AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene23957

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene23957.t1 MTR_2g103650 97.449 392 10 0 1 392 13 404 0.0 786
MS.gene23957.t1 MTR_5g043350 47.230 379 189 4 14 390 27 396 1.27e-117 348
MS.gene23957.t1 MTR_3g054320 46.950 377 184 5 16 390 30 392 1.06e-112 335
MS.gene23957.t1 MTR_3g084380 46.842 380 188 5 14 390 19 387 3.14e-112 334
MS.gene23957.t1 MTR_6g028080 44.619 381 194 6 15 390 29 397 1.47e-111 332
MS.gene23957.t1 MTR_3g084360 44.444 378 198 4 15 390 23 390 1.57e-109 327
MS.gene23957.t1 MTR_4g084050 43.864 383 196 5 16 390 23 394 1.76e-108 324
MS.gene23957.t1 MTR_8g010080 45.358 377 183 8 16 390 31 386 6.48e-105 315
MS.gene23957.t1 MTR_0157s0060 46.277 376 182 5 19 390 1 360 8.70e-104 311
MS.gene23957.t1 MTR_4g034020 46.277 376 182 5 19 390 1 360 8.70e-104 311
MS.gene23957.t1 MTR_8g010070 43.536 379 193 7 16 392 31 390 2.30e-101 306
MS.gene23957.t1 MTR_8g010040 43.799 379 192 8 2 379 18 376 3.48e-96 292
MS.gene23957.t1 MTR_1g012390 41.667 384 211 7 12 392 24 397 4.04e-96 293
MS.gene23957.t1 MTR_2g103410 42.350 366 205 5 16 380 59 419 6.00e-95 291
MS.gene23957.t1 MTR_1g052315 42.407 349 194 4 11 358 59 401 1.23e-93 288
MS.gene23957.t1 MTR_8g010110 42.440 377 195 7 16 390 31 387 1.21e-91 281
MS.gene23957.t1 MTR_8g008765 44.713 331 162 8 16 344 31 342 1.16e-90 277
MS.gene23957.t1 MTR_8g010090 41.005 378 199 9 16 390 31 387 1.39e-88 273
MS.gene23957.t1 MTR_8g065030 39.945 363 210 5 17 378 66 421 3.50e-88 274
MS.gene23957.t1 MTR_7g091850 41.160 362 205 6 1 360 61 416 6.60e-85 266
MS.gene23957.t1 MTR_6g005630 38.281 384 221 10 11 392 40 409 2.51e-84 263
MS.gene23957.t1 MTR_1g013740 37.265 373 210 6 10 381 29 378 1.82e-83 260
MS.gene23957.t1 MTR_8g010050 38.992 377 175 7 16 390 31 354 1.31e-82 256
MS.gene23957.t1 MTR_7g091840 43.077 325 178 6 9 330 57 377 4.31e-82 256
MS.gene23957.t1 MTR_4g094715 36.082 388 236 7 11 390 60 443 9.52e-82 258
MS.gene23957.t1 MTR_1g086390 36.364 385 235 7 16 392 81 463 2.53e-79 252
MS.gene23957.t1 MTR_2g060350 39.650 343 199 5 18 358 76 412 4.84e-79 250
MS.gene23957.t1 MTR_8g077320 38.501 387 169 9 16 389 33 363 8.51e-79 247
MS.gene23957.t1 MTR_1g013730 35.925 373 215 6 10 381 21 370 1.16e-78 247
MS.gene23957.t1 MTR_7g035415 42.675 314 165 5 7 318 31 331 3.41e-78 244
MS.gene23957.t1 MTR_5g034090 35.222 406 229 10 13 390 118 517 9.28e-78 249
MS.gene23957.t1 MTR_3g438050 37.297 370 216 8 13 377 24 382 4.73e-77 243
MS.gene23957.t1 MTR_7g110050 34.197 386 241 7 16 392 67 448 1.04e-76 245
MS.gene23957.t1 MTR_8g065010 35.294 408 229 10 12 390 103 504 1.12e-76 246
MS.gene23957.t1 MTR_5g033680 34.771 371 232 6 15 380 58 423 1.88e-74 239
MS.gene23957.t1 MTR_8g008700 41.325 317 146 7 78 392 14 292 1.92e-74 233
MS.gene23957.t1 MTR_3g030540 40.741 351 200 5 11 360 65 408 1.82e-73 236
MS.gene23957.t1 MTR_6g005630 35.938 384 207 12 11 392 40 386 4.49e-71 228
MS.gene23957.t1 MTR_2g032710 34.625 387 241 7 12 390 63 445 5.66e-69 224
MS.gene23957.t1 MTR_4g083660 37.726 387 224 9 8 390 1 374 3.70e-68 220
MS.gene23957.t1 MTR_4g120320 38.268 358 207 10 10 361 46 395 1.22e-67 220
MS.gene23957.t1 MTR_1g088160 36.415 357 216 7 16 369 35 383 5.93e-67 218
MS.gene23957.t1 MTR_2g049400 36.719 384 222 12 1 378 11 379 7.04e-67 217
MS.gene23957.t1 MTR_8g030930 34.115 384 232 9 16 390 27 398 5.36e-65 213
MS.gene23957.t1 MTR_2g103410 43.346 263 144 4 16 277 59 317 7.56e-65 210
MS.gene23957.t1 MTR_7g113910 37.829 304 153 5 79 381 74 342 8.41e-62 203
MS.gene23957.t1 MTR_8g009810 34.417 369 223 9 15 378 34 388 7.48e-61 202
MS.gene23957.t1 MTR_2g058670 35.843 332 204 6 34 361 41 367 1.71e-60 201
MS.gene23957.t1 MTR_2g058640 36.119 335 199 8 34 361 41 367 2.72e-60 201
MS.gene23957.t1 MTR_8g031180 41.751 297 135 8 79 368 15 280 4.01e-60 196
MS.gene23957.t1 MTR_5g063430 38.614 303 166 9 16 313 25 312 3.73e-59 195
MS.gene23957.t1 MTR_2g435640 35.965 342 208 8 16 353 53 387 7.75e-59 197
MS.gene23957.t1 MTR_2g058840 34.604 341 194 8 34 361 41 365 1.00e-58 197
MS.gene23957.t1 MTR_2g029170 36.571 350 196 12 50 390 29 361 6.15e-58 193
MS.gene23957.t1 MTR_5g034090 36.770 291 154 6 13 277 118 404 2.71e-57 193
MS.gene23957.t1 MTR_5g097860 33.724 341 207 10 1 334 10 338 1.18e-53 181
MS.gene23957.t1 MTR_2g100040 29.396 381 200 13 1 374 10 328 5.63e-39 143
MS.gene23957.t1 MTR_1g100040 25.187 401 247 12 20 385 60 442 8.46e-23 100
MS.gene23957.t1 MTR_7g088550 28.710 310 167 12 20 293 54 345 1.17e-22 99.8
MS.gene23957.t1 MTR_7g110710 25.761 427 244 17 15 384 42 452 2.23e-22 99.0
MS.gene23957.t1 MTR_7g110710 25.761 427 244 17 15 384 42 452 2.43e-22 98.6
MS.gene23957.t1 MTR_1g100050 25.316 395 254 11 20 385 54 436 2.14e-21 95.9
MS.gene23957.t1 MTR_1g099850 25.377 398 250 11 20 385 59 441 2.39e-21 95.9
