AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene27848

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene27848.t1 MTR_8g087540 97.257 401 11 0 1 401 1 401 0.0 814
MS.gene27848.t1 MTR_2g017890 40.633 411 186 9 1 383 2 382 6.88e-87 269
MS.gene27848.t1 MTR_6g043060 36.212 359 177 9 6 358 16 328 3.69e-63 206
MS.gene27848.t1 MTR_5g029680 51.630 184 82 1 11 187 31 214 1.13e-60 204
MS.gene27848.t1 MTR_8g083100 52.239 201 84 4 13 207 23 217 1.94e-60 203
MS.gene27848.t1 MTR_2g100680 49.751 201 92 2 12 207 31 227 3.18e-60 202
MS.gene27848.t1 MTR_2g100670 49.744 195 86 2 1 187 14 204 4.41e-54 182
MS.gene27848.t1 MTR_2g100670 49.744 195 86 2 1 187 122 312 5.61e-53 182
MS.gene27848.t1 MTR_1g102860 36.082 291 166 4 11 288 39 322 1.10e-52 179
MS.gene27848.t1 MTR_4g077970 44.865 185 94 2 10 187 10 193 1.50e-51 180
MS.gene27848.t1 MTR_7g095680 39.130 230 126 3 11 226 48 277 9.09e-49 169
MS.gene27848.t1 MTR_3g104550 41.584 202 114 1 11 208 45 246 1.09e-46 164
MS.gene27848.t1 MTR_1g061170 41.935 186 106 1 8 193 45 228 3.55e-46 165
MS.gene27848.t1 MTR_8g105780 46.875 192 91 3 12 194 22 211 1.12e-45 161
MS.gene27848.t1 MTR_1g071360 42.541 181 87 3 12 189 32 198 2.17e-43 154
MS.gene27848.t1 MTR_1g071360 42.541 181 87 3 12 189 29 195 2.86e-43 155
MS.gene27848.t1 MTR_2g043940 46.995 183 80 4 52 229 11 181 1.75e-41 150
MS.gene27848.t1 MTR_6g086805 38.308 201 116 2 10 206 9 205 4.38e-41 148
MS.gene27848.t1 MTR_5g010680 64.356 101 35 1 4 103 15 115 8.21e-41 148
MS.gene27848.t1 MTR_3g101870 64.646 99 34 1 6 103 17 115 2.77e-39 144
MS.gene27848.t1 MTR_5g089170 66.667 99 32 1 6 103 18 116 8.50e-39 140
MS.gene27848.t1 MTR_5g017470 58.824 102 42 0 8 109 5 106 1.00e-38 140
MS.gene27848.t1 MTR_1g100777 58.163 98 41 0 8 105 14 111 3.63e-37 137
MS.gene27848.t1 MTR_0020s0020 59.596 99 39 1 6 103 26 124 7.82e-36 134
MS.gene27848.t1 MTR_7g091370 49.123 114 58 0 3 116 13 126 2.74e-34 127
MS.gene27848.t1 MTR_4g088900 37.714 175 91 5 12 176 33 199 7.11e-30 115
MS.gene27848.t1 MTR_5g047190 53.061 98 39 2 20 110 1 98 1.86e-27 105
MS.gene27848.t1 MTR_4g045757 63.333 60 22 0 10 69 12 71 1.29e-21 88.6
MS.gene27848.t1 MTR_5g022830 87.805 41 5 0 361 401 40 80 5.13e-19 81.3
MS.gene27848.t1 MTR_6g044340 28.571 308 148 15 64 358 14 262 2.40e-17 81.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene27848.t1 AT4G18880 51.095 411 179 11 1 400 1 400 3.98e-115 342
MS.gene27848.t1 AT5G45710 49.492 394 145 8 1 391 1 343 2.30e-110 328
MS.gene27848.t1 AT5G45710 49.492 394 145 8 1 391 1 343 2.30e-110 328
MS.gene27848.t1 AT4G13980 48.500 200 94 2 13 207 24 219 2.28e-59 200
