AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene68979


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene68979.t1 MTR_4g112780 97.452 157 0 1 1 157 1 153 1.26e-106 301
MS.gene68979.t1 MTR_4g114330 34.483 116 75 1 21 136 4 118 2.17e-14 66.2
MS.gene68979.t1 MTR_5g077600 72.727 44 11 1 105 148 23 65 6.86e-14 63.2
MS.gene68979.t1 MTR_0011s0330 34.821 112 72 1 25 136 8 118 2.09e-13 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene68979.t1 AT5G08180 68.153 157 49 1 1 157 1 156 4.91e-76 224
MS.gene68979.t1 AT5G08180 68.153 157 49 1 1 157 1 156 4.91e-76 224
MS.gene68979.t1 AT4G12600 34.188 117 76 1 20 136 3 118 5.16e-13 62.4
MS.gene68979.t1 AT5G20160 35.714 112 71 1 25 136 8 118 5.27e-13 62.4
MS.gene68979.t1 AT4G22380 34.821 112 72 1 25 136 8 118 9.05e-13 62.0

Find 31 sgRNAs with CRISPR-Local

Find 105 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGTATAAGGCGAGGTCAAAA+AGG 0.296737 8.1:+15284755 MS.gene68979:CDS
CCCATTGCAAAACCTCTTGC+TGG 0.348880 8.1:+15283905 MS.gene68979:CDS
TGAGATAGTTCACCCTTTGA+AGG 0.372203 8.1:-15285821 None:intergenic
CGCAGAGTGTGTATTATAGC+TGG 0.392404 8.1:+15284968 MS.gene68979:intron
AGGAGGTGGTTAAAAGTATA+AGG 0.394940 8.1:+15284741 MS.gene68979:CDS
GTGCATAAAGAGAGGAGTTA+AGG 0.402782 8.1:+15284721 MS.gene68979:CDS
TAGTTCACCCTTTGAAGGCT+TGG 0.444404 8.1:-15285816 None:intergenic
ACATGAGTGATGACATCAAT+AGG 0.449731 8.1:-15285001 None:intergenic
TCCTTATCCTCACATAAAAT+CGG 0.452854 8.1:-15285025 None:intergenic
TTAGATGAGACATATACATA+TGG 0.460404 8.1:-15285052 None:intergenic
GTCCAGGACCTTGCAACGGC+AGG 0.468819 8.1:+15285755 MS.gene68979:intron
CGACAGTGAAGGTGAGAAAT+CGG 0.483487 8.1:+15283841 MS.gene68979:CDS
GTGATGACCAAGCCTTCAAA+GGG 0.500756 8.1:+15285809 MS.gene68979:CDS
TCGTTGCTCCTGCCGTTGCA+AGG 0.501816 8.1:-15285763 None:intergenic
AGTGATGACCAAGCCTTCAA+AGG 0.525648 8.1:+15285808 MS.gene68979:CDS
ACACTCAAGCTTGTTAAAAG+AGG 0.527156 8.1:+15283947 MS.gene68979:CDS
AAAGAGAGGAGTTAAGGAGG+TGG 0.543156 8.1:+15284727 MS.gene68979:CDS
CGGTGCAGAGGGAGAAGGAG+AGG 0.549279 8.1:+15283861 MS.gene68979:CDS
CATAAAGAGAGGAGTTAAGG+AGG 0.557458 8.1:+15284724 MS.gene68979:CDS
GAAAGCTTCTTTCCAGCAAG+AGG 0.560382 8.1:-15283917 None:intergenic
ATCACTAAAACACAACAAGT+TGG 0.561953 8.1:-15285791 None:intergenic
GAAGGAGAGGAAGAAGATGC+AGG 0.571568 8.1:+15283874 MS.gene68979:CDS
GAAATCGGTGCAGAGGGAGA+AGG 0.585599 8.1:+15283856 MS.gene68979:CDS
