Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene26160.t1 | RHN60700.1 | 99.4 | 178 | 1 | 0 | 1 | 178 | 4 | 181 | 1.50E-51 | 212.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene26160.t1 | Q9H6L4 | 62.1 | 58 | 22 | 0 | 19 | 76 | 13 | 70 | 1.8e-13 | 77.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene26160.t1 | A0A396I501 | 99.4 | 178 | 1 | 0 | 1 | 178 | 4 | 181 | 1.1e-51 | 212.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene26160 | MS.gene044085 | PPI |
| MS.gene26160 | MS.gene24450 | PPI |
| MS.gene00474 | MS.gene26160 | PPI |
| MS.gene00478 | MS.gene26160 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene26160.t1 | MTR_5g077040 | 98.315 | 178 | 3 | 0 | 1 | 178 | 1 | 178 | 4.65e-128 | 357 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene26160.t1 | AT5G37290 | 72.067 | 179 | 48 | 1 | 1 | 177 | 1 | 179 | 2.87e-99 | 284 |
| MS.gene26160.t1 | AT5G37290 | 72.067 | 179 | 48 | 1 | 1 | 177 | 1 | 179 | 2.87e-99 | 284 |
| MS.gene26160.t1 | AT5G37290 | 71.311 | 122 | 35 | 0 | 1 | 122 | 1 | 122 | 1.00e-65 | 197 |
Find 42 sgRNAs with CRISPR-Local
Find 69 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTCTTGTTGCCTTTGGTTAT+TGG | 0.174361 | 5.4:+57349102 | None:intergenic |
| AATGAAAAGATTATAGAATT+TGG | 0.211644 | 5.4:-57348606 | MS.gene26160:CDS |
| AGAAATTAAAGAGAAGATTT+TGG | 0.229064 | 5.4:-57348800 | MS.gene26160:CDS |
| ATAATTGCAGCTAAATGTTT+TGG | 0.267268 | 5.4:-57348658 | MS.gene26160:intron |
| ATCCTTACAGTGTTCCTAAC+TGG | 0.299126 | 5.4:+57348393 | None:intergenic |
| CGGTCCGCTCTTGTTGCCTT+TGG | 0.333981 | 5.4:+57349095 | None:intergenic |
| GTTAAAATTGTAAGGATCAT+AGG | 0.342362 | 5.4:+57348759 | None:intergenic |
| TAGTGTGAGCTTCAGTAATC+TGG | 0.391697 | 5.4:-57348014 | MS.gene26160:CDS |
| CAAGAGCGGACCGGAAGATA+TGG | 0.397319 | 5.4:-57349085 | MS.gene26160:CDS |
| CACCTGAAGGTATTGCAATC+TGG | 0.406154 | 5.4:+57349057 | None:intergenic |
| AATGTTTATCAAGCCCAGTT+AGG | 0.407380 | 5.4:-57348407 | MS.gene26160:CDS |
| CGCAAGAAGTTAAAATTGTA+AGG | 0.419936 | 5.4:+57348751 | None:intergenic |
| CCTTTGGTTATTGGTGAACA+TGG | 0.442442 | 5.4:+57349111 | None:intergenic |
| TTGATGACATCAATAACTTC+AGG | 0.448267 | 5.4:+57348061 | None:intergenic |
| TTCCAGATTGCAATACCTTC+AGG | 0.449232 | 5.4:-57349059 | MS.gene26160:intron |
| GATAAACATTGAATGATAAG+AGG | 0.452418 | 5.4:+57348420 | None:intergenic |
| ATTATAGAATTTGGTGTTGG+AGG | 0.454107 | 5.4:-57348597 | MS.gene26160:CDS |
