Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene053295.t1 | XP_013466099.1 | 97.4 | 463 | 11 | 1 | 1 | 462 | 6 | 468 | 2.60E-241 | 844.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene053295.t1 | Q9FLD5 | 60.6 | 459 | 166 | 4 | 1 | 445 | 7 | 464 | 1.9e-147 | 523.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene053295.t1 | A0A072VFB1 | 97.4 | 463 | 11 | 1 | 1 | 462 | 6 | 468 | 1.9e-241 | 844.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene051003 | MS.gene053295 | 0.804827 | 1.79E-49 | -1.69E-46 |
| MS.gene053295 | MS.gene053564 | 0.800997 | 1.11E-48 | -1.69E-46 |
| MS.gene053295 | MS.gene053786 | 0.801834 | 7.47E-49 | -1.69E-46 |
| MS.gene053295 | MS.gene057622 | 0.812057 | 5.13E-51 | -1.69E-46 |
| MS.gene053295 | MS.gene059146 | 0.800019 | 1.75E-48 | -1.69E-46 |
| MS.gene053295 | MS.gene06011 | 0.8216 | 3.70E-53 | -1.69E-46 |
| MS.gene053295 | MS.gene060763 | 0.802626 | 5.13E-49 | -1.69E-46 |
| MS.gene053295 | MS.gene06131 | 0.802108 | 6.56E-49 | -1.69E-46 |
| MS.gene053295 | MS.gene067608 | 0.808064 | 3.72E-50 | -1.69E-46 |
| MS.gene053295 | MS.gene067857 | 0.816091 | 6.61E-52 | -1.69E-46 |
| MS.gene053295 | MS.gene08582 | 0.80657 | 7.71E-50 | -1.69E-46 |
| MS.gene053295 | MS.gene08793 | 0.803525 | 3.34E-49 | -1.69E-46 |
| MS.gene053295 | MS.gene08948 | 0.801282 | 9.69E-49 | -1.69E-46 |
| MS.gene053295 | MS.gene23464 | 0.807781 | 4.27E-50 | -1.69E-46 |
| MS.gene053295 | MS.gene24282 | 0.966934 | 1.38E-126 | -1.69E-46 |
| MS.gene053295 | MS.gene24285 | 0.801307 | 9.58E-49 | -1.69E-46 |
| MS.gene053295 | MS.gene26961 | 0.994562 | 2.81E-208 | -1.69E-46 |
| MS.gene053295 | MS.gene27315 | 0.989415 | 5.08E-178 | -1.69E-46 |
| MS.gene053295 | MS.gene29794 | 0.829467 | 5.07E-55 | -1.69E-46 |
| MS.gene053295 | MS.gene31856 | 0.806054 | 9.91E-50 | -1.69E-46 |
| MS.gene053295 | MS.gene40608 | 0.801405 | 9.14E-49 | -1.69E-46 |
| MS.gene053295 | MS.gene40991 | 0.803525 | 3.34E-49 | -1.69E-46 |
| MS.gene053295 | MS.gene41775 | 0.81732 | 3.51E-52 | -1.69E-46 |
| MS.gene053295 | MS.gene42295 | 0.807274 | 5.48E-50 | -1.69E-46 |
| MS.gene053295 | MS.gene45374 | 0.832113 | 1.14E-55 | -1.69E-46 |
| MS.gene053295 | MS.gene46408 | 0.804181 | 2.44E-49 | -1.69E-46 |
| MS.gene053295 | MS.gene47311 | 0.817371 | 3.41E-52 | -1.69E-46 |
| MS.gene053295 | MS.gene49627 | 0.817081 | 3.97E-52 | -1.69E-46 |
| MS.gene053295 | MS.gene49733 | 0.827903 | 1.21E-54 | -1.69E-46 |
| MS.gene053295 | MS.gene49816 | 0.826983 | 2.01E-54 | -1.69E-46 |
| MS.gene053295 | MS.gene55094 | 0.803274 | 3.77E-49 | -1.69E-46 |
| MS.gene053295 | MS.gene57783 | 0.804738 | 1.87E-49 | -1.69E-46 |
| MS.gene053295 | MS.gene57980 | 0.803811 | 2.92E-49 | -1.69E-46 |
| MS.gene053295 | MS.gene62676 | 0.810466 | 1.14E-50 | -1.69E-46 |
| MS.gene053295 | MS.gene62677 | 0.824458 | 7.98E-54 | -1.69E-46 |
| MS.gene053295 | MS.gene63430 | 0.801097 | 1.06E-48 | -1.69E-46 |
| MS.gene053295 | MS.gene65295 | 0.814094 | 1.84E-51 | -1.69E-46 |
| MS.gene053295 | MS.gene65518 | 0.821607 | 3.69E-53 | -1.69E-46 |
| MS.gene053295 | MS.gene67485 | 0.804958 | 1.68E-49 | -1.69E-46 |
| MS.gene053295 | MS.gene70154 | 0.8235 | 1.34E-53 | -1.69E-46 |
| MS.gene053295 | MS.gene70314 | 0.815274 | 1.01E-51 | -1.69E-46 |
