AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene26734

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene26734.t1 MTR_4g124380 97.297 111 3 0 1 111 59 169 6.47e-79 229
MS.gene26734.t1 MTR_4g124390 82.883 111 10 1 1 111 45 146 4.27e-62 186
MS.gene26734.t1 MTR_5g010100 37.037 81 45 1 1 81 61 135 4.62e-15 66.6
MS.gene26734.t1 MTR_7g078150 37.838 74 40 1 1 74 70 137 7.32e-15 67.0
MS.gene26734.t1 MTR_8g098770 39.189 74 35 2 1 74 70 133 2.15e-14 65.5
MS.gene26734.t1 MTR_2g044570 44.872 78 35 2 2 78 63 133 2.78e-13 64.3
MS.gene26734.t1 MTR_3g082510 41.667 72 36 1 3 74 81 146 9.98e-13 60.8
MS.gene26734.t1 MTR_8g009190 44.872 78 35 2 2 78 85 155 1.26e-12 62.8
MS.gene26734.t1 MTR_8g028815 39.189 74 37 2 1 74 74 139 1.79e-12 60.5
MS.gene26734.t1 MTR_5g064990 46.296 54 22 1 21 74 143 189 2.08e-12 62.4
MS.gene26734.t1 MTR_3g099650 50.000 54 20 2 21 74 136 182 3.17e-12 61.6
MS.gene26734.t1 MTR_1g082550 48.148 54 21 1 21 74 127 173 5.19e-12 61.2
MS.gene26734.t1 MTR_2g436100 44.444 72 31 3 2 73 102 164 7.62e-11 57.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene26734.t1 AT3G43430 53.097 113 41 4 2 110 63 167 8.07e-35 117
MS.gene26734.t1 AT5G20885 53.390 118 39 5 2 108 62 174 2.68e-33 114
MS.gene26734.t1 AT3G61460 39.189 74 39 1 1 74 74 141 7.72e-16 69.3
MS.gene26734.t1 AT5G41400 41.558 77 37 3 1 75 84 154 1.59e-15 68.6
MS.gene26734.t1 AT4G00305 41.558 77 38 2 1 77 52 121 6.69e-15 65.9
MS.gene26734.t1 AT1G63840 45.333 75 31 4 1 74 72 137 2.12e-14 65.5
MS.gene26734.t1 AT3G60220 44.872 78 35 2 2 78 97 167 3.33e-13 64.3
MS.gene26734.t1 AT2G01150 53.704 54 18 2 21 74 74 120 6.65e-13 61.2
MS.gene26734.t1 AT2G01150 53.704 54 18 2 21 74 100 146 8.13e-13 61.6
MS.gene26734.t1 AT2G01150 53.704 54 18 2 21 74 123 169 1.28e-12 61.6
MS.gene26734.t1 AT3G05200 39.726 73 36 2 2 73 108 173 5.10e-12 61.2
MS.gene26734.t1 AT2G28920 45.455 55 23 1 19 73 91 138 2.17e-11 57.4
MS.gene26734.t1 AT1G15100 46.269 67 29 2 21 87 86 145 3.54e-11 57.0
MS.gene26734.t1 AT2G04240 36.111 72 39 2 3 74 85 149 8.51e-11 55.8
MS.gene26734.t1 AT2G04240 36.111 72 39 2 3 74 85 149 8.51e-11 55.8

