AlfalfaGEDB | MS.gene29427

M. sativa cultivar XinJiangDaYe / MS.gene29427

KEGG: K08829
GO: GO:0000166 GO:0003674 GO:0003824 GO:0004672 GO:0005488 GO:0005524 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0008144 GO:0008150 GO:0008152 GO:0009987 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017076 GO:0019538 GO:0030554 GO:0032553 GO:0032555 GO:0032559 GO:0035639 GO:0036094 GO:0036211 GO:0043167 GO:0043168 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0071704 GO:0097159 GO:0097367 GO:0140096 GO:1901265 GO:1901363 GO:1901564
PFAM: PF00069.28

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene29427.t1	XP_003612616.1	94.3	88	4	1	1	87	1	88	4.90E-37	163.7

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene29427.t1	Q6Z8C8	78.4	88	18	1	1	87	1	88	1.7e-33	143.3

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene29427.t1	G7K6V4	94.3	88	4	1	1	87	1	88	3.5e-37	163.7

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049142	MS.gene29427	0.816568	5.17E-52	-1.69E-46
MS.gene049647	MS.gene29427	0.821178	4.64E-53	-1.69E-46
MS.gene050941	MS.gene29427	0.802225	6.21E-49	-1.69E-46
MS.gene054479	MS.gene29427	0.804107	2.53E-49	-1.69E-46
MS.gene055926	MS.gene29427	0.802652	5.07E-49	-1.69E-46
MS.gene058758	MS.gene29427	0.813591	2.37E-51	-1.69E-46
MS.gene059283	MS.gene29427	0.806782	6.96E-50	-1.69E-46
MS.gene06010	MS.gene29427	0.80134	9.43E-49	-1.69E-46
MS.gene060191	MS.gene29427	0.800318	1.52E-48	-1.69E-46
MS.gene06052	MS.gene29427	0.811662	6.26E-51	-1.69E-46
MS.gene060998	MS.gene29427	0.819461	1.15E-52	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene29427.t1	MTR_5g026960	94.318	88	4	1	1	87	1	88	1.23e-53	173
MS.gene29427.t1	MTR_8g071230	85.227	88	12	1	1	87	1	88	6.26e-49	161
MS.gene29427.t1	MTR_8g012450	54.545	88	39	1	1	87	1	88	3.24e-29	108
MS.gene29427.t1	MTR_8g012450	54.545	88	39	1	1	87	1	88	3.39e-29	108
MS.gene29427.t1	MTR_3g054080	43.023	86	46	1	2	84	12	97	1.96e-15	70.9
MS.gene29427.t1	MTR_8g461040	42.391	92	47	2	3	88	25	116	5.23e-14	67.0
MS.gene29427.t1	MTR_6g013030	39.286	84	48	2	2	82	108	191	6.83e-14	66.6
MS.gene29427.t1	MTR_1g098300	37.931	87	49	3	2	84	124	209	1.83e-13	65.5
MS.gene29427.t1	MTR_4g109090	38.095	84	49	2	2	82	103	186	9.33e-13	63.2
MS.gene29427.t1	MTR_4g094430	36.145	83	52	1	1	82	1	83	1.24e-12	62.4
MS.gene29427.t1	MTR_2g072840	40.217	92	49	2	3	88	24	115	1.97e-12	62.4
MS.gene29427.t1	MTR_2g072840	40.217	92	49	2	3	88	24	115	2.01e-12	62.4
MS.gene29427.t1	MTR_2g032060	36.145	83	52	1	1	82	1	83	2.09e-12	62.0
MS.gene29427.t1	MTR_7g114300	36.905	84	50	2	2	82	105	188	2.23e-12	62.0
MS.gene29427.t1	MTR_7g114300	36.905	84	50	2	2	82	105	188	2.31e-12	62.0
MS.gene29427.t1	MTR_7g114300	36.905	84	50	2	2	82	105	188	2.41e-12	62.0
MS.gene29427.t1	MTR_7g114300	36.905	84	50	2	2	82	105	188	2.45e-12	62.0
MS.gene29427.t1	MTR_6g080470	39.535	86	47	2	2	84	12	95	6.16e-12	60.8
MS.gene29427.t1	MTR_5g013550	41.250	80	45	1	2	81	4	81	8.88e-12	60.5
MS.gene29427.t1	MTR_5g091680	35.870	92	53	2	3	88	21	112	5.54e-11	58.2
MS.gene29427.t1	MTR_5g091680	35.870	92	53	2	3	88	21	112	6.05e-11	58.2
