Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene29144.t1 | XP_003613091.1 | 94.9 | 177 | 3 | 1 | 1 | 171 | 1 | 177 | 8.20E-84 | 319.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene29144.t1 | G7JXR4 | 94.9 | 177 | 3 | 1 | 1 | 171 | 1 | 177 | 5.9e-84 | 319.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene053074 | MS.gene29144 | 0.80813 | 3.60E-50 | -1.69E-46 |
| MS.gene054184 | MS.gene29144 | 0.809218 | 2.11E-50 | -1.69E-46 |
| MS.gene054185 | MS.gene29144 | 0.810505 | 1.11E-50 | -1.69E-46 |
| MS.gene055133 | MS.gene29144 | 0.803999 | 2.67E-49 | -1.69E-46 |
| MS.gene057754 | MS.gene29144 | 0.814794 | 1.28E-51 | -1.69E-46 |
| MS.gene059124 | MS.gene29144 | 0.846012 | 2.84E-59 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene29144.t1 | MTR_5g032580 | 94.915 | 177 | 3 | 1 | 1 | 171 | 1 | 177 | 3.43e-119 | 334 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene29144.t1 | AT1G26290 | 43.119 | 109 | 58 | 2 | 6 | 110 | 1 | 109 | 2.07e-16 | 72.0 |
Find 32 sgRNAs with CRISPR-Local
Find 39 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGATTTAGAAGAGGAGATTA+AGG | 0.230236 | 5.3:+21687489 | MS.gene29144:CDS |
| TAACAAAGAAGCAAATAAAA+AGG | 0.238986 | 5.3:+21687663 | MS.gene29144:CDS |
| CACTTTCTGAGGTGATTATA+AGG | 0.320343 | 5.3:+21687599 | MS.gene29144:CDS |
| TTCAACTCCTCTGCTGAAAT+AGG | 0.331112 | 5.3:-21687433 | None:intergenic |
| AAGAAGTGAAGAAGATTGAT+TGG | 0.347744 | 5.3:+21687728 | MS.gene29144:CDS |
| TATGATCCTTTCACAGGAAC+AGG | 0.362440 | 5.3:-21687778 | None:intergenic |
| GGATAAGAAGCTACTTCAAT+TGG | 0.436350 | 5.3:+21687855 | MS.gene29144:CDS |
| TTGATTGGGAGAAGAGTTTG+AGG | 0.445900 | 5.3:+21687743 | MS.gene29144:CDS |
| AGGAACTAAAATTGAAATCA+AGG | 0.471855 | 5.3:+21687636 | MS.gene29144:CDS |
| TTAGAAGAGGAGATTAAGGA+AGG | 0.483543 | 5.3:+21687493 | MS.gene29144:CDS |
| GGTGATTATAAGGATGAGAA+TGG | 0.489148 | 5.3:+21687609 | MS.gene29144:CDS |
| AAAGGATAAAATAATGATGA+GGG | 0.497614 | 5.3:+21687816 | MS.gene29144:CDS |
| TGATTGGGAGAAGAGTTTGA+GGG | 0.505362 | 5.3:+21687744 | MS.gene29144:CDS |
| GCTACTTCAATTGGTGTGGA+AGG | 0.513216 | 5.3:+21687864 | MS.gene29144:CDS |
| ATTATAAGGATGAGAATGGA+AGG | 0.528677 | 5.3:+21687613 | MS.gene29144:CDS |
| AGAAGTGAAGAAGATTGATT+GGG | 0.529976 | 5.3:+21687729 | MS.gene29144:CDS |
| TCAAGCCCTGTTCCTGTGAA+AGG | 0.530825 | 5.3:+21687772 | MS.gene29144:CDS |
| ATATGTTAGATCAAAGCAAA+AGG | 0.536682 | 5.3:+21687798 | MS.gene29144:CDS |
| CTAACATATGATCCTTTCAC+AGG | 0.563019 | 5.3:-21687784 | None:intergenic |
| AAAAGGATAAAATAATGATG+AGG | 0.564917 | 5.3:+21687815 | MS.gene29144:CDS |
