Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene24282.t1 | XP_013466099.1 | 97.2 | 463 | 12 | 1 | 1 | 462 | 6 | 468 | 7.60E-241 | 842.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene24282.t1 | Q9FLD5 | 60.6 | 459 | 166 | 4 | 1 | 445 | 7 | 464 | 1.9e-147 | 523.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene24282.t1 | A0A072VFB1 | 97.2 | 463 | 12 | 1 | 1 | 462 | 6 | 468 | 5.4e-241 | 842.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene051003 | MS.gene24282 | 0.813954 | 1.97E-51 | -1.69E-46 |
| MS.gene051184 | MS.gene24282 | 0.800433 | 1.44E-48 | -1.69E-46 |
| MS.gene053295 | MS.gene24282 | 0.966934 | 1.38E-126 | -1.69E-46 |
| MS.gene053564 | MS.gene24282 | 0.81 | 1.43E-50 | -1.69E-46 |
| MS.gene053786 | MS.gene24282 | 0.803512 | 3.37E-49 | -1.69E-46 |
| MS.gene057622 | MS.gene24282 | 0.803044 | 4.21E-49 | -1.69E-46 |
| MS.gene059146 | MS.gene24282 | 0.803564 | 3.28E-49 | -1.69E-46 |
| MS.gene06011 | MS.gene24282 | 0.827575 | 1.45E-54 | -1.69E-46 |
| MS.gene060763 | MS.gene24282 | 0.800739 | 1.25E-48 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene24282.t1 | MTR_1g022250 | 97.840 | 463 | 9 | 1 | 1 | 462 | 6 | 468 | 0.0 | 937 |
| MS.gene24282.t1 | MTR_2g030420 | 88.261 | 460 | 50 | 1 | 1 | 460 | 9 | 464 | 0.0 | 832 |
| MS.gene24282.t1 | MTR_4g107370 | 62.236 | 474 | 159 | 4 | 1 | 455 | 9 | 481 | 0.0 | 596 |
| MS.gene24282.t1 | MTR_7g008040 | 63.478 | 460 | 150 | 6 | 1 | 445 | 4 | 460 | 0.0 | 588 |
| MS.gene24282.t1 | MTR_6g009520 | 58.862 | 457 | 175 | 4 | 1 | 446 | 8 | 462 | 0.0 | 545 |
| MS.gene24282.t1 | MTR_6g009540 | 56.455 | 457 | 186 | 4 | 1 | 446 | 6 | 460 | 0.0 | 541 |
| MS.gene24282.t1 | MTR_6g009720 | 57.359 | 462 | 178 | 6 | 1 | 444 | 8 | 468 | 0.0 | 536 |
| MS.gene24282.t1 | MTR_6g009553 | 57.238 | 449 | 172 | 6 | 18 | 446 | 1 | 449 | 0.0 | 518 |
| MS.gene24282.t1 | MTR_1g022245 | 56.739 | 460 | 180 | 4 | 1 | 444 | 4 | 460 | 0.0 | 517 |
| MS.gene24282.t1 | MTR_6g009770 | 55.388 | 464 | 166 | 7 | 1 | 446 | 8 | 448 | 3.28e-175 | 501 |
| MS.gene24282.t1 | MTR_6g042290 | 54.444 | 450 | 173 | 6 | 7 | 445 | 23 | 451 | 6.36e-171 | 490 |
| MS.gene24282.t1 | MTR_6g009510 | 52.125 | 447 | 149 | 7 | 4 | 445 | 20 | 406 | 3.98e-155 | 448 |
| MS.gene24282.t1 | MTR_6g009660 | 47.894 | 451 | 185 | 6 | 2 | 447 | 8 | 413 | 2.50e-140 | 411 |
| MS.gene24282.t1 | MTR_5g020980 | 41.970 | 467 | 232 | 7 | 1 | 453 | 13 | 454 | 3.43e-122 | 365 |
| MS.gene24282.t1 | MTR_4g128260 | 45.023 | 442 | 220 | 6 | 2 | 425 | 5 | 441 | 1.44e-121 | 365 |
| MS.gene24282.t1 | MTR_2g076970 | 43.902 | 451 | 216 | 9 | 7 | 448 | 9 | 431 | 2.64e-117 | 352 |
| MS.gene24282.t1 | MTR_2g076690 | 42.795 | 458 | 222 | 10 | 7 | 452 | 16 | 445 | 7.48e-113 | 340 |
| MS.gene24282.t1 | MTR_2g076710 | 41.758 | 455 | 225 | 8 | 7 | 448 | 14 | 441 | 2.38e-112 | 338 |