MS.gene27848.t1 AT1G32330 41.949 236 118 2 6 222 31 266 1.24e-58 198
MS.gene27848.t1 AT1G32330 41.949 236 118 2 6 222 31 266 1.53e-58 198
MS.gene27848.t1 AT5G45710 40.940 298 123 8 96 391 1 247 1.77e-55 183
MS.gene27848.t1 AT5G45710 40.940 298 123 8 96 391 1 247 1.77e-55 183
MS.gene27848.t1 AT5G16820 42.991 214 109 2 8 208 23 236 1.45e-54 187
MS.gene27848.t1 AT5G16820 42.991 214 109 2 8 208 23 236 1.45e-54 187
MS.gene27848.t1 AT4G17750 45.026 191 91 1 11 187 51 241 7.66e-54 186
MS.gene27848.t1 AT3G22830 41.256 223 117 2 8 216 57 279 1.92e-53 183
MS.gene27848.t1 AT3G02990 47.283 184 93 1 8 187 19 202 3.84e-53 184
MS.gene27848.t1 AT2G26150 41.262 206 113 1 11 208 43 248 2.28e-50 173
MS.gene27848.t1 AT2G26150 41.262 206 113 1 11 208 43 248 2.28e-50 173
MS.gene27848.t1 AT5G03720 45.810 179 89 2 10 185 53 226 7.43e-49 171
MS.gene27848.t1 AT5G03720 45.810 179 89 2 10 185 53 226 7.43e-49 171
MS.gene27848.t1 AT5G43840 43.000 200 90 3 11 208 16 193 1.36e-46 161
MS.gene27848.t1 AT1G67970 43.386 189 100 3 10 194 17 202 1.71e-45 161
MS.gene27848.t1 AT3G51910 45.652 184 85 3 11 194 28 196 4.30e-44 154
MS.gene27848.t1 AT5G54070 39.698 199 110 2 10 208 69 257 3.41e-41 148
MS.gene27848.t1 AT3G24520 39.247 186 86 4 10 195 15 173 1.69e-40 147
MS.gene27848.t1 AT1G46264 63.000 100 36 1 5 103 26 125 1.78e-39 144
MS.gene27848.t1 AT3G63350 38.725 204 111 3 1 204 21 210 1.90e-39 142
MS.gene27848.t1 AT5G62020 51.181 127 57 2 3 128 13 135 2.08e-38 140
MS.gene27848.t1 AT4G36990 63.265 98 35 1 9 105 10 107 9.05e-38 138
MS.gene27848.t1 AT4G11660 59.596 99 39 1 6 103 52 150 1.64e-36 137
MS.gene27848.t1 AT2G41690 58.491 106 43 1 11 115 39 144 2.19e-36 133
MS.gene27848.t1 AT4G18870 54.639 97 39 2 5 99 6 99 6.27e-27 109
MS.gene27848.t1 AT4G18870 52.174 92 44 0 8 99 142 233 2.05e-26 107
MS.gene27848.t1 AT1G77570 43.333 90 48 2 13 99 27 116 1.45e-17 79.3
MS.gene27848.t1 AT2G26150 33.621 116 77 0 72 187 49 164 1.14e-16 80.1
MS.gene27848.t1 AT2G26150 33.621 116 77 0 72 187 49 164 1.14e-16 80.1
MS.gene27848.t1 AT4G19630 38.947 95 55 1 8 99 7 101 2.15e-15 72.8

Find 85 sgRNAs with CRISPR-Local

Find 106 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGAGGCATCCGTGGCATTT+TGG 0.204494 4.1:+13158524 MS.gene27848:CDS
TCAACGTTCATGTCAATTTC+AGG 0.245219 4.1:-13158682 None:intergenic
AGCAAAACCCTTGCGAATTC+CGG 0.278319 4.1:-13157789 None:intergenic
ATATGTATGTCCAGGGATTT+AGG 0.286572 4.1:+13157975 MS.gene27848:intron
CACTCATTCAAACATGGATT+TGG 0.293073 4.1:+13158585 MS.gene27848:CDS
CTGGGGTTAATGATGTATTC+TGG 0.294292 4.1:+13158773 MS.gene27848:CDS
