AlfalfaGEDB | MS.gene055597

M. sativa cultivar XinJiangDaYe / MS.gene055597

KEGG: K14686
GO: GO:0000041 GO:0003674 GO:0005215 GO:0005375 GO:0005575 GO:0006810 GO:0006811 GO:0006812 GO:0006825 GO:0008150 GO:0008324 GO:0015075 GO:0015318 GO:0016021 GO:0022857 GO:0022890 GO:0030001 GO:0031224 GO:0034220 GO:0035434 GO:0046873 GO:0046915 GO:0051179 GO:0051234 GO:0055085 GO:0098655 GO:0098660 GO:0098662 GO:0110165
PFAM: PF04145.18

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene055597.t1	XP_003606781.2	98.7	153	2	0	1	153	1	153	5.50E-79	303.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene055597.t1	Q8GWP3	53.4	146	55	5	5	144	1	139	9.0e-30	131.3

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene055597.t1	G7JDP2	98.7	153	2	0	1	153	1	153	3.9e-79	303.5

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene055595	MS.gene055597	0.97324	4.29E-136	-1.69E-46
MS.gene055597	MS.gene056899	0.800593	1.34E-48	-1.69E-46
MS.gene055597	MS.gene21380	0.800562	1.36E-48	-1.69E-46
MS.gene055597	MS.gene24583	0.810291	1.24E-50	-1.69E-46
MS.gene055597	MS.gene24585	0.81835	2.05E-52	-1.69E-46
MS.gene055597	MS.gene28548	0.808259	3.38E-50	-1.69E-46
MS.gene055597	MS.gene35459	0.808307	3.30E-50	-1.69E-46
MS.gene055597	MS.gene35460	0.819449	1.15E-52	-1.69E-46
MS.gene055597	MS.gene35690	0.809868	1.53E-50	-1.69E-46
MS.gene055597	MS.gene38338	0.810306	1.23E-50	-1.69E-46
MS.gene055597	MS.gene42096	0.813892	2.03E-51	-1.69E-46
MS.gene055597	MS.gene43162	0.806396	8.39E-50	-1.69E-46
MS.gene055597	MS.gene46230	0.963806	1.54E-122	-1.69E-46
MS.gene055597	MS.gene46272	0.804519	2.08E-49	-1.69E-46
MS.gene055597	MS.gene46275	0.816259	6.07E-52	-1.69E-46
MS.gene055597	MS.gene48169	0.812985	3.22E-51	-1.69E-46
MS.gene055597	MS.gene50068	0.80877	2.63E-50	-1.69E-46
MS.gene055597	MS.gene70064	0.810373	1.19E-50	-1.69E-46
MS.gene055597	MS.gene72924	0.800674	1.29E-48	-1.69E-46
MS.gene055597	MS.gene84373	0.815305	9.90E-52	-1.69E-46
MS.gene055597	MS.gene85750	0.815177	1.06E-51	-1.69E-46
MS.gene055597	MS.gene87043	0.80515	1.53E-49	-1.69E-46
MS.gene055597	MS.gene89772	0.810599	1.06E-50	-1.69E-46
MS.gene055597	MS.gene97335	0.810844	9.42E-51	-1.69E-46
MS.gene055597	MS.gene99544	0.843624	1.25E-58	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene055597.t1	MTR_4g065660	98.693	153	2	0	1	153	1	153	3.36e-110	310
MS.gene055597.t1	MTR_4g064963	81.667	120	21	1	35	153	46	165	1.23e-64	195
MS.gene055597.t1	MTR_4g065123	74.074	135	33	2	9	142	4	137	7.33e-58	177
MS.gene055597.t1	MTR_7g066070	46.154	143	69	4	8	147	2	139	1.61e-36	123
MS.gene055597.t1	MTR_4g019870	46.087	115	61	1	32	145	13	127	1.48e-33	115
MS.gene055597.t1	MTR_0027s0220	51.282	117	54	2	17	130	12	128	6.52e-32	111
MS.gene055597.t1	MTR_1g015000	38.583	127	57	5	31	136	1	127	1.15e-18	77.4
MS.gene055597.t1	MTR_3g105330	35.156	128	59	3	31	134	1	128	1.43e-15	69.3