MS.gene23957.t1 MTR_1g100060 27.304 293 179 7 20 286 97 381 4.49e-21 95.1
MS.gene23957.t1 MTR_4g124980 25.245 408 236 14 16 380 74 455 4.53e-21 95.1
MS.gene23957.t1 MTR_8g098840 24.734 376 220 13 17 342 45 407 1.12e-20 94.0
MS.gene23957.t1 MTR_2g042740 25.000 404 242 13 16 380 56 437 1.40e-19 90.5
MS.gene23957.t1 MTR_8g028725 24.747 396 255 10 20 385 59 441 2.83e-19 89.7
MS.gene23957.t1 MTR_7g078680 27.575 301 174 10 17 286 56 343 5.32e-18 85.9
MS.gene23957.t1 MTR_3g449590 27.329 322 183 11 15 302 46 350 6.93e-18 85.5
MS.gene23957.t1 MTR_5g090160 25.688 327 187 12 13 302 41 348 1.51e-17 84.3
MS.gene23957.t1 MTR_1g086030 26.006 323 194 10 15 306 81 389 8.18e-17 82.4
MS.gene23957.t1 MTR_3g096330 27.301 326 169 14 18 300 78 378 9.09e-17 82.8
MS.gene23957.t1 MTR_1g086030 28.704 216 134 5 103 306 78 285 9.22e-16 78.6
MS.gene23957.t1 MTR_4g120730 24.513 359 215 12 15 336 39 378 7.52e-14 73.2
MS.gene23957.t1 MTR_7g072360 31.967 122 61 1 154 275 29 128 5.42e-11 60.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene23957.t1 AT5G17200 45.813 406 182 7 7 390 30 419 1.04e-113 338
MS.gene23957.t1 AT5G27530 43.257 393 199 3 1 390 38 409 6.43e-108 325
MS.gene23957.t1 AT4G35670 43.003 393 208 4 1 390 9 388 3.73e-103 310
MS.gene23957.t1 AT5G44840 40.256 390 224 4 1 390 9 389 1.98e-101 306
MS.gene23957.t1 AT3G15720 40.204 393 217 7 3 390 9 388 1.21e-96 296
MS.gene23957.t1 AT3G15720 40.204 393 217 7 3 390 10 389 1.24e-96 296
MS.gene23957.t1 AT3G15720 40.204 393 214 8 3 390 9 385 4.03e-95 292
MS.gene23957.t1 AT4G32380 39.578 379 207 4 1 377 24 382 1.90e-92 285
MS.gene23957.t1 AT1G80170 39.623 371 219 4 12 381 49 415 5.57e-92 283
MS.gene23957.t1 AT5G44830 40.974 349 187 3 42 390 1 330 2.59e-90 276
MS.gene23957.t1 AT1G56710 39.691 388 221 6 14 392 46 429 1.12e-89 277
MS.gene23957.t1 AT3G57510 41.899 358 198 7 14 368 66 416 3.79e-89 276
MS.gene23957.t1 AT1G70500 38.259 379 222 5 3 378 54 423 3.15e-86 270
MS.gene23957.t1 AT2G41850 41.598 363 202 7 2 361 54 409 1.22e-85 267
MS.gene23957.t1 AT1G23460 38.567 363 215 5 17 378 44 399 5.48e-84 263
MS.gene23957.t1 AT1G23460 38.567 363 215 5 17 378 68 423 1.30e-83 263
MS.gene23957.t1 AT5G14650 38.320 381 224 8 12 390 47 418 2.75e-81 256
MS.gene23957.t1 AT1G02460 37.209 387 230 8 16 391 89 473 4.96e-81 257
MS.gene23957.t1 AT3G07970 39.828 349 203 5 11 358 66 408 1.34e-80 254
MS.gene23957.t1 AT4G01890 36.896 393 233 9 10 390 59 448 2.52e-80 254
MS.gene23957.t1 AT1G48100 35.990 389 237 7 12 392 76 460 5.74e-80 254
MS.gene23957.t1 AT1G10640 35.961 406 226 11 13 390 116 515 3.09e-79 253
MS.gene23957.t1 AT1G60590 35.437 412 229 10 10 390 117 522 9.81e-79 252
MS.gene23957.t1 AT3G15720 38.988 336 189 5 59 390 2 325 6.94e-78 246
MS.gene23957.t1 AT4G32380 35.544 377 184 4 1 377 24 341 1.14e-76 243
MS.gene23957.t1 AT1G05660 36.641 393 231 10 2 390 14 392 3.91e-76 241
MS.gene23957.t1 AT1G05650 37.696 382 220 10 2 379 14 381 5.35e-76 241
MS.gene23957.t1 AT3G26610 35.484 372 227 6 16 379 67 433 1.00e-74 240
MS.gene23957.t1 AT2G43880 37.895 380 217 9 15 390 30 394 3.36e-72 231
MS.gene23957.t1 AT4G35670 43.686 293 149 4 101 390 2 281 4.31e-72 227
MS.gene23957.t1 AT4G32370 41.901 284 152 4 32 310 51 326 9.31e-72 228
MS.gene23957.t1 AT2G43890 37.571 354 206 9 1 350 12 354 4.02e-71 228
MS.gene23957.t1 AT1G02790 37.719 342 195 9 14 353 50 375 7.55e-69 223
MS.gene23957.t1 AT1G78400 37.067 375 218 8 10 378 29 391 7.75e-69 223
MS.gene23957.t1 AT2G43860 38.021 384 219 12 14 392 31 400 1.17e-67 219
MS.gene23957.t1 AT1G80140 36.176 340 205 4 54 390 6 336 2.48e-66 214
MS.gene23957.t1 AT1G43080 34.243 403 242 11 1 390 11 403 2.61e-66 216
MS.gene23957.t1 AT1G80170 42.322 267 149 4 12 277 49 311 3.45e-66 213
MS.gene23957.t1 AT4G32375 39.860 286 163 2 92 376 4 281 4.01e-66 216
MS.gene23957.t1 AT2G15460 35.829 374 220 11 16 380 31 393 6.75e-66 215
MS.gene23957.t1 AT1G65570 36.031 383 228 11 1 379 17 386 1.28e-65 214
MS.gene23957.t1 AT2G15450 35.561 374 221 11 16 380 31 393 2.30e-65 214
MS.gene23957.t1 AT1G43090 35.310 371 222 10 16 378 31 391 2.87e-65 214
MS.gene23957.t1 AT2G26620 34.771 371 226 9 16 380 31 391 4.61e-65 213
MS.gene23957.t1 AT2G40310 33.419 389 231 10 15 390 30 403 9.30e-65 212
MS.gene23957.t1 AT4G13760 36.127 346 202 10 16 353 31 365 1.15e-64 212
MS.gene23957.t1 AT5G39910 34.066 364 179 5 2 363 23 327 2.38e-64 210
MS.gene23957.t1 AT4G18180 35.028 354 220 6 11 360 34 381 5.52e-64 211
MS.gene23957.t1 AT1G43100 34.271 391 234 11 1 378 11 391 6.86e-64 211
MS.gene23957.t1 AT2G15470 35.294 374 222 11 16 380 31 393 1.11e-63 209
MS.gene23957.t1 AT1G17150 34.474 380 233 10 16 390 33 401 1.52e-63 209
MS.gene23957.t1 AT3G59850 37.069 348 204 9 15 358 22 358 2.27e-63 208
MS.gene23957.t1 AT3G59850 37.249 349 202 10 15 358 66 402 3.92e-63 209