| MS.gene24282.t1 | MTR_2g061820 | 42.956 | 433 | 228 | 7 | 4 | 425 | 2 | 426 | 4.16e-112 | 340 |
| MS.gene24282.t1 | MTR_2g076920 | 42.888 | 457 | 217 | 11 | 7 | 452 | 15 | 438 | 9.46e-112 | 338 |
| MS.gene24282.t1 | MTR_2g076840 | 43.421 | 456 | 220 | 8 | 7 | 452 | 9 | 436 | 1.20e-110 | 343 |
| MS.gene24282.t1 | MTR_2g076840 | 51.449 | 276 | 110 | 4 | 173 | 446 | 451 | 704 | 4.95e-84 | 273 |
| MS.gene24282.t1 | MTR_2g076990 | 44.110 | 399 | 192 | 6 | 58 | 448 | 1 | 376 | 4.11e-108 | 327 |
| MS.gene24282.t1 | MTR_2g076900 | 40.299 | 469 | 239 | 9 | 7 | 461 | 9 | 450 | 6.26e-107 | 325 |
| MS.gene24282.t1 | MTR_8g076920 | 39.333 | 450 | 229 | 8 | 6 | 440 | 28 | 448 | 3.23e-105 | 322 |
| MS.gene24282.t1 | MTR_5g020990 | 38.574 | 477 | 253 | 11 | 2 | 451 | 16 | 479 | 3.91e-97 | 301 |
| MS.gene24282.t1 | MTR_3g103150 | 45.238 | 294 | 138 | 4 | 154 | 445 | 4 | 276 | 1.16e-82 | 256 |
| MS.gene24282.t1 | MTR_7g117950 | 34.596 | 396 | 204 | 11 | 56 | 445 | 59 | 405 | 2.23e-66 | 220 |
| MS.gene24282.t1 | MTR_3g092060 | 32.911 | 395 | 211 | 8 | 61 | 446 | 66 | 415 | 2.96e-63 | 213 |
| MS.gene24282.t1 | MTR_5g021020 | 37.097 | 310 | 154 | 7 | 138 | 445 | 135 | 405 | 5.29e-55 | 189 |
| MS.gene24282.t1 | MTR_8g027200 | 29.646 | 452 | 234 | 12 | 22 | 458 | 24 | 406 | 3.33e-53 | 185 |
| MS.gene24282.t1 | MTR_1g011680 | 61.983 | 121 | 46 | 0 | 324 | 444 | 13 | 133 | 2.77e-44 | 153 |
| MS.gene24282.t1 | MTR_5g021000 | 55.556 | 108 | 43 | 2 | 340 | 445 | 1 | 105 | 9.36e-33 | 122 |
| MS.gene24282.t1 | MTR_5g020720 | 36.735 | 196 | 86 | 4 | 252 | 445 | 26 | 185 | 4.02e-27 | 108 |
| MS.gene24282.t1 | MTR_1g113750 | 61.644 | 73 | 28 | 0 | 370 | 442 | 13 | 85 | 1.99e-22 | 92.8 |
| MS.gene24282.t1 | MTR_5g021010 | 48.352 | 91 | 42 | 2 | 357 | 445 | 34 | 121 | 4.15e-19 | 83.6 |
| MS.gene24282.t1 | MTR_2g101290 | 28.497 | 193 | 105 | 5 | 200 | 384 | 423 | 590 | 9.01e-14 | 73.9 |
| MS.gene24282.t1 | MTR_2g101290 | 28.497 | 193 | 105 | 5 | 200 | 384 | 423 | 590 | 1.47e-13 | 73.6 |
| MS.gene24282.t1 | MTR_2g076820 | 59.615 | 52 | 21 | 0 | 373 | 424 | 1 | 52 | 1.25e-12 | 63.5 |
| MS.gene24282.t1 | MTR_6g016600 | 27.869 | 244 | 133 | 9 | 220 | 448 | 345 | 560 | 1.15e-11 | 67.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene24282.t1 | AT5G40010 | 59.341 | 455 | 170 | 4 | 1 | 441 | 7 | 460 | 0.0 | 553 |
| MS.gene24282.t1 | AT3G28580 | 59.111 | 450 | 174 | 3 | 1 | 441 | 7 | 455 | 0.0 | 537 |
| MS.gene24282.t1 | AT3G28510 | 57.768 | 457 | 180 | 4 | 6 | 452 | 12 | 465 | 0.0 | 521 |
| MS.gene24282.t1 | AT3G28580 | 55.333 | 450 | 169 | 4 | 1 | 441 | 7 | 433 | 1.03e-171 | 491 |
| MS.gene24282.t1 | AT3G28540 | 54.054 | 481 | 197 | 6 | 1 | 462 | 7 | 482 | 1.31e-171 | 492 |
| MS.gene24282.t1 | AT3G28540 | 54.054 | 481 | 197 | 6 | 1 | 462 | 7 | 482 | 1.58e-171 | 492 |
| MS.gene24282.t1 | AT3G28600 | 50.431 | 464 | 183 | 7 | 5 | 445 | 7 | 446 | 1.15e-159 | 460 |
