AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene27862

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene27862.t1 MTR_8g087770 86.451 679 86 3 1 678 1 674 0.0 1195
MS.gene27862.t1 MTR_8g087750 82.712 671 111 3 6 675 11 677 0.0 1131
MS.gene27862.t1 MTR_8g087763 88.889 225 25 0 374 598 4 228 1.21e-144 425
MS.gene27862.t1 MTR_4g093750 34.491 403 235 14 67 452 35 425 2.83e-42 164
MS.gene27862.t1 MTR_4g093750 40.210 286 149 9 67 337 35 313 2.20e-41 161
MS.gene27862.t1 MTR_2g009690 37.261 314 185 6 63 366 40 351 5.56e-41 160
MS.gene27862.t1 MTR_5g009150 30.128 468 291 15 69 518 35 484 2.06e-34 139
MS.gene27862.t1 MTR_1g032550 35.336 283 163 7 69 337 35 311 2.34e-33 136
MS.gene27862.t1 MTR_8g105580 35.461 282 163 6 69 337 35 310 7.45e-33 134
MS.gene27862.t1 MTR_8g105580 35.461 282 163 6 69 337 35 310 7.97e-33 134
MS.gene27862.t1 MTR_7g451010 34.700 317 181 9 69 369 40 346 6.45e-32 132
MS.gene27862.t1 MTR_8g099550 33.956 321 178 10 69 369 34 340 6.80e-32 131
MS.gene27862.t1 MTR_8g009510 35.220 318 178 10 69 369 40 346 1.05e-31 131
MS.gene27862.t1 MTR_4g098920 34.375 320 178 11 69 369 34 340 3.69e-31 129
MS.gene27862.t1 MTR_8g027675 41.709 199 105 5 69 264 34 224 6.03e-31 129
MS.gene27862.t1 MTR_4g085860 75.000 76 18 1 206 280 2 77 2.37e-30 114
MS.gene27862.t1 MTR_6g045107 73.684 76 19 1 206 280 2 77 2.07e-28 108
MS.gene27862.t1 MTR_8g009510 31.452 248 146 7 136 369 2 239 3.36e-17 85.9
MS.gene27862.t1 MTR_8g463270 30.131 229 145 6 54 269 27 253 4.04e-16 82.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene27862.t1 AT1G60500 56.994 672 280 5 3 671 2 667 0.0 774
MS.gene27862.t1 AT1G60530 71.379 290 75 1 18 307 19 300 7.04e-143 419
MS.gene27862.t1 AT2G14120 29.955 444 288 10 25 452 1 437 2.07e-39 155
MS.gene27862.t1 AT2G14120 29.955 444 288 10 25 452 1 437 2.45e-39 155
MS.gene27862.t1 AT2G14120 29.955 444 288 10 25 452 1 437 2.81e-39 155
MS.gene27862.t1 AT2G14120 33.828 337 205 6 25 346 1 334 3.40e-39 155
MS.gene27862.t1 AT4G33650 29.571 443 268 11 35 452 29 452 5.03e-39 154
MS.gene27862.t1 AT4G33650 29.571 443 268 11 35 452 29 452 6.12e-39 154
MS.gene27862.t1 AT1G14830 32.903 310 196 6 69 369 35 341 5.06e-34 138
MS.gene27862.t1 AT5G42080 30.258 466 293 13 69 518 34 483 5.05e-33 135
MS.gene27862.t1 AT5G42080 30.258 466 293 13 69 518 34 483 5.05e-33 135
MS.gene27862.t1 AT2G44590 24.307 613 406 19 69 665 35 605 6.09e-33 135
MS.gene27862.t1 AT3G60190 33.232 328 199 9 69 386 40 357 9.20e-33 134
MS.gene27862.t1 AT5G42080 34.505 313 187 8 69 369 34 340 3.67e-32 130
MS.gene27862.t1 AT5G42080 34.505 313 187 8 69 369 34 340 8.36e-32 131
MS.gene27862.t1 AT3G61760 34.472 322 175 12 69 369 34 340 3.20e-30 125
MS.gene27862.t1 AT3G61760 34.472 322 175 12 69 369 34 340 3.31e-30 126
MS.gene27862.t1 AT3G61760 34.472 322 175 12 69 369 34 340 4.29e-30 125
MS.gene27862.t1 AT2G44590 22.838 613 398 20 69 665 35 588 3.43e-23 104
MS.gene27862.t1 AT2G44590 28.875 329 195 10 69 386 35 335 2.46e-22 102
MS.gene27862.t1 AT1G53140 27.542 236 159 5 44 269 36 269 5.03e-15 79.3

Find 126 sgRNAs with CRISPR-Local