MS.gene23957.t1 AT2G43870 34.091 396 238 14 1 390 6 384 1.05e-62 206
MS.gene23957.t1 AT2G33160 35.215 372 220 10 16 378 32 391 1.77e-62 212
MS.gene23957.t1 AT3G07820 35.347 331 201 8 34 361 38 358 1.39e-58 196
MS.gene23957.t1 AT5G14650 40.824 267 149 6 12 277 47 305 3.61e-58 192
MS.gene23957.t1 AT2G43880 39.000 300 172 7 15 313 30 319 1.01e-57 191
MS.gene23957.t1 AT5G48140 35.045 331 201 7 34 361 39 358 1.10e-57 193
MS.gene23957.t1 AT3G07830 32.609 368 221 9 34 387 39 393 8.92e-55 186
MS.gene23957.t1 AT3G07840 34.743 331 203 8 34 361 39 359 3.66e-53 182
MS.gene23957.t1 AT3G07850 35.065 385 224 10 9 387 63 427 4.17e-53 182
MS.gene23957.t1 AT3G14040 35.356 379 219 10 15 387 70 428 4.58e-53 182
MS.gene23957.t1 AT2G23900 25.735 408 237 16 20 385 60 443 7.58e-25 106
MS.gene23957.t1 AT3G62110 25.187 401 265 10 15 389 42 433 3.35e-24 104
MS.gene23957.t1 AT3G62110 25.187 401 265 10 15 389 42 433 3.35e-24 104
MS.gene23957.t1 AT3G42950 26.716 408 230 13 16 380 66 447 1.46e-23 102
MS.gene23957.t1 AT3G48950 26.899 316 173 12 20 294 49 347 1.84e-22 99.4
MS.gene23957.t1 AT3G61490 28.333 300 173 9 17 286 159 446 4.14e-22 98.6
MS.gene23957.t1 AT3G61490 28.333 300 173 9 17 286 61 348 5.31e-22 97.8
MS.gene23957.t1 AT3G61490 28.333 300 173 9 17 286 46 333 7.03e-22 97.4
MS.gene23957.t1 AT3G61490 28.333 300 173 9 17 286 46 333 7.03e-22 97.4
MS.gene23957.t1 AT3G61490 28.333 300 173 9 17 286 46 333 7.03e-22 97.4
MS.gene23957.t1 AT4G33440 29.063 320 175 12 16 300 72 374 3.20e-21 95.5
MS.gene23957.t1 AT4G23500 24.064 374 221 12 20 343 72 432 1.60e-18 87.4
MS.gene23957.t1 AT5G41870 26.101 318 191 8 16 302 45 349 1.06e-17 84.7
MS.gene23957.t1 AT4G23820 26.814 317 187 10 17 302 42 344 2.27e-17 84.0
MS.gene23957.t1 AT1G19170 24.623 398 252 14 16 380 87 469 5.23e-16 80.1
MS.gene23957.t1 AT3G16850 23.380 355 200 11 15 317 39 373 5.21e-12 67.4
MS.gene23957.t1 AT5G49215 23.962 313 184 11 18 294 43 337 6.39e-11 64.3
MS.gene23957.t1 AT5G49215 23.962 313 184 11 18 294 45 339 6.42e-11 64.3

Find 83 sgRNAs with CRISPR-Local

Find 203 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AACCAATCCATGTGTTTATT+TGG 0.080796 2.1:-2022233 None:intergenic
ATATCAATGCCATCAGTATT+AGG 0.225865 2.1:-2023052 None:intergenic
TGCTATCGATTTCAAATAAA+AGG 0.252766 2.1:+2022932 MS.gene23957:CDS
TTTGATTAACACAATAAAAT+TGG 0.261059 2.1:-2023826 None:intergenic
AACTCTAACAAGCTGCAAAA+AGG 0.263487 2.1:+2022988 MS.gene23957:CDS
ACGTTGACTAAAACATTAAC+TGG 0.308747 2.1:+2023511 MS.gene23957:CDS
ACCAATCCATGTGTTTATTT+GGG 0.310797 2.1:-2022232 None:intergenic
TAAAATTGGTCAATCATAAT+GGG 0.312360 2.1:-2023812 None:intergenic
GGTTAGAGCACCCCACATTT+TGG 0.323169 2.1:-2024073 None:intergenic
GGTCAATCATAATGGGGTTA+TGG 0.323679 2.1:-2023805 None:intergenic
ACTTTCAATGTATTGCAATA+TGG 0.324810 2.1:+2020820 MS.gene23957:CDS
TGCAGCTTGTTAGAGTTATA+TGG 0.325682 2.1:-2022982 None:intergenic
ATAAAATTGGTCAATCATAA+TGG 0.333850 2.1:-2023813 None:intergenic
AAGGAGAAAACCTTCATCTT+AGG 0.335531 2.1:-2024131 None:intergenic
AAAACAAAATCGGAATATTC+AGG 0.348846 2.1:+2022207 MS.gene23957:CDS
CATGGACCACGGAAAGCTAT+TGG 0.364550 2.1:-2021105 None:intergenic
CTTAACTCTTTCATTGCAAC+TGG 0.372034 2.1:+2023100 MS.gene23957:CDS
TTGAGCAAGCAGTTTAGAAT+TGG 0.374243 2.1:-2024191 None:intergenic
GTCAATCATAATGGGGTTAT+GGG 0.383263 2.1:-2023804 None:intergenic
AGGCTCTTCCATGAGCATTA+TGG 0.387155 2.1:-2024157 None:intergenic
TTAATTTACAGGAGAATCAT+TGG 0.387802 2.1:-2020863 None:intergenic
TATCAATGCCATCAGTATTA+GGG 0.410347 2.1:-2023051 None:intergenic
CAAGCAGTTTAGAATTGGTT+TGG 0.412319 2.1:-2024186 None:intergenic
AACATCCGTTTAATTGCTCC+TGG 0.419774 2.1:+2023022 MS.gene23957:CDS
AGAGTTATATGGCTTCTTGC+TGG 0.423641 2.1:-2022971 None:intergenic
GGTTTGATTATAAGTGGAAA+AGG 0.427621 2.1:+2022270 MS.gene23957:CDS
GTGGTCCAGGACATGGTATC+AGG 0.443137 2.1:+2023290 MS.gene23957:CDS
AGTGGAAAAGGGACTATTGA+TGG 0.444615 2.1:+2022282 MS.gene23957:CDS
ATGCACATCCTCTACAATAT+CGG 0.446275 2.1:-2023476 None:intergenic
TGTTGCACCTAAAACAAAAT+CGG 0.452223 2.1:+2022197 MS.gene23957:CDS
GTGAGTGATGTAACATATAG+AGG 0.462577 2.1:+2024002 MS.gene23957:CDS
GATGTGTGTCAAAGCAAATC+AGG 0.463712 2.1:+2021036 MS.gene23957:CDS
GTTTGATTATAAGTGGAAAA+GGG 0.469200 2.1:+2022271 MS.gene23957:CDS
TCCTTCTGCCATAATGCTCA+TGG 0.470413 2.1:+2024149 MS.gene23957:CDS
GTGAATGGTTTGATTATAAG+TGG 0.472070 2.1:+2022264 MS.gene23957:CDS
GATTGCATTGCTATTAGTGC+TGG 0.472202 2.1:+2023235 MS.gene23957:CDS
AGTAAGAATGTCTTGGCTGC+TGG 0.474123 2.1:-2021078 None:intergenic
ATTGGTTTCTCATTTGTGAA+TGG 0.475752 2.1:+2022249 MS.gene23957:CDS
ATCTGCTGTTCCTAAGATGA+AGG 0.477417 2.1:+2024121 MS.gene23957:CDS
ATTTCTGAACAGGGTGGAGG+TGG 0.479630 2.1:+2023737 MS.gene23957:intron
TCCCAAATAAACACATGGAT+TGG 0.484532 2.1:+2022231 MS.gene23957:CDS
