Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene25741.t1 | GAU27734.1 | 86.4 | 103 | 11 | 2 | 8 | 110 | 19 | 118 | 1.40E-36 | 162.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene25741.t1 | A0A2Z6M5S5 | 86.4 | 103 | 11 | 2 | 8 | 110 | 19 | 118 | 1.0e-36 | 162.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050416 | MS.gene25741 | 0.848399 | 6.28E-60 | -1.69E-46 |
| MS.gene051959 | MS.gene25741 | 0.807641 | 4.58E-50 | -1.69E-46 |
| MS.gene051960 | MS.gene25741 | 0.809879 | 1.52E-50 | -1.69E-46 |
| MS.gene052091 | MS.gene25741 | 0.823131 | 1.63E-53 | -1.69E-46 |
| MS.gene057001 | MS.gene25741 | 0.803788 | 2.95E-49 | -1.69E-46 |
| MS.gene060237 | MS.gene25741 | 0.803946 | 2.73E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 46 sgRNAs with CRISPR-Local
Find 56 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGATTCATCATGGTTGATTC+AGG | 0.196823 | 3.2:-71352003 | MS.gene25741:CDS |
| CTGGCTGAGGTTGGTGAGCT+TGG | 0.290535 | 3.2:-71352024 | MS.gene25741:CDS |
| ACCCTAGCAGGCCTAGGGCT+TGG | 0.349369 | 3.2:+71352237 | None:intergenic |
| TTCAGATGGACCCAAGTTGC+TGG | 0.349830 | 3.2:-71352043 | MS.gene25741:CDS |
| CCTGCTAGGGTTGAGAAATT+AGG | 0.351690 | 3.2:-71352225 | MS.gene25741:CDS |
| AGGCCTAGGGCTTGGCCCAC+GGG | 0.363073 | 3.2:+71352245 | None:intergenic |
| CAGGCCTAGGGCTTGGCCCA+CGG | 0.392030 | 3.2:+71352244 | None:intergenic |
| GCTGCTGCAAAAGGTGCAAT+AGG | 0.418378 | 3.2:-71352183 | MS.gene25741:CDS |
| GATTCATCATGGTTGATTCA+GGG | 0.429834 | 3.2:-71352002 | MS.gene25741:CDS |
| AGCCCTTGATCTTCTTCGTA+TGG | 0.433702 | 3.2:-71352082 | MS.gene25741:CDS |
| TTCTCAACCCTAGCAGGCCT+AGG | 0.444711 | 3.2:+71352231 | None:intergenic |
| GGCCAAGCCCTAGGCCTGCT+AGG | 0.451902 | 3.2:-71352239 | MS.gene25741:CDS |
| AAGCCCGTGGGCCAAGCCCT+AGG | 0.457915 | 3.2:-71352248 | MS.gene25741:CDS |
| TTAGTAGTAATGGAAGTGTA+AGG | 0.460397 | 3.2:-71351955 | None:intergenic |
| GCCCTTGATCTTCTTCGTAT+GGG | 0.464225 | 3.2:-71352081 | MS.gene25741:CDS |
| CCTAATTTCTCAACCCTAGC+AGG | 0.472155 | 3.2:+71352225 | None:intergenic |
| GATGTGGCTGCTGCTGCAAA+AGG | 0.475844 | 3.2:-71352192 | MS.gene25741:CDS |
| ACCAACCTCAGCCAGCAACT+TGG | 0.483517 | 3.2:+71352032 | None:intergenic |
| TTCGTATGGGCTTTGACACA+CGG | 0.492946 | 3.2:-71352068 | MS.gene25741:CDS |
| GCCAAGCCCTAGGCCTGCTA+GGG | 0.502498 | 3.2:-71352238 | MS.gene25741:CDS |
| AGGGGTGGTGTTAGTAGTAA+TGG | 0.506379 | 3.2:-71351965 | MS.gene25741:CDS |
| CCCAAGTTGCTGGCTGAGGT+TGG | 0.527272 | 3.2:-71352033 | MS.gene25741:CDS |
