCACTGAAAATATCCTTC+TGG | + | chr7.3:14518538-14518557 | None:intergenic | 35.0% | |
| ! | AAAGATTTTCCATTTGCCTC+GGG | + | chr7.3:14519094-14519113 | None:intergenic | 35.0% |
| ! | CTTTCAGGACAACTTTTCTT+TGG | - | chr7.3:14517834-14517853 | MS.gene23490:CDS | 35.0% |
| ! | GTTATCTGTTCAATTGGTTC+TGG | - | chr7.3:14517915-14517934 | MS.gene23490:CDS | 35.0% |
| ! | GTTGGGATGTTGTAACATTT+TGG | - | chr7.3:14519045-14519064 | MS.gene23490:CDS | 35.0% |
| ! | TGGGATGTTGTAACATTTTG+GGG | - | chr7.3:14519047-14519066 | MS.gene23490:CDS | 35.0% |
| !! | AGATATGTCTAAGGTTTTGC+AGG | - | chr7.3:14518355-14518374 | MS.gene23490:CDS | 35.0% |
| !! | ATTGGTTCTGGTCTTTCTTT+CGG | - | chr7.3:14517927-14517946 | MS.gene23490:CDS | 35.0% |
| !! | TATCCTTCTGGAAGAGATTT+TGG | + | chr7.3:14518526-14518545 | None:intergenic | 35.0% |
| AAAGAGTTCATGAGTCGTCA+TGG | - | chr7.3:14518443-14518462 | MS.gene23490:CDS | 40.0% | |
| AATGGCAAGAGCAAACATGA+AGG | + | chr7.3:14518738-14518757 | None:intergenic | 40.0% | |
| AATGTATCAGCTGCTGTGAA+TGG | - | chr7.3:14517792-14517811 | MS.gene23490:CDS | 40.0% | |
| AATGTCAGGTGAGAATGATG+AGG | - | chr7.3:14519120-14519139 | MS.gene23490:CDS | 40.0% | |
| ACAAACCATGAATGCACTTG+AGG | - | chr7.3:14518577-14518596 | MS.gene23490:CDS | 40.0% | |
| ACAAGAGTAAAGCAGATGCA+GGG | - | chr7.3:14518984-14519003 | MS.gene23490:CDS | 40.0% | |
| ACAGAAAGAGGAGAAGGAAA+AGG | - | chr7.3:14518397-14518416 | MS.gene23490:CDS | 40.0% | |
| ACATTATGGTAGCCGAAAGA+GGG | + | chr7.3:14518028-14518047 | None:intergenic | 40.0% | |
| AGATTTCGATCAACTTGCCA+TGG | - | chr7.3:14518887-14518906 | MS.gene23490:CDS | 40.0% | |
| AGTAGTAAGTCATTGCAGCT+GGG | + | chr7.3:14518265-14518284 | None:intergenic | 40.0% | |
| AGTCCAGTGTTCATAAGGAA+TGG | + | chr7.3:14518756-14518775 | None:intergenic | 40.0% | |
| ATGGGCAGGAAAAAAGTCTA+TGG | - | chr7.3:14517873-14517892 | MS.gene23490:CDS | 40.0% | |
| ATTCACAGCAGCTGATACAT+TGG | + | chr7.3:14517793-14517812 | None:intergenic | 40.0% | |
| GAAGAATCCCATCAATTGGA+TGG | + | chr7.3:14518705-14518724 | None:intergenic | 40.0% | |
| GCAATCATCATAGCTGGTAT+GGG | - | chr7.3:14517660-14517679 | MS.gene23490:CDS | 40.0% | |
| GGATAACCGAATAGGATTTG+TGG | - | chr7.3:14518781-14518800 | MS.gene23490:CDS | 40.0% | |
| GTGGCTTTCATAGACAGAAT+AGG | - | chr7.3:14518671-14518690 | MS.gene23490:CDS | 40.0% | |
