AlfalfaGEDB | MS.gene055595

M. sativa cultivar XinJiangDaYe / MS.gene055595

KEGG: K14686
GO: GO:0000041 GO:0003674 GO:0005215 GO:0005375 GO:0005575 GO:0006810 GO:0006811 GO:0006812 GO:0006825 GO:0008150 GO:0008324 GO:0015075 GO:0015318 GO:0016021 GO:0022857 GO:0022890 GO:0030001 GO:0031224 GO:0034220 GO:0035434 GO:0046873 GO:0046915 GO:0051179 GO:0051234 GO:0055085 GO:0098655 GO:0098660 GO:0098662 GO:0110165
PFAM: PF04145.18

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene055595.t1	XP_003606781.2	98.7	152	2	0	1	152	1	152	1.00E-77	299.3

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene055595.t1	Q8GWP3	53.4	146	55	5	5	144	1	139	1.2e-29	131.0

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene055595.t1	G7JDP2	98.7	152	2	0	1	152	1	152	7.4e-78	299.3

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene055595	MS.gene055597	0.97324	4.29E-136	-1.69E-46
MS.gene055595	MS.gene059043	0.807174	5.75E-50	-1.69E-46
MS.gene055595	MS.gene09768	0.819085	1.40E-52	-1.69E-46
MS.gene055595	MS.gene21380	0.80785	4.13E-50	-1.69E-46
MS.gene055595	MS.gene24583	0.809693	1.67E-50	-1.69E-46
MS.gene055595	MS.gene24585	0.819225	1.30E-52	-1.69E-46
MS.gene055595	MS.gene24862	0.803226	3.86E-49	-1.69E-46
MS.gene055595	MS.gene28548	0.800731	1.26E-48	-1.69E-46
MS.gene055595	MS.gene35459	0.818417	1.98E-52	-1.69E-46
MS.gene055595	MS.gene35460	0.822792	1.96E-53	-1.69E-46
MS.gene055595	MS.gene35690	0.805387	1.37E-49	-1.69E-46
MS.gene055595	MS.gene38338	0.815938	7.15E-52	-1.69E-46
MS.gene055595	MS.gene42096	0.811806	5.82E-51	-1.69E-46
MS.gene055595	MS.gene43162	0.816376	5.71E-52	-1.69E-46
MS.gene055595	MS.gene46230	0.971184	9.14E-133	-1.69E-46
MS.gene055595	MS.gene46272	0.818065	2.38E-52	-1.69E-46
MS.gene055595	MS.gene46275	0.829899	3.98E-55	-1.69E-46
MS.gene055595	MS.gene48169	0.812224	4.72E-51	-1.69E-46
MS.gene055595	MS.gene50068	0.820347	7.20E-53	-1.69E-46
MS.gene055595	MS.gene50069	0.806242	9.05E-50	-1.69E-46
MS.gene055595	MS.gene72924	0.802912	4.48E-49	-1.69E-46
MS.gene055595	MS.gene74553	0.8036	3.23E-49	-1.69E-46
MS.gene055595	MS.gene84373	0.812267	4.62E-51	-1.69E-46
MS.gene055595	MS.gene85750	0.814983	1.17E-51	-1.69E-46
MS.gene055595	MS.gene87043	0.800337	1.51E-48	-1.69E-46
MS.gene055595	MS.gene89772	0.801111	1.05E-48	-1.69E-46
MS.gene055595	MS.gene97335	0.81883	1.60E-52	-1.69E-46
MS.gene055595	MS.gene99544	0.849177	3.82E-60	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene055595.t1	MTR_4g065660	98.684	152	2	0	1	152	1	152	1.00e-108	306
MS.gene055595.t1	MTR_4g064963	81.513	119	21	1	35	152	46	164	2.05e-63	192
MS.gene055595.t1	MTR_4g065123	74.074	135	33	2	9	142	4	137	5.73e-58	177
MS.gene055595.t1	MTR_7g066070	46.154	143	69	4	8	147	2	139	1.57e-36	123
MS.gene055595.t1	MTR_4g019870	46.087	115	61	1	32	145	13	127	1.71e-33	115
MS.gene055595.t1	MTR_0027s0220	51.282	117	54	2	17	130	12	128	5.12e-32	111
MS.gene055595.t1	MTR_1g015000	38.583	127	57	5	31	136	1	127	1.09e-18	77.4
MS.gene055595.t1	MTR_3g105330	35.156	128	59	3	31	134	1	128	1.45e-15	69.3

