Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene26110.t1 | XP_003616291.1 | 88.5 | 882 | 100 | 1 | 1 | 882 | 106 | 986 | 0.00E+00 | 1383.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene26110.t1 | Q0WNW4 | 43.5 | 441 | 196 | 10 | 424 | 860 | 272 | 663 | 2.1e-46 | 189.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene26110.t1 | G7K1Q1 | 88.5 | 882 | 100 | 1 | 1 | 882 | 106 | 986 | 0.0e+00 | 1383.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049418 | MS.gene26110 | 0.800123 | 1.67E-48 | -1.69E-46 |
| MS.gene049711 | MS.gene26110 | 0.804503 | 2.09E-49 | -1.69E-46 |
| MS.gene051720 | MS.gene26110 | 0.808897 | 2.47E-50 | -1.69E-46 |
| MS.gene052516 | MS.gene26110 | 0.802696 | 4.96E-49 | -1.69E-46 |
| MS.gene052732 | MS.gene26110 | 0.816713 | 4.80E-52 | -1.69E-46 |
| MS.gene053144 | MS.gene26110 | 0.842974 | 1.86E-58 | -1.69E-46 |
| MS.gene053536 | MS.gene26110 | 0.805259 | 1.46E-49 | -1.69E-46 |
| MS.gene053538 | MS.gene26110 | 0.812365 | 4.40E-51 | -1.69E-46 |
| MS.gene054584 | MS.gene26110 | 0.854765 | 9.90E-62 | -1.69E-46 |
| MS.gene055465 | MS.gene26110 | 0.848381 | 6.35E-60 | -1.69E-46 |
| MS.gene055466 | MS.gene26110 | 0.839873 | 1.22E-57 | -1.69E-46 |
| MS.gene055468 | MS.gene26110 | 0.839044 | 2.00E-57 | -1.69E-46 |
| MS.gene055469 | MS.gene26110 | 0.826963 | 2.03E-54 | -1.69E-46 |
| MS.gene055537 | MS.gene26110 | 0.850346 | 1.80E-60 | -1.69E-46 |
| MS.gene055580 | MS.gene26110 | -0.834714 | 2.56E-56 | -1.69E-46 |
| MS.gene056043 | MS.gene26110 | -0.822225 | 2.65E-53 | -1.69E-46 |
| MS.gene056308 | MS.gene26110 | 0.805958 | 1.04E-49 | -1.69E-46 |
| MS.gene058087 | MS.gene26110 | 0.814136 | 1.80E-51 | -1.69E-46 |
| MS.gene058668 | MS.gene26110 | 0.826632 | 2.44E-54 | -1.69E-46 |
| MS.gene059424 | MS.gene26110 | 0.800679 | 1.29E-48 | -1.69E-46 |
| MS.gene059426 | MS.gene26110 | 0.835385 | 1.73E-56 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene26110.t1 | MTR_5g078300 | 96.145 | 882 | 33 | 1 | 1 | 882 | 106 | 986 | 0.0 | 1713 |
| MS.gene26110.t1 | MTR_1g070350 | 35.606 | 396 | 219 | 12 | 475 | 861 | 496 | 864 | 1.47e-39 | 158 |
| MS.gene26110.t1 | MTR_7g115350 | 34.336 | 399 | 215 | 14 | 474 | 861 | 504 | 866 | 1.42e-34 | 142 |
| MS.gene26110.t1 | MTR_0168s0060 | 61.616 | 99 | 38 | 0 | 513 | 611 | 331 | 429 | 6.68e-23 | 103 |
| MS.gene26110.t1 | MTR_4g011540 | 49.485 | 97 | 49 | 0 | 513 | 609 | 286 | 382 | 3.80e-16 | 82.4 |
| MS.gene26110.t1 | MTR_4g107310 | 48.611 | 144 | 68 | 3 | 468 | 611 | 290 | 427 | 4.55e-16 | 82.8 |
| MS.gene26110.t1 | MTR_4g011550 | 48.352 | 91 | 47 | 0 | 520 | 610 | 281 | 371 | 6.01e-16 | 82.4 |
| MS.gene26110.t1 | MTR_4g093150 | 37.037 | 108 | 68 | 0 | 508 | 615 | 68 | 175 | 1.88e-14 | 73.6 |
| MS.gene26110.t1 | MTR_7g107000 | 40.385 | 104 | 62 | 0 | 516 | 619 | 70 | 173 | 3.00e-14 | 76.6 |
| MS.gene26110.t1 | MTR_1g054835 | 42.308 | 104 | 60 | 0 | 516 | 619 | 65 | 168 | 7.55e-14 | 75.5 |
| MS.gene26110.t1 | MTR_4g130400 | 40.952 | 105 | 62 | 0 | 513 | 617 | 6 | 110 | 1.16e-12 | 71.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene26110.t1 | AT5G16720 | 46.212 | 396 | 170 | 7 | 466 | 860 | 310 | 663 | 1.33e-87 | 293 |
| MS.gene26110.t1 | AT1G70750 | 47.453 | 373 | 155 | 11 | 495 | 860 | 400 | 738 | 7.31e-73 | 254 |
| MS.gene26110.t1 | AT1G08800 | 54.015 | 137 | 57 | 3 | 475 | 611 | 858 | 988 | 4.35e-30 | 129 |
| MS.gene26110.t1 | AT1G08800 | 54.015 | 137 | 57 | 3 | 475 | 611 | 858 | 988 | 4.35e-30 | 129 |
| MS.gene26110.t1 | AT1G08800 | 54.015 | 137 | 57 | 3 | 475 | 611 | 858 | 988 | 4.35e-30 | 129 |
| MS.gene26110.t1 | AT1G08800 | 54.015 | 137 | 57 | 3 | 475 | 611 | 858 | 988 | 4.35e-30 | 129 |
