Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene27837.t1 | XP_003629835.1 | 96.7 | 120 | 3 | 1 | 1 | 120 | 107 | 225 | 2.40E-61 | 244.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene27837.t1 | P32110 | 38.8 | 103 | 62 | 1 | 15 | 117 | 123 | 224 | 8.3e-15 | 81.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene27837.t1 | G7L788 | 96.7 | 120 | 3 | 1 | 1 | 120 | 107 | 225 | 1.7e-61 | 244.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene055549 | MS.gene27837 | 0.802153 | 6.42E-49 | -1.69E-46 |
| MS.gene056311 | MS.gene27837 | 0.838585 | 2.64E-57 | -1.69E-46 |
| MS.gene058493 | MS.gene27837 | 0.891667 | 3.22E-74 | -1.69E-46 |
| MS.gene058494 | MS.gene27837 | 0.94708 | 1.35E-105 | -1.69E-46 |
| MS.gene058496 | MS.gene27837 | 0.90876 | 1.20E-81 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene27837.t1 | MTR_8g087410 | 96.667 | 120 | 3 | 1 | 1 | 120 | 107 | 225 | 3.14e-80 | 235 |
| MS.gene27837.t1 | MTR_8g087425 | 62.617 | 107 | 39 | 1 | 4 | 110 | 109 | 214 | 2.46e-36 | 124 |
| MS.gene27837.t1 | MTR_6g080440 | 41.905 | 105 | 58 | 2 | 3 | 106 | 106 | 208 | 1.16e-22 | 88.6 |
| MS.gene27837.t1 | MTR_4g019780 | 45.556 | 90 | 48 | 1 | 17 | 106 | 125 | 213 | 6.63e-20 | 81.3 |
| MS.gene27837.t1 | MTR_7g065720 | 42.391 | 92 | 51 | 2 | 15 | 106 | 124 | 213 | 6.60e-18 | 76.3 |
| MS.gene27837.t1 | MTR_1g090150 | 37.037 | 108 | 65 | 2 | 9 | 114 | 111 | 217 | 1.04e-15 | 70.5 |
| MS.gene27837.t1 | MTR_7g065600 | 37.500 | 96 | 58 | 2 | 15 | 110 | 123 | 216 | 4.15e-15 | 68.9 |
| MS.gene27837.t1 | MTR_7g065660 | 34.375 | 96 | 61 | 2 | 15 | 110 | 123 | 216 | 6.60e-15 | 68.2 |
| MS.gene27837.t1 | MTR_7g065290 | 36.458 | 96 | 59 | 2 | 15 | 110 | 123 | 216 | 1.20e-14 | 67.4 |
| MS.gene27837.t1 | MTR_4g019790 | 33.962 | 106 | 68 | 2 | 14 | 119 | 122 | 225 | 1.30e-14 | 67.4 |
| MS.gene27837.t1 | MTR_7g065630 | 30.769 | 117 | 74 | 4 | 4 | 119 | 129 | 239 | 3.34e-14 | 66.6 |
| MS.gene27837.t1 | MTR_7g065590 | 35.417 | 96 | 60 | 2 | 15 | 110 | 123 | 216 | 7.35e-14 | 65.5 |
| MS.gene27837.t1 | MTR_7g065740 | 30.357 | 112 | 74 | 3 | 1 | 110 | 108 | 217 | 1.68e-13 | 64.7 |
| MS.gene27837.t1 | MTR_7g065265 | 37.624 | 101 | 59 | 4 | 15 | 114 | 123 | 220 | 5.38e-13 | 63.2 |
| MS.gene27837.t1 | MTR_7g065270 | 34.000 | 100 | 64 | 2 | 15 | 114 | 123 | 220 | 4.65e-12 | 60.5 |
| MS.gene27837.t1 | MTR_7g065230 | 32.039 | 103 | 63 | 3 | 15 | 117 | 123 | 218 | 9.22e-12 | 59.7 |
| MS.gene27837.t1 | MTR_7g065260 | 39.080 | 87 | 51 | 2 | 27 | 113 | 142 | 226 | 9.26e-12 | 60.1 |
| MS.gene27837.t1 | MTR_7g065710 | 31.633 | 98 | 61 | 3 | 9 | 106 | 114 | 205 | 1.16e-11 | 59.7 |
| MS.gene27837.t1 | MTR_7g065750 | 31.429 | 105 | 70 | 2 | 15 | 119 | 124 | 226 | 1.37e-11 | 59.3 |