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene055597.t1	AT2G26975	53.061	147	56	5	5	145	1	140	6.48e-42	137
MS.gene055597.t1	AT5G59030	45.833	168	76	5	1	153	1	168	1.47e-36	124
MS.gene055597.t1	AT3G46900	49.587	121	57	3	36	152	34	154	4.16e-34	117
MS.gene055597.t1	AT5G59040	45.082	122	66	1	30	150	28	149	4.57e-31	109
MS.gene055597.t1	AT2G37925	43.860	114	60	2	30	139	28	141	3.57e-27	99.4
MS.gene055597.t1	AT5G20650	38.462	130	54	4	31	134	1	130	2.36e-16	71.6

Find 43 sgRNAs with CRISPR-Local

Find 45 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TAGGGTTGGCCTAGCTTATT+TGG	0.200727	4.2:-40053224	MS.gene055597:CDS
TTTCAATGGTGGGGTGTTCT+TGG	0.291991	4.2:-40053182	MS.gene055597:CDS
CATTGATTCTCTTTGACAAT+TGG	0.313757	4.2:-40053396	MS.gene055597:CDS
TGATGCACATGACATTCTTC+TGG	0.316430	4.2:-40053429	MS.gene055597:CDS
CTGTGCTTGGTCATGCTGTT+GGG	0.361371	4.2:-40053156	MS.gene055597:CDS
CTCTTTGACAATTGGCCTGC+TGG	0.377320	4.2:-40053388	MS.gene055597:CDS
TGAACTAGACCGGCCACAAC+AGG	0.384941	4.2:+40053265	None:intergenic
GGCCACAACAGGGTTTGAAC+CGG	0.386419	4.2:+40053276	None:intergenic
GCTGTGCTTGGTCATGCTGT+TGG	0.390679	4.2:-40053157	MS.gene055597:CDS
AATTGGCCTGCTGGTAGTTC+TGG	0.393131	4.2:-40053379	MS.gene055597:CDS
CACTCATGTCTTTCAATGGT+GGG	0.393883	4.2:-40053192	MS.gene055597:CDS
TGAGAGAAACTCGATGAGTA+TGG	0.396012	4.2:+40053318	None:intergenic
CCTAGCTTATTTGGTTATGT+TGG	0.401335	4.2:-40053215	MS.gene055597:CDS
TTGAACCGGGTTTAATGAAC+CGG	0.410106	4.2:+40053290	None:intergenic
GTGTTCTTGGTTGCTGTGCT+TGG	0.410298	4.2:-40053169	MS.gene055597:CDS
ACCCATCATCATAGTGTCAT+TGG	0.428445	4.2:+40053465	None:intergenic
TACCAATGACACTATGATGA+TGG	0.457064	4.2:-40053467	MS.gene055597:CDS
TAGTTCTGGTAAGTATGTAT+TGG	0.476161	4.2:-40053365	MS.gene055597:CDS
GATGCACATGACATTCTTCT+GGG	0.480501	4.2:-40053428	MS.gene055597:CDS
GCACTCATGTCTTTCAATGG+TGG	0.488475	4.2:-40053193	MS.gene055597:CDS
GCCACAACAGGGTTTGAACC+GGG	0.512994	4.2:+40053277	None:intergenic
TTGGCACTCATGTCTTTCAA+TGG	0.515628	4.2:-40053196	MS.gene055597:CDS
GGTTCAAACCCTGTTGTGGC+CGG	0.535871	4.2:-40053274	MS.gene055597:CDS
TTGGTAGATGATGATGACTT+TGG	0.542496	4.2:+40053484	None:intergenic
ATGGATCATGACATGGATCA+TGG	0.546244	4.2:-40053520	MS.gene055597:CDS
CCAACATAACCAAATAAGCT+AGG	0.548358	4.2:+40053215	None:intergenic
TCGAAATTCTCATCTTGATG+TGG	0.556567	4.2:+40053103	None:intergenic
ACCCGGTTCAAACCCTGTTG+TGG	0.557595	4.2:-40053278	MS.gene055597:CDS
TCGAGTTTCTCTCAAGCACC+CGG	0.561462	4.2:-40053309	MS.gene055597:CDS
CAAAAGTGTCTGAACTAGAC+CGG	0.563621	4.2:+40053255	None:intergenic
ACCAATGACACTATGATGAT+GGG	0.572192	4.2:-40053466	MS.gene055597:CDS
AGCACCCGGTTCATTAAACC+CGG	0.579765	4.2:-40053295	MS.gene055597:CDS
ACTCATGTCTTTCAATGGTG+GGG	0.593261	4.2:-40053191	MS.gene055597:CDS
TGAACCGGGTTTAATGAACC+GGG	0.596910	4.2:+40053291	None:intergenic
TCATGACATGGATCATGGAA+TGG	0.630684	4.2:-40053515	MS.gene055597:CDS
AATAAGCTAGGCCAACCCTA+AGG	0.634400	4.2:+40053227	None:intergenic
TACTTACCAGAACTACCAGC+AGG	0.638786	4.2:+40053373	None:intergenic
CATGGATCATGGAATGGCAA+TGG	0.655000	4.2:-40053509	MS.gene055597:CDS
AAACTCGATGAGTATGGACA+TGG	0.667480	4.2:+40053324	None:intergenic
GAACTAGACCGGCCACAACA+GGG	0.667595	4.2:+40053266	None:intergenic
AAACAGCATGGATCATGACA+TGG	0.668548	4.2:-40053527	None:intergenic
AATAATTAACAAGATAACGG+AGG	0.670863	4.2:+40053076	None:intergenic
ATGCACATGACATTCTTCTG+GGG	0.703114	4.2:-40053427	MS.gene055597:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	CATTGATTCTCTTTGACAAT+TGG	-	chr4.2:40053205-40053224	MS.gene055597:CDS	30.0%
!!	TAGTTCTGGTAAGTATGTAT+TGG	-	chr4.2:40053236-40053255	MS.gene055597:CDS	30.0%
