Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene29253.t1 | XP_013466900.1 | 89.1 | 46 | 5 | 0 | 1 | 46 | 109 | 154 | 7.30E-14 | 85.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene29253.t1 | Q9FMX7 | 72.7 | 44 | 12 | 0 | 3 | 46 | 106 | 149 | 3.8e-11 | 68.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene29253.t1 | A0A072VH64 | 89.1 | 46 | 5 | 0 | 1 | 46 | 109 | 154 | 5.2e-14 | 85.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049701 | MS.gene29253 | 0.813702 | 2.24E-51 | -1.69E-46 |
| MS.gene049853 | MS.gene29253 | 0.827783 | 1.29E-54 | -1.69E-46 |
| MS.gene051710 | MS.gene29253 | 0.805679 | 1.19E-49 | -1.69E-46 |
| MS.gene054484 | MS.gene29253 | 0.813937 | 1.99E-51 | -1.69E-46 |
| MS.gene055058 | MS.gene29253 | 0.80603 | 1.00E-49 | -1.69E-46 |
| MS.gene058515 | MS.gene29253 | 0.848566 | 5.65E-60 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene29253.t1 | MTR_1g037830 | 89.130 | 46 | 5 | 0 | 1 | 46 | 109 | 154 | 5.72e-23 | 86.3 |
| MS.gene29253.t1 | MTR_1g038530 | 89.130 | 46 | 5 | 0 | 1 | 46 | 109 | 154 | 5.90e-23 | 86.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene29253.t1 | AT5G23230 | 72.727 | 44 | 12 | 0 | 3 | 46 | 106 | 149 | 3.99e-16 | 68.6 |
| MS.gene29253.t1 | AT5G23220 | 65.909 | 44 | 15 | 0 | 3 | 46 | 106 | 149 | 9.83e-14 | 62.4 |
Find 12 sgRNAs with CRISPR-Local
Find 12 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CGCATTTAGGAACACAGGTT+TGG | 0.359514 | 1.1:-22948776 | MS.gene29253:CDS |
| AGAGTACATACAACGCATTT+AGG | 0.374759 | 1.1:-22948789 | MS.gene29253:CDS |
| GAAGAGAAGCTGAAAGAGAT+GGG | 0.482671 | 1.1:-22948754 | MS.gene29253:CDS |
| GGAAGAGAAGCTGAAAGAGA+TGG | 0.493039 | 1.1:-22948755 | MS.gene29253:CDS |
| AGAAGTAGCAGTTGCATCAG+TGG | 0.497636 | 1.1:+22948664 | None:intergenic |
| GAAAGAGATGGGTGTGGACG+AGG | 0.534995 | 1.1:-22948743 | MS.gene29253:CDS |
| GTGGACGAGGTTATAGTGAC+GGG | 0.562263 | 1.1:-22948730 | MS.gene29253:CDS |
| GAAGCTGAAAGAGATGGGTG+TGG | 0.582581 | 1.1:-22948749 | MS.gene29253:CDS |
| TTCTGATTTACATCTTCATG+AGG | 0.588358 | 1.1:-22948645 | MS.gene29253:CDS |
| TGTGGACGAGGTTATAGTGA+CGG | 0.588936 | 1.1:-22948731 | MS.gene29253:CDS |
| TACAACGCATTTAGGAACAC+AGG | 0.602970 | 1.1:-22948781 | MS.gene29253:CDS |
| TAACTTGTGTTGTGAGACAA+CGG | 0.702864 | 1.1:-22948698 | MS.gene29253:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| TTCTGATTTACATCTTCATG+AGG | - | chr1.1:22948793-22948812 | MS.gene29253:CDS | 30.0% | |
| AGAGTACATACAACGCATTT+AGG | - | chr1.1:22948649-22948668 | MS.gene29253:CDS | 35.0% | |
| ! | TAACTTGTGTTGTGAGACAA+CGG | - | chr1.1:22948740-22948759 | MS.gene29253:CDS | 35.0% |
| GAAGAGAAGCTGAAAGAGAT+GGG | - | chr1.1:22948684-22948703 | MS.gene29253:CDS | 40.0% | |
| TACAACGCATTTAGGAACAC+AGG | - | chr1.1:22948657-22948676 | MS.gene29253:CDS | 40.0% | |
| AGAAGTAGCAGTTGCATCAG+TGG | + | chr1.1:22948777-22948796 | None:intergenic | 45.0% | |
| CGCATTTAGGAACACAGGTT+TGG | - | chr1.1:22948662-22948681 | MS.gene29253:CDS | 45.0% | |
| GGAAGAGAAGCTGAAAGAGA+TGG | - | chr1.1:22948683-22948702 | MS.gene29253:CDS | 45.0% | |
| TGTGGACGAGGTTATAGTGA+CGG | - | chr1.1:22948707-22948726 | MS.gene29253:CDS | 45.0% | |
| GAAGCTGAAAGAGATGGGTG+TGG | - | chr1.1:22948689-22948708 | MS.gene29253:CDS | 50.0% | |
| GTGGACGAGGTTATAGTGAC+GGG | - | chr1.1:22948708-22948727 | MS.gene29253:CDS | 50.0% | |
| GAAAGAGATGGGTGTGGACG+AGG | - | chr1.1:22948695-22948714 | MS.gene29253:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.1 | gene | 22948636 | 22948824 | 22948636 | ID=MS.gene29253 |
| chr1.1 | mRNA | 22948636 | 22948824 | 22948636 | ID=MS.gene29253.t1;Parent=MS.gene29253 |
| chr1.1 | exon | 22948636 | 22948824 | 22948636 | ID=MS.gene29253.t1.exon1;Parent=MS.gene29253.t1 |
| chr1.1 | CDS | 22948636 | 22948824 | 22948636 | ID=cds.MS.gene29253.t1;Parent=MS.gene29253.t1 |
| Gene Sequence |
| Protein sequence |