CTAACATGCCCCATCTGTTC+TGG 0.312124 4.1:-13158940 None:intergenic
CGAAGAGATACCAGCATGAT+TGG 0.339945 4.1:+13158209 MS.gene27848:CDS
CTAGTGATCATGGTAGATTT+TGG 0.344391 4.1:+13158890 MS.gene27848:CDS
AGAATCACCACTGCAGCAAC+TGG 0.356379 4.1:+13158754 MS.gene27848:CDS
AAGAGATTTACAGCAATCAT+AGG 0.368528 4.1:-13158334 None:intergenic
GACGTTAAACACCGAGAGAT+TGG 0.369357 4.1:+13158495 MS.gene27848:CDS
TGCCTTTCAGACTCCGTTAG+AGG 0.391700 4.1:-13158127 None:intergenic
GGAGAGAAAACGAAGGTTGC+CGG 0.399062 4.1:+13158375 MS.gene27848:CDS
ATTCAGCTGCCTGATAAAAC+TGG 0.407135 4.1:-13157841 None:intergenic
CTCTCCATGGCTTCTGCCAG+TGG 0.418903 4.1:-13158358 None:intergenic
TCATAAGTCTTTGCCAGAAA+AGG 0.419332 4.1:-13157690 None:intergenic
GCCTCAAGAGCAGCAGCAGC+TGG 0.427478 4.1:-13158706 None:intergenic
TCTGGCAAAGACTTATGAAA+TGG 0.438206 4.1:+13157695 MS.gene27848:CDS
TGAAATAGAGAAGCTGAAAC+AGG 0.448407 4.1:+13158162 MS.gene27848:CDS
GATGCACTCCACGAAACAAT+AGG 0.454683 4.1:-13157738 None:intergenic
TGGGGTTAATGATGTATTCT+GGG 0.456218 4.1:+13158774 MS.gene27848:CDS
ACCAGCTGCTGCTGCTCTTG+AGG 0.466489 4.1:+13158705 MS.gene27848:CDS
GCCTTTCAGACTCCGTTAGA+GGG 0.473627 4.1:-13158126 None:intergenic
ATGTCAATTTCAGGTGACTT+AGG 0.477806 4.1:-13158673 None:intergenic
TGCAGAGAATGACTATGAAC+GGG 0.482637 4.1:-13158085 None:intergenic
TCCTGGTGCATCGGAAGCAC+AGG 0.486616 4.1:+13158816 MS.gene27848:CDS
GAGACTCTTGCACATGAAGT+TGG 0.497289 4.1:+13158547 MS.gene27848:CDS
CGTTCACACTCATTCAAACA+TGG 0.502442 4.1:+13158579 MS.gene27848:CDS
GAAGGAAAACCTAGTGATCA+TGG 0.502730 4.1:+13158880 MS.gene27848:CDS
TTGGCAAATTCCCACTGCTC+CGG 0.502904 4.1:-13158007 None:intergenic
GGGATTTAGGAAAGTTGATC+CGG 0.507404 4.1:+13157988 MS.gene27848:intron
CTTGCCACTGGCAGAAGCCA+TGG 0.509175 4.1:+13158354 MS.gene27848:CDS
TTTCGTTGTGTGGAATCCTC+CGG 0.511600 4.1:+13157770 MS.gene27848:CDS
TCCTGTGCTTCCGATGCACC+AGG 0.511617 4.1:-13158817 None:intergenic
GAAGAGATACCAGCATGATT+GGG 0.512133 4.1:+13158210 MS.gene27848:CDS
TGCTGTAAATCTCTTGCCAC+TGG 0.515181 4.1:+13158342 MS.gene27848:CDS
GGAAAGTTGATCCGGAGCAG+TGG 0.515618 4.1:+13157996 MS.gene27848:CDS
CAGTTCTTGACAGAGGATCC+TGG 0.516203 4.1:+13158799 MS.gene27848:CDS
AGACTTCTGTAGCTTTGCCC+AGG 0.516910 4.1:+13158446 MS.gene27848:CDS
AAACAATCATCCAGAACAGA+TGG 0.518388 4.1:+13158930 MS.gene27848:CDS
CCTCGGATCCTATTGTTTCG+TGG 0.527360 4.1:+13157730 MS.gene27848:CDS
TTATTGTGCTTAAAGAATCT+CGG 0.532647 4.1:-13157813 None:intergenic
CGAACAAAAGTTTCGTTGTG+TGG 0.533195 4.1:+13157760 MS.gene27848:CDS
GCATTGTTCGAAAGATCAGT+TGG 0.538254 4.1:+13158252 MS.gene27848:CDS
GTCAAAGTAGAGTTATCCTC+TGG 0.546841 4.1:-13158475 None:intergenic
