AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene25479

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene25479.t1 MTR_8g055840 99.344 610 4 0 1 610 1 610 0.0 1270
MS.gene25479.t1 MTR_5g040360 84.590 610 93 1 1 610 1 609 0.0 1108
MS.gene25479.t1 MTR_3g040370 71.757 609 166 4 2 609 3 606 0.0 946
MS.gene25479.t1 MTR_4g098890 68.874 604 174 5 2 597 3 600 0.0 912
MS.gene25479.t1 MTR_8g100075 54.160 613 251 6 17 608 25 628 0.0 712
MS.gene25479.t1 MTR_7g076170 55.263 608 245 6 17 601 23 626 0.0 711
MS.gene25479.t1 MTR_0019s0120 54.803 635 247 9 3 609 13 635 0.0 708
MS.gene25479.t1 MTR_1g101370 57.383 596 240 8 18 601 25 618 0.0 708
MS.gene25479.t1 MTR_8g432680 52.883 607 262 6 17 601 27 631 0.0 680
MS.gene25479.t1 MTR_7g006060 52.964 523 234 4 84 594 43 565 0.0 585
MS.gene25479.t1 MTR_0019s0120 52.809 534 212 9 3 508 13 534 0.0 562
MS.gene25479.t1 MTR_8g071280 45.660 530 278 7 84 607 70 595 2.50e-166 488
MS.gene25479.t1 MTR_8g432620 44.821 531 265 8 82 600 86 600 5.63e-164 481
MS.gene25479.t1 MTR_7g076150 45.018 542 270 8 71 600 62 587 2.19e-163 479
MS.gene25479.t1 MTR_5g026930 42.833 600 313 10 9 600 13 590 4.46e-163 479
MS.gene25479.t1 MTR_5g080720 43.178 535 279 11 75 597 116 637 8.46e-154 457
MS.gene25479.t1 MTR_1g442840 41.096 584 302 12 20 575 18 587 3.63e-150 447
MS.gene25479.t1 MTR_1g075900 42.755 559 286 12 73 610 79 624 9.48e-148 441
MS.gene25479.t1 MTR_3g068990 44.465 533 263 12 82 598 151 666 1.30e-147 442
MS.gene25479.t1 MTR_1g069785 42.910 536 273 10 87 602 65 587 1.75e-147 439
MS.gene25479.t1 MTR_1g442840 45.607 478 234 9 111 575 10 474 1.28e-146 434
MS.gene25479.t1 MTR_1g029730 44.234 529 271 12 84 599 141 658 4.11e-145 435
MS.gene25479.t1 MTR_7g076150 45.534 459 222 8 154 600 2 444 6.21e-139 412
MS.gene25479.t1 MTR_5g080720 43.061 490 248 12 63 534 98 574 1.45e-135 408
MS.gene25479.t1 MTR_5g026930 42.913 508 261 10 9 508 13 499 5.76e-133 399
MS.gene25479.t1 MTR_3g096480 41.712 549 280 18 73 600 247 776 6.45e-132 405
MS.gene25479.t1 MTR_5g083150 39.388 523 279 13 92 600 100 598 2.02e-130 395
MS.gene25479.t1 MTR_3g071530 38.803 518 285 11 92 600 152 646 9.18e-127 387
MS.gene25479.t1 MTR_8g093880 41.292 511 267 14 93 584 298 794 1.30e-126 392
MS.gene25479.t1 MTR_8g076650 41.811 519 264 15 93 591 387 887 1.40e-125 392
MS.gene25479.t1 MTR_5g021100 40.406 542 282 16 75 591 305 830 1.52e-125 390
MS.gene25479.t1 MTR_1g051055 38.795 531 292 12 87 600 89 603 1.18e-122 375
MS.gene25479.t1 MTR_3g088790 40.076 529 279 17 92 600 232 742 2.08e-121 377
MS.gene25479.t1 MTR_4g083030 32.065 552 333 11 70 599 72 603 2.62e-93 299
MS.gene25479.t1 MTR_1g022255 33.026 542 312 16 76 600 192 699 1.31e-90 295
MS.gene25479.t1 MTR_3g074780 30.648 571 344 14 70 605 140 693 1.31e-89 292
MS.gene25479.t1 MTR_5g064080 30.212 566 333 14 70 600 132 670 2.05e-84 278
MS.gene25479.t1 MTR_1g022255 28.186 408 248 12 76 467 192 570 2.71e-45 169
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene25479.t1 AT1G26850 80.163 615 113 3 3 610 4 616 0.0 1057
MS.gene25479.t1 AT1G26850 80.163 615 113 3 3 610 4 616 0.0 1057
MS.gene25479.t1 AT4G18030 68.272 602 180 4 7 602 9 605 0.0 881
MS.gene25479.t1 AT1G26850 79.000 500 98 2 3 495 4 503 0.0 851
MS.gene25479.t1 AT4G10440 55.122 615 247 5 7 601 12 617 0.0 727
MS.gene25479.t1 AT1G33170 53.155 634 253 6 5 601 9 635 0.0 713
MS.gene25479.t1 AT2G45750 54.058 616 253 9 17 608 19 628 0.0 684
MS.gene25479.t1 AT4G00750 51.809 608 279 4 16 609 24 631 0.0 677
MS.gene25479.t1 AT2G43200 52.031 517 242 4 84 595 91 606 0.0 567
MS.gene25479.t1 AT2G43200 52.031 517 242 4 84 595 91 606 0.0 567
MS.gene25479.t1 AT1G31850 42.114 615 328 14 2 607 8 603 7.67e-173 504
MS.gene25479.t1 AT1G31850 42.114 615 328 14 2 607 8 603 7.67e-173 504
MS.gene25479.t1 AT1G31850 42.114 615 328 14 2 607 8 603 7.67e-173 504
MS.gene25479.t1 AT1G31850 42.114 615 328 14 2 607 8 603 7.67e-173 504
MS.gene25479.t1 AT4G19120 43.942 553 295 9 69 610 52 600 2.43e-169 495
MS.gene25479.t1 AT4G19120 43.942 553 295 9 69 610 52 600 2.43e-169 495
MS.gene25479.t1 AT4G00740 43.049 597 302 11 22 606 29 599 9.25e-165 484
MS.gene25479.t1 AT4G00740 43.049 597 302 11 22 606 29 599 9.25e-165 484
MS.gene25479.t1 AT5G06050 44.090 533 271 12 80 599 145 663 4.10e-150 449
MS.gene25479.t1 AT1G04430 41.799 567 287 12 64 602 64 615 1.49e-145 435
MS.gene25479.t1 AT1G04430 41.799 567 287 12 64 602 64 615 1.49e-145 435
MS.gene25479.t1 AT1G04430 41.799 567 287 12 64 602 64 615 1.49e-145 435
MS.gene25479.t1 AT2G39750 41.423 548 292 11 69 598 154 690 1.52e-145 437
MS.gene25479.t1 AT4G14360 43.444 511 259 10 82 575 74 571 3.44e-143 429
MS.gene25479.t1 AT4G14360 43.444 511 259 10 82 575 74 571 3.44e-143 429
MS.gene25479.t1 AT3G23300 42.329 541 278 12 82 602 77 603 8.57e-143 427
MS.gene25479.t1 AT3G23300 42.329 541 278 12 82 602 77 603 8.57e-143 427
MS.gene25479.t1 AT1G77260 40.418 574 304 14 48 598 96 654 2.26e-140 423
MS.gene25479.t1 AT4G19120 44.789 451 235 8 69 508 52 499 3.53e-139 414
MS.gene25479.t1 AT5G14430 39.234 627 331 16 10 608 8 612 9.29e-137 412
MS.gene25479.t1 AT5G14430 38.915 627 333 16 10 608 8 612 3.70e-135 408
MS.gene25479.t1 AT2G34300 41.714 525 272 13 93 600 250 757 8.75e-130 399
MS.gene25479.t1 AT2G34300 41.714 525 272 13 93 600 250 757 8.75e-130 399
MS.gene25479.t1 AT2G34300 41.714 525 272 13 93 600 250 757 8.75e-130 399
MS.gene25479.t1 AT5G04060 40.076 529 285 11 87 600 86 597 1.38e-129 393
MS.gene25479.t1 AT1G29470 41.714 525 272 14 93 600 250 757 5.32e-129 397
MS.gene25479.t1 AT1G29470 41.714 525 272 14 93 600 250 757 5.32e-129 397
MS.gene25479.t1 AT5G64030 42.095 506 262 14 93 580 308 800 5.71e-129 399
