AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene038800


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene038800.t1 MTR_4g112780 97.452 157 0 1 1 157 1 153 1.26e-106 301
MS.gene038800.t1 MTR_4g114330 34.483 116 75 1 21 136 4 118 2.17e-14 66.2
MS.gene038800.t1 MTR_5g077600 72.727 44 11 1 105 148 23 65 6.86e-14 63.2
MS.gene038800.t1 MTR_0011s0330 34.821 112 72 1 25 136 8 118 2.09e-13 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene038800.t1 AT5G08180 68.153 157 49 1 1 157 1 156 4.91e-76 224
MS.gene038800.t1 AT5G08180 68.153 157 49 1 1 157 1 156 4.91e-76 224
MS.gene038800.t1 AT4G12600 34.188 117 76 1 20 136 3 118 5.16e-13 62.4
MS.gene038800.t1 AT5G20160 35.714 112 71 1 25 136 8 118 5.27e-13 62.4
MS.gene038800.t1 AT4G22380 34.821 112 72 1 25 136 8 118 9.05e-13 62.0

Find 32 sgRNAs with CRISPR-Local

Find 105 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGTATAAGGCGAGGTCAAAA+AGG 0.296737 8.3:+14716296 MS.gene038800:CDS
CCCATTGCAAAACCTCTTGC+TGG 0.348880 8.3:+14715459 MS.gene038800:CDS
TGAGATAGTTCACCCTTTGA+AGG 0.372203 8.3:-14717349 None:intergenic
AGGAGGTGGTTAAAAGTATA+AGG 0.394940 8.3:+14716282 MS.gene038800:CDS
GTGCATAAAGAGAGGAGTTA+AGG 0.402782 8.3:+14716262 MS.gene038800:CDS
TCCTTATCCTCGCATAAAAT+CGG 0.425786 8.3:-14716554 None:intergenic
TGCAGAGTGTGTATTATAGC+TGG 0.425932 8.3:+14716497 MS.gene038800:intron
TAGTTCACCCTTTGAAGGCT+TGG 0.444404 8.3:-14717344 None:intergenic
ACATGAGTGATGACATCAAT+AGG 0.449731 8.3:-14716530 None:intergenic
GTCCAGGACCTTGCAACGGC+AGG 0.468819 8.3:+14717283 MS.gene038800:intron
TTAGATGAGACATACACATA+TGG 0.472823 8.3:-14716581 None:intergenic
CGACAGTGAAGGTGAGAAAT+CGG 0.483487 8.3:+14715395 MS.gene038800:CDS
GTGATGACCAAGCCTTCAAA+GGG 0.500756 8.3:+14717337 MS.gene038800:CDS
TCGTTGCTCCTGCCGTTGCA+AGG 0.501816 8.3:-14717291 None:intergenic
AGTGATGACCAAGCCTTCAA+AGG 0.525648 8.3:+14717336 MS.gene038800:CDS
ACACTCAAGCTTGTTAAAAG+AGG 0.527156 8.3:+14715501 MS.gene038800:CDS
AAAGAGAGGAGTTAAGGAGG+TGG 0.543156 8.3:+14716268 MS.gene038800:CDS
CGGTGCAGAGGGAGAAGGAG+AGG 0.549279 8.3:+14715415 MS.gene038800:CDS
CATAAAGAGAGGAGTTAAGG+AGG 0.557458 8.3:+14716265 MS.gene038800:CDS
GAAAGCTTCTTTCCAGCAAG+AGG 0.560382 8.3:-14715471 None:intergenic
GCAGAGTGTGTATTATAGCT+GGG 0.568819 8.3:+14716498 MS.gene038800:intron
ATCACTAAAACACAACAAGT+TGG 0.571248 8.3:-14717319 None:intergenic
GAAGGAGAGGAAGAAGATGC+AGG 0.571568 8.3:+14715428 MS.gene038800:CDS