MS.gene29427.t1	MTR_5g091680	35.870	92	53	2	3	88	19	110	6.13e-11	58.2
MS.gene29427.t1	MTR_5g091680	37.209	86	48	2	3	82	21	106	6.32e-11	58.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene29427.t1	AT4G19110	86.364	88	11	1	1	87	1	88	4.61e-51	167
MS.gene29427.t1	AT4G19110	86.364	88	11	1	1	87	1	88	4.73e-51	167
MS.gene29427.t1	AT5G45430	80.682	88	16	1	1	87	1	88	1.22e-46	156
MS.gene29427.t1	AT5G45430	80.682	88	16	1	1	87	1	88	1.24e-46	156
MS.gene29427.t1	AT4G13020	65.854	82	28	0	1	82	1	82	1.35e-36	128
MS.gene29427.t1	AT4G13020	65.854	82	28	0	1	82	1	82	1.37e-36	128
MS.gene29427.t1	AT4G13020	65.854	82	28	0	1	82	1	82	1.81e-36	128
MS.gene29427.t1	AT4G13020	65.854	82	28	0	1	82	1	82	2.31e-36	127
MS.gene29427.t1	AT4G13020	64.634	82	29	0	1	82	9	90	6.21e-35	124
MS.gene29427.t1	AT4G13020	64.634	82	29	0	1	82	9	90	6.29e-35	124
MS.gene29427.t1	AT4G13020	59.341	91	28	1	1	82	1	91	1.39e-33	120
MS.gene29427.t1	AT4G13020	59.341	91	28	1	1	82	1	91	1.89e-33	119
MS.gene29427.t1	AT5G39420	41.667	84	46	2	2	82	103	186	6.92e-14	66.6
MS.gene29427.t1	AT3G01085	39.286	84	48	2	2	82	113	196	1.16e-13	65.9
MS.gene29427.t1	AT3G01085	39.286	84	48	2	2	82	113	196	1.16e-13	65.9
MS.gene29427.t1	AT3G01085	39.286	84	48	2	2	82	113	196	1.16e-13	65.9
MS.gene29427.t1	AT3G14720	40.217	92	49	2	3	88	24	115	2.12e-13	65.1
MS.gene29427.t1	AT3G14720	41.860	86	44	2	3	82	24	109	5.75e-13	63.9
MS.gene29427.t1	AT3G14720	41.860	86	44	2	3	82	24	109	5.75e-13	63.9
MS.gene29427.t1	AT1G66750	39.080	87	48	2	1	84	10	94	9.23e-13	63.2
MS.gene29427.t1	AT1G67580	38.824	85	49	2	1	82	403	487	9.39e-13	63.5
MS.gene29427.t1	AT1G67580	38.824	85	49	2	1	82	403	487	9.39e-13	63.5
MS.gene29427.t1	AT3G48750	37.349	83	51	1	1	82	1	83	9.51e-13	62.8
MS.gene29427.t1	AT1G53510	39.130	92	50	2	3	88	24	115	1.01e-12	63.2
MS.gene29427.t1	AT1G71530	38.095	84	49	2	2	82	145	228	2.71e-12	62.0
MS.gene29427.t1	AT1G18670	36.905	84	50	2	2	82	129	212	2.73e-12	62.0
MS.gene29427.t1	AT1G18670	36.905	84	50	2	2	82	129	212	2.75e-12	62.0
MS.gene29427.t1	AT1G18670	36.905	84	50	2	2	82	129	212	2.75e-12	62.0
MS.gene29427.t1	AT1G71530	38.095	84	49	2	2	82	145	228	2.91e-12	62.0
MS.gene29427.t1	AT1G71530	38.095	84	49	2	2	82	145	228	2.94e-12	62.0
MS.gene29427.t1	AT1G71530	38.095	84	49	2	2	82	145	228	3.07e-12	62.0
MS.gene29427.t1	AT4G10010	38.095	84	49	2	2	82	154	237	4.43e-12	61.6
MS.gene29427.t1	AT4G19110	87.500	32	3	1	57	87	1	32	9.85e-12	60.5
MS.gene29427.t1	AT1G74330	35.714	84	51	2	2	82	119	202	1.13e-11	60.5
MS.gene29427.t1	AT1G74330	35.714	84	51	2	2	82	119	202	1.13e-11	60.5
MS.gene29427.t1	AT1G74330	35.714	84	51	2	2	82	119	202	1.13e-11	60.5
MS.gene29427.t1	AT3G18040	38.043	92	51	2	3	88	17	108	2.84e-11	58.9
MS.gene29427.t1	AT3G18040	38.043	92	51	2	3	88	22	113	2.85e-11	58.9
MS.gene29427.t1	AT3G18040	38.043	92	51	2	3	88	131	222	2.91e-11	58.9
MS.gene29427.t1	AT3G18040	38.043	92	51	2	3	88	131	222	2.92e-11	58.9
MS.gene29427.t1	AT2G42880	36.957	92	52	2	3	88	24	115	6.00e-11	58.2
MS.gene29427.t1	AT2G42880	36.957	92	52	2	3	88	24	115	6.19e-11	58.2
MS.gene29427.t1	AT5G19010	38.372	86	47	2	3	82	24	109	7.76e-11	57.8
MS.gene29427.t1	AT1G73690	37.209	86	49	2	2	84	9	92	8.61e-11	57.8