MS.gene25479.t1 AT3G10200 39.474 532 286 13 87 600 75 588 4.73e-127 387
MS.gene25479.t1 AT3G56080 38.921 519 282 11 93 600 114 608 2.85e-126 385
MS.gene25479.t1 AT3G51070 39.798 593 302 20 36 600 324 889 3.63e-126 393
MS.gene25479.t1 AT2G40280 37.524 525 286 12 89 600 86 581 6.41e-126 384
MS.gene25479.t1 AT3G10200 38.921 519 275 14 74 573 67 562 3.92e-118 363
MS.gene25479.t1 AT3G56080 40.625 448 239 10 93 529 114 545 2.43e-112 347
MS.gene25479.t1 AT3G51070 40.962 520 260 18 36 529 324 822 4.24e-109 347
MS.gene25479.t1 AT1G13860 34.522 533 297 14 82 585 79 588 1.74e-95 305
MS.gene25479.t1 AT1G13860 34.522 533 297 14 82 585 79 588 1.74e-95 305
MS.gene25479.t1 AT1G13860 34.522 533 297 14 82 585 79 588 1.74e-95 305
MS.gene25479.t1 AT1G13860 34.522 533 297 14 82 585 79 588 1.74e-95 305
MS.gene25479.t1 AT2G03480 30.990 626 347 16 10 599 16 592 1.17e-91 295
MS.gene25479.t1 AT2G03480 31.200 625 358 16 10 599 16 603 9.61e-91 293
MS.gene25479.t1 AT1G13860 37.188 441 239 12 171 585 4 432 5.21e-88 281
MS.gene25479.t1 AT1G19430 31.955 532 317 15 84 600 221 722 1.51e-85 282
MS.gene25479.t1 AT1G19430 31.955 532 317 15 84 600 221 722 1.51e-85 282
MS.gene25479.t1 AT1G78240 30.742 566 337 14 70 599 132 678 3.44e-83 275
MS.gene25479.t1 AT1G78240 30.742 566 337 14 70 599 132 678 3.44e-83 275
MS.gene25479.t1 AT2G03480 31.461 356 219 7 57 395 52 399 4.06e-50 179
MS.gene25479.t1 AT1G19430 27.960 397 247 12 87 469 224 595 4.23e-43 164

Find 161 sgRNAs with CRISPR-Local

Find 278 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGTGGTTATTGGGTGCTTTC+TGG 0.105198 8.3:-43031023 MS.gene25479:CDS
TGATGGATATGGCAACTTTA+TGG 0.168125 8.3:+43031248 None:intergenic
TATCAGCACCTTGAGGAAAT+TGG 0.176986 8.3:+43031856 None:intergenic
TGCGAGTCAAAACTTAAATT+TGG 0.190280 8.3:+43032227 None:intergenic
TGCTGTTTCTTCTATATATT+GGG 0.197614 8.3:-43032332 MS.gene25479:CDS
GTGCTGTTTCTTCTATATAT+TGG 0.235126 8.3:-43032333 MS.gene25479:CDS
ACTCCGACTCCTAAAGTTTC+TGG 0.236262 8.3:-43030143 MS.gene25479:CDS
TTCTAAGGCCTGGTGGTTAT+TGG 0.265507 8.3:-43031034 MS.gene25479:CDS
CAAGTTGGGGTGCATATCTT+TGG 0.273541 8.3:-43031377 MS.gene25479:CDS
TCTAAGGCCTGGTGGTTATT+GGG 0.298345 8.3:-43031033 MS.gene25479:CDS
CGGAAGATACCGCAACATTA+TGG 0.304526 8.3:-43029961 MS.gene25479:CDS
GTTTCTAGTGGCTCTATTCC+TGG 0.309081 8.3:-43030071 MS.gene25479:CDS
AGATCGCCGCCAGAAACTTT+AGG 0.311063 8.3:+43030134 None:intergenic
TGAAGTCGATGTGTTAATTA+AGG 0.331599 8.3:-43028786 MS.gene25479:CDS
CGCCTTCTGGTCGCAATATT+CGG 0.333406 8.3:+43028825 None:intergenic
AATTGCTGTCAAACAGTATT+GGG 0.341304 8.3:-43028681 MS.gene25479:CDS
AACCAAGTGCTGCTGATAAT+AGG 0.347469 8.3:-43032394 MS.gene25479:CDS
CATATGTCAGGTTGCAAGTT+GGG 0.349075 8.3:-43031391 MS.gene25479:intron
ATTAATTCACTGTTGGATTC+CGG 0.352872 8.3:-43029981 MS.gene25479:CDS
AGAAGGCTGAAATTGCAATT+TGG 0.354585 8.3:-43030881 MS.gene25479:CDS
GTGCAGATCTTTATAGTAGT+TGG 0.359407 8.3:-43032359 MS.gene25479:CDS
TCGAAGGCTCTTGATGGATA+TGG 0.361140 8.3:+43031237 None:intergenic
TCTTCCTCAAGTTCCTCCTT+TGG 0.361668 8.3:+43030957 None:intergenic
GTTCTCGCTGTTTGATTCCA+TGG 0.364915 8.3:-43031203 MS.gene25479:CDS
ATTAAGGTAAAGAAGTTAAT+CGG 0.370727 8.3:-43028770 MS.gene25479:CDS
GCATGGCAAAGAAGTGGTTT+TGG 0.370846 8.3:-43032308 MS.gene25479:CDS
CGGATTATGACAGCGCCTTC+TGG 0.378229 8.3:+43028812 None:intergenic
AAAGGTGTTACATACCCTTT+TGG 0.379736 8.3:+43032008 None:intergenic
TAAACACATTTCCCTCGTAT+TGG 0.387315 8.3:+43031898 None:intergenic
TATGATCTCATTCATGCTAA+TGG 0.387963 8.3:-43029656 MS.gene25479:CDS
ATCTTAAACCATTTCCAAAC+AGG 0.389098 8.3:-43030115 MS.gene25479:CDS
ATCAGCACCTTGAGGAAATT+GGG 0.391471 8.3:+43031857 None:intergenic
TATGGATATGAATGCTGGTT+TGG 0.391975 8.3:-43029943 MS.gene25479:CDS
GGGACATAATCCCTACTCTT+AGG 0.394894 8.3:+43031978 None:intergenic
TCCTCCTTTGGTCTCTGCCA+TGG 0.397409 8.3:+43030969 None:intergenic
TTGAGAAGGCTATTCAGAAT+TGG 0.398684 8.3:-43031923 MS.gene25479:CDS
ACGGCACTCGACACCGGTTG+TGG 0.400333 8.3:-43031780 MS.gene25479:CDS
TAGTTGACCTTCCAATTGAT+TGG 0.405243 8.3:+43030996 None:intergenic
AATGAGATGGAATATGAAAT+TGG 0.407077 8.3:-43028744 MS.gene25479:CDS
TTCTCGCTGTTTGATTCCAT+GGG 0.411610 8.3:-43031202 MS.gene25479:CDS
GCATATAGCCTGTTTGGAAA+TGG 0.416366 8.3:+43030107 None:intergenic
AGGGGTGTACCTGCTGTTAT+TGG 0.418491 8.3:-43031282 MS.gene25479:CDS
TTGTAAGGTGCATTAGCATA+AGG 0.420580 8.3:+43031957 None:intergenic
GGGATCGCATATAGCCTGTT+TGG 0.420901 8.3:+43030101 None:intergenic
CATAATGTTGCGGTATCTTC+CGG 0.426759 8.3:+43029962 None:intergenic
CAAGAAAATTAATTCACTGT+TGG 0.428445 8.3:-43029988 MS.gene25479:CDS
ACAGCAATTAGTACTTTCTC+AGG 0.435226 8.3:+43028695 None:intergenic
AAACACCTTAGTGTTCGAAT+TGG 0.435639 8.3:+43032172 None:intergenic
CAATGTAACTTCTCTTCCTC+TGG 0.435812 8.3:+43032044 None:intergenic
AGGTTGCTAAGAAACTTTGC+TGG 0.437795 8.3:-43030917 MS.gene25479:CDS
TAATTGCTGTCAAACAGTAT+TGG 0.437862 8.3:-43028682 MS.gene25479:CDS
ATCTTGACACGGAGTGTAAT+CGG 0.437871 8.3:+43032127 None:intergenic
TGAATGAGATCATATGTTCT+TGG 0.437879 8.3:+43029665 None:intergenic
ATGGATATGAATGCTGGTTT+GGG 0.443758 8.3:-43029942 MS.gene25479:CDS
ATTTGGAACTACATCACATT+CGG 0.450872 8.3:+43032244 None:intergenic
TTCTTACCATTTGCTCCCCA+TGG 0.455409 8.3:+43031186 None:intergenic
TTGTCTGCTACGACATGATT+CGG 0.455765 8.3:+43030842 None:intergenic
CTCACTGTCCCATCATTGAT+AGG 0.457820 8.3:+43031804 None:intergenic
GCCATGTCGAAGGCTCTTGA+TGG 0.458291 8.3:+43031231 None:intergenic
TGATTGGCATCTATCATGAT+TGG 0.458687 8.3:-43029818 MS.gene25479:intron
TGGAAGTTGATAGAGTTCTA+AGG 0.464058 8.3:-43031049 MS.gene25479:CDS
AACATTATGGATATGAATGC+TGG 0.465021 8.3:-43029948 MS.gene25479:CDS
