Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene29479.t1 | XP_003616397.1 | 99 | 391 | 4 | 0 | 1 | 391 | 1 | 391 | 2.10E-223 | 784.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene29479.t1 | Q9Y312 | 28.8 | 400 | 245 | 9 | 1 | 386 | 6 | 379 | 5.6e-36 | 153.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene29479.t1 | G7KBQ2 | 99.0 | 391 | 4 | 0 | 1 | 391 | 1 | 391 | 1.5e-223 | 784.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene29479 | MS.gene038800 | PPI |
| MS.gene29479 | MS.gene044498 | PPI |
| MS.gene68979 | MS.gene29479 | PPI |
| MS.gene29479 | MS.gene033017 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene29479.t1 | MTR_5g079770 | 98.977 | 391 | 4 | 0 | 1 | 391 | 1 | 391 | 0.0 | 800 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene29479.t1 | AT1G66510 | 67.758 | 397 | 116 | 5 | 1 | 390 | 1 | 392 | 0.0 | 563 |
| MS.gene29479.t1 | AT1G66510 | 67.758 | 397 | 116 | 5 | 1 | 390 | 1 | 392 | 0.0 | 563 |
| MS.gene29479.t1 | AT1G66510 | 67.758 | 397 | 116 | 5 | 1 | 390 | 1 | 392 | 0.0 | 563 |
| MS.gene29479.t1 | AT1G66510 | 66.006 | 353 | 108 | 5 | 45 | 390 | 1 | 348 | 8.42e-174 | 489 |
Find 84 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGCAGGTAATTGTTCGTAAA+TGG | 0.186934 | 5.3:+64509038 | MS.gene29479:intron |
| ACTTTACTGGCAAAAGATTA+TGG | 0.257738 | 5.3:+64511110 | MS.gene29479:CDS |
| CACTATTGTTGGATGATTCT+TGG | 0.258501 | 5.3:+64512690 | MS.gene29479:CDS |
| AAGCTATTAGAGAGCAATCT+AGG | 0.318940 | 5.3:+64513340 | MS.gene29479:CDS |
| GTTTGCATTTATCGCCCTTA+TGG | 0.327929 | 5.3:+64511166 | MS.gene29479:CDS |
| CAGCAGAACAACGCTGTTGA+TGG | 0.346353 | 5.3:+64513373 | MS.gene29479:CDS |
| TCCTTGGTTAGCCTTCTGTT+TGG | 0.383792 | 5.3:+64511302 | MS.gene29479:CDS |
| ATTGGATTCTTTATTGATGC+TGG | 0.406561 | 5.3:+64507699 | MS.gene29479:CDS |
| ATTGTTTACCTGAGTATCAA+TGG | 0.419994 | 5.3:-64505238 | None:intergenic |
| ATGGAATTTGACCGACAACT+TGG | 0.429503 | 5.3:+64509836 | MS.gene29479:CDS |
| CTAGATGGGTCTGTCATTGA+TGG | 0.429928 | 5.3:+64513127 | MS.gene29479:CDS |
| ACAATTGCTAGAGACTTTAC+TGG | 0.432701 | 5.3:+64511097 | MS.gene29479:CDS |
| ATCTTAGCCACTATGAAGAT+TGG | 0.447384 | 5.3:+64509867 | MS.gene29479:CDS |
| TAGTAGACAAAATGAGTTCC+CGG | 0.450062 | 5.3:-64506941 | None:intergenic |
| TGTTCGTAAATGGGACCAAC+AGG | 0.453079 | 5.3:+64509048 | MS.gene29479:CDS |
| GTTGGCAAGTTGCAGAGAAA+AGG | 0.455685 | 5.3:+64510133 | MS.gene29479:CDS |
| GCAGGTAATTGTTCGTAAAT+GGG | 0.457276 | 5.3:+64509039 | MS.gene29479:intron |
| ATCTATTATCAACTAAAATA+CGG | 0.457832 | 5.3:+64512635 | MS.gene29479:CDS |