GAAATCGGTGCAGAGGGAGA+AGG 0.585599 8.3:+14715410 MS.gene038800:CDS
AAGGTGAGAAATCGGTGCAG+AGG 0.589190 8.3:+14715403 MS.gene038800:CDS
AGGTGAGAAATCGGTGCAGA+GGG 0.594395 8.3:+14715404 MS.gene038800:CDS
AAAGGGTGAACTATCTCAAG+AGG 0.620179 8.3:+14717354 MS.gene038800:CDS
GAAAACAAGTGCATAAAGAG+AGG 0.625540 8.3:+14716254 MS.gene038800:CDS
ATTTGTCCAGGACCTTGCAA+CGG 0.636321 8.3:+14717279 MS.gene038800:intron
CAACGGCAGGAGCAACGAAG+AGG 0.638950 8.3:+14717296 MS.gene038800:CDS
GAAATGGGAAGCGACAGTGA+AGG 0.681973 8.3:+14715384 None:intergenic
GTGGTTAAAAGTATAAGGCG+AGG 0.693539 8.3:+14716287 MS.gene038800:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ACAATTGAAACATAAAAAAT+GGG - chr8.3:14716436-14716455 None:intergenic 15.0%
!! TTATAAGCTATAAACTATAT+TGG + chr8.3:14715874-14715893 MS.gene038800:intron 15.0%
!! TTCCAAAATAAAAGAAAAAA+CGG - chr8.3:14716103-14716122 None:intergenic 15.0%
!!! TTTTTTCTTTTATTTTGGAA+TGG + chr8.3:14716103-14716122 MS.gene038800:intron 15.0%
!! ATAGCTTATAAAAACAACTT+AGG - chr8.3:14715865-14715884 None:intergenic 20.0%
!! ATTCATAACATAACATTCTT+GGG - chr8.3:14716015-14716034 None:intergenic 20.0%
!! CACAATTGAAACATAAAAAA+TGG - chr8.3:14716437-14716456 None:intergenic 20.0%
!! CTATTGTATTCATAATATAC+AGG - chr8.3:14717019-14717038 None:intergenic 20.0%
!! GAATTGTTGATATATTATGT+AGG - chr8.3:14717091-14717110 None:intergenic 20.0%
!! TACATAAATCTTTGTCTTAT+GGG + chr8.3:14716197-14716216 MS.gene038800:intron 20.0%
!! TTACATAAATCTTTGTCTTA+TGG + chr8.3:14716196-14716215 MS.gene038800:intron 20.0%
!!! CATTTTTTATGTTTCAATTG+TGG + chr8.3:14716435-14716454 MS.gene038800:intron 20.0%
!!! GTTGATTCTGTAATTTAAAA+AGG + chr8.3:14716139-14716158 MS.gene038800:intron 20.0%
!!! TAATGAAAATCGTTAATTTG+TGG + chr8.3:14715561-14715580 MS.gene038800:intron 20.0%
!!! TTTCAGTTTCGTTTTAAATA+TGG + chr8.3:14717123-14717142 MS.gene038800:intron 20.0%
! AATAAGTTGTTTATCCAAAC+AGG - chr8.3:14715741-14715760 None:intergenic 25.0%
! ATATGGTATTAAGAGAAACA+TGG + chr8.3:14717140-14717159 MS.gene038800:intron 25.0%
! GATTCATAACATAACATTCT+TGG - chr8.3:14716016-14716035 None:intergenic 25.0%
! TCATAAATAACCAAAAGTTG+AGG + chr8.3:14716755-14716774 MS.gene038800:intron 25.0%
! TTCATAACATAACATTCTTG+GGG - chr8.3:14716014-14716033 None:intergenic 25.0%
! TTCCATTCAAATATGTTACA+TGG - chr8.3:14716879-14716898 None:intergenic 25.0%
!! CTTATTCTAAAGAGAGATTA+GGG - chr8.3:14716681-14716700 None:intergenic 25.0%