TTGATTCCATGGGGAGCAAA+TGG 0.467921 8.3:-43031192 MS.gene25479:intron
TCTGTTATTCCTATCAATGA+TGG 0.468006 8.3:-43031813 MS.gene25479:CDS
TTTCTGGTCCTCCAATCAAT+TGG 0.468116 8.3:-43031007 MS.gene25479:CDS
TGTATGATCCCAGCACCAAA+AGG 0.471853 8.3:-43032023 MS.gene25479:CDS
CCTCAGATCCTGATGATGTC+TGG 0.472305 8.3:-43030791 MS.gene25479:intron
AGTACAATTTGCTCTTGAAA+GGG 0.473622 8.3:-43031301 MS.gene25479:CDS
CATGCATACCAGACATCATC+AGG 0.476480 8.3:+43030783 None:intergenic
GGAAATTGGGTTCCACCACC+AGG 0.486235 8.3:+43031870 None:intergenic
ACATAAACATACCCCACAAC+CGG 0.490951 8.3:+43031767 None:intergenic
AAAAGGAGATAGTATAGCAT+TGG 0.491210 8.3:-43032285 MS.gene25479:CDS
AAGTACAATTTGCTCTTGAA+AGG 0.491607 8.3:-43031302 MS.gene25479:CDS
TGTAAGGTGCATTAGCATAA+GGG 0.498151 8.3:+43031958 None:intergenic
GGAAATGTGTTTAGATTCCC+TGG 0.500897 8.3:-43031888 MS.gene25479:CDS
TGCCATGTCATTTGCACCGA+GGG 0.503751 8.3:-43031340 MS.gene25479:CDS
ATATATGAGCGAGGATTGAT+TGG 0.505447 8.3:-43029834 MS.gene25479:CDS
GTCCTATTATCAGCAGCACT+TGG 0.509665 8.3:+43032392 None:intergenic
CGGCACTCGACACCGGTTGT+GGG 0.509777 8.3:-43031779 MS.gene25479:CDS
TCTTCTATATATTGGGAGCA+TGG 0.513373 8.3:-43032325 MS.gene25479:CDS
TGGCCTCTTTAGTTTGTACC+AGG 0.518458 8.3:-43029636 MS.gene25479:CDS
GTTATCATCCTGGTATGTCT+CGG 0.523970 8.3:+43030040 None:intergenic
CTACTCTTAGGCCATGGAAA+AGG 0.529226 8.3:+43031990 None:intergenic
TTGACCTTCCAATTGATTGG+AGG 0.532026 8.3:+43030999 None:intergenic
AGTTTCTTCCGAGACATACC+AGG 0.532827 8.3:-43030048 MS.gene25479:CDS
CTATTCATTCATCTAAGTCA+TGG 0.534410 8.3:-43029905 MS.gene25479:CDS
TATCCTGGTACAAACTAAAG+AGG 0.534977 8.3:+43029633 None:intergenic
ATCATATGTTCTTGGATATG+TGG 0.538349 8.3:+43029673 None:intergenic
TATGTCTCGGAAGAAACTCC+AGG 0.538435 8.3:+43030053 None:intergenic
TCATATGTCAGGTTGCAAGT+TGG 0.542053 8.3:-43031392 MS.gene25479:intron
CAAGAGTCTCACAGTTGAGA+AGG 0.546705 8.3:-43031937 MS.gene25479:CDS
GTGTTTAGATTCCCTGGTGG+TGG 0.546867 8.3:-43031882 MS.gene25479:CDS
GAAGGAACACTTACTTATCC+TGG 0.549922 8.3:+43029618 None:intergenic
ATATGTCAGGTTGCAAGTTG+GGG 0.550068 8.3:-43031390 MS.gene25479:intron
AAACAGTATTGGGTTACTGA+TGG 0.550680 8.3:-43028671 MS.gene25479:CDS
TCAACTGTGAGACTCTTGTA+AGG 0.555125 8.3:+43031942 None:intergenic
GGCACTCGACACCGGTTGTG+GGG 0.556598 8.3:-43031778 MS.gene25479:intron
GTTGATAGAGTTCTAAGGCC+TGG 0.557635 8.3:-43031044 MS.gene25479:CDS
CTGTTATTCCTATCAATGAT+GGG 0.558379 8.3:-43031812 MS.gene25479:CDS
TATTCATTCATCTAAGTCAT+GGG 0.558548 8.3:-43029904 MS.gene25479:CDS
TGAATCCAATTCGAACACTA+AGG 0.559388 8.3:-43032177 MS.gene25479:CDS
ATAGAGCCACTAGAAACTCT+AGG 0.562772 8.3:+43030077 None:intergenic
CAGTGATTATTGTGTTGATG+TGG 0.563280 8.3:+43028643 None:intergenic
AGAACAAAGAAAGATTGAAG+AGG 0.565209 8.3:-43030937 MS.gene25479:CDS
TGGTCCTCCAATCAATTGGA+AGG 0.566122 8.3:-43031003 MS.gene25479:CDS
AGTTAATCGGAGGAATGAGA+TGG 0.569106 8.3:-43028757 MS.gene25479:CDS
TGTGCTTCGTGAGAGTCCCT+CGG 0.570141 8.3:+43031324 None:intergenic
TAATCCCTACTCTTAGGCCA+TGG 0.572458 8.3:+43031984 None:intergenic
GGTTGCTAAGAAACTTTGCT+GGG 0.577899 8.3:-43030916 MS.gene25479:CDS
GAGACCAAAGGAGGAACTTG+AGG 0.584022 8.3:-43030961 MS.gene25479:CDS
GTGAGGACGGCACTCGACAC+CGG 0.585130 8.3:-43031786 MS.gene25479:CDS
TATGAAATTGGTCGATCATG+AGG 0.585763 8.3:-43028732 MS.gene25479:CDS
CCAGACATCATCAGGATCTG+AGG 0.586281 8.3:+43030791 None:intergenic
CTGGGAGAAGAAGTCTGAGA+AGG 0.587700 8.3:-43030898 MS.gene25479:CDS
AGAAGACATTCTTCTCGAGA+TGG 0.592742 8.3:-43028849 MS.gene25479:CDS
AGTACTTTCTCAGGAACAAG+AGG 0.593177 8.3:+43028704 None:intergenic
CTATAGGTATAAGAAAATGA+AGG 0.595392 8.3:-43030174 MS.gene25479:intron
GTATGATCCCAGCACCAAAA+GGG 0.596741 8.3:-43032022 MS.gene25479:CDS
TTGGGAGCATGGCAAAGAAG+TGG 0.598147 8.3:-43032314 MS.gene25479:CDS
ACCAGGATGATAACAAAATG+TGG 0.598954 8.3:-43030031 MS.gene25479:CDS
GTCCCTCGGTGCAAATGACA+TGG 0.599694 8.3:+43031338 None:intergenic
GACCGAATATTGCGACCAGA+AGG 0.601380 8.3:-43028827 MS.gene25479:CDS
CCGCCAGAAACTTTAGGAGT+CGG 0.604385 8.3:+43030140 None:intergenic
TGCAGATGGAATGTATATGA+TGG 0.612639 8.3:-43031069 MS.gene25479:intron
CAATGATGGGACAGTGAGGA+CGG 0.612966 8.3:-43031799 MS.gene25479:CDS
GTACAATTTGCTCTTGAAAG+GGG 0.614072 8.3:-43031300 MS.gene25479:CDS
TTTGACTAACTTCACCAGCA+TGG 0.614335 8.3:+43032204 None:intergenic
AAATTGGTCGATCATGAGGA+TGG 0.621937 8.3:-43028728 MS.gene25479:CDS
CATTCATATCCATAATGTTG+CGG 0.623801 8.3:+43029952 None:intergenic
ATAGCTGAGAAAAGTACGCT+CGG 0.625053 8.3:-43029861 MS.gene25479:CDS
AGCCACTAGAAACTCTAGGA+GGG 0.628438 8.3:+43030081 None:intergenic
CAGAATTGGATCCAATACGA+GGG 0.635879 8.3:-43031909 MS.gene25479:CDS
GGATTCGCAAAATTCTACAC+TGG 0.639914 8.3:+43030812 None:intergenic
ACACTCCGTGTCAAGATCAA+CGG 0.641064 8.3:-43032121 MS.gene25479:CDS
AGAACAATGAGCCATGTCGA+AGG 0.642478 8.3:+43031221 None:intergenic
AGAGAGACACTGTCCTCCAG+AGG 0.644444 8.3:-43032060 MS.gene25479:CDS
GGTGGAACCCAATTTCCTCA+AGG 0.645103 8.3:-43031864 MS.gene25479:CDS
GAGCCACTAGAAACTCTAGG+AGG 0.645440 8.3:+43030080 None:intergenic
GAAAGCACCCAATAACCACC+AGG 0.649776 8.3:+43031026 None:intergenic
GGAAGGTCAACTACAAACCA+TGG 0.650669 8.3:-43030986 MS.gene25479:CDS
TCCATCAAGAGCCTTCGACA+TGG 0.657437 8.3:-43031232 MS.gene25479:CDS
TCAGAATTGGATCCAATACG+AGG 0.658785 8.3:-43031910 MS.gene25479:CDS
ATCCCTCCTAGAGTTTCTAG+TGG 0.659300 8.3:-43030083 MS.gene25479:CDS
ATATATTTATCAGCACCTTG+AGG 0.663185 8.3:+43031849 None:intergenic
TGTAACTTCTCTTCCTCTGG+AGG 0.663464 8.3:+43032047 None:intergenic
AAGGTAAAGAAGTTAATCGG+AGG 0.666069 8.3:-43028767 MS.gene25479:CDS
CAAACCATGGCAGAGACCAA+AGG 0.668045 8.3:-43030973 MS.gene25479:CDS
CCGACTCCTAAAGTTTCTGG+CGG 0.668444 8.3:-43030140 MS.gene25479:CDS