| TCCTTACAGTGTTCCTAACT+GGG | 0.468459 | 5.4:+57348394 | None:intergenic |
| AAGATTATAGAATTTGGTGT+TGG | 0.481352 | 5.4:-57348600 | MS.gene26160:CDS |
| TTCTTGACAAACATCTATCA+AGG | 0.487090 | 5.4:-57347982 | MS.gene26160:CDS |
| TTATAGAATTTGGTGTTGGA+GGG | 0.493970 | 5.4:-57348596 | MS.gene26160:CDS |
| GCTACAATTGTAGTGTTGGC+TGG | 0.501124 | 5.4:+57348459 | None:intergenic |
| TCTGGAAGTACCATATCTTC+CGG | 0.518139 | 5.4:+57349075 | None:intergenic |
| GCCAACACTACAATTGTAGC+TGG | 0.525116 | 5.4:-57348456 | MS.gene26160:CDS |
| ACAATTGTAGCTGGAGTTGG+TGG | 0.530269 | 5.4:-57348447 | MS.gene26160:CDS |
| GTAAGGATCATAGGCAAAGT+TGG | 0.532617 | 5.4:+57348768 | None:intergenic |
| ATCAGGTAAATTATGCACTC+GGG | 0.532999 | 5.4:-57348132 | MS.gene26160:intron |
| TCCAGCTACAATTGTAGTGT+TGG | 0.542442 | 5.4:+57348455 | None:intergenic |
| CAAAGGCAACAAGAGCGGAC+CGG | 0.544877 | 5.4:-57349094 | MS.gene26160:CDS |
| GATCAGGTAAATTATGCACT+CGG | 0.549275 | 5.4:-57348133 | MS.gene26160:intron |
| TAATGAATCTAACAAGGAAG+AGG | 0.565289 | 5.4:-57348092 | MS.gene26160:CDS |
| GGGATCTGCAATTGTTGTGT+TGG | 0.566984 | 5.4:-57348576 | MS.gene26160:intron |
| TGGGTTCAGTTACGCAGTCA+AGG | 0.570036 | 5.4:+57348628 | None:intergenic |
| ACTACAATTGTAGCTGGAGT+TGG | 0.573756 | 5.4:-57348450 | MS.gene26160:CDS |
| TTTCAGAATTCATCAGAGGA+TGG | 0.593173 | 5.4:-57348940 | MS.gene26160:intron |
| CATCTGTAATGAATCTAACA+AGG | 0.607086 | 5.4:-57348098 | MS.gene26160:CDS |
| CAATTGTAGCTGGAGTTGGT+GGG | 0.628633 | 5.4:-57348446 | MS.gene26160:CDS |
| AAGTTATTGATGTCATCAAG+AGG | 0.636725 | 5.4:-57348057 | MS.gene26160:CDS |
| TCAGTTTCAGAATTCATCAG+AGG | 0.641335 | 5.4:-57348944 | MS.gene26160:CDS |
| CCATGTTCACCAATAACCAA+AGG | 0.659452 | 5.4:-57349111 | MS.gene26160:CDS |
| ATAACCAAAGGCAACAAGAG+CGG | 0.667897 | 5.4:-57349099 | MS.gene26160:CDS |
| TCAGGTAAATTATGCACTCG+GGG | 0.677160 | 5.4:-57348131 | MS.gene26160:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATGAAAAGATTATAGAATT+TGG | - | chr5.4:57348476-57348495 | MS.gene26160:intron | 15.0% |
| !!! | AAATTCTATAATCTTTTCAT+TGG | + | chr5.4:57348477-57348496 | None:intergenic | 15.0% |
| !!! | AATTCTATAATCTTTTCATT+GGG | + | chr5.4:57348476-57348495 | None:intergenic | 15.0% |
| !!! | AGTTGTTTATCAAATTTTTA+GGG | + | chr5.4:57348916-57348935 | None:intergenic | 15.0% |
| !!! | TAGTTGTTTATCAAATTTTT+AGG | + | chr5.4:57348917-57348936 | None:intergenic | 15.0% |