| MS.gene27837.t1 | MTR_1g115195 | 33.333 | 105 | 68 | 2 | 11 | 114 | 116 | 219 | 1.73e-11 | 58.9 |
| MS.gene27837.t1 | MTR_5g040430 | 30.189 | 106 | 68 | 2 | 4 | 104 | 112 | 216 | 1.75e-11 | 58.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene27837.t1 | AT2G29460 | 36.522 | 115 | 69 | 3 | 5 | 117 | 112 | 224 | 3.76e-15 | 68.9 |
| MS.gene27837.t1 | AT2G29420 | 31.429 | 105 | 71 | 1 | 11 | 115 | 120 | 223 | 9.95e-14 | 65.1 |
| MS.gene27837.t1 | AT3G09270 | 37.778 | 90 | 55 | 1 | 15 | 104 | 123 | 211 | 1.32e-13 | 64.7 |
| MS.gene27837.t1 | AT1G59670 | 36.634 | 101 | 58 | 3 | 8 | 103 | 117 | 216 | 1.21e-12 | 62.4 |
| MS.gene27837.t1 | AT2G29490 | 37.736 | 106 | 61 | 4 | 1 | 104 | 109 | 211 | 1.65e-12 | 62.0 |
| MS.gene27837.t1 | AT2G29470 | 36.364 | 99 | 59 | 3 | 8 | 104 | 116 | 212 | 3.10e-12 | 61.2 |
| MS.gene27837.t1 | AT2G29480 | 33.333 | 114 | 72 | 3 | 6 | 117 | 113 | 224 | 3.40e-12 | 61.2 |
| MS.gene27837.t1 | AT1G10370 | 37.383 | 107 | 60 | 3 | 9 | 109 | 115 | 220 | 5.08e-12 | 60.5 |
| MS.gene27837.t1 | AT2G29450 | 31.304 | 115 | 74 | 4 | 6 | 117 | 112 | 224 | 4.51e-11 | 58.2 |
Find 39 sgRNAs with CRISPR-Local
Find 40 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTTCCAATTTGGGAAGAAAT+TGG | 0.111299 | 4.3:-13488583 | MS.gene27837:CDS |
| AAAGTTAGAAGAGGAGATAA+AGG | 0.273945 | 4.3:-13488674 | MS.gene27837:CDS |
| GACCCAATTTCTTCCCAAAT+TGG | 0.274164 | 4.3:+13488580 | None:intergenic |
| ATAAAGGGAAAGAAATTCTT+TGG | 0.305857 | 4.3:-13488658 | MS.gene27837:CDS |
| TATAGCTTGCGCTGGGTTCA+TGG | 0.334296 | 4.3:-13488762 | None:intergenic |
| AATGTCCTTCCATCTCTTCA+TGG | 0.338522 | 4.3:-13488534 | MS.gene27837:CDS |
| TTCCAATTTGGGAAGAAATT+GGG | 0.398944 | 4.3:-13488582 | MS.gene27837:CDS |
| TTATTGCCACCGTCGCATAA+AGG | 0.420018 | 4.3:-13488443 | MS.gene27837:CDS |
| ACCTTGATCTTGCTCTTGGA+TGG | 0.423990 | 4.3:-13488618 | MS.gene27837:CDS |
| TAAAGAATCATTTCATCCCT+TGG | 0.429286 | 4.3:+13488463 | None:intergenic |
| TGGATTGCATTGATTAAGAA+AGG | 0.435401 | 4.3:-13488742 | MS.gene27837:CDS |
| GGGTACCTTGATCTTGCTCT+TGG | 0.453072 | 4.3:-13488622 | MS.gene27837:CDS |
| TCTTTGGAGGGGACACTATC+GGG | 0.454808 | 4.3:-13488642 | MS.gene27837:CDS |
| TAAATTATTATAGCTTGCGC+TGG | 0.466026 | 4.3:-13488770 | None:intergenic |
| TGATTAAGAAAGGTGATGAA+TGG | 0.474122 | 4.3:-13488732 | MS.gene27837:CDS |
| AAAGGAGGCAAGAGAAATCA+TGG | 0.475218 | 4.3:-13488698 | MS.gene27837:CDS |
| TTCTTTGGAGGGGACACTAT+CGG | 0.475345 | 4.3:-13488643 | MS.gene27837:CDS |
| GATTAAGAAAGGTGATGAAT+GGG | 0.480475 | 4.3:-13488731 | MS.gene27837:CDS |
| AAGTTAGAAGAGGAGATAAA+GGG | 0.491500 | 4.3:-13488673 | MS.gene27837:CDS |
| GAAAGGTGATGAATGGGAAA+AGG | 0.495659 | 4.3:-13488725 | MS.gene27837:CDS |
| AAACACTCCTTGATGATGAT+AGG | 0.524604 | 4.3:+13488490 | None:intergenic |