Find 23 sgRNAs with CRISPR-Local

Find 38 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TCCTTCAGTTTCACAATATT+TGG	0.029934	6.4:+25248877	None:intergenic
GTAATACCTTGACTTCTCTT+AGG	0.281840	6.4:+25249014	None:intergenic
GAAGTTGGTGATGGAACATT+TGG	0.328328	6.4:-25249210	MS.gene29427:CDS
CCTTCAGTTTCACAATATTT+GGG	0.336199	6.4:+25248878	None:intergenic
TGAAGAAGAAATATTACTCC+TGG	0.433782	6.4:-25249051	MS.gene29427:CDS
ATGGAACATTTGGGAGTGTC+TGG	0.443342	6.4:-25249200	MS.gene29427:CDS
TGTCCGACACAAACATGGTT+GGG	0.452336	6.4:+25248653	None:intergenic
AATCATACTTACAACTTCAC+CGG	0.516771	6.4:+25249158	None:intergenic
TACAAAATAATCAACGAAGT+TGG	0.522548	6.4:-25249225	MS.gene29427:CDS
TAGGTTTACGCACTCCTCCC+AGG	0.526948	6.4:+25249033	None:intergenic
AAGTTGGTGATGGAACATTT+GGG	0.545240	6.4:-25249209	MS.gene29427:CDS
TTGTCCGACACAAACATGGT+TGG	0.556533	6.4:+25248652	None:intergenic
AACATTGTCCGACACAAACA+TGG	0.557828	6.4:+25248648	None:intergenic
ACAAAAGACAGAATATCTAC+AGG	0.563192	6.4:-25248794	MS.gene29427:intron
ATAATCAACGAAGTTGGTGA+TGG	0.563332	6.4:-25249219	MS.gene29427:CDS
TCAGTTTCACAATATTTGGG+TGG	0.564489	6.4:+25248881	None:intergenic
GAAGAAGAAATATTACTCCT+GGG	0.573081	6.4:-25249050	MS.gene29427:CDS
CGTAAACCTAAGAGAAGTCA+AGG	0.573851	6.4:-25249020	MS.gene29427:intron
AGAGCTATTAGTAAGCAAAC+CGG	0.583714	6.4:-25249177	MS.gene29427:CDS
CCCAAATATTGTGAAACTGA+AGG	0.592543	6.4:-25248878	MS.gene29427:CDS
TGTTTGTGTCAGATATGTTG+TGG	0.613443	6.4:-25248689	MS.gene29427:intron
AATCCCAACCATGTTTGTGT+CGG	0.642986	6.4:-25248656	MS.gene29427:CDS
GAAGAAATATTACTCCTGGG+AGG	0.712706	6.4:-25249047	MS.gene29427:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	AAGTTGTAAGTATGATTTAC+TGG	-	chr6.4:25248808-25248827	MS.gene29427:CDS	25.0%
!	ATAACACATAACAACAACAA+AGG	+	chr6.4:25248993-25249012	None:intergenic	25.0%
!	TAATCAGTTACAAAATTTGC+AGG	-	chr6.4:25248871-25248890	MS.gene29427:CDS	25.0%
!	TACAAAATAATCAACGAAGT+TGG	-	chr6.4:25248735-25248754	MS.gene29427:intron	25.0%
!	TATACAACTCTTTATTAGGA+AGG	-	chr6.4:25249199-25249218	MS.gene29427:CDS	25.0%
!	TGTGTATACAACTCTTTATT+AGG	-	chr6.4:25249195-25249214	MS.gene29427:CDS	25.0%
!	TTACTTCATACTTATCTTTC+TGG	-	chr6.4:25248965-25248984	MS.gene29427:intron	25.0%
!!	TAATTTTCTCATTTACTGTG+TGG	-	chr6.4:25248679-25248698	MS.gene29427:CDS	25.0%
!!	TGCAAATTTTGTAACTGATT+AGG	+	chr6.4:25248872-25248891	None:intergenic	25.0%
	AATCATACTTACAACTTCAC+CGG	+	chr6.4:25248805-25248824	None:intergenic	30.0%
	ACAAAAGACAGAATATCTAC+AGG	-	chr6.4:25249166-25249185	MS.gene29427:intron	30.0%
	CCTTCAGTTTCACAATATTT+GGG	+	chr6.4:25249085-25249104	None:intergenic	30.0%
	GAAGAAGAAATATTACTCCT+GGG	-	chr6.4:25248910-25248929	MS.gene29427:intron	30.0%
	TCCTTCAGTTTCACAATATT+TGG	+	chr6.4:25249086-25249105	None:intergenic	30.0%
	TGAAGAAGAAATATTACTCC+TGG	-	chr6.4:25248909-25248928	MS.gene29427:intron	30.0%