| !! | ATGCAAATTAAGTAGTATAA+GGG | + | chr5.4:57348788-57348807 | None:intergenic | 20.0% |
| !!! | AGAAATTAAAGAGAAGATTT+TGG | - | chr5.4:57348282-57348301 | MS.gene26160:intron | 20.0% |
| !!! | GTTGTTTATCAAATTTTTAG+GGG | + | chr5.4:57348915-57348934 | None:intergenic | 20.0% |
| ! | ACTAATGTATTTATCTGATC+AGG | - | chr5.4:57348933-57348952 | MS.gene26160:intron | 25.0% |
| ! | GAAACAACGTTATTCATATA+AGG | + | chr5.4:57348105-57348124 | None:intergenic | 25.0% |
| ! | GATAAACATTGAATGATAAG+AGG | + | chr5.4:57348665-57348684 | None:intergenic | 25.0% |
| ! | GATGCAAATTAAGTAGTATA+AGG | + | chr5.4:57348789-57348808 | None:intergenic | 25.0% |
| ! | GTTAAAATTGTAAGGATCAT+AGG | + | chr5.4:57348326-57348345 | None:intergenic | 25.0% |
| ! | TTATTGCAAAGACAATTAGT+GGG | + | chr5.4:57348368-57348387 | None:intergenic | 25.0% |
| !! | ATAATTGCAGCTAAATGTTT+TGG | - | chr5.4:57348424-57348443 | MS.gene26160:CDS | 25.0% |
| !! | TATATGAATAACGTTGTTTC+AGG | - | chr5.4:57348105-57348124 | MS.gene26160:CDS | 25.0% |
| !!! | AAGATTATAGAATTTGGTGT+TGG | - | chr5.4:57348482-57348501 | MS.gene26160:intron | 25.0% |
| AAGTTATTGATGTCATCAAG+AGG | - | chr5.4:57349025-57349044 | MS.gene26160:intron | 30.0% | |
| AGAAATCACAATACTGTGAA+AGG | + | chr5.4:57348549-57348568 | None:intergenic | 30.0% | |
| ATAAAGAATCCTACCAACAA+AGG | + | chr5.4:57348217-57348236 | None:intergenic | 30.0% | |
| CATCTGTAATGAATCTAACA+AGG | - | chr5.4:57348984-57349003 | MS.gene26160:intron | 30.0% | |
| CGCAAGAAGTTAAAATTGTA+AGG | + | chr5.4:57348334-57348353 | None:intergenic | 30.0% | |
| CTTATTGCAAAGACAATTAG+TGG | + | chr5.4:57348369-57348388 | None:intergenic | 30.0% | |
| TAATGAATCTAACAAGGAAG+AGG | - | chr5.4:57348990-57349009 | MS.gene26160:intron | 30.0% | |
| TTCTTGACAAACATCTATCA+AGG | - | chr5.4:57349100-57349119 | MS.gene26160:CDS | 30.0% | |
| ! | AATAACGTTGTTTCAGGAAT+TGG | - | chr5.4:57348111-57348130 | MS.gene26160:CDS | 30.0% |
| ! | TTGATGACATCAATAACTTC+AGG | + | chr5.4:57349024-57349043 | None:intergenic | 30.0% |
| ! | TTTACCATCTCCTTATTTGT+TGG | - | chr5.4:57348238-57348257 | MS.gene26160:intron | 30.0% |
| !! | AATTTTTAGGGGATCTTTTG+TGG | + | chr5.4:57348904-57348923 | None:intergenic | 30.0% |
| !! | AGAGAAGATTTTGGCAAATT+TGG | - | chr5.4:57348291-57348310 | MS.gene26160:intron | 30.0% |
| !! | ATTATAGAATTTGGTGTTGG+AGG | - | chr5.4:57348485-57348504 | MS.gene26160:intron | 30.0% |
