Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene27821.t1 | XP_003629796.1 | 78.1 | 128 | 26 | 1 | 2 | 129 | 58 | 183 | 7.80E-47 | 196.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene27821.t1 | Q9LT27 | 76.9 | 91 | 21 | 0 | 39 | 129 | 95 | 185 | 6.9e-36 | 151.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene27821.t1 | G7LJG7 | 78.1 | 128 | 26 | 1 | 2 | 129 | 58 | 183 | 5.6e-47 | 196.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049351 | MS.gene27821 | 0.820503 | 6.63E-53 | -1.69E-46 |
| MS.gene052188 | MS.gene27821 | 0.804827 | 1.79E-49 | -1.69E-46 |
| MS.gene052310 | MS.gene27821 | 0.814554 | 1.45E-51 | -1.69E-46 |
| MS.gene052485 | MS.gene27821 | 0.800006 | 1.76E-48 | -1.69E-46 |
| MS.gene05811 | MS.gene27821 | 0.804857 | 1.77E-49 | -1.69E-46 |
| MS.gene058293 | MS.gene27821 | 0.819622 | 1.05E-52 | -1.69E-46 |
| MS.gene058660 | MS.gene27821 | 0.820184 | 7.84E-53 | -1.69E-46 |
| MS.gene05907 | MS.gene27821 | 0.802987 | 4.32E-49 | -1.69E-46 |
| MS.gene059597 | MS.gene27821 | 0.802934 | 4.43E-49 | -1.69E-46 |
| MS.gene059676 | MS.gene27821 | 0.806569 | 7.72E-50 | -1.69E-46 |
| MS.gene059960 | MS.gene27821 | 0.804727 | 1.88E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene27821.t1 | MTR_8g086630 | 81.955 | 133 | 22 | 1 | 2 | 134 | 58 | 188 | 1.74e-67 | 208 |
| MS.gene27821.t1 | MTR_4g086490 | 64.662 | 133 | 21 | 2 | 2 | 134 | 58 | 164 | 8.06e-43 | 151 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene27821.t1 | AT3G19810 | 68.966 | 116 | 31 | 2 | 14 | 129 | 75 | 185 | 1.40e-52 | 170 |
| MS.gene27821.t1 | AT3G19810 | 68.966 | 116 | 31 | 2 | 14 | 129 | 75 | 185 | 1.40e-52 | 170 |
Find 28 sgRNAs with CRISPR-Local
Find 41 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCTCAATTTCTTCTTCTTCC+TGG | 0.279746 | 4.1:+13528670 | None:intergenic |
| TCATCACGCTTATGTGTAAT+AGG | 0.323398 | 4.1:-13528625 | MS.gene27821:intron |
| AAGGCACAGTGTCCTTTGAT+TGG | 0.380524 | 4.1:-13528940 | MS.gene27821:CDS |
| TTGCCATAGATTAATCCTTC+AGG | 0.391299 | 4.1:-13528533 | MS.gene27821:intron |
| AAGAAGAAATTGAGGCTTGA+TGG | 0.392126 | 4.1:-13528662 | MS.gene27821:CDS |
| GAAGAAATTGATAAAGATGA+AGG | 0.421786 | 4.1:-13528896 | MS.gene27821:CDS |
| CATGTATGCATCAAAATGTA+TGG | 0.428669 | 4.1:-13528990 | MS.gene27821:CDS |
| AGAAGAAATTGAGGCTTGAT+GGG | 0.452423 | 4.1:-13528661 | MS.gene27821:CDS |
| AGGCACAGTGTCCTTTGATT+GGG | 0.460660 | 4.1:-13528939 | MS.gene27821:CDS |
| ATCTGAACCGGAGTCCCATT+CGG | 0.465444 | 4.1:+13528707 | None:intergenic |
| TGCACTACTTTGGAGAGGTT+AGG | 0.495564 | 4.1:-13528812 | MS.gene27821:CDS |
| ACATCAATAGAAATCTGAAC+CGG | 0.521267 | 4.1:+13528695 | None:intergenic |
| CCCAAATGTTAAAATTTCTG+TGG | 0.541171 | 4.1:-13528509 | MS.gene27821:CDS |