!	GTAGATATTCTGTCTTTTGT+TGG	+	chr6.4:25249166-25249185	None:intergenic	30.0%
!!!	GTACTTTGTTTTTGAGTACA+TGG	-	chr6.4:25249130-25249149	MS.gene29427:intron	30.0%
	AGAGCTATTAGTAAGCAAAC+CGG	-	chr6.4:25248783-25248802	MS.gene29427:intron	35.0%
	CCCAAATATTGTGAAACTGA+AGG	-	chr6.4:25249082-25249101	MS.gene29427:intron	35.0%
	GTAATACCTTGACTTCTCTT+AGG	+	chr6.4:25248949-25248968	None:intergenic	35.0%
	TCAGTTTCACAATATTTGGG+TGG	+	chr6.4:25249082-25249101	None:intergenic	35.0%
	TGTTTGTGTCAGATATGTTG+TGG	-	chr6.4:25249271-25249290	MS.gene29427:intron	35.0%
!	ACATTTTTCCTTGACACTAC+AGG	-	chr6.4:25248712-25248731	MS.gene29427:intron	35.0%
!	ATTTTGTACCTGTAGTGTCA+AGG	+	chr6.4:25248723-25248742	None:intergenic	35.0%
!!	AAGTTGGTGATGGAACATTT+GGG	-	chr6.4:25248751-25248770	MS.gene29427:intron	35.0%
!!	ATAATCAACGAAGTTGGTGA+TGG	-	chr6.4:25248741-25248760	MS.gene29427:intron	35.0%
	AACATTGTCCGACACAAACA+TGG	+	chr6.4:25249315-25249334	None:intergenic	40.0%
	AATCCCAACCATGTTTGTGT+CGG	-	chr6.4:25249304-25249323	MS.gene29427:intron	40.0%
	CGTAAACCTAAGAGAAGTCA+AGG	-	chr6.4:25248940-25248959	MS.gene29427:intron	40.0%
	GAAGAAATATTACTCCTGGG+AGG	-	chr6.4:25248913-25248932	MS.gene29427:intron	40.0%
	TTCGTAAAGACTGCCAACAA+GGG	+	chr6.4:25249052-25249071	None:intergenic	40.0%
	TTTCGTAAAGACTGCCAACA+AGG	+	chr6.4:25249053-25249072	None:intergenic	40.0%
!	TGATCTCTTGTTTCCCTTGT+TGG	-	chr6.4:25249036-25249055	MS.gene29427:CDS	40.0%
!!	GAAGTTGGTGATGGAACATT+TGG	-	chr6.4:25248750-25248769	MS.gene29427:intron	40.0%
	TGTCCGACACAAACATGGTT+GGG	+	chr6.4:25249310-25249329	None:intergenic	45.0%
	TTGTCCGACACAAACATGGT+TGG	+	chr6.4:25249311-25249330	None:intergenic	45.0%
!!	ATGGAACATTTGGGAGTGTC+TGG	-	chr6.4:25248760-25248779	MS.gene29427:intron	45.0%
	TAGGTTTACGCACTCCTCCC+AGG	+	chr6.4:25248930-25248949	None:intergenic	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr6.4	gene	25248646	25249336	25248646	ID=MS.gene29427
chr6.4	mRNA	25248646	25249336	25248646	ID=MS.gene29427.t1;Parent=MS.gene29427
chr6.4	exon	25249329	25249336	25249329	ID=MS.gene29427.t1.exon1;Parent=MS.gene29427.t1
chr6.4	CDS	25249329	25249336	25249329	ID=cds.MS.gene29427.t1;Parent=MS.gene29427.t1
chr6.4	exon	25249170	25249248	25249170	ID=MS.gene29427.t1.exon2;Parent=MS.gene29427.t1
chr6.4	CDS	25249170	25249248	25249170	ID=cds.MS.gene29427.t1;Parent=MS.gene29427.t1
chr6.4	exon	25249021	25249089	25249021	ID=MS.gene29427.t1.exon3;Parent=MS.gene29427.t1
chr6.4	CDS	25249021	25249089	25249021	ID=cds.MS.gene29427.t1;Parent=MS.gene29427.t1
chr6.4	exon	25248795	25248920	25248795	ID=MS.gene29427.t1.exon4;Parent=MS.gene29427.t1
chr6.4	CDS	25248795	25248920	25248795	ID=cds.MS.gene29427.t1;Parent=MS.gene29427.t1
chr6.4	exon	25248646	25248699	25248646	ID=MS.gene29427.t1.exon5;Parent=MS.gene29427.t1
chr6.4	CDS	25248646	25248699	25248646	ID=cds.MS.gene29427.t1;Parent=MS.gene29427.t1