Find 134 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACACTTTCAACAAAATTAAT+TGG 0.173861 4.1:-12967784 None:intergenic
CTTGAGGAAGCCAAATTTAT+TGG 0.191059 4.1:+12967688 MS.gene27862:CDS
ATTCGGAAGACCAATAAATT+TGG 0.194065 4.1:-12967698 None:intergenic
ATGGACAGGATTGGAAATTA+TGG 0.215755 4.1:+12968414 MS.gene27862:CDS
ATCAAGTTTGTTTGCCTTTG+TGG 0.244719 4.1:-12966432 None:intergenic
TATTGTTGGAATTCCTGTTT+TGG 0.276334 4.1:+12967318 MS.gene27862:CDS
GAGGAGTCTCCTTCAATATC+AGG 0.303692 4.1:+12968324 MS.gene27862:CDS
ATCTGATCATAAATGTTATC+AGG 0.304162 4.1:-12966971 None:intergenic
AGGCCAGATTGACAGCTTAT+TGG 0.313028 4.1:+12968160 MS.gene27862:CDS
TTCTAACTATTATCAGCTTC+AGG 0.313313 4.1:+12967855 MS.gene27862:CDS
GAACTTTGATTTCCTCCATT+AGG 0.316135 4.1:-12967666 None:intergenic
TAACATTTATGATCAGATTA+AGG 0.317964 4.1:+12966976 MS.gene27862:CDS
CTTCCAACCATTGTTGTTGT+TGG 0.322456 4.1:+12966614 MS.gene27862:CDS
AGAGACCGTGGCCAACATTA+TGG 0.325375 4.1:+12968395 MS.gene27862:CDS
TCACAAACTGTTGATAAAAC+TGG 0.334047 4.1:+12967094 MS.gene27862:CDS
TCCACAGCCAGAGCTTGTTT+TGG 0.338480 4.1:+12966754 MS.gene27862:CDS
AATTCAGCATATCTACTAAT+CGG 0.340774 4.1:-12967591 None:intergenic
CCTTACTTCAGAGAAAAGTT+AGG 0.351453 4.1:+12967746 MS.gene27862:CDS
TGAAAGTGTATGGAATTATC+TGG 0.352378 4.1:+12967798 MS.gene27862:CDS
CTTACTATGGTTGATCTTCC+AGG 0.352429 4.1:+12966920 MS.gene27862:CDS
TAAGAAGATTAATGAGAAAC+TGG 0.360082 4.1:+12967399 MS.gene27862:CDS
CAAGGGTACCTTTGGTTATG+AGG 0.366182 4.1:+12966714 MS.gene27862:CDS
TGGACAGGATTGGAAATTAT+GGG 0.366857 4.1:+12968415 MS.gene27862:CDS
CACATGTAACCCTGAGTACT+TGG 0.369075 4.1:+12967978 MS.gene27862:CDS
GCAGTTCTTGGCATGAAATT+CGG 0.376721 4.1:-12967715 None:intergenic
GCCTGCTAATTTATCCTCAC+TGG 0.379029 4.1:+12967450 MS.gene27862:CDS
TGGAGTTCCTGATCTTACTA+TGG 0.379945 4.1:+12966907 MS.gene27862:CDS
CTCCATTAGGAAATCCTTAG+TGG 0.385218 4.1:-12967653 None:intergenic
TAAATCTCCTGAAGGTTTGC+TGG 0.387244 4.1:+12967150 MS.gene27862:CDS
TTCAAAATAATACTCTCTTC+AGG 0.392099 4.1:-12967022 None:intergenic
GCTCTGGCTGTGGAAGTAAA+TGG 0.394731 4.1:-12966745 None:intergenic
AACAATAGATTTGTCTATCC+TGG 0.395168 4.1:-12967302 None:intergenic
ATAACTCGTGTTCCTGTTCA+TGG 0.396246 4.1:+12966944 MS.gene27862:CDS
ACCTTCTCCAGCAAACCTTC+AGG 0.399453 4.1:-12967157 None:intergenic
TTTCCAATAAGCTGTCAATC+TGG 0.399717 4.1:-12968163 None:intergenic
TTACTACTTGTGAGTCGATA+AGG 0.405010 4.1:+12967068 MS.gene27862:CDS
TGTCTCTTCCCTGATATTGA+AGG 0.410432 4.1:-12968333 None:intergenic
TTAATCTTCTTAATGATCTC+TGG 0.413053 4.1:-12967388 None:intergenic
GACGATGTTAACATTGGTCT+TGG 0.414913 4.1:+12967187 MS.gene27862:CDS
TTGATGCTCTTGAAAATCTA+AGG 0.416842 4.1:+12966561 MS.gene27862:CDS
AATTTGTACAAGGGTACCTT+TGG 0.424069 4.1:+12966706 MS.gene27862:CDS
AAGGTCAGAAAAGCAGTTCT+TGG 0.424321 4.1:-12967727 None:intergenic
GTCTTGGTTATGTTTGTGTA+AGG 0.435750 4.1:+12967203 MS.gene27862:CDS
ATATCCACACATGTTGAATC+AGG 0.440980 4.1:+12968125 MS.gene27862:CDS
ACAGCCACTGAGGAGCTTGC+TGG 0.443000 4.1:+12966833 MS.gene27862:CDS
TGGGGTGTTGGAAATCCCTT+TGG 0.443439 4.1:-12966861 None:intergenic
CTTACTTCAGAGAAAAGTTA+GGG 0.447328 4.1:+12967747 MS.gene27862:CDS
