Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57944.t1 | XP_003610228.1 | 95.1 | 184 | 6 | 3 | 1 | 183 | 1 | 182 | 2.20E-90 | 341.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57944.t1 | Q9M0C3 | 43.6 | 133 | 66 | 2 | 21 | 149 | 33 | 160 | 1.8e-21 | 104.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57944.t1 | G7JEB1 | 95.1 | 184 | 6 | 3 | 1 | 183 | 1 | 182 | 1.6e-90 | 341.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049134 | MS.gene57944 | 0.849455 | 3.20E-60 | -1.69E-46 |
| MS.gene049150 | MS.gene57944 | -0.841992 | 3.39E-58 | -1.69E-46 |
| MS.gene049151 | MS.gene57944 | -0.800212 | 1.60E-48 | -1.69E-46 |
| MS.gene049153 | MS.gene57944 | -0.840005 | 1.13E-57 | -1.69E-46 |
| MS.gene049195 | MS.gene57944 | -0.807549 | 4.79E-50 | -1.69E-46 |
| MS.gene049260 | MS.gene57944 | -0.811127 | 8.18E-51 | -1.69E-46 |
| MS.gene049378 | MS.gene57944 | -0.80206 | 6.71E-49 | -1.69E-46 |
| MS.gene049758 | MS.gene57944 | -0.836893 | 7.16E-57 | -1.69E-46 |
| MS.gene049910 | MS.gene57944 | -0.830933 | 2.22E-55 | -1.69E-46 |
| MS.gene049993 | MS.gene57944 | -0.814877 | 1.23E-51 | -1.69E-46 |
| MS.gene050226 | MS.gene57944 | -0.800612 | 1.33E-48 | -1.69E-46 |
| MS.gene050227 | MS.gene57944 | -0.824051 | 9.95E-54 | -1.69E-46 |
| MS.gene050228 | MS.gene57944 | -0.806482 | 8.05E-50 | -1.69E-46 |
| MS.gene050301 | MS.gene57944 | -0.82739 | 1.61E-54 | -1.69E-46 |
| MS.gene050397 | MS.gene57944 | -0.813859 | 2.07E-51 | -1.69E-46 |
| MS.gene050426 | MS.gene57944 | 0.830255 | 3.25E-55 | -1.69E-46 |
| MS.gene050464 | MS.gene57944 | -0.831742 | 1.40E-55 | -1.69E-46 |
| MS.gene050529 | MS.gene57944 | -0.818828 | 1.60E-52 | -1.69E-46 |
| MS.gene050623 | MS.gene57944 | 0.83011 | 3.53E-55 | -1.69E-46 |
| MS.gene050642 | MS.gene57944 | -0.83224 | 1.06E-55 | -1.69E-46 |
| MS.gene050670 | MS.gene57944 | -0.818347 | 2.06E-52 | -1.69E-46 |
| MS.gene050787 | MS.gene57944 | -0.830093 | 3.57E-55 | -1.69E-46 |
| MS.gene050820 | MS.gene57944 | -0.805661 | 1.20E-49 | -1.69E-46 |
| MS.gene050971 | MS.gene57944 | -0.808511 | 2.99E-50 | -1.69E-46 |
| MS.gene05119 | MS.gene57944 | 0.807599 | 4.67E-50 | -1.69E-46 |
| MS.gene051525 | MS.gene57944 | -0.82221 | 2.68E-53 | -1.69E-46 |
| MS.gene051582 | MS.gene57944 | -0.813963 | 1.96E-51 | -1.69E-46 |
| MS.gene051860 | MS.gene57944 | -0.90152 | 2.47E-78 | -1.69E-46 |
| MS.gene051983 | MS.gene57944 | -0.856962 | 2.26E-62 | -1.69E-46 |
| MS.gene052011 | MS.gene57944 | 0.871397 | 7.05E-67 | -1.69E-46 |
| MS.gene052077 | MS.gene57944 | -0.855458 | 6.23E-62 | -1.69E-46 |
| MS.gene052128 | MS.gene57944 | 0.809442 | 1.89E-50 | -1.69E-46 |
| MS.gene052178 | MS.gene57944 | -0.805359 | 1.39E-49 | -1.69E-46 |
| MS.gene052188 | MS.gene57944 | -0.818406 | 1.99E-52 | -1.69E-46 |
| MS.gene052253 | MS.gene57944 | -0.854691 | 1.04E-61 | -1.69E-46 |
| MS.gene052255 | MS.gene57944 | -0.802785 | 4.76E-49 | -1.69E-46 |
| MS.gene05233 | MS.gene57944 | 0.808207 | 3.47E-50 | -1.69E-46 |
| MS.gene052340 | MS.gene57944 | -0.821387 | 4.15E-53 | -1.69E-46 |
| MS.gene052485 | MS.gene57944 | -0.80869 | 2.74E-50 | -1.69E-46 |
| MS.gene052917 | MS.gene57944 | 0.802916 | 4.47E-49 | -1.69E-46 |
| MS.gene053557 | MS.gene57944 | -0.802877 | 4.56E-49 | -1.69E-46 |
| MS.gene053836 | MS.gene57944 | -0.867691 | 1.14E-65 | -1.69E-46 |
| MS.gene054010 | MS.gene57944 | -0.818621 | 1.78E-52 | -1.69E-46 |