| MS.gene053295 | MS.gene72748 | 0.831193 | 1.92E-55 | -1.69E-46 |
| MS.gene053295 | MS.gene72950 | -0.803782 | 2.96E-49 | -1.69E-46 |
| MS.gene053295 | MS.gene77361 | 0.82172 | 3.47E-53 | -1.69E-46 |
| MS.gene053295 | MS.gene78773 | 0.812423 | 4.27E-51 | -1.69E-46 |
| MS.gene053295 | MS.gene86455 | 0.800786 | 1.22E-48 | -1.69E-46 |
| MS.gene053295 | MS.gene86919 | 0.809436 | 1.89E-50 | -1.69E-46 |
| MS.gene053295 | MS.gene89712 | 0.817457 | 3.27E-52 | -1.69E-46 |
| MS.gene053295 | MS.gene93983 | 0.803421 | 3.51E-49 | -1.69E-46 |
| MS.gene053295 | MS.gene96690 | 0.814082 | 1.85E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene053295.t1 | MTR_1g022250 | 98.056 | 463 | 8 | 1 | 1 | 462 | 6 | 468 | 0.0 | 939 |
| MS.gene053295.t1 | MTR_2g030420 | 88.043 | 460 | 51 | 1 | 1 | 460 | 9 | 464 | 0.0 | 831 |
| MS.gene053295.t1 | MTR_4g107370 | 62.236 | 474 | 159 | 4 | 1 | 455 | 9 | 481 | 0.0 | 596 |
| MS.gene053295.t1 | MTR_7g008040 | 63.478 | 460 | 150 | 6 | 1 | 445 | 4 | 460 | 0.0 | 588 |
| MS.gene053295.t1 | MTR_6g009520 | 58.862 | 457 | 175 | 4 | 1 | 446 | 8 | 462 | 0.0 | 544 |
| MS.gene053295.t1 | MTR_6g009540 | 56.455 | 457 | 186 | 4 | 1 | 446 | 6 | 460 | 0.0 | 541 |
| MS.gene053295.t1 | MTR_6g009720 | 57.359 | 462 | 178 | 6 | 1 | 444 | 8 | 468 | 0.0 | 536 |
| MS.gene053295.t1 | MTR_6g009553 | 57.238 | 449 | 172 | 6 | 18 | 446 | 1 | 449 | 0.0 | 518 |
| MS.gene053295.t1 | MTR_1g022245 | 56.522 | 460 | 181 | 4 | 1 | 444 | 4 | 460 | 0.0 | 516 |
| MS.gene053295.t1 | MTR_6g009770 | 55.388 | 464 | 166 | 7 | 1 | 446 | 8 | 448 | 4.50e-175 | 500 |
| MS.gene053295.t1 | MTR_6g042290 | 54.444 | 450 | 173 | 6 | 7 | 445 | 23 | 451 | 9.31e-171 | 489 |
| MS.gene053295.t1 | MTR_6g009510 | 52.125 | 447 | 149 | 7 | 4 | 445 | 20 | 406 | 5.58e-155 | 447 |
| MS.gene053295.t1 | MTR_6g009660 | 47.894 | 451 | 185 | 6 | 2 | 447 | 8 | 413 | 3.70e-140 | 410 |
| MS.gene053295.t1 | MTR_5g020980 | 42.184 | 467 | 231 | 7 | 1 | 453 | 13 | 454 | 9.76e-123 | 367 |
| MS.gene053295.t1 | MTR_4g128260 | 44.796 | 442 | 221 | 6 | 2 | 425 | 5 | 441 | 3.70e-121 | 363 |
| MS.gene053295.t1 | MTR_2g076970 | 44.124 | 451 | 215 | 9 | 7 | 448 | 9 | 431 | 9.25e-118 | 353 |
| MS.gene053295.t1 | MTR_2g076690 | 43.013 | 458 | 221 | 10 | 7 | 452 | 16 | 445 | 2.06e-113 | 342 |
| MS.gene053295.t1 | MTR_2g076710 | 41.978 | 455 | 224 | 8 | 7 | 448 | 14 | 441 | 6.30e-113 | 340 |
| MS.gene053295.t1 | MTR_2g076920 | 43.107 | 457 | 216 | 11 | 7 | 452 | 15 | 438 | 3.01e-112 | 339 |
| MS.gene053295.t1 | MTR_2g061820 | 42.956 | 433 | 228 | 7 | 4 | 425 | 2 | 426 | 5.81e-112 | 340 |
| MS.gene053295.t1 | MTR_2g076840 | 43.640 | 456 | 219 | 8 | 7 | 452 | 9 | 436 | 3.90e-111 | 344 |
| MS.gene053295.t1 | MTR_2g076840 | 51.449 | 276 | 110 | 4 | 173 | 446 | 451 | 704 | 5.05e-84 | 273 |
| MS.gene053295.t1 | MTR_2g076990 | 44.361 | 399 | 191 | 6 | 58 | 448 | 1 | 376 | 1.33e-108 | 328 |
| MS.gene053295.t1 | MTR_2g076900 | 40.512 | 469 | 238 | 9 | 7 | 461 | 9 | 450 | 1.93e-107 | 326 |
| MS.gene053295.t1 | MTR_8g076920 | 39.556 | 450 | 228 | 8 | 6 | 440 | 28 | 448 | 9.98e-106 | 323 |
| MS.gene053295.t1 | MTR_5g020990 | 38.784 | 477 | 252 | 11 | 2 | 451 | 16 | 479 | 1.17e-97 | 302 |
| MS.gene053295.t1 | MTR_3g103150 | 45.238 | 294 | 138 | 4 | 154 | 445 | 4 | 276 | 1.44e-82 | 256 |