Gene Sequence

>MS.gene29427

ATGGAGAGGTGATTCATTTTCTGAAGAAATTACTAATTTTCTCATTTACTGTGTGGACTATTTTTAACATTTTTCCTTGACACTACAGGTACAAAATAATCAACGAAGTTGGTGATGGAACATTTGGGAGTGTCTGGAGAGCTATTAGTAAGCAAACCGGTGAAGTTGTAAGTATGATTTACTGGTATGTTGAAGTTTTCTCTATTTGAAGTGACATTTTTCTCCTAATCAGTTACAAAATTTGCAGGCTGCGATCAAGAAAATGAAGAAGAAATATTACTCCTGGGAGGAGTGCGTAAACCTAAGAGAAGTCAAGGTATTACTTCATACTTATCTTTCTGGAGCCTTTGTTGTTGTTATGTGTTATTATATTCTTTTGTATTTAGTCTCTGATCTCTTGTTTCCCTTGTTGGCAGTCTTTACGAAAAATGAACCACCCAAATATTGTGAAACTGAAGGAAGTTATTCGAGAAAGTGACATTTTGTACTTTGTTTTTGAGTACATGGTGACATTGTACCAACAAAAGACAGAATATCTACAGGTGTGTATGTGTATACAACTCTTTATTAGGAAGGTGACATTGTATTTCATGATTCAAGTCGAGTCACTCATGTTATTTCATTTTGTTTGTGTCAGATATGTTGTGGAATGCTGCTGAATCCCAACCATGTTTGTGTCGGACAATGTTAA

Protein sequence

>MS.gene29427.t1

MERYKIINEVGDGTFGSVWRAISKQTGEVAAIKKMKKKYYSWEECVNLREVKSLRKMNHPNIVKLKEVIRESDILYFVFEYMVTLYQQKTEYLQICCGMLLNPNHVCVGQC