AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene26741


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene26741.t1 MTR_4g123563 96.721 122 4 0 1 122 206 327 7.06e-82 249
MS.gene26741.t1 MTR_2g045420 71.311 122 35 0 1 122 213 334 2.10e-62 199
MS.gene26741.t1 MTR_2g045420 58.784 148 35 1 1 122 213 360 7.74e-57 184
MS.gene26741.t1 MTR_2g045420 58.389 149 35 1 1 122 213 361 8.85e-57 184
MS.gene26741.t1 MTR_7g111530 60.000 120 48 0 3 122 216 335 1.10e-52 172
MS.gene26741.t1 MTR_7g111530 60.000 120 48 0 3 122 216 335 2.93e-52 172
MS.gene26741.t1 MTR_7g111530 60.000 120 48 0 3 122 223 342 3.28e-52 172
MS.gene26741.t1 MTR_1g484940 58.197 122 51 0 1 122 218 339 2.74e-50 167
MS.gene26741.t1 MTR_4g132420 55.932 118 52 0 1 118 229 346 2.29e-45 154
MS.gene26741.t1 MTR_2g023910 54.386 114 52 0 5 118 223 336 8.74e-43 147
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene26741.t1 AT1G11910 55.085 118 53 0 1 118 215 332 5.39e-47 158
MS.gene26741.t1 AT1G11910 55.085 118 53 0 1 118 226 343 7.06e-47 158
MS.gene26741.t1 AT1G62290 55.932 118 52 0 1 118 222 339 1.35e-46 155
MS.gene26741.t1 AT1G62290 55.932 118 52 0 1 118 222 339 3.55e-46 155
MS.gene26741.t1 AT1G62290 55.932 118 52 0 1 118 222 339 3.55e-46 155
MS.gene26741.t1 AT1G62290 55.932 118 52 0 1 118 222 339 5.19e-46 155
MS.gene26741.t1 AT1G62290 55.932 118 52 0 1 118 222 339 8.26e-46 155
MS.gene26741.t1 AT1G62290 55.932 118 52 0 1 118 222 339 8.26e-46 155
MS.gene26741.t1 AT4G04460 52.800 125 58 1 1 124 220 344 1.33e-44 152
MS.gene26741.t1 AT4G04460 55.263 114 51 0 1 114 220 333 8.31e-44 150
MS.gene26741.t1 AT4G22050 39.796 98 56 2 1 96 178 274 2.16e-14 68.6
MS.gene26741.t1 AT1G69100 35.922 103 57 5 1 95 187 288 3.39e-12 62.4
MS.gene26741.t1 AT1G69100 35.922 103 57 5 1 95 179 280 4.15e-12 62.4

Find 32 sgRNAs with CRISPR-Local

Find 95 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAATGTTTGAATCTGGGTTT+TGG 0.227356 8.3:+4943639 None:intergenic
TCACCTCCAATGTTTGAATC+TGG 0.287305 8.3:+4943632 None:intergenic
ATACCTATGGTGATTCGATA+TGG 0.307900 8.3:-4942162 MS.gene26741:CDS
ATATGGGAATTCATAACTAA+TGG 0.363388 8.3:-4942145 MS.gene26741:CDS
CACCTCCAATGTTTGAATCT+GGG 0.367366 8.3:+4943633 None:intergenic
TATGGGAATTCATAACTAAT+GGG 0.370287 8.3:-4942144 MS.gene26741:CDS
