Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene24585.t1 | XP_003590505.1 | 93.2 | 222 | 15 | 0 | 1 | 222 | 1 | 222 | 1.20E-119 | 439.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene24585.t1 | P08688 | 43.7 | 222 | 117 | 6 | 6 | 220 | 7 | 227 | 6.4e-45 | 182.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene24585.t1 | G7IBL7 | 93.2 | 222 | 15 | 0 | 1 | 222 | 1 | 222 | 8.7e-120 | 439.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene052223 | MS.gene24585 | 0.806398 | 8.39E-50 | -1.69E-46 |
| MS.gene055595 | MS.gene24585 | 0.819225 | 1.30E-52 | -1.69E-46 |
| MS.gene055597 | MS.gene24585 | 0.81835 | 2.05E-52 | -1.69E-46 |
| MS.gene055601 | MS.gene24585 | 0.802616 | 5.16E-49 | -1.69E-46 |
| MS.gene059892 | MS.gene24585 | 0.812951 | 3.27E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene24585.t1 | MTR_1g066530 | 93.243 | 222 | 15 | 0 | 1 | 222 | 1 | 222 | 4.79e-158 | 436 |
| MS.gene24585.t1 | MTR_6g073540 | 50.000 | 230 | 106 | 2 | 1 | 221 | 1 | 230 | 2.96e-75 | 227 |
| MS.gene24585.t1 | MTR_6g072470 | 52.000 | 200 | 90 | 1 | 1 | 194 | 1 | 200 | 4.70e-69 | 211 |
| MS.gene24585.t1 | MTR_6g048620 | 49.099 | 222 | 106 | 5 | 6 | 220 | 5 | 226 | 3.29e-66 | 204 |
| MS.gene24585.t1 | MTR_6g045077 | 45.000 | 220 | 116 | 3 | 4 | 220 | 3 | 220 | 2.55e-64 | 199 |
| MS.gene24585.t1 | MTR_6g048910 | 45.455 | 220 | 113 | 4 | 6 | 220 | 5 | 222 | 8.35e-64 | 197 |
| MS.gene24585.t1 | MTR_0695s0030 | 44.091 | 220 | 118 | 4 | 6 | 220 | 65 | 284 | 2.83e-62 | 197 |
| MS.gene24585.t1 | MTR_8g038100 | 44.545 | 220 | 117 | 4 | 6 | 220 | 281 | 500 | 4.91e-60 | 197 |
| MS.gene24585.t1 | MTR_8g038070 | 44.091 | 220 | 118 | 4 | 6 | 220 | 219 | 438 | 5.46e-60 | 195 |
| MS.gene24585.t1 | MTR_0057s0100 | 40.909 | 220 | 125 | 5 | 6 | 220 | 7 | 226 | 1.67e-53 | 172 |
| MS.gene24585.t1 | MTR_0057s0170 | 40.909 | 220 | 125 | 5 | 6 | 220 | 7 | 226 | 5.23e-53 | 170 |
| MS.gene24585.t1 | MTR_7g103990 | 41.704 | 223 | 120 | 8 | 6 | 220 | 7 | 227 | 9.58e-51 | 164 |
| MS.gene24585.t1 | MTR_6g040290 | 39.910 | 223 | 126 | 6 | 6 | 220 | 7 | 229 | 3.96e-46 | 153 |
| MS.gene24585.t1 | MTR_0807s0020 | 37.054 | 224 | 131 | 5 | 1 | 220 | 1 | 218 | 9.06e-46 | 151 |
| MS.gene24585.t1 | MTR_0216s0030 | 38.889 | 198 | 113 | 3 | 4 | 197 | 1 | 194 | 1.05e-44 | 148 |
| MS.gene24585.t1 | MTR_6g048880 | 41.011 | 178 | 95 | 5 | 50 | 220 | 4 | 178 | 1.69e-40 | 137 |
| MS.gene24585.t1 | MTR_7g012000 | 39.423 | 208 | 96 | 5 | 6 | 209 | 7 | 188 | 1.70e-39 | 135 |
| MS.gene24585.t1 | MTR_6g048810 | 31.699 | 306 | 108 | 9 | 6 | 220 | 5 | 300 | 4.12e-37 | 131 |
| MS.gene24585.t1 | MTR_4g102470 | 41.111 | 180 | 83 | 5 | 6 | 182 | 7 | 166 | 2.03e-34 | 122 |
| MS.gene24585.t1 | MTR_6g049040 | 40.876 | 137 | 64 | 3 | 1 | 135 | 1 | 122 | 5.56e-27 | 100 |
| MS.gene24585.t1 | MTR_6g049040 | 34.188 | 117 | 66 | 2 | 72 | 187 | 16 | 122 | 5.99e-16 | 72.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 46 sgRNAs with CRISPR-Local