AGCTAGTGTTGAAGATGCTA+TGG 0.547413 4.1:+13158423 MS.gene27848:CDS
CTCAAGTTGATCCAATCTCT+CGG 0.553126 4.1:-13158506 None:intergenic
GACAGAGGATCCTGGTGCAT+CGG 0.553354 4.1:+13158807 MS.gene27848:CDS
ATTAACCCCAGTTGCTGCAG+TGG 0.553513 4.1:-13158761 None:intergenic
CCCCTCTAACGGAGTCTGAA+AGG 0.554429 4.1:+13158125 MS.gene27848:CDS
ATGCAGAGAATGACTATGAA+CGG 0.560960 4.1:-13158086 None:intergenic
GCTGCAGTGGTGATTCTCGC+AGG 0.569378 4.1:-13158748 None:intergenic
TAAGTCTTTGCCAGAAAAGG+AGG 0.570699 4.1:-13157687 None:intergenic
CAAAATCTACCATGATCACT+AGG 0.571873 4.1:-13158889 None:intergenic
AACAATCATCCAGAACAGAT+GGG 0.574845 4.1:+13158931 MS.gene27848:CDS
GGAATCCTCCGGAATTCGCA+AGG 0.575730 4.1:+13157781 MS.gene27848:CDS
TTAAAGCAACCACTTCTCGC+CGG 0.577237 4.1:-13158394 None:intergenic
GAAAGTTGATCCGGAGCAGT+GGG 0.578322 4.1:+13157997 MS.gene27848:CDS
CATGCCTTTCCCAATCATGC+TGG 0.579073 4.1:-13158219 None:intergenic
GAATCACCACTGCAGCAACT+GGG 0.580374 4.1:+13158755 MS.gene27848:CDS
CGAAGGTTGCCGGCGAGAAG+TGG 0.586677 4.1:+13158385 MS.gene27848:CDS
CCTTTCAGACTCCGTTAGAG+GGG 0.598591 4.1:-13158125 None:intergenic
TGAATATTCTTCATCAGATG+CGG 0.602613 4.1:-13158052 None:intergenic
GACTTCTGTAGCTTTGCCCA+GGG 0.603526 4.1:+13158447 MS.gene27848:CDS
GATACCAGCATGATTGGGAA+AGG 0.606022 4.1:+13158215 MS.gene27848:CDS
AAAACCCTTGCGAATTCCGG+AGG 0.607238 4.1:-13157786 None:intergenic
TCAAGCACAAGCCCCTCTAA+CGG 0.613167 4.1:+13158114 MS.gene27848:CDS
GGCAAAGACTTATGAAATGG+TGG 0.613237 4.1:+13157698 MS.gene27848:CDS
GGATCAACTTGAGGCATCCG+TGG 0.615719 4.1:+13158516 MS.gene27848:CDS
CGAGAGATTGGATCAACTTG+AGG 0.627773 4.1:+13158507 MS.gene27848:CDS
AATGGTGGATGATCGATCCT+CGG 0.628991 4.1:+13157713 MS.gene27848:CDS
GAATCCTCCGGAATTCGCAA+GGG 0.629052 4.1:+13157782 MS.gene27848:CDS
TGATGAAGAATATTCATAGA+CGG 0.629516 4.1:+13158059 MS.gene27848:CDS
CTGCAATGCTTCAGAAACAG+AGG 0.634333 4.1:-13158308 None:intergenic
ATATCTTGTCAGCAACTAGA+CGG 0.636770 4.1:+13158640 MS.gene27848:CDS
ACAATCATCCAGAACAGATG+GGG 0.647372 4.1:+13158932 MS.gene27848:CDS
CTGGGAACAGTTCTTGACAG+AGG 0.655854 4.1:+13158792 MS.gene27848:CDS
AAGAGTCTCCCAAAATGCCA+CGG 0.657362 4.1:-13158533 None:intergenic
AATCACCACTGCAGCAACTG+GGG 0.658502 4.1:+13158756 MS.gene27848:CDS
GGATAGAGAACAACTCCTCG+TGG 0.661247 4.1:+13158183 MS.gene27848:CDS
CCACGAAACAATAGGATCCG+AGG 0.666703 4.1:-13157730 None:intergenic
GGTATCTCTTCGTCTCCACG+AGG 0.715796 4.1:-13158198 None:intergenic
TTTGCCCAGGGAAAATCCAG+AGG 0.715905 4.1:+13158459 MS.gene27848:CDS
AAGCCATGGAGAGAAAACGA+AGG 0.761326 4.1:+13158368 MS.gene27848:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTATTTTATTTACTGATTC+TGG + chr4.1:13157924-13157943 MS.gene27848:intron 15.0%