GCTACGACATGATTCGGTGT+CGG 0.670657 8.3:+43030848 None:intergenic
TCTCGCTGTTTGATTCCATG+GGG 0.671713 8.3:-43031201 MS.gene25479:CDS
GAAATTGGGTTCCACCACCA+GGG 0.677362 8.3:+43031871 None:intergenic
ACCATGGCAGAGACCAAAGG+AGG 0.677739 8.3:-43030970 MS.gene25479:CDS
TCGGTATAACGAGCATCACA+AGG 0.677771 8.3:+43032146 None:intergenic
GATAGAGTTCTAAGGCCTGG+TGG 0.678122 8.3:-43031041 MS.gene25479:CDS
TTGCCATGTCATTTGCACCG+AGG 0.679888 8.3:-43031341 MS.gene25479:CDS
TGACTTAGATGAATGAATAG+CGG 0.692625 8.3:+43029908 None:intergenic
CTCGGTGTGATATATGAGCG+AGG 0.696906 8.3:-43029843 MS.gene25479:CDS
CTATCAATGATGGGACAGTG+AGG 0.728235 8.3:-43031803 MS.gene25479:CDS
AATGTGTTTAGATTCCCTGG+TGG 0.739635 8.3:-43031885 MS.gene25479:CDS
GCACGCCGTTGATCTTGACA+CGG 0.740888 8.3:+43032116 None:intergenic
TTAACACATCGACTTCATCG+CGG 0.761114 8.3:+43028792 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAGAAAAATGTATAAATAAA+GGG + chr8.3:43030694-43030713 None:intergenic 10.0%
!! ATAAATATATAACAACAAAA+AGG + chr8.3:43030301-43030320 None:intergenic 10.0%
!! TAAATATATAACAACAAAAA+GGG + chr8.3:43030300-43030319 None:intergenic 10.0%
!! TAAGAAAAATGTATAAATAA+AGG + chr8.3:43030695-43030714 None:intergenic 10.0%
!!! CACATTTTAATAAAAAAATT+AGG - chr8.3:43031765-43031784 MS.gene25479:intron 10.0%
!! AAAATTGTGTCAATTAAATT+GGG - chr8.3:43030730-43030749 MS.gene25479:intron 15.0%
!! AGAAAAATGTATAAATAAAG+GGG + chr8.3:43030693-43030712 None:intergenic 15.0%
!! ATTTAGTGTTATATTGTATT+GGG + chr8.3:43030629-43030648 None:intergenic 15.0%
!! TACATATTACTTTGATTATT+CGG - chr8.3:43029605-43029624 MS.gene25479:intron 15.0%
!! TATTTAGTGTTATATTGTAT+TGG + chr8.3:43030630-43030649 None:intergenic 15.0%
!!! GATTTTAATATCCATTTTAT+TGG + chr8.3:43031936-43031955 None:intergenic 15.0%
!!! TTATCTTTATTGTTTTATGT+TGG + chr8.3:43031886-43031905 None:intergenic 15.0%
!! AAAACAACATATAGCTTATA+TGG + chr8.3:43029439-43029458 None:intergenic 20.0%
!! AAAGTGGAATATATTGTATT+GGG + chr8.3:43030670-43030689 None:intergenic 20.0%
!! AACATACAAGAAAAAAAAAC+AGG + chr8.3:43031322-43031341 None:intergenic 20.0%
!! AAGTCATACAATCAATAATA+AGG - chr8.3:43031972-43031991 MS.gene25479:CDS 20.0%
!! ACAGCTTAATTAATTACTTA+TGG - chr8.3:43029366-43029385 MS.gene25479:intron 20.0%
!! AGTTGTATTGAAAATTGAAA+TGG + chr8.3:43030597-43030616 None:intergenic 20.0%
!! ATTAAGGTAAAGAAGTTAAT+CGG - chr8.3:43032280-43032299 MS.gene25479:CDS 20.0%
!! CAAAATTGTGTCAATTAAAT+TGG - chr8.3:43030729-43030748 MS.gene25479:intron 20.0%
!! GTTGTATTGAAAATTGAAAT+GGG + chr8.3:43030596-43030615 None:intergenic 20.0%
!! TAATGTTGTATAGAAATAAG+AGG - chr8.3:43030822-43030841 MS.gene25479:CDS 20.0%
!!! ACCATTTGACATTATAATTT+TGG - chr8.3:43029949-43029968 MS.gene25479:CDS 20.0%
!!! CACATTTTTTTCTTCAATTT+TGG - chr8.3:43031797-43031816 MS.gene25479:CDS 20.0%
!!! GACACTTTTTTTTAGAAAAT+GGG + chr8.3:43030560-43030579 None:intergenic 20.0%
!!! TTTTATAATGGCCATATTTA+GGG + chr8.3:43031645-43031664 None:intergenic 20.0%
!!! TTTTGTTGATTTTTTTATCC+AGG - chr8.3:43031341-43031360 MS.gene25479:CDS 20.0%
!!! TTTTTATAATGGCCATATTT+AGG + chr8.3:43031646-43031665 None:intergenic 20.0%
! AAAACTATTAGCTGCAAATA+AGG - chr8.3:43029922-43029941 MS.gene25479:CDS 25.0%
! AATGAGATGGAATATGAAAT+TGG - chr8.3:43032306-43032325 MS.gene25479:CDS 25.0%
! AATGTATGTATTCATATGTC+AGG - chr8.3:43029647-43029666 MS.gene25479:CDS 25.0%
! AGATCTGACATGAATAAAAT+AGG + chr8.3:43031280-43031299 None:intergenic 25.0%
! ATATATAACAACAAAAAGGG+AGG + chr8.3:43030297-43030316 None:intergenic 25.0%
! CAAGAAAATTAATTCACTGT+TGG - chr8.3:43031062-43031081 MS.gene25479:CDS 25.0%
! CTATAGGTATAAGAAAATGA+AGG - chr8.3:43030876-43030895 MS.gene25479:CDS 25.0%
! GAAAGTGGAATATATTGTAT+TGG + chr8.3:43030671-43030690 None:intergenic 25.0%
! GCCAAAATTATAATGTCAAA+TGG + chr8.3:43029953-43029972 None:intergenic 25.0%
! TAAAATAGGATCGAATAGTT+AGG + chr8.3:43031266-43031285 None:intergenic 25.0%
! TATATAACAACAAAAAGGGA+GGG + chr8.3:43030296-43030315 None:intergenic 25.0%
! TATTCATTCATCTAAGTCAT+GGG - chr8.3:43031146-43031165 MS.gene25479:intron 25.0%
! TTGGGAACTTAAAAAATAAG+CGG - chr8.3:43029471-43029490 MS.gene25479:intron 25.0%
!! AAAAACAAGTTTTCTGCTAA+TGG + chr8.3:43032100-43032119 None:intergenic 25.0%
!! ATTGTTCTAAATTGTGATTG+CGG - chr8.3:43030385-43030404 MS.gene25479:intron 25.0%
!! TTTTTAACCATGTAACAGTT+TGG + chr8.3:43031585-43031604 None:intergenic 25.0%
!!! CTCATTTTTCTGCAATATAA+AGG + chr8.3:43030422-43030441 None:intergenic 25.0%
!!! GGACACTTTTTTTTAGAAAA+TGG + chr8.3:43030561-43030580 None:intergenic 25.0%
!!! TGCTGTTTCTTCTATATATT+GGG - chr8.3:43028718-43028737 MS.gene25479:CDS 25.0%
AAAACAGGATCGAATAGTTA+AGG + chr8.3:43031307-43031326 None:intergenic 30.0%
AAAAGGAGATAGTATAGCAT+TGG - chr8.3:43028765-43028784 MS.gene25479:CDS 30.0%
AACATTATGGATATGAATGC+TGG - chr8.3:43031102-43031121 MS.gene25479:intron 30.0%
AATAAGCGGAAAACAACTTA+CGG - chr8.3:43029485-43029504 MS.gene25479:intron 30.0%
AATTGCTGTCAAACAGTATT+GGG - chr8.3:43032369-43032388 MS.gene25479:CDS 30.0%
AGAACAAAGAAAGATTGAAG+AGG - chr8.3:43030113-43030132 MS.gene25479:CDS 30.0%
ATATATTTATCAGCACCTTG+AGG + chr8.3:43029204-43029223 None:intergenic 30.0%
ATCATATGTTCTTGGATATG+TGG + chr8.3:43031380-43031399 None:intergenic 30.0%
ATCTTAAACCATTTCCAAAC+AGG - chr8.3:43030935-43030954 MS.gene25479:CDS 30.0%
ATTAATTCACTGTTGGATTC+CGG - chr8.3:43031069-43031088 MS.gene25479:intron 30.0%
CATTCATATCCATAATGTTG+CGG + chr8.3:43031101-43031120 None:intergenic 30.0%
CTATTCATTCATCTAAGTCA+TGG - chr8.3:43031145-43031164 MS.gene25479:intron 30.0%
CTGTTATTCCTATCAATGAT+GGG - chr8.3:43029238-43029257 MS.gene25479:intron 30.0%