| MS.gene24282.t1 | AT3G28610 | 48.460 | 487 | 198 | 8 | 1 | 461 | 2 | 461 | 7.04e-159 | 458 |
| MS.gene24282.t1 | AT3G28520 | 53.898 | 449 | 186 | 6 | 9 | 444 | 1 | 441 | 4.80e-158 | 456 |
| MS.gene24282.t1 | AT3G28570 | 49.897 | 485 | 183 | 11 | 1 | 461 | 8 | 456 | 2.28e-154 | 446 |
| MS.gene24282.t1 | AT5G40000 | 49.278 | 485 | 191 | 9 | 2 | 461 | 8 | 462 | 2.49e-154 | 446 |
| MS.gene24282.t1 | AT3G28570 | 49.897 | 485 | 183 | 11 | 1 | 461 | 1 | 449 | 3.77e-154 | 445 |
| MS.gene24282.t1 | AT3G28600 | 47.629 | 464 | 157 | 7 | 5 | 445 | 7 | 407 | 1.49e-141 | 413 |
| MS.gene24282.t1 | AT4G25835 | 44.725 | 436 | 223 | 4 | 2 | 425 | 5 | 434 | 6.51e-124 | 370 |
| MS.gene24282.t1 | AT5G57480 | 41.807 | 476 | 252 | 6 | 2 | 460 | 5 | 472 | 3.13e-122 | 366 |
| MS.gene24282.t1 | AT3G50940 | 42.177 | 441 | 221 | 9 | 17 | 449 | 31 | 445 | 1.04e-115 | 347 |
| MS.gene24282.t1 | AT3G50930 | 42.350 | 451 | 228 | 7 | 13 | 450 | 73 | 504 | 8.16e-114 | 347 |
| MS.gene24282.t1 | AT4G30250 | 42.672 | 464 | 237 | 7 | 2 | 445 | 7 | 461 | 2.43e-110 | 336 |
| MS.gene24282.t1 | AT4G30250 | 42.672 | 464 | 237 | 7 | 2 | 445 | 5 | 459 | 2.93e-110 | 335 |
| MS.gene24282.t1 | AT2G18193 | 41.394 | 459 | 222 | 10 | 6 | 450 | 19 | 444 | 1.70e-106 | 325 |
| MS.gene24282.t1 | AT5G17740 | 39.121 | 478 | 250 | 13 | 1 | 462 | 13 | 465 | 6.61e-102 | 315 |
| MS.gene24282.t1 | AT5G17760 | 41.487 | 417 | 202 | 6 | 59 | 457 | 70 | 462 | 2.67e-101 | 312 |
| MS.gene24282.t1 | AT2G18190 | 39.066 | 471 | 244 | 9 | 1 | 457 | 15 | 456 | 3.83e-101 | 311 |
| MS.gene24282.t1 | AT1G43910 | 39.514 | 453 | 247 | 8 | 1 | 445 | 14 | 447 | 6.54e-96 | 297 |
| MS.gene24282.t1 | AT4G05380 | 38.377 | 456 | 253 | 9 | 1 | 448 | 15 | 450 | 1.59e-94 | 293 |
| MS.gene24282.t1 | AT5G17730 | 35.242 | 454 | 262 | 8 | 6 | 450 | 18 | 448 | 2.88e-93 | 290 |
| MS.gene24282.t1 | AT5G17750 | 37.292 | 421 | 210 | 8 | 1 | 410 | 13 | 390 | 2.23e-88 | 275 |
| MS.gene24282.t1 | AT2G46620 | 32.346 | 439 | 235 | 8 | 22 | 445 | 25 | 416 | 2.96e-73 | 239 |
| MS.gene24282.t1 | AT5G17760 | 38.577 | 267 | 150 | 1 | 59 | 311 | 70 | 336 | 2.67e-58 | 196 |
| MS.gene24282.t1 | AT3G28560 | 42.791 | 215 | 105 | 3 | 1 | 204 | 7 | 214 | 2.20e-50 | 172 |
| MS.gene24282.t1 | AT3G29800 | 31.897 | 348 | 182 | 9 | 102 | 442 | 78 | 377 | 2.37e-47 | 169 |
| MS.gene24282.t1 | AT4G05340 | 49.485 | 97 | 39 | 2 | 304 | 397 | 4 | 93 | 1.58e-20 | 86.7 |
| MS.gene24282.t1 | AT3G16290 | 27.979 | 193 | 106 | 5 | 200 | 384 | 415 | 582 | 3.00e-12 | 69.3 |
Find 80 sgRNAs with CRISPR-Local
Find 92 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCACATCTCTATTGTGTTTA+TGG | 0.043514 | 1.1:+2067870 | None:intergenic |
| ACTAGTAATGTTACACTTTC+TGG | 0.065042 | 1.1:-2067314 | MS.gene24282:CDS |
| AAAAGAGGTTATTTACTTTA+TGG | 0.186213 | 1.1:-2067614 | MS.gene24282:CDS |