Find 51 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTCGGTACGGTCTTTGAAAA+TGG | 0.203555 | 1.3:+46712390 | MS.gene24585:CDS |
| ATCCGGAGAGAAATCTATTT+TGG | 0.256716 | 1.3:-46712157 | None:intergenic |
| TTTAACAACACGATCCTTCT+TGG | 0.279901 | 1.3:-46712013 | None:intergenic |
| ATTAGATCTAGAAGTTTAAT+AGG | 0.298795 | 1.3:-46712563 | None:intergenic |
| ACTCATTACTCCATTCGTTC+TGG | 0.346885 | 1.3:+46712354 | MS.gene24585:CDS |
| AACATGGTAGTGATTGGAAC+AGG | 0.421529 | 1.3:-46712204 | None:intergenic |
| AATGGTGTTTCATCAAGAAA+TGG | 0.425643 | 1.3:-46712063 | None:intergenic |
| GTTACGTGTTCAAGAAGAAA+AGG | 0.446425 | 1.3:+46711962 | MS.gene24585:CDS |
| CAATGGAACATGGTAGTGAT+TGG | 0.462327 | 1.3:-46712210 | None:intergenic |
| CAATCTACTTCCTTCTCAAA+TGG | 0.462912 | 1.3:-46712081 | None:intergenic |
| GAATAAGAAAACATTATGTC+CGG | 0.469058 | 1.3:-46712283 | None:intergenic |
| ACCGAAGCAATCATCTTCTC+TGG | 0.471293 | 1.3:+46712123 | MS.gene24585:CDS |
| TGCAGTTGTGAATTATGCTC+CGG | 0.478704 | 1.3:+46711987 | MS.gene24585:CDS |
| AGAAGTTTAATAGGCTCTGA+AGG | 0.499900 | 1.3:-46712554 | None:intergenic |
| TTTAATAGGCTCTGAAGGGC+AGG | 0.501025 | 1.3:-46712549 | None:intergenic |
| GCCAGAGAAGATGATTGCTT+CGG | 0.505795 | 1.3:-46712124 | None:intergenic |
| AACAGGGCCATCGAGTAAAT+CGG | 0.507747 | 1.3:-46712187 | None:intergenic |
| AGTAAATCGGTATCTGAATC+CGG | 0.514277 | 1.3:-46712174 | None:intergenic |
| GACAATACAGTCACCTTCGT+TGG | 0.516096 | 1.3:-46712493 | None:intergenic |
| GAAGGGCAGGCCATTCCTCA+TGG | 0.517980 | 1.3:-46712536 | None:intergenic |
| ACACGATCCTTCTTGGCTCC+CGG | 0.519674 | 1.3:-46712006 | None:intergenic |
| TGTCAATGGTAGAGTAAAGT+TGG | 0.520266 | 1.3:+46712512 | MS.gene24585:CDS |
| AAACCATCGGAAGTATGAAT+CGG | 0.529141 | 1.3:-46712039 | None:intergenic |
| TTCGGTGCCTTCTGTCTCAA+AGG | 0.532520 | 1.3:-46712106 | None:intergenic |
| AGGAAATGAGTATGTTCGTA+TGG | 0.536361 | 1.3:+46712308 | MS.gene24585:CDS |
| TACCATGTTCCATTGTCTCA+AGG | 0.539896 | 1.3:+46712218 | MS.gene24585:CDS |
| TGCCAAAATAGATTTCTCTC+CGG | 0.543347 | 1.3:+46712155 | MS.gene24585:CDS |
| GAATTGACGCTGCAATTAAG+TGG | 0.544957 | 1.3:+46712259 | MS.gene24585:CDS |
| AAGCCGATTCATACTTCCGA+TGG | 0.550807 | 1.3:+46712036 | MS.gene24585:CDS |
| TTATGCTCCGGGAGCCAAGA+AGG | 0.560419 | 1.3:+46711999 | MS.gene24585:CDS |
| AAAACGTTCCCTTGAGACAA+TGG | 0.561335 | 1.3:-46712227 | None:intergenic |
| TGATGAAACACCATTTGAGA+AGG | 0.562306 | 1.3:+46712071 | MS.gene24585:CDS |
| GAAGGTGACTGTATTGTCAA+TGG | 0.563529 | 1.3:+46712498 | MS.gene24585:CDS |
| ATCAAGAAATGGAAAACCAT+CGG | 0.570603 | 1.3:-46712052 | None:intergenic |
| GATTGTGCCTTTGAGACAGA+AGG | 0.572261 | 1.3:+46712099 | MS.gene24585:CDS |
| GCAGTTGTGAATTATGCTCC+GGG | 0.572517 | 1.3:+46711988 | MS.gene24585:CDS |
| ACCATGTTCCATTGTCTCAA+GGG | 0.576700 | 1.3:+46712219 | MS.gene24585:CDS |