| !! | TTATAGAATTTGGTGTTGGA+GGG | - | chr5.4:57348486-57348505 | MS.gene26160:intron | 30.0% |
| !!! | GTTTGCTTGTTGATTTTGTA+AGG | - | chr5.4:57348716-57348735 | MS.gene26160:intron | 30.0% |
| AACACCAACAAATAAGGAGA+TGG | + | chr5.4:57348245-57348264 | None:intergenic | 35.0% | |
| AATCTTTCAAGCACCTTTGT+TGG | - | chr5.4:57348201-57348220 | MS.gene26160:intron | 35.0% | |
| AATGTTTATCAAGCCCAGTT+AGG | - | chr5.4:57348675-57348694 | MS.gene26160:intron | 35.0% | |
| ACGAGAAACACCAACAAATA+AGG | + | chr5.4:57348251-57348270 | None:intergenic | 35.0% | |
| ATCAGGTAAATTATGCACTC+GGG | - | chr5.4:57348950-57348969 | MS.gene26160:CDS | 35.0% | |
| GAAGAAGAAAAAACACCTGA+AGG | + | chr5.4:57348041-57348060 | None:intergenic | 35.0% | |
| GATCAGGTAAATTATGCACT+CGG | - | chr5.4:57348949-57348968 | MS.gene26160:CDS | 35.0% | |
| TCAGTTTCAGAATTCATCAG+AGG | - | chr5.4:57348138-57348157 | MS.gene26160:intron | 35.0% | |
| TTTCAGAATTCATCAGAGGA+TGG | - | chr5.4:57348142-57348161 | MS.gene26160:intron | 35.0% | |
| !! | ACAAGCGATTTTGAGAAATC+AGG | + | chr5.4:57348819-57348838 | None:intergenic | 35.0% |
| ACTACAATTGTAGCTGGAGT+TGG | - | chr5.4:57348632-57348651 | MS.gene26160:CDS | 40.0% | |
| ATAACCAAAGGCAACAAGAG+CGG | - | chr5.4:57347983-57348002 | MS.gene26160:CDS | 40.0% | |
| ATCCTTACAGTGTTCCTAAC+TGG | + | chr5.4:57348692-57348711 | None:intergenic | 40.0% | |
| CTCTTGTTGCCTTTGGTTAT+TGG | + | chr5.4:57347983-57348002 | None:intergenic | 40.0% | |
| GTAAGGATCATAGGCAAAGT+TGG | + | chr5.4:57348317-57348336 | None:intergenic | 40.0% | |
| TAGTGTGAGCTTCAGTAATC+TGG | - | chr5.4:57349068-57349087 | MS.gene26160:CDS | 40.0% | |
| TCAGGTAAATTATGCACTCG+GGG | - | chr5.4:57348951-57348970 | MS.gene26160:CDS | 40.0% | |
| TCCAGCTACAATTGTAGTGT+TGG | + | chr5.4:57348630-57348649 | None:intergenic | 40.0% | |
| TCCTTACAGTGTTCCTAACT+GGG | + | chr5.4:57348691-57348710 | None:intergenic | 40.0% | |
| TTCCAGATTGCAATACCTTC+AGG | - | chr5.4:57348023-57348042 | MS.gene26160:CDS | 40.0% | |
| TTTCAAGCACCTTTGTTGGT+AGG | - | chr5.4:57348205-57348224 | MS.gene26160:intron | 40.0% | |
| !! | TCTGGAAGTACCATATCTTC+CGG | + | chr5.4:57348010-57348029 | None:intergenic | 40.0% |
| !!! | CAATTTTAACTTCTTGCGCC+AGG | - | chr5.4:57348336-57348355 | MS.gene26160:intron | 40.0% |
| ACAATTGTAGCTGGAGTTGG+TGG | - | chr5.4:57348635-57348654 | MS.gene26160:CDS | 45.0% | |
| GCCAACACTACAATTGTAGC+TGG | - | chr5.4:57348626-57348645 | MS.gene26160:CDS | 45.0% | |