| MS.gene26110.t1 | AT2G30690 | 60.784 | 102 | 40 | 0 | 513 | 614 | 613 | 714 | 4.23e-27 | 118 |
| MS.gene26110.t1 | AT2G30690 | 60.784 | 102 | 40 | 0 | 513 | 614 | 613 | 714 | 4.23e-27 | 118 |
| MS.gene26110.t1 | AT1G74830 | 40.000 | 210 | 118 | 2 | 513 | 722 | 302 | 503 | 6.51e-26 | 113 |
| MS.gene26110.t1 | AT1G18990 | 60.185 | 108 | 41 | 1 | 502 | 609 | 292 | 397 | 1.35e-21 | 100 |
| MS.gene26110.t1 | AT4G13630 | 41.135 | 141 | 69 | 3 | 480 | 616 | 195 | 325 | 1.19e-17 | 87.8 |
| MS.gene26110.t1 | AT4G13630 | 41.135 | 141 | 69 | 3 | 480 | 616 | 195 | 325 | 1.19e-17 | 87.8 |
| MS.gene26110.t1 | AT5G06560 | 40.000 | 100 | 60 | 0 | 516 | 615 | 74 | 173 | 5.76e-16 | 82.4 |
| MS.gene26110.t1 | AT3G54740 | 39.604 | 101 | 61 | 0 | 519 | 619 | 77 | 177 | 1.70e-15 | 80.5 |
| MS.gene26110.t1 | AT3G54740 | 39.604 | 101 | 61 | 0 | 519 | 619 | 77 | 177 | 1.70e-15 | 80.5 |
| MS.gene26110.t1 | AT3G54740 | 39.604 | 101 | 61 | 0 | 519 | 619 | 77 | 177 | 1.70e-15 | 80.5 |
| MS.gene26110.t1 | AT3G54740 | 39.604 | 101 | 61 | 0 | 519 | 619 | 77 | 177 | 1.70e-15 | 80.5 |
| MS.gene26110.t1 | AT3G54740 | 39.604 | 101 | 61 | 0 | 519 | 619 | 29 | 129 | 2.46e-15 | 79.3 |
| MS.gene26110.t1 | AT3G54740 | 39.604 | 101 | 61 | 0 | 519 | 619 | 29 | 129 | 2.46e-15 | 79.3 |
| MS.gene26110.t1 | AT3G54740 | 39.604 | 101 | 61 | 0 | 519 | 619 | 29 | 129 | 2.46e-15 | 79.3 |
| MS.gene26110.t1 | AT3G54740 | 39.604 | 101 | 61 | 0 | 519 | 619 | 29 | 129 | 2.46e-15 | 79.3 |
| MS.gene26110.t1 | AT1G04890 | 42.969 | 128 | 70 | 2 | 485 | 610 | 82 | 208 | 2.21e-14 | 76.6 |
| MS.gene26110.t1 | AT4G13160 | 47.872 | 94 | 49 | 0 | 516 | 609 | 117 | 210 | 1.81e-13 | 72.4 |
Find 120 sgRNAs with CRISPR-Local
Find 152 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTTATCTGTTGAAGCCTTCT+TGG | 0.160198 | 5.4:-56773503 | MS.gene26110:CDS |
| GTGAAAATTGCTTGGCTTCT+AGG | 0.206836 | 5.4:-56773677 | MS.gene26110:CDS |
| CTTGTTCTTCCATCATTCTT+TGG | 0.217588 | 5.4:+56771313 | None:intergenic |
| AAGCAACCAATTGTTCTTCT+TGG | 0.220911 | 5.4:+56770781 | None:intergenic |
| ACAGATAAGGAGGGTAAATT+TGG | 0.246430 | 5.4:+56773517 | None:intergenic |
| TGTTTACCTTGTGCATTATC+TGG | 0.252484 | 5.4:+56772775 | None:intergenic |
| TCTGCTGACCTAGGAGTTTC+AGG | 0.253809 | 5.4:+56771624 | None:intergenic |
| ATCCGACAATGACAGAAATT+TGG | 0.280174 | 5.4:-56772489 | MS.gene26110:CDS |
| CAGAAATTTGGAAGCAGATA+TGG | 0.283479 | 5.4:-56772477 | MS.gene26110:CDS |
| TTCTCTGCTTCATCTAAATA+TGG | 0.289720 | 5.4:+56770742 | None:intergenic |
| CAATGTGAAACCTGCATAAT+AGG | 0.290320 | 5.4:+56773180 | None:intergenic |
| CATGAGAAACATGATGAAAA+TGG | 0.306053 | 5.4:-56773585 | MS.gene26110:CDS |
| AATGGAGCTTGAAGAAAGTT+TGG | 0.325600 | 5.4:-56772896 | MS.gene26110:CDS |
| AGAATTTCTCCAACATTGTA+TGG | 0.334868 | 5.4:-56770548 | MS.gene26110:CDS |
| ATAGAATCTATTAACTTAAC+AGG | 0.364312 | 5.4:+56773081 | None:intergenic |
| AGAGGAAGTATTGATCAAAA+TGG | 0.367658 | 5.4:-56772944 | MS.gene26110:CDS |
| AGAAGGAATTGGAAGAGTAT+AGG | 0.372537 | 5.4:-56771215 | MS.gene26110:CDS |
| TGATGATACCGTTTCTAACT+TGG | 0.372874 | 5.4:-56771022 | MS.gene26110:CDS |
| CTATCCATTTCACTTATCAT+AGG | 0.373876 | 5.4:+56770640 | None:intergenic |
| GAAGGAATTGGAAGAGTATA+GGG | 0.377022 | 5.4:-56771214 | MS.gene26110:CDS |
| AGAGGAAAAGATAATTTCAT+TGG | 0.377885 | 5.4:-56770896 | MS.gene26110:CDS |
| TGAATTACGCCTTAAGAACT+TGG | 0.379118 | 5.4:-56770446 | MS.gene26110:CDS |
| GCAAGAACTTGAGAAGGAAT+TGG | 0.385367 | 5.4:-56771226 | MS.gene26110:CDS |
| CACAAAGAAGACTATTCTAA+TGG | 0.388166 | 5.4:-56770826 | MS.gene26110:CDS |
| AAAATGGAGAGAGTTTAAAT+AGG | 0.391672 | 5.4:-56773569 | MS.gene26110:CDS |