AAGGTGAGAAATCGGTGCAG+AGG 0.589190 8.1:+15283849 MS.gene68979:CDS
GCAGAGTGTGTATTATAGCT+GGG 0.592201 8.1:+15284969 MS.gene68979:intron
AGGTGAGAAATCGGTGCAGA+GGG 0.594395 8.1:+15283850 MS.gene68979:CDS
AAAGGGTGAACTATCTCAAG+AGG 0.620179 8.1:+15285826 MS.gene68979:CDS
GAAAACAAGTGCATAAAGAG+AGG 0.625540 8.1:+15284713 MS.gene68979:CDS
ATTTGTCCAGGACCTTGCAA+CGG 0.636321 8.1:+15285751 MS.gene68979:intron
GAAATGGGAAGCGACAGTGA+AGG 0.681973 8.1:+15283830 None:intergenic
GTGGTTAAAAGTATAAGGCG+AGG 0.693539 8.1:+15284746 MS.gene68979:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ACAATTGAAACATAAAAAAT+GGG - chr8.1:15284907-15284926 None:intergenic 15.0%
!! ATAGCTTATAAAAACAATTT+AGG - chr8.1:15284311-15284330 None:intergenic 15.0%
!! TTATAAGCTATAAACTATAT+TGG + chr8.1:15284320-15284339 MS.gene68979:intron 15.0%
!! TTCCAAAAAAAAAGAAAAAA+CGG - chr8.1:15284561-15284580 None:intergenic 15.0%
!!! TTTTTTCTTTTTTTTTGGAA+TGG + chr8.1:15284561-15284580 MS.gene68979:intron 15.0%
!! ATTCATAACATAACATTCTT+GGG - chr8.1:15284473-15284492 None:intergenic 20.0%
!! CACAATTGAAACATAAAAAA+TGG - chr8.1:15284908-15284927 None:intergenic 20.0%
!! CTATTGTATTCATAATATAC+AGG - chr8.1:15285490-15285509 None:intergenic 20.0%
!! GAATTGTTGATATATTATGT+AGG - chr8.1:15285562-15285581 None:intergenic 20.0%
!! TACATAAATCTTTGTCTTAT+GGG + chr8.1:15284655-15284674 MS.gene68979:intron 20.0%
!! TCATAAATAACTAAAAGTTG+AGG + chr8.1:15285226-15285245 MS.gene68979:intron 20.0%
!! TTACATAAATCTTTGTCTTA+TGG + chr8.1:15284654-15284673 MS.gene68979:intron 20.0%
!! TTCCATTCAAATATGTTATA+TGG - chr8.1:15285350-15285369 None:intergenic 20.0%
!!! CATTTTTTATGTTTCAATTG+TGG + chr8.1:15284906-15284925 MS.gene68979:intron 20.0%
!!! GTTGATTCTGTAATTTAAAA+AGG + chr8.1:15284597-15284616 MS.gene68979:intron 20.0%
!!! TAATGAAAATCGTTAATTTG+TGG + chr8.1:15284007-15284026 MS.gene68979:intron 20.0%
!!! TTTCAGTTTCGTTTTAAATA+TGG + chr8.1:15285594-15285613 MS.gene68979:intron 20.0%
! AATAAGTTGTTTATCCAAAC+AGG - chr8.1:15284187-15284206 None:intergenic 25.0%
! ATATGGTATTAAGAGAAACA+TGG + chr8.1:15285611-15285630 MS.gene68979:intron 25.0%
! ATTTATCTTCCTTGACAAAA+TGG + chr8.1:15284854-15284873 MS.gene68979:intron 25.0%
! GATTCATAACATAACATTCT+TGG - chr8.1:15284474-15284493 None:intergenic 25.0%
! GTCCATATAACATATTTGAA+TGG + chr8.1:15285345-15285364 MS.gene68979:intron 25.0%
! TTAGATGAGACATATACATA+TGG - chr8.1:15285055-15285074 None:intergenic 25.0%