GGCTACTAGTTTGCATGAGC+TGG 0.448592 4.1:+12967420 MS.gene27862:CDS
AGGATAGACAAATCTATTGT+TGG 0.453655 4.1:+12967304 MS.gene27862:CDS
TCATTATAAGAAGATACAAT+TGG 0.458646 4.1:-12966521 None:intergenic
GGAAGAGACAGAAGTTGAGC+AGG 0.460748 4.1:+12968346 MS.gene27862:CDS
GAATCAGGCTTATGATTTGA+AGG 0.463037 4.1:+12968140 MS.gene27862:CDS
ACAATTGGTGCAACAGATAG+AGG 0.467988 4.1:-12966506 None:intergenic
TCTTTACTATCAAAGTCAGT+GGG 0.470875 4.1:-12966880 None:intergenic
TTATACTCCTCCAAGTACTC+AGG 0.473801 4.1:-12967988 None:intergenic
TGATACCAGCTAATGATTCA+AGG 0.474056 4.1:-12966664 None:intergenic
GAGCTTGCTGGCACTGCCAA+AGG 0.478600 4.1:+12966845 MS.gene27862:CDS
TCCTGAAGGTTTGCTGGAGA+AGG 0.484851 4.1:+12967156 MS.gene27862:CDS
GTTGTTGGAGATCAATCATC+TGG 0.485196 4.1:+12966629 MS.gene27862:CDS
AGCTTGAAGCACATGCTGAA+TGG 0.486139 4.1:-12967929 None:intergenic
TTATACTCCAAAACAAGCTC+TGG 0.492061 4.1:-12966761 None:intergenic
TATCAAAGTCAGTGGGGTGT+TGG 0.492352 4.1:-12966873 None:intergenic
TCCAATCCTGTCCATAATGT+TGG 0.494024 4.1:-12968406 None:intergenic
TTATCAGGTTGACCATGAAC+AGG 0.495576 4.1:-12966956 None:intergenic
GCGTCTCAACATTGCTAAAG+AGG 0.501157 4.1:+12966583 MS.gene27862:CDS
TGCCACTAAGGATTTCCTAA+TGG 0.506730 4.1:+12967651 MS.gene27862:CDS
TGTGTGGATATTTCATAAGC+TGG 0.507052 4.1:-12968113 None:intergenic
GTTTGTGTAAGGAATCGAAT+TGG 0.512590 4.1:+12967214 MS.gene27862:CDS
CTTATTGGAAAATTGTGCTC+CGG 0.520290 4.1:+12968175 MS.gene27862:CDS
GCCAGTGAGGATAAATTAGC+AGG 0.522213 4.1:-12967451 None:intergenic
AGATCAACCATAGTAAGATC+AGG 0.522883 4.1:-12966914 None:intergenic
AGTGTCCTTGAATCATTAGC+TGG 0.523168 4.1:+12966659 MS.gene27862:CDS
TCCAAAACAAGCTCTGGCTG+TGG 0.524796 4.1:-12966755 None:intergenic
GGTGCAACAGATAGAGGTTG+AGG 0.525331 4.1:-12966500 None:intergenic
TGTTGAAAGTAATGCCACTA+AGG 0.528407 4.1:+12967639 MS.gene27862:CDS
GCCAACATTATGGACAGGAT+TGG 0.530993 4.1:+12968405 MS.gene27862:CDS
AATGAACTGTTATCAGCAAA+TGG 0.537861 4.1:+12968279 MS.gene27862:CDS
ATTCAGCATATCTACTAATC+GGG 0.539015 4.1:-12967590 None:intergenic
ATCAGCTTGCCACGTGGACA+AGG 0.540401 4.1:+12966683 MS.gene27862:CDS
TCAGATTAAGGATATTATCA+TGG 0.543580 4.1:+12966988 MS.gene27862:CDS
GGAGGAAATCAAAGTTCTTG+AGG 0.547046 4.1:+12967672 MS.gene27862:CDS
AGCTTGCTGGCACTGCCAAA+GGG 0.547647 4.1:+12966846 MS.gene27862:CDS
TCTCCAACAACAACAATGGT+TGG 0.551269 4.1:-12966617 None:intergenic
TGTGAATGTTGATCTGCAAA+AGG 0.555960 4.1:+12968248 MS.gene27862:CDS
AGTGTCACACACTTCTGTCC+AGG 0.565122 4.1:+12967284 MS.gene27862:CDS
ACTTTGATAGTAAAGAAGAA+TGG 0.569006 4.1:+12966887 MS.gene27862:CDS
TCAAGTACTGTGAAGCTGAA+AGG 0.569995 4.1:+12968069 MS.gene27862:CDS
CACTAAGGATTTCCTAATGG+AGG 0.576778 4.1:+12967654 MS.gene27862:CDS
AGTGCCAGCAAGCTCCTCAG+TGG 0.578957 4.1:-12966837 None:intergenic
TTCTTTACTATCAAAGTCAG+TGG 0.579933 4.1:-12966881 None:intergenic
TATACTCCTCCAAGTACTCA+GGG 0.581284 4.1:-12967987 None:intergenic
CCGTGGCCAACATTATGGAC+AGG 0.581354 4.1:+12968400 MS.gene27862:CDS
CAATCACATCAATTAACCTC+CGG 0.585742 4.1:-12968194 None:intergenic
CGTCTCAACATTGCTAAAGA+GGG 0.585949 4.1:+12966584 MS.gene27862:CDS
