Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene27110.t1 | RHN77685.1 | 93.6 | 140 | 8 | 1 | 1 | 140 | 392 | 530 | 1.60E-64 | 255.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene27110.t1 | Q9SGQ6 | 52.8 | 127 | 59 | 1 | 1 | 126 | 646 | 772 | 1.7e-30 | 133.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene27110.t1 | A0A396JN30 | 93.6 | 140 | 8 | 1 | 1 | 140 | 392 | 530 | 1.1e-64 | 255.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene051490 | MS.gene27110 | 0.803274 | 3.77E-49 | -1.69E-46 |
| MS.gene051492 | MS.gene27110 | 0.818696 | 1.71E-52 | -1.69E-46 |
| MS.gene051744 | MS.gene27110 | 0.804123 | 2.51E-49 | -1.69E-46 |
| MS.gene052677 | MS.gene27110 | 0.803376 | 3.59E-49 | -1.69E-46 |
| MS.gene053296 | MS.gene27110 | 0.80692 | 6.51E-50 | -1.69E-46 |
| MS.gene05410 | MS.gene27110 | 0.806307 | 8.77E-50 | -1.69E-46 |
| MS.gene05423 | MS.gene27110 | 0.806173 | 9.35E-50 | -1.69E-46 |
| MS.gene056699 | MS.gene27110 | 0.804099 | 2.54E-49 | -1.69E-46 |
| MS.gene058081 | MS.gene27110 | 0.832065 | 1.17E-55 | -1.69E-46 |
| MS.gene059439 | MS.gene27110 | 0.812159 | 4.88E-51 | -1.69E-46 |
| MS.gene060373 | MS.gene27110 | 0.816472 | 5.43E-52 | -1.69E-46 |
| MS.gene060520 | MS.gene27110 | 0.802351 | 5.85E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene27110.t1 | MTR_6g069480 | 85.401 | 137 | 19 | 1 | 1 | 137 | 472 | 607 | 8.99e-78 | 241 |
| MS.gene27110.t1 | MTR_6g069480 | 85.401 | 137 | 19 | 1 | 1 | 137 | 666 | 801 | 2.96e-76 | 241 |
| MS.gene27110.t1 | MTR_6g069480 | 85.401 | 137 | 19 | 1 | 1 | 137 | 689 | 824 | 3.13e-76 | 241 |
| MS.gene27110.t1 | MTR_6g069480 | 85.401 | 137 | 19 | 1 | 1 | 137 | 698 | 833 | 3.15e-76 | 242 |
| MS.gene27110.t1 | MTR_1g026480 | 93.578 | 109 | 6 | 1 | 1 | 109 | 103 | 210 | 4.41e-67 | 204 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene27110.t1 | AT1G76280 | 52.756 | 127 | 59 | 1 | 1 | 126 | 674 | 800 | 7.82e-38 | 136 |
| MS.gene27110.t1 | AT1G76280 | 52.756 | 127 | 59 | 1 | 1 | 126 | 678 | 804 | 8.48e-38 | 136 |
| MS.gene27110.t1 | AT1G76280 | 52.756 | 127 | 59 | 1 | 1 | 126 | 646 | 772 | 8.77e-38 | 135 |
Find 24 sgRNAs with CRISPR-Local
Find 28 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCACAATTGCTCTCCTATTA+TGG | 0.249764 | 1.1:+133781 | None:intergenic |
| AACCTGACGTTAGCCTCTTT+TGG | 0.259450 | 1.1:+133804 | None:intergenic |
| TCTGAAGATAAAGTTGCAAT+AGG | 0.325114 | 1.1:-133920 | MS.gene27110:CDS |
| CTAACGTCAGGTTCCATAAT+AGG | 0.331585 | 1.1:-133794 | MS.gene27110:CDS |
| TACAGCAGTAGAAGCATTGC+AGG | 0.375121 | 1.1:-134071 | MS.gene27110:CDS |
| TACTGCTGTATTGTGAAAAC+TGG | 0.375269 | 1.1:+134084 | None:intergenic |
| TGGTCAGCTGACTCACATAT+TGG | 0.396723 | 1.1:+133860 | None:intergenic |
| AGTCAGCTGACCAGAGCCTA+TGG | 0.424315 | 1.1:-133850 | MS.gene27110:CDS |
| AAGACGTAGCCGCATGTTGT+TGG | 0.442831 | 1.1:+134124 | None:intergenic |
| TAGCCGCATGTTGTTGGATC+AGG | 0.451269 | 1.1:+134130 | None:intergenic |
| CTGAAGATAAAGTTGCAATA+GGG | 0.461654 | 1.1:-133919 | MS.gene27110:CDS |
| TAGGGTTGTTGAATTTAAGA+TGG | 0.471021 | 1.1:-133901 | MS.gene27110:CDS |
| TTGCTTGTGATGTCTGTAGA+TGG | 0.471898 | 1.1:-134040 | MS.gene27110:CDS |
| TTAAGATGGTGTGCCATGAT+TGG | 0.474986 | 1.1:-133887 | MS.gene27110:CDS |
| TAAGATGGTGTGCCATGATT+GGG | 0.507685 | 1.1:-133886 | MS.gene27110:CDS |
| GGATGTAATTGAGTTTATTG+TGG | 0.517625 | 1.1:-134179 | MS.gene27110:CDS |
