AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene033017


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene033017.t1 MTR_4g112780 97.452 157 0 1 1 157 1 153 1.26e-106 301
MS.gene033017.t1 MTR_4g114330 34.483 116 75 1 21 136 4 118 2.17e-14 66.2
MS.gene033017.t1 MTR_5g077600 72.727 44 11 1 105 148 23 65 6.86e-14 63.2
MS.gene033017.t1 MTR_0011s0330 34.821 112 72 1 25 136 8 118 2.09e-13 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene033017.t1 AT5G08180 68.153 157 49 1 1 157 1 156 4.91e-76 224
MS.gene033017.t1 AT5G08180 68.153 157 49 1 1 157 1 156 4.91e-76 224
MS.gene033017.t1 AT4G12600 34.188 117 76 1 20 136 3 118 5.16e-13 62.4
MS.gene033017.t1 AT5G20160 35.714 112 71 1 25 136 8 118 5.27e-13 62.4
MS.gene033017.t1 AT4G22380 34.821 112 72 1 25 136 8 118 9.05e-13 62.0

Find 32 sgRNAs with CRISPR-Local

Find 107 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGTATAAGGCGAGGTCAAAA+AGG 0.296737 8.4:+17507550 MS.gene033017:CDS
CCCATTGCAAAACCTCTTGC+TGG 0.348880 8.4:+17506719 MS.gene033017:CDS
TGAGATAGTTCACCCTTTGA+AGG 0.372203 8.4:-17508612 None:intergenic
AGGAGGTGGTTAAAAGTATA+AGG 0.394940 8.4:+17507536 MS.gene033017:CDS
GTGCATAAAGAGAGGAGTTA+AGG 0.402782 8.4:+17507516 MS.gene033017:CDS
TCCTTATCCTCGCATAAAAT+CGG 0.425786 8.4:-17507816 None:intergenic
TGCAGAGTGTGTATTATAGC+TGG 0.425932 8.4:+17507759 MS.gene033017:intron
TAGTTCACCCTTTGAAGGCT+TGG 0.444404 8.4:-17508607 None:intergenic
ACATGAGTGATGACATCAAT+AGG 0.449731 8.4:-17507792 None:intergenic
TTAGATGAGACATATACATA+TGG 0.460404 8.4:-17507843 None:intergenic
GTCCAGGACCTTGCAACGGC+AGG 0.468819 8.4:+17508546 MS.gene033017:intron
CGACAGTGAAGGTGAGAAAT+CGG 0.483487 8.4:+17506655 MS.gene033017:CDS
GTGATGACCAAGCCTTCAAA+GGG 0.500756 8.4:+17508600 MS.gene033017:CDS
TCGTTGCTCCTGCCGTTGCA+AGG 0.501816 8.4:-17508554 None:intergenic
AGTGATGACCAAGCCTTCAA+AGG 0.525648 8.4:+17508599 MS.gene033017:CDS
ACACTCAAGCTTGTTAAAAG+AGG 0.527156 8.4:+17506761 MS.gene033017:CDS
AAAGAGAGGAGTTAAGGAGG+TGG 0.543156 8.4:+17507522 MS.gene033017:CDS
CGGTGCAGAGGGAGAAGGAG+AGG 0.549279 8.4:+17506675 MS.gene033017:CDS
CATAAAGAGAGGAGTTAAGG+AGG 0.557458 8.4:+17507519 MS.gene033017:CDS
GAAAGCTTCTTTCCAGCAAG+AGG 0.560382 8.4:-17506731 None:intergenic
GCAGAGTGTGTATTATAGCT+GGG 0.568819 8.4:+17507760 MS.gene033017:intron
ATCACTAAAACACAACAAGT+TGG 0.571248 8.4:-17508582 None:intergenic
GAAGGAGAGGAAGAAGATGC+AGG 0.571568 8.4:+17506688 MS.gene033017:CDS