! TTCATAACATAACATTCTTG+GGG - chr8.1:15284472-15284491 None:intergenic 25.0%
!! CTTATTCTAAAGAGAGATTA+GGG - chr8.1:15285152-15285171 None:intergenic 25.0%
!! TACATTTTGTAAGTCTGTTT+TGG - chr8.1:15284411-15284430 None:intergenic 25.0%
!! TCTTATTCTAAAGAGAGATT+AGG - chr8.1:15285153-15285172 None:intergenic 25.0%
!! TTTCAAAGTACTAGAGTAAT+TGG - chr8.1:15285707-15285726 None:intergenic 25.0%
!!! AATTTTTGAGATATTTGTCC+AGG + chr8.1:15285739-15285758 MS.gene68979:intron 25.0%
!!! ATGAACTTGTTATGGAATTA+AGG + chr8.1:15284160-15284179 MS.gene68979:intron 25.0%
!!! CTTCAAAAATGAACTTGTTA+TGG + chr8.1:15284152-15284171 MS.gene68979:intron 25.0%
!!! TGAACTTGTTATGGAATTAA+GGG + chr8.1:15284161-15284180 MS.gene68979:intron 25.0%
!!! TGCCGTTTTTTCTTTTTTTT+TGG + chr8.1:15284556-15284575 MS.gene68979:intron 25.0%
AAATCTGCTATGCAATCATT+TGG + chr8.1:15285657-15285676 MS.gene68979:intron 30.0%
AACATTAGATTGCTCACAAA+GGG + chr8.1:15284792-15284811 MS.gene68979:intron 30.0%
ACAATTTGTGTTATGAATGC+CGG - chr8.1:15285310-15285329 None:intergenic 30.0%
ATCACTAAAACACAACAAGT+TGG - chr8.1:15285794-15285813 None:intergenic 30.0%
ATGGTAAAAAGACAGCAAAA+AGG - chr8.1:15284526-15284545 None:intergenic 30.0%
TAACATTAGATTGCTCACAA+AGG + chr8.1:15284791-15284810 MS.gene68979:intron 30.0%
TATGACATCAAATTAACCTC+AGG - chr8.1:15285211-15285230 None:intergenic 30.0%
TCCTTATCCTCACATAAAAT+CGG - chr8.1:15285028-15285047 None:intergenic 30.0%
! ATAACACAAATTGTGTTGAC+AGG + chr8.1:15285316-15285335 MS.gene68979:intron 30.0%
! GAATGTATGCTTTTCTATAC+TGG + chr8.1:15285367-15285386 MS.gene68979:intron 30.0%
! GCTTGAATGTTTTGTCAATT+TGG + chr8.1:15284630-15284649 MS.gene68979:intron 30.0%
AAAACGGCAGCAAATTTGTA+TGG - chr8.1:15284545-15284564 None:intergenic 35.0%
ACATGAGTGATGACATCAAT+AGG - chr8.1:15285004-15285023 None:intergenic 35.0%
AGCACTCAAGTTACAAAACA+AGG - chr8.1:15285277-15285296 None:intergenic 35.0%
CACAGTTTATCGAGAAAGAA+AGG + chr8.1:15285516-15285535 MS.gene68979:intron 35.0%
CTTGACAAAATGGTTACTCA+AGG + chr8.1:15284864-15284883 MS.gene68979:intron 35.0%
GAAAACAAGTGCATAAAGAG+AGG + chr8.1:15284713-15284732 MS.gene68979:CDS 35.0%
GGAGAGTTCTTGAAAATAAG+TGG + chr8.1:15284341-15284360 MS.gene68979:intron 35.0%
TAAAGAGAGATTAGGGAAAC+AGG - chr8.1:15285145-15285164 None:intergenic 35.0%
TAATATACAGGTCTATGAGC+TGG - chr8.1:15285478-15285497 None:intergenic 35.0%
