Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene060856.t1 | XP_003592049.1 | 100 | 117 | 0 | 0 | 1 | 117 | 1 | 117 | 2.40E-58 | 234.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene060856.t1 | P34788 | 90.6 | 117 | 11 | 0 | 1 | 117 | 1 | 117 | 5.6e-56 | 218.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene060856.t1 | A0A072W005 | 100.0 | 117 | 0 | 0 | 1 | 117 | 1 | 117 | 1.7e-58 | 234.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050290 | MS.gene060856 | 0.879189 | 1.53E-69 | -1.69E-46 |
| MS.gene054937 | MS.gene060856 | 0.869501 | 2.95E-66 | -1.69E-46 |
| MS.gene057092 | MS.gene060856 | 0.808743 | 2.67E-50 | -1.69E-46 |
| MS.gene057235 | MS.gene060856 | 0.803991 | 2.68E-49 | -1.69E-46 |
| MS.gene060853 | MS.gene060856 | 0.836467 | 9.20E-57 | -1.69E-46 |
| MS.gene060856 | MS.gene06835 | 0.816244 | 6.11E-52 | -1.69E-46 |
| MS.gene060856 | MS.gene069310 | 0.808843 | 2.54E-50 | -1.69E-46 |
| MS.gene060856 | MS.gene069962 | 0.835124 | 2.01E-56 | -1.69E-46 |
| MS.gene060856 | MS.gene07018 | 0.843004 | 1.83E-58 | -1.69E-46 |
| MS.gene060856 | MS.gene073078 | 0.825242 | 5.22E-54 | -1.69E-46 |
| MS.gene060856 | MS.gene074262 | 0.8636 | 2.22E-64 | -1.69E-46 |
| MS.gene060856 | MS.gene074594 | 0.80978 | 1.60E-50 | -1.69E-46 |
| MS.gene060856 | MS.gene21636 | 0.851724 | 7.36E-61 | -1.69E-46 |
| MS.gene060856 | MS.gene22507 | 0.830666 | 2.58E-55 | -1.69E-46 |
| MS.gene060856 | MS.gene23123 | 0.833097 | 6.49E-56 | -1.69E-46 |
| MS.gene060856 | MS.gene23437 | 0.854597 | 1.11E-61 | -1.69E-46 |
| MS.gene060856 | MS.gene23883 | 0.812932 | 3.31E-51 | -1.69E-46 |
| MS.gene060856 | MS.gene24054 | 0.819433 | 1.16E-52 | -1.69E-46 |
| MS.gene060856 | MS.gene24706 | 0.87967 | 1.03E-69 | -1.69E-46 |
| MS.gene060856 | MS.gene34149 | 0.845737 | 3.37E-59 | -1.69E-46 |
| MS.gene060856 | MS.gene34283 | 0.836326 | 9.99E-57 | -1.69E-46 |
| MS.gene060856 | MS.gene34284 | 0.839356 | 1.66E-57 | -1.69E-46 |
| MS.gene060856 | MS.gene34285 | 0.834124 | 3.59E-56 | -1.69E-46 |
| MS.gene060856 | MS.gene36490 | 0.822745 | 2.01E-53 | -1.69E-46 |
| MS.gene060856 | MS.gene38222 | 0.812382 | 4.36E-51 | -1.69E-46 |
| MS.gene060856 | MS.gene40831 | 0.817375 | 3.41E-52 | -1.69E-46 |
| MS.gene060856 | MS.gene43070 | 0.823034 | 1.72E-53 | -1.69E-46 |
| MS.gene060856 | MS.gene43071 | 0.81822 | 2.20E-52 | -1.69E-46 |
| MS.gene060856 | MS.gene43173 | 0.813681 | 2.26E-51 | -1.69E-46 |
| MS.gene060856 | MS.gene48048 | 0.847706 | 9.76E-60 | -1.69E-46 |
| MS.gene060856 | MS.gene50252 | 0.821862 | 3.22E-53 | -1.69E-46 |
| MS.gene060856 | MS.gene57391 | 0.859471 | 4.04E-63 | -1.69E-46 |
| MS.gene060856 | MS.gene58148 | 0.836494 | 9.06E-57 | -1.69E-46 |
| MS.gene060856 | MS.gene61251 | 0.828723 | 7.67E-55 | -1.69E-46 |
| MS.gene060856 | MS.gene62652 | 0.870181 | 1.77E-66 | -1.69E-46 |
| MS.gene060856 | MS.gene65076 | 0.803175 | 3.95E-49 | -1.69E-46 |
| MS.gene060856 | MS.gene67681 | 0.810205 | 1.29E-50 | -1.69E-46 |