GAAATCGGTGCAGAGGGAGA+AGG 0.585599 8.4:+17506670 MS.gene033017:CDS
AAGGTGAGAAATCGGTGCAG+AGG 0.589190 8.4:+17506663 MS.gene033017:CDS
AGGTGAGAAATCGGTGCAGA+GGG 0.594395 8.4:+17506664 MS.gene033017:CDS
AAAGGGTGAACTATCTCAAG+AGG 0.620179 8.4:+17508617 MS.gene033017:CDS
GAAAACAAGTGCATAAAGAG+AGG 0.625540 8.4:+17507508 MS.gene033017:CDS
ATTTGTCCAGGACCTTGCAA+CGG 0.636321 8.4:+17508542 MS.gene033017:intron
CAACGGCAGGAGCAACGAAG+AGG 0.638950 8.4:+17508559 MS.gene033017:CDS
GAAATGGGAAGCGACAGTGA+AGG 0.681973 8.4:+17506644 None:intergenic
GTGGTTAAAAGTATAAGGCG+AGG 0.693539 8.4:+17507541 MS.gene033017:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ACAATTGAAACATAAAAAAT+GGG - chr8.4:17507697-17507716 None:intergenic 15.0%
!! TTATAAGCTATAAACTATAT+TGG + chr8.4:17507115-17507134 MS.gene033017:intron 15.0%
!! TTCCAAAAAAAAAGAAAAAA+CGG - chr8.4:17507356-17507375 None:intergenic 15.0%
!!! TTTTTTCTTTTTTTTTGGAA+TGG + chr8.4:17507356-17507375 MS.gene033017:intron 15.0%
!! ATAGCTTATAAAAACAACTT+AGG - chr8.4:17507106-17507125 None:intergenic 20.0%
!! CACAATTGAAACATAAAAAA+TGG - chr8.4:17507698-17507717 None:intergenic 20.0%
!! CTATTGTATTCATAATATAC+AGG - chr8.4:17508281-17508300 None:intergenic 20.0%
!! TACATAAATCTTTGTCTTAT+GGG + chr8.4:17507450-17507469 MS.gene033017:intron 20.0%
!! TCATAAATAACTAAAAGTTG+AGG + chr8.4:17508017-17508036 MS.gene033017:intron 20.0%
!! TTACATAAATCTTTGTCTTA+TGG + chr8.4:17507449-17507468 MS.gene033017:intron 20.0%
!! TTCCATTCAAATATGTTATA+TGG - chr8.4:17508141-17508160 None:intergenic 20.0%
!!! CATTTTTTATGTTTCAATTG+TGG + chr8.4:17507696-17507715 MS.gene033017:intron 20.0%
!!! GTTGATTCTGTAATTTAAAA+AGG + chr8.4:17507392-17507411 MS.gene033017:intron 20.0%
!!! TAATGAAAATCGTTAATTTG+TGG + chr8.4:17506821-17506840 MS.gene033017:intron 20.0%
!!! TTTCAGTTTCGTTTTAAATA+TGG + chr8.4:17508385-17508404 MS.gene033017:intron 20.0%
! AATAAGTTGTTTATCCAAAC+AGG - chr8.4:17507008-17507027 None:intergenic 25.0%
! AGAAGAAGGCAAAAAAATAA+GGG - chr8.4:17507736-17507755 None:intergenic 25.0%
! ATATGGTATTAAGAGAAACA+TGG + chr8.4:17508402-17508421 MS.gene033017:intron 25.0%
! ATTCATAACATACCATTCTT+GGG - chr8.4:17507268-17507287 None:intergenic 25.0%
! ATTTATCTTCCTTGACAAAA+TGG + chr8.4:17507649-17507668 MS.gene033017:intron 25.0%
! GAATTGTTGATATACTATGT+AGG - chr8.4:17508353-17508372 None:intergenic 25.0%
! GTCCATATAACATATTTGAA+TGG + chr8.4:17508136-17508155 MS.gene033017:intron 25.0%