| GTAGTTCAAATTTCTGTGAA+TGG | 0.392563 | 5.4:-56772829 | MS.gene26110:CDS |
| CATGAAACTGAAAACTCATT+TGG | 0.392989 | 5.4:-56773645 | MS.gene26110:CDS |
| AGATCAACTCAAAGCATTAG+AGG | 0.392996 | 5.4:-56770914 | MS.gene26110:CDS |
| GATCATTGTCCAAGTTCTTA+AGG | 0.394095 | 5.4:+56770437 | None:intergenic |
| TTGTAATCATTGCCATTGTT+TGG | 0.398108 | 5.4:+56771382 | None:intergenic |
| TTCCAAATTTCTGTCATTGT+CGG | 0.401114 | 5.4:+56772487 | None:intergenic |
| AAACTTGAACTCTGATGAAA+AGG | 0.403932 | 5.4:-56773274 | MS.gene26110:CDS |
| TGTATGGGATCCATACAAAA+TGG | 0.406925 | 5.4:-56770532 | MS.gene26110:CDS |
| ACGCTTGATTGCGGTTCAAC+TGG | 0.410737 | 5.4:+56772973 | None:intergenic |
| TTCTGATGCTCCTATTATGC+AGG | 0.413743 | 5.4:-56773190 | MS.gene26110:CDS |
| CTCTTTGAGAAAACAGAATC+AGG | 0.416556 | 5.4:-56771570 | MS.gene26110:CDS |
| AGTTCCTATGATAAGTGAAA+TGG | 0.425926 | 5.4:-56770644 | MS.gene26110:CDS |
| TCTATATCTTTAGAAGTTGA+AGG | 0.427647 | 5.4:-56772868 | MS.gene26110:CDS |
| AAGAAGAACAATTGGTTGCT+TGG | 0.429766 | 5.4:-56770779 | MS.gene26110:CDS |
| ATTTGCATTTGATAGAGAAT+TGG | 0.437319 | 5.4:-56770701 | MS.gene26110:CDS |
| TGTAGAGAGAACAAACATAA+TGG | 0.439193 | 5.4:-56772914 | MS.gene26110:CDS |
| GTTAGAAACGGTATCATCAT+TGG | 0.443026 | 5.4:+56771026 | None:intergenic |
| TGCACACAAGAAGATGAATC+TGG | 0.444733 | 5.4:-56772596 | MS.gene26110:CDS |
| TAGTACTTTAGAAGAATTTG+AGG | 0.444834 | 5.4:-56770956 | MS.gene26110:CDS |
| CAGTGTAAATGATAATGAAA+TGG | 0.445770 | 5.4:-56771742 | MS.gene26110:CDS |
| AGCCGGTCGATTGTTAAAAC+CGG | 0.449648 | 5.4:+56771495 | None:intergenic |
| TTGAAGCCTTCTTGGTATGA+TGG | 0.459164 | 5.4:-56773495 | MS.gene26110:CDS |
| TTCACCAAGCAAAGACTTGT+AGG | 0.462618 | 5.4:-56770392 | None:intergenic |
| TGACATGCATTGACTTGAGT+TGG | 0.463755 | 5.4:+56771998 | None:intergenic |
| CAATCTCCAAGAAGAACAAT+TGG | 0.464940 | 5.4:-56770787 | MS.gene26110:CDS |
| AAGATAATTTCATTGGAAGA+TGG | 0.470072 | 5.4:-56770889 | MS.gene26110:CDS |
| GATGAGAGTTCATCAAGATA+TGG | 0.474936 | 5.4:-56770853 | MS.gene26110:CDS |
| GAGTTGGTTGGTTCTTCAGA+TGG | 0.476219 | 5.4:+56772014 | None:intergenic |
| AGCACTGAGAGCTCTTTGTT+CGG | 0.478445 | 5.4:+56771455 | None:intergenic |
| TATGCCTACAAGTCTTTGCT+TGG | 0.481502 | 5.4:+56770388 | None:intergenic |
| AACTGAACTCGATCGATCCT+CGG | 0.488164 | 5.4:+56773299 | None:intergenic |
| CAAGAAGGCTTCAACAGATA+AGG | 0.490047 | 5.4:+56773504 | None:intergenic |
| ACTCATTTGGTATGAGACAT+AGG | 0.495233 | 5.4:-56773632 | MS.gene26110:CDS |
| TACAAGGCTGCAGGAAGAAA+AGG | 0.495890 | 5.4:-56771364 | MS.gene26110:CDS |
| TGAATGCAACTTAACTATAG+TGG | 0.498253 | 5.4:+56770411 | None:intergenic |
| TGAATCATGCAAATCGAATG+AGG | 0.507100 | 5.4:-56773043 | MS.gene26110:CDS |
| ATGTTGAGAAGAATGAAAGA+TGG | 0.509499 | 5.4:-56771153 | MS.gene26110:CDS |
| ATAAACTTCCTGAAACTCCT+AGG | 0.514133 | 5.4:-56771632 | MS.gene26110:CDS |
| TCTTGTTCTTGTTGCAACAC+CGG | 0.514596 | 5.4:-56771108 | MS.gene26110:CDS |
| GGCAATGATTACAAGGCTGC+AGG | 0.518083 | 5.4:-56771373 | MS.gene26110:CDS |
| AGGTTGATCGCGTTTATGAC+AGG | 0.518142 | 5.4:-56770594 | MS.gene26110:CDS |
| TGATACCGTTTCTAACTTGG+AGG | 0.518834 | 5.4:-56771019 | MS.gene26110:CDS |
| ATACAAAATGGTGGTGATGA+AGG | 0.519176 | 5.4:-56770520 | MS.gene26110:CDS |
| TGCATTATCTGGTTCTTCTG+TGG | 0.527662 | 5.4:+56772786 | None:intergenic |
| TGACAGTTCATCGGTGTAGC+CGG | 0.531224 | 5.4:+56771089 | None:intergenic |
| AGAAAAGGCGGCAATGCAGA+TGG | 0.535335 | 5.4:-56771349 | MS.gene26110:CDS |
| TGAGGATCTAGAGGAGGGGA+AGG | 0.539564 | 5.4:-56773025 | MS.gene26110:CDS |
| CATTTCATTATCATTTACAC+TGG | 0.540128 | 5.4:+56771743 | None:intergenic |
| CCATCAATCATCAAAATGCT+CGG | 0.540613 | 5.4:-56771676 | MS.gene26110:CDS |