CAATAGCCCTGTTGAGAAAC+TGG 0.374977 8.3:+4943554 None:intergenic
TTATGTTCCAGTTTCTCAAC+AGG 0.378641 8.3:-4943561 MS.gene26741:CDS
TCAGGAACATCTTTAATTGC+TGG 0.405484 8.3:-4942361 MS.gene26741:CDS
CTACTTGAAAACAATTCAAC+AGG 0.412952 8.3:-4943417 MS.gene26741:intron
GAGAAACTGGAACATAAGTA+TGG 0.414422 8.3:+4943567 None:intergenic
CAGTTTCTCAACAGGGCTAT+TGG 0.422671 8.3:-4943553 MS.gene26741:CDS
TTTCATCTTTAGATTGATGT+TGG 0.476903 8.3:-4943447 MS.gene26741:intron
GGAGGTGAGATTGTGTTTGG+TGG 0.478829 8.3:-4943617 MS.gene26741:CDS
ATTGGAGGTGAGATTGTGTT+TGG 0.484906 8.3:-4943620 MS.gene26741:CDS
TACCTATGGTGATTCGATAT+GGG 0.484972 8.3:-4942161 MS.gene26741:CDS
TTGTGTTTGGTGGTATTGAC+TGG 0.485792 8.3:-4943607 MS.gene26741:CDS
TTCTCAACAGGGCTATTGGC+AGG 0.489968 8.3:-4943549 MS.gene26741:intron
CAAAACCCAGATTCAAACAT+TGG 0.517182 8.3:-4943638 MS.gene26741:CDS
AACCATGCCATTGGAGCTGA+AGG 0.517861 8.3:-4942217 MS.gene26741:CDS
ATATCGAATCACCATAGGTA+TGG 0.523540 8.3:+4942164 None:intergenic
AACATATCCTTCAGCTCCAA+TGG 0.541608 8.3:+4942210 None:intergenic
ACTCAAATTAACCATGCCAT+TGG 0.549157 8.3:-4942226 MS.gene26741:CDS
ATCCTTCAGCTCCAATGGCA+TGG 0.576643 8.3:+4942215 None:intergenic
AACCCAGATTCAAACATTGG+AGG 0.625934 8.3:-4943635 MS.gene26741:CDS
TATGTTCCAGTTTCTCAACA+GGG 0.628853 8.3:-4943560 MS.gene26741:CDS
ATGGGAATTCATAACTAATG+GGG 0.628958 8.3:-4942143 MS.gene26741:CDS
TGTGCCACGATTATTGACTC+AGG 0.635375 8.3:-4942379 MS.gene26741:CDS
TTCCCATATCGAATCACCAT+AGG 0.643813 8.3:+4942159 None:intergenic
AAATCAATAGGGCTATGTCA+AGG 0.677582 8.3:-4942406 MS.gene26741:intron
TGTTCCTGAGTCAATAATCG+TGG 0.680517 8.3:+4942375 None:intergenic
TCAATAGGGCTATGTCAAGG+TGG 0.702696 8.3:-4942403 MS.gene26741:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AACTAAGTTTTTTTTTTTTA+AGG + chr8.3:4943036-4943055 None:intergenic 10.0%
!!! ATAACTATATTCATATTTTA+TGG - chr8.3:4942264-4942283 MS.gene26741:intron 10.0%
!!! CTTTTTTTGTTATTTTTTTA+TGG - chr8.3:4943469-4943488 MS.gene26741:intron 10.0%
!!! TAACTATATTCATATTTTAT+GGG - chr8.3:4942265-4942284 MS.gene26741:intron 10.0%
!!! TTTTTTTTTTTTGATAATTG+TGG + chr8.3:4942761-4942780 None:intergenic 10.0%
!! TATAATGTTATTATCTTTAG+CGG + chr8.3:4943110-4943129 None:intergenic 15.0%
!!! AAAACTTAACTTATCTTTTT+TGG + chr8.3:4942960-4942979 None:intergenic 15.0%
!!! AAATATATGTTTTGAAAAAG+TGG - chr8.3:4943134-4943153 MS.gene26741:intron 15.0%