| TAGCACCTCAATTTGATCCT+TGG | + | chr7.3:14517598-14517617 | None:intergenic | 40.0% | |
| TAGCCAAAATCTCTTCCAGA+AGG | - | chr7.3:14518520-14518539 | MS.gene23490:CDS | 40.0% | |
| TCTTCATACCAATACCTGCA+GGG | + | chr7.3:14519012-14519031 | None:intergenic | 40.0% | |
| TCTTTCGGCTACCATAATGT+CGG | - | chr7.3:14518028-14518047 | MS.gene23490:CDS | 40.0% | |
| TGCAATCATCATAGCTGGTA+TGG | - | chr7.3:14517659-14517678 | MS.gene23490:CDS | 40.0% | |
| TGGAGGATAATTGTGATGGT+AGG | - | chr7.3:14518233-14518252 | MS.gene23490:CDS | 40.0% | |
| TTCACAGCAGCTGATACATT+GGG | + | chr7.3:14517792-14517811 | None:intergenic | 40.0% | |
| TTCACTGCAATCATCATAGC+TGG | - | chr7.3:14517654-14517673 | MS.gene23490:CDS | 40.0% | |
| TTCTTCATACCAATACCTGC+AGG | + | chr7.3:14519013-14519032 | None:intergenic | 40.0% | |
| TTGCCATTCCTTATGAACAC+TGG | - | chr7.3:14518750-14518769 | MS.gene23490:CDS | 40.0% | |
| ! | AGGGTTGTCATGACAGATTT+TGG | + | chr7.3:14517960-14517979 | None:intergenic | 40.0% |
| ! | ATGCAGGGTTTCGGAATTTT+GGG | - | chr7.3:14518101-14518120 | MS.gene23490:CDS | 40.0% |
| ! | TTTTCCTTCTCCTCTTTCTG+TGG | + | chr7.3:14518398-14518417 | None:intergenic | 40.0% |
| !! | CAAGTTGATCGAAATCTAGC+TGG | + | chr7.3:14518884-14518903 | None:intergenic | 40.0% |
| AACCATGAATGCACTTGAGG+AGG | - | chr7.3:14518580-14518599 | MS.gene23490:CDS | 45.0% | |
| AACCTCCTCAAGTGCATTCA+TGG | + | chr7.3:14518585-14518604 | None:intergenic | 45.0% | |
| ACTGGATCAACCTCGTAAGA+CGG | + | chr7.3:14518182-14518201 | None:intergenic | 45.0% | |
| AGCTGCAGCAGATATGTCTA+AGG | - | chr7.3:14518346-14518365 | MS.gene23490:CDS | 45.0% | |
| CAAAATTCCGAAACCCTGCA+TGG | + | chr7.3:14518102-14518121 | None:intergenic | 45.0% | |
| CATGGAATATCATCCGCATC+GGG | - | chr7.3:14518905-14518924 | MS.gene23490:CDS | 45.0% | |
| CCAAAACCGGAAGAAGCAAA+GGG | + | chr7.3:14517979-14517998 | None:intergenic | 45.0% | |
| CCATGGAATATCATCCGCAT+CGG | - | chr7.3:14518904-14518923 | MS.gene23490:CDS | 45.0% | |
| CCTCCAAATGTAGTCTGCTT+GGG | + | chr7.3:14518219-14518238 | None:intergenic | 45.0% | |
| CGCATATACTACCACGTTGA+TGG | - | chr7.3:14517741-14517760 | MS.gene23490:CDS | 45.0% | |
| CTAGCTGGAAAAATCTCTGC+TGG | + | chr7.3:14518869-14518888 | None:intergenic | 45.0% | |
| CTCCAAATGTAGTCTGCTTG+GGG | + | chr7.3:14518218-14518237 | None:intergenic | 45.0% | |