! TTAGATGAGACATATACATA+TGG - chr8.4:17507846-17507865 None:intergenic 25.0%
!! CTTATTCTAAAGAGAGATTA+GGG - chr8.4:17507943-17507962 None:intergenic 25.0%
!! TCTTATTCTAAAGAGAGATT+AGG - chr8.4:17507944-17507963 None:intergenic 25.0%
!! TTTCAAAGTACTAGAGTAAT+TGG - chr8.4:17508498-17508517 None:intergenic 25.0%
!!! AATTTTTGAGATATTTGTCC+AGG + chr8.4:17508530-17508549 MS.gene033017:intron 25.0%
!!! ATCAACTTGTTATGGAATTA+AGG + chr8.4:17506981-17507000 MS.gene033017:intron 25.0%
!!! CTTCAAAAATCAACTTGTTA+TGG + chr8.4:17506973-17506992 MS.gene033017:intron 25.0%
!!! TCAACTTGTTATGGAATTAA+GGG + chr8.4:17506982-17507001 MS.gene033017:intron 25.0%
!!! TGCCGTTTTTTCTTTTTTTT+TGG + chr8.4:17507351-17507370 MS.gene033017:intron 25.0%
AAATCTGCTATGCAATCATT+TGG + chr8.4:17508448-17508467 MS.gene033017:intron 30.0%
AACATTAGATTGCTCACAAA+GGG + chr8.4:17507587-17507606 MS.gene033017:intron 30.0%
ACAATTTGTGTTATGAATGC+TGG - chr8.4:17508101-17508120 None:intergenic 30.0%
ATCACTAAAACACAACAAGT+TGG - chr8.4:17508585-17508604 None:intergenic 30.0%
ATGGTAAAAAGACAGCAAAA+AGG - chr8.4:17507321-17507340 None:intergenic 30.0%
GAGAAGAAGGCAAAAAAATA+AGG - chr8.4:17507737-17507756 None:intergenic 30.0%
GATTCATAACATACCATTCT+TGG - chr8.4:17507269-17507288 None:intergenic 30.0%
GGAGAATTCTTCAAAATAAG+TGG + chr8.4:17507136-17507155 MS.gene033017:intron 30.0%
TAACATTAGATTGCTCACAA+AGG + chr8.4:17507586-17507605 MS.gene033017:intron 30.0%
TATGACATCAAATTAACCTC+AGG - chr8.4:17508002-17508021 None:intergenic 30.0%
TCTATGAGCTGAATTATAGA+TGG - chr8.4:17508258-17508277 None:intergenic 30.0%
TTCATAACATACCATTCTTG+GGG - chr8.4:17507267-17507286 None:intergenic 30.0%
! ATAACACAAATTGTGTTGAC+AGG + chr8.4:17508107-17508126 MS.gene033017:intron 30.0%
! GAATGTATGCTTTTCTATAC+TGG + chr8.4:17508158-17508177 MS.gene033017:intron 30.0%
! GCTTGAATGTTTTGTCAATT+TGG + chr8.4:17507425-17507444 MS.gene033017:intron 30.0%
! TACATTTTGTGAGTGTGTTT+TGG - chr8.4:17507206-17507225 None:intergenic 30.0%
AAAACGGCAGCAAATTTGTA+TGG - chr8.4:17507340-17507359 None:intergenic 35.0%
ACATGAGTGATGACATCAAT+AGG - chr8.4:17507795-17507814 None:intergenic 35.0%
AGCACTCAAGTTACAAAACA+AGG - chr8.4:17508068-17508087 None:intergenic 35.0%
CACAGTTTATCGAGAAAGAA+AGG + chr8.4:17508307-17508326 MS.gene033017:intron 35.0%
CTTGACAAAATGGTTACTCA+AGG + chr8.4:17507659-17507678 MS.gene033017:intron 35.0%
GAAAACAAGTGCATAAAGAG+AGG + chr8.4:17507508-17507527 MS.gene033017:CDS 35.0%
TAAAGAGAGATTAGGGAAAC+AGG - chr8.4:17507936-17507955 None:intergenic 35.0%