! AAAAATATCATCCATACCTA+TGG - chr8.3:4943601-4943620 MS.gene26741:CDS 25.0%
! AGGGATTTATATATGAAACA+TGG + chr8.3:4942409-4942428 None:intergenic 25.0%
! ATATAAGCTGTTTATCCAAA+CGG + chr8.3:4942546-4942565 None:intergenic 25.0%
! ATATGGGAATTCATAACTAA+TGG - chr8.3:4943631-4943650 MS.gene26741:CDS 25.0%
! CCACTGTAAAAAAAAATTAG+TGG - chr8.3:4942440-4942459 MS.gene26741:intron 25.0%
! CTCTTACGATATAAAAGTTA+AGG - chr8.3:4942518-4942537 MS.gene26741:intron 25.0%
! GGCTTATTAAAATATGACAT+AGG - chr8.3:4943231-4943250 MS.gene26741:intron 25.0%
! TATATTGATGCTTATAGCAT+TGG - chr8.3:4942561-4942580 MS.gene26741:intron 25.0%
! TATGGGAATTCATAACTAAT+GGG - chr8.3:4943632-4943651 MS.gene26741:CDS 25.0%
! TTTCATCTTTAGATTGATGT+TGG - chr8.3:4942329-4942348 MS.gene26741:intron 25.0%
!! CTTACATAAATTGAAGTGTT+TGG - chr8.3:4942664-4942683 MS.gene26741:intron 25.0%
!!! CCACTAATTTTTTTTTACAG+TGG + chr8.3:4942443-4942462 None:intergenic 25.0%
!!! TATGGTACACTTTTTTCATT+AGG - chr8.3:4943487-4943506 MS.gene26741:intron 25.0%
ACCAAAAATCAATGATGACA+TGG + chr8.3:4942307-4942326 None:intergenic 30.0%
ATGGGAATTCATAACTAATG+GGG - chr8.3:4943633-4943652 MS.gene26741:CDS 30.0%
ATGTTAAGATGTCATACTGT+TGG + chr8.3:4943442-4943461 None:intergenic 30.0%
CTACTTGAAAACAATTCAAC+AGG - chr8.3:4942359-4942378 MS.gene26741:CDS 30.0%
CTCAATAAGCTATTTACGTT+TGG - chr8.3:4942622-4942641 MS.gene26741:intron 30.0%
TATAAGCTGTTTATCCAAAC+GGG + chr8.3:4942545-4942564 None:intergenic 30.0%
TCAAAATAAGCCAATCCAAA+CGG - chr8.3:4943342-4943361 MS.gene26741:intron 30.0%
! CTCAAAAGATTTTCTCACTT+TGG - chr8.3:4942109-4942128 MS.gene26741:CDS 30.0%
!! ATTTTGAGTGTGAGGATAAA+AGG + chr8.3:4943672-4943691 None:intergenic 30.0%
!! TATTGACTGGAGACATTTTA+GGG - chr8.3:4942182-4942201 MS.gene26741:CDS 30.0%
!! TTTTGAGTGTGAGGATAAAA+GGG + chr8.3:4943671-4943690 None:intergenic 30.0%
!!! ACCATGTCATCATTGATTTT+TGG - chr8.3:4942303-4942322 MS.gene26741:intron 30.0%
AAAATAAGCCAATCCAAACG+GGG - chr8.3:4943344-4943363 MS.gene26741:intron 35.0%
AAATCAATAGGGCTATGTCA+AGG - chr8.3:4943370-4943389 MS.gene26741:intron 35.0%
AACACCTTCAAAAAGGCTTA+TGG - chr8.3:4943297-4943316 MS.gene26741:intron 35.0%
ACACCTTCAAAAAGGCTTAT+GGG - chr8.3:4943298-4943317 MS.gene26741:intron 35.0%
ACTCAAATTAACCATGCCAT+TGG - chr8.3:4943550-4943569 MS.gene26741:CDS 35.0%