| GACGCTGCAATTAAGTGGAC+CGG | 0.578299 | 1.3:+46712264 | MS.gene24585:CDS |
| GAAATGCAGTATCAATGACT+TGG | 0.591249 | 1.3:-46711918 | None:intergenic |
| TAAAGTTGGTCCATGAGGAA+TGG | 0.593058 | 1.3:+46712526 | MS.gene24585:CDS |
| TCAGATACCGATTTACTCGA+TGG | 0.596226 | 1.3:+46712180 | MS.gene24585:CDS |
| CGTATTGATATTTCCAACGA+AGG | 0.604655 | 1.3:+46712480 | MS.gene24585:CDS |
| GAAGTTTAATAGGCTCTGAA+GGG | 0.628949 | 1.3:-46712553 | None:intergenic |
| TAGAGTAAAGTTGGTCCATG+AGG | 0.649165 | 1.3:+46712521 | MS.gene24585:CDS |
| TCCCTTGAGACAATGGAACA+TGG | 0.652701 | 1.3:-46712220 | None:intergenic |
| ACATGGTAGTGATTGGAACA+GGG | 0.671114 | 1.3:-46712203 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | CATAATGTTTTCTTATTCAA+AGG | + | chr1.3:46712288-46712307 | MS.gene24585:CDS | 20.0% |
| ! | GAATAAGAAAACATTATGTC+CGG | - | chr1.3:46712286-46712305 | None:intergenic | 25.0% |
| AATGGTGTTTCATCAAGAAA+TGG | - | chr1.3:46712066-46712085 | None:intergenic | 30.0% | |
| ATCAAGAAATGGAAAACCAT+CGG | - | chr1.3:46712055-46712074 | None:intergenic | 30.0% | |
| ! | AATCAAGCTTACGTTTTCAA+AGG | + | chr1.3:46712444-46712463 | MS.gene24585:CDS | 30.0% |
| !! | AAGGGAACGTTTTTAGAAAA+TGG | + | chr1.3:46712237-46712256 | MS.gene24585:CDS | 30.0% |
| !! | TTTTAAAAGTTTGCTCGGTA+CGG | + | chr1.3:46712377-46712396 | MS.gene24585:CDS | 30.0% |
| !!! | ACTTTTAAAACCAGAACGAA+TGG | - | chr1.3:46712367-46712386 | None:intergenic | 30.0% |
| !!! | TCTGGTTTTAAAAGTTTGCT+CGG | + | chr1.3:46712372-46712391 | MS.gene24585:CDS | 30.0% |
| AGGAAATGAGTATGTTCGTA+TGG | + | chr1.3:46712308-46712327 | MS.gene24585:CDS | 35.0% | |
| AGTAAATCGGTATCTGAATC+CGG | - | chr1.3:46712177-46712196 | None:intergenic | 35.0% | |
| CAATCTACTTCCTTCTCAAA+TGG | - | chr1.3:46712084-46712103 | None:intergenic | 35.0% | |
| GAAATGCAGTATCAATGACT+TGG | - | chr1.3:46711921-46711940 | None:intergenic | 35.0% | |
| GTTACGTGTTCAAGAAGAAA+AGG | + | chr1.3:46711962-46711981 | MS.gene24585:CDS | 35.0% | |
| TGATGAAACACCATTTGAGA+AGG | + | chr1.3:46712071-46712090 | MS.gene24585:CDS | 35.0% | |
| TGCCAAAATAGATTTCTCTC+CGG | + | chr1.3:46712155-46712174 | MS.gene24585:CDS | 35.0% | |
| TGTCAATGGTAGAGTAAAGT+TGG | + | chr1.3:46712512-46712531 | MS.gene24585:CDS | 35.0% | |
| TTTAACAACACGATCCTTCT+TGG | - | chr1.3:46712016-46712035 | None:intergenic | 35.0% | |
| ! | AAACCATCGGAAGTATGAAT+CGG | - | chr1.3:46712042-46712061 | None:intergenic | 35.0% |
| ! | AGAAGTTTAATAGGCTCTGA+AGG | - | chr1.3:46712557-46712576 | None:intergenic | 35.0% |
| ! | ATCCGGAGAGAAATCTATTT+TGG | - | chr1.3:46712160-46712179 | None:intergenic | 35.0% |
| ! | CGTATTGATATTTCCAACGA+AGG | + | chr1.3:46712480-46712499 | MS.gene24585:CDS | 35.0% |
| !! | GAAGTTTAATAGGCTCTGAA+GGG | - | chr1.3:46712556-46712575 | None:intergenic | 35.0% |
| AAAACGTTCCCTTGAGACAA+TGG | - | chr1.3:46712230-46712249 | None:intergenic | 40.0% | |