| CAAAATTAGAGCACTTTCTG+AGG | 0.566466 | 5.3:+21687588 | MS.gene29144:CDS |
| GATTGAAAGAGATTTAGAAG+AGG | 0.567737 | 5.3:+21687480 | MS.gene29144:CDS |
| TGGAATAACATGGATCGTCA+TGG | 0.568789 | 5.3:+21687280 | None:intergenic |
| GAGGGGAAAGAATGCAAGTG+AGG | 0.574468 | 5.3:+21687834 | MS.gene29144:CDS |
| TGAGGTTCCTATTTCAGCAG+AGG | 0.574672 | 5.3:+21687426 | MS.gene29144:CDS |
| AGAAGCTACTTCAATTGGTG+TGG | 0.577937 | 5.3:+21687860 | MS.gene29144:CDS |
| TGGGAGAAGAGTTTGAGGGC+AGG | 0.600710 | 5.3:+21687748 | MS.gene29144:CDS |
| ATAAGGATGAGAATGGAAGG+AGG | 0.636221 | 5.3:+21687616 | MS.gene29144:CDS |
| ATGATCCTTTCACAGGAACA+GGG | 0.639503 | 5.3:-21687777 | None:intergenic |
| CGTCATGGAATGTACACCAA+AGG | 0.643271 | 5.3:+21687295 | MS.gene29144:CDS |
| AAGGATAAAATAATGATGAG+GGG | 0.646560 | 5.3:+21687817 | MS.gene29144:CDS |
| AACTAAAATTGAAATCAAGG+AGG | 0.721284 | 5.3:+21687639 | MS.gene29144:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAAGGATAAAATAATGATG+AGG | + | chr5.3:21687815-21687834 | MS.gene29144:CDS | 20.0% |
| !! | AAAGGATAAAATAATGATGA+GGG | + | chr5.3:21687816-21687835 | MS.gene29144:CDS | 20.0% |
| !! | AACAAAGAAGCAAATAAAAA+GGG | + | chr5.3:21687664-21687683 | MS.gene29144:CDS | 20.0% |
| !! | TAACAAAGAAGCAAATAAAA+AGG | + | chr5.3:21687663-21687682 | MS.gene29144:CDS | 20.0% |
| !!! | ACTTCTTTCAAACATTTTTT+GGG | - | chr5.3:21687715-21687734 | None:intergenic | 20.0% |
| ! | AACTAAAATTGAAATCAAGG+AGG | + | chr5.3:21687639-21687658 | MS.gene29144:CDS | 25.0% |
| ! | AAGGATAAAATAATGATGAG+GGG | + | chr5.3:21687817-21687836 | MS.gene29144:CDS | 25.0% |
| ! | AGGAACTAAAATTGAAATCA+AGG | + | chr5.3:21687636-21687655 | MS.gene29144:CDS | 25.0% |
| ! | ATATGTTAGATCAAAGCAAA+AGG | + | chr5.3:21687798-21687817 | MS.gene29144:CDS | 25.0% |
| ! | GTCAAATAAAAATGTTCTTG+AGG | + | chr5.3:21687408-21687427 | MS.gene29144:CDS | 25.0% |
| !! | AACGAATTTAATTTCACCTT+TGG | - | chr5.3:21687314-21687333 | None:intergenic | 25.0% |
| !!! | CACTTCTTTCAAACATTTTT+TGG | - | chr5.3:21687716-21687735 | None:intergenic | 25.0% |
| !!! | CATTTTTTGGGTCTAAAATT+TGG | - | chr5.3:21687703-21687722 | None:intergenic | 25.0% |
| AAGAAGTGAAGAAGATTGAT+TGG | + | chr5.3:21687728-21687747 | MS.gene29144:CDS | 30.0% | |
| AGAAGTGAAGAAGATTGATT+GGG | + | chr5.3:21687729-21687748 | MS.gene29144:CDS | 30.0% | |
| AGATTTAGAAGAGGAGATTA+AGG | + | chr5.3:21687489-21687508 | MS.gene29144:CDS | 30.0% | |
| ATTATAAGGATGAGAATGGA+AGG | + | chr5.3:21687613-21687632 | MS.gene29144:CDS | 30.0% | |