AGGAGTCTCCTTCAATATCA+GGG 0.586223 4.1:+12968325 MS.gene27862:CDS
ATGTAACCCTGAGTACTTGG+AGG 0.589927 4.1:+12967981 MS.gene27862:CDS
CCTGTCCATAATGTTGGCCA+CGG 0.590976 4.1:-12968400 None:intergenic
GTGGACAAGGAATTTGTACA+AGG 0.592993 4.1:+12966696 MS.gene27862:CDS
GGACAGGATTGGAAATTATG+GGG 0.597404 4.1:+12968416 MS.gene27862:CDS
AAGGCTGATAAATCTCCTGA+AGG 0.599516 4.1:+12967142 MS.gene27862:CDS
CAAACAACTTTCAACTGATG+AGG 0.601066 4.1:+12966787 MS.gene27862:CDS
AACGCTTGCTGTCGTGACAA+AGG 0.611997 4.1:+12967123 MS.gene27862:CDS
ACAGGAACACGAGTTATACC+TGG 0.615115 4.1:-12966938 None:intergenic
TGGTGTTGAGAGGCTGCTTG+AGG 0.618613 4.1:+12968305 MS.gene27862:CDS
TGTGCTTCAAGCTGTTGAGA+TGG 0.619725 4.1:+12967939 MS.gene27862:CDS
TAAGCCTGATTCAACATGTG+TGG 0.620737 4.1:-12968129 None:intergenic
ATTGGAAAATTGTGCTCCGG+AGG 0.621567 4.1:+12968178 MS.gene27862:CDS
GGAGGAGTATAACAAGCTGA+TGG 0.621625 4.1:+12967999 MS.gene27862:CDS
ACAGCTGACGATGTTAACAT+TGG 0.623909 4.1:+12967181 MS.gene27862:CDS
AAGAGTCTTACGAAGATGCA+AGG 0.624326 4.1:+12967239 MS.gene27862:CDS
GATTTGCAGAAAAGCTGACA+TGG 0.626155 4.1:-12967479 None:intergenic
CTGTTATCAGCAAATGGTGG+TGG 0.626367 4.1:+12968285 MS.gene27862:CDS
CAAATGGTGGTGGTGTTGAG+AGG 0.637671 4.1:+12968295 MS.gene27862:CDS
ACTGTGAAGCTGAAAGGTGT+TGG 0.640769 4.1:+12968075 MS.gene27862:CDS
GAAAGAAAGCAAAGAGACCG+TGG 0.645229 4.1:+12968383 MS.gene27862:CDS
TTGATCTCCAACAACAACAA+TGG 0.648908 4.1:-12966621 None:intergenic
GTACAAATTCCTTGTCCACG+TGG 0.660130 4.1:-12966692 None:intergenic
TGACATGGCATCAGCCAGTG+AGG 0.671512 4.1:-12967464 None:intergenic
TGCTATAAATACAGCCACTG+AGG 0.694192 4.1:+12966823 MS.gene27862:CDS
CTTTACTATCAAAGTCAGTG+GGG 0.697240 4.1:-12966879 None:intergenic
GCTGGTATCAGCTTGCCACG+TGG 0.699523 4.1:+12966677 MS.gene27862:CDS
GTTGATCATCAACATCAACA+TGG 0.706155 4.1:-12966478 None:intergenic
TGGAAACAAGAGTTCCACAA+AGG 0.707464 4.1:+12966418 MS.gene27862:CDS
TTACTTCAGAGAAAAGTTAG+GGG 0.725881 4.1:+12967748 MS.gene27862:CDS
TGGACAAGGAATTTGTACAA+GGG 0.728664 4.1:+12966697 MS.gene27862:CDS
GAACTGTTATCAGCAAATGG+TGG 0.747464 4.1:+12968282 MS.gene27862:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ACACTTTCAACAAAATTAAT+TGG - chr4.1:12967787-12967806 None:intergenic 20.0%
!! CTGAAAAAAATTCTTCTTAT+AGG + chr4.1:12967523-12967542 MS.gene27862:CDS 20.0%
!! TAACATTTATGATCAGATTA+AGG + chr4.1:12966976-12966995 MS.gene27862:CDS 20.0%
!! TCATTATAAGAAGATACAAT+TGG - chr4.1:12966524-12966543 None:intergenic 20.0%
!!! TTAATTTTGTTGAAAGTGTA+TGG + chr4.1:12967788-12967807 MS.gene27862:CDS 20.0%
! AATTCAGCATATCTACTAAT+CGG - chr4.1:12967594-12967613 None:intergenic 25.0%
! ATCTGATCATAAATGTTATC+AGG - chr4.1:12966974-12966993 None:intergenic 25.0%
! TAAGAAGATTAATGAGAAAC+TGG + chr4.1:12967399-12967418 MS.gene27862:CDS 25.0%
! TCAGATTAAGGATATTATCA+TGG + chr4.1:12966988-12967007 MS.gene27862:CDS 25.0%
! TTAATCTTCTTAATGATCTC+TGG - chr4.1:12967391-12967410 None:intergenic 25.0%
! TTCAAAATAATACTCTCTTC+AGG - chr4.1:12967025-12967044 None:intergenic 25.0%
!! ACTTTGATAGTAAAGAAGAA+TGG + chr4.1:12966887-12966906 MS.gene27862:CDS 25.0%
!! TCTTTTTCTTCTTAGCAATA+AGG - chr4.1:12967906-12967925 None:intergenic 25.0%