| AAAGGACACTGTGCCTTCCT+CGG | 0.545029 | 4.1:+13528946 | None:intergenic |
| TTCTTCATAGTCCCAATCAA+AGG | 0.549462 | 4.1:+13528928 | None:intergenic |
| CCAGGAAGAAGAAGAAATTG+AGG | 0.557383 | 4.1:-13528670 | MS.gene27821:CDS |
| ACTTTGGAGAGGTTAGGTCT+AGG | 0.558661 | 4.1:-13528806 | MS.gene27821:CDS |
| AGATTTCTATTGATGTTACC+AGG | 0.564204 | 4.1:-13528688 | MS.gene27821:CDS |
| AAGAAATTGATAAAGATGAA+GGG | 0.573818 | 4.1:-13528895 | MS.gene27821:CDS |
| CGTGATTTATACACCGAGGA+AGG | 0.591548 | 4.1:-13528959 | MS.gene27821:CDS |
| GATGAAGGGTCACCGTGGGA+AGG | 0.602100 | 4.1:-13528881 | MS.gene27821:CDS |
| TATATAACCGCACCTTCCCA+CGG | 0.608059 | 4.1:+13528869 | None:intergenic |
| GCGAACTACGAAAGCAGTGA+AGG | 0.615142 | 4.1:-13528735 | MS.gene27821:CDS |
| AGTATTGCACTACTTTGGAG+AGG | 0.618407 | 4.1:-13528817 | MS.gene27821:CDS |
| AGGGTCACCGTGGGAAGGTG+CGG | 0.618932 | 4.1:-13528876 | MS.gene27821:CDS |
| TAAAGATGAAGGGTCACCGT+GGG | 0.665303 | 4.1:-13528885 | MS.gene27821:CDS |
| ATAAAGATGAAGGGTCACCG+TGG | 0.749113 | 4.1:-13528886 | MS.gene27821:CDS |
| TGATCGTGATTTATACACCG+AGG | 0.771445 | 4.1:-13528963 | MS.gene27821:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAGAAATTGATAAAGATGAA+GGG | - | chr4.1:13528526-13528545 | MS.gene27821:CDS | 20.0% |
| ! | AAAAAATAAGGCTTCTGTTA+TGG | - | chr4.1:13528659-13528678 | MS.gene27821:CDS | 25.0% |
| ! | AAAAATAAGGCTTCTGTTAT+GGG | - | chr4.1:13528660-13528679 | MS.gene27821:CDS | 25.0% |
| ! | AACCGATGTTTCAAAAAATA+AGG | - | chr4.1:13528647-13528666 | MS.gene27821:CDS | 25.0% |
| ! | GAAGAAATTGATAAAGATGA+AGG | - | chr4.1:13528525-13528544 | MS.gene27821:CDS | 25.0% |
| !!! | ACCACAGAAATTTTAACATT+TGG | + | chr4.1:13528916-13528935 | None:intergenic | 25.0% |
| !!! | AGCCTTATTTTTTGAAACAT+CGG | + | chr4.1:13528652-13528671 | None:intergenic | 25.0% |
| !!! | CACAGAAATTTTAACATTTG+GGG | + | chr4.1:13528914-13528933 | None:intergenic | 25.0% |
| !!! | CCACAGAAATTTTAACATTT+GGG | + | chr4.1:13528915-13528934 | None:intergenic | 25.0% |
| ACATCAATAGAAATCTGAAC+CGG | + | chr4.1:13528729-13528748 | None:intergenic | 30.0% | |
| AGATTTCTATTGATGTTACC+AGG | - | chr4.1:13528733-13528752 | MS.gene27821:CDS | 30.0% | |
| CCCAAATGTTAAAATTTCTG+TGG | - | chr4.1:13528912-13528931 | MS.gene27821:CDS | 30.0% | |
| !! | TTTTGAGTATTGCACTACTT+TGG | - | chr4.1:13528599-13528618 | MS.gene27821:intron | 30.0% |
| !!! | TTTTTGAAACATCGGTTGAA+AGG | + | chr4.1:13528644-13528663 | None:intergenic | 30.0% |
| AAGAAGAAATTGAGGCTTGA+TGG | - | chr4.1:13528759-13528778 | MS.gene27821:CDS | 35.0% | |
| AGAAGAAATTGAGGCTTGAT+GGG | - | chr4.1:13528760-13528779 | MS.gene27821:CDS | 35.0% | |
| TCATCACGCTTATGTGTAAT+AGG | - | chr4.1:13528796-13528815 | MS.gene27821:CDS | 35.0% | |
| TTCTTCATAGTCCCAATCAA+AGG | + | chr4.1:13528496-13528515 | None:intergenic | 35.0% | |