| AATCATGGAAAAGTTAGAAG+AGG | 0.531445 | 4.3:-13488683 | MS.gene27837:CDS |
| AGGGAAAGAAATTCTTTGGA+GGG | 0.536580 | 4.3:-13488654 | MS.gene27837:CDS |
| ATTGATCTTCCATGAAGAGA+TGG | 0.543546 | 4.3:+13488525 | None:intergenic |
| TGAATGGGAAAAGGCTCTAA+AGG | 0.545680 | 4.3:-13488716 | MS.gene27837:CDS |
| TCCATCCAAGAGCAAGATCA+AGG | 0.557485 | 4.3:+13488617 | None:intergenic |
| CAAGGAGTGTTTGCCACCAA+GGG | 0.564625 | 4.3:-13488479 | MS.gene27837:CDS |
| TCAAGGAGTGTTTGCCACCA+AGG | 0.571195 | 4.3:-13488480 | MS.gene27837:CDS |
| ACTCCTTGATGATGATAGGA+TGG | 0.572678 | 4.3:+13488494 | None:intergenic |
| AAGGGAAAGAAATTCTTTGG+AGG | 0.576725 | 4.3:-13488655 | MS.gene27837:CDS |
| GAGCCATCCTATCATCATCA+AGG | 0.581782 | 4.3:-13488497 | MS.gene27837:CDS |
| AAATTATTATAGCTTGCGCT+GGG | 0.589664 | 4.3:-13488769 | None:intergenic |
| AAGAATACTCCTTTATGCGA+CGG | 0.595491 | 4.3:+13488434 | None:intergenic |
| GGGAAAGAAATTCTTTGGAG+GGG | 0.609810 | 4.3:-13488653 | MS.gene27837:CDS |
| ATCTTCCATGAAGAGATGGA+AGG | 0.621011 | 4.3:+13488529 | None:intergenic |
| AGAATCATTTCATCCCTTGG+TGG | 0.643379 | 4.3:+13488466 | None:intergenic |
| ATGGGAAAAGGCTCTAAAGG+AGG | 0.677413 | 4.3:-13488713 | MS.gene27837:CDS |
| AATACTCCTTTATGCGACGG+TGG | 0.688837 | 4.3:+13488437 | None:intergenic |
| GAGTATTCTTCAACTCATCG+TGG | 0.696267 | 4.3:-13488421 | MS.gene27837:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | ATAAAGGGAAAGAAATTCTT+TGG | - | chr4.3:13488516-13488535 | MS.gene27837:CDS | 25.0% |
| AAAGTTAGAAGAGGAGATAA+AGG | - | chr4.3:13488500-13488519 | MS.gene27837:CDS | 30.0% | |
| AAGTTAGAAGAGGAGATAAA+GGG | - | chr4.3:13488501-13488520 | MS.gene27837:CDS | 30.0% | |
| AATCATGGAAAAGTTAGAAG+AGG | - | chr4.3:13488491-13488510 | MS.gene27837:CDS | 30.0% | |
| GATTAAGAAAGGTGATGAAT+GGG | - | chr4.3:13488443-13488462 | MS.gene27837:CDS | 30.0% | |
| TAAAGAATCATTTCATCCCT+TGG | + | chr4.3:13488714-13488733 | None:intergenic | 30.0% | |
| TGATTAAGAAAGGTGATGAA+TGG | - | chr4.3:13488442-13488461 | MS.gene27837:CDS | 30.0% | |
| TTCCAATTTGGGAAGAAATT+GGG | - | chr4.3:13488592-13488611 | MS.gene27837:CDS | 30.0% | |
| !! | AGATGGAAGGACATTTTAAT+GGG | + | chr4.3:13488635-13488654 | None:intergenic | 30.0% |
| !! | CACTTGTTTTCTTCCAATTT+GGG | - | chr4.3:13488581-13488600 | MS.gene27837:CDS | 30.0% |
| !! | TCACTTGTTTTCTTCCAATT+TGG | - | chr4.3:13488580-13488599 | MS.gene27837:CDS | 30.0% |
| !! | TGGATTGCATTGATTAAGAA+AGG | - | chr4.3:13488432-13488451 | MS.gene27837:CDS | 30.0% |
| AAGAATACTCCTTTATGCGA+CGG | + | chr4.3:13488743-13488762 | None:intergenic | 35.0% | |
| AAGGGAAAGAAATTCTTTGG+AGG | - | chr4.3:13488519-13488538 | MS.gene27837:CDS | 35.0% | |