| MS.gene054011 | MS.gene57944 | -0.819631 | 1.05E-52 | -1.69E-46 |
| MS.gene054012 | MS.gene57944 | -0.818083 | 2.36E-52 | -1.69E-46 |
| MS.gene054013 | MS.gene57944 | -0.808629 | 2.82E-50 | -1.69E-46 |
| MS.gene054183 | MS.gene57944 | -0.87604 | 1.92E-68 | -1.69E-46 |
| MS.gene054473 | MS.gene57944 | -0.845097 | 5.02E-59 | -1.69E-46 |
| MS.gene054542 | MS.gene57944 | -0.8084 | 3.15E-50 | -1.69E-46 |
| MS.gene054556 | MS.gene57944 | -0.813389 | 2.62E-51 | -1.69E-46 |
| MS.gene054713 | MS.gene57944 | -0.838064 | 3.59E-57 | -1.69E-46 |
| MS.gene054719 | MS.gene57944 | -0.80656 | 7.75E-50 | -1.69E-46 |
| MS.gene054918 | MS.gene57944 | -0.827535 | 1.48E-54 | -1.69E-46 |
| MS.gene054930 | MS.gene57944 | -0.808695 | 2.73E-50 | -1.69E-46 |
| MS.gene054983 | MS.gene57944 | -0.827406 | 1.59E-54 | -1.69E-46 |
| MS.gene055116 | MS.gene57944 | -0.815066 | 1.12E-51 | -1.69E-46 |
| MS.gene055277 | MS.gene57944 | -0.808027 | 3.79E-50 | -1.69E-46 |
| MS.gene055422 | MS.gene57944 | -0.824923 | 6.20E-54 | -1.69E-46 |
| MS.gene055428 | MS.gene57944 | -0.808451 | 3.08E-50 | -1.69E-46 |
| MS.gene05553 | MS.gene57944 | -0.836088 | 1.15E-56 | -1.69E-46 |
| MS.gene055601 | MS.gene57944 | 0.801206 | 1.00E-48 | -1.69E-46 |
| MS.gene055604 | MS.gene57944 | -0.817964 | 2.51E-52 | -1.69E-46 |
| MS.gene055859 | MS.gene57944 | -0.809001 | 2.35E-50 | -1.69E-46 |
| MS.gene056191 | MS.gene57944 | -0.802955 | 4.39E-49 | -1.69E-46 |
| MS.gene056447 | MS.gene57944 | -0.832354 | 9.92E-56 | -1.69E-46 |
| MS.gene056723 | MS.gene57944 | -0.827866 | 1.23E-54 | -1.69E-46 |
| MS.gene056899 | MS.gene57944 | 0.852332 | 4.95E-61 | -1.69E-46 |
| MS.gene056979 | MS.gene57944 | -0.859756 | 3.32E-63 | -1.69E-46 |
| MS.gene05702 | MS.gene57944 | -0.806722 | 7.17E-50 | -1.69E-46 |
| MS.gene057121 | MS.gene57944 | -0.812631 | 3.85E-51 | -1.69E-46 |
| MS.gene057245 | MS.gene57944 | -0.812929 | 3.31E-51 | -1.69E-46 |
| MS.gene057448 | MS.gene57944 | -0.82063 | 6.20E-53 | -1.69E-46 |
| MS.gene057449 | MS.gene57944 | -0.81154 | 6.65E-51 | -1.69E-46 |
| MS.gene057450 | MS.gene57944 | -0.813279 | 2.77E-51 | -1.69E-46 |
| MS.gene057469 | MS.gene57944 | -0.855749 | 5.12E-62 | -1.69E-46 |
| MS.gene057476 | MS.gene57944 | -0.80067 | 1.29E-48 | -1.69E-46 |
| MS.gene057749 | MS.gene57944 | -0.804831 | 1.79E-49 | -1.69E-46 |
| MS.gene057823 | MS.gene57944 | -0.800722 | 1.26E-48 | -1.69E-46 |
| MS.gene058002 | MS.gene57944 | -0.831612 | 1.51E-55 | -1.69E-46 |
| MS.gene058042 | MS.gene57944 | -0.810696 | 1.01E-50 | -1.69E-46 |
| MS.gene05811 | MS.gene57944 | -0.855106 | 7.88E-62 | -1.69E-46 |
| MS.gene058139 | MS.gene57944 | -0.809549 | 1.79E-50 | -1.69E-46 |
| MS.gene058142 | MS.gene57944 | -0.821236 | 4.49E-53 | -1.69E-46 |
| MS.gene058218 | MS.gene57944 | 0.82009 | 8.24E-53 | -1.69E-46 |
| MS.gene058710 | MS.gene57944 | -0.847697 | 9.82E-60 | -1.69E-46 |
| MS.gene05886 | MS.gene57944 | -0.831642 | 1.49E-55 | -1.69E-46 |
| MS.gene058928 | MS.gene57944 | -0.801077 | 1.07E-48 | -1.69E-46 |
| MS.gene05907 | MS.gene57944 | -0.853556 | 2.21E-61 | -1.69E-46 |
| MS.gene059247 | MS.gene57944 | -0.800294 | 1.54E-48 | -1.69E-46 |
| MS.gene059285 | MS.gene57944 | -0.823028 | 1.73E-53 | -1.69E-46 |
| MS.gene059294 | MS.gene57944 | -0.844341 | 8.03E-59 | -1.69E-46 |
| MS.gene059420 | MS.gene57944 | -0.858615 | 7.29E-63 | -1.69E-46 |
| MS.gene059462 | MS.gene57944 | -0.809236 | 2.09E-50 | -1.69E-46 |