| GGGATCTGCAATTGTTGTGT+TGG | - | chr5.4:57348506-57348525 | MS.gene26160:intron | 45.0% | |
| TTAACTTCTTGCGCCAGGTA+TGG | - | chr5.4:57348341-57348360 | MS.gene26160:intron | 45.0% | |
| ! | ACAATTAGTGGGTCCATACC+TGG | + | chr5.4:57348357-57348376 | None:intergenic | 45.0% |
| ! | CAATTGTAGCTGGAGTTGGT+GGG | - | chr5.4:57348636-57348655 | MS.gene26160:CDS | 45.0% |
| !! | CACCTGAAGGTATTGCAATC+TGG | + | chr5.4:57348028-57348047 | None:intergenic | 45.0% |
| !! | GCTACAATTGTAGTGTTGGC+TGG | + | chr5.4:57348626-57348645 | None:intergenic | 45.0% |
| GCCCAGTTAGGAACACTGTA+AGG | - | chr5.4:57348687-57348706 | MS.gene26160:intron | 50.0% | |
| TGGGTTCAGTTACGCAGTCA+AGG | + | chr5.4:57348457-57348476 | None:intergenic | 50.0% | |
| CAAAGGCAACAAGAGCGGAC+CGG | - | chr5.4:57347988-57348007 | MS.gene26160:CDS | 55.0% | |
| CAAGAGCGGACCGGAAGATA+TGG | - | chr5.4:57347997-57348016 | MS.gene26160:CDS | 55.0% | |
| CGGTCCGCTCTTGTTGCCTT+TGG | + | chr5.4:57347990-57348009 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.4 | gene | 57347973 | 57349131 | 57347973 | ID=MS.gene26160 |
| chr5.4 | mRNA | 57347973 | 57349131 | 57347973 | ID=MS.gene26160.t1;Parent=MS.gene26160 |
| chr5.4 | exon | 57349060 | 57349131 | 57349060 | ID=MS.gene26160.t1.exon1;Parent=MS.gene26160.t1 |
| chr5.4 | CDS | 57349060 | 57349131 | 57349060 | ID=cds.MS.gene26160.t1;Parent=MS.gene26160.t1 |
| chr5.4 | exon | 57348941 | 57348977 | 57348941 | ID=MS.gene26160.t1.exon2;Parent=MS.gene26160.t1 |
| chr5.4 | CDS | 57348941 | 57348977 | 57348941 | ID=cds.MS.gene26160.t1;Parent=MS.gene26160.t1 |
| chr5.4 | exon | 57348747 | 57348820 | 57348747 | ID=MS.gene26160.t1.exon3;Parent=MS.gene26160.t1 |
| chr5.4 | CDS | 57348747 | 57348820 | 57348747 | ID=cds.MS.gene26160.t1;Parent=MS.gene26160.t1 |
| chr5.4 | exon | 57348577 | 57348670 | 57348577 | ID=MS.gene26160.t1.exon4;Parent=MS.gene26160.t1 |
| chr5.4 | CDS | 57348577 | 57348670 | 57348577 | ID=cds.MS.gene26160.t1;Parent=MS.gene26160.t1 |
| chr5.4 | exon | 57348401 | 57348483 | 57348401 | ID=MS.gene26160.t1.exon5;Parent=MS.gene26160.t1 |
| chr5.4 | CDS | 57348401 | 57348483 | 57348401 | ID=cds.MS.gene26160.t1;Parent=MS.gene26160.t1 |
| chr5.4 | exon | 57347973 | 57348149 | 57347973 | ID=MS.gene26160.t1.exon6;Parent=MS.gene26160.t1 |
| chr5.4 | CDS | 57347973 | 57348149 | 57347973 | ID=cds.MS.gene26160.t1;Parent=MS.gene26160.t1 |
| Gene Sequence |
| Protein sequence |