TCTCTCCAGGAGCAATTAAA+CGG 0.494330 2.1:-2023027 None:intergenic
ATCTAATTATATTTACATTG+AGG 0.498845 2.1:+2021131 MS.gene23957:CDS
GTAACATATAGAGGGATTAG+TGG 0.503191 2.1:+2024011 MS.gene23957:CDS
GTATTGCAATATGGAGCAGT+TGG 0.517751 2.1:+2020829 MS.gene23957:CDS
TAAATATAATTAGATTTGCA+TGG 0.520032 2.1:-2021123 None:intergenic
CAATATGGAGCAGTTGGAGA+TGG 0.520589 2.1:+2020835 MS.gene23957:CDS
ACCAACCTGATACCATGTCC+TGG 0.526714 2.1:-2023295 None:intergenic
TGGCCTCAGTAAGAATGTCT+TGG 0.534716 2.1:-2021085 None:intergenic
TTTGAACTGCGACCAAAATG+TGG 0.542733 2.1:+2024061 MS.gene23957:CDS
TGTATTTCTGAACAGGGTGG+AGG 0.543516 2.1:+2023734 MS.gene23957:intron
AGTAAGAATCAAGACAAAGC+AGG 0.556906 2.1:+2023534 MS.gene23957:CDS
TCAGTATTAGGGCTCTCTCC+AGG 0.559038 2.1:-2023040 None:intergenic
CATAAACACGATCAAATACA+AGG 0.567940 2.1:-2024094 None:intergenic
TAGGTACTACTTACTGTTGG+TGG 0.572062 2.1:-2022771 None:intergenic
ATTCTAAGTATGTTTAAACT+CGG 0.572318 2.1:+2020781 MS.gene23957:CDS
ACTGCTATAACATGTGGTCC+AGG 0.572929 2.1:+2023277 MS.gene23957:CDS
TAGAATTGGTTTGGTGTGTG+AGG 0.574986 2.1:-2024177 None:intergenic
GGTGGTGGATTTCCTATGCA+AGG 0.577836 2.1:-2022330 None:intergenic
AGGGTGGAGGTGGATTTGCT+AGG 0.585416 2.1:+2023747 MS.gene23957:intron
GTGGAGGTGGATTTGCTAGG+AGG 0.587173 2.1:+2023750 MS.gene23957:CDS
AAAGGGACTATTGATGGTCG+AGG 0.592324 2.1:+2022288 MS.gene23957:CDS
GGAGAGAGCCCTAATACTGA+TGG 0.597997 2.1:+2023043 MS.gene23957:CDS
TCCATGAGCATTATGGCAGA+AGG 0.604867 2.1:-2024150 None:intergenic
ATAACATGTGGTCCAGGACA+TGG 0.613113 2.1:+2023283 MS.gene23957:CDS
TGAGTGATGTAACATATAGA+GGG 0.613324 2.1:+2024003 MS.gene23957:CDS
CTGAGGCCAATAGCTTTCCG+TGG 0.613352 2.1:+2021099 MS.gene23957:CDS
TGGTGGAAACAACCTTGCAT+AGG 0.613636 2.1:+2022318 MS.gene23957:CDS
CAGGATCCCAAATAAACACA+TGG 0.616392 2.1:+2022226 MS.gene23957:CDS
AATTAGATTTGCATGGACCA+CGG 0.624578 2.1:-2021116 None:intergenic
TACTACTTACTGTTGGTGGA+CGG 0.626963 2.1:-2022767 None:intergenic
AAAATAACTGCTATAACATG+TGG 0.629212 2.1:+2023271 MS.gene23957:CDS
TTGTAGCATTGGATCATTGG+GGG 0.630301 2.1:+2023441 MS.gene23957:intron
TTGAACTGCGACCAAAATGT+GGG 0.634447 2.1:+2024062 MS.gene23957:CDS
AAGCACTTGAATGTCTCTTG+AGG 0.634460 2.1:-2023080 None:intergenic
AAAATTGGTCAATCATAATG+GGG 0.641528 2.1:-2023811 None:intergenic
TTTGTAGCATTGGATCATTG+GGG 0.669066 2.1:+2023440 MS.gene23957:intron
TGGAGACACCGATATTGTAG+AGG 0.670467 2.1:+2023468 MS.gene23957:CDS
TGGAACATCATTAACAGACA+AGG 0.670604 2.1:+2024031 MS.gene23957:CDS
CAGCCAAGACATTCTTACTG+AGG 0.677474 2.1:+2021082 MS.gene23957:CDS
TTGATGGTCGAGGCTCTATG+TGG 0.678132 2.1:+2022298 MS.gene23957:CDS
ATGGTCGAGGCTCTATGTGG+TGG 0.681626 2.1:+2022301 MS.gene23957:CDS
TGAACTGCGACCAAAATGTG+GGG 0.756518 2.1:+2024063 MS.gene23957:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GGAAAAAATAAAATAAAATA+AGG + chr2.1:2021297-2021316 MS.gene23957:intron 10.0%
!! TACATATAAAAATTTACTTA+CGG - chr2.1:2022355-2022374 None:intergenic 10.0%
!!! AAATAAAATAAGGTATTAAA+AGG + chr2.1:2021307-2021326 MS.gene23957:intron 10.0%
!!! TTATATGTATAGTTTTTTTA+AGG + chr2.1:2022366-2022385 MS.gene23957:intron 10.0%
!! AAAAATACTTTGGTAAATTA+AGG - chr2.1:2021852-2021871 None:intergenic 15.0%
!! AATAAAGAAATTAATACTCA+TGG - chr2.1:2023605-2023624 None:intergenic 15.0%
!! ACATATAAAAATTTACTTAC+GGG - chr2.1:2022354-2022373 None:intergenic 15.0%
!! AGCATATACTTTAAATTATT+AGG - chr2.1:2020977-2020996 None:intergenic 15.0%
!! AGTAACATATATAAGAAAAA+TGG - chr2.1:2023896-2023915 None:intergenic 15.0%
!! ATATCCATTTAAAAATACTT+TGG - chr2.1:2021862-2021881 None:intergenic 15.0%
!! ATCTAATTATATTTACATTG+AGG + chr2.1:2021131-2021150 MS.gene23957:CDS 15.0%
!! ATTGTATCTAATCATATTAT+AGG + chr2.1:2023208-2023227 MS.gene23957:intron 15.0%
!! GATTTAACAACAAAAAAAAA+TGG + chr2.1:2021903-2021922 MS.gene23957:intron 15.0%
!! GTTGTAGAAAATTAATTAAT+CGG - chr2.1:2023929-2023948 None:intergenic 15.0%
!! TAAAATAAAATAATCCACAA+AGG + chr2.1:2022082-2022101 MS.gene23957:intron 15.0%
!! TAAATATAATTAGATTTGCA+TGG - chr2.1:2021126-2021145 None:intergenic 15.0%
!! TTTGATTAACACAATAAAAT+TGG - chr2.1:2023829-2023848 None:intergenic 15.0%
!!! ATATATTATTTTTGTAGCAT+TGG + chr2.1:2023430-2023449 MS.gene23957:intron 15.0%
!!! ATATTTTTTTTTCCTTTTTC+AGG + chr2.1:2022734-2022753 MS.gene23957:intron 15.0%
!!! ATTTTATTGTGTTAATCAAA+TGG + chr2.1:2023829-2023848 MS.gene23957:CDS 15.0%
!!! TATTTTTTTTTCCTTTTTCA+GGG + chr2.1:2022735-2022754 MS.gene23957:intron 15.0%
!!! TGCTATAATATTTTTTCAAT+AGG + chr2.1:2020993-2021012 MS.gene23957:intron 15.0%
!!! TTTACCAAAGTATTTTTAAA+TGG + chr2.1:2021855-2021874 MS.gene23957:intron 15.0%
!!! TTTCACTTATACTATAATTT+TGG + chr2.1:2023651-2023670 MS.gene23957:intron 15.0%
!!! TTTTTTTTGTTGTTAAATCA+AGG - chr2.1:2021902-2021921 None:intergenic 15.0%