| MS.gene059597 | MS.gene57944 | -0.807686 | 4.48E-50 | -1.69E-46 |
| MS.gene059609 | MS.gene57944 | -0.836342 | 9.90E-57 | -1.69E-46 |
| MS.gene059688 | MS.gene57944 | -0.812161 | 4.87E-51 | -1.69E-46 |
| MS.gene05984 | MS.gene57944 | -0.88221 | 1.26E-70 | -1.69E-46 |
| MS.gene059894 | MS.gene57944 | -0.80429 | 2.32E-49 | -1.69E-46 |
| MS.gene060403 | MS.gene57944 | -0.814859 | 1.24E-51 | -1.69E-46 |
| MS.gene060802 | MS.gene57944 | -0.801313 | 9.55E-49 | -1.69E-46 |
| MS.gene060986 | MS.gene57944 | -0.810888 | 9.22E-51 | -1.69E-46 |
| MS.gene061049 | MS.gene57944 | -0.846986 | 1.54E-59 | -1.69E-46 |
| MS.gene061114 | MS.gene57944 | 0.858485 | 7.98E-63 | -1.69E-46 |
| MS.gene061154 | MS.gene57944 | -0.808277 | 3.35E-50 | -1.69E-46 |
| MS.gene061159 | MS.gene57944 | -0.820049 | 8.42E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene68410 | MS.gene57944 | PPI |
| MS.gene57944 | MS.gene26734 | PPI |
| MS.gene57944 | MS.gene030212 | PPI |
| MS.gene57944 | MS.gene26733 | PPI |
| MS.gene57944 | MS.gene68411 | PPI |
| MS.gene68413 | MS.gene57944 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57944.t1 | MTR_4g129335 | 95.652 | 184 | 5 | 3 | 1 | 183 | 1 | 182 | 1.35e-98 | 283 |
| MS.gene57944.t1 | MTR_4g129370 | 95.652 | 184 | 5 | 3 | 1 | 183 | 1 | 182 | 1.35e-98 | 283 |
| MS.gene57944.t1 | MTR_3g078120 | 33.099 | 142 | 82 | 4 | 29 | 159 | 1 | 140 | 7.73e-17 | 73.9 |
| MS.gene57944.t1 | MTR_6g007680 | 32.432 | 148 | 87 | 6 | 31 | 177 | 1 | 136 | 3.56e-14 | 67.0 |
| MS.gene57944.t1 | MTR_3g114500 | 36.207 | 116 | 67 | 4 | 33 | 147 | 5 | 114 | 3.56e-13 | 63.5 |
| MS.gene57944.t1 | MTR_2g041200 | 31.148 | 122 | 73 | 3 | 39 | 149 | 33 | 154 | 6.72e-13 | 63.9 |
| MS.gene57944.t1 | MTR_8g009190 | 34.307 | 137 | 72 | 4 | 26 | 149 | 19 | 150 | 7.19e-13 | 65.9 |
| MS.gene57944.t1 | MTR_4g063070 | 32.331 | 133 | 65 | 6 | 37 | 146 | 248 | 378 | 1.21e-12 | 65.5 |
| MS.gene57944.t1 | MTR_7g076250 | 34.667 | 75 | 48 | 1 | 81 | 154 | 66 | 140 | 1.84e-12 | 63.2 |
| MS.gene57944.t1 | MTR_2g055790 | 35.772 | 123 | 68 | 4 | 36 | 153 | 33 | 149 | 4.98e-12 | 62.0 |
| MS.gene57944.t1 | MTR_6g007670 | 38.462 | 91 | 49 | 2 | 64 | 153 | 137 | 221 | 5.07e-12 | 62.8 |
| MS.gene57944.t1 | MTR_5g064980 | 30.469 | 128 | 72 | 3 | 31 | 146 | 57 | 179 | 5.64e-12 | 63.5 |
| MS.gene57944.t1 | MTR_3g078470 | 40.845 | 71 | 40 | 1 | 84 | 152 | 82 | 152 | 8.68e-12 | 61.6 |
| MS.gene57944.t1 | MTR_4g106680 | 29.936 | 157 | 93 | 5 | 26 | 165 | 40 | 196 | 1.16e-11 | 62.4 |
| MS.gene57944.t1 | MTR_4g108830 | 42.424 | 66 | 37 | 1 | 84 | 149 | 155 | 219 | 1.43e-11 | 61.2 |
| MS.gene57944.t1 | MTR_5g064990 | 28.369 | 141 | 88 | 3 | 31 | 163 | 67 | 202 | 1.51e-11 | 62.4 |
| MS.gene57944.t1 | MTR_1g026070 | 33.043 | 115 | 70 | 3 | 33 | 146 | 5 | 113 | 1.65e-11 | 59.3 |
| MS.gene57944.t1 | MTR_7g116200 | 34.568 | 81 | 52 | 1 | 84 | 164 | 74 | 153 | 1.85e-11 | 60.1 |
| MS.gene57944.t1 | MTR_8g066740 | 31.206 | 141 | 84 | 5 | 29 | 159 | 22 | 159 | 1.91e-11 | 61.6 |
| MS.gene57944.t1 | MTR_4g108830 | 42.424 | 66 | 37 | 1 | 84 | 149 | 127 | 191 | 1.94e-11 | 60.5 |
| MS.gene57944.t1 | MTR_7g073200 | 35.065 | 77 | 50 | 0 | 82 | 158 | 85 | 161 | 2.10e-11 | 60.1 |
| MS.gene57944.t1 | MTR_8g073120 | 30.579 | 121 | 78 | 3 | 34 | 148 | 22 | 142 | 2.49e-11 | 60.5 |
| MS.gene57944.t1 | MTR_3g110190 | 30.769 | 130 | 85 | 3 | 27 | 152 | 12 | 140 | 2.74e-11 | 61.2 |
