Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene059426.t1 | XP_003607925.1 | 95.2 | 124 | 6 | 0 | 1 | 124 | 306 | 429 | 2.00E-55 | 224.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene059426.t1 | Q9SLN1 | 38.2 | 123 | 75 | 1 | 1 | 123 | 299 | 420 | 2.0e-11 | 70.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene059426.t1 | G7JK12 | 95.2 | 124 | 6 | 0 | 1 | 124 | 306 | 429 | 1.5e-55 | 224.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049350 | MS.gene059426 | 0.80246 | 5.55E-49 | -1.69E-46 |
| MS.gene053144 | MS.gene059426 | 0.81158 | 6.52E-51 | -1.69E-46 |
| MS.gene053536 | MS.gene059426 | 0.837801 | 4.19E-57 | -1.69E-46 |
| MS.gene053538 | MS.gene059426 | 0.836541 | 8.81E-57 | -1.69E-46 |
| MS.gene054584 | MS.gene059426 | 0.806905 | 6.56E-50 | -1.69E-46 |
| MS.gene055465 | MS.gene059426 | 0.817265 | 3.61E-52 | -1.69E-46 |
| MS.gene055469 | MS.gene059426 | 0.813743 | 2.19E-51 | -1.69E-46 |
| MS.gene055537 | MS.gene059426 | 0.815638 | 8.34E-52 | -1.69E-46 |
| MS.gene055764 | MS.gene059426 | 0.80549 | 1.30E-49 | -1.69E-46 |
| MS.gene058087 | MS.gene059426 | 0.807117 | 5.91E-50 | -1.69E-46 |
| MS.gene059424 | MS.gene059426 | 0.829769 | 4.28E-55 | -1.69E-46 |
| MS.gene059426 | MS.gene062184 | 0.816386 | 5.68E-52 | -1.69E-46 |
| MS.gene059426 | MS.gene06258 | 0.826346 | 2.85E-54 | -1.69E-46 |
| MS.gene059426 | MS.gene064161 | 0.816448 | 5.50E-52 | -1.69E-46 |
| MS.gene059426 | MS.gene064520 | 0.834685 | 2.60E-56 | -1.69E-46 |
| MS.gene059426 | MS.gene064521 | 0.843464 | 1.38E-58 | -1.69E-46 |
| MS.gene059426 | MS.gene064522 | 0.828922 | 6.87E-55 | -1.69E-46 |
| MS.gene059426 | MS.gene070339 | 0.844042 | 9.65E-59 | -1.69E-46 |
| MS.gene059426 | MS.gene073083 | 0.80382 | 2.91E-49 | -1.69E-46 |
| MS.gene059426 | MS.gene073262 | 0.806733 | 7.13E-50 | -1.69E-46 |
| MS.gene059426 | MS.gene074519 | 0.810145 | 1.33E-50 | -1.69E-46 |
| MS.gene059426 | MS.gene07486 | 0.808078 | 3.69E-50 | -1.69E-46 |
| MS.gene059426 | MS.gene07723 | 0.805021 | 1.63E-49 | -1.69E-46 |
| MS.gene059426 | MS.gene21826 | 0.812818 | 3.50E-51 | -1.69E-46 |
| MS.gene059426 | MS.gene21960 | 0.803411 | 3.53E-49 | -1.69E-46 |
| MS.gene059426 | MS.gene26110 | 0.835385 | 1.73E-56 | -1.69E-46 |
| MS.gene059426 | MS.gene26380 | 0.821222 | 4.53E-53 | -1.69E-46 |
| MS.gene059426 | MS.gene26387 | 0.806853 | 6.72E-50 | -1.69E-46 |
| MS.gene059426 | MS.gene26464 | 0.829176 | 5.96E-55 | -1.69E-46 |
| MS.gene059426 | MS.gene26465 | 0.845465 | 3.99E-59 | -1.69E-46 |
| MS.gene059426 | MS.gene26659 | 0.81222 | 4.73E-51 | -1.69E-46 |
| MS.gene059426 | MS.gene26801 | 0.806011 | 1.01E-49 | -1.69E-46 |
| MS.gene059426 | MS.gene26851 | 0.819832 | 9.44E-53 | -1.69E-46 |
| MS.gene059426 | MS.gene26855 | 0.804565 | 2.03E-49 | -1.69E-46 |
| MS.gene059426 | MS.gene27350 | 0.814809 | 1.28E-51 | -1.69E-46 |
| MS.gene059426 | MS.gene31433 | 0.806121 | 9.59E-50 | -1.69E-46 |
| MS.gene059426 | MS.gene34286 | 0.813267 | 2.79E-51 | -1.69E-46 |
| MS.gene059426 | MS.gene36265 | 0.806475 | 8.08E-50 | -1.69E-46 |
| MS.gene059426 | MS.gene38303 | 0.818902 | 1.54E-52 | -1.69E-46 |
| MS.gene059426 | MS.gene38829 | 0.809975 | 1.45E-50 | -1.69E-46 |