| GATTGAAAGAGATTTAGAAG+AGG | + | chr5.3:21687480-21687499 | MS.gene29144:CDS | 30.0% | |
| CTAACATATGATCCTTTCAC+AGG | - | chr5.3:21687787-21687806 | None:intergenic | 35.0% | |
| GGATAAGAAGCTACTTCAAT+TGG | + | chr5.3:21687855-21687874 | MS.gene29144:CDS | 35.0% | |
| GGTGATTATAAGGATGAGAA+TGG | + | chr5.3:21687609-21687628 | MS.gene29144:CDS | 35.0% | |
| TTAGAAGAGGAGATTAAGGA+AGG | + | chr5.3:21687493-21687512 | MS.gene29144:CDS | 35.0% | |
| ! | CAAAATTAGAGCACTTTCTG+AGG | + | chr5.3:21687588-21687607 | MS.gene29144:CDS | 35.0% |
| !! | CACTTTCTGAGGTGATTATA+AGG | + | chr5.3:21687599-21687618 | MS.gene29144:CDS | 35.0% |
| AGAAGCTACTTCAATTGGTG+TGG | + | chr5.3:21687860-21687879 | MS.gene29144:CDS | 40.0% | |
| ATAAGGATGAGAATGGAAGG+AGG | + | chr5.3:21687616-21687635 | MS.gene29144:CDS | 40.0% | |
| ATGATCCTTTCACAGGAACA+GGG | - | chr5.3:21687780-21687799 | None:intergenic | 40.0% | |
| TATGATCCTTTCACAGGAAC+AGG | - | chr5.3:21687781-21687800 | None:intergenic | 40.0% | |
| TGATTGGGAGAAGAGTTTGA+GGG | + | chr5.3:21687744-21687763 | MS.gene29144:CDS | 40.0% | |
| TTCAACTCCTCTGCTGAAAT+AGG | - | chr5.3:21687436-21687455 | None:intergenic | 40.0% | |
| TTGATTGGGAGAAGAGTTTG+AGG | + | chr5.3:21687743-21687762 | MS.gene29144:CDS | 40.0% | |
| !! | TCGAATTTTTCAGCAGAGGA+AGG | + | chr5.3:21687543-21687562 | MS.gene29144:CDS | 40.0% |
| !! | TGCTTCGAATTTTTCAGCAG+AGG | + | chr5.3:21687539-21687558 | MS.gene29144:CDS | 40.0% |
| CGTCATGGAATGTACACCAA+AGG | + | chr5.3:21687295-21687314 | MS.gene29144:CDS | 45.0% | |
| GCTACTTCAATTGGTGTGGA+AGG | + | chr5.3:21687864-21687883 | MS.gene29144:CDS | 45.0% | |
| TGAGGTTCCTATTTCAGCAG+AGG | + | chr5.3:21687426-21687445 | MS.gene29144:CDS | 45.0% | |
| GAGGGGAAAGAATGCAAGTG+AGG | + | chr5.3:21687834-21687853 | MS.gene29144:CDS | 50.0% | |
| TCAAGCCCTGTTCCTGTGAA+AGG | + | chr5.3:21687772-21687791 | MS.gene29144:CDS | 50.0% | |
| TGGGAGAAGAGTTTGAGGGC+AGG | + | chr5.3:21687748-21687767 | MS.gene29144:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.3 | gene | 21687289 | 21687891 | 21687289 | ID=MS.gene29144 |
| chr5.3 | mRNA | 21687289 | 21687891 | 21687289 | ID=MS.gene29144.t1;Parent=MS.gene29144 |
| chr5.3 | exon | 21687289 | 21687316 | 21687289 | ID=MS.gene29144.t1.exon1;Parent=MS.gene29144.t1 |
| chr5.3 | CDS | 21687289 | 21687316 | 21687289 | ID=cds.MS.gene29144.t1;Parent=MS.gene29144.t1 |
| chr5.3 | exon | 21687404 | 21687891 | 21687404 | ID=MS.gene29144.t1.exon2;Parent=MS.gene29144.t1 |
| chr5.3 | CDS | 21687404 | 21687891 | 21687404 | ID=cds.MS.gene29144.t1;Parent=MS.gene29144.t1 |
| Gene Sequence |
| Protein sequence |