| GGTTCTGAAGACTTGCTCCT+TGG | 0.470331 | 5.3:+64511134 | MS.gene29479:CDS |
| TCATAGTGGCTAAGATTGTA+AGG | 0.472984 | 5.3:-64509860 | None:intergenic |
| TGACTAGCCGTTCCTCCTGT+TGG | 0.475000 | 5.3:-64509063 | None:intergenic |
| AACACTGACTTCTCCTCCAA+TGG | 0.477819 | 5.3:-64510017 | None:intergenic |
| AGAAAATATACTTACTGCTT+CGG | 0.477936 | 5.3:-64511330 | None:intergenic |
| GATAATTCTGCAACATCTGT+TGG | 0.478381 | 5.3:+64510115 | MS.gene29479:CDS |
| CTAGGATGAAAGATATAGTC+TGG | 0.482454 | 5.3:+64509802 | MS.gene29479:intron |
| GGGTATTAAGATGATTCCTC+CGG | 0.487283 | 5.3:+64506922 | MS.gene29479:CDS |
| ATTTCTCCAGTGGAAATCCT+TGG | 0.497323 | 5.3:+64511286 | MS.gene29479:CDS |
| GTGTCTAGTGCTTTCTCCAT+TGG | 0.503391 | 5.3:-64510079 | None:intergenic |
| GGCTTCCTGCAGAGATGGAA+AGG | 0.504025 | 5.3:+64507662 | MS.gene29479:intron |
| GATTGTAAGGCCCAAGTTGT+CGG | 0.506606 | 5.3:-64509847 | None:intergenic |
| TATTGATGCTGGCCCTTCAG+AGG | 0.511306 | 5.3:+64507710 | MS.gene29479:CDS |
| TAAATAATACCTTACAATGA+TGG | 0.519108 | 5.3:-64512736 | None:intergenic |
| TTGGCAAGTTGCAGAGAAAA+GGG | 0.521046 | 5.3:+64510134 | MS.gene29479:CDS |
| TATGGATTCTCAAACAGCGT+TGG | 0.521890 | 5.3:+64505155 | None:intergenic |
| GATCAAATCAAACCTGGTTG+AGG | 0.524670 | 5.3:-64506969 | None:intergenic |
| AGTCAAAGTATCTGAGGAAG+AGG | 0.525053 | 5.3:+64509081 | MS.gene29479:CDS |
| TCATCTAACATCTCTACTAC+AGG | 0.528298 | 5.3:-64513517 | None:intergenic |
| TAACACAGGACCACTATTGT+TGG | 0.533398 | 5.3:+64512679 | MS.gene29479:CDS |
| CCTGAGTATCAATGGCAACA+AGG | 0.534352 | 5.3:-64505230 | None:intergenic |
| TGGAATTTGACCGACAACTT+GGG | 0.538353 | 5.3:+64509837 | MS.gene29479:CDS |
| ACACTGACTTCTCCTCCAAT+GGG | 0.539146 | 5.3:-64510016 | None:intergenic |
| CAAGAATCATCCAACAATAG+TGG | 0.539982 | 5.3:-64512689 | None:intergenic |
| CCTTGTTGCCATTGATACTC+AGG | 0.546257 | 5.3:+64505230 | MS.gene29479:CDS |
| GGCTAACCAAGGATTTCCAC+TGG | 0.549600 | 5.3:-64511292 | None:intergenic |
| ATCTTAATACCCTTAAAAGA+AGG | 0.553188 | 5.3:-64506911 | None:intergenic |
| TATTTATGAGCAGAGCCCAT+TGG | 0.553357 | 5.3:+64510001 | MS.gene29479:intron |
| CCACGTGTTGTAAAATGCAA+GGG | 0.556834 | 5.3:+64510175 | MS.gene29479:CDS |
| TTATGAGCAGAGCCCATTGG+AGG | 0.557137 | 5.3:+64510004 | MS.gene29479:intron |
| ATGCAAGGGGATCAGTGGAC+AGG | 0.557605 | 5.3:+64510189 | MS.gene29479:CDS |
| AGCTATTAGAGAGCAATCTA+GGG | 0.559485 | 5.3:+64513341 | MS.gene29479:CDS |
| TCCACGTGTTGTAAAATGCA+AGG | 0.560327 | 5.3:+64510174 | MS.gene29479:CDS |
| AAGAGCATCATCGAACGGCT+TGG | 0.567694 | 5.3:+64509914 | MS.gene29479:CDS |
| ATCGTTTCCAATCTTCATAG+TGG | 0.569850 | 5.3:-64509874 | None:intergenic |