| MS.gene57944.t1 | MTR_5g076990 | 31.613 | 155 | 95 | 5 | 3 | 151 | 4 | 153 | 2.84e-11 | 61.2 |
| MS.gene57944.t1 | MTR_1g492830 | 26.241 | 141 | 100 | 1 | 21 | 157 | 1 | 141 | 3.39e-11 | 60.1 |
| MS.gene57944.t1 | MTR_4g120270 | 41.772 | 79 | 42 | 2 | 84 | 162 | 86 | 160 | 4.01e-11 | 60.5 |
| MS.gene57944.t1 | MTR_7g096690 | 29.932 | 147 | 83 | 5 | 22 | 154 | 10 | 150 | 4.25e-11 | 60.8 |
| MS.gene57944.t1 | MTR_8g066780 | 31.288 | 163 | 75 | 8 | 11 | 153 | 47 | 192 | 5.48e-11 | 60.5 |
| MS.gene57944.t1 | MTR_4g030070 | 34.940 | 83 | 54 | 0 | 74 | 156 | 80 | 162 | 5.55e-11 | 59.7 |
| MS.gene57944.t1 | MTR_2g436100 | 39.394 | 99 | 57 | 2 | 84 | 179 | 99 | 197 | 5.77e-11 | 60.1 |
| MS.gene57944.t1 | MTR_7g116220 | 38.806 | 67 | 38 | 1 | 84 | 147 | 76 | 142 | 6.51e-11 | 58.9 |
| MS.gene57944.t1 | MTR_2g090905 | 36.585 | 82 | 46 | 1 | 81 | 156 | 42 | 123 | 6.81e-11 | 57.4 |
| MS.gene57944.t1 | MTR_2g035800 | 40.000 | 80 | 48 | 0 | 82 | 161 | 80 | 159 | 7.72e-11 | 58.2 |
| MS.gene57944.t1 | MTR_5g092750 | 46.939 | 49 | 26 | 0 | 101 | 149 | 182 | 230 | 7.74e-11 | 59.3 |
| MS.gene57944.t1 | MTR_5g022920 | 30.702 | 114 | 58 | 2 | 54 | 146 | 118 | 231 | 7.90e-11 | 59.3 |
| MS.gene57944.t1 | MTR_8g089880 | 36.250 | 80 | 51 | 0 | 84 | 163 | 133 | 212 | 8.07e-11 | 59.7 |
| MS.gene57944.t1 | MTR_3g464780 | 40.278 | 72 | 42 | 1 | 99 | 170 | 127 | 197 | 8.15e-11 | 59.7 |
| MS.gene57944.t1 | MTR_3g099650 | 32.283 | 127 | 67 | 5 | 30 | 146 | 61 | 178 | 9.30e-11 | 60.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57944.t1 | AT2G18670 | 40.252 | 159 | 82 | 3 | 16 | 173 | 24 | 170 | 2.01e-32 | 115 |
| MS.gene57944.t1 | AT4G30370 | 39.231 | 130 | 78 | 1 | 20 | 149 | 32 | 160 | 1.07e-25 | 97.4 |
| MS.gene57944.t1 | AT2G17450 | 30.872 | 149 | 84 | 6 | 15 | 152 | 13 | 153 | 2.12e-14 | 68.2 |
| MS.gene57944.t1 | AT2G42360 | 33.333 | 150 | 93 | 4 | 17 | 160 | 20 | 168 | 2.45e-14 | 68.9 |
| MS.gene57944.t1 | AT4G09100 | 31.707 | 123 | 65 | 4 | 39 | 149 | 15 | 130 | 6.16e-14 | 65.9 |
| MS.gene57944.t1 | AT3G10910 | 26.437 | 174 | 110 | 3 | 4 | 165 | 7 | 174 | 1.14e-13 | 66.2 |
| MS.gene57944.t1 | AT3G20395 | 41.096 | 73 | 41 | 1 | 84 | 156 | 153 | 223 | 5.51e-13 | 65.1 |
| MS.gene57944.t1 | AT2G27940 | 35.227 | 88 | 49 | 2 | 84 | 164 | 121 | 207 | 1.28e-12 | 64.3 |
| MS.gene57944.t1 | AT3G62690 | 41.333 | 75 | 43 | 1 | 81 | 155 | 90 | 163 | 2.11e-12 | 63.9 |
| MS.gene57944.t1 | AT4G10150 | 27.152 | 151 | 98 | 2 | 8 | 146 | 3 | 153 | 2.54e-12 | 63.5 |
| MS.gene57944.t1 | AT5G42200 | 30.303 | 132 | 77 | 3 | 29 | 152 | 28 | 152 | 2.86e-12 | 62.0 |
| MS.gene57944.t1 | AT3G61460 | 31.973 | 147 | 82 | 4 | 42 | 178 | 23 | 161 | 2.89e-12 | 62.4 |
| MS.gene57944.t1 | AT5G01880 | 34.091 | 88 | 56 | 1 | 61 | 146 | 60 | 147 | 5.26e-12 | 61.2 |
| MS.gene57944.t1 | AT5G05280 | 44.828 | 58 | 32 | 0 | 102 | 159 | 111 | 168 | 8.13e-12 | 61.2 |
| MS.gene57944.t1 | AT5G27420 | 28.049 | 164 | 104 | 4 | 9 | 164 | 26 | 183 | 8.96e-12 | 62.8 |
| MS.gene57944.t1 | AT4G10160 | 35.443 | 79 | 51 | 0 | 68 | 146 | 61 | 139 | 2.13e-11 | 60.8 |
| MS.gene57944.t1 | AT3G05200 | 28.485 | 165 | 100 | 5 | 6 | 162 | 30 | 184 | 2.66e-11 | 61.6 |
| MS.gene57944.t1 | AT1G63840 | 34.884 | 129 | 67 | 6 | 32 | 147 | 10 | 134 | 4.66e-11 | 58.9 |
| MS.gene57944.t1 | AT5G66070 | 33.036 | 112 | 54 | 2 | 54 | 146 | 109 | 218 | 7.29e-11 | 59.3 |
| MS.gene57944.t1 | AT5G66070 | 33.036 | 112 | 54 | 2 | 54 | 146 | 109 | 218 | 7.29e-11 | 59.3 |