! AATAGTAGCAGAAAAAATGA+AGG + chr4.1:13158862-13158881 MS.gene27848:CDS 25.0%
! TATGTAAGCAAATTTGATGT+CGG + chr4.1:13157867-13157886 MS.gene27848:intron 25.0%
! TGATGAAGAATATTCATAGA+CGG + chr4.1:13158059-13158078 MS.gene27848:CDS 25.0%
! TTATTGTGCTTAAAGAATCT+CGG - chr4.1:13157816-13157835 None:intergenic 25.0%
!! TGCCAATGATGATTTTATAA+GGG + chr4.1:13158024-13158043 MS.gene27848:CDS 25.0%
!! TTGCCAATGATGATTTTATA+AGG + chr4.1:13158023-13158042 MS.gene27848:CDS 25.0%
!!! TATTTACTGATTCTGGTTTT+TGG + chr4.1:13157931-13157950 MS.gene27848:intron 25.0%
AAGAGATTTACAGCAATCAT+AGG - chr4.1:13158337-13158356 None:intergenic 30.0%
GAAAGATCAGTTGGAAAAAT+TGG + chr4.1:13158261-13158280 MS.gene27848:CDS 30.0%
TCATAGTATATGTATGTCCA+GGG + chr4.1:13157968-13157987 MS.gene27848:intron 30.0%
TGAATATTCTTCATCAGATG+CGG - chr4.1:13158055-13158074 None:intergenic 30.0%
TTCATAGTATATGTATGTCC+AGG + chr4.1:13157967-13157986 MS.gene27848:intron 30.0%
! CCAATGATGATTTTATAAGG+GGG + chr4.1:13158026-13158045 MS.gene27848:CDS 30.0%
! GCCAATGATGATTTTATAAG+GGG + chr4.1:13158025-13158044 MS.gene27848:CDS 30.0%
!! ATAACTTCTCCAGTTTTATC+AGG + chr4.1:13157832-13157851 MS.gene27848:CDS 30.0%
AAACAATCATCCAGAACAGA+TGG + chr4.1:13158930-13158949 MS.gene27848:CDS 35.0%
AACAATCATCCAGAACAGAT+GGG + chr4.1:13158931-13158950 MS.gene27848:CDS 35.0%
ATATCTTGTCAGCAACTAGA+CGG + chr4.1:13158640-13158659 MS.gene27848:CDS 35.0%
ATATGTATGTCCAGGGATTT+AGG + chr4.1:13157975-13157994 MS.gene27848:intron 35.0%
ATGCAGAGAATGACTATGAA+CGG - chr4.1:13158089-13158108 None:intergenic 35.0%
CAAAATCTACCATGATCACT+AGG - chr4.1:13158892-13158911 None:intergenic 35.0%
CACTCATTCAAACATGGATT+TGG + chr4.1:13158585-13158604 MS.gene27848:CDS 35.0%
CCCCCTTATAAAATCATCAT+TGG - chr4.1:13158029-13158048 None:intergenic 35.0%
TCAACGTTCATGTCAATTTC+AGG - chr4.1:13158685-13158704 None:intergenic 35.0%
TCATAAGTCTTTGCCAGAAA+AGG - chr4.1:13157693-13157712 None:intergenic 35.0%
TCTGGCAAAGACTTATGAAA+TGG + chr4.1:13157695-13157714 MS.gene27848:CDS 35.0%
TGAAATAGAGAAGCTGAAAC+AGG + chr4.1:13158162-13158181 MS.gene27848:CDS 35.0%
TGGGGTTAATGATGTATTCT+GGG + chr4.1:13158774-13158793 MS.gene27848:CDS 35.0%
! ATGTCAATTTCAGGTGACTT+AGG - chr4.1:13158676-13158695 None:intergenic 35.0%
! CTAGTGATCATGGTAGATTT+TGG + chr4.1:13158890-13158909 MS.gene27848:CDS 35.0%
ACAATCATCCAGAACAGATG+GGG + chr4.1:13158932-13158951 MS.gene27848:CDS 40.0%
ATTCAGCTGCCTGATAAAAC+TGG - chr4.1:13157844-13157863 None:intergenic 40.0%
CGAACAAAAGTTTCGTTGTG+TGG + chr4.1:13157760-13157779 MS.gene27848:CDS 40.0%