TCCTTATCCTCGCATAAAAT+CGG - chr8.4:17507819-17507838 None:intergenic 35.0%
TGAAATAAGCCAATCCAAAC+AGG + chr8.4:17507241-17507260 MS.gene033017:intron 35.0%
TTTCTTACACATATAGCCTG+AGG + chr8.4:17507983-17508002 MS.gene033017:intron 35.0%
! AACAAGCTTGAGTGTTTTCT+TGG - chr8.4:17506756-17506775 None:intergenic 35.0%
! AGGAGGTGGTTAAAAGTATA+AGG + chr8.4:17507536-17507555 MS.gene033017:CDS 35.0%
! CTTGAGTAACCATTTTGTCA+AGG - chr8.4:17507661-17507680 None:intergenic 35.0%
!! ACACTCAAGCTTGTTAAAAG+AGG + chr8.4:17506761-17506780 MS.gene033017:CDS 35.0%
AAAGGGTGAACTATCTCAAG+AGG + chr8.4:17508617-17508636 MS.gene033017:CDS 40.0%
AACTTTGCGCTGAATCTTGA+TGG + chr8.4:17507880-17507899 MS.gene033017:intron 40.0%
AAGGTGCAACATCATCAATG+AGG - chr8.4:17508049-17508068 None:intergenic 40.0%
AGTATAAGGCGAGGTCAAAA+AGG + chr8.4:17507550-17507569 MS.gene033017:CDS 40.0%
ATCAAGATTCAGCGCAAAGT+TGG - chr8.4:17507881-17507900 None:intergenic 40.0%
CATAAAGAGAGGAGTTAAGG+AGG + chr8.4:17507519-17507538 MS.gene033017:CDS 40.0%
GCAGAGTGTGTATTATAGCT+GGG + chr8.4:17507760-17507779 MS.gene033017:intron 40.0%
GCTTATTTCAGCTGATCTAC+TGG - chr8.4:17507231-17507250 None:intergenic 40.0%
GTATAAGGCGAGGTCAAAAA+GGG + chr8.4:17507551-17507570 MS.gene033017:CDS 40.0%
GTGCATAAAGAGAGGAGTTA+AGG + chr8.4:17507516-17507535 MS.gene033017:CDS 40.0%
GTGTTATGAATGCTGGCATA+AGG - chr8.4:17508094-17508113 None:intergenic 40.0%
TATGGAATTAAGGGCCTGTT+TGG + chr8.4:17506991-17507010 MS.gene033017:intron 40.0%
TGAGATAGTTCACCCTTTGA+AGG - chr8.4:17508615-17508634 None:intergenic 40.0%
TGCAGAGTGTGTATTATAGC+TGG + chr8.4:17507759-17507778 MS.gene033017:intron 40.0%
! CTAAGCATTGCAGTGTTTCT+GGG + chr8.4:17506847-17506866 MS.gene033017:intron 40.0%
! GTGGTTAAAAGTATAAGGCG+AGG + chr8.4:17507541-17507560 MS.gene033017:CDS 40.0%
! TCATGTTCCGATTTTATGCG+AGG + chr8.4:17507809-17507828 MS.gene033017:CDS 40.0%
! TCCGATTTTATGCGAGGATA+AGG + chr8.4:17507815-17507834 MS.gene033017:CDS 40.0%
! TCTAAGCATTGCAGTGTTTC+TGG + chr8.4:17506846-17506865 MS.gene033017:intron 40.0%
!!! TTGCAGTGTTTCTGGGTTTT+TGG + chr8.4:17506854-17506873 MS.gene033017:intron 40.0%
AAAGAGAGGAGTTAAGGAGG+TGG + chr8.4:17507522-17507541 MS.gene033017:CDS 45.0%
AGAGAGATTAGGGAAACAGG+CGG - chr8.4:17507933-17507952 None:intergenic 45.0%
AGTGATGACCAAGCCTTCAA+AGG + chr8.4:17508599-17508618 MS.gene033017:CDS 45.0%
ATTTGTCCAGGACCTTGCAA+CGG + chr8.4:17508542-17508561 MS.gene033017:intron 45.0%