| TTGCCATAGATTAATCCTTC+AGG | - | chr4.1:13528888-13528907 | MS.gene27821:CDS | 35.0% | |
| CCAGGAAGAAGAAGAAATTG+AGG | - | chr4.1:13528751-13528770 | MS.gene27821:CDS | 40.0% | |
| CCTCAATTTCTTCTTCTTCC+TGG | + | chr4.1:13528754-13528773 | None:intergenic | 40.0% | |
| TGATCGTGATTTATACACCG+AGG | - | chr4.1:13528458-13528477 | MS.gene27821:CDS | 40.0% | |
| ! | AGTATTGCACTACTTTGGAG+AGG | - | chr4.1:13528604-13528623 | MS.gene27821:intron | 40.0% |
| ! | CAGTGAAGGATTTTCCGAAT+GGG | - | chr4.1:13528700-13528719 | MS.gene27821:CDS | 40.0% |
| !! | TTTTAACATTTGGGGCCTGA+AGG | + | chr4.1:13528906-13528925 | None:intergenic | 40.0% |
| AAGGCACAGTGTCCTTTGAT+TGG | - | chr4.1:13528481-13528500 | MS.gene27821:CDS | 45.0% | |
| AGGCACAGTGTCCTTTGATT+GGG | - | chr4.1:13528482-13528501 | MS.gene27821:CDS | 45.0% | |
| ATAAAGATGAAGGGTCACCG+TGG | - | chr4.1:13528535-13528554 | MS.gene27821:intron | 45.0% | |
| CGTGATTTATACACCGAGGA+AGG | - | chr4.1:13528462-13528481 | MS.gene27821:CDS | 45.0% | |
| GGGCCTGAAGGATTAATCTA+TGG | + | chr4.1:13528894-13528913 | None:intergenic | 45.0% | |
| TAAAGATGAAGGGTCACCGT+GGG | - | chr4.1:13528536-13528555 | MS.gene27821:intron | 45.0% | |
| TATATAACCGCACCTTCCCA+CGG | + | chr4.1:13528555-13528574 | None:intergenic | 45.0% | |
| ! | ACTTTGGAGAGGTTAGGTCT+AGG | - | chr4.1:13528615-13528634 | MS.gene27821:intron | 45.0% |
| ! | GCAGTGAAGGATTTTCCGAA+TGG | - | chr4.1:13528699-13528718 | MS.gene27821:CDS | 45.0% |
| ! | TGCACTACTTTGGAGAGGTT+AGG | - | chr4.1:13528609-13528628 | MS.gene27821:intron | 45.0% |
| AAAGGACACTGTGCCTTCCT+CGG | + | chr4.1:13528478-13528497 | None:intergenic | 50.0% | |
| ATCTGAACCGGAGTCCCATT+CGG | + | chr4.1:13528717-13528736 | None:intergenic | 50.0% | |
| GCGAACTACGAAAGCAGTGA+AGG | - | chr4.1:13528686-13528705 | MS.gene27821:CDS | 50.0% | |
| ! | GGATTTTCCGAATGGGACTC+CGG | - | chr4.1:13528707-13528726 | MS.gene27821:CDS | 50.0% |
| GATGAAGGGTCACCGTGGGA+AGG | - | chr4.1:13528540-13528559 | MS.gene27821:intron | 60.0% | |
| AGGGTCACCGTGGGAAGGTG+CGG | - | chr4.1:13528545-13528564 | MS.gene27821:intron | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.1 | gene | 13528432 | 13529011 | 13528432 | ID=MS.gene27821 |
| chr4.1 | mRNA | 13528432 | 13529011 | 13528432 | ID=MS.gene27821.t1;Parent=MS.gene27821 |
| chr4.1 | exon | 13528626 | 13529011 | 13528626 | ID=MS.gene27821.t1.exon1;Parent=MS.gene27821.t1 |
| chr4.1 | CDS | 13528626 | 13529011 | 13528626 | ID=cds.MS.gene27821.t1;Parent=MS.gene27821.t1 |
| chr4.1 | exon | 13528432 | 13528546 | 13528432 | ID=MS.gene27821.t1.exon2;Parent=MS.gene27821.t1 |
| chr4.1 | CDS | 13528432 | 13528546 | 13528432 | ID=cds.MS.gene27821.t1;Parent=MS.gene27821.t1 |
| Gene Sequence |
| Protein sequence |