| ATACTAATAGTGGATCTTAT+TGG | 0.207885 | 1.1:-2067772 | MS.gene24282:CDS |
| AACAAATTATGTGGAGAAAT+TGG | 0.217945 | 1.1:-2067228 | MS.gene24282:CDS |
| TGAATTTCATTGATGGATTA+TGG | 0.245019 | 1.1:-2067286 | MS.gene24282:CDS |
| TTGCTTCCTTGATGTTTGTT+TGG | 0.248657 | 1.1:-2068270 | MS.gene24282:CDS |
| AAACATCAAGGAAGCAATTA+TGG | 0.257321 | 1.1:+2068276 | None:intergenic |
| AAAAGAAGGTAAAGCAATTA+AGG | 0.288836 | 1.1:-2067819 | MS.gene24282:CDS |
| TTTGTGAGTATTTCTCAATT+TGG | 0.291370 | 1.1:+2068209 | None:intergenic |
| AATTTGGTTCCTAAGTTGAT+AGG | 0.297058 | 1.1:+2068225 | None:intergenic |
| TGCTTCCTTGATGTTTGTTT+GGG | 0.301724 | 1.1:-2068269 | MS.gene24282:CDS |
| TGAAACATTAGCAATGGATT+TGG | 0.307810 | 1.1:-2067717 | MS.gene24282:CDS |
| ATTGAATCACATAATTTGTT+TGG | 0.322491 | 1.1:-2067107 | MS.gene24282:CDS |
| GCATCACCAGAAGCAGTCTT+TGG | 0.340573 | 1.1:+2067020 | None:intergenic |
| GCTGCTAAGGTAATTCTCTA+TGG | 0.355914 | 1.1:+2068099 | None:intergenic |
| GGACTATTGAATTTCATTGA+TGG | 0.363024 | 1.1:-2067293 | MS.gene24282:CDS |
| TTGATGCCAAAGACTGCTTC+TGG | 0.363892 | 1.1:-2067026 | MS.gene24282:CDS |
| GCTGCCATGGCAAATTTGTT+AGG | 0.379161 | 1.1:-2067560 | MS.gene24282:CDS |
| TGAACTCATGAAATGTGATT+TGG | 0.386423 | 1.1:+2068158 | None:intergenic |
| GTAATTCTCTATGGAAGAAT+AGG | 0.394421 | 1.1:+2068108 | None:intergenic |
| CTTATTACATTTAGTAAAGC+TGG | 0.395330 | 1.1:-2067668 | MS.gene24282:CDS |
| AGGAGGAAGAGAAGGATTCA+AGG | 0.400269 | 1.1:-2067376 | MS.gene24282:CDS |
| TCTTGAATTGACTGCTGTTA+AGG | 0.402564 | 1.1:-2067522 | MS.gene24282:CDS |
| CTTTATGGTCCTCCAGGGAC+TGG | 0.407910 | 1.1:-2067599 | MS.gene24282:CDS |
| GTGTTGATGCTTTGCTGCTA+AGG | 0.408577 | 1.1:+2068086 | None:intergenic |
| GGTGAGTTCTATGCAAGAAT+TGG | 0.409467 | 1.1:-2067647 | MS.gene24282:CDS |
| GGTGACATGTTTGCTCAAAT+AGG | 0.410316 | 1.1:-2068301 | None:intergenic |
| ATGCAAGAATTGGAAGGGCT+TGG | 0.415600 | 1.1:-2067637 | MS.gene24282:CDS |
| ATTGTTGACTTTCCAGTCCC+TGG | 0.415863 | 1.1:+2067587 | None:intergenic |
| TGGTCAAAGGAGGAAGAAAA+AGG | 0.417013 | 1.1:-2067411 | MS.gene24282:CDS |
| AGGAAACTTTATACTAATAG+TGG | 0.434469 | 1.1:-2067782 | MS.gene24282:CDS |
| AATTCAATGGAGTTAAACTT+TGG | 0.438355 | 1.1:-2067979 | MS.gene24282:CDS |
| ATTGGAAGGGCTTGGAAAAG+AGG | 0.439570 | 1.1:-2067629 | MS.gene24282:CDS |
| TATTTACTTTATGGTCCTCC+AGG | 0.463635 | 1.1:-2067605 | MS.gene24282:CDS |
| AAAGGAGAGAAAGGAAGAGG+AGG | 0.467105 | 1.1:-2067393 | MS.gene24282:CDS |
| AAAGGAAGAGGAGGAAGAGA+AGG | 0.471794 | 1.1:-2067384 | MS.gene24282:CDS |
| GTCAACAATGATTGCTGCCA+TGG | 0.475118 | 1.1:-2067573 | MS.gene24282:CDS |
| AACATTTGAAACATTAGCAA+TGG | 0.477196 | 1.1:-2067723 | MS.gene24282:CDS |
| ATTTGGTTCCTAAGTTGATA+GGG | 0.478933 | 1.1:+2068226 | None:intergenic |
| CTTAATTATGTACTAAAAGA+AGG | 0.479074 | 1.1:-2067833 | MS.gene24282:CDS |
| GATCCTGCATTGGTTAGAAA+AGG | 0.480913 | 1.1:-2067206 | MS.gene24282:CDS |