Find 18 sgRNAs with CRISPR-Local

Find 17 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCACATGTGTCTTCTGTTTC+TGG 0.137505 8.3:+4895537 None:intergenic
GAGAGACTTCTTTATCTCTT+TGG 0.276051 8.3:-4895288 MS.gene26734:CDS
ATGCTACCTTTGACAAGTTT+CGG 0.327811 8.3:-4895579 MS.gene26734:CDS
TTCTTCTCCTCAACCTAGTT+GGG 0.344519 8.3:-4895316 MS.gene26734:CDS
TTGTTTGAACAAGCTGAAAA+TGG 0.363273 8.3:-4895511 MS.gene26734:CDS
CTCTGCAAAGTGGACAAGTT+TGG 0.371945 8.3:+4895391 None:intergenic
TTTCTTCTCCTCAACCTAGT+TGG 0.373720 8.3:-4895317 MS.gene26734:CDS
GACAAGTTTCGGAGAAATCA+AGG 0.419612 8.3:-4895568 MS.gene26734:CDS
ATAAGGAATGCATAGATAAG+TGG 0.495925 8.3:-4895446 MS.gene26734:CDS
CTGTGATCATGTGTTTCATA+AGG 0.517073 8.3:-4895463 MS.gene26734:CDS
AGTCTCTCAACAGCCCAACT+AGG 0.548383 8.3:+4895303 None:intergenic
TCAACAGCCCAACTAGGTTG+AGG 0.559804 8.3:+4895309 None:intergenic
TCATCTGAGATTTCAAGCTG+TGG 0.568106 8.3:-4895342 MS.gene26734:CDS
ATCAAAGGAGCTCTGCAAAG+TGG 0.587711 8.3:+4895381 None:intergenic
TAACTAATTATTAATTAACA+AGG 0.588753 8.3:+4895252 None:intergenic
ATTTCTCCGAAACTTGTCAA+AGG 0.593001 8.3:+4895573 None:intergenic
AGTGAATAAGAATTAATCAA+AGG 0.595804 8.3:+4895366 None:intergenic
ATAGATAAGTGGTTAGAACA+TGG 0.680848 8.3:-4895435 MS.gene26734:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AGTGAATAAGAATTAATCAA+AGG + chr8.3:4895482-4895501 None:intergenic 20.0%
ATAAGGAATGCATAGATAAG+TGG - chr8.3:4895399-4895418 MS.gene26734:CDS 30.0%
ATAGATAAGTGGTTAGAACA+TGG - chr8.3:4895410-4895429 MS.gene26734:CDS 30.0%
TTGTTTGAACAAGCTGAAAA+TGG - chr8.3:4895334-4895353 MS.gene26734:CDS 30.0%
ATGCTACCTTTGACAAGTTT+CGG - chr8.3:4895266-4895285 MS.gene26734:CDS 35.0%
ATTTCTCCGAAACTTGTCAA+AGG + chr8.3:4895275-4895294 None:intergenic 35.0%
CTGTGATCATGTGTTTCATA+AGG - chr8.3:4895382-4895401 MS.gene26734:CDS 35.0%
GAGAGACTTCTTTATCTCTT+TGG - chr8.3:4895557-4895576 MS.gene26734:CDS 35.0%
GACAAGTTTCGGAGAAATCA+AGG - chr8.3:4895277-4895296 MS.gene26734:CDS 40.0%
TTCTTCTCCTCAACCTAGTT+GGG - chr8.3:4895529-4895548 MS.gene26734:CDS 40.0%
TTTCTTCTCCTCAACCTAGT+TGG - chr8.3:4895528-4895547 MS.gene26734:CDS 40.0%
! TCATCTGAGATTTCAAGCTG+TGG - chr8.3:4895503-4895522 MS.gene26734:CDS 40.0%
ATCAAAGGAGCTCTGCAAAG+TGG + chr8.3:4895467-4895486 None:intergenic 45.0%
CTCTGCAAAGTGGACAAGTT+TGG + chr8.3:4895457-4895476 None:intergenic 45.0%
GCACATGTGTCTTCTGTTTC+TGG + chr8.3:4895311-4895330 None:intergenic 45.0%
AGTCTCTCAACAGCCCAACT+AGG + chr8.3:4895545-4895564 None:intergenic 50.0%
TCAACAGCCCAACTAGGTTG+AGG + chr8.3:4895539-4895558 None:intergenic 50.0%


Chromosome Type Strat End Strand Name
chr8.3 gene 4895266 4895601 4895266 ID=MS.gene26734
chr8.3 mRNA 4895266 4895601 4895266 ID=MS.gene26734.t1;Parent=MS.gene26734
chr8.3 exon 4895266 4895601 4895266 ID=MS.gene26734.t1.exon1;Parent=MS.gene26734.t1
chr8.3 CDS 4895266 4895601 4895266 ID=cds.MS.gene26734.t1;Parent=MS.gene26734.t1
Gene Sequence

>MS.gene26734

ATGCTACCTTTGACAAGTTTCGGAGAAATCAAGGAAAGACATCCAGAAACAGAAGACACATGTGCTGTTTGTTTGAACAAGCTGAAAATGGAAGATGAAGTTAGAGAATTGATGAACTGTGATCATGTGTTTCATAAGGAATGCATAGATAAGTGGTTAGAACATGGTCACGAAAATGAAAATCATAACCAAACTTGTCCACTTTGCAGAGCTCCTTTGATTAATTCTTATTCACTTTCATCTGAGATTTCAAGCTGTGGATTTTCTTCTCCTCAACCTAGTTGGGCTGTTGAGAGACTTCTTTATCTCTTTGGTGATGATCTCTTGCCTTGTTAA
Protein sequence

>MS.gene26734.t1

MLPLTSFGEIKERHPETEDTCAVCLNKLKMEDEVRELMNCDHVFHKECIDKWLEHGHENENHNQTCPLCRAPLINSYSLSSEISSCGFSSPQPSWAVERLLYLFGDDLLPC