| CCAACCTCAGCCAGCAACTT+GGG | 0.528451 | 3.2:+71352033 | None:intergenic |
| GAAGAAGATCAAGGGCTTCG+CGG | 0.529169 | 3.2:+71352088 | None:intergenic |
| AGCCCATACGAAGAAGATCA+AGG | 0.531159 | 3.2:+71352079 | None:intergenic |
| CACTACTAATTTCCGTGCCA+TGG | 0.531867 | 3.2:+71352140 | None:intergenic |
| AGCAACTTGGGTCCATCTGA+AGG | 0.541645 | 3.2:+71352045 | None:intergenic |
| TCTCAACCCTAGCAGGCCTA+GGG | 0.552947 | 3.2:+71352232 | None:intergenic |
| AAAAGGTGCAATAGGACCTC+CGG | 0.561409 | 3.2:-71352175 | MS.gene25741:CDS |
| TGGTGAGCTTGGATTCATCA+TGG | 0.561975 | 3.2:-71352013 | MS.gene25741:CDS |
| TGGACCCAAGTTGCTGGCTG+AGG | 0.570124 | 3.2:-71352037 | MS.gene25741:CDS |
| GCCGACTACTTTACTTAACA+CGG | 0.571600 | 3.2:+71352116 | None:intergenic |
| TTTGACACACGGCCTTCAGA+TGG | 0.574107 | 3.2:-71352057 | MS.gene25741:CDS |
| GGGTAAAGTTGAAGCAGGGG+TGG | 0.597668 | 3.2:-71351980 | MS.gene25741:CDS |
| GCCCATACGAAGAAGATCAA+GGG | 0.605404 | 3.2:+71352080 | None:intergenic |
| GCCATGGTACAACAATTCAC+CGG | 0.607187 | 3.2:+71352156 | None:intergenic |
| TTCATCATGGTTGATTCAGG+GGG | 0.612844 | 3.2:-71352000 | MS.gene25741:CDS |
| ATTCATCATGGTTGATTCAG+GGG | 0.614825 | 3.2:-71352001 | MS.gene25741:CDS |
| TGAATTGTTGTACCATGGCA+CGG | 0.621886 | 3.2:-71352152 | MS.gene25741:CDS |
| TCCGGTGAATTGTTGTACCA+TGG | 0.630340 | 3.2:-71352157 | MS.gene25741:CDS |
| AACGTTAATACACAAGTAGG+AGG | 0.633926 | 3.2:+71352381 | None:intergenic |
| TCATAGGATGGGAAGCCCGT+GGG | 0.636209 | 3.2:-71352260 | MS.gene25741:intron |
| TCCGTGTTAAGTAAAGTAGT+CGG | 0.660881 | 3.2:-71352117 | MS.gene25741:CDS |
| ATTAGGTGGTGATGATGATG+TGG | 0.666182 | 3.2:-71352208 | MS.gene25741:CDS |
| GCTAGGGTTGAGAAATTAGG+TGG | 0.673876 | 3.2:-71352222 | MS.gene25741:CDS |
| ATGGTACAACAATTCACCGG+AGG | 0.714463 | 3.2:+71352159 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| CAACACTACAATATGTATAG+TGG | + | chr3.2:71351996-71352015 | None:intergenic | 30.0% | |
| ! | CATAGATTGATTCATAGGAT+GGG | - | chr3.2:71352079-71352098 | MS.gene25741:CDS | 30.0% |
| ! | GACATCATAGATTGATTCAT+AGG | - | chr3.2:71352074-71352093 | MS.gene25741:CDS | 30.0% |
| ! | TCATAGATTGATTCATAGGA+TGG | - | chr3.2:71352078-71352097 | MS.gene25741:CDS | 30.0% |
| AACGTTAATACACAAGTAGG+AGG | + | chr3.2:71351972-71351991 | None:intergenic | 35.0% | |
| TCCGTGTTAAGTAAAGTAGT+CGG | - | chr3.2:71352233-71352252 | MS.gene25741:CDS | 35.0% | |
| ! | ATTCATCATGGTTGATTCAG+GGG | - | chr3.2:71352349-71352368 | MS.gene25741:intron | 35.0% |
| ! | GATTCATCATGGTTGATTCA+GGG | - | chr3.2:71352348-71352367 | MS.gene25741:intron | 35.0% |
