AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene29699

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene29699.t1 MTR_5g083360 98.413 567 9 0 1 567 1 567 0.0 1162
MS.gene29699.t1 MTR_3g071890 81.489 524 86 3 4 523 10 526 0.0 885
MS.gene29699.t1 MTR_5g073210 59.507 568 221 4 1 567 1 560 0.0 725
MS.gene29699.t1 MTR_5g020620 50.442 565 260 6 7 567 13 561 0.0 602
MS.gene29699.t1 MTR_5g020600 51.146 567 255 8 7 567 16 566 0.0 594
MS.gene29699.t1 MTR_7g062250 52.021 569 254 9 9 567 19 578 0.0 589
MS.gene29699.t1 MTR_2g008330 49.288 562 273 5 11 567 25 579 0.0 585
MS.gene29699.t1 MTR_7g058690 50.868 576 265 10 4 567 11 580 0.0 582
MS.gene29699.t1 MTR_7g062310 51.630 552 252 8 25 567 48 593 0.0 582
MS.gene29699.t1 MTR_4g064530 52.236 559 244 11 7 551 20 569 0.0 581
MS.gene29699.t1 MTR_5g069680 48.936 564 268 6 8 567 11 558 0.0 577
MS.gene29699.t1 MTR_4g015120 49.387 571 271 6 1 567 1 557 0.0 576
MS.gene29699.t1 MTR_7g458880 49.739 575 269 10 6 567 13 580 0.0 574
MS.gene29699.t1 MTR_7g060460 50.089 563 263 11 16 567 26 581 0.0 569
MS.gene29699.t1 MTR_3g101635 49.731 557 263 7 2 553 8 552 0.0 568
MS.gene29699.t1 MTR_3g101640 48.103 580 279 8 1 567 1 571 0.0 564
MS.gene29699.t1 MTR_3g462760 48.596 570 272 8 2 567 4 556 0.0 558
MS.gene29699.t1 MTR_5g081810 51.028 535 241 7 37 567 12 529 0.0 554
MS.gene29699.t1 MTR_7g065970 49.223 579 273 9 1 567 1 570 0.0 545
MS.gene29699.t1 MTR_4g064530 51.391 539 239 11 7 531 20 549 0.0 540
MS.gene29699.t1 MTR_4g019225 49.101 556 260 8 25 567 27 572 0.0 535
MS.gene29699.t1 MTR_7g065980 45.965 570 282 10 13 567 13 571 0.0 533
MS.gene29699.t1 MTR_8g027375 44.615 585 292 6 1 567 1 571 8.12e-179 517
MS.gene29699.t1 MTR_8g091390 31.058 586 320 20 19 567 29 567 1.78e-71 239
MS.gene29699.t1 MTR_3g078730 29.534 579 346 15 1 564 1 532 2.51e-67 228
MS.gene29699.t1 MTR_3g078740 30.820 610 348 21 1 564 1 582 3.26e-66 226
MS.gene29699.t1 MTR_3g078700 30.036 556 323 16 7 545 12 518 3.83e-66 224
MS.gene29699.t1 MTR_5g081790 42.857 287 142 7 243 525 152 420 2.60e-65 219
MS.gene29699.t1 MTR_5g081790 48.193 166 76 2 8 163 5 170 1.52e-46 169
MS.gene29699.t1 MTR_8g479390 27.856 499 301 15 38 527 45 493 4.09e-43 162
MS.gene29699.t1 MTR_4g101650 29.456 533 323 19 11 527 14 509 1.18e-42 161
MS.gene29699.t1 MTR_1g090707 26.288 563 378 13 7 567 9 536 8.15e-40 153
MS.gene29699.t1 MTR_3g008580 25.543 552 368 18 27 567 32 551 1.41e-39 152
MS.gene29699.t1 MTR_4g049390 27.108 498 299 14 42 527 45 490 4.92e-39 150
MS.gene29699.t1 MTR_8g073840 39.572 187 74 4 17 202 23 171 1.99e-37 140
MS.gene29699.t1 MTR_5g018270 27.219 507 291 16 38 527 45 490 6.62e-37 144
MS.gene29699.t1 MTR_1g026410 25.852 499 316 13 42 533 47 498 1.84e-36 143
MS.gene29699.t1 MTR_8g479380 27.843 510 291 18 42 533 47 497 1.86e-36 143
MS.gene29699.t1 MTR_4g075690 25.000 564 372 15 8 567 16 532 4.53e-36 142
MS.gene29699.t1 MTR_3g115050 25.992 504 309 15 42 533 48 499 1.55e-35 140
MS.gene29699.t1 MTR_2g088080 27.655 499 295 17 42 527 48 493 1.57e-35 140
MS.gene29699.t1 MTR_4g048350 27.108 498 298 18 42 527 45 489 5.38e-35 139
MS.gene29699.t1 MTR_7g051440 27.639 521 324 18 27 532 32 514 7.93e-34 136
MS.gene29699.t1 MTR_3g083430 25.000 544 347 15 38 567 39 535 3.72e-32 130
MS.gene29699.t1 MTR_3g083390 24.816 544 348 15 38 567 39 535 8.92e-32 129
MS.gene29699.t1 MTR_3g083450 24.816 544 348 15 38 567 40 536 2.67e-31 128
MS.gene29699.t1 MTR_3g083440 24.449 544 350 14 38 567 39 535 4.31e-31 127
MS.gene29699.t1 MTR_3g083400 24.815 540 353 14 38 567 39 535 5.81e-31 127
MS.gene29699.t1 MTR_4g083210 24.953 537 343 16 11 533 7 497 1.59e-30 125
MS.gene29699.t1 MTR_7g058670 60.494 81 31 1 81 160 13 93 4.01e-30 114
MS.gene29699.t1 MTR_3g083410 24.627 536 351 14 42 567 7 499 6.84e-30 123
MS.gene29699.t1 MTR_4g075690 25.314 478 307 14 8 481 16 447 1.72e-29 121
MS.gene29699.t1 MTR_4g132320 25.926 540 329 20 5 527 9 494 1.72e-29 122
MS.gene29699.t1 MTR_4g083190 25.520 529 355 14 42 567 45 537 3.44e-28 119
MS.gene29699.t1 MTR_2g088080 26.304 460 273 17 81 527 6 412 4.68e-25 108
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene29699.t1 AT2G40370 76.316 570 126 3 7 567 11 580 0.0 931
MS.gene29699.t1 AT5G05390 76.937 568 124 2 1 567 4 565 0.0 927
MS.gene29699.t1 AT2G30210 64.510 572 196 4 1 567 1 570 0.0 794
MS.gene29699.t1 AT5G07130 65.674 571 190 6 1 567 1 569 0.0 774
MS.gene29699.t1 AT5G07130 65.844 486 160 6 86 567 1 484 0.0 650
MS.gene29699.t1 AT5G07130 65.844 486 160 6 86 567 1 484 0.0 650
MS.gene29699.t1 AT5G09360 51.579 570 261 7 1 567 12 569 0.0 606
MS.gene29699.t1 AT2G29130 51.295 579 249 11 8 567 9 573 0.0 588