!! TCTTATTCTAAAGAGAGATT+AGG - chr8.3:14716682-14716701 None:intergenic 25.0%
!! TTTCAAAGTACTAGAGTAAT+TGG - chr8.3:14717235-14717254 None:intergenic 25.0%
!!! ATGAACTTGTTATGGAATTA+AGG + chr8.3:14715714-14715733 MS.gene038800:intron 25.0%
!!! CTTCAAAAATGAACTTGTTA+TGG + chr8.3:14715706-14715725 MS.gene038800:intron 25.0%
!!! TGAACTTGTTATGGAATTAA+GGG + chr8.3:14715715-14715734 MS.gene038800:intron 25.0%
!!! TGCCGTTTTTTCTTTTATTT+TGG + chr8.3:14716098-14716117 MS.gene038800:intron 25.0%
AAACATTAGATTGCTCACAA+AGG + chr8.3:14716333-14716352 MS.gene038800:intron 30.0%
AAATCTGCTATGCAATCATT+TGG + chr8.3:14717185-14717204 MS.gene038800:intron 30.0%
AACATTAGATTGCTCACAAA+GGG + chr8.3:14716334-14716353 MS.gene038800:intron 30.0%
ATCACTAAAACACAACAAGT+TGG - chr8.3:14717322-14717341 None:intergenic 30.0%
ATGGTAAAAAGACAGCAAAA+AGG - chr8.3:14716068-14716087 None:intergenic 30.0%
GAGAAGAAGGCAAAAAATAA+GGG - chr8.3:14716475-14716494 None:intergenic 30.0%
GTCCATGTAACATATTTGAA+TGG + chr8.3:14716874-14716893 MS.gene038800:intron 30.0%
TATGACATCAAATTAACCTC+AGG - chr8.3:14716740-14716759 None:intergenic 30.0%
TCATATCTTCCTTGACAAAA+TGG + chr8.3:14716394-14716413 MS.gene038800:intron 30.0%
TGAGAAGAAGGCAAAAAATA+AGG - chr8.3:14716476-14716495 None:intergenic 30.0%
TTAGATGAGACATACACATA+TGG - chr8.3:14716584-14716603 None:intergenic 30.0%
! ATAACACAAATTGTGTTGAC+AGG + chr8.3:14716845-14716864 MS.gene038800:intron 30.0%
! ATTGAGTAACCATTTTGTCA+AGG - chr8.3:14716406-14716425 None:intergenic 30.0%
! GAATGTATGCTTTTCTATAC+TGG + chr8.3:14716896-14716915 MS.gene038800:intron 30.0%
! GCTTGAATGTTTTGTCAATT+TGG + chr8.3:14716172-14716191 MS.gene038800:intron 30.0%
!! AATTTCTGAGATATTTGTCC+AGG + chr8.3:14717267-14717286 MS.gene038800:intron 30.0%
AAAACGGCAGCAAATTTGTA+TGG - chr8.3:14716087-14716106 None:intergenic 35.0%
ACATGAGTGATGACATCAAT+AGG - chr8.3:14716533-14716552 None:intergenic 35.0%
AGCACTCAAGTTACAAAACA+AGG - chr8.3:14716806-14716825 None:intergenic 35.0%
CACAGTTTATCGAGAAAGAA+AGG + chr8.3:14717045-14717064 MS.gene038800:intron 35.0%
CTAAGAATTGCAGTGTTTCT+GGG + chr8.3:14715587-14715606 MS.gene038800:intron 35.0%
GAAAACAAGTGCATAAAGAG+AGG + chr8.3:14716254-14716273 MS.gene038800:CDS 35.0%
GGAGAGTTCTTGAAAATAAG+TGG + chr8.3:14715895-14715914 MS.gene038800:intron 35.0%
TAAAGAGAGATTAGGGAAAC+AGG - chr8.3:14716674-14716693 None:intergenic 35.0%
TAATATACAGGTCTATGAGC+TGG - chr8.3:14717007-14717026 None:intergenic 35.0%