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene055595.t1	AT2G26975	53.061	147	56	5	5	145	1	140	4.66e-42	137
MS.gene055595.t1	AT5G59030	45.783	166	76	4	1	152	1	166	1.22e-35	121
MS.gene055595.t1	AT3G46900	49.587	121	57	3	36	152	34	154	3.37e-34	117
MS.gene055595.t1	AT5G59040	45.082	122	66	1	30	150	28	149	3.15e-31	109
MS.gene055595.t1	AT2G37925	43.860	114	60	2	30	139	28	141	2.97e-27	99.8
MS.gene055595.t1	AT5G20650	38.462	130	54	4	31	134	1	130	2.10e-16	71.6

Find 43 sgRNAs with CRISPR-Local

Find 45 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TAGGGTTGGCCTAGCTTATT+TGG	0.200727	4.3:+38713916	MS.gene055595:CDS
TTTCAATGGTGGGGTGTTCT+TGG	0.291991	4.3:+38713958	MS.gene055595:CDS
CATTGATTCTCTTTGACAAT+TGG	0.313757	4.3:+38713744	MS.gene055595:CDS
TGATGCACATGACATTCTTC+TGG	0.316430	4.3:+38713711	MS.gene055595:CDS
CTGTGCTTGGTCATGCTGTT+GGG	0.361371	4.3:+38713984	MS.gene055595:CDS
CTCTTTGACAATTGGCCTGC+TGG	0.377320	4.3:+38713752	MS.gene055595:CDS
TGAACTAGACCGGCCACAAC+AGG	0.384941	4.3:-38713875	None:intergenic
GGCCACAACAGGGTTTGAAC+CGG	0.386419	4.3:-38713864	None:intergenic
GCTGTGCTTGGTCATGCTGT+TGG	0.390679	4.3:+38713983	MS.gene055595:CDS
AATTGGCCTGCTGGTAGTTC+TGG	0.393131	4.3:+38713761	MS.gene055595:CDS
CACTCATGTCTTTCAATGGT+GGG	0.393883	4.3:+38713948	MS.gene055595:CDS
TGAGAGAAACTCGATGAGTA+TGG	0.396012	4.3:-38713822	None:intergenic
CCTAGCTTATTTGGTTATGT+TGG	0.401335	4.3:+38713925	MS.gene055595:CDS
TTGAACCGGGTTTAATGAAC+CGG	0.410106	4.3:-38713850	None:intergenic
GTGTTCTTGGTTGCTGTGCT+TGG	0.410298	4.3:+38713971	MS.gene055595:CDS
ACCCATCATCATAGTGTCAT+TGG	0.428445	4.3:-38713675	None:intergenic
TACCAATGACACTATGATGA+TGG	0.457064	4.3:+38713673	MS.gene055595:CDS
TAGTTCTGGTAAGTATGTAT+TGG	0.476161	4.3:+38713775	MS.gene055595:CDS
GATGCACATGACATTCTTCT+GGG	0.480501	4.3:+38713712	MS.gene055595:CDS
GCACTCATGTCTTTCAATGG+TGG	0.488475	4.3:+38713947	MS.gene055595:CDS
GCCACAACAGGGTTTGAACC+GGG	0.512994	4.3:-38713863	None:intergenic
TTGGCACTCATGTCTTTCAA+TGG	0.515628	4.3:+38713944	MS.gene055595:CDS
GGTTCAAACCCTGTTGTGGC+CGG	0.535871	4.3:+38713866	MS.gene055595:CDS
TTGGTAGATGATGATGACTT+TGG	0.542496	4.3:-38713656	None:intergenic
ATGGATCATGACATGGATCA+TGG	0.546244	4.3:+38713620	MS.gene055595:CDS
CCAACATAACCAAATAAGCT+AGG	0.548358	4.3:-38713925	None:intergenic
TCGAAATTCTCATCTTGATG+TGG	0.556567	4.3:-38714037	None:intergenic
ACCCGGTTCAAACCCTGTTG+TGG	0.557595	4.3:+38713862	MS.gene055595:CDS
TCGAGTTTCTCTCAAGCACC+CGG	0.561462	4.3:+38713831	MS.gene055595:CDS
CAAAAGTGTCTGAACTAGAC+CGG	0.563621	4.3:-38713885	None:intergenic
ACCAATGACACTATGATGAT+GGG	0.572192	4.3:+38713674	MS.gene055595:CDS
AGCACCCGGTTCATTAAACC+CGG	0.579765	4.3:+38713845	MS.gene055595:CDS
ACTCATGTCTTTCAATGGTG+GGG	0.593261	4.3:+38713949	MS.gene055595:CDS
TGAACCGGGTTTAATGAACC+GGG	0.596910	4.3:-38713849	None:intergenic
TCATGACATGGATCATGGAA+TGG	0.630684	4.3:+38713625	MS.gene055595:CDS
AATAAGCTAGGCCAACCCTA+AGG	0.634400	4.3:-38713913	None:intergenic
TACTTACCAGAACTACCAGC+AGG	0.638786	4.3:-38713767	None:intergenic
CATGGATCATGGAATGGCAA+TGG	0.655000	4.3:+38713631	MS.gene055595:CDS
AAACTCGATGAGTATGGACA+TGG	0.667480	4.3:-38713816	None:intergenic
GAACTAGACCGGCCACAACA+GGG	0.667595	4.3:-38713874	None:intergenic
AAACAGCATGGATCATGACA+TGG	0.668548	4.3:+38713613	None:intergenic
AATAATTAACAAGATAACGG+AGG	0.670863	4.3:-38714064	None:intergenic
ATGCACATGACATTCTTCTG+GGG	0.703114	4.3:+38713713	MS.gene055595:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	CATTGATTCTCTTTGACAAT+TGG	+	chr4.3:38713744-38713763	MS.gene055595:CDS	30.0%
!!	TAGTTCTGGTAAGTATGTAT+TGG	+	chr4.3:38713775-38713794	MS.gene055595:CDS	30.0%