MS.gene29699.t1 AT5G60020 49.741 579 267 12 7 567 5 577 0.0 568
MS.gene29699.t1 AT5G03260 49.472 568 264 7 7 567 6 557 0.0 568
MS.gene29699.t1 AT5G60020 51.460 548 245 11 35 567 4 545 0.0 564
MS.gene29699.t1 AT2G38080 47.880 566 273 8 7 567 10 558 0.0 563
MS.gene29699.t1 AT5G01190 49.735 565 264 6 9 567 8 558 0.0 561
MS.gene29699.t1 AT3G09220 50.091 549 254 7 11 550 13 550 0.0 560
MS.gene29699.t1 AT5G58910 46.113 566 287 8 8 567 14 567 0.0 546
MS.gene29699.t1 AT5G01190 51.233 527 239 5 45 567 32 544 0.0 544
MS.gene29699.t1 AT5G58910 46.113 566 286 9 8 567 14 566 0.0 542
MS.gene29699.t1 AT5G48100 46.835 553 278 9 4 551 6 547 0.0 532
MS.gene29699.t1 AT2G46570 45.470 574 289 6 4 567 10 569 0.0 529
MS.gene29699.t1 AT1G18140 48.301 559 262 12 27 567 32 581 0.0 525
MS.gene29699.t1 AT5G01040 46.007 576 279 10 5 567 10 566 1.71e-180 521
MS.gene29699.t1 AT5G60020 49.534 537 247 12 7 525 5 535 7.60e-180 518
MS.gene29699.t1 AT5G58910 46.396 541 265 9 33 567 2 523 2.03e-179 516
MS.gene29699.t1 AT5G01050 45.391 575 286 8 5 567 10 568 7.31e-178 515
MS.gene29699.t1 AT3G09220 50.647 464 209 7 96 550 1 453 1.07e-162 472
MS.gene29699.t1 AT2G46570 46.465 495 243 5 81 567 4 484 1.13e-162 473
MS.gene29699.t1 AT5G21100 31.434 579 325 20 19 564 19 558 1.82e-73 245
MS.gene29699.t1 AT4G39830 30.921 608 338 22 1 567 11 577 1.57e-71 240
MS.gene29699.t1 AT5G21105 30.651 584 340 18 11 564 25 573 2.62e-69 234
MS.gene29699.t1 AT5G21105 30.092 545 320 17 11 525 25 538 3.14e-59 206
MS.gene29699.t1 AT4G39830 30.134 521 285 20 85 567 3 482 8.76e-52 185
MS.gene29699.t1 AT4G39830 30.000 520 285 20 86 567 2 480 5.54e-51 182
MS.gene29699.t1 AT4G39830 29.480 519 278 18 85 567 3 469 5.86e-49 177
MS.gene29699.t1 AT5G66920 26.443 537 331 14 42 567 55 538 5.91e-45 167
MS.gene29699.t1 AT4G12420 28.108 555 333 22 1 532 1 512 1.64e-44 167
MS.gene29699.t1 AT4G12420 28.108 555 333 22 1 532 1 512 1.64e-44 167
MS.gene29699.t1 AT1G75790 26.527 524 344 12 7 527 10 495 2.79e-42 160
MS.gene29699.t1 AT5G51480 26.560 561 371 17 17 567 21 550 2.00e-41 158
MS.gene29699.t1 AT1G21860 26.384 542 342 17 1 533 3 496 2.50e-39 151
MS.gene29699.t1 AT1G76160 24.811 528 354 15 7 533 11 496 4.40e-38 147
MS.gene29699.t1 AT4G25240 27.145 571 374 19 8 567 11 550 2.21e-37 146
MS.gene29699.t1 AT4G25240 27.145 571 374 19 8 567 11 550 2.21e-37 146
MS.gene29699.t1 AT1G21850 25.646 542 332 18 7 533 11 496 2.04e-36 143
MS.gene29699.t1 AT5G21105 29.781 366 215 11 208 564 50 382 2.89e-36 140
MS.gene29699.t1 AT1G41830 26.148 501 311 18 42 533 47 497 1.46e-35 140
MS.gene29699.t1 AT5G48450 25.532 564 364 20 9 564 13 528 1.46e-34 138
MS.gene29699.t1 AT5G48450 25.532 564 364 20 9 564 13 528 2.03e-34 138
MS.gene29699.t1 AT4G22010 26.404 534 330 18 6 527 8 490 6.68e-34 135
MS.gene29699.t1 AT2G23630 27.239 536 329 17 42 567 48 532 9.34e-31 126
MS.gene29699.t1 AT2G23630 27.239 536 329 17 42 567 53 537 1.08e-30 126
MS.gene29699.t1 AT4G37160 26.208 538 333 15 42 567 49 534 2.36e-30 125
MS.gene29699.t1 AT1G55560 25.047 535 334 18 42 564 44 523 9.92e-29 120
MS.gene29699.t1 AT1G55570 25.558 493 321 12 42 527 47 500 1.62e-28 120
MS.gene29699.t1 AT3G13390 25.391 512 332 12 25 527 29 499 6.60e-28 118
MS.gene29699.t1 AT4G28090 23.944 497 325 15 45 533 49 500 1.40e-27 117
MS.gene29699.t1 AT3G13400 25.301 498 315 15 42 527 45 497 6.72e-26 112
MS.gene29699.t1 AT3G13400 25.301 498 315 15 42 527 45 497 6.72e-26 112
MS.gene29699.t1 AT4G37160 30.466 279 178 4 42 319 49 312 1.92e-24 106
MS.gene29699.t1 AT4G38420 27.386 241 165 5 42 281 48 279 6.46e-20 93.2
MS.gene29699.t1 AT4G38420 27.386 241 165 5 42 281 48 279 1.19e-19 92.8
MS.gene29699.t1 AT4G38420 27.386 241 165 5 42 281 48 279 2.32e-19 92.0

Find 140 sgRNAs with CRISPR-Local

Find 239 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTAAAAGCTTTAGAAGTTTA+AGG 0.083519 5.3:-67544319 MS.gene29699:CDS
ACCTATAGATTTACCATTAA+TGG 0.101466 5.3:-67545715 MS.gene29699:CDS
CACCTACTACTGTTAGTTTA+TGG 0.135494 5.3:+67544621 None:intergenic
TGTACCTTCTTGTCCATTAA+TGG 0.180702 5.3:+67545702 None:intergenic
TGGCTTAGAGCCACAGTTTA+TGG 0.186697 5.3:-67545655 MS.gene29699:CDS
CGTGAAACACCCATTCTTCT+AGG 0.253813 5.3:-67545577 MS.gene29699:intron
ACAATTGTTCCAATTGATTC+AGG 0.259031 5.3:-67544710 MS.gene29699:CDS
TATATTGTTGCTGAGGGATT+TGG 0.270036 5.3:-67543897 MS.gene29699:CDS
ACTGTTGCTGTACCTGTTAA+TGG 0.271612 5.3:-67543813 MS.gene29699:CDS
TCTGATGCATACACTATTAA+TGG 0.273121 5.3:-67545152 MS.gene29699:CDS
CCAACTTTGGAAATTAATAA+TGG 0.283923 5.3:-67546591 MS.gene29699:CDS
AAAGTTACTAAGTTGAGATT+TGG 0.284792 5.3:-67544002 MS.gene29699:CDS