| MS.gene57944.t1 | AT2G17730 | 31.818 | 132 | 65 | 3 | 43 | 149 | 110 | 241 | 7.62e-11 | 59.7 |
Find 79 sgRNAs with CRISPR-Local
Find 81 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATGAAGGTTCTTCACGATTT+TGG | 0.131959 | 8.4:+5329520 | MS.gene57944:CDS |
| GGAAAAGGGAGAGGAGATTA+GGG | 0.247255 | 8.4:-5329083 | None:intergenic |
| AGGAAAAGGGAGAGGAGATT+AGG | 0.279449 | 8.4:-5329084 | None:intergenic |
| AGGAGATTAGGGTTTGGTTT+TGG | 0.298382 | 8.4:-5329072 | None:intergenic |
| TTCAGGATCGCCATGGTGTT+GGG | 0.309331 | 8.4:-5329043 | None:intergenic |
| TTCTCGTTGTGCTGTTTGTT+TGG | 0.334977 | 8.4:+5329332 | MS.gene57944:CDS |
| TATTGATACCTGGCTTCTTA+AGG | 0.367630 | 8.4:+5329422 | MS.gene57944:CDS |
| TCCCTCTTCTTTCTCTTCCT+CGG | 0.367925 | 8.4:+5329135 | MS.gene57944:CDS |
| CGCTCAACCTTCACAAGGTT+CGG | 0.370509 | 8.4:+5329485 | MS.gene57944:CDS |
| CCTTTGGTGATCCGGAAGTT+GGG | 0.379531 | 8.4:-5329291 | None:intergenic |
| TGATGATGGGTTTGAGGAAA+AGG | 0.391533 | 8.4:-5329098 | None:intergenic |
| TCACAAGCTATTATTACAAA+AGG | 0.402643 | 8.4:-5329572 | None:intergenic |
| TCAACAACCTCCCCAACTTC+CGG | 0.405396 | 8.4:+5329280 | MS.gene57944:CDS |
| ACAAACAGCACAACGAGAAT+CGG | 0.409644 | 8.4:-5329328 | None:intergenic |
| AGGGAGAGGAGATTAGGGTT+TGG | 0.413011 | 8.4:-5329078 | None:intergenic |
| GACGGAAACGGAGGCGGTGG+AGG | 0.416739 | 8.4:-5329203 | None:intergenic |
| ACCTTTGGTGATCCGGAAGT+TGG | 0.426029 | 8.4:-5329292 | None:intergenic |
| AAACGGAACCTTTGGTGATC+CGG | 0.428137 | 8.4:-5329299 | None:intergenic |
| TAGGACGGAAACGGAGGCGG+TGG | 0.438475 | 8.4:-5329206 | None:intergenic |
| CGTTGTGCTGTTTGTTTGGA+CGG | 0.442179 | 8.4:+5329336 | MS.gene57944:CDS |
| GAAGAGGTTGAGGAGGAGAA+AGG | 0.454979 | 8.4:-5329166 | None:intergenic |
| GGGTTCAGAAACGGAACCTT+TGG | 0.469591 | 8.4:-5329307 | None:intergenic |
| ACGGAGGCGGTGGAGGGCGC+CGG | 0.472988 | 8.4:-5329196 | None:intergenic |
| CGGCTAAGAGGAAGAGGTTG+AGG | 0.481229 | 8.4:-5329176 | None:intergenic |
| ACGAGAATCGGGTTCAGAAA+CGG | 0.482086 | 8.4:-5329316 | None:intergenic |
| GTTCAGGATCGCCATGGTGT+TGG | 0.482441 | 8.4:-5329044 | None:intergenic |
| GAAGCATGATGATGGGTTTG+AGG | 0.492199 | 8.4:-5329104 | None:intergenic |
| ACCTTGTGAAGGTTGAGCGT+TGG | 0.493031 | 8.4:-5329481 | None:intergenic |
| TGTGAGGAGAAGCATGATGA+TGG | 0.495135 | 8.4:-5329112 | None:intergenic |
| CAAACAGCACAACGAGAATC+GGG | 0.497987 | 8.4:-5329327 | None:intergenic |
| GGTGGAGGGCGCCGGCTAAG+AGG | 0.499890 | 8.4:-5329188 | None:intergenic |
| GGTTGAGCGTTGGACCGAAC+CGG | 0.501540 | 8.4:-5329471 | None:intergenic |
| CTCAACCTCTTCCTCTTAGC+CGG | 0.507892 | 8.4:+5329177 | MS.gene57944:CDS |
| GATGATGGGTTTGAGGAAAA+GGG | 0.516706 | 8.4:-5329097 | None:intergenic |
| AGAGAAAGAAGAGGGATGTG+AGG | 0.517778 | 8.4:-5329128 | None:intergenic |
| CACAAGCTATTATTACAAAA+GGG | 0.519132 | 8.4:-5329571 | None:intergenic |
| ATCGGAGATGTATTGATACC+TGG | 0.519657 | 8.4:+5329412 | MS.gene57944:CDS |
| ACGGAAACGGAGGCGGTGGA+GGG | 0.523526 | 8.4:-5329202 | None:intergenic |
| TGGGTTTGAGGAAAAGGGAG+AGG | 0.528031 | 8.4:-5329092 | None:intergenic |
| CTTTGGTGATCCGGAAGTTG+GGG | 0.528986 | 8.4:-5329290 | None:intergenic |
| CTACTTGCCGTACACCGGTT+CGG | 0.530310 | 8.4:+5329457 | MS.gene57944:CDS |
| GAGTAGGACGGAAACGGAGG+CGG | 0.531377 | 8.4:-5329209 | None:intergenic |
| GGGCGCCGGCTAAGAGGAAG+AGG | 0.535078 | 8.4:-5329182 | None:intergenic |