| MS.gene060856 | MS.gene70215 | 0.85654 | 3.00E-62 | -1.69E-46 |
| MS.gene060856 | MS.gene70292 | 0.837869 | 4.03E-57 | -1.69E-46 |
| MS.gene060856 | MS.gene70805 | 0.835699 | 1.44E-56 | -1.69E-46 |
| MS.gene060856 | MS.gene87309 | 0.856802 | 2.51E-62 | -1.69E-46 |
| MS.gene060856 | MS.gene88892 | 0.902131 | 1.33E-78 | -1.69E-46 |
| MS.gene060856 | MS.gene91826 | 0.827981 | 1.16E-54 | -1.69E-46 |
| MS.gene060856 | MS.gene94561 | 0.816573 | 5.16E-52 | -1.69E-46 |
| MS.gene060856 | MS.gene95828 | 0.838146 | 3.42E-57 | -1.69E-46 |
| MS.gene060856 | MS.gene95856 | 0.825075 | 5.71E-54 | -1.69E-46 |
| MS.gene060856 | MS.gene98113 | 0.825322 | 4.99E-54 | -1.69E-46 |
| MS.gene060856 | MS.gene99709 | 0.808943 | 2.42E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene060856.t1 | MTR_1g098170 | 100.000 | 117 | 0 | 0 | 1 | 117 | 1 | 117 | 7.66e-84 | 241 |
| MS.gene060856.t1 | MTR_1g098220 | 100.000 | 117 | 0 | 0 | 1 | 117 | 1 | 117 | 7.66e-84 | 241 |
| MS.gene060856.t1 | MTR_1g098220 | 100.000 | 117 | 0 | 0 | 1 | 117 | 1 | 117 | 7.75e-84 | 241 |
| MS.gene060856.t1 | MTR_6g043210 | 98.291 | 117 | 2 | 0 | 1 | 117 | 1 | 117 | 6.03e-83 | 239 |
| MS.gene060856.t1 | MTR_1g492710 | 97.436 | 117 | 3 | 0 | 1 | 117 | 794 | 910 | 3.44e-73 | 235 |
| MS.gene060856.t1 | MTR_5g027650 | 94.937 | 79 | 4 | 0 | 39 | 117 | 11 | 89 | 6.13e-50 | 154 |
| MS.gene060856.t1 | MTR_5g027580 | 93.671 | 79 | 5 | 0 | 39 | 117 | 48 | 126 | 2.04e-49 | 154 |
| MS.gene060856.t1 | MTR_5g027670 | 95.455 | 66 | 3 | 0 | 52 | 117 | 1 | 66 | 4.20e-41 | 131 |
| MS.gene060856.t1 | MTR_5g027615 | 95.455 | 66 | 3 | 0 | 52 | 117 | 1 | 66 | 1.82e-40 | 129 |
| MS.gene060856.t1 | MTR_5g027605 | 95.455 | 66 | 3 | 0 | 52 | 117 | 1 | 66 | 1.82e-40 | 129 |
| MS.gene060856.t1 | MTR_5g027640 | 95.455 | 66 | 3 | 0 | 52 | 117 | 1 | 66 | 1.82e-40 | 129 |
| MS.gene060856.t1 | MTR_5g027600 | 95.455 | 66 | 3 | 0 | 52 | 117 | 1 | 66 | 1.82e-40 | 129 |
| MS.gene060856.t1 | MTR_1005s0010 | 95.238 | 63 | 3 | 0 | 55 | 117 | 2 | 64 | 5.14e-39 | 126 |
| MS.gene060856.t1 | MTR_4g015960 | 91.837 | 49 | 4 | 0 | 68 | 116 | 10 | 58 | 8.61e-26 | 100 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene060856.t1 | AT4G09800 | 90.598 | 117 | 11 | 0 | 1 | 117 | 1 | 117 | 2.61e-77 | 225 |
| MS.gene060856.t1 | AT1G34030 | 90.598 | 117 | 11 | 0 | 1 | 117 | 1 | 117 | 2.61e-77 | 225 |
| MS.gene060856.t1 | AT1G22780 | 90.598 | 117 | 11 | 0 | 1 | 117 | 1 | 117 | 2.61e-77 | 225 |
Find 23 sgRNAs with CRISPR-Local
Find 40 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAGCTTCATGTCAAGTTGAT+TGG | 0.350481 | 1.1:-70600252 | None:intergenic |
| CATGAAGCTGAGGGATGATT+TGG | 0.353151 | 1.1:+70600265 | MS.gene060856:CDS |
| TGATTGAGGTCATTGCGAAC+TGG | 0.463060 | 1.1:-70599877 | None:intergenic |
| GTTCGCAATGACCTCAATCA+AGG | 0.484324 | 1.1:+70599880 | MS.gene060856:CDS |
| ATGACCTCAATCAAGGGTAT+TGG | 0.493197 | 1.1:+70599887 | MS.gene060856:CDS |