TCTATGAGCTGGATTATAGA+TGG - chr8.1:15285467-15285486 None:intergenic 35.0%
TGAAATAAGCCAATCCAAAC+TGG + chr8.1:15284446-15284465 MS.gene68979:intron 35.0%
TTTCTTACACATATAGCCTG+AGG + chr8.1:15285192-15285211 MS.gene68979:intron 35.0%
! AACAAGCTTGAGTGTTTTCT+TGG - chr8.1:15283942-15283961 None:intergenic 35.0%
! AGGAGGTGGTTAAAAGTATA+AGG + chr8.1:15284741-15284760 MS.gene68979:CDS 35.0%
! CTTGAGTAACCATTTTGTCA+AGG - chr8.1:15284866-15284885 None:intergenic 35.0%
! TCATGTTCCGATTTTATGTG+AGG + chr8.1:15285018-15285037 MS.gene68979:CDS 35.0%
! TCCGATTTTATGTGAGGATA+AGG + chr8.1:15285024-15285043 MS.gene68979:CDS 35.0%
!! ACACTCAAGCTTGTTAAAAG+AGG + chr8.1:15283947-15283966 MS.gene68979:CDS 35.0%
AAAGGGTGAACTATCTCAAG+AGG + chr8.1:15285826-15285845 MS.gene68979:CDS 40.0%
AACTTTGCGCTGAATCTTGA+TGG + chr8.1:15285089-15285108 MS.gene68979:intron 40.0%
AAGGTGCAACATCATCAATG+AGG - chr8.1:15285258-15285277 None:intergenic 40.0%
AGTATAAGGCGAGGTCAAAA+AGG + chr8.1:15284755-15284774 MS.gene68979:CDS 40.0%
ATCAAGATTCAGCGCAAAGT+TGG - chr8.1:15285090-15285109 None:intergenic 40.0%
CATAAAGAGAGGAGTTAAGG+AGG + chr8.1:15284724-15284743 MS.gene68979:CDS 40.0%
GCAGAGTGTGTATTATAGCT+GGG + chr8.1:15284969-15284988 MS.gene68979:intron 40.0%
GCTTATTTCAGCTGATCTAC+TGG - chr8.1:15284436-15284455 None:intergenic 40.0%
GTATAAGGCGAGGTCAAAAA+GGG + chr8.1:15284756-15284775 MS.gene68979:CDS 40.0%
GTGCATAAAGAGAGGAGTTA+AGG + chr8.1:15284721-15284740 MS.gene68979:CDS 40.0%
TATGGAATTAAGGGCCTGTT+TGG + chr8.1:15284170-15284189 MS.gene68979:intron 40.0%
TGAGATAGTTCACCCTTTGA+AGG - chr8.1:15285824-15285843 None:intergenic 40.0%
! CTAAGCATTGCAGTGTTTCT+GGG + chr8.1:15284033-15284052 MS.gene68979:intron 40.0%
! GTGGTTAAAAGTATAAGGCG+AGG + chr8.1:15284746-15284765 MS.gene68979:CDS 40.0%
! TCTAAGCATTGCAGTGTTTC+TGG + chr8.1:15284032-15284051 MS.gene68979:intron 40.0%
! TTGAGTGCTTTTCCTTATGC+CGG + chr8.1:15285288-15285307 MS.gene68979:intron 40.0%
!!! TTGCAGTGTTTCTGGGTTTT+TGG + chr8.1:15284040-15284059 MS.gene68979:intron 40.0%
AAAGAGAGGAGTTAAGGAGG+TGG + chr8.1:15284727-15284746 MS.gene68979:CDS 45.0%
AGAGAGATTAGGGAAACAGG+CGG - chr8.1:15285142-15285161 None:intergenic 45.0%
AGTGATGACCAAGCCTTCAA+AGG + chr8.1:15285808-15285827 MS.gene68979:CDS 45.0%
ATTTGTCCAGGACCTTGCAA+CGG + chr8.1:15285751-15285770 MS.gene68979:intron 45.0%
CGACAGTGAAGGTGAGAAAT+CGG + chr8.1:15283841-15283860 MS.gene68979:CDS 45.0%