!! AAAATAAATACCAGTTCATA+TGG - chr2.1:2022873-2022892 None:intergenic 20.0%
!! AATAACGAAATACTTTACAA+TGG + chr2.1:2022046-2022065 MS.gene23957:intron 20.0%
!! ACCAAAATAACTAACATTAT+TGG + chr2.1:2023570-2023589 MS.gene23957:intron 20.0%
!! ATAAAATTGGTCAATCATAA+TGG - chr2.1:2023816-2023835 None:intergenic 20.0%
!! ATAAGTGAAAAACCTTATAT+AGG - chr2.1:2023641-2023660 None:intergenic 20.0%
!! ATTAATGTGAAGATCAATTA+AGG + chr2.1:2021584-2021603 MS.gene23957:intron 20.0%
!! ATTCTAAGTATGTTTAAACT+CGG + chr2.1:2020781-2020800 MS.gene23957:CDS 20.0%
!! CAAGTAGTAAAATTCATTAA+AGG + chr2.1:2022019-2022038 MS.gene23957:intron 20.0%
!! GAAAAAACATATGTTATAAG+AGG + chr2.1:2021789-2021808 MS.gene23957:intron 20.0%
!! TAAAATTGGTCAATCATAAT+GGG - chr2.1:2023815-2023834 None:intergenic 20.0%
!! TGAAAGGTTAAGTTAAAAAA+AGG - chr2.1:2022653-2022672 None:intergenic 20.0%
!! TTAATGTGAAGATCAATTAA+GGG + chr2.1:2021585-2021604 MS.gene23957:intron 20.0%
!! TTAATTGATCTTCACATTAA+TGG - chr2.1:2021585-2021604 None:intergenic 20.0%
!! TTCATTAAAAGTGAATACTT+AGG - chr2.1:2021392-2021411 None:intergenic 20.0%
!!! AAAATAAGGTATTAAAAGGA+TGG + chr2.1:2021311-2021330 MS.gene23957:intron 20.0%
!!! ACCAATAATGTTAGTTATTT+TGG - chr2.1:2023574-2023593 None:intergenic 20.0%
!!! ATTTTTTTTTCCTTTTTCAG+GGG + chr2.1:2022736-2022755 MS.gene23957:intron 20.0%
!!! TAAGAGGTCAAATTTTTAAA+TGG + chr2.1:2021805-2021824 MS.gene23957:intron 20.0%
!!! TATTTATTTTTGTGTGTGTA+TGG + chr2.1:2022883-2022902 MS.gene23957:intron 20.0%
!!! TATTTTATTTTTTCCGTTGA+AGG - chr2.1:2021292-2021311 None:intergenic 20.0%
!!! TTTAAGTAACATAAGTTGAA+AGG - chr2.1:2022669-2022688 None:intergenic 20.0%
! AAAAATGGTATAACAATCCA+TGG + chr2.1:2021918-2021937 MS.gene23957:intron 25.0%
! AAAACAAAATCGGAATATTC+AGG + chr2.1:2022207-2022226 MS.gene23957:CDS 25.0%
! AAAATAACTGCTATAACATG+TGG + chr2.1:2023271-2023290 MS.gene23957:CDS 25.0%
! AAAATTGGTCAATCATAATG+GGG - chr2.1:2023814-2023833 None:intergenic 25.0%
! AATACTTAGGATCAATGATA+TGG - chr2.1:2021379-2021398 None:intergenic 25.0%
! ACTTTCAATGTATTGCAATA+TGG + chr2.1:2020820-2020839 MS.gene23957:CDS 25.0%
! AGAGATCATAGTTAATTTAC+AGG - chr2.1:2020877-2020896 None:intergenic 25.0%
! ATGATAACATCAAAGATTGA+TGG - chr2.1:2021944-2021963 None:intergenic 25.0%
! ATGTAAAGTTGCTAGATTAA+TGG - chr2.1:2023397-2023416 None:intergenic 25.0%
! GTTTGATTATAAGTGGAAAA+GGG + chr2.1:2022271-2022290 MS.gene23957:CDS 25.0%
! TATAAAAATTTACTTACGGG+TGG - chr2.1:2022351-2022370 None:intergenic 25.0%
! TGATGCAAGTATTACTATAA+AGG - chr2.1:2022706-2022725 None:intergenic 25.0%
! TTAATTTACAGGAGAATCAT+TGG - chr2.1:2020866-2020885 None:intergenic 25.0%
!! ATAAGTCCTTTAAGTTTCTA+AGG - chr2.1:2022566-2022585 None:intergenic 25.0%
!! ATATGTCCTTTAAGTTTCTA+AGG + chr2.1:2022461-2022480 MS.gene23957:intron 25.0%
!! CTGTAACATTTGAAACTTAA+AGG + chr2.1:2022586-2022605 MS.gene23957:intron 25.0%
!! GAATTTTACTACTTGTCTAA+AGG - chr2.1:2022014-2022033 None:intergenic 25.0%
!! TCTATAGTATGTATTTTGCA+AGG - chr2.1:2022810-2022829 None:intergenic 25.0%
!! TTCAATTGTATTTCTGAACA+GGG + chr2.1:2023728-2023747 MS.gene23957:intron 25.0%
!! TTTCAATTGTATTTCTGAAC+AGG + chr2.1:2023727-2023746 MS.gene23957:intron 25.0%
!!! GAATATTCCGATTTTGTTTT+AGG - chr2.1:2022207-2022226 None:intergenic 25.0%
!!! GTTGATATGTTACTTTGTTT+AGG + chr2.1:2021513-2021532 MS.gene23957:intron 25.0%
!!! TGCTATCGATTTCAAATAAA+AGG + chr2.1:2022932-2022951 MS.gene23957:CDS 25.0%
AAAGTAACATATCAACCACA+AGG - chr2.1:2021509-2021528 None:intergenic 30.0%
AACCAATCCATGTGTTTATT+TGG - chr2.1:2022236-2022255 None:intergenic 30.0%
AACTTAAGACATTGCATAAG+CGG + chr2.1:2021452-2021471 MS.gene23957:intron 30.0%
ACCAATCCATGTGTTTATTT+GGG - chr2.1:2022235-2022254 None:intergenic 30.0%
ATATCAATGCCATCAGTATT+AGG - chr2.1:2023055-2023074 None:intergenic 30.0%
ATTGATCTTCACATTAATGG+TGG - chr2.1:2021582-2021601 None:intergenic 30.0%
CATAAACACGATCAAATACA+AGG - chr2.1:2024097-2024116 None:intergenic 30.0%
CTGAAACCTTAGAAACTTAA+AGG + chr2.1:2022557-2022576 MS.gene23957:intron 30.0%
CTGAAACCTTAGAAACTTAA+AGG - chr2.1:2022576-2022557 None:intergenic 30.0%
GAAACAAACTCGAAACTTAA+AGG - chr2.1:2022499-2022518 None:intergenic 30.0%
GGTTTGATTATAAGTGGAAA+AGG + chr2.1:2022270-2022289 MS.gene23957:CDS 30.0%
GTGAATGGTTTGATTATAAG+TGG + chr2.1:2022264-2022283 MS.gene23957:CDS 30.0%
GTTACAAACGTGAAACTTAA+AGG - chr2.1:2022410-2022429 None:intergenic 30.0%
GTTACAAACGTGAAAGTTAA+AGG + chr2.1:2022617-2022636 MS.gene23957:intron 30.0%
TATCAATGCCATCAGTATTA+GGG - chr2.1:2023054-2023073 None:intergenic 30.0%
TGAAAGTTAAAGGACGTATT+TGG + chr2.1:2022627-2022646 MS.gene23957:intron 30.0%
TGAGTGATGTAACATATAGA+GGG + chr2.1:2024003-2024022 MS.gene23957:CDS 30.0%
TGTTGCACCTAAAACAAAAT+CGG + chr2.1:2022197-2022216 MS.gene23957:CDS 30.0%
TTCAACCATAATTCCTTCAA+CGG + chr2.1:2021276-2021295 MS.gene23957:intron 30.0%