| CTATATCTTTAGAAGTTGAA+GGG | 0.544204 | 5.4:-56772867 | MS.gene26110:CDS |
| GTATGGATCCCATACAATGT+TGG | 0.545411 | 5.4:+56770539 | None:intergenic |
| ATGCATTGACTTGAGTTGGT+TGG | 0.546919 | 5.4:+56772002 | None:intergenic |
| AGAGAAGCAAGAACTTGAGA+AGG | 0.550787 | 5.4:-56771232 | MS.gene26110:CDS |
| GAAAGTGTGTATTGAAGAAG+AGG | 0.550976 | 5.4:-56770614 | MS.gene26110:CDS |
| TCAATAGAAGATGACAAAGA+AGG | 0.551727 | 5.4:-56773438 | MS.gene26110:CDS |
| AAATGGTGGTGATGAAGGAA+AGG | 0.555471 | 5.4:-56770515 | MS.gene26110:CDS |
| TTGGTTGGTTCTTCAGATGG+AGG | 0.560112 | 5.4:+56772017 | None:intergenic |
| GGATGGTAGCGTTGCAAGTG+AGG | 0.560662 | 5.4:-56771532 | MS.gene26110:CDS |
| ATTGAATTTGAGATAAACAA+TGG | 0.561453 | 5.4:-56773405 | MS.gene26110:CDS |
| ATTACAATACCAAAGAATGA+TGG | 0.564697 | 5.4:-56771322 | MS.gene26110:CDS |
| AGATCATGATCATGATGAAG+AGG | 0.566217 | 5.4:-56773379 | MS.gene26110:CDS |
| GAATTTCTCCAACATTGTAT+GGG | 0.568122 | 5.4:-56770547 | MS.gene26110:CDS |
| TTTGGTATGAGACATAGGAT+TGG | 0.570047 | 5.4:-56773627 | MS.gene26110:CDS |
| TGAGGTAGAATACAGCGATC+CGG | 0.570543 | 5.4:-56771514 | MS.gene26110:CDS |
| TATCTCCTCCAAGTTAGAAA+CGG | 0.572085 | 5.4:+56771014 | None:intergenic |
| TAGTTTCCATCATACCAAGA+AGG | 0.574251 | 5.4:+56773489 | None:intergenic |
| TGCAACTTAACTATAGTGGA+TGG | 0.575938 | 5.4:+56770415 | None:intergenic |
| ATAAAATCATCAACAGCACT+TGG | 0.577726 | 5.4:+56773222 | None:intergenic |
| TAGCATGTGTGAAAATTGCT+TGG | 0.580838 | 5.4:-56773685 | None:intergenic |
| GAAGGCTTCAACAGATAAGG+AGG | 0.588495 | 5.4:+56773507 | None:intergenic |
| GCAATCAAGCGTCTTAGAAG+AGG | 0.588801 | 5.4:-56772962 | MS.gene26110:CDS |
| AAACAATGGCAATGATTACA+AGG | 0.589570 | 5.4:-56771380 | MS.gene26110:CDS |
| TCGAATGAGGATCTAGAGGA+GGG | 0.592079 | 5.4:-56773030 | MS.gene26110:CDS |
| ACTTGCAACGCTACCATCCA+CGG | 0.593320 | 5.4:+56771536 | None:intergenic |
| TTGGTTAGTAGCTATTGCTG+AGG | 0.595521 | 5.4:+56771401 | None:intergenic |
| ATCTTCTTCAAGTGATAGTG+AGG | 0.602149 | 5.4:-56771967 | MS.gene26110:CDS |
| AAGATCAACTGACAGTTCAT+CGG | 0.602771 | 5.4:+56771080 | None:intergenic |
| AAGCTTGTGACGATGATGAT+CGG | 0.602945 | 5.4:-56773110 | MS.gene26110:CDS |
| TCTTCTAAGACGCTTGATTG+CGG | 0.606157 | 5.4:+56772964 | None:intergenic |
| ATCGAATGAGGATCTAGAGG+AGG | 0.610877 | 5.4:-56773031 | MS.gene26110:CDS |
| TATCTTCAGAGAGGTGGCCG+AGG | 0.614505 | 5.4:-56773316 | MS.gene26110:CDS |
| TGCATTGCATCTGCTGACCT+AGG | 0.622751 | 5.4:+56771615 | None:intergenic |
| TGTTTCTCATGACTTAACCA+TGG | 0.628154 | 5.4:+56773597 | None:intergenic |
| GGAGCAGAAGAATCCGTGGA+TGG | 0.629443 | 5.4:-56771549 | MS.gene26110:CDS |
| CGAATGAGGATCTAGAGGAG+GGG | 0.631511 | 5.4:-56773029 | MS.gene26110:CDS |
| ATGCAATGCACTACATGCAG+AGG | 0.632820 | 5.4:-56771602 | MS.gene26110:CDS |
| ATCAGGAGCAGAAGAATCCG+TGG | 0.633581 | 5.4:-56771553 | MS.gene26110:CDS |
| CAAATCGAATGAGGATCTAG+AGG | 0.636941 | 5.4:-56773034 | MS.gene26110:CDS |
| AATAGCTACTAACCAAACAA+TGG | 0.642191 | 5.4:-56771394 | MS.gene26110:CDS |
| TGGATCAAGTGATGATGATG+AGG | 0.658998 | 5.4:-56772576 | MS.gene26110:CDS |
| GAACAAGAAAATGCTCCACC+AGG | 0.665638 | 5.4:-56772452 | MS.gene26110:intron |
| AAGGCTTCAACAGATAAGGA+GGG | 0.667591 | 5.4:+56773508 | None:intergenic |
| AAGGCTGCAGGAAGAAAAGG+CGG | 0.674245 | 5.4:-56771361 | MS.gene26110:CDS |
| GAAGAACCAGATAATGCACA+AGG | 0.705101 | 5.4:-56772781 | MS.gene26110:intron |
| ATGGGATCCATACAAAATGG+TGG | 0.735054 | 5.4:-56770529 | MS.gene26110:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AGTTTAGATAGATTGAAAAA+AGG | + | chr5.4:56772277-56772296 | None:intergenic | 20.0% |
| !! | ATAGAATCTATTAACTTAAC+AGG | + | chr5.4:56770996-56771015 | None:intergenic | 20.0% |