CGCAGAGTGTGTATTATAGC+TGG + chr8.1:15284968-15284987 MS.gene68979:intron 45.0%
GAAAGCTTCTTTCCAGCAAG+AGG - chr8.1:15283920-15283939 None:intergenic 45.0%
GTGATGACCAAGCCTTCAAA+GGG + chr8.1:15285809-15285828 MS.gene68979:CDS 45.0%
GTGTTATGAATGCCGGCATA+AGG - chr8.1:15285303-15285322 None:intergenic 45.0%
TAACATTCTTGGGGCCAGTT+TGG - chr8.1:15284463-15284482 None:intergenic 45.0%
! AGCAAGAGGTTTTGCAATGG+GGG - chr8.1:15283906-15283925 None:intergenic 45.0%
! CAGCAAGAGGTTTTGCAATG+GGG - chr8.1:15283907-15283926 None:intergenic 45.0%
! CCAGCAAGAGGTTTTGCAAT+GGG - chr8.1:15283908-15283927 None:intergenic 45.0%
! TAGTTCACCCTTTGAAGGCT+TGG - chr8.1:15285819-15285838 None:intergenic 45.0%
! TCCAGCAAGAGGTTTTGCAA+TGG - chr8.1:15283909-15283928 None:intergenic 45.0%
AAGGTGAGAAATCGGTGCAG+AGG + chr8.1:15283849-15283868 MS.gene68979:CDS 50.0%
AGGTGAGAAATCGGTGCAGA+GGG + chr8.1:15283850-15283869 MS.gene68979:CDS 50.0%
CACACTCTGCGTTGAGAAGA+AGG - chr8.1:15284959-15284978 None:intergenic 50.0%
CCCATTGCAAAACCTCTTGC+TGG + chr8.1:15283905-15283924 MS.gene68979:CDS 50.0%
GAAGGAGAGGAAGAAGATGC+AGG + chr8.1:15283874-15283893 MS.gene68979:CDS 50.0%
TTCTTGGGGCCAGTTTGGAT+TGG - chr8.1:15284458-15284477 None:intergenic 50.0%
GAAATCGGTGCAGAGGGAGA+AGG + chr8.1:15283856-15283875 MS.gene68979:CDS 55.0%
TCGTTGCTCCTGCCGTTGCA+AGG - chr8.1:15285766-15285785 None:intergenic 60.0%
CGGTGCAGAGGGAGAAGGAG+AGG + chr8.1:15283861-15283880 MS.gene68979:CDS 65.0%
CTCCTGCCGTTGCAAGGTCC+TGG - chr8.1:15285760-15285779 None:intergenic 65.0%
GTCCAGGACCTTGCAACGGC+AGG + chr8.1:15285755-15285774 MS.gene68979:intron 65.0%


Chromosome Type Strat End Strand Name
chr8.1 gene 15283833 15285922 15283833 ID=MS.gene68979
chr8.1 mRNA 15283833 15285922 15283833 ID=MS.gene68979.t1;Parent=MS.gene68979
chr8.1 exon 15283833 15283968 15283833 ID=MS.gene68979.t1.exon1;Parent=MS.gene68979.t1
chr8.1 CDS 15283833 15283968 15283833 ID=cds.MS.gene68979.t1;Parent=MS.gene68979.t1
chr8.1 exon 15284708 15284777 15284708 ID=MS.gene68979.t1.exon2;Parent=MS.gene68979.t1
chr8.1 CDS 15284708 15284777 15284708 ID=cds.MS.gene68979.t1;Parent=MS.gene68979.t1
chr8.1 exon 15284973 15285078 15284973 ID=MS.gene68979.t1.exon3;Parent=MS.gene68979.t1
chr8.1 CDS 15284973 15285078 15284973 ID=cds.MS.gene68979.t1;Parent=MS.gene68979.t1
chr8.1 exon 15285761 15285922 15285761 ID=MS.gene68979.t1.exon4;Parent=MS.gene68979.t1