! ACCTCATCAAGTGAAAATTT+TGG + chr2.1:2021191-2021210 MS.gene23957:intron 30.0%
! ACGTTGACTAAAACATTAAC+TGG + chr2.1:2023511-2023530 MS.gene23957:CDS 30.0%
! ATAGGTCCTTTAAGTTTCAA+AGG + chr2.1:2022432-2022451 MS.gene23957:intron 30.0%
! ATTGGTTTCTCATTTGTGAA+TGG + chr2.1:2022249-2022268 MS.gene23957:CDS 30.0%
! TCCAAAATTTTCACTTGATG+AGG - chr2.1:2021195-2021214 None:intergenic 30.0%
! TTTTTCCGTTGAAGGAATTA+TGG - chr2.1:2021284-2021303 None:intergenic 30.0%
!! AAGTTTCTAAGGTTTCAGAT+AGG + chr2.1:2022472-2022491 MS.gene23957:intron 30.0%
!! AAGTTTCTAAGGTTTCAGAT+AGG - chr2.1:2022491-2022472 None:intergenic 30.0%
!! CTGTAACCTTTGAAACTTAA+AGG - chr2.1:2022441-2022460 None:intergenic 30.0%
!! TAATTTTGGTCACATGACTT+AGG + chr2.1:2023665-2023684 MS.gene23957:intron 30.0%
!! TTTTGTAGCATTGGATCATT+GGG + chr2.1:2023439-2023458 MS.gene23957:intron 30.0%
!! TTTTTGTAGCATTGGATCAT+TGG + chr2.1:2023438-2023457 MS.gene23957:intron 30.0%
!!! CAAGTGAAAATTTTGGAACT+TGG + chr2.1:2021198-2021217 MS.gene23957:intron 30.0%
!!! TGAAAATTTTGGAACTTGGT+CGG + chr2.1:2021202-2021221 MS.gene23957:intron 30.0%
AAAAATTTACTTACGGGTGG+TGG - chr2.1:2022348-2022367 None:intergenic 35.0%
AACTCTAACAAGCTGCAAAA+AGG + chr2.1:2022988-2023007 MS.gene23957:CDS 35.0%
AAGGAGAAAACCTTCATCTT+AGG - chr2.1:2024134-2024153 None:intergenic 35.0%
AATTAGATTTGCATGGACCA+CGG - chr2.1:2021119-2021138 None:intergenic 35.0%
ACTCTAACAAGCTGCAAAAA+GGG + chr2.1:2022989-2023008 MS.gene23957:CDS 35.0%
AGTAAGAATCAAGACAAAGC+AGG + chr2.1:2023534-2023553 MS.gene23957:CDS 35.0%
ATGCACATCCTCTACAATAT+CGG - chr2.1:2023479-2023498 None:intergenic 35.0%
CGTACTACTACTCCTATATA+AGG + chr2.1:2023626-2023645 MS.gene23957:intron 35.0%
CTTAACTCTTTCATTGCAAC+TGG + chr2.1:2023100-2023119 MS.gene23957:CDS 35.0%
CTTTCACGTTTGTAACAGAT+AGG - chr2.1:2022613-2022632 None:intergenic 35.0%
GTAACATATAGAGGGATTAG+TGG + chr2.1:2024011-2024030 MS.gene23957:CDS 35.0%
GTGAGTGATGTAACATATAG+AGG + chr2.1:2024002-2024021 MS.gene23957:CDS 35.0%
GTTTCACGTTTGTAACAGAT+AGG + chr2.1:2022414-2022433 MS.gene23957:intron 35.0%
TATCAACCACAAGGTTACAT+TGG - chr2.1:2021500-2021519 None:intergenic 35.0%
TCAAATGGTCTGCAAAAACA+TGG + chr2.1:2023844-2023863 MS.gene23957:CDS 35.0%
TCCCAAATAAACACATGGAT+TGG + chr2.1:2022231-2022250 MS.gene23957:CDS 35.0%
TGGAACATCATTAACAGACA+AGG + chr2.1:2024031-2024050 MS.gene23957:CDS 35.0%
TGTCTTAAGTTCAAACGTTG+TGG - chr2.1:2021443-2021462 None:intergenic 35.0%
TTGTGTGTGTATGGATGTAT+AGG + chr2.1:2022892-2022911 MS.gene23957:intron 35.0%
! AATTGTATTTCTGAACAGGG+TGG + chr2.1:2023731-2023750 MS.gene23957:intron 35.0%
! CAAGCAGTTTAGAATTGGTT+TGG - chr2.1:2024189-2024208 None:intergenic 35.0%
! GTCAATCATAATGGGGTTAT+GGG - chr2.1:2023807-2023826 None:intergenic 35.0%
! TTGAGCAAGCAGTTTAGAAT+TGG - chr2.1:2024194-2024213 None:intergenic 35.0%
! TTTGTAGCATTGGATCATTG+GGG + chr2.1:2023440-2023459 MS.gene23957:intron 35.0%
! TTTTGTGAACTCAGCTTTCA+GGG + chr2.1:2022169-2022188 MS.gene23957:intron 35.0%
!! AATAGGCTTTTCTGAAAGCA+TGG + chr2.1:2021010-2021029 MS.gene23957:intron 35.0%
!! GTTTCGAGTTTGTTTCAGAT+AGG + chr2.1:2022503-2022522 MS.gene23957:intron 35.0%
!! TGCAGCTTGTTAGAGTTATA+TGG - chr2.1:2022985-2023004 None:intergenic 35.0%
!!! ACATGCAGAAGAGTATTTTG+AGG - chr2.1:2020918-2020937 None:intergenic 35.0%
AACATCCGTTTAATTGCTCC+TGG + chr2.1:2023022-2023041 MS.gene23957:CDS 40.0%
AAGCACTTGAATGTCTCTTG+AGG - chr2.1:2023083-2023102 None:intergenic 40.0%
AGAAATGTGGTTGAGAGAAG+AGG - chr2.1:2021415-2021434 None:intergenic 40.0%
AGAGTTATATGGCTTCTTGC+TGG - chr2.1:2022974-2022993 None:intergenic 40.0%
AGTGGAAAAGGGACTATTGA+TGG + chr2.1:2022282-2022301 MS.gene23957:CDS 40.0%
ATCTGCTGTTCCTAAGATGA+AGG + chr2.1:2024121-2024140 MS.gene23957:CDS 40.0%
CAGGATCCCAAATAAACACA+TGG + chr2.1:2022226-2022245 MS.gene23957:CDS 40.0%
CGTTGTGGATGAAAGAAATG+TGG - chr2.1:2021428-2021447 None:intergenic 40.0%
CTGTTACGTACCATATGAAC+TGG + chr2.1:2022860-2022879 MS.gene23957:intron 40.0%
CTTTCTATCAACCTCCGTTA+AGG + chr2.1:2022528-2022547 MS.gene23957:intron 40.0%
GAGTAGGTACTACTTACTGT+TGG - chr2.1:2022777-2022796 None:intergenic 40.0%
GATGTGTGTCAAAGCAAATC+AGG + chr2.1:2021036-2021055 MS.gene23957:CDS 40.0%
GCAAGGATCAATACAAGAGT+AGG - chr2.1:2022793-2022812 None:intergenic 40.0%
GGTCTGAGTAGAACTATACT+TGG - chr2.1:2021479-2021498 None:intergenic 40.0%
GGTTTCAGATAGGTCCTTAA+CGG - chr2.1:2022545-2022564 None:intergenic 40.0%
GTATTGCAATATGGAGCAGT+TGG + chr2.1:2020829-2020848 MS.gene23957:CDS 40.0%
TAGGTACTACTTACTGTTGG+TGG - chr2.1:2022774-2022793 None:intergenic 40.0%
TCTCTCCAGGAGCAATTAAA+CGG - chr2.1:2023030-2023049 None:intergenic 40.0%
TTGAACTGCGACCAAAATGT+GGG + chr2.1:2024062-2024081 MS.gene23957:CDS 40.0%
TTTGAACTGCGACCAAAATG+TGG + chr2.1:2024061-2024080 MS.gene23957:CDS 40.0%