GGTGGCATGCTCATAGTTCA+TGG 0.302145 5.3:-67545675 MS.gene29699:CDS
TGAGGACCTGCCTTTGTGTT+AGG 0.313647 5.3:-67543132 None:intergenic
GTGCAGCATTGATTACTCTT+AGG 0.316195 5.3:+67544675 None:intergenic
CATTGATTTGCATTAAAATT+CGG 0.321328 5.3:+67544224 None:intergenic
GTGTATGCATCAGATATATT+TGG 0.328938 5.3:+67545161 None:intergenic
GGACATTGAGTCACAAATTC+TGG 0.334936 5.3:+67545760 None:intergenic
AGATCTTCATCAATTTCATT+AGG 0.335973 5.3:+67544284 None:intergenic
AGAGGATGGAGAAGGGCTAT+TGG 0.338124 5.3:-67543174 MS.gene29699:CDS
TGTTCACATTGGATGGGGTT+TGG 0.346069 5.3:-67543213 MS.gene29699:CDS
GTCCTCAGGAGGAGCTTCTA+TGG 0.350489 5.3:+67543148 None:intergenic
AATCACCCTATTCATCTTCA+TGG 0.351556 5.3:-67543930 MS.gene29699:CDS
TCATATCCATGAAGATGAAT+AGG 0.353366 5.3:+67543924 None:intergenic
CCATTATTAATTTCCAAAGT+TGG 0.356421 5.3:+67546591 None:intergenic
GTTTGATGTATGTAGGTGTT+TGG 0.364623 5.3:-67543256 MS.gene29699:intron
TTTGTTTCTCCTGAATCAAT+TGG 0.365718 5.3:+67544701 None:intergenic
CGCCGGGCGCGCTTACCAAT+CGG 0.373787 5.3:-67544489 MS.gene29699:CDS
GGATAAGCATCTCCTTCCTT+AGG 0.376333 5.3:+67545616 None:intergenic
AATTAATAATGGAGATACTT+TGG 0.377624 5.3:-67546580 MS.gene29699:CDS
GAGATTTGGATCAAGGGTTC+AGG 0.378810 5.3:-67543988 MS.gene29699:CDS
ACAACCAATGTCCTCATGTT+AGG 0.393555 5.3:-67544572 MS.gene29699:CDS
ACAATTTCCTGGTCCAACTT+TGG 0.400549 5.3:-67546604 MS.gene29699:CDS
ATAACTGCCCAACCATTAAC+AGG 0.400604 5.3:+67543801 None:intergenic
AGAATGGGTGTTTCACGCTT+TGG 0.403573 5.3:+67545583 None:intergenic
GGATTAGAACATCAGTGGTT+TGG 0.403888 5.3:+67544543 None:intergenic
CTAAGTTGAGATTTGGATCA+AGG 0.404145 5.3:-67543995 MS.gene29699:CDS
TGCTGTACCTGTTAATGGTT+GGG 0.408222 5.3:-67543808 MS.gene29699:CDS
TCCATTAATGGTAAATCTAT+AGG 0.411836 5.3:+67545714 None:intergenic
CAATGACCGACTCACATTAC+CGG 0.413180 5.3:+67544046 None:intergenic
AGCCGATTGGTAAGCGCGCC+CGG 0.414132 5.3:+67544487 None:intergenic
CCGGCGATGTAGTATCTTGA+TGG 0.420568 5.3:+67544506 None:intergenic
GTTACAATGTCACCATTCAT+TGG 0.426411 5.3:-67546533 MS.gene29699:intron
ACTGTGAATGGACAATTTCC+TGG 0.436760 5.3:-67546615 MS.gene29699:CDS
ATCCTGCAGGCTCATAAACT+TGG 0.437751 5.3:-67544128 MS.gene29699:CDS
CTTGGATGTTCACATTGGAT+GGG 0.442663 5.3:-67543219 MS.gene29699:CDS
ATGATCATTACCTAGAAGAA+TGG 0.444961 5.3:+67545567 None:intergenic
TAACTTTCTCCAGGACGAAT+AGG 0.449717 5.3:+67545739 None:intergenic
ACTTGGATGTTCACATTGGA+TGG 0.450150 5.3:-67543220 MS.gene29699:CDS
GCAGGTAATGAAGGCATGAT+TGG 0.454006 5.3:+67544374 None:intergenic
CATATCCATGAAGATGAATA+GGG 0.455044 5.3:+67543925 None:intergenic
GTGAGACAAGCCACACAAAC+AGG 0.461238 5.3:-67545191 MS.gene29699:CDS
GTGTTGCACAGCCTCTTCAC+TGG 0.461801 5.3:+67546651 None:intergenic
TCATTGTGGCAACCGATTCA+AGG 0.473488 5.3:-67544029 MS.gene29699:CDS
TCATCACCTTGTCCTGCAAA+GGG 0.474167 5.3:-67544413 MS.gene29699:CDS
TAACACAAAGGCAGGTCCTC+AGG 0.478994 5.3:+67543134 None:intergenic
TTGCTGTACCTGTTAATGGT+TGG 0.479566 5.3:-67543809 MS.gene29699:CDS
CAAATGAGCATCACTGTGAA+TGG 0.479962 5.3:-67546627 MS.gene29699:CDS
TGATGATTTGTATTCAAGAA+TGG 0.480556 5.3:+67544430 None:intergenic
ATCAATGTCAAGGACCGAAC+GGG 0.480946 5.3:-67544208 MS.gene29699:CDS
GTAACTTTAGTCCCTTGAAT+CGG 0.482864 5.3:+67544017 None:intergenic
AGATTTGTTGCTGACAATCC+AGG 0.489827 5.3:-67543777 MS.gene29699:intron
ACCTTGTTGATCCACCTATG+AGG 0.490977 5.3:-67543839 MS.gene29699:CDS
ACAGCAACAGTGTTCCTCAT+AGG 0.495749 5.3:+67543825 None:intergenic
TTGGATGTTCACATTGGATG+GGG 0.496559 5.3:-67543218 MS.gene29699:CDS
TTGGATTATGCATTGTCACT+TGG 0.496947 5.3:-67543237 MS.gene29699:CDS
AACCATAAACTAACAGTAGT+AGG 0.497147 5.3:-67544623 MS.gene29699:CDS
GGGGTTAGACAAATCAGAAC+AGG 0.499855 5.3:-67545796 MS.gene29699:CDS
GATAAGCATCTCCTTCCTTA+GGG 0.506054 5.3:+67545617 None:intergenic
GCTTGTCTCACAACATCCAT+AGG 0.506366 5.3:+67545203 None:intergenic
TGATCATTACCTAGAAGAAT+GGG 0.508193 5.3:+67545568 None:intergenic
ATGAGGACATTGGTTGTGAA+TGG 0.509668 5.3:+67544578 None:intergenic
TCACGCTTTGGCTTAGTGAA+AGG 0.510131 5.3:+67545595 None:intergenic
CTCCAAGTTTATGAGCCTGC+AGG 0.510477 5.3:+67544126 None:intergenic
AGAACATCAGTGGTTTGGCC+AGG 0.514268 5.3:+67544548 None:intergenic
ATCATCACCTTGTCCTGCAA+AGG 0.514426 5.3:-67544414 MS.gene29699:CDS
GTGATATTATTACCAATGAA+TGG 0.515384 5.3:+67546521 None:intergenic
AGATGAATAGGGTGATTCTC+AGG 0.517564 5.3:+67543936 None:intergenic
ATCAGAACAGGATGGGCTGA+TGG 0.518148 5.3:-67545784 MS.gene29699:CDS
TTGATGGTGGTTGGTCACCT+TGG 0.518654 5.3:+67544522 None:intergenic
TTTGCAATTGTGAAGAAAAG+AGG 0.519484 5.3:+67544644 None:intergenic
ACTCAATGTCCTATTCGTCC+TGG 0.520865 5.3:-67545748 MS.gene29699:CDS
GATTATCCTAACACAAAGGC+AGG 0.522293 5.3:+67543126 None:intergenic