| MS.gene053295.t1 | MTR_7g117950 | 34.343 | 396 | 205 | 11 | 56 | 445 | 59 | 405 | 5.70e-66 | 219 |
| MS.gene053295.t1 | MTR_3g092060 | 32.658 | 395 | 212 | 8 | 61 | 446 | 66 | 415 | 5.51e-63 | 212 |
| MS.gene053295.t1 | MTR_5g021020 | 37.097 | 310 | 154 | 7 | 138 | 445 | 135 | 405 | 4.92e-55 | 189 |
| MS.gene053295.t1 | MTR_8g027200 | 29.425 | 452 | 235 | 12 | 22 | 458 | 24 | 406 | 6.54e-53 | 184 |
| MS.gene053295.t1 | MTR_1g011680 | 61.983 | 121 | 46 | 0 | 324 | 444 | 13 | 133 | 2.77e-44 | 153 |
| MS.gene053295.t1 | MTR_5g021000 | 55.556 | 108 | 43 | 2 | 340 | 445 | 1 | 105 | 9.36e-33 | 122 |
| MS.gene053295.t1 | MTR_5g020720 | 36.735 | 196 | 86 | 4 | 252 | 445 | 26 | 185 | 4.35e-27 | 107 |
| MS.gene053295.t1 | MTR_1g113750 | 61.644 | 73 | 28 | 0 | 370 | 442 | 13 | 85 | 2.05e-22 | 92.8 |
| MS.gene053295.t1 | MTR_5g021010 | 48.352 | 91 | 42 | 2 | 357 | 445 | 34 | 121 | 3.83e-19 | 83.6 |
| MS.gene053295.t1 | MTR_2g101290 | 28.497 | 193 | 105 | 5 | 200 | 384 | 423 | 590 | 8.78e-14 | 74.3 |
| MS.gene053295.t1 | MTR_2g101290 | 28.497 | 193 | 105 | 5 | 200 | 384 | 423 | 590 | 1.30e-13 | 73.6 |
| MS.gene053295.t1 | MTR_2g076820 | 59.615 | 52 | 21 | 0 | 373 | 424 | 1 | 52 | 1.25e-12 | 63.5 |
| MS.gene053295.t1 | MTR_6g016600 | 27.869 | 244 | 133 | 9 | 220 | 448 | 345 | 560 | 1.25e-11 | 67.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene053295.t1 | AT5G40010 | 59.341 | 455 | 170 | 4 | 1 | 441 | 7 | 460 | 0.0 | 553 |
| MS.gene053295.t1 | AT3G28580 | 59.111 | 450 | 174 | 3 | 1 | 441 | 7 | 455 | 0.0 | 536 |
| MS.gene053295.t1 | AT3G28510 | 57.456 | 456 | 183 | 2 | 6 | 452 | 12 | 465 | 0.0 | 519 |
| MS.gene053295.t1 | AT3G28580 | 55.333 | 450 | 169 | 4 | 1 | 441 | 7 | 433 | 1.37e-171 | 491 |
| MS.gene053295.t1 | AT3G28540 | 53.846 | 481 | 198 | 6 | 1 | 462 | 7 | 482 | 3.12e-171 | 491 |
| MS.gene053295.t1 | AT3G28540 | 53.846 | 481 | 198 | 6 | 1 | 462 | 7 | 482 | 4.64e-171 | 491 |
| MS.gene053295.t1 | AT3G28600 | 50.431 | 464 | 183 | 7 | 5 | 445 | 7 | 446 | 1.79e-159 | 460 |
| MS.gene053295.t1 | AT3G28610 | 48.460 | 487 | 198 | 8 | 1 | 461 | 2 | 461 | 9.87e-159 | 458 |
| MS.gene053295.t1 | AT3G28520 | 53.675 | 449 | 187 | 6 | 9 | 444 | 1 | 441 | 1.38e-157 | 455 |
| MS.gene053295.t1 | AT3G28570 | 49.897 | 485 | 183 | 11 | 1 | 461 | 8 | 456 | 4.19e-154 | 446 |
| MS.gene053295.t1 | AT5G40000 | 49.072 | 485 | 192 | 9 | 2 | 461 | 8 | 462 | 5.50e-154 | 446 |
| MS.gene053295.t1 | AT3G28570 | 49.897 | 485 | 183 | 11 | 1 | 461 | 1 | 449 | 6.57e-154 | 445 |
| MS.gene053295.t1 | AT3G28600 | 47.629 | 464 | 157 | 7 | 5 | 445 | 7 | 407 | 1.88e-141 | 412 |
| MS.gene053295.t1 | AT4G25835 | 44.495 | 436 | 224 | 4 | 2 | 425 | 5 | 434 | 1.58e-123 | 369 |
| MS.gene053295.t1 | AT5G57480 | 41.597 | 476 | 253 | 6 | 2 | 460 | 5 | 472 | 9.94e-122 | 365 |
| MS.gene053295.t1 | AT3G50940 | 42.404 | 441 | 220 | 9 | 17 | 449 | 31 | 445 | 2.57e-116 | 349 |
| MS.gene053295.t1 | AT3G50930 | 42.572 | 451 | 227 | 7 | 13 | 450 | 73 | 504 | 2.87e-114 | 348 |
| MS.gene053295.t1 | AT4G30250 | 42.457 | 464 | 238 | 7 | 2 | 445 | 5 | 459 | 6.17e-110 | 335 |
| MS.gene053295.t1 | AT4G30250 | 42.457 | 464 | 238 | 7 | 2 | 445 | 7 | 461 | 7.06e-110 | 335 |