TAATTGCTGTCAAACAGTAT+TGG - chr8.3:43032368-43032387 MS.gene25479:CDS 30.0%
TATGATCTCATTCATGCTAA+TGG - chr8.3:43031394-43031413 MS.gene25479:intron 30.0%
TCTGTTATTCCTATCAATGA+TGG - chr8.3:43029237-43029256 MS.gene25479:intron 30.0%
TGAAGTCGATGTGTTAATTA+AGG - chr8.3:43032264-43032283 MS.gene25479:CDS 30.0%
TGAATGAGATCATATGTTCT+TGG + chr8.3:43031388-43031407 None:intergenic 30.0%
TGACTTAGATGAATGAATAG+CGG + chr8.3:43031145-43031164 None:intergenic 30.0%
TGCGAGTCAAAACTTAAATT+TGG + chr8.3:43028826-43028845 None:intergenic 30.0%
TGTATAAATAAAGGGGAAAG+TGG + chr8.3:43030686-43030705 None:intergenic 30.0%
TTAAATAGTCATTTGACCGA+AGG + chr8.3:43031453-43031472 None:intergenic 30.0%
TTCTTTCGAAGCCAATAAAA+TGG - chr8.3:43031922-43031941 MS.gene25479:CDS 30.0%
TTGTGTCAATTAAATTGGGA+CGG - chr8.3:43030734-43030753 MS.gene25479:intron 30.0%
! AAGTACAATTTGCTCTTGAA+AGG - chr8.3:43029748-43029767 MS.gene25479:intron 30.0%
! AGTACAATTTGCTCTTGAAA+GGG - chr8.3:43029749-43029768 MS.gene25479:intron 30.0%
! ATTTGGAACTACATCACATT+CGG + chr8.3:43028809-43028828 None:intergenic 30.0%
! CTATAGTTCATGTTTTCTCT+CGG + chr8.3:43028970-43028989 None:intergenic 30.0%
! TGCAGCTAATAGTTTTTACA+TGG + chr8.3:43029918-43029937 None:intergenic 30.0%
! TTGAAATGGGTCAGTTATTT+TGG + chr8.3:43030583-43030602 None:intergenic 30.0%
!! AGATATTGACACTTGTTGTA+TGG - chr8.3:43030508-43030527 MS.gene25479:intron 30.0%
!! GTGCTGTTTCTTCTATATAT+TGG - chr8.3:43028717-43028736 MS.gene25479:CDS 30.0%
!! TGAAATGGGTCAGTTATTTT+GGG + chr8.3:43030582-43030601 None:intergenic 30.0%
!! TGTAGGTTTTAACGTAAGAT+GGG + chr8.3:43031720-43031739 None:intergenic 30.0%
!! TGTTTTTATAAGCTGTCGTT+GGG - chr8.3:43029453-43029472 MS.gene25479:intron 30.0%
!!! ATTTTTTTATCCAGGTGTGA+AGG - chr8.3:43031349-43031368 MS.gene25479:CDS 30.0%
!!! CTTGAAATGCTTTTGTTTTG+TGG - chr8.3:43030853-43030872 MS.gene25479:CDS 30.0%
!!! TCAGTTTTGTTAAACCTCAT+TGG - chr8.3:43029329-43029348 MS.gene25479:intron 30.0%
!!! TTGTTTTTATAAGCTGTCGT+TGG - chr8.3:43029452-43029471 MS.gene25479:intron 30.0%
AAACACCTTAGTGTTCGAAT+TGG + chr8.3:43028881-43028900 None:intergenic 35.0%
AAATTAGCCACAATCCAAAC+TGG - chr8.3:43029578-43029597 MS.gene25479:intron 35.0%
AAGGTAAAGAAGTTAATCGG+AGG - chr8.3:43032283-43032302 MS.gene25479:CDS 35.0%
ACACTCTTGTGAAACTGTTT+GGG + chr8.3:43029540-43029559 None:intergenic 35.0%
ACAGCAATTAGTACTTTCTC+AGG + chr8.3:43032358-43032377 None:intergenic 35.0%
ACCAGGATGATAACAAAATG+TGG - chr8.3:43031019-43031038 MS.gene25479:CDS 35.0%
AGAAAATGGGTCAGTTATTC+CGG + chr8.3:43030547-43030566 None:intergenic 35.0%
AGAAGGCTGAAATTGCAATT+TGG - chr8.3:43030169-43030188 MS.gene25479:CDS 35.0%
AGGATAAGTAAGTGTTCCTT+CGG - chr8.3:43031434-43031453 MS.gene25479:intron 35.0%
ATATATGAGCGAGGATTGAT+TGG - chr8.3:43031216-43031235 MS.gene25479:CDS 35.0%
GACAAGCAGAGAGAATTAAA+AGG + chr8.3:43030342-43030361 None:intergenic 35.0%
GAGGTTTAACAAAACTGATC+AGG + chr8.3:43029327-43029346 None:intergenic 35.0%
GCTAATTTGAGCTTATCTAC+TGG + chr8.3:43029566-43029585 None:intergenic 35.0%
GTAATATGTACAAGCCAGTT+TGG + chr8.3:43029595-43029614 None:intergenic 35.0%
GTGCAGATCTTTATAGTAGT+TGG - chr8.3:43028691-43028710 MS.gene25479:CDS 35.0%
TAAACACATTTCCCTCGTAT+TGG + chr8.3:43029155-43029174 None:intergenic 35.0%
TAAGCCAGTTCTTATTCCAT+AGG - chr8.3:43031496-43031515 MS.gene25479:intron 35.0%
TACACTCTTGTGAAACTGTT+TGG + chr8.3:43029541-43029560 None:intergenic 35.0%
TAGTTGACCTTCCAATTGAT+TGG + chr8.3:43030057-43030076 None:intergenic 35.0%
TATCCTGGTACAAACTAAAG+AGG + chr8.3:43031420-43031439 None:intergenic 35.0%
TATGAAATTGGTCGATCATG+AGG - chr8.3:43032318-43032337 MS.gene25479:CDS 35.0%
TCCATTTCAGTCCCTAAATA+TGG - chr8.3:43031631-43031650 MS.gene25479:intron 35.0%
TCTTCTATATATTGGGAGCA+TGG - chr8.3:43028725-43028744 MS.gene25479:CDS 35.0%
TGAATCCAATTCGAACACTA+AGG - chr8.3:43028873-43028892 MS.gene25479:intron 35.0%
TGATTGGCATCTATCATGAT+TGG - chr8.3:43031232-43031251 MS.gene25479:CDS 35.0%
TGCAGATGGAATGTATATGA+TGG - chr8.3:43029981-43030000 MS.gene25479:CDS 35.0%
TGTAAGGTGCATTAGCATAA+GGG + chr8.3:43029095-43029114 None:intergenic 35.0%
TTGAGAAGGCTATTCAGAAT+TGG - chr8.3:43029127-43029146 MS.gene25479:intron 35.0%
TTGTAAGGTGCATTAGCATA+AGG + chr8.3:43029096-43029115 None:intergenic 35.0%
! AAACAGTATTGGGTTACTGA+TGG - chr8.3:43032379-43032398 MS.gene25479:CDS 35.0%
! GATTCTTTTAGTCGTATGCT+CGG + chr8.3:43030466-43030485 None:intergenic 35.0%
! GTACAATTTGCTCTTGAAAG+GGG - chr8.3:43029750-43029769 MS.gene25479:intron 35.0%
! GTACTTTTCTCAGCTATAGT+AGG + chr8.3:43031186-43031205 None:intergenic 35.0%
! TATGGATATGAATGCTGGTT+TGG - chr8.3:43031107-43031126 MS.gene25479:intron 35.0%
! TGATGGATATGGCAACTTTA+TGG + chr8.3:43029805-43029824 None:intergenic 35.0%
!! ATGGATATGAATGCTGGTTT+GGG - chr8.3:43031108-43031127 MS.gene25479:intron 35.0%
!! GTGTAGGTTTTAACGTAAGA+TGG + chr8.3:43031721-43031740 None:intergenic 35.0%
!! TCCACATTTTGTTATCATCC+TGG + chr8.3:43031023-43031042 None:intergenic 35.0%
!! TGGAAGTTGATAGAGTTCTA+AGG - chr8.3:43030001-43030020 MS.gene25479:CDS 35.0%
!!! AAAGGTGTTACATACCCTTT+TGG + chr8.3:43029045-43029064 None:intergenic 35.0%
!!! CAAAGAAGTGGTTTTGGAAA+AGG - chr8.3:43028748-43028767 MS.gene25479:CDS 35.0%
!!! TGCTTTTGTTTTGTGGCTAT+AGG - chr8.3:43030860-43030879 MS.gene25479:CDS 35.0%
AAACTGTTTGGGAGAGCTTA+TGG + chr8.3:43029529-43029548 None:intergenic 40.0%
AAATTGGTCGATCATGAGGA+TGG - chr8.3:43032322-43032341 MS.gene25479:CDS 40.0%
AACTTCACCCAGTATTGCAA+TGG - chr8.3:43031530-43031549 MS.gene25479:intron 40.0%
AAGTTAGTAGTCTACGCCTA+TGG + chr8.3:43031515-43031534 None:intergenic 40.0%
AATGTGTTTAGATTCCCTGG+TGG - chr8.3:43029165-43029184 MS.gene25479:intron 40.0%