GCTCATAAACTTGGAGTACA+AGG 0.524236 5.3:-67544119 MS.gene29699:CDS
CAGCATTGATTACTCTTAGG+AGG 0.526575 5.3:+67544678 None:intergenic
GAAGTGAACCGTGTCCCGTT+CGG 0.526770 5.3:+67544194 None:intergenic
TTTACCATTAATGGACAAGA+AGG 0.529526 5.3:-67545706 MS.gene29699:CDS
GTGTTTGTGTCATTGTAAGC+AGG 0.530023 5.3:+67544356 None:intergenic
ATGGTGGGATGCAAACCCTA+TGG 0.535899 5.3:-67545219 MS.gene29699:CDS
TCATTGTAAGCAGGTAATGA+AGG 0.540128 5.3:+67544365 None:intergenic
TGATTTCTATATTGTTGCTG+AGG 0.540678 5.3:-67543904 MS.gene29699:CDS
CTTTACAAGTGCTCTACCAA+AGG 0.542011 5.3:-67545116 MS.gene29699:intron
TTTAATGCAAATCAATGTCA+AGG 0.544640 5.3:-67544218 MS.gene29699:CDS
ATCACCTTGTCCTGCAAAGG+GGG 0.545026 5.3:-67544411 MS.gene29699:CDS
CTATAGGTGTAACTTTCTCC+AGG 0.545148 5.3:+67545730 None:intergenic
TAAGTTGAGATTTGGATCAA+GGG 0.547755 5.3:-67543994 MS.gene29699:CDS
CCATCAAGATACTACATCGC+CGG 0.549313 5.3:-67544506 MS.gene29699:CDS
GATTTCTATATTGTTGCTGA+GGG 0.549381 5.3:-67543903 MS.gene29699:CDS
TGGAGCTGCTCCTGTTTGTG+TGG 0.553190 5.3:+67545181 None:intergenic
CATTGTGGCAACCGATTCAA+GGG 0.556129 5.3:-67544028 MS.gene29699:CDS
ACCGGTGTAATCAAATTGCA+CGG 0.556156 5.3:+67544064 None:intergenic
AGCTCTAATCATTCACCCTA+AGG 0.560869 5.3:-67545632 MS.gene29699:CDS
CATCAAGATACTACATCGCC+GGG 0.561153 5.3:-67544505 MS.gene29699:CDS
ACCACTGATGTTCTAATCCA+AGG 0.563616 5.3:-67544539 MS.gene29699:CDS
TTAATTTCCAAAGTTGGACC+AGG 0.564251 5.3:+67546597 None:intergenic
GACAAGAAGGTACACTTTGG+TGG 0.574088 5.3:-67545693 MS.gene29699:CDS
CTTGTCTCACAACATCCATA+GGG 0.581965 5.3:+67545204 None:intergenic
TACACTATTAATGGTCAACC+TGG 0.583665 5.3:-67545143 MS.gene29699:CDS
GTCAAGGACCGAACGGGACA+CGG 0.584236 5.3:-67544202 MS.gene29699:CDS
CTAATCATTCACCCTAAGGA+AGG 0.586825 5.3:-67545628 MS.gene29699:CDS
TGTAGTATCTTGATGGTGGT+TGG 0.588008 5.3:+67544513 None:intergenic
GGTTGTGAATGGTTTGACAT+AGG 0.595120 5.3:+67544589 None:intergenic
ACCTTGGATTAGAACATCAG+TGG 0.604071 5.3:+67544538 None:intergenic
ATGGACAAGAAGGTACACTT+TGG 0.605647 5.3:-67545696 MS.gene29699:CDS
TTTGTATTCAAGAATGGCAG+TGG 0.605780 5.3:+67544436 None:intergenic
ATTGTTGAGTCCTAATCCAA+TGG 0.608848 5.3:+67544253 None:intergenic
TAGACAAATCAGAACAGGAT+GGG 0.611575 5.3:-67545791 MS.gene29699:CDS
AATCAATGTCAAGGACCGAA+CGG 0.611682 5.3:-67544209 MS.gene29699:CDS
TACAAGCAACTCCAGTGAAG+AGG 0.611814 5.3:-67546662 MS.gene29699:CDS
GAGCACTTGTAAAGATCACC+AGG 0.619435 5.3:+67545125 None:intergenic
GCAACAGTGTTCCTCATAGG+TGG 0.621280 5.3:+67543828 None:intergenic
TGTCACTTGGATGTTCACAT+TGG 0.623581 5.3:-67543224 MS.gene29699:CDS
GTGTAATCAAATTGCACGGG+AGG 0.625603 5.3:+67544068 None:intergenic
CCGGTGTAATCAAATTGCAC+GGG 0.626025 5.3:+67544065 None:intergenic
AATGTCCTCATGTTAGGACC+TGG 0.628341 5.3:-67544566 MS.gene29699:CDS
ATTACACCGGTAATGTGAGT+CGG 0.633330 5.3:-67544052 MS.gene29699:CDS
GTCCATAGAAGCTCCTCCTG+AGG 0.638740 5.3:-67543150 MS.gene29699:CDS
TGATTTGCATTAAAATTCGG+CGG 0.644510 5.3:+67544227 None:intergenic
CACAAAGGCAGGTCCTCAGG+AGG 0.658358 5.3:+67543137 None:intergenic
TCACCTTGTCCTGCAAAGGG+GGG 0.659071 5.3:-67544410 MS.gene29699:CDS
TTTGGATTATCCTAACACAA+AGG 0.661322 5.3:+67543122 None:intergenic
GTAATGTGAGTCGGTCATTG+TGG 0.663037 5.3:-67544043 MS.gene29699:CDS
TCCTCATAGGTGGATCAACA+AGG 0.663984 5.3:+67543838 None:intergenic
CCCGTGCAATTTGATTACAC+CGG 0.670640 5.3:-67544065 MS.gene29699:CDS
AGGTCCTAACATGAGGACAT+TGG 0.671705 5.3:+67544568 None:intergenic
CATCACCTTGTCCTGCAAAG+GGG 0.673225 5.3:-67544412 MS.gene29699:CDS
TTTGGCCAGGTCCTAACATG+AGG 0.676864 5.3:+67544561 None:intergenic
TTAGACAAATCAGAACAGGA+TGG 0.677485 5.3:-67545792 MS.gene29699:CDS
GATTAGAGCTCCATAAACTG+TGG 0.691733 5.3:+67545645 None:intergenic
GCGATGTAGTATCTTGATGG+TGG 0.696908 5.3:+67544509 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAAAAATTATAAATTTTATA+GGG - chr5.3:67546473-67546492 MS.gene29699:intron 0.0%
!!! ATTTTTTTAAAAAAAAAAAT+AGG + chr5.3:67543557-67543576 None:intergenic 0.0%
!! AAACTTTATCATAAAAATTA+AGG + chr5.3:67546516-67546535 None:intergenic 10.0%
!! ACTATAAAAATACTATTATA+TGG + chr5.3:67543992-67544011 None:intergenic 10.0%
!! ATTAACAACTAAAAATAAAA+AGG + chr5.3:67543250-67543269 None:intergenic 10.0%
!! CAATTAATTTATTATGTATA+TGG + chr5.3:67545101-67545120 None:intergenic 10.0%
!! TTAACAACTAAAAATAAAAA+GGG + chr5.3:67543249-67543268 None:intergenic 10.0%
!!! ACATAAATTTTATTCAAAAT+TGG - chr5.3:67546339-67546358 MS.gene29699:intron 10.0%
!!! ATAATAGTATTTTTATAGTA+GGG - chr5.3:67543993-67544012 MS.gene29699:CDS 10.0%
!!! ATGATGATTATTTTTTAATT+AGG - chr5.3:67544741-67544760 MS.gene29699:intron 10.0%
!!! ATTTTGAATAAAATTTATGT+AGG + chr5.3:67546339-67546358 None:intergenic 10.0%