| TCGAGTGGCGATTCCAAAAG+AGG | 0.533718 | 1.1:-133817 | MS.gene27110:CDS |
| ACATATTGGAAACCCAATCA+TGG | 0.558144 | 1.1:+133874 | None:intergenic |
| TTCCAAAAGAGGCTAACGTC+AGG | 0.576549 | 1.1:-133806 | MS.gene27110:CDS |
| GTCAGCTGACCAGAGCCTAT+GGG | 0.596357 | 1.1:-133849 | MS.gene27110:CDS |
| CAGAAGCTAAATCTTCATAG+AGG | 0.601928 | 1.1:+133972 | None:intergenic |
| CAGCCTGATCCAACAACATG+CGG | 0.658980 | 1.1:-134133 | MS.gene27110:CDS |
| GCAGCAACATACTTCAAGAG+AGG | 0.665988 | 1.1:-134018 | MS.gene27110:CDS |
| TATGGGCTAAAAGACTCGAG+TGG | 0.705967 | 1.1:-133832 | MS.gene27110:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| CTGAAGATAAAGTTGCAATA+GGG | - | chr1.1:134043-134062 | MS.gene27110:CDS | 30.0% | |
| GGATGTAATTGAGTTTATTG+TGG | - | chr1.1:133783-133802 | MS.gene27110:CDS | 30.0% | |
| TAGGGTTGTTGAATTTAAGA+TGG | - | chr1.1:134061-134080 | MS.gene27110:CDS | 30.0% | |
| TCTGAAGATAAAGTTGCAAT+AGG | - | chr1.1:134042-134061 | MS.gene27110:CDS | 30.0% | |
| ! | TCAAGAGAGGAAAATTTTTG+TGG | - | chr1.1:133957-133976 | MS.gene27110:CDS | 30.0% |
| ACATATTGGAAACCCAATCA+TGG | + | chr1.1:134091-134110 | None:intergenic | 35.0% | |
| CAGAAGCTAAATCTTCATAG+AGG | + | chr1.1:133993-134012 | None:intergenic | 35.0% | |
| TACTGCTGTATTGTGAAAAC+TGG | + | chr1.1:133881-133900 | None:intergenic | 35.0% | |
| TCACAATTGCTCTCCTATTA+TGG | + | chr1.1:134184-134203 | None:intergenic | 35.0% | |
| CTAACGTCAGGTTCCATAAT+AGG | - | chr1.1:134168-134187 | MS.gene27110:CDS | 40.0% | |
| TAAGATGGTGTGCCATGATT+GGG | - | chr1.1:134076-134095 | MS.gene27110:CDS | 40.0% | |
| TTAAGATGGTGTGCCATGAT+TGG | - | chr1.1:134075-134094 | MS.gene27110:CDS | 40.0% | |
| TTGCTTGTGATGTCTGTAGA+TGG | - | chr1.1:133922-133941 | MS.gene27110:CDS | 40.0% | |
| !! | GATCAGGCTGAACTTTTTCT+TGG | + | chr1.1:133819-133838 | None:intergenic | 40.0% |
| GCAGCAACATACTTCAAGAG+AGG | - | chr1.1:133944-133963 | MS.gene27110:CDS | 45.0% | |
| TTCCAAAAGAGGCTAACGTC+AGG | - | chr1.1:134156-134175 | MS.gene27110:CDS | 45.0% | |
| ! | ACTCGAGTCTTTTAGCCCAT+AGG | + | chr1.1:134131-134150 | None:intergenic | 45.0% |
| ! | TATGGGCTAAAAGACTCGAG+TGG | - | chr1.1:134130-134149 | MS.gene27110:CDS | 45.0% |
| ! | TGGTCAGCTGACTCACATAT+TGG | + | chr1.1:134105-134124 | None:intergenic | 45.0% |
| !! | AACCTGACGTTAGCCTCTTT+TGG | + | chr1.1:134161-134180 | None:intergenic | 45.0% |
| !! | TACAGCAGTAGAAGCATTGC+AGG | - | chr1.1:133891-133910 | MS.gene27110:CDS | 45.0% |
| AAGACGTAGCCGCATGTTGT+TGG | + | chr1.1:133841-133860 | None:intergenic | 50.0% | |
| CAGCCTGATCCAACAACATG+CGG | - | chr1.1:133829-133848 | MS.gene27110:CDS | 50.0% | |
| TAGCCGCATGTTGTTGGATC+AGG | + | chr1.1:133835-133854 | None:intergenic | 50.0% | |
| TCGAGTGGCGATTCCAAAAG+AGG | - | chr1.1:134145-134164 | MS.gene27110:CDS | 50.0% | |
| ! | GTCTTTTAGCCCATAGGCTC+TGG | + | chr1.1:134125-134144 | None:intergenic | 50.0% |
| AGTCAGCTGACCAGAGCCTA+TGG | - | chr1.1:134112-134131 | MS.gene27110:CDS | 55.0% | |
| GTCAGCTGACCAGAGCCTAT+GGG | - | chr1.1:134113-134132 | MS.gene27110:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.1 | gene | 133781 | 134203 | 133781 | ID=MS.gene27110 |
| chr1.1 | mRNA | 133781 | 134203 | 133781 | ID=MS.gene27110.t1;Parent=MS.gene27110 |
| chr1.1 | exon | 133781 | 134203 | 133781 | ID=MS.gene27110.t1.exon1;Parent=MS.gene27110.t1 |
| chr1.1 | CDS | 133781 | 134203 | 133781 | ID=cds.MS.gene27110.t1;Parent=MS.gene27110.t1 |
| Gene Sequence |
| Protein sequence |