TCCTTATCCTCGCATAAAAT+CGG - chr8.3:14716557-14716576 None:intergenic 35.0%
TCTAAGAATTGCAGTGTTTC+TGG + chr8.3:14715586-14715605 MS.gene038800:intron 35.0%
TGAAATAAGCCAATCCAAAC+AGG + chr8.3:14715988-14716007 MS.gene038800:intron 35.0%
TTTCTTACACATATAGCCTG+AGG + chr8.3:14716721-14716740 MS.gene038800:intron 35.0%
! AACAAGCTTGAGTGTTTTCT+TGG - chr8.3:14715496-14715515 None:intergenic 35.0%
! AGGAGGTGGTTAAAAGTATA+AGG + chr8.3:14716282-14716301 MS.gene038800:CDS 35.0%
!! ACACTCAAGCTTGTTAAAAG+AGG + chr8.3:14715501-14715520 MS.gene038800:CDS 35.0%
AAAGGGTGAACTATCTCAAG+AGG + chr8.3:14717354-14717373 MS.gene038800:CDS 40.0%
AACTTTGCGCTGAATCTTGA+TGG + chr8.3:14716618-14716637 MS.gene038800:intron 40.0%
AAGGTGCAACATCATCAATG+AGG - chr8.3:14716787-14716806 None:intergenic 40.0%
AGTATAAGGCGAGGTCAAAA+AGG + chr8.3:14716296-14716315 MS.gene038800:CDS 40.0%
ATCAAGATTCAGCGCAAAGT+TGG - chr8.3:14716619-14716638 None:intergenic 40.0%
CATAAAGAGAGGAGTTAAGG+AGG + chr8.3:14716265-14716284 MS.gene038800:CDS 40.0%
GCAGAGTGTGTATTATAGCT+GGG + chr8.3:14716498-14716517 MS.gene038800:intron 40.0%
GCTTATTTCAGCTGATCTAC+TGG - chr8.3:14715978-14715997 None:intergenic 40.0%
GTATAAGGCGAGGTCAAAAA+GGG + chr8.3:14716297-14716316 MS.gene038800:CDS 40.0%
GTGCATAAAGAGAGGAGTTA+AGG + chr8.3:14716262-14716281 MS.gene038800:CDS 40.0%
GTGTTATGAATGCCAGCATA+AGG - chr8.3:14716832-14716851 None:intergenic 40.0%
TATGGAATTAAGGGCCTGTT+TGG + chr8.3:14715724-14715743 MS.gene038800:intron 40.0%
TGAGATAGTTCACCCTTTGA+AGG - chr8.3:14717352-14717371 None:intergenic 40.0%
TGCAGAGTGTGTATTATAGC+TGG + chr8.3:14716497-14716516 MS.gene038800:intron 40.0%
! GAGGAAAAAGCCTCAACTTT+TGG - chr8.3:14716768-14716787 None:intergenic 40.0%
! GTGGTTAAAAGTATAAGGCG+AGG + chr8.3:14716287-14716306 MS.gene038800:CDS 40.0%
! TCATGTTCCGATTTTATGCG+AGG + chr8.3:14716547-14716566 MS.gene038800:CDS 40.0%
! TCCGATTTTATGCGAGGATA+AGG + chr8.3:14716553-14716572 MS.gene038800:CDS 40.0%
! TTGAGTGCTTTTCCTTATGC+TGG + chr8.3:14716817-14716836 MS.gene038800:intron 40.0%
!!! TTGCAGTGTTTCTGGGTTTT+TGG + chr8.3:14715594-14715613 MS.gene038800:intron 40.0%
AAAGAGAGGAGTTAAGGAGG+TGG + chr8.3:14716268-14716287 MS.gene038800:CDS 45.0%
AGAGAGATTAGGGAAACAGG+CGG - chr8.3:14716671-14716690 None:intergenic 45.0%
AGTGATGACCAAGCCTTCAA+AGG + chr8.3:14717336-14717355 MS.gene038800:CDS 45.0%
ATTTGTCCAGGACCTTGCAA+CGG + chr8.3:14717279-14717298 MS.gene038800:intron 45.0%