AACAATAGATTTGTCTATCC+TGG - chr4.1:12967305-12967324 None:intergenic 30.0%
AATGAACTGTTATCAGCAAA+TGG + chr4.1:12968279-12968298 MS.gene27862:CDS 30.0%
AGGATAGACAAATCTATTGT+TGG + chr4.1:12967304-12967323 MS.gene27862:CDS 30.0%
ATTCAGCATATCTACTAATC+GGG - chr4.1:12967593-12967612 None:intergenic 30.0%
ATTCGGAAGACCAATAAATT+TGG - chr4.1:12967701-12967720 None:intergenic 30.0%
CTTACTTCAGAGAAAAGTTA+GGG + chr4.1:12967747-12967766 MS.gene27862:CDS 30.0%
TCTTTACTATCAAAGTCAGT+GGG - chr4.1:12966883-12966902 None:intergenic 30.0%
TTACTTCAGAGAAAAGTTAG+GGG + chr4.1:12967748-12967767 MS.gene27862:CDS 30.0%
TTCTAACTATTATCAGCTTC+AGG + chr4.1:12967855-12967874 MS.gene27862:CDS 30.0%
TTCTTTACTATCAAAGTCAG+TGG - chr4.1:12966884-12966903 None:intergenic 30.0%
TTGATGCTCTTGAAAATCTA+AGG + chr4.1:12966561-12966580 MS.gene27862:CDS 30.0%
! TATTGTTGGAATTCCTGTTT+TGG + chr4.1:12967318-12967337 MS.gene27862:CDS 30.0%
! TCACAAACTGTTGATAAAAC+TGG + chr4.1:12967094-12967113 MS.gene27862:CDS 30.0%
! TGAAAGTGTATGGAATTATC+TGG + chr4.1:12967798-12967817 MS.gene27862:CDS 30.0%
AGATCAACCATAGTAAGATC+AGG - chr4.1:12966917-12966936 None:intergenic 35.0%
ATATCCACACATGTTGAATC+AGG + chr4.1:12968125-12968144 MS.gene27862:CDS 35.0%
ATGGACAGGATTGGAAATTA+TGG + chr4.1:12968414-12968433 MS.gene27862:CDS 35.0%
CAAACAACTTTCAACTGATG+AGG + chr4.1:12966787-12966806 MS.gene27862:CDS 35.0%
CAATCACATCAATTAACCTC+CGG - chr4.1:12968197-12968216 None:intergenic 35.0%
CCTTACTTCAGAGAAAAGTT+AGG + chr4.1:12967746-12967765 MS.gene27862:CDS 35.0%
CTTATTGGAAAATTGTGCTC+CGG + chr4.1:12968175-12968194 MS.gene27862:CDS 35.0%
CTTGAGGAAGCCAAATTTAT+TGG + chr4.1:12967688-12967707 MS.gene27862:CDS 35.0%
CTTTACTATCAAAGTCAGTG+GGG - chr4.1:12966882-12966901 None:intergenic 35.0%
GAATCAGGCTTATGATTTGA+AGG + chr4.1:12968140-12968159 MS.gene27862:CDS 35.0%
GTCTTGGTTATGTTTGTGTA+AGG + chr4.1:12967203-12967222 MS.gene27862:CDS 35.0%
GTTGATCATCAACATCAACA+TGG - chr4.1:12966481-12966500 None:intergenic 35.0%
GTTTGTGTAAGGAATCGAAT+TGG + chr4.1:12967214-12967233 MS.gene27862:CDS 35.0%
TGATACCAGCTAATGATTCA+AGG - chr4.1:12966667-12966686 None:intergenic 35.0%
TGGACAAGGAATTTGTACAA+GGG + chr4.1:12966697-12966716 MS.gene27862:CDS 35.0%
TGGACAGGATTGGAAATTAT+GGG + chr4.1:12968415-12968434 MS.gene27862:CDS 35.0%
TGTGAATGTTGATCTGCAAA+AGG + chr4.1:12968248-12968267 MS.gene27862:CDS 35.0%
TGTGTGGATATTTCATAAGC+TGG - chr4.1:12968116-12968135 None:intergenic 35.0%
TGTTGAAAGTAATGCCACTA+AGG + chr4.1:12967639-12967658 MS.gene27862:CDS 35.0%
TTACTACTTGTGAGTCGATA+AGG + chr4.1:12967068-12967087 MS.gene27862:CDS 35.0%
TTATACTCCAAAACAAGCTC+TGG - chr4.1:12966764-12966783 None:intergenic 35.0%
TTGATCTCCAACAACAACAA+TGG - chr4.1:12966624-12966643 None:intergenic 35.0%
TTTCCAATAAGCTGTCAATC+TGG - chr4.1:12968166-12968185 None:intergenic 35.0%
! ATCAAGTTTGTTTGCCTTTG+TGG - chr4.1:12966435-12966454 None:intergenic 35.0%
! GAACTTTGATTTCCTCCATT+AGG - chr4.1:12967669-12967688 None:intergenic 35.0%
!! AATTTGTACAAGGGTACCTT+TGG + chr4.1:12966706-12966725 MS.gene27862:CDS 35.0%