| CTGCAATGACTTACTACTCG+CGG | - | chr7.3:14518267-14518286 | MS.gene23490:CDS | 45.0% | |
| CTTCTCCTCTTTCTGTGGTT+CGG | + | chr7.3:14518393-14518412 | None:intergenic | 45.0% | |
| GACAAGAGTAAAGCAGATGC+AGG | - | chr7.3:14518983-14519002 | MS.gene23490:CDS | 45.0% | |
| GACATTATGGTAGCCGAAAG+AGG | + | chr7.3:14518029-14518048 | None:intergenic | 45.0% | |
| GAGTAGTAAGTCATTGCAGC+TGG | + | chr7.3:14518266-14518285 | None:intergenic | 45.0% | |
| GCTTTCTGTGGAACACTTTC+AGG | - | chr7.3:14517819-14517838 | MS.gene23490:CDS | 45.0% | |
| GGTGAGAATGATGAGGAAGT+TGG | - | chr7.3:14519127-14519146 | MS.gene23490:CDS | 45.0% | |
| GTAAGACGGAGAATCAAACC+TGG | + | chr7.3:14518168-14518187 | None:intergenic | 45.0% | |
| GTTTGATTCTCCGTCTTACG+AGG | - | chr7.3:14518169-14518188 | MS.gene23490:CDS | 45.0% | |
| TCCCTTGTTACCAAGTTGCT+CGG | - | chr7.3:14517717-14517736 | MS.gene23490:CDS | 45.0% | |
| TCCTCCAAATGTAGTCTGCT+TGG | + | chr7.3:14518220-14518239 | None:intergenic | 45.0% | |
| TGACAACCCTTTGCTTCTTC+CGG | - | chr7.3:14517970-14517989 | MS.gene23490:CDS | 45.0% | |
| TGCTTGGTACAACAAGCACT+TGG | - | chr7.3:14518474-14518493 | MS.gene23490:CDS | 45.0% | |
| TGGTATGACCCTCATGATGA+TGG | - | chr7.3:14517893-14517912 | MS.gene23490:CDS | 45.0% | |
| TTCCCCAAGCAGACTACATT+TGG | - | chr7.3:14518213-14518232 | MS.gene23490:CDS | 45.0% | |
| TTCTTAGTACCCGAGGCAAA+TGG | - | chr7.3:14519082-14519101 | MS.gene23490:CDS | 45.0% | |
| TTTCATCTGCATTCAAGGCC+AGG | - | chr7.3:14518147-14518166 | MS.gene23490:CDS | 45.0% | |
| ! | ACCGAGCAACTTGGTAACAA+GGG | + | chr7.3:14517721-14517740 | None:intergenic | 45.0% |
| ! | AGTGCATTCATGGTTTGTGC+AGG | + | chr7.3:14518575-14518594 | None:intergenic | 45.0% |
| ! | CAGGACAACTTTTCTTTGGC+TGG | - | chr7.3:14517838-14517857 | MS.gene23490:CDS | 45.0% |
| ! | CATGCAGGGTTTCGGAATTT+TGG | - | chr7.3:14518100-14518119 | MS.gene23490:CDS | 45.0% |
| ! | CATTCATGGTTTGTGCAGGA+GGG | + | chr7.3:14518571-14518590 | None:intergenic | 45.0% |
| ! | CGTCATGGACTACATTTGCT+TGG | - | chr7.3:14518458-14518477 | MS.gene23490:CDS | 45.0% |
| ! | GCGTTGTTATTCTGAGCATG+AGG | + | chr7.3:14519175-14519194 | None:intergenic | 45.0% |
| ! | TCTTACGAGGTTGATCCAGT+TGG | - | chr7.3:14518182-14518201 | MS.gene23490:CDS | 45.0% |
| !! | CAACTTTTCTTTGGCTGGCT+TGG | - | chr7.3:14517843-14517862 | MS.gene23490:CDS | 45.0% |