	ACCAATGACACTATGATGAT+GGG	-	chr4.2:40053135-40053154	MS.gene055597:CDS	35.0%
	CCAACATAACCAAATAAGCT+AGG	+	chr4.2:40053389-40053408	None:intergenic	35.0%
	CCTAGCTTATTTGGTTATGT+TGG	-	chr4.2:40053386-40053405	MS.gene055597:CDS	35.0%
	TACCAATGACACTATGATGA+TGG	-	chr4.2:40053134-40053153	MS.gene055597:CDS	35.0%
	TCGAAATTCTCATCTTGATG+TGG	+	chr4.2:40053501-40053520	None:intergenic	35.0%
!	TTGGTAGATGATGATGACTT+TGG	+	chr4.2:40053120-40053139	None:intergenic	35.0%
!!!	GATGTGGTTTTTTGAAAGCA+CGG	+	chr4.2:40053485-40053504	None:intergenic	35.0%
	AAACTCGATGAGTATGGACA+TGG	+	chr4.2:40053280-40053299	None:intergenic	40.0%
	ACCCATCATCATAGTGTCAT+TGG	+	chr4.2:40053139-40053158	None:intergenic	40.0%
	ACTCATGTCTTTCAATGGTG+GGG	-	chr4.2:40053410-40053429	MS.gene055597:CDS	40.0%
	ATGCACATGACATTCTTCTG+GGG	-	chr4.2:40053174-40053193	MS.gene055597:CDS	40.0%
	ATGGATCATGACATGGATCA+TGG	-	chr4.2:40053081-40053100	MS.gene055597:CDS	40.0%
	CACTCATGTCTTTCAATGGT+GGG	-	chr4.2:40053409-40053428	MS.gene055597:CDS	40.0%
	GATGCACATGACATTCTTCT+GGG	-	chr4.2:40053173-40053192	MS.gene055597:CDS	40.0%
	TCATGACATGGATCATGGAA+TGG	-	chr4.2:40053086-40053105	MS.gene055597:CDS	40.0%
	TGAGAGAAACTCGATGAGTA+TGG	+	chr4.2:40053286-40053305	None:intergenic	40.0%
	TGATGCACATGACATTCTTC+TGG	-	chr4.2:40053172-40053191	MS.gene055597:CDS	40.0%
	TTGAACCGGGTTTAATGAAC+CGG	+	chr4.2:40053314-40053333	None:intergenic	40.0%
	TTGGCACTCATGTCTTTCAA+TGG	-	chr4.2:40053405-40053424	MS.gene055597:CDS	40.0%
!	AGACACTTTTGCATGTCCTT+AGG	-	chr4.2:40053358-40053377	MS.gene055597:CDS	40.0%
!	GACACTTTTGCATGTCCTTA+GGG	-	chr4.2:40053359-40053378	MS.gene055597:CDS	40.0%
!!	CAAAAGTGTCTGAACTAGAC+CGG	+	chr4.2:40053349-40053368	None:intergenic	40.0%
	AATAAGCTAGGCCAACCCTA+AGG	+	chr4.2:40053377-40053396	None:intergenic	45.0%
	CATGGATCATGGAATGGCAA+TGG	-	chr4.2:40053092-40053111	MS.gene055597:CDS	45.0%
	GCACTCATGTCTTTCAATGG+TGG	-	chr4.2:40053408-40053427	MS.gene055597:CDS	45.0%
	TACTTACCAGAACTACCAGC+AGG	+	chr4.2:40053231-40053250	None:intergenic	45.0%
	TGAACCGGGTTTAATGAACC+GGG	+	chr4.2:40053313-40053332	None:intergenic	45.0%
!	CTTTTGCATGTCCTTAGGGT+TGG	-	chr4.2:40053363-40053382	MS.gene055597:CDS	45.0%
!	TTTCAATGGTGGGGTGTTCT+TGG	-	chr4.2:40053419-40053438	MS.gene055597:CDS	45.0%
!!	TAGGGTTGGCCTAGCTTATT+TGG	-	chr4.2:40053377-40053396	MS.gene055597:CDS	45.0%
	AGCACCCGGTTCATTAAACC+CGG	-	chr4.2:40053306-40053325	MS.gene055597:CDS	50.0%
	CTGTGCTTGGTCATGCTGTT+GGG	-	chr4.2:40053445-40053464	MS.gene055597:CDS	50.0%
	GTGTTCTTGGTTGCTGTGCT+TGG	-	chr4.2:40053432-40053451	MS.gene055597:CDS	50.0%
	TCGAGTTTCTCTCAAGCACC+CGG	-	chr4.2:40053292-40053311	MS.gene055597:CDS	50.0%
!	AATTGGCCTGCTGGTAGTTC+TGG	-	chr4.2:40053222-40053241	MS.gene055597:CDS	50.0%
!	CTCTTTGACAATTGGCCTGC+TGG	-	chr4.2:40053213-40053232	MS.gene055597:CDS	50.0%
	ACCCGGTTCAAACCCTGTTG+TGG	-	chr4.2:40053323-40053342	MS.gene055597:CDS	55.0%
	GAACTAGACCGGCCACAACA+GGG	+	chr4.2:40053338-40053357	None:intergenic	55.0%
	GCCACAACAGGGTTTGAACC+GGG	+	chr4.2:40053327-40053346	None:intergenic	55.0%
	GCTGTGCTTGGTCATGCTGT+TGG	-	chr4.2:40053444-40053463	MS.gene055597:CDS	55.0%
	GGCCACAACAGGGTTTGAAC+CGG	+	chr4.2:40053328-40053347	None:intergenic	55.0%
	GGTTCAAACCCTGTTGTGGC+CGG	-	chr4.2:40053327-40053346	MS.gene055597:CDS	55.0%
	TGAACTAGACCGGCCACAAC+AGG	+	chr4.2:40053339-40053358	None:intergenic	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.2	gene	40053081	40053542	40053081	ID=MS.gene055597
chr4.2	mRNA	40053081	40053542	40053081	ID=MS.gene055597.t1;Parent=MS.gene055597
chr4.2	exon	40053081	40053542	40053081	ID=MS.gene055597.t1.exon1;Parent=MS.gene055597.t1
chr4.2	CDS	40053081	40053542	40053081	ID=cds.MS.gene055597.t1;Parent=MS.gene055597.t1