| GGAGAAAGGCGGCGAAACCG+AGG | 0.539177 | 8.4:-5329152 | None:intergenic |
| GGTGAGGGATTGAAGACGGC+GGG | 0.544512 | 8.4:-5329235 | None:intergenic |
| TGAAGACGGCGGGTTGGAGT+AGG | 0.545882 | 8.4:-5329225 | None:intergenic |
| CGTTGGACCGAACCGGTGTA+CGG | 0.546340 | 8.4:-5329464 | None:intergenic |
| AGGGATTGAAGACGGCGGGT+TGG | 0.557895 | 8.4:-5329231 | None:intergenic |
| GGGAGGTTGTTGATTTCGCG+AGG | 0.564332 | 8.4:-5329270 | None:intergenic |
| AGGGAGGAAAGCGTCGGTGA+GGG | 0.565102 | 8.4:-5329250 | None:intergenic |
| CCATGGTGTTGGGGATGAGA+AGG | 0.567157 | 8.4:-5329033 | None:intergenic |
| GGGTTGGAGTAGGACGGAAA+CGG | 0.569430 | 8.4:-5329215 | None:intergenic |
| TCGGTTGTAGAACAAGATGA+AGG | 0.570752 | 8.4:+5329504 | MS.gene57944:CDS |
| CGAACCGGTGTACGGCAAGT+AGG | 0.574174 | 8.4:-5329456 | None:intergenic |
| CCCAACTTCCGGATCACCAA+AGG | 0.581918 | 8.4:+5329291 | MS.gene57944:CDS |
| CGTGGCAACCTTAAGAAGCC+AGG | 0.587413 | 8.4:-5329430 | None:intergenic |
| TGGTGATCCGGAAGTTGGGG+AGG | 0.590502 | 8.4:-5329287 | None:intergenic |
| GGTTGTTGATTTCGCGAGGG+AGG | 0.591963 | 8.4:-5329266 | None:intergenic |
| CTAAGAGGAAGAGGTTGAGG+AGG | 0.593800 | 8.4:-5329173 | None:intergenic |
| GTGTCCTACTTGCCGTACAC+CGG | 0.602666 | 8.4:+5329452 | MS.gene57944:CDS |
| GTTGTTGTTCAGGATCGCCA+TGG | 0.605538 | 8.4:-5329050 | None:intergenic |
| GTGAGGAGAAGCATGATGAT+GGG | 0.607789 | 8.4:-5329111 | None:intergenic |
| ACCGAGGAAGAGAAAGAAGA+GGG | 0.608693 | 8.4:-5329136 | None:intergenic |
| AACCGAGGAAGAGAAAGAAG+AGG | 0.612058 | 8.4:-5329137 | None:intergenic |
| TCAGGATCGCCATGGTGTTG+GGG | 0.615546 | 8.4:-5329042 | None:intergenic |
| CGTCGGTGAGGGATTGAAGA+CGG | 0.626086 | 8.4:-5329239 | None:intergenic |
| GTGTTGGGGATGAGAAGGCA+TGG | 0.640252 | 8.4:-5329028 | None:intergenic |
| GAGGGAGGAAAGCGTCGGTG+AGG | 0.640961 | 8.4:-5329251 | None:intergenic |
| TCTACAACCGAACCTTGTGA+AGG | 0.648198 | 8.4:-5329492 | None:intergenic |
| TTGGAGTAGGACGGAAACGG+AGG | 0.653534 | 8.4:-5329212 | None:intergenic |
| GAGGTTGAGGAGGAGAAAGG+CGG | 0.653553 | 8.4:-5329163 | None:intergenic |
| CCTTCTCATCCCCAACACCA+TGG | 0.654904 | 8.4:+5329033 | MS.gene57944:CDS |
| GGAGGTTGTTGATTTCGCGA+GGG | 0.655842 | 8.4:-5329269 | None:intergenic |
| CGGTGAGGGATTGAAGACGG+CGG | 0.657153 | 8.4:-5329236 | None:intergenic |
| TTCGCGAGGGAGGAAAGCGT+CGG | 0.671242 | 8.4:-5329256 | None:intergenic |
| TGTAGAAATCTCGCTGCCTG+TGG | 0.675543 | 8.4:+5329378 | MS.gene57944:CDS |
| GACGGCGGGTTGGAGTAGGA+CGG | 0.677108 | 8.4:-5329221 | None:intergenic |
| TCCAACGCTCAACCTTCACA+AGG | 0.693145 | 8.4:+5329480 | MS.gene57944:CDS |
| TGTACGGCAAGTAGGACACG+TGG | 0.730422 | 8.4:-5329448 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| TATTGATACCTGGCTTCTTA+AGG | + | chr8.4:5329422-5329441 | MS.gene57944:CDS | 35.0% | |
| ! | ATGAAGGTTCTTCACGATTT+TGG | + | chr8.4:5329520-5329539 | MS.gene57944:CDS | 35.0% |
| !!! | TTTGGTTTTGGTTGTTGTTC+AGG | - | chr8.4:5329063-5329082 | None:intergenic | 35.0% |
| ACAAACAGCACAACGAGAAT+CGG | - | chr8.4:5329331-5329350 | None:intergenic | 40.0% | |
| TCGGTTGTAGAACAAGATGA+AGG | + | chr8.4:5329504-5329523 | MS.gene57944:CDS | 40.0% | |
| ! | ACGGTTTTCGTAATGGTCAA+TGG | + | chr8.4:5329355-5329374 | MS.gene57944:CDS | 40.0% |
| ! | ATCGGAGATGTATTGATACC+TGG | + | chr8.4:5329412-5329431 | MS.gene57944:CDS | 40.0% |
| ! | CCTGTGGTCATGTTTTTCAT+CGG | + | chr8.4:5329394-5329413 | MS.gene57944:CDS | 40.0% |