ACAGATCCCATTGCAATACT+GGG + chr8.3:43031540-43031559 None:intergenic 40.0%
ACATAAACATACCCCACAAC+CGG + chr8.3:43029286-43029305 None:intergenic 40.0%
ACTTCACCCAGTATTGCAAT+GGG - chr8.3:43031531-43031550 MS.gene25479:intron 40.0%
AGAAGACATTCTTCTCGAGA+TGG - chr8.3:43032201-43032220 MS.gene25479:CDS 40.0%
AGAGTAGCAAGTTTCTTCTG+GGG + chr8.3:43031692-43031711 None:intergenic 40.0%
AGGTTGCTAAGAAACTTTGC+TGG - chr8.3:43030133-43030152 MS.gene25479:CDS 40.0%
AGTACTTTCTCAGGAACAAG+AGG + chr8.3:43032349-43032368 None:intergenic 40.0%
AGTTAATCGGAGGAATGAGA+TGG - chr8.3:43032293-43032312 MS.gene25479:CDS 40.0%
ATAGAGCCACTAGAAACTCT+AGG + chr8.3:43030976-43030995 None:intergenic 40.0%
ATCAGCACCTTGAGGAAATT+GGG + chr8.3:43029196-43029215 None:intergenic 40.0%
ATCTTGACACGGAGTGTAAT+CGG + chr8.3:43028926-43028945 None:intergenic 40.0%
CAATGTAACTTCTCTTCCTC+TGG + chr8.3:43029009-43029028 None:intergenic 40.0%
CAGAATTGGATCCAATACGA+GGG - chr8.3:43029141-43029160 MS.gene25479:intron 40.0%
CATAATGTTGCGGTATCTTC+CGG + chr8.3:43031091-43031110 None:intergenic 40.0%
CCAAAAACACCAATAACAGC+AGG + chr8.3:43029780-43029799 None:intergenic 40.0%
CTTATCATCGAGAGTAGTGT+AGG + chr8.3:43031737-43031756 None:intergenic 40.0%
GAAAATGGGTCAGTTATTCC+GGG + chr8.3:43030546-43030565 None:intergenic 40.0%
GAAGGAACACTTACTTATCC+TGG + chr8.3:43031435-43031454 None:intergenic 40.0%
GAGAGTAGCAAGTTTCTTCT+GGG + chr8.3:43031693-43031712 None:intergenic 40.0%
GCATATAGCCTGTTTGGAAA+TGG + chr8.3:43030946-43030965 None:intergenic 40.0%
GCCATATTTAGGGACTGAAA+TGG + chr8.3:43031635-43031654 None:intergenic 40.0%
GGAAATGTGTTTAGATTCCC+TGG - chr8.3:43029162-43029181 MS.gene25479:intron 40.0%
GGATTCGCAAAATTCTACAC+TGG + chr8.3:43030241-43030260 None:intergenic 40.0%
GGCATCTATCATGATTGGTA+TGG - chr8.3:43031237-43031256 MS.gene25479:CDS 40.0%
GGTTGCTAAGAAACTTTGCT+GGG - chr8.3:43030134-43030153 MS.gene25479:CDS 40.0%
GTTATCATCCTGGTATGTCT+CGG + chr8.3:43031013-43031032 None:intergenic 40.0%
TACAGATCCCATTGCAATAC+TGG + chr8.3:43031541-43031560 None:intergenic 40.0%
TACGCCTATGGAATAAGAAC+TGG + chr8.3:43031503-43031522 None:intergenic 40.0%
TATCAGCACCTTGAGGAAAT+TGG + chr8.3:43029197-43029216 None:intergenic 40.0%
TCAACTGTGAGACTCTTGTA+AGG + chr8.3:43029111-43029130 None:intergenic 40.0%
TCAGAATTGGATCCAATACG+AGG - chr8.3:43029140-43029159 MS.gene25479:intron 40.0%
TGAGTCTCCAAACTGTTACA+TGG - chr8.3:43031575-43031594 MS.gene25479:intron 40.0%
TGTCAATTAAATTGGGACGG+AGG - chr8.3:43030737-43030756 MS.gene25479:intron 40.0%
TTCTCGCTGTTTGATTCCAT+GGG - chr8.3:43029848-43029867 MS.gene25479:CDS 40.0%
TTGACCTTCCAATTGATTGG+AGG + chr8.3:43030054-43030073 None:intergenic 40.0%
TTGTCTGCTACGACATGATT+CGG + chr8.3:43030211-43030230 None:intergenic 40.0%
TTTGACTAACTTCACCAGCA+TGG + chr8.3:43028849-43028868 None:intergenic 40.0%
! AACCAAGTGCTGCTGATAAT+AGG - chr8.3:43028656-43028675 MS.gene25479:CDS 40.0%
! ATAGCTGAGAAAAGTACGCT+CGG - chr8.3:43031189-43031208 MS.gene25479:intron 40.0%
! ATATGTCAGGTTGCAAGTTG+GGG - chr8.3:43029660-43029679 MS.gene25479:CDS 40.0%
! CATATGTCAGGTTGCAAGTT+GGG - chr8.3:43029659-43029678 MS.gene25479:CDS 40.0%
! GGTATGTAACACCTTTTCCA+TGG - chr8.3:43029049-43029068 MS.gene25479:intron 40.0%
! TCATATGTCAGGTTGCAAGT+TGG - chr8.3:43029658-43029677 MS.gene25479:CDS 40.0%
! TTTCTGGTCCTCCAATCAAT+TGG - chr8.3:43030043-43030062 MS.gene25479:CDS 40.0%
! TTTTCCATGGCCTAAGAGTA+GGG - chr8.3:43029062-43029081 MS.gene25479:intron 40.0%
!! AAGCACTTTTTACCTGTGCT+TGG - chr8.3:43031603-43031622 MS.gene25479:intron 40.0%
!! AGCACTTTTTACCTGTGCTT+GGG - chr8.3:43031604-43031623 MS.gene25479:intron 40.0%
!! AGGTTTTAACGTAAGATGGG+AGG + chr8.3:43031717-43031736 None:intergenic 40.0%
!! CCTGCTGTTATTGGTGTTTT+TGG - chr8.3:43029777-43029796 MS.gene25479:intron 40.0%
!! GGTTTTAACGTAAGATGGGA+GGG + chr8.3:43031716-43031735 None:intergenic 40.0%
!! GTTTTAACGTAAGATGGGAG+GGG + chr8.3:43031715-43031734 None:intergenic 40.0%
!! TTAACACATCGACTTCATCG+CGG + chr8.3:43032261-43032280 None:intergenic 40.0%
!! TTTGGCTGTTGATTTGCAGA+TGG - chr8.3:43029967-43029986 MS.gene25479:CDS 40.0%
!!! TTACATACCCTTTTGGTGCT+GGG + chr8.3:43029038-43029057 None:intergenic 40.0%
ACACTCCGTGTCAAGATCAA+CGG - chr8.3:43028929-43028948 MS.gene25479:intron 45.0%
ACTCCGACTCCTAAAGTTTC+TGG - chr8.3:43030907-43030926 MS.gene25479:CDS 45.0%
AGAACAATGAGCCATGTCGA+AGG + chr8.3:43029832-43029851 None:intergenic 45.0%
AGTTTCTTCCGAGACATACC+AGG - chr8.3:43031002-43031021 MS.gene25479:CDS 45.0%
CAAGAGTCTCACAGTTGAGA+AGG - chr8.3:43029113-43029132 MS.gene25479:intron 45.0%
CATGCATACCAGACATCATC+AGG + chr8.3:43030270-43030289 None:intergenic 45.0%
CGGAAGATACCGCAACATTA+TGG - chr8.3:43031089-43031108 MS.gene25479:intron 45.0%
CTACTCTTAGGCCATGGAAA+AGG + chr8.3:43029063-43029082 None:intergenic 45.0%
CTCACTGTCCCATCATTGAT+AGG + chr8.3:43029249-43029268 None:intergenic 45.0%
GGAAGGTCAACTACAAACCA+TGG - chr8.3:43030064-43030083 MS.gene25479:CDS 45.0%
GGAGAGTAGCAAGTTTCTTC+TGG + chr8.3:43031694-43031713 None:intergenic 45.0%
GGGACATAATCCCTACTCTT+AGG + chr8.3:43029075-43029094 None:intergenic 45.0%
GTACAAGCCAGTTTGGATTG+TGG + chr8.3:43029588-43029607 None:intergenic 45.0%
GTATGATCCCAGCACCAAAA+GGG - chr8.3:43029028-43029047 MS.gene25479:intron 45.0%
GTCCTATTATCAGCAGCACT+TGG + chr8.3:43028661-43028680 None:intergenic 45.0%
GTTCTCGCTGTTTGATTCCA+TGG - chr8.3:43029847-43029866 MS.gene25479:CDS 45.0%
TAAACCTCATTGGCGCTGTT+TGG - chr8.3:43029339-43029358 MS.gene25479:intron 45.0%
TAATCCCTACTCTTAGGCCA+TGG + chr8.3:43029069-43029088 None:intergenic 45.0%
TATGTCTCGGAAGAAACTCC+AGG + chr8.3:43031000-43031019 None:intergenic 45.0%