| GCCGACTACTTTACTTAACA+CGG | + | chr3.2:71352237-71352256 | None:intergenic | 40.0% | |
| GGCAACGTTAATACACAAGT+AGG | + | chr3.2:71351975-71351994 | None:intergenic | 40.0% | |
| TGAATTGTTGTACCATGGCA+CGG | - | chr3.2:71352198-71352217 | MS.gene25741:CDS | 40.0% | |
| ! | ATTAGGTGGTGATGATGATG+TGG | - | chr3.2:71352142-71352161 | MS.gene25741:CDS | 40.0% |
| ! | GGATTCATCATGGTTGATTC+AGG | - | chr3.2:71352347-71352366 | MS.gene25741:intron | 40.0% |
| ! | GTTGTTGTTGAAGCTGTTGT+TGG | - | chr3.2:71352039-71352058 | MS.gene25741:CDS | 40.0% |
| ! | TGAAGCTGTTGTTGGTAGAA+AGG | - | chr3.2:71352047-71352066 | MS.gene25741:CDS | 40.0% |
| ! | TTCATCATGGTTGATTCAGG+GGG | - | chr3.2:71352350-71352369 | MS.gene25741:intron | 40.0% |
| AGCCCATACGAAGAAGATCA+AGG | + | chr3.2:71352274-71352293 | None:intergenic | 45.0% | |
| AGCCCTTGATCTTCTTCGTA+TGG | - | chr3.2:71352268-71352287 | MS.gene25741:intron | 45.0% | |
| ATGGTACAACAATTCACCGG+AGG | + | chr3.2:71352194-71352213 | None:intergenic | 45.0% | |
| CACTACTAATTTCCGTGCCA+TGG | + | chr3.2:71352213-71352232 | None:intergenic | 45.0% | |
| CCTAATTTCTCAACCCTAGC+AGG | + | chr3.2:71352128-71352147 | None:intergenic | 45.0% | |
| CCTGCTAGGGTTGAGAAATT+AGG | - | chr3.2:71352125-71352144 | MS.gene25741:CDS | 45.0% | |
| GCCATGGTACAACAATTCAC+CGG | + | chr3.2:71352197-71352216 | None:intergenic | 45.0% | |
| GCCCATACGAAGAAGATCAA+GGG | + | chr3.2:71352273-71352292 | None:intergenic | 45.0% | |
| GCCCTTGATCTTCTTCGTAT+GGG | - | chr3.2:71352269-71352288 | MS.gene25741:intron | 45.0% | |
| GCTAGGGTTGAGAAATTAGG+TGG | - | chr3.2:71352128-71352147 | MS.gene25741:CDS | 45.0% | |
| TCCGGTGAATTGTTGTACCA+TGG | - | chr3.2:71352193-71352212 | MS.gene25741:CDS | 45.0% | |
| TGGTGAGCTTGGATTCATCA+TGG | - | chr3.2:71352337-71352356 | MS.gene25741:intron | 45.0% | |
| ! | AAAAGGTGCAATAGGACCTC+CGG | - | chr3.2:71352175-71352194 | MS.gene25741:CDS | 45.0% |
| ! | AGGGGTGGTGTTAGTAGTAA+TGG | - | chr3.2:71352385-71352404 | MS.gene25741:intron | 45.0% |
| ! | TTCGTATGGGCTTTGACACA+CGG | - | chr3.2:71352282-71352301 | MS.gene25741:intron | 45.0% |
| AGCAACTTGGGTCCATCTGA+AGG | + | chr3.2:71352308-71352327 | None:intergenic | 50.0% | |
| AGGGGGTAAAGTTGAAGCAG+GGG | - | chr3.2:71352367-71352386 | MS.gene25741:intron | 50.0% | |
| CAGGGGGTAAAGTTGAAGCA+GGG | - | chr3.2:71352366-71352385 | MS.gene25741:intron | 50.0% | |
| GAAGAAGATCAAGGGCTTCG+CGG | + | chr3.2:71352265-71352284 | None:intergenic | 50.0% | |