| ! | TGTTTGGACGGTTTTCGTAA+TGG | + | chr8.4:5329348-5329367 | MS.gene57944:CDS | 40.0% |
| !! | AGGAGATTAGGGTTTGGTTT+TGG | - | chr8.4:5329075-5329094 | None:intergenic | 40.0% |
| !! | GATGATGGGTTTGAGGAAAA+GGG | - | chr8.4:5329100-5329119 | None:intergenic | 40.0% |
| !! | TGATGATGGGTTTGAGGAAA+AGG | - | chr8.4:5329101-5329120 | None:intergenic | 40.0% |
| !! | TTCTCGTTGTGCTGTTTGTT+TGG | + | chr8.4:5329332-5329351 | MS.gene57944:CDS | 40.0% |
| AACCGAGGAAGAGAAAGAAG+AGG | - | chr8.4:5329140-5329159 | None:intergenic | 45.0% | |
| ACCGAGGAAGAGAAAGAAGA+GGG | - | chr8.4:5329139-5329158 | None:intergenic | 45.0% | |
| ACGAGAATCGGGTTCAGAAA+CGG | - | chr8.4:5329319-5329338 | None:intergenic | 45.0% | |
| AGAGAAAGAAGAGGGATGTG+AGG | - | chr8.4:5329131-5329150 | None:intergenic | 45.0% | |
| AGGAAAAGGGAGAGGAGATT+AGG | - | chr8.4:5329087-5329106 | None:intergenic | 45.0% | |
| CAAACAGCACAACGAGAATC+GGG | - | chr8.4:5329330-5329349 | None:intergenic | 45.0% | |
| CCGATGAAAAACATGACCAC+AGG | - | chr8.4:5329397-5329416 | None:intergenic | 45.0% | |
| GGAAAAGGGAGAGGAGATTA+GGG | - | chr8.4:5329086-5329105 | None:intergenic | 45.0% | |
| TCCCTCTTCTTTCTCTTCCT+CGG | + | chr8.4:5329135-5329154 | MS.gene57944:CDS | 45.0% | |
| TCTACAACCGAACCTTGTGA+AGG | - | chr8.4:5329495-5329514 | None:intergenic | 45.0% | |
| ! | AAACGGAACCTTTGGTGATC+CGG | - | chr8.4:5329302-5329321 | None:intergenic | 45.0% |
| ! | GTGAGGAGAAGCATGATGAT+GGG | - | chr8.4:5329114-5329133 | None:intergenic | 45.0% |
| ! | TGTGAGGAGAAGCATGATGA+TGG | - | chr8.4:5329115-5329134 | None:intergenic | 45.0% |
| !! | CGTTGTGCTGTTTGTTTGGA+CGG | + | chr8.4:5329336-5329355 | MS.gene57944:CDS | 45.0% |
| !! | GAAGCATGATGATGGGTTTG+AGG | - | chr8.4:5329107-5329126 | None:intergenic | 45.0% |
| ACCTTGTGAAGGTTGAGCGT+TGG | - | chr8.4:5329484-5329503 | None:intergenic | 50.0% | |
| AGGGAGAGGAGATTAGGGTT+TGG | - | chr8.4:5329081-5329100 | None:intergenic | 50.0% | |
| CGCTCAACCTTCACAAGGTT+CGG | + | chr8.4:5329485-5329504 | MS.gene57944:CDS | 50.0% | |
| CTAAGAGGAAGAGGTTGAGG+AGG | - | chr8.4:5329176-5329195 | None:intergenic | 50.0% | |
| CTCAACCTCTTCCTCTTAGC+CGG | + | chr8.4:5329177-5329196 | MS.gene57944:CDS | 50.0% | |
| GAAGAGGTTGAGGAGGAGAA+AGG | - | chr8.4:5329169-5329188 | None:intergenic | 50.0% | |
| GGGTTCAGAAACGGAACCTT+TGG | - | chr8.4:5329310-5329329 | None:intergenic | 50.0% | |
| TCAACAACCTCCCCAACTTC+CGG | + | chr8.4:5329280-5329299 | MS.gene57944:CDS | 50.0% | |
| TCCAACGCTCAACCTTCACA+AGG | + | chr8.4:5329480-5329499 | MS.gene57944:CDS | 50.0% | |
| TGGGTTTGAGGAAAAGGGAG+AGG | - | chr8.4:5329095-5329114 | None:intergenic | 50.0% | |
| TGTAGAAATCTCGCTGCCTG+TGG | + | chr8.4:5329378-5329397 | MS.gene57944:CDS | 50.0% | |
| TTCAGGATCGCCATGGTGTT+GGG | - | chr8.4:5329046-5329065 | None:intergenic | 50.0% | |
| ! | ACCTTTGGTGATCCGGAAGT+TGG | - | chr8.4:5329295-5329314 | None:intergenic | 50.0% |
| ! | CCTTTGGTGATCCGGAAGTT+GGG | - | chr8.4:5329294-5329313 | None:intergenic | 50.0% |
| ! | CTTTGGTGATCCGGAAGTTG+GGG | - | chr8.4:5329293-5329312 | None:intergenic | 50.0% |
| ! | GTTGTTGTTCAGGATCGCCA+TGG | - | chr8.4:5329053-5329072 | None:intergenic | 50.0% |
| !! | GGAGGTTGTTGATTTCGCGA+GGG | - | chr8.4:5329272-5329291 | None:intergenic | 50.0% |
| CCCAACTTCCGGATCACCAA+AGG | + | chr8.4:5329291-5329310 | MS.gene57944:CDS | 55.0% | |
| CCTTCTCATCCCCAACACCA+TGG | + | chr8.4:5329033-5329052 | MS.gene57944:CDS | 55.0% | |