| !! | TTATGAAATTAGCAAATTCT+TGG | - | chr5.4:56771404-56771423 | MS.gene26110:CDS | 20.0% |
| !!! | ATTGAATTTGAGATAAACAA+TGG | - | chr5.4:56770669-56770688 | MS.gene26110:CDS | 20.0% |
| ! | AAAATGGAGAGAGTTTAAAT+AGG | - | chr5.4:56770505-56770524 | MS.gene26110:CDS | 25.0% |
| ! | AAGATAATTTCATTGGAAGA+TGG | - | chr5.4:56773185-56773204 | MS.gene26110:CDS | 25.0% |
| ! | AATAAACTGAAAACAGTCTA+TGG | + | chr5.4:56771828-56771847 | None:intergenic | 25.0% |
| ! | AATATCAGCTGAAAAAATTG+AGG | - | chr5.4:56772365-56772384 | MS.gene26110:intron | 25.0% |
| ! | AGAGGAAAAGATAATTTCAT+TGG | - | chr5.4:56773178-56773197 | MS.gene26110:CDS | 25.0% |
| ! | ATTACAATACCAAAGAATGA+TGG | - | chr5.4:56772752-56772771 | MS.gene26110:intron | 25.0% |
| ! | ATTTGCATTTGATAGAGAAT+TGG | - | chr5.4:56773373-56773392 | MS.gene26110:CDS | 25.0% |
| ! | CATGAATTATTAATACCGTT+TGG | + | chr5.4:56772007-56772026 | None:intergenic | 25.0% |
| ! | CATTTCATTATCATTTACAC+TGG | + | chr5.4:56772334-56772353 | None:intergenic | 25.0% |
| ! | GTACTAGATATAAAAACTCA+CGG | + | chr5.4:56772185-56772204 | None:intergenic | 25.0% |
| !! | CAGTGTAAATGATAATGAAA+TGG | - | chr5.4:56772332-56772351 | MS.gene26110:intron | 25.0% |
| !! | CTATATCTTTAGAAGTTGAA+GGG | - | chr5.4:56771207-56771226 | MS.gene26110:CDS | 25.0% |
| !! | GAATGATGATGTTAGATATA+TGG | - | chr5.4:56771378-56771397 | MS.gene26110:CDS | 25.0% |
| !! | TAGTACTTTAGAAGAATTTG+AGG | - | chr5.4:56773118-56773137 | MS.gene26110:CDS | 25.0% |
| !! | TCTATATCTTTAGAAGTTGA+AGG | - | chr5.4:56771206-56771225 | MS.gene26110:CDS | 25.0% |
| !! | TTTTCAGTTTCATGATGATT+TGG | + | chr5.4:56770423-56770442 | None:intergenic | 25.0% |
| !!! | ATCATTTACACTGGTTTTAT+CGG | + | chr5.4:56772325-56772344 | None:intergenic | 25.0% |
| !!! | CTGAGAAATGAATTTTTCAA+AGG | + | chr5.4:56771539-56771558 | None:intergenic | 25.0% |
| AAACAATGGCAATGATTACA+AGG | - | chr5.4:56772694-56772713 | MS.gene26110:intron | 30.0% | |
| AAACTTGAACTCTGATGAAA+AGG | - | chr5.4:56770800-56770819 | MS.gene26110:CDS | 30.0% | |
| AATAGCTACTAACCAAACAA+TGG | - | chr5.4:56772680-56772699 | MS.gene26110:intron | 30.0% | |
| AGAATTTCTCCAACATTGTA+TGG | - | chr5.4:56773526-56773545 | MS.gene26110:CDS | 30.0% | |
| AGAGTATAGGGAAAAAGTTA+TGG | - | chr5.4:56772872-56772891 | MS.gene26110:CDS | 30.0% | |
| AGTTCCTATGATAAGTGAAA+TGG | - | chr5.4:56773430-56773449 | MS.gene26110:CDS | 30.0% | |
| ATAAAATCATCAACAGCACT+TGG | + | chr5.4:56770855-56770874 | None:intergenic | 30.0% | |
| ATGTTGAGAAGAATGAAAGA+TGG | - | chr5.4:56772921-56772940 | MS.gene26110:CDS | 30.0% | |
| CACAAAGAAGACTATTCTAA+TGG | - | chr5.4:56773248-56773267 | MS.gene26110:CDS | 30.0% | |
| CATGAAACTGAAAACTCATT+TGG | - | chr5.4:56770429-56770448 | MS.gene26110:CDS | 30.0% | |
| CATGAGAAACATGATGAAAA+TGG | - | chr5.4:56770489-56770508 | MS.gene26110:CDS | 30.0% | |
| CATTCAGATGTTTATCCAAA+CGG | - | chr5.4:56771989-56772008 | MS.gene26110:CDS | 30.0% | |
| CTATCCATTTCACTTATCAT+AGG | + | chr5.4:56773437-56773456 | None:intergenic | 30.0% | |
| CTTCATTTATCACACATGTT+TGG | - | chr5.4:56771730-56771749 | MS.gene26110:CDS | 30.0% | |
| GAATTTCTCCAACATTGTAT+GGG | - | chr5.4:56773527-56773546 | MS.gene26110:CDS | 30.0% | |
| GTAGTTCAAATTTCTGTGAA+TGG | - | chr5.4:56771245-56771264 | MS.gene26110:CDS | 30.0% | |
| TCAATAGAAGATGACAAAGA+AGG | - | chr5.4:56770636-56770655 | MS.gene26110:CDS | 30.0% | |
| TGAATGCAACTTAACTATAG+TGG | + | chr5.4:56773666-56773685 | None:intergenic | 30.0% | |
| TGTAGAGAGAACAAACATAA+TGG | - | chr5.4:56771160-56771179 | MS.gene26110:CDS | 30.0% | |
| TTCCAAATTTCTGTCATTGT+CGG | + | chr5.4:56771590-56771609 | None:intergenic | 30.0% | |