| MS.gene053295.t1 | AT2G18193 | 41.176 | 459 | 223 | 10 | 6 | 450 | 19 | 444 | 4.20e-106 | 324 |
| MS.gene053295.t1 | AT5G17740 | 39.331 | 478 | 249 | 13 | 1 | 462 | 13 | 465 | 1.86e-102 | 316 |
| MS.gene053295.t1 | AT5G17760 | 41.727 | 417 | 201 | 6 | 59 | 457 | 70 | 462 | 7.92e-102 | 313 |
| MS.gene053295.t1 | AT2G18190 | 39.066 | 471 | 244 | 9 | 1 | 457 | 15 | 456 | 4.13e-101 | 311 |
| MS.gene053295.t1 | AT1G43910 | 39.735 | 453 | 246 | 8 | 1 | 445 | 14 | 447 | 1.80e-96 | 299 |
| MS.gene053295.t1 | AT4G05380 | 38.478 | 460 | 247 | 9 | 1 | 448 | 15 | 450 | 7.55e-95 | 294 |
| MS.gene053295.t1 | AT5G17730 | 35.463 | 454 | 261 | 8 | 6 | 450 | 18 | 448 | 1.25e-93 | 291 |
| MS.gene053295.t1 | AT5G17750 | 37.055 | 421 | 211 | 8 | 1 | 410 | 13 | 390 | 5.05e-88 | 274 |
| MS.gene053295.t1 | AT2G46620 | 32.118 | 439 | 236 | 8 | 22 | 445 | 25 | 416 | 6.85e-73 | 238 |
| MS.gene053295.t1 | AT5G17760 | 38.951 | 267 | 149 | 1 | 59 | 311 | 70 | 336 | 8.34e-59 | 197 |
| MS.gene053295.t1 | AT3G28560 | 43.256 | 215 | 104 | 3 | 1 | 204 | 7 | 214 | 5.95e-51 | 174 |
| MS.gene053295.t1 | AT3G29800 | 31.897 | 348 | 182 | 9 | 102 | 442 | 78 | 377 | 2.47e-47 | 169 |
| MS.gene053295.t1 | AT4G05340 | 49.485 | 97 | 39 | 2 | 304 | 397 | 4 | 93 | 1.60e-20 | 86.7 |
| MS.gene053295.t1 | AT3G16290 | 27.979 | 193 | 106 | 5 | 200 | 384 | 415 | 582 | 3.11e-12 | 69.3 |
Find 81 sgRNAs with CRISPR-Local
Find 93 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCACATCTCTATTGTGTTTA+TGG | 0.043514 | 1.4:+2135280 | None:intergenic |
| ACTAGTAATGTTACACTTTC+TGG | 0.065042 | 1.4:-2134724 | MS.gene053295:CDS |
| AAAAGAGGTTATTTACTTTA+TGG | 0.186213 | 1.4:-2135024 | MS.gene053295:CDS |
| ATACTAATAGTGGATCTTAT+TGG | 0.207885 | 1.4:-2135182 | MS.gene053295:CDS |
| AACAAATTATGTGGAGAAAT+TGG | 0.217945 | 1.4:-2134638 | MS.gene053295:CDS |
| TGAATTTCATTGATGGATTA+TGG | 0.245019 | 1.4:-2134696 | MS.gene053295:CDS |
| TTGCTTCCTTGATGTTTGTT+TGG | 0.248657 | 1.4:-2135680 | MS.gene053295:CDS |
| AAACATCAAGGAAGCAATTA+TGG | 0.257321 | 1.4:+2135686 | None:intergenic |
| AAAAGAAGGTAAAGCAATTA+AGG | 0.288836 | 1.4:-2135229 | MS.gene053295:CDS |
| TTTGTGAGTATTTCTCAATT+TGG | 0.291370 | 1.4:+2135619 | None:intergenic |
| CTTTGCTTGTGTTGATGCTT+TGG | 0.296328 | 1.4:+2135488 | None:intergenic |
| AATTTGGTTCCTAAGTTGAT+AGG | 0.297058 | 1.4:+2135635 | None:intergenic |
| TGCTTCCTTGATGTTTGTTT+GGG | 0.301724 | 1.4:-2135679 | MS.gene053295:CDS |
| TGAAACATTAGCAATGGATT+TGG | 0.307810 | 1.4:-2135127 | MS.gene053295:CDS |
| ATTGAATCACATAATTTGTT+TGG | 0.322491 | 1.4:-2134517 | MS.gene053295:CDS |
| GCATCACCAGAAGCAGTCTT+TGG | 0.340573 | 1.4:+2134430 | None:intergenic |
| GGACTATTGAATTTCATTGA+TGG | 0.363024 | 1.4:-2134703 | MS.gene053295:CDS |
| TTGATGCCAAAGACTGCTTC+TGG | 0.363892 | 1.4:-2134436 | MS.gene053295:CDS |
| GCTGCCATGGCAAATTTGTT+AGG | 0.379161 | 1.4:-2134970 | MS.gene053295:CDS |
| GGTGCTAAGGTAATTCTCTA+TGG | 0.385040 | 1.4:+2135509 | None:intergenic |
| TGAACTCATGAAATGTGATT+TGG | 0.386423 | 1.4:+2135568 | None:intergenic |
| GTAATTCTCTATGGAAGAAT+AGG | 0.394421 | 1.4:+2135518 | None:intergenic |
| CTTATTACATTTAGTAAAGC+TGG | 0.395330 | 1.4:-2135078 | MS.gene053295:CDS |
| AGGAGGAAGAGAAGGATTCA+AGG | 0.400269 | 1.4:-2134786 | MS.gene053295:CDS |