chr8.1 CDS 15285761 15285922 15285761 ID=cds.MS.gene68979.t1;Parent=MS.gene68979.t1
Gene Sequence

>MS.gene68979

ATGGGAAGCGACAGTGAAGGTGAGAAATCGGTGCAGAGGGAGAAGGAGAGGAAGAAGATGCAGGCTCTAGCCCCCATTGCAAAACCTCTTGCTGGAAAGAAGCTTTCCAAGAAAACACTCAAGCTTGTTAAAAGAGGTATTTTCGAAGCTTTTAAACACTAGAGCTTGTTGAATTAATGAAAATCGTTAATTTGTGGCTTCTAAGCATTGCAGTGTTTCTGGGTTTTTGGAGTTAGTAAGAAAAATCACAACTTCTCTTTAATTTCAAAATGTATAATGCATCGAGTTTCTTATGTATGCTATGTAATTAGAGTATAAACTTCAAAAATGAACTTGTTATGGAATTAAGGGCCTGTTTGGATAAACAACTTATTTACAGCTTATAGCTCAAGCGCTTATCACACTTATGTACAAGCTTGCATAAGCTATGTTTATAGCAAAAGATTAAATTAACGTTAATTTGTTTTTGTATATGCCTAAATTGTTTTTATAAGCTATAAACTATATTGGAGAGTTCTTGAAAATAAGTGGACAAAAGCTTATGAAAATTGTTATCAGCTGTTTTTACAAATTCTCCAAAACAGACTTACAAAATGTATGCCAGTAGATCAGCTGAAATAAGCCAATCCAAACTGGCCCCAAGAATGTTATGTTATGAATCTAAGTATTATATTTTGCTGTCTGCTGTTACCTTTTTGCTGTCTTTTTACCATACAAATTTGCTGCCGTTTTTTCTTTTTTTTTGGAATGGCTAATGTTAATTTGTTGATTCTGTAATTTAAAAAGGAAAAAAATTAGCTTGAATGTTTTGTCAATTTGGATTACATAAATCTTTGTCTTATGGGACTATACTAATCTATGTGTGTTCTTTTCAGCTGCTGAAAACAAGTGCATAAAGAGAGGAGTTAAGGAGGTGGTTAAAAGTATAAGGCGAGGTCAAAAAGGGTCAGTATTAAGCTAACATTAGATTGCTCACAAAGGGCGTTAGAAATAATTAATTAATTCAAACTATGTTACTATCATTTATCTTCCTTGACAAAATGGTTACTCAAGGTGTATGTGAAGTGAATTCCCATTTTTTATGTTTCAATTGTGGAAAATAAATGTGTACACTTATTTTTTGCCTTCTTCTCAACGCAGAGTGTGTATTATAGCTGGGAACATATCGCCTATTGATGTCATCACTCATGTTCCGATTTTATGTGAGGATAAGGACATTCCATATGTATATGTCTCATCTAAAGAAGTAAGTAACCAACTTTGCGCTGAATCTTGATGGTTTTTATCATTTAGTTAATACTATTGACCGCCTGTTTCCCTAATCTCTCTTTAGAATAAGAAAAATTTGTTGTCATATATTTTCTTACACATATAGCCTGAGGTTAATTTGATGTCATAAATAACTAAAAGTTGAGGCTTTTTCCTCATTGATGATGTTGCACCTTGTTTTGTAACTTGAGTGCTTTTCCTTATGCCGGCATTCATAACACAAATTGTGTTGACAGGAGTTGAGTCCATATAACATATTTGAATGGAATGTATGCTTTTCTATACTGGATTGAATCATTTAGAGTATATCATACTTCAAGAAACATCTTTCAGCTATTTTAATTTTTTTATAGACACATCTGCCATCTATAATCCAGCTCATAGACCTGTATATTATGAATACAATAGTCATTGCACAGTTTATCGAGAAAGAAAGGATAATTTTTTGCTTCTCTTACCTACATAATATATCAACAATTCAACATGTGATGTTTTCAGTTTCGTTTTAAATATGGTATTAAGAGAAACATGGTTGAAATTGCTATTTTTCAAAAAAAATCTGCTATGCAATCATTTGGTGTTCATATATATGTTAAGAAACTCCAATTACTCTAGTACTTTGAAAGTTCATATATTAAATTTTTGAGATATTTGTCCAGGACCTTGCAACGGCAGGAGCAACGAAGAGACCAACTTGTTGTGTTTTAGTGATGACCAAGCCTTCAAAGGGTGAACTATCTCAAGAGGTACAAGAGAAACTAAAGTCAGAGTATGATCAAGTTGCATCAGAGTGTAGAGAGCTTCAGTCTACACTTTTTTGA

Protein sequence

>MS.gene68979.t1

MGSDSEGEKSVQREKERKKMQALAPIAKPLAGKKLSKKTLKLVKRAAENKCIKRGVKEVVKSIRRGQKGVCIIAGNISPIDVITHVPILCEDKDIPYVYVSSKEDLATAGATKRPTCCVLVMTKPSKGELSQEVQEKLKSEYDQVASECRELQSTLF