| CATATAGAGTTGTCATATTG+TGG | 0.481944 | 1.1:-2067170 | MS.gene24282:CDS |
| CAATGGAGTTAAACTTTGGT+GGG | 0.482273 | 1.1:-2067975 | MS.gene24282:CDS |
| TGAAATGTGATTTGGATGTA+AGG | 0.487481 | 1.1:+2068166 | None:intergenic |
| AACAATATGTGAATTGTTGA+AGG | 0.490020 | 1.1:-2067084 | MS.gene24282:CDS |
| AAATGCTCAGCAACATCAGC+TGG | 0.493061 | 1.1:+2067047 | None:intergenic |
| TCAATGGAGTTAAACTTTGG+TGG | 0.498351 | 1.1:-2067976 | MS.gene24282:CDS |
| GATTGCTCACTTGATTTGAC+TGG | 0.504911 | 1.1:-2067431 | MS.gene24282:CDS |
| GTTGTCATATTGTGGATTTG+AGG | 0.509085 | 1.1:-2067162 | MS.gene24282:CDS |
| GAAGTAGGTGATGAATTCAA+TGG | 0.510659 | 1.1:-2067992 | MS.gene24282:CDS |
| ATTTGAGGCATTCAAGATGT+TGG | 0.512524 | 1.1:-2067147 | MS.gene24282:CDS |
| TTTGATTCAAGCACTTGAGT+TGG | 0.513367 | 1.1:-2066979 | MS.gene24282:CDS |
| GGAGGAAGAGAAGGATTCAA+GGG | 0.514417 | 1.1:-2067375 | MS.gene24282:CDS |
| GCTAATGTTTCAAATGTTGA+AGG | 0.514554 | 1.1:+2067728 | None:intergenic |
| AGTTGGCAAAAGAAGAAGCT+AGG | 0.520457 | 1.1:-2066962 | MS.gene24282:CDS |
| TCAAGCACTTGTTCTTAGCA+TGG | 0.533913 | 1.1:-2068026 | MS.gene24282:CDS |
| ATCACATTTCATGAGTTCAC+TGG | 0.535244 | 1.1:-2068154 | MS.gene24282:CDS |
| CACTTGATTTGACTGGTCAA+AGG | 0.548667 | 1.1:-2067424 | MS.gene24282:CDS |
| ATTGGTTAGAAAAGGTAGAA+TGG | 0.558531 | 1.1:-2067198 | MS.gene24282:CDS |
| AGAAGCTAGGGTGAAATCAG+TGG | 0.560095 | 1.1:-2066949 | MS.gene24282:CDS |
| ATGGATGATCATGAAGAAGT+AGG | 0.568240 | 1.1:-2068007 | MS.gene24282:CDS |
| GTTAAACTTTGGTGGGCTAG+TGG | 0.573542 | 1.1:-2067968 | MS.gene24282:CDS |
| AATAGCCCAAACAAACATCA+AGG | 0.574656 | 1.1:+2068264 | None:intergenic |
| GGAATTATGGAGGAAAGAGA+TGG | 0.581168 | 1.1:-2067341 | MS.gene24282:CDS |
| ATTTACTTTATGGTCCTCCA+GGG | 0.585163 | 1.1:-2067604 | MS.gene24282:CDS |
| GGAGAAATTGGATCCTGCAT+TGG | 0.595123 | 1.1:-2067216 | MS.gene24282:CDS |
| AGTTCTATGCAAGAATTGGA+AGG | 0.596527 | 1.1:-2067643 | MS.gene24282:CDS |
| ACACAAGCAAAGAGACTCAA+AGG | 0.600263 | 1.1:-2068067 | MS.gene24282:CDS |
| TTGATTTGACTGGTCAAAGG+AGG | 0.608711 | 1.1:-2067421 | MS.gene24282:CDS |
| GAAATGTGATTTGGATGTAA+GGG | 0.622484 | 1.1:+2068167 | None:intergenic |
| GTTGACTTTCCAGTCCCTGG+AGG | 0.623801 | 1.1:+2067590 | None:intergenic |
| GTTGGCAAAAGAAGAAGCTA+GGG | 0.631329 | 1.1:-2066961 | MS.gene24282:CDS |
| GTTCTATGCAAGAATTGGAA+GGG | 0.632683 | 1.1:-2067642 | MS.gene24282:CDS |
| TTAAGGTGAGAAATAGACAA+AGG | 0.654965 | 1.1:-2067802 | MS.gene24282:CDS |
| ATAGCCTAACAAATTTGCCA+TGG | 0.654970 | 1.1:+2067556 | None:intergenic |
| GGATTATGGTCTGCATGTGG+AGG | 0.679090 | 1.1:-2067272 | MS.gene24282:CDS |
| AAGGATGTTCAAACACAACA+TGG | 0.681122 | 1.1:+2067747 | None:intergenic |
| TTAAGGATAACACTGAGTTG+AGG | 0.695176 | 1.1:-2067505 | MS.gene24282:CDS |