ATAAGCTGTTTATCCAAACG+GGG + chr8.3:4942544-4942563 None:intergenic 35.0%
ATACCTATGGTGATTCGATA+TGG - chr8.3:4943614-4943633 MS.gene26741:CDS 35.0%
ATATCGAATCACCATAGGTA+TGG + chr8.3:4943615-4943634 None:intergenic 35.0%
CAAAACCCAGATTCAAACAT+TGG - chr8.3:4942138-4942157 MS.gene26741:CDS 35.0%
CAAAATAAGCCAATCCAAAC+GGG - chr8.3:4943343-4943362 MS.gene26741:intron 35.0%
GAGAAACTGGAACATAAGTA+TGG + chr8.3:4942212-4942231 None:intergenic 35.0%
TACCTATGGTGATTCGATAT+GGG - chr8.3:4943615-4943634 MS.gene26741:CDS 35.0%
TATGTTCCAGTTTCTCAACA+GGG - chr8.3:4942216-4942235 MS.gene26741:CDS 35.0%
TCAGGAACATCTTTAATTGC+TGG - chr8.3:4943415-4943434 MS.gene26741:intron 35.0%
TTACCCAAACACCTTCAAAA+AGG - chr8.3:4943290-4943309 MS.gene26741:intron 35.0%
TTATGTTCCAGTTTCTCAAC+AGG - chr8.3:4942215-4942234 MS.gene26741:CDS 35.0%
! ATTGACTGGAGACATTTTAG+GGG - chr8.3:4942183-4942202 MS.gene26741:CDS 35.0%
! CAATGTTTGAATCTGGGTTT+TGG + chr8.3:4942140-4942159 None:intergenic 35.0%
! TAAATTTTCAGCTTACCCTC+CGG + chr8.3:4943211-4943230 None:intergenic 35.0%
!! TGACATAGCCCTATTGATTT+AGG + chr8.3:4943370-4943389 None:intergenic 35.0%
!!! AAGCCTTTTTGAAGGTGTTT+GGG + chr8.3:4943296-4943315 None:intergenic 35.0%
!!! GGCTTATTTTGAGCTTATGT+GGG + chr8.3:4943334-4943353 None:intergenic 35.0%
!!! GTATTGACTGGAGACATTTT+AGG - chr8.3:4942181-4942200 MS.gene26741:CDS 35.0%
!!! TAAGCCTTTTTGAAGGTGTT+TGG + chr8.3:4943297-4943316 None:intergenic 35.0%
!!! TGGCTTATTTTGAGCTTATG+TGG + chr8.3:4943335-4943354 None:intergenic 35.0%
AAACGGGGCCTAAATCAATA+GGG - chr8.3:4943359-4943378 MS.gene26741:intron 40.0%
AACATATCCTTCAGCTCCAA+TGG + chr8.3:4943569-4943588 None:intergenic 40.0%
AACCCAGATTCAAACATTGG+AGG - chr8.3:4942141-4942160 MS.gene26741:CDS 40.0%
ATAAAAGTTAAGGCCCCGTT+TGG - chr8.3:4942528-4942547 MS.gene26741:intron 40.0%
ATTGGAGGTGAGATTGTGTT+TGG - chr8.3:4942156-4942175 MS.gene26741:CDS 40.0%
CACCTCCAATGTTTGAATCT+GGG + chr8.3:4942146-4942165 None:intergenic 40.0%
GGAGGGTAAGCTGAAAATTT+AGG - chr8.3:4943210-4943229 MS.gene26741:intron 40.0%
TACAGTGGAGTACAGTACAA+GGG + chr8.3:4942428-4942447 None:intergenic 40.0%
TCACCTCCAATGTTTGAATC+TGG + chr8.3:4942147-4942166 None:intergenic 40.0%
TCATAAGCTCCTTGTTAAGC+CGG - chr8.3:4943189-4943208 MS.gene26741:intron 40.0%
TGTTCCTGAGTCAATAATCG+TGG + chr8.3:4943404-4943423 None:intergenic 40.0%