| GTCAATTCAAGGTTCCAGAT+TGG | 0.495981 | 1.1:+70600177 | MS.gene060856:CDS |
| CGCTAACATCTGCTGTAAGA+AGG | 0.503592 | 1.1:+70599919 | MS.gene060856:CDS |
| TGCGAATCCTCGTCAATTCA+AGG | 0.503849 | 1.1:+70600166 | MS.gene060856:CDS |
| TGGAGAGACTCAAGAAGATC+CGG | 0.515884 | 1.1:+70600285 | MS.gene060856:CDS |
| GAACAGAAAGAAGGATTACA+AGG | 0.525479 | 1.1:+70600205 | MS.gene060856:CDS |
| AGAAAGAAGGATTACAAGGA+TGG | 0.565673 | 1.1:+70600209 | MS.gene060856:CDS |
| TGAATTGAGTGCCGCAGAGT+TGG | 0.583506 | 1.1:+70600124 | MS.gene060856:CDS |
| CCTACCAATACCCTTGATTG+AGG | 0.590405 | 1.1:-70599891 | None:intergenic |
| ATCAACTTGACATGAAGCTG+AGG | 0.604543 | 1.1:+70600255 | MS.gene060856:CDS |
| GTTGGATAATATTATGACAG+TGG | 0.620536 | 1.1:+70600142 | MS.gene060856:CDS |
| TTCGCAATGACCTCAATCAA+GGG | 0.622158 | 1.1:+70599881 | MS.gene060856:CDS |
| CCTCAATCAAGGGTATTGGT+AGG | 0.631523 | 1.1:+70599891 | MS.gene060856:CDS |
| GTGTTGAACACAAATGTCGA+TGG | 0.634706 | 1.1:+70599842 | MS.gene060856:CDS |
| TCAACTTGACATGAAGCTGA+GGG | 0.637423 | 1.1:+70600256 | MS.gene060856:CDS |
| CCTCTTGTTCATGTCAACAT+CGG | 0.651019 | 1.1:-70599942 | None:intergenic |
| CCGATGTTGACATGAACAAG+AGG | 0.658386 | 1.1:+70599942 | MS.gene060856:CDS |
| TCTGGAACCTTGAATTGACG+AGG | 0.669518 | 1.1:-70600173 | None:intergenic |
| CATAATATTATCCAACTCTG+CGG | 0.698001 | 1.1:-70600135 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATTTTTGTTGTTAAAATTAA+TGG | + | chr1.1:70600005-70600024 | MS.gene060856:intron | 10.0% |
| !!! | TTTTTGTTGTTAAAATTAAT+GGG | + | chr1.1:70600006-70600025 | MS.gene060856:intron | 10.0% |
| !! | TTTGATATTATTGTTGAAAT+GGG | + | chr1.1:70600069-70600088 | MS.gene060856:intron | 15.0% |
| !!! | TTTTGATATTATTGTTGAAA+TGG | + | chr1.1:70600068-70600087 | MS.gene060856:intron | 15.0% |
| !!! | AAATGACTGATTTATAAAAG+TGG | - | chr1.1:70599989-70600008 | None:intergenic | 20.0% |
| !!! | AATGACTGATTTATAAAAGT+GGG | - | chr1.1:70599988-70600007 | None:intergenic | 20.0% |
| !!! | TTTGTTGTTGGATTTTTTTA+GGG | + | chr1.1:70600097-70600116 | MS.gene060856:intron | 20.0% |
| !!! | TTTTGTTGTTGGATTTTTTT+AGG | + | chr1.1:70600096-70600115 | MS.gene060856:intron | 20.0% |
| AGTGAAGAAAGAGATGAAAT+CGG | - | chr1.1:70599734-70599753 | None:intergenic | 30.0% | |
| CATAATATTATCCAACTCTG+CGG | - | chr1.1:70600138-70600157 | None:intergenic | 30.0% | |
| GTTGGATAATATTATGACAG+TGG | + | chr1.1:70600142-70600161 | MS.gene060856:CDS | 30.0% | |
| TTTCTGTTCAAAAACCAATC+TGG | - | chr1.1:70600194-70600213 | None:intergenic | 30.0% | |
| !! | TTGGTTTTTGAACAGAAAGA+AGG | + | chr1.1:70600196-70600215 | MS.gene060856:CDS | 30.0% |
| !!! | AAATGGGTTGTTTTTGTTGT+TGG | + | chr1.1:70600085-70600104 | MS.gene060856:intron | 30.0% |
| ACGAAAAGTAACAGAATCAG+TGG | - | chr1.1:70599771-70599790 | None:intergenic | 35.0% | |
| AGAAAGAAGGATTACAAGGA+TGG | + | chr1.1:70600209-70600228 | MS.gene060856:CDS | 35.0% | |
| GAACAGAAAGAAGGATTACA+AGG | + | chr1.1:70600205-70600224 | MS.gene060856:CDS | 35.0% | |