	ACCAATGACACTATGATGAT+GGG	+	chr4.3:38713674-38713693	MS.gene055595:CDS	35.0%
	CCAACATAACCAAATAAGCT+AGG	-	chr4.3:38713928-38713947	None:intergenic	35.0%
	CCTAGCTTATTTGGTTATGT+TGG	+	chr4.3:38713925-38713944	MS.gene055595:CDS	35.0%
	TACCAATGACACTATGATGA+TGG	+	chr4.3:38713673-38713692	MS.gene055595:CDS	35.0%
	TCGAAATTCTCATCTTGATG+TGG	-	chr4.3:38714040-38714059	None:intergenic	35.0%
!	TTGGTAGATGATGATGACTT+TGG	-	chr4.3:38713659-38713678	None:intergenic	35.0%
!!!	GATGTGGTTTTTTGAAAGCA+CGG	-	chr4.3:38714024-38714043	None:intergenic	35.0%
	AAACTCGATGAGTATGGACA+TGG	-	chr4.3:38713819-38713838	None:intergenic	40.0%
	ACCCATCATCATAGTGTCAT+TGG	-	chr4.3:38713678-38713697	None:intergenic	40.0%
	ACTCATGTCTTTCAATGGTG+GGG	+	chr4.3:38713949-38713968	MS.gene055595:CDS	40.0%
	ATGCACATGACATTCTTCTG+GGG	+	chr4.3:38713713-38713732	MS.gene055595:CDS	40.0%
	ATGGATCATGACATGGATCA+TGG	+	chr4.3:38713620-38713639	MS.gene055595:CDS	40.0%
	CACTCATGTCTTTCAATGGT+GGG	+	chr4.3:38713948-38713967	MS.gene055595:CDS	40.0%
	GATGCACATGACATTCTTCT+GGG	+	chr4.3:38713712-38713731	MS.gene055595:CDS	40.0%
	TCATGACATGGATCATGGAA+TGG	+	chr4.3:38713625-38713644	MS.gene055595:CDS	40.0%
	TGAGAGAAACTCGATGAGTA+TGG	-	chr4.3:38713825-38713844	None:intergenic	40.0%
	TGATGCACATGACATTCTTC+TGG	+	chr4.3:38713711-38713730	MS.gene055595:CDS	40.0%
	TTGAACCGGGTTTAATGAAC+CGG	-	chr4.3:38713853-38713872	None:intergenic	40.0%
	TTGGCACTCATGTCTTTCAA+TGG	+	chr4.3:38713944-38713963	MS.gene055595:CDS	40.0%
!	AGACACTTTTGCATGTCCTT+AGG	+	chr4.3:38713897-38713916	MS.gene055595:CDS	40.0%
!	GACACTTTTGCATGTCCTTA+GGG	+	chr4.3:38713898-38713917	MS.gene055595:CDS	40.0%
!!	CAAAAGTGTCTGAACTAGAC+CGG	-	chr4.3:38713888-38713907	None:intergenic	40.0%
	AATAAGCTAGGCCAACCCTA+AGG	-	chr4.3:38713916-38713935	None:intergenic	45.0%
	CATGGATCATGGAATGGCAA+TGG	+	chr4.3:38713631-38713650	MS.gene055595:CDS	45.0%
	GCACTCATGTCTTTCAATGG+TGG	+	chr4.3:38713947-38713966	MS.gene055595:CDS	45.0%
	TACTTACCAGAACTACCAGC+AGG	-	chr4.3:38713770-38713789	None:intergenic	45.0%
	TGAACCGGGTTTAATGAACC+GGG	-	chr4.3:38713852-38713871	None:intergenic	45.0%
!	CTTTTGCATGTCCTTAGGGT+TGG	+	chr4.3:38713902-38713921	MS.gene055595:CDS	45.0%
!	TTTCAATGGTGGGGTGTTCT+TGG	+	chr4.3:38713958-38713977	MS.gene055595:CDS	45.0%
!!	TAGGGTTGGCCTAGCTTATT+TGG	+	chr4.3:38713916-38713935	MS.gene055595:CDS	45.0%
	AGCACCCGGTTCATTAAACC+CGG	+	chr4.3:38713845-38713864	MS.gene055595:CDS	50.0%
	CTGTGCTTGGTCATGCTGTT+GGG	+	chr4.3:38713984-38714003	MS.gene055595:CDS	50.0%
	GTGTTCTTGGTTGCTGTGCT+TGG	+	chr4.3:38713971-38713990	MS.gene055595:CDS	50.0%
	TCGAGTTTCTCTCAAGCACC+CGG	+	chr4.3:38713831-38713850	MS.gene055595:CDS	50.0%
!	AATTGGCCTGCTGGTAGTTC+TGG	+	chr4.3:38713761-38713780	MS.gene055595:CDS	50.0%
!	CTCTTTGACAATTGGCCTGC+TGG	+	chr4.3:38713752-38713771	MS.gene055595:CDS	50.0%
	ACCCGGTTCAAACCCTGTTG+TGG	+	chr4.3:38713862-38713881	MS.gene055595:CDS	55.0%
	GAACTAGACCGGCCACAACA+GGG	-	chr4.3:38713877-38713896	None:intergenic	55.0%
	GCCACAACAGGGTTTGAACC+GGG	-	chr4.3:38713866-38713885	None:intergenic	55.0%
	GCTGTGCTTGGTCATGCTGT+TGG	+	chr4.3:38713983-38714002	MS.gene055595:CDS	55.0%
	GGCCACAACAGGGTTTGAAC+CGG	-	chr4.3:38713867-38713886	None:intergenic	55.0%
	GGTTCAAACCCTGTTGTGGC+CGG	+	chr4.3:38713866-38713885	MS.gene055595:CDS	55.0%
	TGAACTAGACCGGCCACAAC+AGG	-	chr4.3:38713878-38713897	None:intergenic	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.3	gene	38713620	38714076	38713620	ID=MS.gene055595
chr4.3	mRNA	38713620	38714076	38713620	ID=MS.gene055595.t1;Parent=MS.gene055595
chr4.3	exon	38713620	38714076	38713620	ID=MS.gene055595.t1.exon1;Parent=MS.gene055595.t1
chr4.3	CDS	38713620	38714076	38713620	ID=cds.MS.gene055595.t1;Parent=MS.gene055595.t1