| TTCTCTGCTTCATCTAAATA+TGG | + | chr5.4:56773335-56773354 | None:intergenic | 30.0% | |
| TTGTAATCATTGCCATTGTT+TGG | + | chr5.4:56772695-56772714 | None:intergenic | 30.0% | |
| TTTATCACACATGTTTGGAT+CGG | - | chr5.4:56771735-56771754 | MS.gene26110:CDS | 30.0% | |
| ! | GAGAAGATTCAAACTTTTGT+CGG | - | chr5.4:56771071-56771090 | MS.gene26110:CDS | 30.0% |
| ! | TGATGATGTTAGATATATGG+TGG | - | chr5.4:56771381-56771400 | MS.gene26110:CDS | 30.0% |
| ! | TGCTTGATGTATAGAGTTTT+TGG | - | chr5.4:56771327-56771346 | MS.gene26110:CDS | 30.0% |
| ! | TTCAGCTGATATTGTTTTCT+CGG | + | chr5.4:56772358-56772377 | None:intergenic | 30.0% |
| !! | AGAGGAAGTATTGATCAAAA+TGG | - | chr5.4:56771130-56771149 | MS.gene26110:CDS | 30.0% |
| !! | TGAAAGTTTTATCTTCAGAG+AGG | - | chr5.4:56770749-56770768 | MS.gene26110:CDS | 30.0% |
| !!! | ATAGGATTGGTTTTATTCCA+TGG | - | chr5.4:56770460-56770479 | MS.gene26110:CDS | 30.0% |
| AAGAAGAACAATTGGTTGCT+TGG | - | chr5.4:56773295-56773314 | MS.gene26110:CDS | 35.0% | |
| AAGATCAACTGACAGTTCAT+CGG | + | chr5.4:56772997-56773016 | None:intergenic | 35.0% | |
| AAGCAACCAATTGTTCTTCT+TGG | + | chr5.4:56773296-56773315 | None:intergenic | 35.0% | |
| AATGGAGCTTGAAGAAAGTT+TGG | - | chr5.4:56771178-56771197 | MS.gene26110:CDS | 35.0% | |
| ACAGATAAGGAGGGTAAATT+TGG | + | chr5.4:56770560-56770579 | None:intergenic | 35.0% | |
| ACTCATTTGGTATGAGACAT+AGG | - | chr5.4:56770442-56770461 | MS.gene26110:CDS | 35.0% | |
| AGAATTGAGTGCTTCACAAT+TGG | + | chr5.4:56772235-56772254 | None:intergenic | 35.0% | |
| AGATCATGATCATGATGAAG+AGG | - | chr5.4:56770695-56770714 | MS.gene26110:CDS | 35.0% | |
| ATAAACTTCCTGAAACTCCT+AGG | - | chr5.4:56772442-56772461 | MS.gene26110:intron | 35.0% | |
| ATCAGCTGAAAAAATTGAGG+AGG | - | chr5.4:56772368-56772387 | MS.gene26110:intron | 35.0% | |
| ATCCGACAATGACAGAAATT+TGG | - | chr5.4:56771585-56771604 | MS.gene26110:CDS | 35.0% | |
| CAATCTCCAAGAAGAACAAT+TGG | - | chr5.4:56773287-56773306 | MS.gene26110:CDS | 35.0% | |
| CAATGTGAAACCTGCATAAT+AGG | + | chr5.4:56770897-56770916 | None:intergenic | 35.0% | |
| CAGAAATTTGGAAGCAGATA+TGG | - | chr5.4:56771597-56771616 | MS.gene26110:CDS | 35.0% | |
| CCATCAATCATCAAAATGCT+CGG | - | chr5.4:56772398-56772417 | MS.gene26110:intron | 35.0% | |
| CGTTTGGATAAACATCTGAA+TGG | + | chr5.4:56771991-56772010 | None:intergenic | 35.0% | |
| CTTGTTCTTCCATCATTCTT+TGG | + | chr5.4:56772764-56772783 | None:intergenic | 35.0% | |
| GATCATTGTCCAAGTTCTTA+AGG | + | chr5.4:56773640-56773659 | None:intergenic | 35.0% | |
| GATGAGAGTTCATCAAGATA+TGG | - | chr5.4:56773221-56773240 | MS.gene26110:CDS | 35.0% | |
| GTTAGAAACGGTATCATCAT+TGG | + | chr5.4:56773051-56773070 | None:intergenic | 35.0% | |
| TAGTTTCCATCATACCAAGA+AGG | + | chr5.4:56770588-56770607 | None:intergenic | 35.0% | |
| TATCTCCTCCAAGTTAGAAA+CGG | + | chr5.4:56773063-56773082 | None:intergenic | 35.0% | |
| TGAATCATGCAAATCGAATG+AGG | - | chr5.4:56771031-56771050 | MS.gene26110:CDS | 35.0% | |
| TGAATTACGCCTTAAGAACT+TGG | - | chr5.4:56773628-56773647 | MS.gene26110:CDS | 35.0% | |
| TGATGATACCGTTTCTAACT+TGG | - | chr5.4:56773052-56773071 | MS.gene26110:CDS | 35.0% | |
| TGTATGGGATCCATACAAAA+TGG | - | chr5.4:56773542-56773561 | MS.gene26110:CDS | 35.0% | |
| TGTTTACCTTGTGCATTATC+TGG | + | chr5.4:56771302-56771321 | None:intergenic | 35.0% | |
| TGTTTCTCATGACTTAACCA+TGG | + | chr5.4:56770480-56770499 | None:intergenic | 35.0% | |
| TTTGGTATGAGACATAGGAT+TGG | - | chr5.4:56770447-56770466 | MS.gene26110:CDS | 35.0% | |
| ! | AGAAGGAATTGGAAGAGTAT+AGG | - | chr5.4:56772859-56772878 | MS.gene26110:CDS | 35.0% |