| GATGGATTATGGTCTGCATG+TGG | 0.714258 | 1.1:-2067275 | MS.gene24282:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAAGAGGTTATTTACTTTA+TGG | - | chr1.1:2067610-2067629 | MS.gene24282:CDS | 20.0% |
| !! | CTTAATTATGTACTAAAAGA+AGG | - | chr1.1:2067391-2067410 | MS.gene24282:CDS | 20.0% |
| !!! | AAGTGAAAATGAATTTTGTT+TGG | + | chr1.1:2067293-2067312 | None:intergenic | 20.0% |
| !!! | ATTGAATCACATAATTTGTT+TGG | - | chr1.1:2068117-2068136 | MS.gene24282:CDS | 20.0% |
| ! | AAAAGAAGGTAAAGCAATTA+AGG | - | chr1.1:2067405-2067424 | MS.gene24282:CDS | 25.0% |
| ! | AACAAATTATGTGGAGAAAT+TGG | - | chr1.1:2067996-2068015 | MS.gene24282:CDS | 25.0% |
| ! | AACAATATGTGAATTGTTGA+AGG | - | chr1.1:2068140-2068159 | MS.gene24282:CDS | 25.0% |
| ! | AATTCAATGGAGTTAAACTT+TGG | - | chr1.1:2067245-2067264 | MS.gene24282:CDS | 25.0% |
| ! | AGGAAACTTTATACTAATAG+TGG | - | chr1.1:2067442-2067461 | MS.gene24282:CDS | 25.0% |
| ! | ATCACAACATAGATGAAAAA+AGG | - | chr1.1:2067314-2067333 | MS.gene24282:CDS | 25.0% |
| ! | CTTATTACATTTAGTAAAGC+TGG | - | chr1.1:2067556-2067575 | MS.gene24282:CDS | 25.0% |
| ! | TGAGAAATACTCACAAAAAT+TGG | - | chr1.1:2067021-2067040 | MS.gene24282:CDS | 25.0% |
| ! | TTTGTGAGTATTTCTCAATT+TGG | + | chr1.1:2067018-2067037 | None:intergenic | 25.0% |
| !! | AACATTTGAAACATTAGCAA+TGG | - | chr1.1:2067501-2067520 | MS.gene24282:CDS | 25.0% |
| !! | AGTTTTCACAACAAATTATG+TGG | - | chr1.1:2067987-2068006 | MS.gene24282:CDS | 25.0% |
| !!! | AATTTTTTCCCTATCAACTT+AGG | - | chr1.1:2066990-2067009 | MS.gene24282:CDS | 25.0% |
| !!! | ATACTAATAGTGGATCTTAT+TGG | - | chr1.1:2067452-2067471 | MS.gene24282:CDS | 25.0% |
| !!! | TGAATTTCATTGATGGATTA+TGG | - | chr1.1:2067938-2067957 | MS.gene24282:CDS | 25.0% |
| AAACATCAAGGAAGCAATTA+TGG | + | chr1.1:2066951-2066970 | None:intergenic | 30.0% | |
| ACTAGTAATGTTACACTTTC+TGG | - | chr1.1:2067910-2067929 | MS.gene24282:CDS | 30.0% | |
| ATTGGTTAGAAAAGGTAGAA+TGG | - | chr1.1:2068026-2068045 | MS.gene24282:CDS | 30.0% | |
| CATATAGAGTTGTCATATTG+TGG | - | chr1.1:2068054-2068073 | MS.gene24282:CDS | 30.0% | |
| GCTAATGTTTCAAATGTTGA+AGG | + | chr1.1:2067499-2067518 | None:intergenic | 30.0% | |
| GGACTATTGAATTTCATTGA+TGG | - | chr1.1:2067931-2067950 | MS.gene24282:CDS | 30.0% | |
| GTAATTCTCTATGGAAGAAT+AGG | + | chr1.1:2067119-2067138 | None:intergenic | 30.0% | |
| TCACATCTCTATTGTGTTTA+TGG | + | chr1.1:2067357-2067376 | None:intergenic | 30.0% | |
| TGAACTCATGAAATGTGATT+TGG | + | chr1.1:2067069-2067088 | None:intergenic | 30.0% | |
| TTAAGGTGAGAAATAGACAA+AGG | - | chr1.1:2067422-2067441 | MS.gene24282:CDS | 30.0% | |
| ! | CACAATAGAGATGTGATTTT+AGG | - | chr1.1:2067361-2067380 | MS.gene24282:CDS | 30.0% |
| ! | GAAATGTGATTTGGATGTAA+GGG | + | chr1.1:2067060-2067079 | None:intergenic | 30.0% |
| ! | TGAAACATTAGCAATGGATT+TGG | - | chr1.1:2067507-2067526 | MS.gene24282:CDS | 30.0% |
| ! | TGAAATGTGATTTGGATGTA+AGG | + | chr1.1:2067061-2067080 | None:intergenic | 30.0% |