!!! CCTAACTTTTCTCTGAAGTA+AGG - chr4.1:12967749-12967768 None:intergenic 35.0%
!!! GAGCTTGTTTTGGAGTATAA+TGG + chr4.1:12966764-12966783 MS.gene27862:CDS 35.0%
AAGAGTCTTACGAAGATGCA+AGG + chr4.1:12967239-12967258 MS.gene27862:CDS 40.0%
AAGGCTGATAAATCTCCTGA+AGG + chr4.1:12967142-12967161 MS.gene27862:CDS 40.0%
AAGGTCAGAAAAGCAGTTCT+TGG - chr4.1:12967730-12967749 None:intergenic 40.0%
ACAATTGGTGCAACAGATAG+AGG - chr4.1:12966509-12966528 None:intergenic 40.0%
ACAGCTGACGATGTTAACAT+TGG + chr4.1:12967181-12967200 MS.gene27862:CDS 40.0%
AGGAGTCTCCTTCAATATCA+GGG + chr4.1:12968325-12968344 MS.gene27862:CDS 40.0%
AGTGTCCTTGAATCATTAGC+TGG + chr4.1:12966659-12966678 MS.gene27862:CDS 40.0%
ATAACTCGTGTTCCTGTTCA+TGG + chr4.1:12966944-12966963 MS.gene27862:CDS 40.0%
CACTAAGGATTTCCTAATGG+AGG + chr4.1:12967654-12967673 MS.gene27862:CDS 40.0%
CGTCTCAACATTGCTAAAGA+GGG + chr4.1:12966584-12966603 MS.gene27862:CDS 40.0%
CTCCATTAGGAAATCCTTAG+TGG - chr4.1:12967656-12967675 None:intergenic 40.0%
CTTACTATGGTTGATCTTCC+AGG + chr4.1:12966920-12966939 MS.gene27862:CDS 40.0%
GAACTGTTATCAGCAAATGG+TGG + chr4.1:12968282-12968301 MS.gene27862:CDS 40.0%
GATTTGCAGAAAAGCTGACA+TGG - chr4.1:12967482-12967501 None:intergenic 40.0%
GCAGTTCTTGGCATGAAATT+CGG - chr4.1:12967718-12967737 None:intergenic 40.0%
GGACAGGATTGGAAATTATG+GGG + chr4.1:12968416-12968435 MS.gene27862:CDS 40.0%
GGAGGAAATCAAAGTTCTTG+AGG + chr4.1:12967672-12967691 MS.gene27862:CDS 40.0%
GTGGACAAGGAATTTGTACA+AGG + chr4.1:12966696-12966715 MS.gene27862:CDS 40.0%
GTTGTTGGAGATCAATCATC+TGG + chr4.1:12966629-12966648 MS.gene27862:CDS 40.0%
TAAATCTCCTGAAGGTTTGC+TGG + chr4.1:12967150-12967169 MS.gene27862:CDS 40.0%
TAAGCCTGATTCAACATGTG+TGG - chr4.1:12968132-12968151 None:intergenic 40.0%
TATACTCCTCCAAGTACTCA+GGG - chr4.1:12967990-12968009 None:intergenic 40.0%
TCCAATCCTGTCCATAATGT+TGG - chr4.1:12968409-12968428 None:intergenic 40.0%
TCTCCAACAACAACAATGGT+TGG - chr4.1:12966620-12966639 None:intergenic 40.0%
TGCCACTAAGGATTTCCTAA+TGG + chr4.1:12967651-12967670 MS.gene27862:CDS 40.0%
TGCTATAAATACAGCCACTG+AGG + chr4.1:12966823-12966842 MS.gene27862:CDS 40.0%
TGGAAACAAGAGTTCCACAA+AGG + chr4.1:12966418-12966437 MS.gene27862:CDS 40.0%
TGGAGTTCCTGATCTTACTA+TGG + chr4.1:12966907-12966926 MS.gene27862:CDS 40.0%
TGTCTCTTCCCTGATATTGA+AGG - chr4.1:12968336-12968355 None:intergenic 40.0%
TTATACTCCTCCAAGTACTC+AGG - chr4.1:12967991-12968010 None:intergenic 40.0%
! ACAAGCTTTTGAGCCAAAAC+AGG - chr4.1:12967334-12967353 None:intergenic 40.0%
! CTTCCAACCATTGTTGTTGT+TGG + chr4.1:12966614-12966633 MS.gene27862:CDS 40.0%
! GACGATGTTAACATTGGTCT+TGG + chr4.1:12967187-12967206 MS.gene27862:CDS 40.0%
! TCAAGTACTGTGAAGCTGAA+AGG + chr4.1:12968069-12968088 MS.gene27862:CDS 40.0%
! TTTTGCAGCCTCATAACCAA+AGG - chr4.1:12966725-12966744 None:intergenic 40.0%
!! TTATCAGGTTGACCATGAAC+AGG - chr4.1:12966959-12966978 None:intergenic 40.0%
!!! TTTTCAAGAGCATCAAGCAC+TGG - chr4.1:12966557-12966576 None:intergenic 40.0%
ACAGGAACACGAGTTATACC+TGG - chr4.1:12966941-12966960 None:intergenic 45.0%
AGCTTGAAGCACATGCTGAA+TGG - chr4.1:12967932-12967951 None:intergenic 45.0%