!!! TATAATAGTATTTTTATAGT+AGG - chr5.3:67543992-67544011 MS.gene29699:CDS 10.0%
!!! TGATGATTATTTTTTAATTA+GGG - chr5.3:67544742-67544761 MS.gene29699:intron 10.0%
!!! TTTTTTCAATTTGTTAATTA+AGG - chr5.3:67544606-67544625 MS.gene29699:CDS 10.0%
!! AAAACTAAAAAGTAACTATA+TGG + chr5.3:67543887-67543906 None:intergenic 15.0%
!! AGATAAGAATTAAAATAAGT+TGG + chr5.3:67544564-67544583 None:intergenic 15.0%
!! AGTGTAATAACAAATTTAAT+TGG - chr5.3:67543738-67543757 MS.gene29699:intron 15.0%
!! GATAAGAATTAAAATAAGTT+GGG + chr5.3:67544563-67544582 None:intergenic 15.0%
!! GGAGATTAATTATAATTAAT+TGG - chr5.3:67543280-67543299 MS.gene29699:intron 15.0%
!! TAAAAATACTATTATATGGA+AGG + chr5.3:67543988-67544007 None:intergenic 15.0%
!! TCAAAAATCAAAAACAAAAA+AGG + chr5.3:67543941-67543960 None:intergenic 15.0%
!! TGCAAATAATCAATAATTTA+TGG - chr5.3:67544134-67544153 MS.gene29699:CDS 15.0%
!! TTATATTCCTATTAGTATAT+AGG - chr5.3:67543664-67543683 MS.gene29699:intron 15.0%
!!! ACCACAATTTAAAATTTTAA+TGG - chr5.3:67544068-67544087 MS.gene29699:CDS 15.0%
!!! ACCATTAAAATTTTAAATTG+TGG + chr5.3:67544072-67544091 None:intergenic 15.0%
!!! GATGATTATTTTTTAATTAG+GGG - chr5.3:67544743-67544762 MS.gene29699:intron 15.0%
!!! TAAGCAAAAATCAATATTTT+AGG + chr5.3:67545130-67545149 None:intergenic 15.0%
!!! TAGTTGTTAATTTAACTTTT+AGG - chr5.3:67543259-67543278 MS.gene29699:intron 15.0%
!! AAGAAAAATTGTTAAGAAGA+TGG + chr5.3:67543152-67543171 None:intergenic 20.0%
!! AATCTTTGTCTAAAAAGAAA+AGG + chr5.3:67544683-67544702 None:intergenic 20.0%
!! AATGATCATATGTCAAATAA+TGG + chr5.3:67545241-67545260 None:intergenic 20.0%
!! ACATAAAATAATCAAACCTT+TGG + chr5.3:67544889-67544908 None:intergenic 20.0%
!! ATGCATGCTATAATTTATAA+AGG - chr5.3:67544101-67544120 MS.gene29699:CDS 20.0%
!! ATGTTTGATCTTATGTAATT+TGG - chr5.3:67546705-67546724 MS.gene29699:intron 20.0%
!! ATTATTTGACATATGATCAT+TGG - chr5.3:67545240-67545259 MS.gene29699:intron 20.0%
!! GTCTCAATAAAATCAAAATA+AGG - chr5.3:67546414-67546433 MS.gene29699:intron 20.0%
!! TTGAATAAAATTTATGTAGG+AGG + chr5.3:67546336-67546355 None:intergenic 20.0%
!!! AATTAATAATGGAGATACTT+TGG - chr5.3:67543406-67543425 MS.gene29699:intron 20.0%
!!! ATTAGGGTTAATTATGTTTT+CGG - chr5.3:67546275-67546294 MS.gene29699:intron 20.0%
!!! CATTGATTTGCATTAAAATT+CGG + chr5.3:67545765-67545784 None:intergenic 20.0%
!!! TACTTTTCCTATATACTAAT+AGG + chr5.3:67543674-67543693 None:intergenic 20.0%
!!! TATATGGTCTTTTTAAATGT+CGG + chr5.3:67545085-67545104 None:intergenic 20.0%
!!! TTATTGCATTTTGAATCTTT+AGG - chr5.3:67544164-67544183 MS.gene29699:CDS 20.0%
!!! TTATTTTTTAATTAGGGGAA+TGG - chr5.3:67544748-67544767 MS.gene29699:intron 20.0%
!!! TTTTTGCTTATAATAGTGAT+CGG - chr5.3:67545140-67545159 MS.gene29699:CDS 20.0%
! AAAGTTTCACAAACATGATA+TGG - chr5.3:67546529-67546548 MS.gene29699:intron 25.0%
! AAGGTGTAAGAAAACAAAAA+AGG + chr5.3:67543969-67543988 None:intergenic 25.0%
! AATGGAAAAATGTTTAGTCA+TGG + chr5.3:67545223-67545242 None:intergenic 25.0%
! ACCTATAGATTTACCATTAA+TGG - chr5.3:67544271-67544290 MS.gene29699:CDS 25.0%
! AGAATTCAATAAGTGATTGA+AGG + chr5.3:67545187-67545206 None:intergenic 25.0%
! AGATCTTCATCAATTTCATT+AGG + chr5.3:67545705-67545724 None:intergenic 25.0%
! ATAAATAGCAATGCTCAAAA+AGG - chr5.3:67543803-67543822 MS.gene29699:CDS 25.0%
! CCATTATTAATTTCCAAAGT+TGG + chr5.3:67543398-67543417 None:intergenic 25.0%
! GGAAAAGTAAATGATAGATA+CGG - chr5.3:67543685-67543704 MS.gene29699:intron 25.0%
! GTGATATTATTACCAATGAA+TGG + chr5.3:67543468-67543487 None:intergenic 25.0%
! TAAATAGCAATGCTCAAAAA+GGG - chr5.3:67543804-67543823 MS.gene29699:CDS 25.0%
! TCCATTAATGGTAAATCTAT+AGG + chr5.3:67544275-67544294 None:intergenic 25.0%
! TTTAATGCAAATCAATGTCA+AGG - chr5.3:67545768-67545787 MS.gene29699:CDS 25.0%
!! AAAGTTACTAAGTTGAGATT+TGG - chr5.3:67545984-67546003 MS.gene29699:intron 25.0%
!! CCAACTTTGGAAATTAATAA+TGG - chr5.3:67543395-67543414 MS.gene29699:intron 25.0%
!! CTTAACAATTTTTCTTCTCT+TGG - chr5.3:67543156-67543175 MS.gene29699:CDS 25.0%
!! TGATGATTTGTATTCAAGAA+TGG + chr5.3:67545559-67545578 None:intergenic 25.0%
!!! AATTTTGAAGCATCTTTCTT+AGG + chr5.3:67546125-67546144 None:intergenic 25.0%
!!! ATTTTGAAGCATCTTTCTTA+GGG + chr5.3:67546124-67546143 None:intergenic 25.0%
!!! GTAAAAGCTTTAGAAGTTTA+AGG - chr5.3:67545667-67545686 MS.gene29699:CDS 25.0%
AAATGAGTTAAGACAAGTCA+GGG - chr5.3:67543855-67543874 MS.gene29699:CDS 30.0%
AACCATAAACTAACAGTAGT+AGG - chr5.3:67545363-67545382 MS.gene29699:intron 30.0%
ATCAAAATAAGGACTGAAAG+TGG - chr5.3:67546425-67546444 MS.gene29699:intron 30.0%
ATGATCATTACCTAGAAGAA+TGG + chr5.3:67544422-67544441 None:intergenic 30.0%