| CGGCTAAGAGGAAGAGGTTG+AGG | - | chr8.4:5329179-5329198 | None:intergenic | 55.0% | |
| CGTCGGTGAGGGATTGAAGA+CGG | - | chr8.4:5329242-5329261 | None:intergenic | 55.0% | |
| CGTGGCAACCTTAAGAAGCC+AGG | - | chr8.4:5329433-5329452 | None:intergenic | 55.0% | |
| GAGGTTGAGGAGGAGAAAGG+CGG | - | chr8.4:5329166-5329185 | None:intergenic | 55.0% | |
| GGGTTGGAGTAGGACGGAAA+CGG | - | chr8.4:5329218-5329237 | None:intergenic | 55.0% | |
| GTGTCCTACTTGCCGTACAC+CGG | + | chr8.4:5329452-5329471 | MS.gene57944:CDS | 55.0% | |
| GTTCAGGATCGCCATGGTGT+TGG | - | chr8.4:5329047-5329066 | None:intergenic | 55.0% | |
| TGTACGGCAAGTAGGACACG+TGG | - | chr8.4:5329451-5329470 | None:intergenic | 55.0% | |
| TTGGAGTAGGACGGAAACGG+AGG | - | chr8.4:5329215-5329234 | None:intergenic | 55.0% | |
| ! | CCATGGTGTTGGGGATGAGA+AGG | - | chr8.4:5329036-5329055 | None:intergenic | 55.0% |
| ! | CTACTTGCCGTACACCGGTT+CGG | + | chr8.4:5329457-5329476 | MS.gene57944:CDS | 55.0% |
| ! | TCAGGATCGCCATGGTGTTG+GGG | - | chr8.4:5329045-5329064 | None:intergenic | 55.0% |
| !! | GGGAGGTTGTTGATTTCGCG+AGG | - | chr8.4:5329273-5329292 | None:intergenic | 55.0% |
| !! | GGTTGTTGATTTCGCGAGGG+AGG | - | chr8.4:5329269-5329288 | None:intergenic | 55.0% |
| AGGGATTGAAGACGGCGGGT+TGG | - | chr8.4:5329234-5329253 | None:intergenic | 60.0% | |
| CGAACCGGTGTACGGCAAGT+AGG | - | chr8.4:5329459-5329478 | None:intergenic | 60.0% | |
| CGGTGAGGGATTGAAGACGG+CGG | - | chr8.4:5329239-5329258 | None:intergenic | 60.0% | |
| CGTTGGACCGAACCGGTGTA+CGG | - | chr8.4:5329467-5329486 | None:intergenic | 60.0% | |
| GAGTAGGACGGAAACGGAGG+CGG | - | chr8.4:5329212-5329231 | None:intergenic | 60.0% | |
| GGTGAGGGATTGAAGACGGC+GGG | - | chr8.4:5329238-5329257 | None:intergenic | 60.0% | |
| TGGTGATCCGGAAGTTGGGG+AGG | - | chr8.4:5329290-5329309 | None:intergenic | 60.0% | |
| ! | TTCGCGAGGGAGGAAAGCGT+CGG | - | chr8.4:5329259-5329278 | None:intergenic | 60.0% |
| !! | AGGGAGGAAAGCGTCGGTGA+GGG | - | chr8.4:5329253-5329272 | None:intergenic | 60.0% |
| !! | GGTTGAGCGTTGGACCGAAC+CGG | - | chr8.4:5329474-5329493 | None:intergenic | 60.0% |
| !! | TGAAGACGGCGGGTTGGAGT+AGG | - | chr8.4:5329228-5329247 | None:intergenic | 60.0% |
| ACGGAAACGGAGGCGGTGGA+GGG | - | chr8.4:5329205-5329224 | None:intergenic | 65.0% | |
| GGAGAAAGGCGGCGAAACCG+AGG | - | chr8.4:5329155-5329174 | None:intergenic | 65.0% | |
| TAGGACGGAAACGGAGGCGG+TGG | - | chr8.4:5329209-5329228 | None:intergenic | 65.0% | |
| !! | GACGGCGGGTTGGAGTAGGA+CGG | - | chr8.4:5329224-5329243 | None:intergenic | 65.0% |
| !! | GAGGGAGGAAAGCGTCGGTG+AGG | - | chr8.4:5329254-5329273 | None:intergenic | 65.0% |
| GACGGAAACGGAGGCGGTGG+AGG | - | chr8.4:5329206-5329225 | None:intergenic | 70.0% | |
| !! | GGGCGCCGGCTAAGAGGAAG+AGG | - | chr8.4:5329185-5329204 | None:intergenic | 70.0% |
| !! | GGTGGAGGGCGCCGGCTAAG+AGG | - | chr8.4:5329191-5329210 | None:intergenic | 75.0% |
| !! | ACGGAGGCGGTGGAGGGCGC+CGG | - | chr8.4:5329199-5329218 | None:intergenic | 80.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.4 | gene | 5329030 | 5329581 | 5329030 | ID=MS.gene57944 |
| chr8.4 | mRNA | 5329030 | 5329581 | 5329030 | ID=MS.gene57944.t1;Parent=MS.gene57944 |
| chr8.4 | exon | 5329030 | 5329581 | 5329030 | ID=MS.gene57944.t1.exon1;Parent=MS.gene57944.t1 |
| chr8.4 | CDS | 5329030 | 5329581 | 5329030 | ID=cds.MS.gene57944.t1;Parent=MS.gene57944.t1 |
| Gene Sequence |
| Protein sequence |