Gene Sequence

>MS.gene055595

ATGGATCATGACATGGATCATGGAATGGCAATGGCACCAAAGTCATCATCATCTACCAATGACACTATGATGATGGGTCAGATGCATGACATGATGCACATGACATTCTTCTGGGGTAAAGATGCATTGATTCTCTTTGACAATTGGCCTGCTGGTAGTTCTGGTAAGTATGTATTGGCTTTGATTCTTGTTTTCGCCATGTCCATACTCATCGAGTTTCTCTCAAGCACCCGGTTCATTAAACCCGGTTCAAACCCTGTTGTGGCCGGTCTAGTTCAGACACTTTTGCATGTCCTTAGGGTTGGCCTAGCTTATTTGGTTATGTTGGCACTCATGTCTTTCAATGGTGGGGTGTTCTTGGTTGCTGTGCTTGGTCATGCTGTTGGGTTCTTCTTTCGTAGCCGTGCTTTCAAAAAACCACATCAAGATGAGAATTTCGATCTTCCTCCGTTATCTT

Protein sequence

>MS.gene055595.t1

MDHDMDHGMAMAPKSSSSTNDTMMMGQMHDMMHMTFFWGKDALILFDNWPAGSSGKYVLALILVFAMSILIEFLSSTRFIKPGSNPVVAGLVQTLLHVLRVGLAYLVMLALMSFNGGVFLVAVLGHAVGFFFRSRAFKKPHQDENFDLPPLS