TTACAGTGGAGTACAGTACA+AGG + chr8.3:4942429-4942448 None:intergenic 40.0%
TTCCCATATCGAATCACCAT+AGG + chr8.3:4943620-4943639 None:intergenic 40.0%
! ACTCCCATAAGCCTTTTTGA+AGG + chr8.3:4943304-4943323 None:intergenic 40.0%
! GCTTATAGCATTGGAGCTTA+TGG - chr8.3:4942570-4942589 MS.gene26741:intron 40.0%
! TAGTTCTTGTTTGCAGAGTG+TGG - chr8.3:4943525-4943544 MS.gene26741:intron 40.0%
!! TTGTGTTTGGTGGTATTGAC+TGG - chr8.3:4942169-4942188 MS.gene26741:CDS 40.0%
CAAACGGGGCCTAAATCAAT+AGG - chr8.3:4943358-4943377 MS.gene26741:intron 45.0%
CAATAGCCCTGTTGAGAAAC+TGG + chr8.3:4942225-4942244 None:intergenic 45.0%
CAGTTTCTCAACAGGGCTAT+TGG - chr8.3:4942223-4942242 MS.gene26741:CDS 45.0%
TAACCTCCTTCGATTCTCTC+TGG + chr8.3:4943162-4943181 None:intergenic 45.0%
TCAATAGGGCTATGTCAAGG+TGG - chr8.3:4943373-4943392 MS.gene26741:intron 45.0%
!! CTATTGATTTAGGCCCCGTT+TGG + chr8.3:4943360-4943379 None:intergenic 45.0%
!! TGTGCCACGATTATTGACTC+AGG - chr8.3:4943397-4943416 MS.gene26741:intron 45.0%
!!! AATATATTAATTTTTTTTGA+GGG - chr8.3:4942818-4942837 MS.gene26741:intron 5.0%
!!! TAATATATTAATTTTTTTTG+AGG - chr8.3:4942817-4942836 MS.gene26741:intron 5.0%
!!! TAATATCTATATTTTTTTTA+AGG + chr8.3:4942922-4942941 None:intergenic 5.0%
AACCATGCCATTGGAGCTGA+AGG - chr8.3:4943559-4943578 MS.gene26741:CDS 50.0%
AAGCTCCTTGTTAAGCCGGA+GGG - chr8.3:4943193-4943212 MS.gene26741:intron 50.0%
ATCCTTCAGCTCCAATGGCA+TGG + chr8.3:4943564-4943583 None:intergenic 50.0%
GATTTAGGCCCCGTTTGGAT+TGG + chr8.3:4943355-4943374 None:intergenic 50.0%
GGAGGTGAGATTGTGTTTGG+TGG - chr8.3:4942159-4942178 MS.gene26741:CDS 50.0%
GTGGTTCCAGAGAGAATCGA+AGG - chr8.3:4943153-4943172 MS.gene26741:intron 50.0%
GTTCCAGAGAGAATCGAAGG+AGG - chr8.3:4943156-4943175 MS.gene26741:intron 50.0%
TAAGCTCCTTGTTAAGCCGG+AGG - chr8.3:4943192-4943211 MS.gene26741:intron 50.0%
TTCTCAACAGGGCTATTGGC+AGG - chr8.3:4942227-4942246 MS.gene26741:CDS 50.0%
GCTTACCCTCCGGCTTAACA+AGG + chr8.3:4943201-4943220 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr8.3 gene 4942105 4943693 4942105 ID=MS.gene26741
chr8.3 mRNA 4942105 4943693 4942105 ID=MS.gene26741.t1;Parent=MS.gene26741
chr8.3 exon 4943550 4943693 4943550 ID=MS.gene26741.t1.exon1;Parent=MS.gene26741.t1
chr8.3 CDS 4943550 4943693 4943550 ID=cds.MS.gene26741.t1;Parent=MS.gene26741.t1