TCGGTATAACGAGCATCACA+AGG + chr8.3:43028907-43028926 None:intergenic 45.0%
TCTCGCTGTTTGATTCCATG+GGG - chr8.3:43029849-43029868 MS.gene25479:CDS 45.0%
TCTTCCTCAAGTTCCTCCTT+TGG + chr8.3:43030096-43030115 None:intergenic 45.0%
TGGTCCTCCAATCAATTGGA+AGG - chr8.3:43030047-43030066 MS.gene25479:CDS 45.0%
TGTAACTTCTCTTCCTCTGG+AGG + chr8.3:43029006-43029025 None:intergenic 45.0%
TGTATGATCCCAGCACCAAA+AGG - chr8.3:43029027-43029046 MS.gene25479:intron 45.0%
TTAACCAAACAGCGCCAATG+AGG + chr8.3:43029346-43029365 None:intergenic 45.0%
TTCTTACCATTTGCTCCCCA+TGG + chr8.3:43029867-43029886 None:intergenic 45.0%
TTGATTCCATGGGGAGCAAA+TGG - chr8.3:43029858-43029877 MS.gene25479:CDS 45.0%
! AGCCACTAGAAACTCTAGGA+GGG + chr8.3:43030972-43030991 None:intergenic 45.0%
! ATCCCTCCTAGAGTTTCTAG+TGG - chr8.3:43030967-43030986 MS.gene25479:CDS 45.0%
! CAAGTTGGGGTGCATATCTT+TGG - chr8.3:43029673-43029692 MS.gene25479:CDS 45.0%
! CTATCAATGATGGGACAGTG+AGG - chr8.3:43029247-43029266 MS.gene25479:intron 45.0%
! CTTTTCCATGGCCTAAGAGT+AGG - chr8.3:43029061-43029080 MS.gene25479:intron 45.0%
! GCATGGCAAAGAAGTGGTTT+TGG - chr8.3:43028742-43028761 MS.gene25479:CDS 45.0%
! TCGAAGGCTCTTGATGGATA+TGG + chr8.3:43029816-43029835 None:intergenic 45.0%
! TCTAAGGCCTGGTGGTTATT+GGG - chr8.3:43030017-43030036 MS.gene25479:CDS 45.0%
!! GTTACATACCCTTTTGGTGC+TGG + chr8.3:43029039-43029058 None:intergenic 45.0%
!! GTTGATAGAGTTCTAAGGCC+TGG - chr8.3:43030006-43030025 MS.gene25479:CDS 45.0%
!! GTTTCTAGTGGCTCTATTCC+TGG - chr8.3:43030979-43030998 MS.gene25479:CDS 45.0%
!! TGGCCTCTTTAGTTTGTACC+AGG - chr8.3:43031414-43031433 MS.gene25479:intron 45.0%
!! TTCTAAGGCCTGGTGGTTAT+TGG - chr8.3:43030016-43030035 MS.gene25479:CDS 45.0%
!!! TTTTTTCATATTTTTTATAA+TGG + chr8.3:43031657-43031676 None:intergenic 5.0%
AAATGGACACACCCAAGCAC+AGG + chr8.3:43031618-43031637 None:intergenic 50.0%
AGATCGCCGCCAGAAACTTT+AGG + chr8.3:43030919-43030938 None:intergenic 50.0%
ATGGGTCAGTTATTCCGGGA+CGG + chr8.3:43030542-43030561 None:intergenic 50.0%
CAAACCATGGCAGAGACCAA+AGG - chr8.3:43030077-43030096 MS.gene25479:CDS 50.0%
CCAGACATCATCAGGATCTG+AGG + chr8.3:43030262-43030281 None:intergenic 50.0%
CGCCTTCTGGTCGCAATATT+CGG + chr8.3:43032228-43032247 None:intergenic 50.0%
CTGGGAGAAGAAGTCTGAGA+AGG - chr8.3:43030152-43030171 MS.gene25479:CDS 50.0%
GAAATTGGGTTCCACCACCA+GGG + chr8.3:43029182-43029201 None:intergenic 50.0%
GACCGAATATTGCGACCAGA+AGG - chr8.3:43032223-43032242 MS.gene25479:CDS 50.0%
GAGACCAAAGGAGGAACTTG+AGG - chr8.3:43030089-43030108 MS.gene25479:CDS 50.0%
GAGCCACTAGAAACTCTAGG+AGG + chr8.3:43030973-43030992 None:intergenic 50.0%
GGGATCGCATATAGCCTGTT+TGG + chr8.3:43030952-43030971 None:intergenic 50.0%
GGTGGAACCCAATTTCCTCA+AGG - chr8.3:43029186-43029205 MS.gene25479:intron 50.0%
GGTGGTTATTGGGTGCTTTC+TGG - chr8.3:43030027-43030046 MS.gene25479:CDS 50.0%
GTGTTTAGATTCCCTGGTGG+TGG - chr8.3:43029168-43029187 MS.gene25479:intron 50.0%
TAGTCGTATGCTCGGATGCA+TGG + chr8.3:43030458-43030477 None:intergenic 50.0%
TCCATCAAGAGCCTTCGACA+TGG - chr8.3:43029818-43029837 MS.gene25479:intron 50.0%
TGTGGAAAAGCCTTCACACC+TGG + chr8.3:43031362-43031381 None:intergenic 50.0%
! AGGGGTGTACCTGCTGTTAT+TGG - chr8.3:43029768-43029787 MS.gene25479:intron 50.0%
! CAATGATGGGACAGTGAGGA+CGG - chr8.3:43029251-43029270 MS.gene25479:intron 50.0%
! CCGACTCCTAAAGTTTCTGG+CGG - chr8.3:43030910-43030929 MS.gene25479:CDS 50.0%
! CCGCCAGAAACTTTAGGAGT+CGG + chr8.3:43030913-43030932 None:intergenic 50.0%
! CCTCAGATCCTGATGATGTC+TGG - chr8.3:43030259-43030278 MS.gene25479:intron 50.0%
! CTCGGTGTGATATATGAGCG+AGG - chr8.3:43031207-43031226 MS.gene25479:CDS 50.0%
! TGCCATGTCATTTGCACCGA+GGG - chr8.3:43029710-43029729 MS.gene25479:intron 50.0%
! TTGCCATGTCATTTGCACCG+AGG - chr8.3:43029709-43029728 MS.gene25479:intron 50.0%
! TTGGGAGCATGGCAAAGAAG+TGG - chr8.3:43028736-43028755 MS.gene25479:CDS 50.0%
!! AGCAAGTTTCTTCTGGGGCA+GGG + chr8.3:43031687-43031706 None:intergenic 50.0%
!! GAAAGCACCCAATAACCACC+AGG + chr8.3:43030027-43030046 None:intergenic 50.0%
!! GATAGAGTTCTAAGGCCTGG+TGG - chr8.3:43030009-43030028 MS.gene25479:CDS 50.0%
!! GCTACGACATGATTCGGTGT+CGG + chr8.3:43030205-43030224 None:intergenic 50.0%
!! TAGCAAGTTTCTTCTGGGGC+AGG + chr8.3:43031688-43031707 None:intergenic 50.0%
!!! AGTTTTGACTCGCACCATGC+TGG - chr8.3:43028832-43028851 MS.gene25479:CDS 50.0%
ACCATGGCAGAGACCAAAGG+AGG - chr8.3:43030080-43030099 MS.gene25479:CDS 55.0%
AGAGAGACACTGTCCTCCAG+AGG - chr8.3:43028990-43029009 MS.gene25479:intron 55.0%
CGGATTATGACAGCGCCTTC+TGG + chr8.3:43032241-43032260 None:intergenic 55.0%
GCACGCCGTTGATCTTGACA+CGG + chr8.3:43028937-43028956 None:intergenic 55.0%
GCCATGTCGAAGGCTCTTGA+TGG + chr8.3:43029822-43029841 None:intergenic 55.0%
GGAAATTGGGTTCCACCACC+AGG + chr8.3:43029183-43029202 None:intergenic 55.0%
GTCAGTTATTCCGGGACGGA+GGG + chr8.3:43030538-43030557 None:intergenic 55.0%
TCCTCCTTTGGTCTCTGCCA+TGG + chr8.3:43030084-43030103 None:intergenic 55.0%
TGTGCTTCGTGAGAGTCCCT+CGG + chr8.3:43029729-43029748 None:intergenic 55.0%
! GTCCCTCGGTGCAAATGACA+TGG + chr8.3:43029715-43029734 None:intergenic 55.0%
GGTCAGTTATTCCGGGACGG+AGG + chr8.3:43030539-43030558 None:intergenic 60.0%
GTATGGTACTCCCTCCGTCC+CGG - chr8.3:43030525-43030544 MS.gene25479:intron 60.0%
GTGAGGACGGCACTCGACAC+CGG - chr8.3:43029264-43029283 MS.gene25479:intron 65.0%
! ACGGCACTCGACACCGGTTG+TGG - chr8.3:43029270-43029289 MS.gene25479:intron 65.0%
! CGGCACTCGACACCGGTTGT+GGG - chr8.3:43029271-43029290 MS.gene25479:intron 65.0%
! GGCACTCGACACCGGTTGTG+GGG - chr8.3:43029272-43029291 MS.gene25479:intron 65.0%


Chromosome Type Strat End Strand Name
chr8.3 gene 43028649 43032423 43028649 ID=MS.gene25479