| TCTTGAATTGACTGCTGTTA+AGG | 0.402564 | 1.4:-2134932 | MS.gene053295:CDS |
| CTTTATGGTCCTCCAGGGAC+TGG | 0.407910 | 1.4:-2135009 | MS.gene053295:CDS |
| GGTGAGTTCTATGCAAGAAT+TGG | 0.409467 | 1.4:-2135057 | MS.gene053295:CDS |
| GGTGACATGTTTGCTCAAAT+AGG | 0.410316 | 1.4:-2135711 | None:intergenic |
| ATGCAAGAATTGGAAGGGCT+TGG | 0.415600 | 1.4:-2135047 | MS.gene053295:CDS |
| ATTGTTGACTTTCCAGTCCC+TGG | 0.415863 | 1.4:+2134997 | None:intergenic |
| TGGTCAAAGGAGGAAGAAAA+AGG | 0.417013 | 1.4:-2134821 | MS.gene053295:CDS |
| GTGTTGATGCTTTGGTGCTA+AGG | 0.429999 | 1.4:+2135496 | None:intergenic |
| AGGAAACTTTATACTAATAG+TGG | 0.434469 | 1.4:-2135192 | MS.gene053295:CDS |
| AATTCAATGGAGTTAAACTT+TGG | 0.438355 | 1.4:-2135389 | MS.gene053295:CDS |
| ATTGGAAGGGCTTGGAAAAG+AGG | 0.439570 | 1.4:-2135039 | MS.gene053295:CDS |
| TATTTACTTTATGGTCCTCC+AGG | 0.463635 | 1.4:-2135015 | MS.gene053295:CDS |
| AAAGGAGAGAAAGGAAGAGG+AGG | 0.467105 | 1.4:-2134803 | MS.gene053295:CDS |
| AAAGGAAGAGGAGGAAGAGA+AGG | 0.471794 | 1.4:-2134794 | MS.gene053295:CDS |
| GTCAACAATGATTGCTGCCA+TGG | 0.475118 | 1.4:-2134983 | MS.gene053295:CDS |
| AACATTTGAAACATTAGCAA+TGG | 0.477196 | 1.4:-2135133 | MS.gene053295:CDS |
| ATTTGGTTCCTAAGTTGATA+GGG | 0.478933 | 1.4:+2135636 | None:intergenic |
| CTTAATTATGTACTAAAAGA+AGG | 0.479074 | 1.4:-2135243 | MS.gene053295:CDS |
| GATCCTGCATTGGTTAGAAA+AGG | 0.480913 | 1.4:-2134616 | MS.gene053295:CDS |
| CATATAGAGTTGTCATATTG+TGG | 0.481944 | 1.4:-2134580 | MS.gene053295:CDS |
| CAATGGAGTTAAACTTTGGT+GGG | 0.482273 | 1.4:-2135385 | MS.gene053295:CDS |
| TGAAATGTGATTTGGATGTA+AGG | 0.487481 | 1.4:+2135576 | None:intergenic |
| AACAATATGTGAATTGTTGA+AGG | 0.490020 | 1.4:-2134494 | MS.gene053295:CDS |
| AAATGCTCAGCAACATCAGC+TGG | 0.493061 | 1.4:+2134457 | None:intergenic |
| TCAATGGAGTTAAACTTTGG+TGG | 0.498351 | 1.4:-2135386 | MS.gene053295:CDS |
| GATTGCTCACTTGATTTGAC+TGG | 0.504911 | 1.4:-2134841 | MS.gene053295:CDS |
| GTTGTCATATTGTGGATTTG+AGG | 0.509085 | 1.4:-2134572 | MS.gene053295:CDS |
| GAAGTAGGTGATGAATTCAA+TGG | 0.510659 | 1.4:-2135402 | MS.gene053295:CDS |
| ATTTGAGGCATTCAAGATGT+TGG | 0.512524 | 1.4:-2134557 | MS.gene053295:CDS |
| TTTGATTCAAGCACTTGAGT+TGG | 0.513367 | 1.4:-2134389 | MS.gene053295:CDS |
| GGAGGAAGAGAAGGATTCAA+GGG | 0.514417 | 1.4:-2134785 | MS.gene053295:CDS |
| GCTAATGTTTCAAATGTTGA+AGG | 0.514554 | 1.4:+2135138 | None:intergenic |
| AGTTGGCAAAAGAAGAAGCT+AGG | 0.520457 | 1.4:-2134372 | MS.gene053295:CDS |
| TCAAGCACTTGTTCTTAGCA+TGG | 0.533913 | 1.4:-2135436 | MS.gene053295:CDS |
| ATCACATTTCATGAGTTCAC+TGG | 0.535244 | 1.4:-2135564 | MS.gene053295:CDS |
| CACTTGATTTGACTGGTCAA+AGG | 0.548667 | 1.4:-2134834 | MS.gene053295:CDS |
| ATTGGTTAGAAAAGGTAGAA+TGG | 0.558531 | 1.4:-2134608 | MS.gene053295:CDS |
| AGAAGCTAGGGTGAAATCAG+TGG | 0.560095 | 1.4:-2134359 | MS.gene053295:CDS |
| ATGGATGATCATGAAGAAGT+AGG | 0.568240 | 1.4:-2135417 | MS.gene053295:CDS |
| GTTAAACTTTGGTGGGCTAG+TGG | 0.573542 | 1.4:-2135378 | MS.gene053295:CDS |
| AATAGCCCAAACAAACATCA+AGG | 0.574656 | 1.4:+2135674 | None:intergenic |
| GGAATTATGGAGGAAAGAGA+TGG | 0.581168 | 1.4:-2134751 | MS.gene053295:CDS |
| ATTTACTTTATGGTCCTCCA+GGG | 0.585163 | 1.4:-2135014 | MS.gene053295:CDS |
| GGAGAAATTGGATCCTGCAT+TGG | 0.595123 | 1.4:-2134626 | MS.gene053295:CDS |