CGTTCACACTCATTCAAACA+TGG + chr4.1:13158579-13158598 MS.gene27848:CDS 40.0%
CTGGGGTTAATGATGTATTC+TGG + chr4.1:13158773-13158792 MS.gene27848:CDS 40.0%
GAAGAGATACCAGCATGATT+GGG + chr4.1:13158210-13158229 MS.gene27848:CDS 40.0%
GAAGGAAAACCTAGTGATCA+TGG + chr4.1:13158880-13158899 MS.gene27848:CDS 40.0%
GATCAACTTTCCTAAATCCC+TGG - chr4.1:13157988-13158007 None:intergenic 40.0%
GCATTGTTCGAAAGATCAGT+TGG + chr4.1:13158252-13158271 MS.gene27848:CDS 40.0%
GGCAAAGACTTATGAAATGG+TGG + chr4.1:13157698-13157717 MS.gene27848:CDS 40.0%
GTCAAAGTAGAGTTATCCTC+TGG - chr4.1:13158478-13158497 None:intergenic 40.0%
TAAGTCTTTGCCAGAAAAGG+AGG - chr4.1:13157690-13157709 None:intergenic 40.0%
TGCAGAGAATGACTATGAAC+GGG - chr4.1:13158088-13158107 None:intergenic 40.0%
! AGCTAGTGTTGAAGATGCTA+TGG + chr4.1:13158423-13158442 MS.gene27848:CDS 40.0%
! GGGATTTAGGAAAGTTGATC+CGG + chr4.1:13157988-13158007 MS.gene27848:intron 40.0%
! GTGATCATGGTAGATTTTGG+TGG + chr4.1:13158893-13158912 MS.gene27848:CDS 40.0%
! TTATCCTCTGGATTTTCCCT+GGG - chr4.1:13158466-13158485 None:intergenic 40.0%
!! CTCAAGTTGATCCAATCTCT+CGG - chr4.1:13158509-13158528 None:intergenic 40.0%
!! GTAGATTTTGGTGGAACATG+CGG + chr4.1:13158902-13158921 MS.gene27848:CDS 40.0%
AAGAGTCTCCCAAAATGCCA+CGG - chr4.1:13158536-13158555 None:intergenic 45.0%
AAGCCATGGAGAGAAAACGA+AGG + chr4.1:13158368-13158387 MS.gene27848:CDS 45.0%
AGCAAAACCCTTGCGAATTC+CGG - chr4.1:13157792-13157811 None:intergenic 45.0%
CGAAGAGATACCAGCATGAT+TGG + chr4.1:13158209-13158228 MS.gene27848:CDS 45.0%
CGAGAGATTGGATCAACTTG+AGG + chr4.1:13158507-13158526 MS.gene27848:CDS 45.0%
CTGCAATGCTTCAGAAACAG+AGG - chr4.1:13158311-13158330 None:intergenic 45.0%
GACGTTAAACACCGAGAGAT+TGG + chr4.1:13158495-13158514 MS.gene27848:CDS 45.0%
GAGACTCTTGCACATGAAGT+TGG + chr4.1:13158547-13158566 MS.gene27848:CDS 45.0%
GATACCAGCATGATTGGGAA+AGG + chr4.1:13158215-13158234 MS.gene27848:CDS 45.0%
GATGCACTCCACGAAACAAT+AGG - chr4.1:13157741-13157760 None:intergenic 45.0%
TGCTGTAAATCTCTTGCCAC+TGG + chr4.1:13158342-13158361 MS.gene27848:CDS 45.0%
TTAAAGCAACCACTTCTCGC+CGG - chr4.1:13158397-13158416 None:intergenic 45.0%
TTTCGTTGTGTGGAATCCTC+CGG + chr4.1:13157770-13157789 MS.gene27848:CDS 45.0%
! AAGTTCTCTGCCTCCTTTTC+TGG + chr4.1:13157677-13157696 MS.gene27848:CDS 45.0%
! CAACCTTCGTTTTCTCTCCA+TGG - chr4.1:13158374-13158393 None:intergenic 45.0%
! GTTATCCTCTGGATTTTCCC+TGG - chr4.1:13158467-13158486 None:intergenic 45.0%
!! AATGGTGGATGATCGATCCT+CGG + chr4.1:13157713-13157732 MS.gene27848:CDS 45.0%
AAAACCCTTGCGAATTCCGG+AGG - chr4.1:13157789-13157808 None:intergenic 50.0%