Gene Sequence

>MS.gene055597

ATGGATCATGACATGGATCATGGAATGGCAATGGCACCAAAGTCATCATCATCTACCAATGACACTATGATGATGGGTCAGATGCATGACATGATGCACATGACATTCTTCTGGGGTAAAGATGCATTGATTCTCTTTGACAATTGGCCTGCTGGTAGTTCTGGTAAGTATGTATTGGCTTTGATTCTTGTTTTCGCCATGTCCATACTCATCGAGTTTCTCTCAAGCACCCGGTTCATTAAACCCGGTTCAAACCCTGTTGTGGCCGGTCTAGTTCAGACACTTTTGCATGTCCTTAGGGTTGGCCTAGCTTATTTGGTTATGTTGGCACTCATGTCTTTCAATGGTGGGGTGTTCTTGGTTGCTGTGCTTGGTCATGCTGTTGGGTTCTTCTTTCGTAGCCGTGCTTTCAAAAAACCACATCAAGATGAGAATTTCGATCTTCCTCCGTTATCTTGTTAA

Protein sequence

>MS.gene055597.t1

MDHDMDHGMAMAPKSSSSTNDTMMMGQMHDMMHMTFFWGKDALILFDNWPAGSSGKYVLALILVFAMSILIEFLSSTRFIKPGSNPVVAGLVQTLLHVLRVGLAYLVMLALMSFNGGVFLVAVLGHAVGFFFRSRAFKKPHQDENFDLPPLSC