| AGTTCTATGCAAGAATTGGA+AGG | 0.596527 | 1.4:-2135053 | MS.gene053295:CDS |
| ACACAAGCAAAGAGACTCAA+AGG | 0.600263 | 1.4:-2135477 | MS.gene053295:CDS |
| TTGATTTGACTGGTCAAAGG+AGG | 0.608711 | 1.4:-2134831 | MS.gene053295:CDS |
| GAAATGTGATTTGGATGTAA+GGG | 0.622484 | 1.4:+2135577 | None:intergenic |
| GTTGACTTTCCAGTCCCTGG+AGG | 0.623801 | 1.4:+2135000 | None:intergenic |
| GTTGGCAAAAGAAGAAGCTA+GGG | 0.631329 | 1.4:-2134371 | MS.gene053295:CDS |
| GTTCTATGCAAGAATTGGAA+GGG | 0.632683 | 1.4:-2135052 | MS.gene053295:CDS |
| TTAAGGTGAGAAATAGACAA+AGG | 0.654965 | 1.4:-2135212 | MS.gene053295:CDS |
| ATAGCCTAACAAATTTGCCA+TGG | 0.654970 | 1.4:+2134966 | None:intergenic |
| GGATTATGGTCTGCATGTGG+AGG | 0.679090 | 1.4:-2134682 | MS.gene053295:CDS |
| AAGGATGTTCAAACACAACA+TGG | 0.681122 | 1.4:+2135157 | None:intergenic |
| TTAAGGATAACACTGAGTTG+AGG | 0.695176 | 1.4:-2134915 | MS.gene053295:CDS |
| GATGGATTATGGTCTGCATG+TGG | 0.714258 | 1.4:-2134685 | MS.gene053295:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAAGAGGTTATTTACTTTA+TGG | - | chr1.4:2135020-2135039 | MS.gene053295:CDS | 20.0% |
| !! | CTTAATTATGTACTAAAAGA+AGG | - | chr1.4:2134801-2134820 | MS.gene053295:CDS | 20.0% |
| !!! | AAGTGAAAATGAATTTTGTT+TGG | + | chr1.4:2134703-2134722 | None:intergenic | 20.0% |
| !!! | ATTGAATCACATAATTTGTT+TGG | - | chr1.4:2135527-2135546 | MS.gene053295:CDS | 20.0% |
| ! | AAAAGAAGGTAAAGCAATTA+AGG | - | chr1.4:2134815-2134834 | MS.gene053295:CDS | 25.0% |
| ! | AACAAATTATGTGGAGAAAT+TGG | - | chr1.4:2135406-2135425 | MS.gene053295:CDS | 25.0% |
| ! | AACAATATGTGAATTGTTGA+AGG | - | chr1.4:2135550-2135569 | MS.gene053295:CDS | 25.0% |
| ! | AATTCAATGGAGTTAAACTT+TGG | - | chr1.4:2134655-2134674 | MS.gene053295:CDS | 25.0% |
| ! | AGGAAACTTTATACTAATAG+TGG | - | chr1.4:2134852-2134871 | MS.gene053295:CDS | 25.0% |
| ! | ATCACAACATAGATGAAAAA+AGG | - | chr1.4:2134724-2134743 | MS.gene053295:CDS | 25.0% |
| ! | CTTATTACATTTAGTAAAGC+TGG | - | chr1.4:2134966-2134985 | MS.gene053295:CDS | 25.0% |
| ! | TGAGAAATACTCACAAAAAT+TGG | - | chr1.4:2134431-2134450 | MS.gene053295:CDS | 25.0% |
| ! | TTTGTGAGTATTTCTCAATT+TGG | + | chr1.4:2134428-2134447 | None:intergenic | 25.0% |
| !! | AACATTTGAAACATTAGCAA+TGG | - | chr1.4:2134911-2134930 | MS.gene053295:CDS | 25.0% |
| !! | AGTTTTCACAACAAATTATG+TGG | - | chr1.4:2135397-2135416 | MS.gene053295:CDS | 25.0% |
| !!! | AATTTTTTCCCTATCAACTT+AGG | - | chr1.4:2134400-2134419 | MS.gene053295:CDS | 25.0% |
| !!! | ATACTAATAGTGGATCTTAT+TGG | - | chr1.4:2134862-2134881 | MS.gene053295:CDS | 25.0% |
| !!! | TGAATTTCATTGATGGATTA+TGG | - | chr1.4:2135348-2135367 | MS.gene053295:CDS | 25.0% |
| AAACATCAAGGAAGCAATTA+TGG | + | chr1.4:2134361-2134380 | None:intergenic | 30.0% | |
| ACTAGTAATGTTACACTTTC+TGG | - | chr1.4:2135320-2135339 | MS.gene053295:CDS | 30.0% | |
| ATTGGTTAGAAAAGGTAGAA+TGG | - | chr1.4:2135436-2135455 | MS.gene053295:CDS | 30.0% | |
| CATATAGAGTTGTCATATTG+TGG | - | chr1.4:2135464-2135483 | MS.gene053295:CDS | 30.0% | |
| GCTAATGTTTCAAATGTTGA+AGG | + | chr1.4:2134909-2134928 | None:intergenic | 30.0% | |
| GGACTATTGAATTTCATTGA+TGG | - | chr1.4:2135341-2135360 | MS.gene053295:CDS | 30.0% | |