! ACATCAAAGATTGATGGCCA+TGG - chr2.1:2021938-2021957 None:intergenic 40.0%
! ACATGATAGTGTGACACAAG+TGG + chr2.1:2021707-2021726 MS.gene23957:intron 40.0%
! CTTTTGTGAACTCAGCTTTC+AGG + chr2.1:2022168-2022187 MS.gene23957:intron 40.0%
! GATTGCATTGCTATTAGTGC+TGG + chr2.1:2023235-2023254 MS.gene23957:CDS 40.0%
! GGTAAATTAAGGCATCTTCG+AGG - chr2.1:2021841-2021860 None:intergenic 40.0%
! GGTCAATCATAATGGGGTTA+TGG - chr2.1:2023808-2023827 None:intergenic 40.0%
! TACTACTTACTGTTGGTGGA+CGG - chr2.1:2022770-2022789 None:intergenic 40.0%
! TTGTAGCATTGGATCATTGG+GGG + chr2.1:2023441-2023460 MS.gene23957:intron 40.0%
!! CTTAACGGAGGTTGATAGAA+AGG - chr2.1:2022530-2022549 None:intergenic 40.0%
!! TAGAATTGGTTTGGTGTGTG+AGG - chr2.1:2024180-2024199 None:intergenic 40.0%
!!! AAATTTTGGAACTTGGTCGG+TGG + chr2.1:2021205-2021224 MS.gene23957:intron 40.0%
ACGTGAAACTTAAAGGACCC+AGG - chr2.1:2022403-2022422 None:intergenic 45.0%
ACTGCTATAACATGTGGTCC+AGG + chr2.1:2023277-2023296 MS.gene23957:CDS 45.0%
AGGCTCTTCCATGAGCATTA+TGG - chr2.1:2024160-2024179 None:intergenic 45.0%
AGTAAGAATGTCTTGGCTGC+TGG - chr2.1:2021081-2021100 None:intergenic 45.0%
ATAACATGTGGTCCAGGACA+TGG + chr2.1:2023283-2023302 MS.gene23957:CDS 45.0%
CAATATGGAGCAGTTGGAGA+TGG + chr2.1:2020835-2020854 MS.gene23957:CDS 45.0%
CAGCCAAGACATTCTTACTG+AGG + chr2.1:2021082-2021101 MS.gene23957:CDS 45.0%
CGTGAAACTTAAAGGACCCA+GGG - chr2.1:2022402-2022421 None:intergenic 45.0%
CTAGAGACTGTTCTCCTTTG+TGG - chr2.1:2022099-2022118 None:intergenic 45.0%
CTCAGACCAATGTAACCTTG+TGG + chr2.1:2021491-2021510 MS.gene23957:intron 45.0%
GATAGTGTGACACAAGTGGT+GGG + chr2.1:2021711-2021730 MS.gene23957:intron 45.0%
TCCATGAGCATTATGGCAGA+AGG - chr2.1:2024153-2024172 None:intergenic 45.0%
TCCTTCTGCCATAATGCTCA+TGG + chr2.1:2024149-2024168 MS.gene23957:CDS 45.0%
TGAACTGCGACCAAAATGTG+GGG + chr2.1:2024063-2024082 MS.gene23957:CDS 45.0%
TGGAGACACCGATATTGTAG+AGG + chr2.1:2023468-2023487 MS.gene23957:CDS 45.0%
TGGCCTCAGTAAGAATGTCT+TGG - chr2.1:2021088-2021107 None:intergenic 45.0%
TGGTGGAAACAACCTTGCAT+AGG + chr2.1:2022318-2022337 MS.gene23957:CDS 45.0%
TTAAGGCCAAATACCTCCCT+GGG + chr2.1:2022383-2022402 MS.gene23957:intron 45.0%
TTCAGATAGGTCCTTAACGG+AGG - chr2.1:2022542-2022561 None:intergenic 45.0%
! AAAGGGACTATTGATGGTCG+AGG + chr2.1:2022288-2022307 MS.gene23957:CDS 45.0%
! TGATAGTGTGACACAAGTGG+TGG + chr2.1:2021710-2021729 MS.gene23957:intron 45.0%
! TGTATTTCTGAACAGGGTGG+AGG + chr2.1:2023734-2023753 MS.gene23957:intron 45.0%
! TTTAAGGCCAAATACCTCCC+TGG + chr2.1:2022382-2022401 MS.gene23957:intron 45.0%
ACCAACCTGATACCATGTCC+TGG - chr2.1:2023298-2023317 None:intergenic 50.0%
ATTTCTGAACAGGGTGGAGG+TGG + chr2.1:2023737-2023756 MS.gene23957:intron 50.0%
CATGGACCACGGAAAGCTAT+TGG - chr2.1:2021108-2021127 None:intergenic 50.0%
GAAACTTAAAGGACCCAGGG+AGG - chr2.1:2022399-2022418 None:intergenic 50.0%
GGAGAGAGCCCTAATACTGA+TGG + chr2.1:2023043-2023062 MS.gene23957:CDS 50.0%
GGCAACTTGTCCCCTGAAAA+AGG - chr2.1:2022749-2022768 None:intergenic 50.0%
TCAGTATTAGGGCTCTCTCC+AGG - chr2.1:2023043-2023062 None:intergenic 50.0%
TCCAGGACATGGTATCAGGT+TGG + chr2.1:2023294-2023313 MS.gene23957:intron 50.0%
! TTGATGGTCGAGGCTCTATG+TGG + chr2.1:2022298-2022317 MS.gene23957:CDS 50.0%
!! AAAGGACCCAGGGAGGTATT+TGG - chr2.1:2022392-2022411 None:intergenic 50.0%
!! GGTGGTGGATTTCCTATGCA+AGG - chr2.1:2022333-2022352 None:intergenic 50.0%
!! GGTTAGAGCACCCCACATTT+TGG - chr2.1:2024076-2024095 None:intergenic 50.0%
ATGGTCGAGGCTCTATGTGG+TGG + chr2.1:2022301-2022320 MS.gene23957:CDS 55.0%
CTGAGGCCAATAGCTTTCCG+TGG + chr2.1:2021099-2021118 MS.gene23957:CDS 55.0%
GTGGTCCAGGACATGGTATC+AGG + chr2.1:2023290-2023309 MS.gene23957:CDS 55.0%
! AGGGTGGAGGTGGATTTGCT+AGG + chr2.1:2023747-2023766 MS.gene23957:intron 55.0%
! ATTGGATCATTGGGGGCACG+TGG + chr2.1:2023448-2023467 MS.gene23957:CDS 55.0%
!! GTGGAGGTGGATTTGCTAGG+AGG + chr2.1:2023750-2023769 MS.gene23957:CDS 55.0%


Chromosome Type Strat End Strand Name
chr2.1 gene 2020778 2024217 2020778 ID=MS.gene23957
chr2.1 mRNA 2020778 2024217 2020778 ID=MS.gene23957.t1;Parent=MS.gene23957
chr2.1 exon 2020778 2020876 2020778 ID=MS.gene23957.t1.exon1;Parent=MS.gene23957.t1
chr2.1 CDS 2020778 2020876 2020778 ID=cds.MS.gene23957.t1;Parent=MS.gene23957.t1
chr2.1 exon 2021015 2021152 2021015 ID=MS.gene23957.t1.exon2;Parent=MS.gene23957.t1
chr2.1 CDS 2021015 2021152 2021015 ID=cds.MS.gene23957.t1;Parent=MS.gene23957.t1
chr2.1 exon 2022183 2022353 2022183 ID=MS.gene23957.t1.exon3;Parent=MS.gene23957.t1
chr2.1 CDS 2022183 2022353 2022183 ID=cds.MS.gene23957.t1;Parent=MS.gene23957.t1
chr2.1 exon 2022756 2022779 2022756 ID=MS.gene23957.t1.exon4;Parent=MS.gene23957.t1
chr2.1 CDS 2022756 2022779 2022756 ID=cds.MS.gene23957.t1;Parent=MS.gene23957.t1