chr8.3 mRNA 43028649 43032423 43028649 ID=MS.gene25479.t1;Parent=MS.gene25479
chr8.3 exon 43031779 43032423 43031779 ID=MS.gene25479.t1.exon1;Parent=MS.gene25479.t1
chr8.3 CDS 43031779 43032423 43031779 ID=cds.MS.gene25479.t1;Parent=MS.gene25479.t1
chr8.3 exon 43031193 43031403 43031193 ID=MS.gene25479.t1.exon2;Parent=MS.gene25479.t1
chr8.3 CDS 43031193 43031403 43031193 ID=cds.MS.gene25479.t1;Parent=MS.gene25479.t1
chr8.3 exon 43030792 43031086 43030792 ID=MS.gene25479.t1.exon3;Parent=MS.gene25479.t1
chr8.3 CDS 43030792 43031086 43030792 ID=cds.MS.gene25479.t1;Parent=MS.gene25479.t1
chr8.3 exon 43029819 43030190 43029819 ID=MS.gene25479.t1.exon4;Parent=MS.gene25479.t1
chr8.3 CDS 43029819 43030190 43029819 ID=cds.MS.gene25479.t1;Parent=MS.gene25479.t1
chr8.3 exon 43029632 43029709 43029632 ID=MS.gene25479.t1.exon5;Parent=MS.gene25479.t1
chr8.3 CDS 43029632 43029709 43029632 ID=cds.MS.gene25479.t1;Parent=MS.gene25479.t1
chr8.3 exon 43028649 43028880 43028649 ID=MS.gene25479.t1.exon6;Parent=MS.gene25479.t1
chr8.3 CDS 43028649 43028880 43028649 ID=cds.MS.gene25479.t1;Parent=MS.gene25479.t1
Gene Sequence

>MS.gene25479

ATGGCAAAACCAAGTGCTGCTGATAATAGGACTAGAAGTTCAGTGCAGATCTTTATAGTAGTTGGTTTGTGCTGTTTCTTCTATATATTGGGAGCATGGCAAAGAAGTGGTTTTGGAAAAGGAGATAGTATAGCATTGGAGATTACAAAGAATAACGCCGAATGTGATGTAGTTCCAAATTTAAGTTTTGACTCGCACCATGCTGGTGAAGTTAGTCAAATCGATGAATCCAATTCGAACACTAAGGTGTTTAAACCTTGTGATGCTCGTTATACCGATTACACTCCGTGTCAAGATCAACGGCGTGCTATGACGTTTCCGAGAGAAAACATGAACTATAGAGAGAGACACTGTCCTCCAGAGGAAGAGAAGTTACATTGTATGATCCCAGCACCAAAAGGGTATGTAACACCTTTTCCATGGCCTAAGAGTAGGGATTATGTCCCTTATGCTAATGCACCTTACAAGAGTCTCACAGTTGAGAAGGCTATTCAGAATTGGATCCAATACGAGGGAAATGTGTTTAGATTCCCTGGTGGTGGAACCCAATTTCCTCAAGGTGCTGATAAATATATTGATCAACTCGCATCTGTTATTCCTATCAATGATGGGACAGTGAGGACGGCACTCGACACCGGTTGTGGGGTATGTTTATGTTTTTATTTATCTCAAATTCCTGATCAGTTTTGTTAAACCTCATTGGCGCTGTTTGGTTAAACAGCTTAATTAATTACTTATGGTATAAGCTATTTCTATAACAAAGATAAAATATTGTCAAAGTGTTTTTCCATATAAGCTATATGTTGTTTTTATAAGCTGTCGTTGGGAACTTAAAAAATAAGCGGAAAACAACTTACGGATATGTCGTAAACTGTTTCCATAAGCTCTCCCAAACAGTTTCACAAGAGTGTATGCCAGTAGATAAGCTCAAATTAGCCACAATCCAAACTGGCTTGTACATATTACTTTGATTATTCGGTTCATTATTTGTATTAAGCAATGTATGTATTCATATGTCAGGTTGCAAGTTGGGGTGCATATCTTTGGAGCAGAAATGTTGTTGCCATGTCATTTGCACCGAGGGACTCTCACGAAGCACAAGTACAATTTGCTCTTGAAAGGGGTGTACCTGCTGTTATTGGTGTTTTTGGAACCATAAAGTTGCCATATCCATCAAGAGCCTTCGACATGGCTCATTGTTCTCGCTGTTTGATTCCATGGGGAGCAAATGGTAAGAACATCAAAGTTAAAGTAAACAATGTTTTACCATGTAAAAACTATTAGCTGCAAATAAGGAAGTACCATTTGACATTATAATTTTGGCTGTTGATTTGCAGATGGAATGTATATGATGGAAGTTGATAGAGTTCTAAGGCCTGGTGGTTATTGGGTGCTTTCTGGTCCTCCAATCAATTGGAAGGTCAACTACAAACCATGGCAGAGACCAAAGGAGGAACTTGAGGAAGAACAAAGAAAGATTGAAGAGGTTGCTAAGAAACTTTGCTGGGAGAAGAAGTCTGAGAAGGCTGAAATTGCAATTTGGCAAAAGATGACCGACACCGAATCATGTCGTAGCAGACAAGATGACTCCAGTGTAGAATTTTGCGAATCCTCAGATCCTGATGATGTCTGGTATGCATGCTTCCCTCCCTTTTTGTTGTTATATATTTATCATTTATTTGTTGACGTTCCTTTTAATTCTCTCTGCTTGTCATGAGAACATACATGTGTGTGATATTGTTCTAAATTGTGATTGCGGTTAAGCTCAAACCTTTATATTGCAGAAAAATGAGATCAATTTTAACACCATGCATCCGAGCATACGACTAAAAGAATCTGATATTGTCTAGTGATGTATTAGATATTGACACTTGTTGTATGGTACTCCCTCCGTCCCGGAATAACTGACCCATTTTCTAAAAAAAAGTGTCCCAAAATAACTGACCCATTTCAATTTTCAATACAACTTTCATCATTCCCAATACAATATAACACTAAATAATACTACTATTATCATTCCCAATACAATATATTCCACTTTCCCCTTTATTTATACATTTTTCTTAATATGTGTGAAAAACAAAATTGTGTCAATTAAATTGGGACGGAGGTAGTATATGTTATTGATATCGTCTTTATTGATTTTTTTAATTGTATAAATATGAGCAATGTTTAATGTTGTATAGAAATAAGAGGTGTACTGACTTGAAATGCTTTTGTTTTGTGGCTATAGGTATAAGAAAATGAAGGCTTGCGTTACTCCGACTCCTAAAGTTTCTGGCGGCGATCTTAAACCATTTCCAAACAGGCTATATGCGATCCCTCCTAGAGTTTCTAGTGGCTCTATTCCTGGAGTTTCTTCCGAGACATACCAGGATGATAACAAAATGTGGAAAAAGCATGTCAATGCATACAAGAAAATTAATTCACTGTTGGATTCCGGAAGATACCGCAACATTATGGATATGAATGCTGGTTTGGGTAGTTTTGCTGCCGCTATTCATTCATCTAAGTCATGGGTCATGAATGTTGTGCCTACTATAGCTGAGAAAAGTACGCTCGGTGTGATATATGAGCGAGGATTGATTGGCATCTATCATGATTGGTATGGTTTCCTAACTATTCGATCCTATTTTATTCATGTCAGATCTCAATCCTTAACTATTCGATCCTGTTTTTTTTTCTTGTATGTTTTTGTTGATTTTTTTATCCAGGTGTGAAGGCTTTTCCACATATCCAAGAACATATGATCTCATTCATGCTAATGGCCTCTTTAGTTTGTACCAGGATAAGTAAGTGTTCCTTCGGTCAAATGACTATTTAACTATTCGTTTTTGTTAAGTTTTATAAGCCAGTTCTTATTCCATAGGCGTAGACTACTAACTTCACCCAGTATTGCAATGGGATCTGTAGTAAGCTTGTTTGTTGAGTCTCCAAACTGTTACATGGTTAAAAAGCACTTTTTACCTGTGCTTGGGTGTGTCCATTTCAGTCCCTAAATATGGCCATTATAAAAAATATGAAAAAATTACACTCCCTGCCCCAGAAGAAACTTGCTACTCTCCCCTCCCATCTTACGTTAAAACCTACACTACTCTCGATGATAAGTGAATATGCACATTTTAATAAAAAAATTAGGATTCTTATGCACATTTTTTTCTTCAATTTTGGCTCTTATTATCTTTTTTTTTACATATAGTTTTGAAATCTTACTAGTATCAATGTTAATTTATACCAACATAAAACAATAAAGATAAACTAAAGTTATGAGCATTCTTTCGAAGCCAATAAAATGGATATTAAAATCTTCATTTCATCTTCTAAAGTCATACAATCAATAATAAGGTGTTCAAAATATTGAAAATCAATAATAACAGTTTTTGCATTTGTTACTCTTTTTTTGTTTTGTTTAATTAAGAAGCATTCAATTGTGTCTTGATTTTGCATACCATTAGCAGAAAACTTGTTTTTATCTGTGAGTTATCTTTTATAGTTGTGTTCTTTCTTGTGACTCAAGCATGTCTATGATGTAATTGAATACAGATGCAATACAGAAGACATTCTTCTCGAGATGGACCGAATATTGCGACCAGAAGGCGCTGTCATAATCCGCGATGAAGTCGATGTGTTAATTAAGGTAAAGAAGTTAATCGGAGGAATGAGATGGAATATGAAATTGGTCGATCATGAGGATGGTCCTCTTGTTCCTGAGAAAGTACTAATTGCTGTCAAACAGTATTGGGTTACTGATGGAAATTCCACATCAACACAATAA
Protein sequence

>MS.gene25479.t1

MAKPSAADNRTRSSVQIFIVVGLCCFFYILGAWQRSGFGKGDSIALEITKNNAECDVVPNLSFDSHHAGEVSQIDESNSNTKVFKPCDARYTDYTPCQDQRRAMTFPRENMNYRERHCPPEEEKLHCMIPAPKGYVTPFPWPKSRDYVPYANAPYKSLTVEKAIQNWIQYEGNVFRFPGGGTQFPQGADKYIDQLASVIPINDGTVRTALDTGCGVASWGAYLWSRNVVAMSFAPRDSHEAQVQFALERGVPAVIGVFGTIKLPYPSRAFDMAHCSRCLIPWGANDGMYMMEVDRVLRPGGYWVLSGPPINWKVNYKPWQRPKEELEEEQRKIEEVAKKLCWEKKSEKAEIAIWQKMTDTESCRSRQDDSSVEFCESSDPDDVWYKKMKACVTPTPKVSGGDLKPFPNRLYAIPPRVSSGSIPGVSSETYQDDNKMWKKHVNAYKKINSLLDSGRYRNIMDMNAGLGSFAAAIHSSKSWVMNVVPTIAEKSTLGVIYERGLIGIYHDWCEGFSTYPRTYDLIHANGLFSLYQDKCNTEDILLEMDRILRPEGAVIIRDEVDVLIKVKKLIGGMRWNMKLVDHEDGPLVPEKVLIAVKQYWVTDGNSTSTQ