| GTAATTCTCTATGGAAGAAT+AGG | + | chr1.4:2134529-2134548 | None:intergenic | 30.0% | |
| TCACATCTCTATTGTGTTTA+TGG | + | chr1.4:2134767-2134786 | None:intergenic | 30.0% | |
| TGAACTCATGAAATGTGATT+TGG | + | chr1.4:2134479-2134498 | None:intergenic | 30.0% | |
| TTAAGGTGAGAAATAGACAA+AGG | - | chr1.4:2134832-2134851 | MS.gene053295:CDS | 30.0% | |
| ! | CACAATAGAGATGTGATTTT+AGG | - | chr1.4:2134771-2134790 | MS.gene053295:CDS | 30.0% |
| ! | GAAATGTGATTTGGATGTAA+GGG | + | chr1.4:2134470-2134489 | None:intergenic | 30.0% |
| ! | TGAAACATTAGCAATGGATT+TGG | - | chr1.4:2134917-2134936 | MS.gene053295:CDS | 30.0% |
| ! | TGAAATGTGATTTGGATGTA+AGG | + | chr1.4:2134471-2134490 | None:intergenic | 30.0% |
| !! | AATTTGGTTCCTAAGTTGAT+AGG | + | chr1.4:2134412-2134431 | None:intergenic | 30.0% |
| !! | ATTTGGTTCCTAAGTTGATA+GGG | + | chr1.4:2134411-2134430 | None:intergenic | 30.0% |
| AAGGATGTTCAAACACAACA+TGG | + | chr1.4:2134890-2134909 | None:intergenic | 35.0% | |
| AAGGATTCAAGGGAGAAAAA+AGG | - | chr1.4:2135269-2135288 | MS.gene053295:CDS | 35.0% | |
| AATAGCCCAAACAAACATCA+AGG | + | chr1.4:2134373-2134392 | None:intergenic | 35.0% | |
| AGTTCTATGCAAGAATTGGA+AGG | - | chr1.4:2134991-2135010 | MS.gene053295:CDS | 35.0% | |
| ATAGCCTAACAAATTTGCCA+TGG | + | chr1.4:2135081-2135100 | None:intergenic | 35.0% | |
| ATCACATTTCATGAGTTCAC+TGG | - | chr1.4:2134480-2134499 | MS.gene053295:CDS | 35.0% | |
| ATGGATGATCATGAAGAAGT+AGG | - | chr1.4:2134627-2134646 | MS.gene053295:CDS | 35.0% | |
| ATTTACTTTATGGTCCTCCA+GGG | - | chr1.4:2135030-2135049 | MS.gene053295:CDS | 35.0% | |
| CAATGGAGTTAAACTTTGGT+GGG | - | chr1.4:2134659-2134678 | MS.gene053295:CDS | 35.0% | |
| GAAAAAAGGTGGAATTATGG+AGG | - | chr1.4:2135283-2135302 | MS.gene053295:CDS | 35.0% | |
| GAAGTAGGTGATGAATTCAA+TGG | - | chr1.4:2134642-2134661 | MS.gene053295:CDS | 35.0% | |
| GGAGAAAAAAGGTGGAATTA+TGG | - | chr1.4:2135280-2135299 | MS.gene053295:CDS | 35.0% | |
| GTTCTATGCAAGAATTGGAA+GGG | - | chr1.4:2134992-2135011 | MS.gene053295:CDS | 35.0% | |
| GTTGTCATATTGTGGATTTG+AGG | - | chr1.4:2135472-2135491 | MS.gene053295:CDS | 35.0% | |
| TATTTACTTTATGGTCCTCC+AGG | - | chr1.4:2135029-2135048 | MS.gene053295:CDS | 35.0% | |
| TCAATGGAGTTAAACTTTGG+TGG | - | chr1.4:2134658-2134677 | MS.gene053295:CDS | 35.0% | |
| TCTTGAATTGACTGCTGTTA+AGG | - | chr1.4:2135112-2135131 | MS.gene053295:CDS | 35.0% | |
| TGCTTCCTTGATGTTTGTTT+GGG | - | chr1.4:2134365-2134384 | MS.gene053295:CDS | 35.0% | |
| TTAAGGATAACACTGAGTTG+AGG | - | chr1.4:2135129-2135148 | MS.gene053295:CDS | 35.0% | |
| TTGCTTCCTTGATGTTTGTT+TGG | - | chr1.4:2134364-2134383 | MS.gene053295:CDS | 35.0% | |
| TTTGATTCAAGCACTTGAGT+TGG | - | chr1.4:2135655-2135674 | MS.gene053295:CDS | 35.0% | |
| ! | ATTTGAGGCATTCAAGATGT+TGG | - | chr1.4:2135487-2135506 | MS.gene053295:CDS | 35.0% |
| ACACAAGCAAAGAGACTCAA+AGG | - | chr1.4:2134567-2134586 | MS.gene053295:CDS | 40.0% | |
| AGTTGGCAAAAGAAGAAGCT+AGG | - | chr1.4:2135672-2135691 | MS.gene053295:CDS | 40.0% | |
| GAAAAAGGAGAGAAAGGAAG+AGG | - | chr1.4:2135238-2135257 | MS.gene053295:CDS | 40.0% | |
| GAGGAAGAAAAAGGAGAGAA+AGG | - | chr1.4:2135232-2135251 | MS.gene053295:CDS | 40.0% | |
| GATTCAAGGGAGAAAAAAGG+TGG | - | chr1.4:2135272-2135291 | MS.gene053295:CDS | 40.0% | |