| GTGAAGAAAGAGATGAAATC+GGG | - | chr1.1:70599733-70599752 | None:intergenic | 35.0% | |
| TCGTGATGTATTACAGTCTT+TGG | + | chr1.1:70599787-70599806 | MS.gene060856:intron | 35.0% | |
| !!! | TTGTTGGATTTTTTTAGGGC+TGG | + | chr1.1:70600101-70600120 | MS.gene060856:intron | 35.0% |
| ATCAACTTGACATGAAGCTG+AGG | + | chr1.1:70600255-70600274 | MS.gene060856:CDS | 40.0% | |
| CCTCTTGTTCATGTCAACAT+CGG | - | chr1.1:70599945-70599964 | None:intergenic | 40.0% | |
| GAAAGAGATGAAATCGGGAT+CGG | - | chr1.1:70599728-70599747 | None:intergenic | 40.0% | |
| GTCAATTCAAGGTTCCAGAT+TGG | + | chr1.1:70600177-70600196 | MS.gene060856:CDS | 40.0% | |
| GTGTTGAACACAAATGTCGA+TGG | + | chr1.1:70599842-70599861 | MS.gene060856:CDS | 40.0% | |
| TCAACTTGACATGAAGCTGA+GGG | + | chr1.1:70600256-70600275 | MS.gene060856:CDS | 40.0% | |
| TTCGCAATGACCTCAATCAA+GGG | + | chr1.1:70599881-70599900 | MS.gene060856:CDS | 40.0% | |
| ! | ATGACCTCAATCAAGGGTAT+TGG | + | chr1.1:70599887-70599906 | MS.gene060856:CDS | 40.0% |
| !! | CAGCTTCATGTCAAGTTGAT+TGG | - | chr1.1:70600255-70600274 | None:intergenic | 40.0% |
| CATGAAGCTGAGGGATGATT+TGG | + | chr1.1:70600265-70600284 | MS.gene060856:CDS | 45.0% | |
| CCGATGTTGACATGAACAAG+AGG | + | chr1.1:70599942-70599961 | MS.gene060856:CDS | 45.0% | |
| CCTACCAATACCCTTGATTG+AGG | - | chr1.1:70599894-70599913 | None:intergenic | 45.0% | |
| CGCTAACATCTGCTGTAAGA+AGG | + | chr1.1:70599919-70599938 | MS.gene060856:CDS | 45.0% | |
| GTTCGCAATGACCTCAATCA+AGG | + | chr1.1:70599880-70599899 | MS.gene060856:CDS | 45.0% | |
| TGATTGAGGTCATTGCGAAC+TGG | - | chr1.1:70599880-70599899 | None:intergenic | 45.0% | |
| TGCGAATCCTCGTCAATTCA+AGG | + | chr1.1:70600166-70600185 | MS.gene060856:CDS | 45.0% | |
| ! | TCTGGAACCTTGAATTGACG+AGG | - | chr1.1:70600176-70600195 | None:intergenic | 45.0% |
| !! | CCTCAATCAAGGGTATTGGT+AGG | + | chr1.1:70599891-70599910 | MS.gene060856:CDS | 45.0% |
| ! | TGAATTGAGTGCCGCAGAGT+TGG | + | chr1.1:70600124-70600143 | MS.gene060856:CDS | 50.0% |
| CGGGATCGGAAACGTACCAT+CGG | - | chr1.1:70599714-70599733 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.1 | gene | 70599714 | 70600306 | 70599714 | ID=MS.gene060856 |
| chr1.1 | mRNA | 70599714 | 70600306 | 70599714 | ID=MS.gene060856.t1;Parent=MS.gene060856 |
| chr1.1 | exon | 70599714 | 70599716 | 70599714 | ID=MS.gene060856.t1.exon1;Parent=MS.gene060856.t1 |
| chr1.1 | CDS | 70599714 | 70599716 | 70599714 | ID=cds.MS.gene060856.t1;Parent=MS.gene060856.t1 |
| chr1.1 | exon | 70599803 | 70599963 | 70599803 | ID=MS.gene060856.t1.exon2;Parent=MS.gene060856.t1 |
| chr1.1 | CDS | 70599803 | 70599963 | 70599803 | ID=cds.MS.gene060856.t1;Parent=MS.gene060856.t1 |
| chr1.1 | exon | 70600118 | 70600306 | 70600118 | ID=MS.gene060856.t1.exon3;Parent=MS.gene060856.t1 |
| chr1.1 | CDS | 70600118 | 70600306 | 70600118 | ID=cds.MS.gene060856.t1;Parent=MS.gene060856.t1 |
| Gene Sequence |
| Protein sequence |