| ! | AGATCAACTCAAAGCATTAG+AGG | - | chr5.4:56773160-56773179 | MS.gene26110:CDS | 35.0% |
| ! | ATACAAAATGGTGGTGATGA+AGG | - | chr5.4:56773554-56773573 | MS.gene26110:CDS | 35.0% |
| ! | ATCTTCTTCAAGTGATAGTG+AGG | - | chr5.4:56772107-56772126 | MS.gene26110:intron | 35.0% |
| ! | CTCTTTGAGAAAACAGAATC+AGG | - | chr5.4:56772504-56772523 | MS.gene26110:CDS | 35.0% |
| ! | GAAAGTGTGTATTGAAGAAG+AGG | - | chr5.4:56773460-56773479 | MS.gene26110:CDS | 35.0% |
| ! | GAAGGAATTGGAAGAGTATA+GGG | - | chr5.4:56772860-56772879 | MS.gene26110:CDS | 35.0% |
| ! | TGCAACTTAACTATAGTGGA+TGG | + | chr5.4:56773662-56773681 | None:intergenic | 35.0% |
| ! | TTCATCACCACCATTTTGTA+TGG | + | chr5.4:56773555-56773574 | None:intergenic | 35.0% |
| !! | AAGTTTTATCTTCAGAGAGG+TGG | - | chr5.4:56770752-56770771 | MS.gene26110:CDS | 35.0% |
| AAGCTTGTGACGATGATGAT+CGG | - | chr5.4:56770964-56770983 | MS.gene26110:CDS | 40.0% | |
| AAGGCTTCAACAGATAAGGA+GGG | + | chr5.4:56770569-56770588 | None:intergenic | 40.0% | |
| AGAGAAGCAAGAACTTGAGA+AGG | - | chr5.4:56772842-56772861 | MS.gene26110:CDS | 40.0% | |
| ATGGGATCCATACAAAATGG+TGG | - | chr5.4:56773545-56773564 | MS.gene26110:CDS | 40.0% | |
| CAAATCGAATGAGGATCTAG+AGG | - | chr5.4:56771040-56771059 | MS.gene26110:CDS | 40.0% | |
| CAAGAAGGCTTCAACAGATA+AGG | + | chr5.4:56770573-56770592 | None:intergenic | 40.0% | |
| CTTATCTGTTGAAGCCTTCT+TGG | - | chr5.4:56770571-56770590 | MS.gene26110:CDS | 40.0% | |
| GAAGAACCAGATAATGCACA+AGG | - | chr5.4:56771293-56771312 | MS.gene26110:CDS | 40.0% | |
| GAGTGCTTCACAATTGGATA+TGG | + | chr5.4:56772229-56772248 | None:intergenic | 40.0% | |
| GCAAGAACTTGAGAAGGAAT+TGG | - | chr5.4:56772848-56772867 | MS.gene26110:CDS | 40.0% | |
| GGATGCAGAAAAAGATGAGA+AGG | - | chr5.4:56770821-56770840 | MS.gene26110:CDS | 40.0% | |
| GTATGGATCCCATACAATGT+TGG | + | chr5.4:56773538-56773557 | None:intergenic | 40.0% | |
| GTGAAAATTGCTTGGCTTCT+AGG | - | chr5.4:56770397-56770416 | MS.gene26110:CDS | 40.0% | |
| TCTTGTTCTTGTTGCAACAC+CGG | - | chr5.4:56772966-56772985 | MS.gene26110:CDS | 40.0% | |
| TGATACCGTTTCTAACTTGG+AGG | - | chr5.4:56773055-56773074 | MS.gene26110:CDS | 40.0% | |
| TGCACACAAGAAGATGAATC+TGG | - | chr5.4:56771478-56771497 | MS.gene26110:CDS | 40.0% | |
| TTCTGATGCTCCTATTATGC+AGG | - | chr5.4:56770884-56770903 | MS.gene26110:CDS | 40.0% | |
| TTGAAGCCTTCTTGGTATGA+TGG | - | chr5.4:56770579-56770598 | MS.gene26110:CDS | 40.0% | |
| TTGGTTAGTAGCTATTGCTG+AGG | + | chr5.4:56772676-56772695 | None:intergenic | 40.0% | |
| ! | AAATGGTGGTGATGAAGGAA+AGG | - | chr5.4:56773559-56773578 | MS.gene26110:CDS | 40.0% |
| ! | AGCTCTTTGTTCGGCTTTTA+GGG | + | chr5.4:56772613-56772632 | None:intergenic | 40.0% |
| ! | TGGATCAAGTGATGATGATG+AGG | - | chr5.4:56771498-56771517 | MS.gene26110:CDS | 40.0% |
| !! | ATCCGGTTTTAACAATCGAC+CGG | - | chr5.4:56772577-56772596 | MS.gene26110:CDS | 40.0% |
| !! | ATGCATTGACTTGAGTTGGT+TGG | + | chr5.4:56772075-56772094 | None:intergenic | 40.0% |
| !! | CCGAGCATTTTGATGATTGA+TGG | + | chr5.4:56772401-56772420 | None:intergenic | 40.0% |
| !! | CTTTTAGGGATGCTTTTAGC+CGG | + | chr5.4:56772599-56772618 | None:intergenic | 40.0% |
| !! | TCTTCTAAGACGCTTGATTG+CGG | + | chr5.4:56771113-56771132 | None:intergenic | 40.0% |
| !! | TGACATGCATTGACTTGAGT+TGG | + | chr5.4:56772079-56772098 | None:intergenic | 40.0% |
| !! | TGCATTATCTGGTTCTTCTG+TGG | + | chr5.4:56771291-56771310 | None:intergenic | 40.0% |
| AACTGAACTCGATCGATCCT+CGG | + | chr5.4:56770778-56770797 | None:intergenic | 45.0% | |
| AGCCGGTCGATTGTTAAAAC+CGG | + | chr5.4:56772582-56772601 | None:intergenic | 45.0% | |
| ATCGAATGAGGATCTAGAGG+AGG | - | chr5.4:56771043-56771062 | MS.gene26110:CDS | 45.0% | |