CACACTCTGCATTGAGAAGA+AGG - chr8.4:17507750-17507769 None:intergenic 45.0%
CGACAGTGAAGGTGAGAAAT+CGG + chr8.4:17506655-17506674 MS.gene033017:CDS 45.0%
GAAAGCTTCTTTCCAGCAAG+AGG - chr8.4:17506734-17506753 None:intergenic 45.0%
GTGATGACCAAGCCTTCAAA+GGG + chr8.4:17508600-17508619 MS.gene033017:CDS 45.0%
! AGCAAGAGGTTTTGCAATGG+GGG - chr8.4:17506720-17506739 None:intergenic 45.0%
! CAGCAAGAGGTTTTGCAATG+GGG - chr8.4:17506721-17506740 None:intergenic 45.0%
! CCAGCAAGAGGTTTTGCAAT+GGG - chr8.4:17506722-17506741 None:intergenic 45.0%
! TAGTTCACCCTTTGAAGGCT+TGG - chr8.4:17508610-17508629 None:intergenic 45.0%
! TCCAGCAAGAGGTTTTGCAA+TGG - chr8.4:17506723-17506742 None:intergenic 45.0%
AAGGTGAGAAATCGGTGCAG+AGG + chr8.4:17506663-17506682 MS.gene033017:CDS 50.0%
AGGTGAGAAATCGGTGCAGA+GGG + chr8.4:17506664-17506683 MS.gene033017:CDS 50.0%
ATCCAAACAGGCCCCAAGAA+TGG + chr8.4:17507253-17507272 MS.gene033017:intron 50.0%
CCCATTGCAAAACCTCTTGC+TGG + chr8.4:17506719-17506738 MS.gene033017:CDS 50.0%
GAAGGAGAGGAAGAAGATGC+AGG + chr8.4:17506688-17506707 MS.gene033017:CDS 50.0%
TACCATTCTTGGGGCCTGTT+TGG - chr8.4:17507258-17507277 None:intergenic 50.0%
TTCTTGGGGCCTGTTTGGAT+TGG - chr8.4:17507253-17507272 None:intergenic 50.0%
GAAATCGGTGCAGAGGGAGA+AGG + chr8.4:17506670-17506689 MS.gene033017:CDS 55.0%
CAACGGCAGGAGCAACGAAG+AGG + chr8.4:17508559-17508578 MS.gene033017:CDS 60.0%
TCGTTGCTCCTGCCGTTGCA+AGG - chr8.4:17508557-17508576 None:intergenic 60.0%
CGGTGCAGAGGGAGAAGGAG+AGG + chr8.4:17506675-17506694 MS.gene033017:CDS 65.0%
CTCCTGCCGTTGCAAGGTCC+TGG - chr8.4:17508551-17508570 None:intergenic 65.0%
GTCCAGGACCTTGCAACGGC+AGG + chr8.4:17508546-17508565 MS.gene033017:intron 65.0%


Chromosome Type Strat End Strand Name
chr8.4 gene 17506647 17508713 17506647 ID=MS.gene033017
chr8.4 mRNA 17506647 17508713 17506647 ID=MS.gene033017.t1;Parent=MS.gene033017
chr8.4 exon 17506647 17506782 17506647 ID=MS.gene033017.t1.exon1;Parent=MS.gene033017.t1
chr8.4 CDS 17506647 17506782 17506647 ID=cds.MS.gene033017.t1;Parent=MS.gene033017.t1
chr8.4 exon 17507503 17507572 17507503 ID=MS.gene033017.t1.exon2;Parent=MS.gene033017.t1
chr8.4 CDS 17507503 17507572 17507503 ID=cds.MS.gene033017.t1;Parent=MS.gene033017.t1
chr8.4 exon 17507764 17507869 17507764 ID=MS.gene033017.t1.exon3;Parent=MS.gene033017.t1
chr8.4 CDS 17507764 17507869 17507764 ID=cds.MS.gene033017.t1;Parent=MS.gene033017.t1
chr8.4 exon 17508552 17508713 17508552 ID=MS.gene033017.t1.exon4;Parent=MS.gene033017.t1