CATATCCATGAAGATGAATA+GGG + chr5.3:67546064-67546083 None:intergenic 30.0%
GACAAAGATTATGTTCATGA+TGG - chr5.3:67544693-67544712 MS.gene29699:CDS 30.0%
GATTTCTATATTGTTGCTGA+GGG - chr5.3:67546083-67546102 MS.gene29699:intron 30.0%
GGCATGTTTATTAATGTGAA+AGG - chr5.3:67543301-67543320 MS.gene29699:intron 30.0%
GTGTATGCATCAGATATATT+TGG + chr5.3:67544828-67544847 None:intergenic 30.0%
TAAATGAGTTAAGACAAGTC+AGG - chr5.3:67543854-67543873 MS.gene29699:CDS 30.0%
TCATATCCATGAAGATGAAT+AGG + chr5.3:67546065-67546084 None:intergenic 30.0%
TCTGATGCATACACTATTAA+TGG - chr5.3:67544834-67544853 MS.gene29699:intron 30.0%
TGATCATTACCTAGAAGAAT+GGG + chr5.3:67544421-67544440 None:intergenic 30.0%
TGATTTCTATATTGTTGCTG+AGG - chr5.3:67546082-67546101 MS.gene29699:intron 30.0%
TTTGCAATTGTGAAGAAAAG+AGG + chr5.3:67545345-67545364 None:intergenic 30.0%
TTTGGTTGTTTGATGTATGT+AGG - chr5.3:67546723-67546742 MS.gene29699:intron 30.0%
! ACAATTGTTCCAATTGATTC+AGG - chr5.3:67545276-67545295 MS.gene29699:intron 30.0%
! ATTTTGAATCTTTAGGCATG+GGG - chr5.3:67544171-67544190 MS.gene29699:CDS 30.0%
! CATTTTGAATCTTTAGGCAT+GGG - chr5.3:67544170-67544189 MS.gene29699:CDS 30.0%
! CTTTTCTTTACCATTGGATT+AGG - chr5.3:67545723-67545742 MS.gene29699:CDS 30.0%
! GAAGATCTTTTCTTTACCAT+TGG - chr5.3:67545717-67545736 MS.gene29699:CDS 30.0%
! TAAGTTGAGATTTGGATCAA+GGG - chr5.3:67545992-67546011 MS.gene29699:intron 30.0%
! TGATTTGCATTAAAATTCGG+CGG + chr5.3:67545762-67545781 None:intergenic 30.0%
! TTTACCATTAATGGACAAGA+AGG - chr5.3:67544280-67544299 MS.gene29699:CDS 30.0%
! TTTGTTTCTCCTGAATCAAT+TGG + chr5.3:67545288-67545307 None:intergenic 30.0%
!! TTTTTAATTAGGGGAATGGT+GGG - chr5.3:67544752-67544771 MS.gene29699:intron 30.0%
!!! TTTTTTAATTAGGGGAATGG+TGG - chr5.3:67544751-67544770 MS.gene29699:intron 30.0%
AAAAGGAATGTCACTCTACA+TGG + chr5.3:67543924-67543943 None:intergenic 35.0%
AATCACCCTATTCATCTTCA+TGG - chr5.3:67546056-67546075 MS.gene29699:intron 35.0%
ATTGTTGAGTCCTAATCCAA+TGG + chr5.3:67545736-67545755 None:intergenic 35.0%
GAAATTGAGCTAATGTTACC+TGG + chr5.3:67546230-67546249 None:intergenic 35.0%
GAGACTAAGTACATGACTAA+AGG + chr5.3:67546399-67546418 None:intergenic 35.0%
GCAGTCAAATGAAATTGATG+TGG + chr5.3:67546600-67546619 None:intergenic 35.0%
GTAACTTTAGTCCCTTGAAT+CGG + chr5.3:67545972-67545991 None:intergenic 35.0%
GTTACAATGTCACCATTCAT+TGG - chr5.3:67543453-67543472 MS.gene29699:intron 35.0%
GTTTGATGTATGTAGGTGTT+TGG - chr5.3:67546730-67546749 MS.gene29699:intron 35.0%
TACACTATTAATGGTCAACC+TGG - chr5.3:67544843-67544862 MS.gene29699:intron 35.0%
TAGACAAATCAGAACAGGAT+GGG - chr5.3:67544195-67544214 MS.gene29699:CDS 35.0%
TATATTGTTGCTGAGGGATT+TGG - chr5.3:67546089-67546108 MS.gene29699:intron 35.0%
TCATTGTAAGCAGGTAATGA+AGG + chr5.3:67545624-67545643 None:intergenic 35.0%
TCTTAGTACAAGTGTGAAGA+AGG + chr5.3:67543622-67543641 None:intergenic 35.0%
TGCAGGATTGAAAAATTGCT+AGG + chr5.3:67545846-67545865 None:intergenic 35.0%
TGTACCTTCTTGTCCATTAA+TGG + chr5.3:67544287-67544306 None:intergenic 35.0%
TTAATTTCCAAAGTTGGACC+AGG + chr5.3:67543392-67543411 None:intergenic 35.0%
TTAGACAAATCAGAACAGGA+TGG - chr5.3:67544194-67544213 MS.gene29699:CDS 35.0%
TTGCTTATAATAGTGATCGG+AGG - chr5.3:67545143-67545162 MS.gene29699:CDS 35.0%
TTGGATTATGCATTGTCACT+TGG - chr5.3:67546749-67546768 MS.gene29699:intron 35.0%
TTTGTATTCAAGAATGGCAG+TGG + chr5.3:67545553-67545572 None:intergenic 35.0%
! CACCTACTACTGTTAGTTTA+TGG + chr5.3:67545368-67545387 None:intergenic 35.0%
! CTAAGTTGAGATTTGGATCA+AGG - chr5.3:67545991-67546010 MS.gene29699:intron 35.0%
! GATTATGTTCATGATGGAGA+TGG - chr5.3:67544699-67544718 MS.gene29699:CDS 35.0%
! GCATTTTGAATCTTTAGGCA+TGG - chr5.3:67544169-67544188 MS.gene29699:CDS 35.0%
! TCATGATGGAGATGGTTTAA+CGG - chr5.3:67544707-67544726 MS.gene29699:CDS 35.0%
!! AAAAAGTGTTTGCTTGCTTG+TGG + chr5.3:67543595-67543614 None:intergenic 35.0%
!! AAAAGTGTTTGCTTGCTTGT+GGG + chr5.3:67543594-67543613 None:intergenic 35.0%
!! AGTACAAGGTGTTTTTACGA+CGG - chr5.3:67545881-67545900 MS.gene29699:intron 35.0%
!! TAGCAATTTTTCAATCCTGC+AGG - chr5.3:67545845-67545864 MS.gene29699:intron 35.0%
AATCAATGTCAAGGACCGAA+CGG - chr5.3:67545777-67545796 MS.gene29699:CDS 40.0%
AATTGATGTGGCCTCAGTTA+TGG + chr5.3:67546588-67546607 None:intergenic 40.0%
ACAACCAATGTCCTCATGTT+AGG - chr5.3:67545414-67545433 MS.gene29699:intron 40.0%
ACAATTTCCTGGTCCAACTT+TGG - chr5.3:67543382-67543401 MS.gene29699:intron 40.0%
ACCACTGATGTTCTAATCCA+AGG - chr5.3:67545447-67545466 MS.gene29699:intron 40.0%
ACCGGTGTAATCAAATTGCA+CGG + chr5.3:67545925-67545944 None:intergenic 40.0%