| MS.gene059426 | MS.gene39046 | 0.800197 | 1.61E-48 | -1.69E-46 |
| MS.gene059426 | MS.gene39523 | 0.810956 | 8.91E-51 | -1.69E-46 |
| MS.gene059426 | MS.gene41651 | 0.817385 | 3.39E-52 | -1.69E-46 |
| MS.gene059426 | MS.gene43075 | 0.806891 | 6.60E-50 | -1.69E-46 |
| MS.gene059426 | MS.gene45425 | 0.806647 | 7.43E-50 | -1.69E-46 |
| MS.gene059426 | MS.gene47586 | 0.800779 | 1.23E-48 | -1.69E-46 |
| MS.gene059426 | MS.gene48347 | 0.804485 | 2.11E-49 | -1.69E-46 |
| MS.gene059426 | MS.gene56851 | 0.801144 | 1.03E-48 | -1.69E-46 |
| MS.gene059426 | MS.gene58836 | 0.813259 | 2.80E-51 | -1.69E-46 |
| MS.gene059426 | MS.gene62806 | 0.800095 | 1.69E-48 | -1.69E-46 |
| MS.gene059426 | MS.gene62916 | 0.810717 | 1.00E-50 | -1.69E-46 |
| MS.gene059426 | MS.gene68106 | 0.803917 | 2.77E-49 | -1.69E-46 |
| MS.gene059426 | MS.gene68107 | 0.807881 | 4.07E-50 | -1.69E-46 |
| MS.gene059426 | MS.gene68418 | 0.81358 | 2.38E-51 | -1.69E-46 |
| MS.gene059426 | MS.gene76518 | 0.802876 | 4.56E-49 | -1.69E-46 |
| MS.gene059426 | MS.gene78530 | 0.848957 | 4.40E-60 | -1.69E-46 |
| MS.gene059426 | MS.gene85133 | 0.800525 | 1.38E-48 | -1.69E-46 |
| MS.gene059426 | MS.gene86799 | 0.805736 | 1.16E-49 | -1.69E-46 |
| MS.gene059426 | MS.gene90646 | 0.812381 | 4.36E-51 | -1.69E-46 |
| MS.gene059426 | MS.gene95401 | 0.804733 | 1.87E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene059426.t1 | MTR_4g085570 | 95.161 | 124 | 6 | 0 | 1 | 124 | 306 | 429 | 7.40e-75 | 238 |
| MS.gene059426.t1 | MTR_4g085570 | 95.161 | 124 | 6 | 0 | 1 | 124 | 306 | 429 | 1.93e-74 | 238 |
| MS.gene059426.t1 | MTR_4g094848 | 42.276 | 123 | 69 | 1 | 1 | 123 | 212 | 332 | 1.01e-14 | 69.7 |
| MS.gene059426.t1 | MTR_4g094848 | 42.276 | 123 | 69 | 1 | 1 | 123 | 324 | 444 | 1.05e-14 | 69.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene059426.t1 | AT2G23360 | 40.000 | 125 | 70 | 3 | 1 | 123 | 299 | 420 | 4.96e-14 | 67.8 |
Find 23 sgRNAs with CRISPR-Local
Find 24 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GATGATGAGGCTATCTCTTC+TGG | 0.311197 | 4.3:-27227261 | MS.gene059426:CDS |
| CTGGGCAAATGCTCTTATTT+CGG | 0.322833 | 4.3:-27227235 | MS.gene059426:CDS |
| GTACATGTCCTGAGCTTCTA+TGG | 0.340264 | 4.3:+27227161 | None:intergenic |
| AGGCTATCTCTTCTGGATCC+TGG | 0.342477 | 4.3:-27227254 | MS.gene059426:CDS |
| TGTTTCGGAGGCTAAAATTC+AGG | 0.351476 | 4.3:-27227196 | MS.gene059426:CDS |
| ACTTGAACATTTACGTGTTT+CGG | 0.357826 | 4.3:-27227211 | MS.gene059426:CDS |
| AGCTCAGGACATGTACTCTA+TGG | 0.392566 | 4.3:-27227154 | MS.gene059426:CDS |
| ATGTCAAAATTTGACATTGA+AGG | 0.437532 | 4.3:+27227291 | None:intergenic |
| GGATTCTTCAAGATTATGTA+TGG | 0.449330 | 4.3:-27227420 | MS.gene059426:CDS |
| GAAGGAAGTTCATTTGATGT+AGG | 0.454421 | 4.3:+27227309 | None:intergenic |
| GGCTATCTCTTCTGGATCCT+GGG | 0.459636 | 4.3:-27227253 | MS.gene059426:CDS |
| CAATAAAGCCATAGAAGCTC+AGG | 0.475405 | 4.3:-27227169 | MS.gene059426:CDS |
| TTCTGAAAATTACAAGTCCA+TGG | 0.477001 | 4.3:-27227349 | MS.gene059426:CDS |
| AATCTCAGCCTGTGACAACC+TGG | 0.488184 | 4.3:+27227386 | None:intergenic |
| AACAGCATCCAGGTTGTCAC+AGG | 0.541203 | 4.3:-27227394 | MS.gene059426:CDS |
| TGACATTTCTAGTGATGATG+AGG | 0.554232 | 4.3:-27227274 | MS.gene059426:CDS |