chr8.4 CDS 17508552 17508713 17508552 ID=cds.MS.gene033017.t1;Parent=MS.gene033017.t1
Gene Sequence

>MS.gene033017

ATGGGAAGCGACAGTGAAGGTGAGAAATCGGTGCAGAGGGAGAAGGAGAGGAAGAAGATGCAGGCTCTAGCCCCCATTGCAAAACCTCTTGCTGGAAAGAAGCTTTCCAAGAAAACACTCAAGCTTGTTAAAAGAGGTATTTTCGAAGCTTTTAAACACTATAGCTTGTTGAATTAATGAAAATCGTTAATTTGTGGCTTCTAAGCATTGCAGTGTTTCTGGGTTTTTGGAGTTAGTAAGAAAAATCACAACTTTTCTTTAATTTCAAAATTTAACATGTATAATGCATCGAGTTTCTTATGTATGTTATGTAATTAGAGTATAAACTTCAAAAATCAACTTGTTATGGAATTAAGGGCCTGTTTGGATAAACAACTTATTTACAGCTTGTATAAGCTTGCATATGCTATGTTTATAGCAAAAGATTAAATTAACGTTAATTTGTTTTTGTATATGCCTAAGTTGTTTTTATAAGCTATAAACTATATTGGAGAATTCTTCAAAATAAGTGGACAAAAGCTTATGAAAATTGTTATCAGCTGTTTTTACAAATTCTCCAAAACACACTCACAAAATGTATGCCAGTAGATCAGCTGAAATAAGCCAATCCAAACAGGCCCCAAGAATGGTATGTTATGAATCTAAGTATTATATTTTGCAGTCTGCTGTTACCTTTTTGCTGTCTTTTTACCATACAAATTTGCTGCCGTTTTTTCTTTTTTTTTGGAATGGCTAATGTTAATTTGTTGATTCTGTAATTTAAAAAGGAAAAAAATTAGCTTGAATGTTTTGTCAATTTGGATTACATAAATCTTTGTCTTATGGGACTATACTAATCTATGTGTGTTCTTTTCAGCTGCTGAAAACAAGTGCATAAAGAGAGGAGTTAAGGAGGTGGTTAAAAGTATAAGGCGAGGTCAAAAAGGGTCAGTATTAAGCTAACATTAGATTGCTCACAAAGGGCATTAGAAATAATTAATTAATTCAAACTATGTTACTATCATTTATCTTCCTTGACAAAATGGTTACTCAAGGTGTATGTGAATTCCCATTTTTTATGTTTCAATTGTGGAAAATAAATGTGTACCCTTATTTTTTTGCCTTCTTCTCAATGCAGAGTGTGTATTATAGCTGGGAACATATCGCCTATTGATGTCATCACTCATGTTCCGATTTTATGCGAGGATAAGGACATTCCATATGTATATGTCTCATCTAAAGAAGTAAGTAACCAACTTTGCGCTGAATCTTGATGGTTTTTATCATTTAGTTAATACTATTGACCGCCTGTTTCCCTAATCTCTCTTTAGAATAAGAAAAATTTGTTGTCATATATTTTCTTACACATATAGCCTGAGGTTAATTTGATGTCATAAATAACTAAAAGTTGAGGCTTTTTCCTCATTGATGATGTTGCACCTTGTTTTGTAACTTGAGTGCTTTTCCTTATGCCAGCATTCATAACACAAATTGTGTTGACAGGAGTTGAGTCCATATAACATATTTGAATGGAATGTATGCTTTTCTATACTGGATTGAATCATTTAGAGTATATCATACTTCAAGAAACATCTTTCAGCTATTTTAATTTTTTTATAGACGCATCTGCCATCTATAATTCAGCTCATAGACCTGTATATTATGAATACAATAGTCATTGCACAGTTTATCGAGAAAGAAAGGATAATTTTTTGCTTCTCTTACCTACATAGTATATCAACAATTCAACATGTGATGTTTTCAGTTTCGTTTTAAATATGGTATTAAGAGAAACATGGTTGAAATTGCTATTTTTCAAAAAAAATCTGCTATGCAATCATTTGGTGTTCATATATATGTTAAGAAACTCCAATTACTCTAGTACTTTGAAAGTTCATATATTAAATTTTTGAGATATTTGTCCAGGACCTTGCAACGGCAGGAGCAACGAAGAGGCCAACTTGTTGTGTTTTAGTGATGACCAAGCCTTCAAAGGGTGAACTATCTCAAGAGGTACAAGAGAAACTAAAGTCAGAGTATGATCAAGTTGCATCAGAGTGTAGAGAGCTTCAGTCTACACTTTTTTGA

Protein sequence

>MS.gene033017.t1

MGSDSEGEKSVQREKERKKMQALAPIAKPLAGKKLSKKTLKLVKRAAENKCIKRGVKEVVKSIRRGQKGVCIIAGNISPIDVITHVPILCEDKDIPYVYVSSKEDLATAGATKRPTCCVLVMTKPSKGELSQEVQEKLKSEYDQVASECRELQSTLF