chr8.3 exon 4943418 4943457 4943418 ID=MS.gene26741.t1.exon2;Parent=MS.gene26741.t1
chr8.3 CDS 4943418 4943457 4943418 ID=cds.MS.gene26741.t1;Parent=MS.gene26741.t1
chr8.3 exon 4942354 4942418 4942354 ID=MS.gene26741.t1.exon3;Parent=MS.gene26741.t1
chr8.3 CDS 4942354 4942418 4942354 ID=cds.MS.gene26741.t1;Parent=MS.gene26741.t1
chr8.3 exon 4942105 4942257 4942105 ID=MS.gene26741.t1.exon4;Parent=MS.gene26741.t1
chr8.3 CDS 4942105 4942257 4942105 ID=cds.MS.gene26741.t1;Parent=MS.gene26741.t1
Gene Sequence

>MS.gene26741

ATGACTCAAAAGATTTTCTCACTTTGGCTAAACCAAAACCCAGATTCAAACATTGGAGGTGAGATTGTGTTTGGTGGTATTGACTGGAGACATTTTAGGGGTGACCATACTTATGTTCCAGTTTCTCAACAGGGCTATTGGCAGGTATATATTATATTTATAACTATATTCATATTTTATGGGTGTCTCTTATCATCAACCATGTCATCATTGATTTTTGGTGTTTTCATCTTTAGATTGATGTTGGAGACATTCTACTTGAAAACAATTCAACAGGTACTCTTGCTTTTTACATTTCACTCCATGTTTCATATATAAATCCCTTGTACTGTACTCCACTGTAAAAAAAAATTAGTGGTTCAGTTTACATTATTAGTGCATACAAATTACATCATTTTATACTATTAGTTTTTCTCTTACGATATAAAAGTTAAGGCCCCGTTTGGATAAACAGCTTATATTGATGCTTATAGCATTGGAGCTTATGGCACTAGAGCTTACATCATAAGCTCTTATACTCAATAAGCTATTTACGTTTGGATATGTACACTAAAAAGTGCTTACATAAATTGAAGTGTTTGGATGTGACATTACATAAGCAATTATTGAAATTTTAAATATTTTTAATTTAACAAAAAAATCAAAGAATCGAACCACAATTATCAAAAAAAAAAAATTGATAAAATTAATCAAGTTGTAAAAAACAATATTATAATATATTAATTTTTTTTGAGGGAATAATATAATTAATATAATATATATATATATATATATATATATATATATATATATATATAGTATAATCAATATTCGTCCTTAAAAAAAATATAGATATTAAAATACAGTTTGTTACCAAAAAAGATAAGTTAAGTTTTTTTTTTTATTCAGTTTCATTTTGTTACGTAACAAAAAAAATAATAAAAATCTTCCTTAAAAAAAAAAAACTTAGTTACTTGTGTTACTTTAGTAAGATACATATATATCAATTTTGTTACTCATGCACCGCTAAAGATAATAACATTATAATTAAAATATATGTTTTGAAAAAGTGGTTCCAGAGAGAATCGAAGGAGGTTACATAAATTCATAAGCTCCTTGTTAAGCCGGAGGGTAAGCTGAAAATTTAGGCTTATTAAAATATGACATAGGCAGCTTATGAAACTACGATAAGCTATTTTTATTTATTTACCCAAACACCTTCAAAAAGGCTTATGGGAGTAGATAAACCCACATAAGCTCAAAATAAGCCAATCCAAACGGGGCCTAAATCAATAGGGCTATGTCAAGGTGGTTGTGCCACGATTATTGACTCAGGAACATCTTTAATTGCTGGTCCAACAGTATGACATCTTAACATTAGACTTCTTTTTTTGTTATTTTTTTATGGTACACTTTTTTCATTAGGTGTATTTTTCTTACTTAGTTCTTGTTTGCAGAGTGTGGTGACTCAAATTAACCATGCCATTGGAGCTGAAGGATATGTTAGTTTTGAATGTAAAAATATCATCCATACCTATGGTGATTCGATATGGGAATTCATAACTAATGGGGTTTGTTCTCTAGCCCTTTTATCCTCACACTCAAAATGA

Protein sequence

>MS.gene26741.t1

MTQKIFSLWLNQNPDSNIGGEIVFGGIDWRHFRGDHTYVPVSQQGYWQIDVGDILLENNSTGLCQGGCATIIDSGTSLIAGPTSVVTQINHAIGAEGYVSFECKNIIHTYGDSIWEFITNGVCSLALLSSHSK