| TCAGGGGGTAAAGTTGAAGC+AGG | - | chr3.2:71352365-71352384 | MS.gene25741:intron | 50.0% | |
| TTCAGATGGACCCAAGTTGC+TGG | - | chr3.2:71352307-71352326 | MS.gene25741:intron | 50.0% | |
| TTTGACACACGGCCTTCAGA+TGG | - | chr3.2:71352293-71352312 | MS.gene25741:intron | 50.0% | |
| ! | GCTGCTGCAAAAGGTGCAAT+AGG | - | chr3.2:71352167-71352186 | MS.gene25741:CDS | 50.0% |
| ACCAACCTCAGCCAGCAACT+TGG | + | chr3.2:71352321-71352340 | None:intergenic | 55.0% | |
| CCAACCTCAGCCAGCAACTT+GGG | + | chr3.2:71352320-71352339 | None:intergenic | 55.0% | |
| GATGTGGCTGCTGCTGCAAA+AGG | - | chr3.2:71352158-71352177 | MS.gene25741:CDS | 55.0% | |
| GGGTAAAGTTGAAGCAGGGG+TGG | - | chr3.2:71352370-71352389 | MS.gene25741:intron | 55.0% | |
| TCATAGGATGGGAAGCCCGT+GGG | - | chr3.2:71352090-71352109 | MS.gene25741:CDS | 55.0% | |
| TCTCAACCCTAGCAGGCCTA+GGG | + | chr3.2:71352121-71352140 | None:intergenic | 55.0% | |
| TTCATAGGATGGGAAGCCCG+TGG | - | chr3.2:71352089-71352108 | MS.gene25741:CDS | 55.0% | |
| TTCTCAACCCTAGCAGGCCT+AGG | + | chr3.2:71352122-71352141 | None:intergenic | 55.0% | |
| ! | CCCAAGTTGCTGGCTGAGGT+TGG | - | chr3.2:71352317-71352336 | MS.gene25741:intron | 60.0% |
| ! | TGGACCCAAGTTGCTGGCTG+AGG | - | chr3.2:71352313-71352332 | MS.gene25741:intron | 60.0% |
| !! | CTGGCTGAGGTTGGTGAGCT+TGG | - | chr3.2:71352326-71352345 | MS.gene25741:intron | 60.0% |
| ACCCTAGCAGGCCTAGGGCT+TGG | + | chr3.2:71352116-71352135 | None:intergenic | 65.0% | |
| GCCAAGCCCTAGGCCTGCTA+GGG | - | chr3.2:71352112-71352131 | MS.gene25741:CDS | 65.0% | |
| AAGCCCGTGGGCCAAGCCCT+AGG | - | chr3.2:71352102-71352121 | MS.gene25741:CDS | 70.0% | |
| AGGCCTAGGGCTTGGCCCAC+GGG | + | chr3.2:71352108-71352127 | None:intergenic | 70.0% | |
| CAGGCCTAGGGCTTGGCCCA+CGG | + | chr3.2:71352109-71352128 | None:intergenic | 70.0% | |
| GGCCAAGCCCTAGGCCTGCT+AGG | - | chr3.2:71352111-71352130 | MS.gene25741:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.2 | gene | 71351957 | 71352415 | 71351957 | ID=MS.gene25741 |
| chr3.2 | mRNA | 71351957 | 71352415 | 71351957 | ID=MS.gene25741.t1;Parent=MS.gene25741 |
| chr3.2 | exon | 71352394 | 71352415 | 71352394 | ID=MS.gene25741.t1.exon1;Parent=MS.gene25741.t1 |
| chr3.2 | CDS | 71352394 | 71352415 | 71352394 | ID=cds.MS.gene25741.t1;Parent=MS.gene25741.t1 |
| chr3.2 | exon | 71351957 | 71352267 | 71351957 | ID=MS.gene25741.t1.exon2;Parent=MS.gene25741.t1 |
| chr3.2 | CDS | 71351957 | 71352267 | 71351957 | ID=cds.MS.gene25741.t1;Parent=MS.gene25741.t1 |
| Gene Sequence |
| Protein sequence |