CACACTCTGCATTGAGAAGA+AGG - chr8.3:14716488-14716507 None:intergenic 45.0%
CGACAGTGAAGGTGAGAAAT+CGG + chr8.3:14715395-14715414 MS.gene038800:CDS 45.0%
GAAAGCTTCTTTCCAGCAAG+AGG - chr8.3:14715474-14715493 None:intergenic 45.0%
GTGATGACCAAGCCTTCAAA+GGG + chr8.3:14717337-14717356 MS.gene038800:CDS 45.0%
TAACATTCTTGGGGCCTGTT+TGG - chr8.3:14716005-14716024 None:intergenic 45.0%
! AGCAAGAGGTTTTGCAATGG+GGG - chr8.3:14715460-14715479 None:intergenic 45.0%
! CAGCAAGAGGTTTTGCAATG+GGG - chr8.3:14715461-14715480 None:intergenic 45.0%
! CCAGCAAGAGGTTTTGCAAT+GGG - chr8.3:14715462-14715481 None:intergenic 45.0%
! TAGTTCACCCTTTGAAGGCT+TGG - chr8.3:14717347-14717366 None:intergenic 45.0%
! TCCAGCAAGAGGTTTTGCAA+TGG - chr8.3:14715463-14715482 None:intergenic 45.0%
AAGGTGAGAAATCGGTGCAG+AGG + chr8.3:14715403-14715422 MS.gene038800:CDS 50.0%
AGGTGAGAAATCGGTGCAGA+GGG + chr8.3:14715404-14715423 MS.gene038800:CDS 50.0%
CCCATTGCAAAACCTCTTGC+TGG + chr8.3:14715459-14715478 MS.gene038800:CDS 50.0%
GAAGGAGAGGAAGAAGATGC+AGG + chr8.3:14715428-14715447 MS.gene038800:CDS 50.0%
TTCTTGGGGCCTGTTTGGAT+TGG - chr8.3:14716000-14716019 None:intergenic 50.0%
GAAATCGGTGCAGAGGGAGA+AGG + chr8.3:14715410-14715429 MS.gene038800:CDS 55.0%
CAACGGCAGGAGCAACGAAG+AGG + chr8.3:14717296-14717315 MS.gene038800:CDS 60.0%
TCGTTGCTCCTGCCGTTGCA+AGG - chr8.3:14717294-14717313 None:intergenic 60.0%
CGGTGCAGAGGGAGAAGGAG+AGG + chr8.3:14715415-14715434 MS.gene038800:CDS 65.0%
CTCCTGCCGTTGCAAGGTCC+TGG - chr8.3:14717288-14717307 None:intergenic 65.0%
GTCCAGGACCTTGCAACGGC+AGG + chr8.3:14717283-14717302 MS.gene038800:intron 65.0%


Chromosome Type Strat End Strand Name
chr8.3 gene 14715387 14717450 14715387 ID=MS.gene038800
chr8.3 mRNA 14715387 14717450 14715387 ID=MS.gene038800.t1;Parent=MS.gene038800
chr8.3 exon 14715387 14715522 14715387 ID=MS.gene038800.t1.exon1;Parent=MS.gene038800.t1
chr8.3 CDS 14715387 14715522 14715387 ID=cds.MS.gene038800.t1;Parent=MS.gene038800.t1
chr8.3 exon 14716249 14716318 14716249 ID=MS.gene038800.t1.exon2;Parent=MS.gene038800.t1
chr8.3 CDS 14716249 14716318 14716249 ID=cds.MS.gene038800.t1;Parent=MS.gene038800.t1
chr8.3 exon 14716502 14716607 14716502 ID=MS.gene038800.t1.exon3;Parent=MS.gene038800.t1
chr8.3 CDS 14716502 14716607 14716502 ID=cds.MS.gene038800.t1;Parent=MS.gene038800.t1
chr8.3 exon 14717289 14717450 14717289 ID=MS.gene038800.t1.exon4;Parent=MS.gene038800.t1