AGGCCAGATTGACAGCTTAT+TGG + chr4.1:12968160-12968179 MS.gene27862:CDS 45.0%
ATGTAACCCTGAGTACTTGG+AGG + chr4.1:12967981-12968000 MS.gene27862:CDS 45.0%
ATTGGAAAATTGTGCTCCGG+AGG + chr4.1:12968178-12968197 MS.gene27862:CDS 45.0%
CACATGTAACCCTGAGTACT+TGG + chr4.1:12967978-12967997 MS.gene27862:CDS 45.0%
CTGTTATCAGCAAATGGTGG+TGG + chr4.1:12968285-12968304 MS.gene27862:CDS 45.0%
GAAAGAAAGCAAAGAGACCG+TGG + chr4.1:12968383-12968402 MS.gene27862:CDS 45.0%
GAGGAGTCTCCTTCAATATC+AGG + chr4.1:12968324-12968343 MS.gene27862:CDS 45.0%
GCCAACATTATGGACAGGAT+TGG + chr4.1:12968405-12968424 MS.gene27862:CDS 45.0%
GCCAGTGAGGATAAATTAGC+AGG - chr4.1:12967454-12967473 None:intergenic 45.0%
GCCTGCTAATTTATCCTCAC+TGG + chr4.1:12967450-12967469 MS.gene27862:CDS 45.0%
GCGTCTCAACATTGCTAAAG+AGG + chr4.1:12966583-12966602 MS.gene27862:CDS 45.0%
GTACAAATTCCTTGTCCACG+TGG - chr4.1:12966695-12966714 None:intergenic 45.0%
TATCAAAGTCAGTGGGGTGT+TGG - chr4.1:12966876-12966895 None:intergenic 45.0%
TGTGCTTCAAGCTGTTGAGA+TGG + chr4.1:12967939-12967958 MS.gene27862:CDS 45.0%
! ACTGTGAAGCTGAAAGGTGT+TGG + chr4.1:12968075-12968094 MS.gene27862:CDS 45.0%
! GGAGGAGTATAACAAGCTGA+TGG + chr4.1:12967999-12968018 MS.gene27862:CDS 45.0%
!! CAAGGGTACCTTTGGTTATG+AGG + chr4.1:12966714-12966733 MS.gene27862:CDS 45.0%
ACCTTCTCCAGCAAACCTTC+AGG - chr4.1:12967160-12967179 None:intergenic 50.0%
AGAGACCGTGGCCAACATTA+TGG + chr4.1:12968395-12968414 MS.gene27862:CDS 50.0%
AGTGTCACACACTTCTGTCC+AGG + chr4.1:12967284-12967303 MS.gene27862:CDS 50.0%
CCTGTCCATAATGTTGGCCA+CGG - chr4.1:12968403-12968422 None:intergenic 50.0%
GCTCTGGCTGTGGAAGTAAA+TGG - chr4.1:12966748-12966767 None:intergenic 50.0%
GGAAGAGACAGAAGTTGAGC+AGG + chr4.1:12968346-12968365 MS.gene27862:CDS 50.0%
GGTGCAACAGATAGAGGTTG+AGG - chr4.1:12966503-12966522 None:intergenic 50.0%
! GGCTACTAGTTTGCATGAGC+TGG + chr4.1:12967420-12967439 MS.gene27862:CDS 50.0%
! TCCAAAACAAGCTCTGGCTG+TGG - chr4.1:12966758-12966777 None:intergenic 50.0%
!! AACGCTTGCTGTCGTGACAA+AGG + chr4.1:12967123-12967142 MS.gene27862:CDS 50.0%
!! CAAATGGTGGTGGTGTTGAG+AGG + chr4.1:12968295-12968314 MS.gene27862:CDS 50.0%
!! TCCACAGCCAGAGCTTGTTT+TGG + chr4.1:12966754-12966773 MS.gene27862:CDS 50.0%
!! TCCTGAAGGTTTGCTGGAGA+AGG + chr4.1:12967156-12967175 MS.gene27862:CDS 50.0%
!! TGGGGTGTTGGAAATCCCTT+TGG - chr4.1:12966864-12966883 None:intergenic 50.0%
ATCAGCTTGCCACGTGGACA+AGG + chr4.1:12966683-12966702 MS.gene27862:CDS 55.0%
CCGTGGCCAACATTATGGAC+AGG + chr4.1:12968400-12968419 MS.gene27862:CDS 55.0%
TGACATGGCATCAGCCAGTG+AGG - chr4.1:12967467-12967486 None:intergenic 55.0%
! TGGTGTTGAGAGGCTGCTTG+AGG + chr4.1:12968305-12968324 MS.gene27862:CDS 55.0%
!! AGCTTGCTGGCACTGCCAAA+GGG + chr4.1:12966846-12966865 MS.gene27862:CDS 55.0%
ACAGCCACTGAGGAGCTTGC+TGG + chr4.1:12966833-12966852 MS.gene27862:CDS 60.0%
AGTGCCAGCAAGCTCCTCAG+TGG - chr4.1:12966840-12966859 None:intergenic 60.0%
GCTGGTATCAGCTTGCCACG+TGG + chr4.1:12966677-12966696 MS.gene27862:CDS 60.0%
!! GAGCTTGCTGGCACTGCCAA+AGG + chr4.1:12966845-12966864 MS.gene27862:CDS 60.0%


Chromosome Type Strat End Strand Name