AATCACCACTGCAGCAACTG+GGG + chr4.1:13158756-13158775 MS.gene27848:CDS 50.0%
AGAATCACCACTGCAGCAAC+TGG + chr4.1:13158754-13158773 MS.gene27848:CDS 50.0%
ATTAACCCCAGTTGCTGCAG+TGG - chr4.1:13158764-13158783 None:intergenic 50.0%
CAGTTCTTGACAGAGGATCC+TGG + chr4.1:13158799-13158818 MS.gene27848:CDS 50.0%
CATGCCTTTCCCAATCATGC+TGG - chr4.1:13158222-13158241 None:intergenic 50.0%
CCACGAAACAATAGGATCCG+AGG - chr4.1:13157733-13157752 None:intergenic 50.0%
CCTCGGATCCTATTGTTTCG+TGG + chr4.1:13157730-13157749 MS.gene27848:CDS 50.0%
CCTTTCAGACTCCGTTAGAG+GGG - chr4.1:13158128-13158147 None:intergenic 50.0%
CTAACATGCCCCATCTGTTC+TGG - chr4.1:13158943-13158962 None:intergenic 50.0%
CTGGGAACAGTTCTTGACAG+AGG + chr4.1:13158792-13158811 MS.gene27848:CDS 50.0%
GAATCACCACTGCAGCAACT+GGG + chr4.1:13158755-13158774 MS.gene27848:CDS 50.0%
GAATCCTCCGGAATTCGCAA+GGG + chr4.1:13157782-13157801 MS.gene27848:CDS 50.0%
GCCTTTCAGACTCCGTTAGA+GGG - chr4.1:13158129-13158148 None:intergenic 50.0%
GGAGAGAAAACGAAGGTTGC+CGG + chr4.1:13158375-13158394 MS.gene27848:CDS 50.0%
GGATAGAGAACAACTCCTCG+TGG + chr4.1:13158183-13158202 MS.gene27848:CDS 50.0%
TCAAGCACAAGCCCCTCTAA+CGG + chr4.1:13158114-13158133 MS.gene27848:CDS 50.0%
TGCCTTTCAGACTCCGTTAG+AGG - chr4.1:13158130-13158149 None:intergenic 50.0%
TTGGCAAATTCCCACTGCTC+CGG - chr4.1:13158010-13158029 None:intergenic 50.0%
TTTGCCCAGGGAAAATCCAG+AGG + chr4.1:13158459-13158478 MS.gene27848:CDS 50.0%
! AGACTTCTGTAGCTTTGCCC+AGG + chr4.1:13158446-13158465 MS.gene27848:CDS 50.0%
! GAAAGTTGATCCGGAGCAGT+GGG + chr4.1:13157997-13158016 MS.gene27848:CDS 50.0%
! GACTTCTGTAGCTTTGCCCA+GGG + chr4.1:13158447-13158466 MS.gene27848:CDS 50.0%
!! TGAGGCATCCGTGGCATTTT+GGG + chr4.1:13158525-13158544 MS.gene27848:CDS 50.0%
!! TTGAGGCATCCGTGGCATTT+TGG + chr4.1:13158524-13158543 MS.gene27848:CDS 50.0%
CCCCTCTAACGGAGTCTGAA+AGG + chr4.1:13158125-13158144 MS.gene27848:CDS 55.0%
GACAGAGGATCCTGGTGCAT+CGG + chr4.1:13158807-13158826 MS.gene27848:CDS 55.0%
GGAATCCTCCGGAATTCGCA+AGG + chr4.1:13157781-13157800 MS.gene27848:CDS 55.0%
GGTATCTCTTCGTCTCCACG+AGG - chr4.1:13158201-13158220 None:intergenic 55.0%
! GGAAAGTTGATCCGGAGCAG+TGG + chr4.1:13157996-13158015 MS.gene27848:CDS 55.0%
! GGATCAACTTGAGGCATCCG+TGG + chr4.1:13158516-13158535 MS.gene27848:CDS 55.0%
ACCAGCTGCTGCTGCTCTTG+AGG + chr4.1:13158705-13158724 MS.gene27848:CDS 60.0%
CTCTCCATGGCTTCTGCCAG+TGG - chr4.1:13158361-13158380 None:intergenic 60.0%
CTTGCCACTGGCAGAAGCCA+TGG + chr4.1:13158354-13158373 MS.gene27848:CDS 60.0%
GCTGCAGTGGTGATTCTCGC+AGG - chr4.1:13158751-13158770 None:intergenic 60.0%