| !! | GTGAATGGTGTTGCTTTCTG+TGG | - | chr7.3:14517807-14517826 | MS.gene23490:CDS | 45.0% |
| !!! | TTTTGGCTTGGGTTCGGTAT+TGG | - | chr7.3:14517993-14518012 | MS.gene23490:CDS | 45.0% |
| ACAGCAGCTGATACATTGGG+AGG | + | chr7.3:14517789-14517808 | None:intergenic | 50.0% | |
| CCCAAGCAGACTACATTTGG+AGG | - | chr7.3:14518216-14518235 | MS.gene23490:CDS | 50.0% | |
| CCGATGCGGATGATATTCCA+TGG | + | chr7.3:14518907-14518926 | None:intergenic | 50.0% | |
| CGAACCACAGAAAGAGGAGA+AGG | - | chr7.3:14518391-14518410 | MS.gene23490:CDS | 50.0% | |
| GCAGTACAGTACCAGGCTAT+TGG | - | chr7.3:14518642-14518661 | MS.gene23490:CDS | 50.0% | |
| GCCAAAACCGGAAGAAGCAA+AGG | + | chr7.3:14517980-14517999 | None:intergenic | 50.0% | |
| GCGGTATAACGAGCAGTTTC+TGG | + | chr7.3:14518302-14518321 | None:intergenic | 50.0% | |
| GCTCTATGCAGTACAGTACC+AGG | - | chr7.3:14518635-14518654 | MS.gene23490:CDS | 50.0% | |
| GGAAAGCTTGGGGCTATAGT+TGG | - | chr7.3:14518926-14518945 | MS.gene23490:CDS | 50.0% | |
| GGCAAAGACTGCTGCAATGA+AGG | + | chr7.3:14518081-14518100 | None:intergenic | 50.0% | |
| GGCAAGAGCAAACATGAAGG+CGG | + | chr7.3:14518735-14518754 | None:intergenic | 50.0% | |
| GGTGCTAAATGCGCTTGATG+TGG | - | chr7.3:14517611-14517630 | MS.gene23490:CDS | 50.0% | |
| GTATATGCGACCGAGCAACT+TGG | + | chr7.3:14517730-14517749 | None:intergenic | 50.0% | |
| GTCTTTGCCATGCAGGGTTT+CGG | - | chr7.3:14518092-14518111 | MS.gene23490:CDS | 50.0% | |
| GTGCACTTTCTTAGTACCCG+AGG | - | chr7.3:14519075-14519094 | MS.gene23490:CDS | 50.0% | |
| TACATTGGGAGGCAACGTAC+CGG | + | chr7.3:14517778-14517797 | None:intergenic | 50.0% | |
| TACCGAACCCAAGCCAAAAC+CGG | + | chr7.3:14517992-14518011 | None:intergenic | 50.0% | |
| TGAAGCCGAACCACAGAAAG+AGG | - | chr7.3:14518385-14518404 | MS.gene23490:CDS | 50.0% | |
| TGGGGAACAGTTGAACCAAC+TGG | + | chr7.3:14518200-14518219 | None:intergenic | 50.0% | |
| ! | CCCTTTGCTTCTTCCGGTTT+TGG | - | chr7.3:14517976-14517995 | MS.gene23490:CDS | 50.0% |
| ! | GACCGAGCAACTTGGTAACA+AGG | + | chr7.3:14517722-14517741 | None:intergenic | 50.0% |
| ! | GCATTCATGGTTTGTGCAGG+AGG | + | chr7.3:14518572-14518591 | None:intergenic | 50.0% |
| ! | GGGGCTATAGTTGGTGCATT+CGG | - | chr7.3:14518935-14518954 | MS.gene23490:CDS | 50.0% |
| ! | TGGTGGTGACTACCCTCTTT+CGG | - | chr7.3:14518013-14518032 | MS.gene23490:CDS | 50.0% |