| ACAAGTCCATGGAGCTAGCA+AGG | 0.566040 | 4.3:-27227338 | MS.gene059426:CDS |
| ACATATACAGGATATGGATG+AGG | 0.569404 | 4.3:-27227490 | None:intergenic |
| ATAACACCTTGCTAGCTCCA+TGG | 0.573593 | 4.3:+27227332 | None:intergenic |
| TGTATGGTGAAACAGCATCC+AGG | 0.582574 | 4.3:-27227404 | MS.gene059426:CDS |
| CGAAATAAGAGCATTTGCCC+AGG | 0.583249 | 4.3:+27227236 | None:intergenic |
| TGAACATTTACGTGTTTCGG+AGG | 0.617084 | 4.3:-27227208 | MS.gene059426:CDS |
| GGATATGGATGAGGAAAACA+AGG | 0.627279 | 4.3:-27227481 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | ATGTCAAAATTTGACATTGA+AGG | + | chr4.3:27227311-27227330 | None:intergenic | 25.0% |
| ACTTGAACATTTACGTGTTT+CGG | - | chr4.3:27227388-27227407 | MS.gene059426:CDS | 30.0% | |
| GGATTCTTCAAGATTATGTA+TGG | - | chr4.3:27227179-27227198 | MS.gene059426:CDS | 30.0% | |
| ! | ATTTTGTTGAGATGGAGAAA+AGG | - | chr4.3:27227471-27227490 | MS.gene059426:CDS | 30.0% |
| ! | TATGGATGATTTTGTTGAGA+TGG | - | chr4.3:27227463-27227482 | MS.gene059426:CDS | 30.0% |
| ! | TTCTGAAAATTACAAGTCCA+TGG | - | chr4.3:27227250-27227269 | MS.gene059426:CDS | 30.0% |
| AACGAGAAAAACTCTGAACT+AGG | - | chr4.3:27227158-27227177 | MS.gene059426:CDS | 35.0% | |
| GAAGGAAGTTCATTTGATGT+AGG | + | chr4.3:27227293-27227312 | None:intergenic | 35.0% | |
| !! | TGACATTTCTAGTGATGATG+AGG | - | chr4.3:27227325-27227344 | MS.gene059426:CDS | 35.0% |
| CAATAAAGCCATAGAAGCTC+AGG | - | chr4.3:27227430-27227449 | MS.gene059426:CDS | 40.0% | |
| TGAACATTTACGTGTTTCGG+AGG | - | chr4.3:27227391-27227410 | MS.gene059426:CDS | 40.0% | |
| TGTTTCGGAGGCTAAAATTC+AGG | - | chr4.3:27227403-27227422 | MS.gene059426:CDS | 40.0% | |
| ! | CTGGGCAAATGCTCTTATTT+CGG | - | chr4.3:27227364-27227383 | MS.gene059426:CDS | 40.0% |
| AGCTCAGGACATGTACTCTA+TGG | - | chr4.3:27227445-27227464 | MS.gene059426:CDS | 45.0% | |
| ATAACACCTTGCTAGCTCCA+TGG | + | chr4.3:27227270-27227289 | None:intergenic | 45.0% | |
| GATGATGAGGCTATCTCTTC+TGG | - | chr4.3:27227338-27227357 | MS.gene059426:CDS | 45.0% | |
| GTACATGTCCTGAGCTTCTA+TGG | + | chr4.3:27227441-27227460 | None:intergenic | 45.0% | |
| TGTATGGTGAAACAGCATCC+AGG | - | chr4.3:27227195-27227214 | MS.gene059426:CDS | 45.0% | |
| !! | CGAAATAAGAGCATTTGCCC+AGG | + | chr4.3:27227366-27227385 | None:intergenic | 45.0% |
| AACAGCATCCAGGTTGTCAC+AGG | - | chr4.3:27227205-27227224 | MS.gene059426:CDS | 50.0% | |
| AATCTCAGCCTGTGACAACC+TGG | + | chr4.3:27227216-27227235 | None:intergenic | 50.0% | |
| ACAAGTCCATGGAGCTAGCA+AGG | - | chr4.3:27227261-27227280 | MS.gene059426:CDS | 50.0% | |
| AGGCTATCTCTTCTGGATCC+TGG | - | chr4.3:27227345-27227364 | MS.gene059426:CDS | 50.0% | |
| GGCTATCTCTTCTGGATCCT+GGG | - | chr4.3:27227346-27227365 | MS.gene059426:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.3 | gene | 27227122 | 27227499 | 27227122 | ID=MS.gene059426 |
| chr4.3 | mRNA | 27227122 | 27227499 | 27227122 | ID=MS.gene059426.t1;Parent=MS.gene059426 |
| chr4.3 | exon | 27227122 | 27227499 | 27227122 | ID=MS.gene059426.t1.exon1;Parent=MS.gene059426.t1 |
| chr4.3 | CDS | 27227122 | 27227499 | 27227122 | ID=cds.MS.gene059426.t1;Parent=MS.gene059426.t1 |
| Gene Sequence |
| Protein sequence |