| ATGCAATGCACTACATGCAG+AGG | - | chr5.4:56772472-56772491 | MS.gene26110:CDS | 45.0% | |
| GAACAAGAAAATGCTCCACC+AGG | - | chr5.4:56771622-56771641 | MS.gene26110:CDS | 45.0% | |
| GAAGGCTTCAACAGATAAGG+AGG | + | chr5.4:56770570-56770589 | None:intergenic | 45.0% | |
| GCAATCAAGCGTCTTAGAAG+AGG | - | chr5.4:56771112-56771131 | MS.gene26110:CDS | 45.0% | |
| TACAAGGCTGCAGGAAGAAA+AGG | - | chr5.4:56772710-56772729 | MS.gene26110:intron | 45.0% | |
| TCGAATGAGGATCTAGAGGA+GGG | - | chr5.4:56771044-56771063 | MS.gene26110:CDS | 45.0% | |
| TGAGGTAGAATACAGCGATC+CGG | - | chr5.4:56772560-56772579 | MS.gene26110:CDS | 45.0% | |
| ! | AGCACTGAGAGCTCTTTGTT+CGG | + | chr5.4:56772622-56772641 | None:intergenic | 45.0% |
| ! | AGGTTGATCGCGTTTATGAC+AGG | - | chr5.4:56773480-56773499 | MS.gene26110:CDS | 45.0% |
| ! | GAGCTCTTTGTTCGGCTTTT+AGG | + | chr5.4:56772614-56772633 | None:intergenic | 45.0% |
| ! | GAGTTGGTTGGTTCTTCAGA+TGG | + | chr5.4:56772063-56772082 | None:intergenic | 45.0% |
| ! | TTGGTTGGTTCTTCAGATGG+AGG | + | chr5.4:56772060-56772079 | None:intergenic | 45.0% |
| AAGGCTGCAGGAAGAAAAGG+CGG | - | chr5.4:56772713-56772732 | MS.gene26110:intron | 50.0% | |
| ACGCTTGATTGCGGTTCAAC+TGG | + | chr5.4:56771104-56771123 | None:intergenic | 50.0% | |
| ACTTGCAACGCTACCATCCA+CGG | + | chr5.4:56772541-56772560 | None:intergenic | 50.0% | |
| AGAAAAGGCGGCAATGCAGA+TGG | - | chr5.4:56772725-56772744 | MS.gene26110:intron | 50.0% | |
| ATCAGGAGCAGAAGAATCCG+TGG | - | chr5.4:56772521-56772540 | MS.gene26110:CDS | 50.0% | |
| CCAGGTACAAAGACAGTGCA+TGG | - | chr5.4:56771640-56771659 | MS.gene26110:CDS | 50.0% | |
| CCATGCACTGTCTTTGTACC+TGG | + | chr5.4:56771643-56771662 | None:intergenic | 50.0% | |
| CGAATGAGGATCTAGAGGAG+GGG | - | chr5.4:56771045-56771064 | MS.gene26110:CDS | 50.0% | |
| GGCAATGATTACAAGGCTGC+AGG | - | chr5.4:56772701-56772720 | MS.gene26110:intron | 50.0% | |
| TCTGCTGACCTAGGAGTTTC+AGG | + | chr5.4:56772453-56772472 | None:intergenic | 50.0% | |
| TGACAGTTCATCGGTGTAGC+CGG | + | chr5.4:56772988-56773007 | None:intergenic | 50.0% | |
| TGCACTGTCTTTGTACCTGG+TGG | + | chr5.4:56771640-56771659 | None:intergenic | 50.0% | |
| TGCATTGCATCTGCTGACCT+AGG | + | chr5.4:56772462-56772481 | None:intergenic | 50.0% | |
| GGAGCAGAAGAATCCGTGGA+TGG | - | chr5.4:56772525-56772544 | MS.gene26110:CDS | 55.0% | |
| TATCTTCAGAGAGGTGGCCG+AGG | - | chr5.4:56770758-56770777 | MS.gene26110:CDS | 55.0% | |
| TGAGGATCTAGAGGAGGGGA+AGG | - | chr5.4:56771049-56771068 | MS.gene26110:CDS | 55.0% | |
| ! | GGATGGTAGCGTTGCAAGTG+AGG | - | chr5.4:56772542-56772561 | MS.gene26110:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.4 | gene | 56770393 | 56773703 | 56770393 | ID=MS.gene26110 |
| chr5.4 | mRNA | 56770393 | 56773703 | 56770393 | ID=MS.gene26110.t1;Parent=MS.gene26110 |
| chr5.4 | exon | 56772782 | 56773703 | 56772782 | ID=MS.gene26110.t1.exon1;Parent=MS.gene26110.t1 |
| chr5.4 | CDS | 56772782 | 56773703 | 56772782 | ID=cds.MS.gene26110.t1;Parent=MS.gene26110.t1 |
| chr5.4 | exon | 56772453 | 56772659 | 56772453 | ID=MS.gene26110.t1.exon2;Parent=MS.gene26110.t1 |
| chr5.4 | CDS | 56772453 | 56772659 | 56772453 | ID=cds.MS.gene26110.t1;Parent=MS.gene26110.t1 |
| chr5.4 | exon | 56771913 | 56772047 | 56771913 | ID=MS.gene26110.t1.exon3;Parent=MS.gene26110.t1 |
| chr5.4 | CDS | 56771913 | 56772047 | 56771913 | ID=cds.MS.gene26110.t1;Parent=MS.gene26110.t1 |
| chr5.4 | exon | 56770393 | 56771777 | 56770393 | ID=MS.gene26110.t1.exon4;Parent=MS.gene26110.t1 |
| chr5.4 | CDS | 56770393 | 56771777 | 56770393 | ID=cds.MS.gene26110.t1;Parent=MS.gene26110.t1 |
| Gene Sequence |
| Protein sequence |