| CGTTAGAAGCATTTCTCCAG+TGG | 0.577996 | 5.3:+64511276 | MS.gene29479:CDS |
| TCAATAAAGAATCCAATCAT+CGG | 0.579744 | 5.3:-64507693 | None:intergenic |
| TCTACTATAGTTCCTCAACC+AGG | 0.581273 | 5.3:+64506957 | MS.gene29479:CDS |
| CTGAGTATCAATGGCAACAA+GGG | 0.594931 | 5.3:-64505229 | None:intergenic |
| CTTCGGTGCAGCCAAACAGA+AGG | 0.598687 | 5.3:-64511313 | None:intergenic |
| AAACGCTCCTAAAACACCAA+TGG | 0.600774 | 5.3:+64510063 | MS.gene29479:CDS |
| TATTAGAGAGCAATCTAGGG+TGG | 0.601228 | 5.3:+64513344 | MS.gene29479:CDS |
| AGCACTAGACACGCAGCTGA+AGG | 0.603110 | 5.3:+64510090 | MS.gene29479:CDS |
| AATGGGACCAACAGGAGGAA+CGG | 0.609015 | 5.3:+64509056 | MS.gene29479:CDS |
| GGTATTAAGATGATTCCTCC+GGG | 0.612119 | 5.3:+64506923 | MS.gene29479:CDS |
| TGGCAACAAGGGTGTACTGA+GGG | 0.612425 | 5.3:-64505218 | None:intergenic |
| GTAAAATGCAAGGGGATCAG+TGG | 0.615109 | 5.3:+64510184 | MS.gene29479:CDS |
| ATTAGAGAGCAATCTAGGGT+GGG | 0.617313 | 5.3:+64513345 | MS.gene29479:CDS |
| ATGCAAACTGCAGTTCTCCA+AGG | 0.619224 | 5.3:-64511151 | None:intergenic |
| TTACAAAGAGCATCATCGAA+CGG | 0.632571 | 5.3:+64509909 | MS.gene29479:CDS |
| AGCCTTTCCACACACGAACA+CGG | 0.635124 | 5.3:+64512060 | MS.gene29479:intron |
| TAAATAGCCGTGTTCGTGTG+TGG | 0.637252 | 5.3:-64512067 | None:intergenic |
| CGGACTACAGAAAGATCGCA+AGG | 0.640325 | 5.3:+64512655 | MS.gene29479:CDS |
| ACGGCTAGTCAAAGTATCTG+AGG | 0.646658 | 5.3:+64509075 | MS.gene29479:CDS |
| CACGTGTTGTAAAATGCAAG+GGG | 0.646735 | 5.3:+64510176 | MS.gene29479:CDS |
| TACTTCTTTGAATCTTGACA+AGG | 0.647721 | 5.3:+64510216 | MS.gene29479:CDS |
| AGCCGTGTTCGTGTGTGGAA+AGG | 0.649513 | 5.3:-64512062 | None:intergenic |
| GCCAAACAGAAGGCTAACCA+AGG | 0.675388 | 5.3:-64511303 | None:intergenic |
| GTACTTTGATGAGAACGATG+AGG | 0.679398 | 5.3:+64513399 | MS.gene29479:CDS |
| TCGTAAATGGGACCAACAGG+AGG | 0.680154 | 5.3:+64509051 | MS.gene29479:CDS |
| ATGGCAACAAGGGTGTACTG+AGG | 0.692263 | 5.3:-64505219 | None:intergenic |
| AAAGATCGCAAGGATAACAC+AGG | 0.694703 | 5.3:+64512665 | MS.gene29479:CDS |
| TTACTGGCAAAAGATTATGG+AGG | 0.700059 | 5.3:+64511113 | MS.gene29479:CDS |
| ACGAACACGGCTATTTACCA+AGG | 0.703530 | 5.3:+64512073 | MS.gene29479:CDS |
| GGGTGTACTGAGGGACATCG+AGG | 0.709510 | 5.3:-64505209 | None:intergenic |
| TAGACAAAATGAGTTCCCGG+AGG | 0.766673 | 5.3:-64506938 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.3 | gene | 64505156 | 64513558 | 64505156 | ID=MS.gene29479 |
| chr5.3 | mRNA | 64505156 | 64513558 | 64505156 | ID=MS.gene29479.t1;Parent=MS.gene29479 |
| chr5.3 | exon | 64505156 | 64505251 | 64505156 | ID=MS.gene29479.t1.exon1;Parent=MS.gene29479.t1 |