chr2.1 exon 2022914 2023121 2022914 ID=MS.gene23957.t1.exon5;Parent=MS.gene23957.t1
chr2.1 CDS 2022914 2023121 2022914 ID=cds.MS.gene23957.t1;Parent=MS.gene23957.t1
chr2.1 exon 2023230 2023311 2023230 ID=MS.gene23957.t1.exon6;Parent=MS.gene23957.t1
chr2.1 CDS 2023230 2023311 2023230 ID=cds.MS.gene23957.t1;Parent=MS.gene23957.t1
chr2.1 exon 2023447 2023555 2023447 ID=MS.gene23957.t1.exon7;Parent=MS.gene23957.t1
chr2.1 CDS 2023447 2023555 2023447 ID=cds.MS.gene23957.t1;Parent=MS.gene23957.t1
chr2.1 exon 2023749 2023865 2023749 ID=MS.gene23957.t1.exon8;Parent=MS.gene23957.t1
chr2.1 CDS 2023749 2023865 2023749 ID=cds.MS.gene23957.t1;Parent=MS.gene23957.t1
chr2.1 exon 2023987 2024217 2023987 ID=MS.gene23957.t1.exon9;Parent=MS.gene23957.t1
chr2.1 CDS 2023987 2024217 2023987 ID=cds.MS.gene23957.t1;Parent=MS.gene23957.t1
Gene Sequence

>MS.gene23957

ATGATTCTAAGTATGTTTAAACTCGGATTGAGTAGCACAACAACTTTCAATGTATTGCAATATGGAGCAGTTGGAGATGGAAAAACCAATGATTCTCCTGTAAATTAACTATGATCTCTCATCATTTTATTTAGTTGCCTCAAAATACTCTTCTGCATGTGTAACATTAATAACTTATTATTTCATTTGCTCAATTCCTAATAATTTAAAGTATATGCTATAATATTTTTTCAATAGGCTTTTCTGAAAGCATGGAAAGATGTGTGTCAAAGCAAATCAGGCACATCTCGTCTCATCATACCAGCAGCCAAGACATTCTTACTGAGGCCAATAGCTTTCCGTGGTCCATGCAAATCTAATTATATTTACATTGAGGTACTTCTTAATTAATTTGATTATTTTTGCAATACATAACCTCATCAAGTGAAAATTTTGGAACTTGGTCGGTGGCTAAAACTTAAAAGCATAGTAAACATTAACTTCTGTGTTGTTTCAACTTTCAACCATAATTCCTTCAACGGAAAAAATAAAATAAAATAAGGTATTAAAAGGATGGATACATATTTGATAAAATTGTTGTTATATTTTTTTTTTATAACCATATCATTGATCCTAAGTATTCACTTTTAATGAACCTCTTCTCTCAACCACATTTCTTTCATCCACAACGTTTGAACTTAAGACATTGCATAAGCGGTCCAAGTATAGTTCTACTCAGACCAATGTAACCTTGTGGTTGATATGTTACTTTGTTTAGGATAAGTATTCTATTTAAAGAAAATTGAATTAAGAAAAATTTCACCACCATTAATGTGAAGATCAATTAAGGGAATGAATTTTAAGTTTATAACAAATCATTTTTGTAATAAAAAAAAAAGTTTAACATAGTCATAATTTGCTATATATGAATGAATAAGTGATATCAACAAACATGATAGTGTGACACAAGTGGTGGGATGTTTTGATCTATTAACTATTTGATCTATGATTCAATCGTTTGTCGATGTTTATGAAAAAACATATGTTATAAGAGGTCAAATTTTTAAATGGATATCAATTACCTCGAAGATGCCTTAATTTACCAAAGTATTTTTAAATGGATATCAATTACTTCGAAGATACCTTGATTTAACAACAAAAAAAAATGGTATAACAATCCATGGCCATCAATCTTTGATGTTATCATCATTGAGTTTTTCATTTTTAGTACTTTTCATCAAAAGTCGAAGCTTGCCTTTAGACAAGTAGTAAAATTCATTAAAGGTACGAATAACGAAATACTTTACAATGGAATAAAAAAAGAATAAAATAAAATAATCCACAAAGGAGAACAGTCTCTAGTTGACTAGTATTTTAATATAATATTTGAGTTAAAGTAATCTATTTAGTACTTTTGTGAACTCAGCTTTCAGGGAATATTGTTGCACCTAAAACAAAATCGGAATATTCAGGATCCCAAATAAACACATGGATTGGTTTCTCATTTGTGAATGGTTTGATTATAAGTGGAAAAGGGACTATTGATGGTCGAGGCTCTATGTGGTGGAAACAACCTTGCATAGGAAATCCACCACCCGTAAGTAAATTTTTATATGTATAGTTTTTTTAAGGCCAAATACCTCCCTGGGTCCTTTAAGTTTCACGTTTGTAACAGATAGGTCCTTTAAGTTTCAAAGGTTACAGATATGTCCTTTAAGTTTCTAAGGTTTCAGATAGGTCCTTTAAGTTTCGAGTTTGTTTCAGATAGGTCCTTTCTATCAACCTCCGTTAAGGACCTATCTGAAACCTTAGAAACTTAAAGGACTTATCTGTAACATTTGAAACTTAAAGGACCTATCTGTTACAAACGTGAAAGTTAAAGGACGTATTTGGCCTTTTTTTAACTTAACCTTTCAACTTATGTTACTTAAATAATACACTATATACCTTTATAGTAATACTTGCATCATTTTTATGATATTTTTTTTTCCTTTTTCAGGGGACAAGTTGCCGTCCACCAACAGTAAGTAGTACCTACTCTTGTATTGATCCTTGCAAAATACATACTATAGACAATATTTTTGTTACAAAATTAAATTTTAGCTGTTACGTACCATATGAACTGGTATTTATTTTTGTGTGTGTATGGATGTATAGGCAATAACATTGAATAGATGCTATCGATTTCAAATAAAAGGATATACAAGTGTTAATCCAGCAAGAAGCCATATAACTCTAACAAGCTGCAAAAAGGGCATTATCTCTAACATCCGTTTAATTGCTCCTGGAGAGAGCCCTAATACTGATGGCATTGATATTTCTGCCTCAAGAGACATTCAAGTGCTTAACTCTTTCATTGCAACTGGTAATAATGTTTTAATAATTTGAACTCATTTCAATCTATATTAATTATTAGATCATCTTAGTGTATGTAAATAATCATATATATTGATTGTATCTAATCATATTATAGGTGATGATTGCATTGCTATTAGTGCTGGATCATCAGTAATCAAAATAACTGCTATAACATGTGGTCCAGGACATGGTATCAGGTTGGTAGTACTTCACTAAATATGTACTAATTGATCTGAACTTATAAAATATGATCTAATTGATCTGAACTAATTATATCATCCATTAATCTAGCAACTTTACATTAATTAAGAAAATATATATTATTTTTGTAGCATTGGATCATTGGGGGCACGTGGAGACACCGATATTGTAGAGGATGTGCATGTGAAGAATTGTACGTTGACTAAAACATTAACTGGAGTAAGAATCAAGACAAAGCAGGTAGTAATTAATTCACCAAAATAACTAACATTATTGGTTACAAACTCCATGAGTATTAATTTCTTTATTACGTACTACTACTCCTATATAAGGTTTTTCACTTATACTATAATTTTGGTCACATGACTTAGGTAAAATATTTTATTTTAAATTTTAAATGTAATCATTCAATTTCAATTGTATTTCTGAACAGGGTGGAGGTGGATTTGCTAGGAGGATCACGTTTGAAAATATTAGATTTGTTCGAGCCCATAACCCCATTATGATTGACCAATTTTATTGTGTTAATCAAATGGTCTGCAAAAACATGGTAATAACTCAAGATAATGCATCAATTCCATTTTTCTTATATATGTTACTTTCTAAAAATCCGATTAATTAATTTTCTACAACTTTTAACAAAAAATTTTATTGAATGTTGTTATGTGTAGACTAAAGCAATCAAAGTGAGTGATGTAACATATAGAGGGATTAGTGGAACATCATTAACAGACAAGGCAATAAATTTGAACTGCGACCAAAATGTGGGGTGCTCTAACCTTGTATTTGATCGTGTTTATGTAAGATCTGCTGTTCCTAAGATGAAGGTTTTCTCCTTCTGCCATAATGCTCATGGAAGAGCCTCACACACCAAACCAATTCTAAACTGCTTGCTCAAGTAG
Protein sequence

>MS.gene23957.t1

MILSMFKLGLSSTTTFNVLQYGAVGDGKTNDSPAFLKAWKDVCQSKSGTSRLIIPAAKTFLLRPIAFRGPCKSNYIYIELSGNIVAPKTKSEYSGSQINTWIGFSFVNGLIISGKGTIDGRGSMWWKQPCIGNPPPGTSCRPPTAITLNRCYRFQIKGYTSVNPARSHITLTSCKKGIISNIRLIAPGESPNTDGIDISASRDIQVLNSFIATGDDCIAISAGSSVIKITAITCGPGHGISIGSLGARGDTDIVEDVHVKNCTLTKTLTGVRIKTKQGGGGFARRITFENIRFVRAHNPIMIDQFYCVNQMVCKNMTKAIKVSDVTYRGISGTSLTDKAINLNCDQNVGCSNLVFDRVYVRSAVPKMKVFSFCHNAHGRASHTKPILNCLLK