| GGAATTATGGAGGAAAGAGA+TGG | - | chr1.4:2135293-2135312 | MS.gene053295:CDS | 40.0% | |
| GGTGAGTTCTATGCAAGAAT+TGG | - | chr1.4:2134987-2135006 | MS.gene053295:CDS | 40.0% | |
| GGTGCTAAGGTAATTCTCTA+TGG | + | chr1.4:2134538-2134557 | None:intergenic | 40.0% | |
| GTTGGCAAAAGAAGAAGCTA+GGG | - | chr1.4:2135673-2135692 | MS.gene053295:CDS | 40.0% | |
| TCAAGCACTTGTTCTTAGCA+TGG | - | chr1.4:2134608-2134627 | MS.gene053295:CDS | 40.0% | |
| TGGTCAAAGGAGGAAGAAAA+AGG | - | chr1.4:2135223-2135242 | MS.gene053295:CDS | 40.0% | |
| ! | CACTTGATTTGACTGGTCAA+AGG | - | chr1.4:2135210-2135229 | MS.gene053295:CDS | 40.0% |
| ! | CTACCTTTTCTAACCAATGC+AGG | + | chr1.4:2135434-2135453 | None:intergenic | 40.0% |
| ! | CTTTGCTTGTGTTGATGCTT+TGG | + | chr1.4:2134559-2134578 | None:intergenic | 40.0% |
| ! | GATTGCTCACTTGATTTGAC+TGG | - | chr1.4:2135203-2135222 | MS.gene053295:CDS | 40.0% |
| ! | TTGATTTGACTGGTCAAAGG+AGG | - | chr1.4:2135213-2135232 | MS.gene053295:CDS | 40.0% |
| !! | GATCCTGCATTGGTTAGAAA+AGG | - | chr1.4:2135428-2135447 | MS.gene053295:CDS | 40.0% |
| AAAGGAAGAGGAGGAAGAGA+AGG | - | chr1.4:2135250-2135269 | MS.gene053295:CDS | 45.0% | |
| AAAGGAGAGAAAGGAAGAGG+AGG | - | chr1.4:2135241-2135260 | MS.gene053295:CDS | 45.0% | |
| AAATGCTCAGCAACATCAGC+TGG | + | chr1.4:2135590-2135609 | None:intergenic | 45.0% | |
| AGAAGCTAGGGTGAAATCAG+TGG | - | chr1.4:2135685-2135704 | MS.gene053295:CDS | 45.0% | |
| AGGAGGAAGAGAAGGATTCA+AGG | - | chr1.4:2135258-2135277 | MS.gene053295:CDS | 45.0% | |
| ATGCAAGAATTGGAAGGGCT+TGG | - | chr1.4:2134997-2135016 | MS.gene053295:CDS | 45.0% | |
| ATTGGAAGGGCTTGGAAAAG+AGG | - | chr1.4:2135005-2135024 | MS.gene053295:CDS | 45.0% | |
| GGAGAAATTGGATCCTGCAT+TGG | - | chr1.4:2135418-2135437 | MS.gene053295:CDS | 45.0% | |
| GGAGGAAGAGAAGGATTCAA+GGG | - | chr1.4:2135259-2135278 | MS.gene053295:CDS | 45.0% | |
| GTCAACAATGATTGCTGCCA+TGG | - | chr1.4:2135061-2135080 | MS.gene053295:CDS | 45.0% | |
| TTGATGCCAAAGACTGCTTC+TGG | - | chr1.4:2135608-2135627 | MS.gene053295:CDS | 45.0% | |
| ! | GCTGCCATGGCAAATTTGTT+AGG | - | chr1.4:2135074-2135093 | MS.gene053295:CDS | 45.0% |
| !! | ATTGTTGACTTTCCAGTCCC+TGG | + | chr1.4:2135050-2135069 | None:intergenic | 45.0% |
| !! | GATGGATTATGGTCTGCATG+TGG | - | chr1.4:2135359-2135378 | MS.gene053295:CDS | 45.0% |
| !! | GTGTTGATGCTTTGGTGCTA+AGG | + | chr1.4:2134551-2134570 | None:intergenic | 45.0% |
| !! | GTTAAACTTTGGTGGGCTAG+TGG | - | chr1.4:2134666-2134685 | MS.gene053295:CDS | 45.0% |
| GCATCACCAGAAGCAGTCTT+TGG | + | chr1.4:2135617-2135636 | None:intergenic | 50.0% | |
| GGATTATGGTCTGCATGTGG+AGG | - | chr1.4:2135362-2135381 | MS.gene053295:CDS | 50.0% | |
| CTTTATGGTCCTCCAGGGAC+TGG | - | chr1.4:2135035-2135054 | MS.gene053295:CDS | 55.0% | |
| ! | GTTGACTTTCCAGTCCCTGG+AGG | + | chr1.4:2135047-2135066 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.4 | gene | 2134339 | 2135727 | 2134339 | ID=MS.gene053295 |
| chr1.4 | mRNA | 2134339 | 2135727 | 2134339 | ID=MS.gene053295.t1;Parent=MS.gene053295 |
| chr1.4 | exon | 2134339 | 2135727 | 2134339 | ID=MS.gene053295.t1.exon1;Parent=MS.gene053295.t1 |
| chr1.4 | CDS | 2134339 | 2135727 | 2134339 | ID=cds.MS.gene053295.t1;Parent=MS.gene053295.t1 |
| Gene Sequence |
| Protein sequence |