ACTGTGAATGGACAATTTCC+TGG - chr5.3:67543371-67543390 MS.gene29699:intron 40.0%
ACTGTTGCTGTACCTGTTAA+TGG - chr5.3:67546173-67546192 MS.gene29699:intron 40.0%
ACTTGGATGTTCACATTGGA+TGG - chr5.3:67546766-67546785 MS.gene29699:intron 40.0%
AGATGAATAGGGTGATTCTC+AGG + chr5.3:67546053-67546072 None:intergenic 40.0%
ATAACTGCCCAACCATTAAC+AGG + chr5.3:67546188-67546207 None:intergenic 40.0%
ATTACACCGGTAATGTGAGT+CGG - chr5.3:67545934-67545953 MS.gene29699:intron 40.0%
CAAATGAGCATCACTGTGAA+TGG - chr5.3:67543359-67543378 MS.gene29699:intron 40.0%
CAGGACCAAGTATCTATATG+CGG - chr5.3:67546629-67546648 MS.gene29699:CDS 40.0%
CATATGATCATTGGCAATGC+AGG - chr5.3:67545249-67545268 MS.gene29699:intron 40.0%
CTAATCATTCACCCTAAGGA+AGG - chr5.3:67544358-67544377 MS.gene29699:CDS 40.0%
CTATAGGTGTAACTTTCTCC+AGG + chr5.3:67544259-67544278 None:intergenic 40.0%
CTTGGATGTTCACATTGGAT+GGG - chr5.3:67546767-67546786 MS.gene29699:intron 40.0%
CTTGTCTCACAACATCCATA+GGG + chr5.3:67544785-67544804 None:intergenic 40.0%
CTTTACAAGTGCTCTACCAA+AGG - chr5.3:67544870-67544889 MS.gene29699:intron 40.0%
GATAAGCATCTCCTTCCTTA+GGG + chr5.3:67544372-67544391 None:intergenic 40.0%
GATTAGAGCTCCATAAACTG+TGG + chr5.3:67544344-67544363 None:intergenic 40.0%
GCGGTTGCGATATAAAACTT+TGG - chr5.3:67546648-67546667 MS.gene29699:CDS 40.0%
GCTCATAAACTTGGAGTACA+AGG - chr5.3:67545867-67545886 MS.gene29699:intron 40.0%
GGACATTGAGTCACAAATTC+TGG + chr5.3:67544229-67544248 None:intergenic 40.0%
GGATTAGAACATCAGTGGTT+TGG + chr5.3:67545446-67545465 None:intergenic 40.0%
GGTTGTGAATGGTTTGACAT+AGG + chr5.3:67545400-67545419 None:intergenic 40.0%
TAACTTTCTCCAGGACGAAT+AGG + chr5.3:67544250-67544269 None:intergenic 40.0%
TGCTGTACCTGTTAATGGTT+GGG - chr5.3:67546178-67546197 MS.gene29699:intron 40.0%
TTGCTGTACCTGTTAATGGT+TGG - chr5.3:67546177-67546196 MS.gene29699:intron 40.0%
TTGGATGTTCACATTGGATG+GGG - chr5.3:67546768-67546787 MS.gene29699:intron 40.0%
! ACCTTGGATTAGAACATCAG+TGG + chr5.3:67545451-67545470 None:intergenic 40.0%
! AGCTCTAATCATTCACCCTA+AGG - chr5.3:67544354-67544373 MS.gene29699:CDS 40.0%
! ATGAGGACATTGGTTGTGAA+TGG + chr5.3:67545411-67545430 None:intergenic 40.0%
! ATGGACAAGAAGGTACACTT+TGG - chr5.3:67544290-67544309 MS.gene29699:CDS 40.0%
! GTGTTTGTGTCATTGTAAGC+AGG + chr5.3:67545633-67545652 None:intergenic 40.0%
! TGTCACTTGGATGTTCACAT+TGG - chr5.3:67546762-67546781 MS.gene29699:intron 40.0%
! TGTTTCTGTGACCATAACTG+AGG - chr5.3:67546574-67546593 MS.gene29699:CDS 40.0%
! TTTTGGTAGAGGATGGAGAA+GGG - chr5.3:67546805-67546824 MS.gene29699:CDS 40.0%
!! AGATTTGTTGCTGACAATCC+AGG - chr5.3:67546209-67546228 MS.gene29699:intron 40.0%
!! CAGCATTGATTACTCTTAGG+AGG + chr5.3:67545311-67545330 None:intergenic 40.0%
!! GTGCAGCATTGATTACTCTT+AGG + chr5.3:67545314-67545333 None:intergenic 40.0%
!! TGTAGTATCTTGATGGTGGT+TGG + chr5.3:67545476-67545495 None:intergenic 40.0%
!! TTTTTGGTAGAGGATGGAGA+AGG - chr5.3:67546804-67546823 MS.gene29699:CDS 40.0%
!!! ACTGTGTTTTTGGTAGAGGA+TGG - chr5.3:67546798-67546817 MS.gene29699:CDS 40.0%
AATGTCCTCATGTTAGGACC+TGG - chr5.3:67545420-67545439 MS.gene29699:intron 45.0%
ACAGCAACAGTGTTCCTCAT+AGG + chr5.3:67546164-67546183 None:intergenic 45.0%
ACCTTGTTGATCCACCTATG+AGG - chr5.3:67546147-67546166 MS.gene29699:intron 45.0%
ACTCAATGTCCTATTCGTCC+TGG - chr5.3:67544238-67544257 MS.gene29699:CDS 45.0%
AGAATGGGTGTTTCACGCTT+TGG + chr5.3:67544406-67544425 None:intergenic 45.0%
AGGTCCTAACATGAGGACAT+TGG + chr5.3:67545421-67545440 None:intergenic 45.0%
ATCAATGTCAAGGACCGAAC+GGG - chr5.3:67545778-67545797 MS.gene29699:CDS 45.0%
ATCATCACCTTGTCCTGCAA+AGG - chr5.3:67545572-67545591 MS.gene29699:intron 45.0%
ATCCTGCAGGCTCATAAACT+TGG - chr5.3:67545858-67545877 MS.gene29699:intron 45.0%
ATTTGACTGCGATTCTCTGC+AGG - chr5.3:67546610-67546629 MS.gene29699:CDS 45.0%
CATCAAGATACTACATCGCC+GGG - chr5.3:67545481-67545500 MS.gene29699:intron 45.0%
CCATCAAGATACTACATCGC+CGG - chr5.3:67545480-67545499 MS.gene29699:intron 45.0%
CCCGTGCAATTTGATTACAC+CGG - chr5.3:67545921-67545940 MS.gene29699:intron 45.0%
CCGGTGTAATCAAATTGCAC+GGG + chr5.3:67545924-67545943 None:intergenic 45.0%
CGCAACCGCATATAGATACT+TGG + chr5.3:67546637-67546656 None:intergenic 45.0%
CGTGAAACACCCATTCTTCT+AGG - chr5.3:67544409-67544428 MS.gene29699:CDS 45.0%
GAGATTTGGATCAAGGGTTC+AGG - chr5.3:67545998-67546017 MS.gene29699:intron 45.0%
GAGCACTTGTAAAGATCACC+AGG + chr5.3:67544864-67544883 None:intergenic 45.0%
GCGATGTAGTATCTTGATGG+TGG + chr5.3:67545480-67545499 None:intergenic 45.0%
GCTTGTCTCACAACATCCAT+AGG + chr5.3:67544786-67544805 None:intergenic 45.0%
GGATAAGCATCTCCTTCCTT+AGG + chr5.3:67544373-67544392 None:intergenic 45.0%