| AGGGAAAGAAATTCTTTGGA+GGG | - | chr4.3:13488520-13488539 | MS.gene27837:CDS | 35.0% | |
| ATTGATCTTCCATGAAGAGA+TGG | + | chr4.3:13488652-13488671 | None:intergenic | 35.0% | |
| CTTCCAATTTGGGAAGAAAT+TGG | - | chr4.3:13488591-13488610 | MS.gene27837:CDS | 35.0% | |
| ! | GAGATGGAAGGACATTTTAA+TGG | + | chr4.3:13488636-13488655 | None:intergenic | 35.0% |
| !! | AAACACTCCTTGATGATGAT+AGG | + | chr4.3:13488687-13488706 | None:intergenic | 35.0% |
| AAAGGAGGCAAGAGAAATCA+TGG | - | chr4.3:13488476-13488495 | MS.gene27837:CDS | 40.0% | |
| AATGTCCTTCCATCTCTTCA+TGG | - | chr4.3:13488640-13488659 | MS.gene27837:CDS | 40.0% | |
| AGAATCATTTCATCCCTTGG+TGG | + | chr4.3:13488711-13488730 | None:intergenic | 40.0% | |
| ATCTTCCATGAAGAGATGGA+AGG | + | chr4.3:13488648-13488667 | None:intergenic | 40.0% | |
| GAAAGGTGATGAATGGGAAA+AGG | - | chr4.3:13488449-13488468 | MS.gene27837:CDS | 40.0% | |
| GACCCAATTTCTTCCCAAAT+TGG | + | chr4.3:13488597-13488616 | None:intergenic | 40.0% | |
| GAGTATTCTTCAACTCATCG+TGG | - | chr4.3:13488753-13488772 | MS.gene27837:CDS | 40.0% | |
| ! | GGGAAAGAAATTCTTTGGAG+GGG | - | chr4.3:13488521-13488540 | MS.gene27837:CDS | 40.0% |
| ! | TGAATGGGAAAAGGCTCTAA+AGG | - | chr4.3:13488458-13488477 | MS.gene27837:CDS | 40.0% |
| !! | ACTCCTTGATGATGATAGGA+TGG | + | chr4.3:13488683-13488702 | None:intergenic | 40.0% |
| AATACTCCTTTATGCGACGG+TGG | + | chr4.3:13488740-13488759 | None:intergenic | 45.0% | |
| ACCTTGATCTTGCTCTTGGA+TGG | - | chr4.3:13488556-13488575 | MS.gene27837:CDS | 45.0% | |
| GAGCCATCCTATCATCATCA+AGG | - | chr4.3:13488677-13488696 | MS.gene27837:CDS | 45.0% | |
| TCCATCCAAGAGCAAGATCA+AGG | + | chr4.3:13488560-13488579 | None:intergenic | 45.0% | |
| TTATTGCCACCGTCGCATAA+AGG | - | chr4.3:13488731-13488750 | MS.gene27837:CDS | 45.0% | |
| ! | TTCTTTGGAGGGGACACTAT+CGG | - | chr4.3:13488531-13488550 | MS.gene27837:CDS | 45.0% |
| !! | ATGGGAAAAGGCTCTAAAGG+AGG | - | chr4.3:13488461-13488480 | MS.gene27837:CDS | 45.0% |
| GGGTACCTTGATCTTGCTCT+TGG | - | chr4.3:13488552-13488571 | MS.gene27837:CDS | 50.0% | |
| ! | TCTTTGGAGGGGACACTATC+GGG | - | chr4.3:13488532-13488551 | MS.gene27837:CDS | 50.0% |
| !! | CAAGGAGTGTTTGCCACCAA+GGG | - | chr4.3:13488695-13488714 | MS.gene27837:CDS | 50.0% |
| !! | TCAAGGAGTGTTTGCCACCA+AGG | - | chr4.3:13488694-13488713 | MS.gene27837:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.3 | gene | 13488417 | 13488779 | 13488417 | ID=MS.gene27837 |
| chr4.3 | mRNA | 13488417 | 13488779 | 13488417 | ID=MS.gene27837.t1;Parent=MS.gene27837 |
| chr4.3 | exon | 13488417 | 13488779 | 13488417 | ID=MS.gene27837.t1.exon1;Parent=MS.gene27837.t1 |
| chr4.3 | CDS | 13488417 | 13488779 | 13488417 | ID=cds.MS.gene27837.t1;Parent=MS.gene27837.t1 |
| Gene Sequence |
| Protein sequence |