chr8.3 CDS 14717289 14717450 14717289 ID=cds.MS.gene038800.t1;Parent=MS.gene038800.t1
Gene Sequence

>MS.gene038800

ATGGGAAGCGACAGTGAAGGTGAGAAATCGGTGCAGAGGGAGAAGGAGAGGAAGAAGATGCAGGCTCTAGCCCCCATTGCAAAACCTCTTGCTGGAAAGAAGCTTTCCAAGAAAACACTCAAGCTTGTTAAAAGAGGTATTTTCGAAGCTTTTAAACACTATAGCTTGTTGAATTAATGAAAATCGTTAATTTGTGGCTTCTAAGAATTGCAGTGTTTCTGGGTTTTTGGAGTTAGTAAGAAAAATCACAACTTCTCTTTAATTTCAAAATGTATAATGCATCAAGTTTCTTATGTATGCTATGTAATTAGAGTATAAACTTCAAAAATGAACTTGTTATGGAATTAAGGGCCTGTTTGGATAAACAACTTATTTACAGCTTATAGCTCAAGCGCTTATCACACTTATGTATAAGCTTGCATAAGCTATGTTTATAGCAAAAGATTAAATTAACGTTAATTTGTTTTTGTATATGCCTAAGTTGTTTTTATAAGCTATAAACTATATTGGAGAGTTCTTGAAAATAAGTGGACAAAAGCTTATGAAAATTGTTATCAGCTGTTTTTACAAATTCTCACAAAATGTATGCCAGTAGATCAGCTGAAATAAGCCAATCCAAACAGGCCCCAAGAATGTTATGTTATGAATCTAAGTATTATATTTTGCTGTCTGCTGTTACCTTTTTGCTGTCTTTTTACCATACAAATTTGCTGCCGTTTTTTCTTTTATTTTGGAATGGCTAATGTTAATTTGTTGATTCTGTAATTTAAAAAGGAAAAAAATTAGCTTGAATGTTTTGTCAATTTGGATTACATAAATCTTTGTCTTATGGGACTATACTAATCTAGTGTGTTCTTTTCAGCTGCTGAAAACAAGTGCATAAAGAGAGGAGTTAAGGAGGTGGTTAAAAGTATAAGGCGAGGTCAAAAAGGGTCAGTATTAAGCTAAACATTAGATTGCTCACAAAGGGCATTAGAAATAATTAATTAATTCAAACTATGTTACTATCATATCTTCCTTGACAAAATGGTTACTCAATGTGAATTCCCATTTTTTATGTTTCAATTGTGGAAAATAAATGTGTACCCTTATTTTTTGCCTTCTTCTCAATGCAGAGTGTGTATTATAGCTGGGAACATCTCGCCTATTGATGTCATCACTCATGTTCCGATTTTATGCGAGGATAAGGACATTCCATATGTGTATGTCTCATCTAAAGAAGTAAGTAACCAACTTTGCGCTGAATCTTGATGGTTTTTATCATTTAGTTAATACTATTGACCGCCTGTTTCCCTAATCTCTCTTTAGAATAAGAAAAATTTGTTGTCATATATTTTCTTACACATATAGCCTGAGGTTAATTTGATGTCATAAATAACCAAAAGTTGAGGCTTTTTCCTCATTGATGATGTTGCACCTTGTTTTGTAACTTGAGTGCTTTTCCTTATGCTGGCATTCATAACACAAATTGTGTTGACAGGAGTTGAGTCCATGTAACATATTTGAATGGAATGTATGCTTTTCTATACTGGATTGAATCATTTAGAGTATATCATACTTCAAGAAACATCTTTCAGCTATTTTAATTTTTTTATTGACGCATCTGTCATCTATAATCCAGCTCATAGACCTGTATATTATGAATACAATAGTCATTGCACAGTTTATCGAGAAAGAAAGGATAATTTTTTGTTTCTCTTACCTACATAATATATCAACAATTCAACATGTGATATTTTCAGTTTCGTTTTAAATATGGTATTAAGAGAAACATGGTTGAAATTGCTATTCTTCAAAAAAATCTGCTATGCAATCATTTGGTGTTCATATATATGTTAAGAAACTCCAATTACTCTAGTACTTTGAAAGTTCATATATTAAATTTCTGAGATATTTGTCCAGGACCTTGCAACGGCAGGAGCAACGAAGAGGCCAACTTGTTGTGTTTTAGTGATGACCAAGCCTTCAAAGGGTGAACTATCTCAAGAGGTACAAGAGAAACTAAAGTCAGAGTATGATCAAGTTGCATCAGAGTGTAGAGAGCTTCAGTCTACACTTTTTTGA

Protein sequence

>MS.gene038800.t1

MGSDSEGEKSVQREKERKKMQALAPIAKPLAGKKLSKKTLKLVKRAAENKCIKRGVKEVVKSIRRGQKGVCIIAGNISPIDVITHVPILCEDKDIPYVYVSSKEDLATAGATKRPTCCVLVMTKPSKGELSQEVQEKLKSEYDQVASECRELQSTLF