| ACCATGTTCCATTGTCTCAA+GGG | + | chr1.3:46712219-46712238 | MS.gene24585:CDS | 40.0% | |
| ACTCATTACTCCATTCGTTC+TGG | + | chr1.3:46712354-46712373 | MS.gene24585:CDS | 40.0% | |
| GAAGGTGACTGTATTGTCAA+TGG | + | chr1.3:46712498-46712517 | MS.gene24585:CDS | 40.0% | |
| GAATTGACGCTGCAATTAAG+TGG | + | chr1.3:46712259-46712278 | MS.gene24585:CDS | 40.0% | |
| TACCATGTTCCATTGTCTCA+AGG | + | chr1.3:46712218-46712237 | MS.gene24585:CDS | 40.0% | |
| TGCAGTTGTGAATTATGCTC+CGG | + | chr1.3:46711987-46712006 | MS.gene24585:CDS | 40.0% | |
| ! | AACATGGTAGTGATTGGAAC+AGG | - | chr1.3:46712207-46712226 | None:intergenic | 40.0% |
| ! | ACATGGTAGTGATTGGAACA+GGG | - | chr1.3:46712206-46712225 | None:intergenic | 40.0% |
| ! | CAATGGAACATGGTAGTGAT+TGG | - | chr1.3:46712213-46712232 | None:intergenic | 40.0% |
| ! | TAAAGTTGGTCCATGAGGAA+TGG | + | chr1.3:46712526-46712545 | MS.gene24585:CDS | 40.0% |
| ! | TAGAGTAAAGTTGGTCCATG+AGG | + | chr1.3:46712521-46712540 | MS.gene24585:CDS | 40.0% |
| !! | TCAGATACCGATTTACTCGA+TGG | + | chr1.3:46712180-46712199 | MS.gene24585:CDS | 40.0% |
| AACAGGGCCATCGAGTAAAT+CGG | - | chr1.3:46712190-46712209 | None:intergenic | 45.0% | |
| ACCGAAGCAATCATCTTCTC+TGG | + | chr1.3:46712123-46712142 | MS.gene24585:CDS | 45.0% | |
| GACAATACAGTCACCTTCGT+TGG | - | chr1.3:46712496-46712515 | None:intergenic | 45.0% | |
| GCAGTTGTGAATTATGCTCC+GGG | + | chr1.3:46711988-46712007 | MS.gene24585:CDS | 45.0% | |
| GCCAGAGAAGATGATTGCTT+CGG | - | chr1.3:46712127-46712146 | None:intergenic | 45.0% | |
| TCCCTTGAGACAATGGAACA+TGG | - | chr1.3:46712223-46712242 | None:intergenic | 45.0% | |
| ! | AAGCCGATTCATACTTCCGA+TGG | + | chr1.3:46712036-46712055 | MS.gene24585:CDS | 45.0% |
| ! | CTCGGTACGGTCTTTGAAAA+TGG | + | chr1.3:46712390-46712409 | MS.gene24585:CDS | 45.0% |
| !! | GATTGTGCCTTTGAGACAGA+AGG | + | chr1.3:46712099-46712118 | MS.gene24585:CDS | 45.0% |
| !! | TTTAATAGGCTCTGAAGGGC+AGG | - | chr1.3:46712552-46712571 | None:intergenic | 45.0% |
| GACGCTGCAATTAAGTGGAC+CGG | + | chr1.3:46712264-46712283 | MS.gene24585:CDS | 50.0% | |
| !! | TTCGGTGCCTTCTGTCTCAA+AGG | - | chr1.3:46712109-46712128 | None:intergenic | 50.0% |
| ACACGATCCTTCTTGGCTCC+CGG | - | chr1.3:46712009-46712028 | None:intergenic | 55.0% | |
| TTATGCTCCGGGAGCCAAGA+AGG | + | chr1.3:46711999-46712018 | MS.gene24585:CDS | 55.0% | |
| GAAGGGCAGGCCATTCCTCA+TGG | - | chr1.3:46712539-46712558 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.3 | gene | 46711910 | 46712578 | 46711910 | ID=MS.gene24585 |
| chr1.3 | mRNA | 46711910 | 46712578 | 46711910 | ID=MS.gene24585.t1;Parent=MS.gene24585 |
| chr1.3 | exon | 46711910 | 46712578 | 46711910 | ID=MS.gene24585.t1.exon1;Parent=MS.gene24585.t1 |
| chr1.3 | CDS | 46711910 | 46712578 | 46711910 | ID=cds.MS.gene24585.t1;Parent=MS.gene24585.t1 |
| Gene Sequence |
| Protein sequence |