TCCTGTGCTTCCGATGCACC+AGG - chr4.1:13158820-13158839 None:intergenic 60.0%
! TCCTGGTGCATCGGAAGCAC+AGG + chr4.1:13158816-13158835 MS.gene27848:CDS 60.0%
CGAAGGTTGCCGGCGAGAAG+TGG + chr4.1:13158385-13158404 MS.gene27848:CDS 65.0%
GCCTCAAGAGCAGCAGCAGC+TGG - chr4.1:13158709-13158728 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr4.1 gene 13157657 13158981 13157657 ID=MS.gene27848
chr4.1 mRNA 13157657 13158981 13157657 ID=MS.gene27848.t1;Parent=MS.gene27848
chr4.1 exon 13157657 13157869 13157657 ID=MS.gene27848.t1.exon1;Parent=MS.gene27848.t1
chr4.1 CDS 13157657 13157869 13157657 ID=cds.MS.gene27848.t1;Parent=MS.gene27848.t1
chr4.1 exon 13157989 13158981 13157989 ID=MS.gene27848.t1.exon2;Parent=MS.gene27848.t1
chr4.1 CDS 13157989 13158981 13157989 ID=cds.MS.gene27848.t1;Parent=MS.gene27848.t1
Gene Sequence

>MS.gene27848

ATGGATGAAGCTGGAAGTTCAAGTTCTCTGCCTCCTTTTCTGGCAAAGACTTATGAAATGGTGGATGATCGATCCTCGGATCCTATTGTTTCGTGGAGTGCATCGAACAAAAGTTTCGTTGTGTGGAATCCTCCGGAATTCGCAAGGGTTTTGCTTCCGAGATTCTTTAAGCACAATAACTTCTCCAGTTTTATCAGGCAGCTGAATACATATGTAAGCAAATTTGATGTCGGTGTTGTATTTGTTGATGCGAATTCTTGTTTTATGTTTATTTTATTTACTGATTCTGGTTTTTGGTTGCTTGAACTGATTCATAGTATATGTATGTCCAGGGATTTAGGAAAGTTGATCCGGAGCAGTGGGAATTTGCCAATGATGATTTTATAAGGGGGCAACCGCATCTGATGAAGAATATTCATAGACGGAAGCCCGTTCATAGTCATTCTCTGCATAATCTTCAAGCACAAGCCCCTCTAACGGAGTCTGAAAGGCAGAGTATGATTGATGAAATAGAGAAGCTGAAACAGGATAGAGAACAACTCCTCGTGGAGACGAAGAGATACCAGCATGATTGGGAAAGGCATGAGATTCAAATGCATTGTTCGAAAGATCAGTTGGAAAAATTGGAACATAAACAACAAAAGATGTTGTCCTCTGTTTCTGAAGCATTGCAGAAACCTATGATTGCTGTAAATCTCTTGCCACTGGCAGAAGCCATGGAGAGAAAACGAAGGTTGCCGGCGAGAAGTGGTTGCTTTAATAATGAAGCTAGTGTTGAAGATGCTATGGAGACTTCTGTAGCTTTGCCCAGGGAAAATCCAGAGGATAACTCTACTTTGACGTTAAACACCGAGAGATTGGATCAACTTGAGGCATCCGTGGCATTTTGGGAGACTCTTGCACATGAAGTTGGCGACAATTTCGTTCACACTCATTCAAACATGGATTTGGATGAATCTACATGCTGTGCTGATAGTTTGTCGATATCTTGTCAGCAACTAGACGGTGAAGTTCGACCTAAGTCACCTGAAATTGACATGAACGTTGAACCAGCTGCTGCTGCTCTTGAGGCTGTTGCAGTAAAAGAACAACCTGCGAGAATCACCACTGCAGCAACTGGGGTTAATGATGTATTCTGGGAACAGTTCTTGACAGAGGATCCTGGTGCATCGGAAGCACAGGAAGTGCAATCAGAAAGAAAAGATAATAGTAGCAGAAAAAATGAAGGAAAACCTAGTGATCATGGTAGATTTTGGTGGAACATGCGGAAATCAAACAATCATCCAGAACAGATGGGGCATGTTAGTCAAGTAGAGAAAATTTAG
Protein sequence

>MS.gene27848.t1

MDEAGSSSSLPPFLAKTYEMVDDRSSDPIVSWSASNKSFVVWNPPEFARVLLPRFFKHNNFSSFIRQLNTYGFRKVDPEQWEFANDDFIRGQPHLMKNIHRRKPVHSHSLHNLQAQAPLTESERQSMIDEIEKLKQDREQLLVETKRYQHDWERHEIQMHCSKDQLEKLEHKQQKMLSSVSEALQKPMIAVNLLPLAEAMERKRRLPARSGCFNNEASVEDAMETSVALPRENPEDNSTLTLNTERLDQLEASVAFWETLAHEVGDNFVHTHSNMDLDESTCCADSLSISCQQLDGEVRPKSPEIDMNVEPAAAALEAVAVKEQPARITTAATGVNDVFWEQFLTEDPGASEAQEVQSERKDNSSRKNEGKPSDHGRFWWNMRKSNNHPEQMGHVSQVEKI