| chr5.3 | CDS | 64505156 | 64505251 | 64505156 | ID=cds.MS.gene29479.t1;Parent=MS.gene29479.t1 |
| chr5.3 | exon | 64506896 | 64506978 | 64506896 | ID=MS.gene29479.t1.exon2;Parent=MS.gene29479.t1 |
| chr5.3 | CDS | 64506896 | 64506978 | 64506896 | ID=cds.MS.gene29479.t1;Parent=MS.gene29479.t1 |
| chr5.3 | exon | 64507674 | 64507731 | 64507674 | ID=MS.gene29479.t1.exon3;Parent=MS.gene29479.t1 |
| chr5.3 | CDS | 64507674 | 64507731 | 64507674 | ID=cds.MS.gene29479.t1;Parent=MS.gene29479.t1 |
| chr5.3 | exon | 64509043 | 64509102 | 64509043 | ID=MS.gene29479.t1.exon4;Parent=MS.gene29479.t1 |
| chr5.3 | CDS | 64509043 | 64509102 | 64509043 | ID=cds.MS.gene29479.t1;Parent=MS.gene29479.t1 |
| chr5.3 | exon | 64509806 | 64509935 | 64509806 | ID=MS.gene29479.t1.exon5;Parent=MS.gene29479.t1 |
| chr5.3 | CDS | 64509806 | 64509935 | 64509806 | ID=cds.MS.gene29479.t1;Parent=MS.gene29479.t1 |
| chr5.3 | exon | 64510014 | 64510237 | 64510014 | ID=MS.gene29479.t1.exon6;Parent=MS.gene29479.t1 |
| chr5.3 | CDS | 64510014 | 64510237 | 64510014 | ID=cds.MS.gene29479.t1;Parent=MS.gene29479.t1 |
| chr5.3 | exon | 64511095 | 64511187 | 64511095 | ID=MS.gene29479.t1.exon7;Parent=MS.gene29479.t1 |
| chr5.3 | CDS | 64511095 | 64511187 | 64511095 | ID=cds.MS.gene29479.t1;Parent=MS.gene29479.t1 |
| chr5.3 | exon | 64511266 | 64511337 | 64511266 | ID=MS.gene29479.t1.exon8;Parent=MS.gene29479.t1 |
| chr5.3 | CDS | 64511266 | 64511337 | 64511266 | ID=cds.MS.gene29479.t1;Parent=MS.gene29479.t1 |
| chr5.3 | exon | 64512062 | 64512094 | 64512062 | ID=MS.gene29479.t1.exon9;Parent=MS.gene29479.t1 |
| chr5.3 | CDS | 64512062 | 64512094 | 64512062 | ID=cds.MS.gene29479.t1;Parent=MS.gene29479.t1 |
| chr5.3 | exon | 64512623 | 64512748 | 64512623 | ID=MS.gene29479.t1.exon10;Parent=MS.gene29479.t1 |
| chr5.3 | CDS | 64512623 | 64512748 | 64512623 | ID=cds.MS.gene29479.t1;Parent=MS.gene29479.t1 |
| chr5.3 | exon | 64513109 | 64513165 | 64513109 | ID=MS.gene29479.t1.exon11;Parent=MS.gene29479.t1 |
| chr5.3 | CDS | 64513109 | 64513165 | 64513109 | ID=cds.MS.gene29479.t1;Parent=MS.gene29479.t1 |
| chr5.3 | exon | 64513325 | 64513420 | 64513325 | ID=MS.gene29479.t1.exon12;Parent=MS.gene29479.t1 |
| chr5.3 | CDS | 64513325 | 64513420 | 64513325 | ID=cds.MS.gene29479.t1;Parent=MS.gene29479.t1 |
| chr5.3 | exon | 64513511 | 64513558 | 64513511 | ID=MS.gene29479.t1.exon13;Parent=MS.gene29479.t1 |
| chr5.3 | CDS | 64513511 | 64513558 | 64513511 | ID=cds.MS.gene29479.t1;Parent=MS.gene29479.t1 |
| Gene Sequence |
| Protein sequence |