chr4.1 gene 12966410 12968446 12966410 ID=MS.gene27862
chr4.1 mRNA 12966410 12968446 12966410 ID=MS.gene27862.t1;Parent=MS.gene27862
chr4.1 exon 12966410 12968446 12966410 ID=MS.gene27862.t1.exon1;Parent=MS.gene27862.t1
chr4.1 CDS 12966410 12968446 12966410 ID=cds.MS.gene27862.t1;Parent=MS.gene27862.t1
Gene Sequence

>MS.gene27862

ATGACAATTGGAAACAAGAGTTCCACAAAGGCAAACAAACTTGATGAATCATCATCACTTGCATTAGTCCATGTTGATGTTGATGATCAACCTCAACCTCTATCTGTTGCACCAATTGTATCTTCTTATAATGAGAAGATACGTCCAGTGCTTGATGCTCTTGAAAATCTAAGGCGTCTCAACATTGCTAAAGAGGGAATTCAGCTTCCAACCATTGTTGTTGTTGGAGATCAATCATCTGGAAAGTCTAGTGTCCTTGAATCATTAGCTGGTATCAGCTTGCCACGTGGACAAGGAATTTGTACAAGGGTACCTTTGGTTATGAGGCTGCAAAACCATTTACTTCCACAGCCAGAGCTTGTTTTGGAGTATAATGGCAAACAACTTTCAACTGATGAGGAAAATGTATCTGATGCTATAAATACAGCCACTGAGGAGCTTGCTGGCACTGCCAAAGGGATTTCCAACACCCCACTGACTTTGATAGTAAAGAAGAATGGAGTTCCTGATCTTACTATGGTTGATCTTCCAGGTATAACTCGTGTTCCTGTTCATGGTCAACCTGATAACATTTATGATCAGATTAAGGATATTATCATGGAGTATATAACTCCTGAAGAGAGTATTATTTTGAATGTGCTCTCTGCTTCTGTTGATTTTACTACTTGTGAGTCGATAAGGATGTCACAAACTGTTGATAAAACTGGTTTGAGAACGCTTGCTGTCGTGACAAAGGCTGATAAATCTCCTGAAGGTTTGCTGGAGAAGGTTACAGCTGACGATGTTAACATTGGTCTTGGTTATGTTTGTGTAAGGAATCGAATTGGTGAAGAGTCTTACGAAGATGCAAGGAATGAAGAAGAGAAGCTGTTCGAGTGTCACACACTTCTGTCCAGGATAGACAAATCTATTGTTGGAATTCCTGTTTTGGCTCAAAAGCTTGTTCACGTTCAAGCGATGATTATATCTAAAACTTTGCCAGAGATCATTAAGAAGATTAATGAGAAACTGGCTACTAGTTTGCATGAGCTGGAAACTTTGCCTGCTAATTTATCCTCACTGGCTGATGCCATGTCAGCTTTTCTGCAAATCATTTCTTTGTCAAGAGACTCTCTGAAAAAAATTCTTCTTATAGGAGAGTTTGAAGAGTATTCTGATGAAAAGAAAATGCATTGCACAGCCCGATTAGTAGATATGCTGAATTCATATGCAAGTGAGCTTCAAAACTGTGTTGAAAGTAATGCCACTAAGGATTTCCTAATGGAGGAAATCAAAGTTCTTGAGGAAGCCAAATTTATTGGTCTTCCGAATTTCATGCCAAGAACTGCTTTTCTGACCTTACTTCAGAGAAAAGTTAGGGGCATTTCTTATATGCCAATTAATTTTGTTGAAAGTGTATGGAATTATCTGGAAACTGTTGTTGTTTCAGTTTTGAATCGTCATTCTTCTAACTATTATCAGCTTCAGGTTTCAATTCGTAGAGCTGCTGAGCACCTTATTGCTAAGAAGAAAAAGAATTCCATTCAGCATGTGCTTCAAGCTGTTGAGATGGAGAAGCTTACAGACTACACATGTAACCCTGAGTACTTGGAGGAGTATAACAAGCTGATGGCTCATCAAGAAGTGTTTTTGAAAGAAGTTTTGAATGTTAAACGAGCTTCAAGTACTGTGAAGCTGAAAGGTGTTGGTGACGTTGATGTTGTCCAGCTTATGAAATATCCACACATGTTGAATCAGGCTTATGATTTGAAGGCCAGATTGACAGCTTATTGGAAAATTGTGCTCCGGAGGTTAATTGATGTGATTGCTTTGCATTTGATGTTAAGCATCAATGAACTTGTGAATGTTGATCTGCAAAAGGAGATCTGTAATGAACTGTTATCAGCAAATGGTGGTGGTGTTGAGAGGCTGCTTGAGGAGTCTCCTTCAATATCAGGGAAGAGACAGAAGTTGAGCAGGAGTGTGACAGTTTTGAAAGAAAGCAAAGAGACCGTGGCCAACATTATGGACAGGATTGGAAATTATGGGGATAACTAG
Protein sequence

>MS.gene27862.t1

MTIGNKSSTKANKLDESSSLALVHVDVDDQPQPLSVAPIVSSYNEKIRPVLDALENLRRLNIAKEGIQLPTIVVVGDQSSGKSSVLESLAGISLPRGQGICTRVPLVMRLQNHLLPQPELVLEYNGKQLSTDEENVSDAINTATEELAGTAKGISNTPLTLIVKKNGVPDLTMVDLPGITRVPVHGQPDNIYDQIKDIIMEYITPEESIILNVLSASVDFTTCESIRMSQTVDKTGLRTLAVVTKADKSPEGLLEKVTADDVNIGLGYVCVRNRIGEESYEDARNEEEKLFECHTLLSRIDKSIVGIPVLAQKLVHVQAMIISKTLPEIIKKINEKLATSLHELETLPANLSSLADAMSAFLQIISLSRDSLKKILLIGEFEEYSDEKKMHCTARLVDMLNSYASELQNCVESNATKDFLMEEIKVLEEAKFIGLPNFMPRTAFLTLLQRKVRGISYMPINFVESVWNYLETVVVSVLNRHSSNYYQLQVSIRRAAEHLIAKKKKNSIQHVLQAVEMEKLTDYTCNPEYLEEYNKLMAHQEVFLKEVLNVKRASSTVKLKGVGDVDVVQLMKYPHMLNQAYDLKARLTAYWKIVLRRLIDVIALHLMLSINELVNVDLQKEICNELLSANGGGVERLLEESPSISGKRQKLSRSVTVLKESKETVANIMDRIGNYGDN