| !! | AATTTGGTTCCTAAGTTGAT+AGG | + | chr1.1:2067002-2067021 | None:intergenic | 30.0% |
| !! | ATTTGGTTCCTAAGTTGATA+GGG | + | chr1.1:2067001-2067020 | None:intergenic | 30.0% |
| AAGGATGTTCAAACACAACA+TGG | + | chr1.1:2067480-2067499 | None:intergenic | 35.0% | |
| AAGGATTCAAGGGAGAAAAA+AGG | - | chr1.1:2067859-2067878 | MS.gene24282:CDS | 35.0% | |
| AATAGCCCAAACAAACATCA+AGG | + | chr1.1:2066963-2066982 | None:intergenic | 35.0% | |
| AGTTCTATGCAAGAATTGGA+AGG | - | chr1.1:2067581-2067600 | MS.gene24282:CDS | 35.0% | |
| ATAGCCTAACAAATTTGCCA+TGG | + | chr1.1:2067671-2067690 | None:intergenic | 35.0% | |
| ATCACATTTCATGAGTTCAC+TGG | - | chr1.1:2067070-2067089 | MS.gene24282:CDS | 35.0% | |
| ATGGATGATCATGAAGAAGT+AGG | - | chr1.1:2067217-2067236 | MS.gene24282:CDS | 35.0% | |
| ATTTACTTTATGGTCCTCCA+GGG | - | chr1.1:2067620-2067639 | MS.gene24282:CDS | 35.0% | |
| CAATGGAGTTAAACTTTGGT+GGG | - | chr1.1:2067249-2067268 | MS.gene24282:CDS | 35.0% | |
| GAAAAAAGGTGGAATTATGG+AGG | - | chr1.1:2067873-2067892 | MS.gene24282:CDS | 35.0% | |
| GAAGTAGGTGATGAATTCAA+TGG | - | chr1.1:2067232-2067251 | MS.gene24282:CDS | 35.0% | |
| GGAGAAAAAAGGTGGAATTA+TGG | - | chr1.1:2067870-2067889 | MS.gene24282:CDS | 35.0% | |
| GTTCTATGCAAGAATTGGAA+GGG | - | chr1.1:2067582-2067601 | MS.gene24282:CDS | 35.0% | |
| GTTGTCATATTGTGGATTTG+AGG | - | chr1.1:2068062-2068081 | MS.gene24282:CDS | 35.0% | |
| TATTTACTTTATGGTCCTCC+AGG | - | chr1.1:2067619-2067638 | MS.gene24282:CDS | 35.0% | |
| TCAATGGAGTTAAACTTTGG+TGG | - | chr1.1:2067248-2067267 | MS.gene24282:CDS | 35.0% | |
| TCTTGAATTGACTGCTGTTA+AGG | - | chr1.1:2067702-2067721 | MS.gene24282:CDS | 35.0% | |
| TGCTTCCTTGATGTTTGTTT+GGG | - | chr1.1:2066955-2066974 | MS.gene24282:CDS | 35.0% | |
| TTAAGGATAACACTGAGTTG+AGG | - | chr1.1:2067719-2067738 | MS.gene24282:CDS | 35.0% | |
| TTGCTTCCTTGATGTTTGTT+TGG | - | chr1.1:2066954-2066973 | MS.gene24282:CDS | 35.0% | |
| TTTGATTCAAGCACTTGAGT+TGG | - | chr1.1:2068245-2068264 | MS.gene24282:CDS | 35.0% | |
| ! | ATTTGAGGCATTCAAGATGT+TGG | - | chr1.1:2068077-2068096 | MS.gene24282:CDS | 35.0% |
| ACACAAGCAAAGAGACTCAA+AGG | - | chr1.1:2067157-2067176 | MS.gene24282:CDS | 40.0% | |
| AGTTGGCAAAAGAAGAAGCT+AGG | - | chr1.1:2068262-2068281 | MS.gene24282:CDS | 40.0% | |
| GAAAAAGGAGAGAAAGGAAG+AGG | - | chr1.1:2067828-2067847 | MS.gene24282:CDS | 40.0% | |
| GAGGAAGAAAAAGGAGAGAA+AGG | - | chr1.1:2067822-2067841 | MS.gene24282:CDS | 40.0% | |
| GATTCAAGGGAGAAAAAAGG+TGG | - | chr1.1:2067862-2067881 | MS.gene24282:CDS | 40.0% | |
| GCTGCTAAGGTAATTCTCTA+TGG | + | chr1.1:2067128-2067147 | None:intergenic | 40.0% | |
| GGAATTATGGAGGAAAGAGA+TGG | - | chr1.1:2067883-2067902 | MS.gene24282:CDS | 40.0% | |
| GGTGAGTTCTATGCAAGAAT+TGG | - | chr1.1:2067577-2067596 | MS.gene24282:CDS | 40.0% | |