| !! | ACCAGGCTATTGGTTTACCG+TGG | - | chr7.3:14518652-14518671 | MS.gene23490:CDS | 50.0% |
| !! | GGCTGGCTTGGTGATAAGAT+GGG | - | chr7.3:14517855-14517874 | MS.gene23490:CDS | 50.0% |
| !! | GGTTTCGGAATTTTGGGAGC+TGG | - | chr7.3:14518107-14518126 | MS.gene23490:CDS | 50.0% |
| !! | TGGCTGGCTTGGTGATAAGA+TGG | - | chr7.3:14517854-14517873 | MS.gene23490:CDS | 50.0% |
| !!! | GCTTCTTCCGGTTTTGGCTT+GGG | - | chr7.3:14517982-14518001 | MS.gene23490:CDS | 50.0% |
| !!! | TGCTTCTTCCGGTTTTGGCT+TGG | - | chr7.3:14517981-14518000 | MS.gene23490:CDS | 50.0% |
| !!! | TTCCGGTTTTGGCTTGGGTT+CGG | - | chr7.3:14517987-14518006 | MS.gene23490:CDS | 50.0% |
| ACATTGGGAGGCAACGTACC+GGG | + | chr7.3:14517777-14517796 | None:intergenic | 55.0% | |
| ATCCGCATCGGGAAAGCTTG+GGG | - | chr7.3:14518916-14518935 | MS.gene23490:CDS | 55.0% | |
| CATCCGCATCGGGAAAGCTT+GGG | - | chr7.3:14518915-14518934 | MS.gene23490:CDS | 55.0% | |
| GCAGCAGTCTTTGCCATGCA+GGG | - | chr7.3:14518086-14518105 | MS.gene23490:CDS | 55.0% | |
| GCCACGGTAAACCAATAGCC+TGG | + | chr7.3:14518656-14518675 | None:intergenic | 55.0% | |
| GCTTTGCTGCACCATCAACG+TGG | + | chr7.3:14517755-14517774 | None:intergenic | 55.0% | |
| TCATCCGCATCGGGAAAGCT+TGG | - | chr7.3:14518914-14518933 | MS.gene23490:CDS | 55.0% | |
| TGCAGCAGTCTTTGCCATGC+AGG | - | chr7.3:14518085-14518104 | MS.gene23490:CDS | 55.0% | |
| ! | TGGCTTGGGTTCGGTATTGG+TGG | - | chr7.3:14517996-14518015 | MS.gene23490:CDS | 55.0% |
| !! | GGCTTGGTGATAAGATGGGC+AGG | - | chr7.3:14517859-14517878 | MS.gene23490:CDS | 55.0% |
| !! | GTTGATGGTGCAGCAAAGCC+CGG | - | chr7.3:14517756-14517775 | MS.gene23490:CDS | 55.0% |
| AGCCCCAAGCTTTCCCGATG+CGG | + | chr7.3:14518921-14518940 | None:intergenic | 60.0% | |
| ! | GCAGGGTACCCTGCAGGTAT+TGG | - | chr7.3:14519001-14519020 | MS.gene23490:CDS | 60.0% |
| ! | GCAGATGCAGGGTACCCTGC+AGG | - | chr7.3:14518995-14519014 | MS.gene23490:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.3 | gene | 14517591 | 14519204 | 14517591 | ID=MS.gene23490 |
| chr7.3 | mRNA | 14517591 | 14519204 | 14517591 | ID=MS.gene23490.t1;Parent=MS.gene23490 |
| chr7.3 | exon | 14517591 | 14519204 | 14517591 | ID=MS.gene23490.t1.exon1;Parent=MS.gene23490.t1 |
| chr7.3 | CDS | 14517591 | 14519204 | 14517591 | ID=cds.MS.gene23490.t1;Parent=MS.gene23490.t1 |
| Gene Sequence |
| Protein sequence |