| GTTGGCAAAAGAAGAAGCTA+GGG | - | chr1.1:2068263-2068282 | MS.gene24282:CDS | 40.0% | |
| TCAAGCACTTGTTCTTAGCA+TGG | - | chr1.1:2067198-2067217 | MS.gene24282:CDS | 40.0% | |
| TGGTCAAAGGAGGAAGAAAA+AGG | - | chr1.1:2067813-2067832 | MS.gene24282:CDS | 40.0% | |
| ! | CACTTGATTTGACTGGTCAA+AGG | - | chr1.1:2067800-2067819 | MS.gene24282:CDS | 40.0% |
| ! | CTACCTTTTCTAACCAATGC+AGG | + | chr1.1:2068024-2068043 | None:intergenic | 40.0% |
| ! | GATTGCTCACTTGATTTGAC+TGG | - | chr1.1:2067793-2067812 | MS.gene24282:CDS | 40.0% |
| ! | TTGATTTGACTGGTCAAAGG+AGG | - | chr1.1:2067803-2067822 | MS.gene24282:CDS | 40.0% |
| !! | GATCCTGCATTGGTTAGAAA+AGG | - | chr1.1:2068018-2068037 | MS.gene24282:CDS | 40.0% |
| AAAGGAAGAGGAGGAAGAGA+AGG | - | chr1.1:2067840-2067859 | MS.gene24282:CDS | 45.0% | |
| AAAGGAGAGAAAGGAAGAGG+AGG | - | chr1.1:2067831-2067850 | MS.gene24282:CDS | 45.0% | |
| AAATGCTCAGCAACATCAGC+TGG | + | chr1.1:2068180-2068199 | None:intergenic | 45.0% | |
| AGAAGCTAGGGTGAAATCAG+TGG | - | chr1.1:2068275-2068294 | MS.gene24282:CDS | 45.0% | |
| AGGAGGAAGAGAAGGATTCA+AGG | - | chr1.1:2067848-2067867 | MS.gene24282:CDS | 45.0% | |
| ATGCAAGAATTGGAAGGGCT+TGG | - | chr1.1:2067587-2067606 | MS.gene24282:CDS | 45.0% | |
| ATTGGAAGGGCTTGGAAAAG+AGG | - | chr1.1:2067595-2067614 | MS.gene24282:CDS | 45.0% | |
| GGAGAAATTGGATCCTGCAT+TGG | - | chr1.1:2068008-2068027 | MS.gene24282:CDS | 45.0% | |
| GGAGGAAGAGAAGGATTCAA+GGG | - | chr1.1:2067849-2067868 | MS.gene24282:CDS | 45.0% | |
| GTCAACAATGATTGCTGCCA+TGG | - | chr1.1:2067651-2067670 | MS.gene24282:CDS | 45.0% | |
| TTGATGCCAAAGACTGCTTC+TGG | - | chr1.1:2068198-2068217 | MS.gene24282:CDS | 45.0% | |
| ! | GCTGCCATGGCAAATTTGTT+AGG | - | chr1.1:2067664-2067683 | MS.gene24282:CDS | 45.0% |
| ! | GTGTTGATGCTTTGCTGCTA+AGG | + | chr1.1:2067141-2067160 | None:intergenic | 45.0% |
| !! | ATTGTTGACTTTCCAGTCCC+TGG | + | chr1.1:2067640-2067659 | None:intergenic | 45.0% |
| !! | GATGGATTATGGTCTGCATG+TGG | - | chr1.1:2067949-2067968 | MS.gene24282:CDS | 45.0% |
| !! | GTTAAACTTTGGTGGGCTAG+TGG | - | chr1.1:2067256-2067275 | MS.gene24282:CDS | 45.0% |
| GCATCACCAGAAGCAGTCTT+TGG | + | chr1.1:2068207-2068226 | None:intergenic | 50.0% | |
| GGATTATGGTCTGCATGTGG+AGG | - | chr1.1:2067952-2067971 | MS.gene24282:CDS | 50.0% | |
| CTTTATGGTCCTCCAGGGAC+TGG | - | chr1.1:2067625-2067644 | MS.gene24282:CDS | 55.0% | |
| ! | GTTGACTTTCCAGTCCCTGG+AGG | + | chr1.1:2067637-2067656 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.1 | gene | 2066929 | 2068317 | 2066929 | ID=MS.gene24282 |
| chr1.1 | mRNA | 2066929 | 2068317 | 2066929 | ID=MS.gene24282.t1;Parent=MS.gene24282 |
| chr1.1 | exon | 2066929 | 2068317 | 2066929 | ID=MS.gene24282.t1.exon1;Parent=MS.gene24282.t1 |
| chr1.1 | CDS | 2066929 | 2068317 | 2066929 | ID=cds.MS.gene24282.t1;Parent=MS.gene24282.t1 |
| Gene Sequence |
| Protein sequence |