GGGGTTAGACAAATCAGAAC+AGG - chr5.3:67544190-67544209 MS.gene29699:CDS 45.0%
GTAATGTGAGTCGGTCATTG+TGG - chr5.3:67545943-67545962 MS.gene29699:intron 45.0%
GTGTAATCAAATTGCACGGG+AGG + chr5.3:67545921-67545940 None:intergenic 45.0%
TACAAGCAACTCCAGTGAAG+AGG - chr5.3:67543324-67543343 MS.gene29699:intron 45.0%
TCATCACCTTGTCCTGCAAA+GGG - chr5.3:67545573-67545592 MS.gene29699:intron 45.0%
TCCTCATAGGTGGATCAACA+AGG + chr5.3:67546151-67546170 None:intergenic 45.0%
TGGCTTAGAGCCACAGTTTA+TGG - chr5.3:67544331-67544350 MS.gene29699:CDS 45.0%
TGTTCACATTGGATGGGGTT+TGG - chr5.3:67546773-67546792 MS.gene29699:intron 45.0%
! AAATTGCACGGGAGGTTTTG+TGG + chr5.3:67545913-67545932 None:intergenic 45.0%
! CAATGACCGACTCACATTAC+CGG + chr5.3:67545943-67545962 None:intergenic 45.0%
! CATTGTGGCAACCGATTCAA+GGG - chr5.3:67545958-67545977 MS.gene29699:intron 45.0%
! GACAAGAAGGTACACTTTGG+TGG - chr5.3:67544293-67544312 MS.gene29699:CDS 45.0%
! GCAGGTAATGAAGGCATGAT+TGG + chr5.3:67545615-67545634 None:intergenic 45.0%
! GGGTTTGGCTACTGTGTTTT+TGG - chr5.3:67546788-67546807 MS.gene29699:intron 45.0%
! TCACGCTTTGGCTTAGTGAA+AGG + chr5.3:67544394-67544413 None:intergenic 45.0%
! TCATTGTGGCAACCGATTCA+AGG - chr5.3:67545957-67545976 MS.gene29699:intron 45.0%
!! AATTGCACGGGAGGTTTTGT+GGG + chr5.3:67545912-67545931 None:intergenic 45.0%
!!! GGCTACTGTGTTTTTGGTAG+AGG - chr5.3:67546794-67546813 MS.gene29699:CDS 45.0%
!! ATTGTATAAAAAATATAATT+AGG - chr5.3:67546258-67546277 MS.gene29699:intron 5.0%
!! TGTAAAAATTAATAAAAATA+GGG + chr5.3:67546366-67546385 None:intergenic 5.0%
!! TTGTAAAAATTAATAAAAAT+AGG + chr5.3:67546367-67546386 None:intergenic 5.0%
!! TTGTATAAAAAATATAATTA+GGG - chr5.3:67546259-67546278 MS.gene29699:intron 5.0%
!!! GAAAAAATTATAAATTTTAT+AGG - chr5.3:67546472-67546491 MS.gene29699:intron 5.0%
!!! TTTTATTAATTTTTACAAAA+TGG - chr5.3:67546369-67546388 MS.gene29699:intron 5.0%
AGAGGATGGAGAAGGGCTAT+TGG - chr5.3:67546812-67546831 MS.gene29699:CDS 50.0%
ATATTTGCACCCCCCTTTGC+AGG + chr5.3:67545588-67545607 None:intergenic 50.0%
ATCACCTTGTCCTGCAAAGG+GGG - chr5.3:67545575-67545594 MS.gene29699:intron 50.0%
CATCACCTTGTCCTGCAAAG+GGG - chr5.3:67545574-67545593 MS.gene29699:intron 50.0%
CCGGCGATGTAGTATCTTGA+TGG + chr5.3:67545483-67545502 None:intergenic 50.0%
CTCCAAGTTTATGAGCCTGC+AGG + chr5.3:67545863-67545882 None:intergenic 50.0%
GCAACAGTGTTCCTCATAGG+TGG + chr5.3:67546161-67546180 None:intergenic 50.0%
GGTGGCATGCTCATAGTTCA+TGG - chr5.3:67544311-67544330 MS.gene29699:CDS 50.0%
GTGAGACAAGCCACACAAAC+AGG - chr5.3:67544795-67544814 MS.gene29699:intron 50.0%
TAACACAAAGGCAGGTCCTC+AGG + chr5.3:67546855-67546874 None:intergenic 50.0%
TTTGGCCAGGTCCTAACATG+AGG + chr5.3:67545428-67545447 None:intergenic 50.0%
! ACGCAGCATTTTGAGCCGAT+TGG + chr5.3:67545515-67545534 None:intergenic 50.0%
! AGAACATCAGTGGTTTGGCC+AGG + chr5.3:67545441-67545460 None:intergenic 50.0%
! ATGGTGGGATGCAAACCCTA+TGG - chr5.3:67544767-67544786 MS.gene29699:intron 50.0%
!! ATCAGAACAGGATGGGCTGA+TGG - chr5.3:67544202-67544221 MS.gene29699:CDS 50.0%
!! TTGATGGTGGTTGGTCACCT+TGG + chr5.3:67545467-67545486 None:intergenic 50.0%
GAAGTGAACCGTGTCCCGTT+CGG + chr5.3:67545795-67545814 None:intergenic 55.0%
GTCCATAGAAGCTCCTCCTG+AGG - chr5.3:67546836-67546855 MS.gene29699:CDS 55.0%
GTCCTCAGGAGGAGCTTCTA+TGG + chr5.3:67546841-67546860 None:intergenic 55.0%
GTGTTGCACAGCCTCTTCAC+TGG + chr5.3:67543338-67543357 None:intergenic 55.0%
TCACCTTGTCCTGCAAAGGG+GGG - chr5.3:67545576-67545595 MS.gene29699:intron 55.0%
TGGAGCTGCTCCTGTTTGTG+TGG + chr5.3:67544808-67544827 None:intergenic 55.0%
CACAAAGGCAGGTCCTCAGG+AGG + chr5.3:67546852-67546871 None:intergenic 60.0%
GTCAAGGACCGAACGGGACA+CGG - chr5.3:67545784-67545803 MS.gene29699:CDS 60.0%
AGCCGATTGGTAAGCGCGCC+CGG + chr5.3:67545502-67545521 None:intergenic 65.0%
GCACCCCCCTTTGCAGGACA+AGG + chr5.3:67545582-67545601 None:intergenic 65.0%
CGCCGGGCGCGCTTACCAAT+CGG - chr5.3:67545497-67545516 MS.gene29699:intron 70.0%


Chromosome Type Strat End Strand Name
chr5.3 gene 67543133 67546875 67543133 ID=MS.gene29699
chr5.3 mRNA 67543133 67546875 67543133 ID=MS.gene29699.t1;Parent=MS.gene29699
chr5.3 exon 67546789 67546875 67546789 ID=MS.gene29699.t1.exon1;Parent=MS.gene29699.t1
chr5.3 CDS 67546789 67546875 67546789 ID=cds.MS.gene29699.t1;Parent=MS.gene29699.t1
chr5.3 exon 67546534 67546685 67546534 ID=MS.gene29699.t1.exon2;Parent=MS.gene29699.t1
chr5.3 CDS 67546534 67546685 67546534 ID=cds.MS.gene29699.t1;Parent=MS.gene29699.t1
chr5.3 exon 67545578 67545822 67545578 ID=MS.gene29699.t1.exon3;Parent=MS.gene29699.t1
chr5.3 CDS 67545578 67545822 67545578 ID=cds.MS.gene29699.t1;Parent=MS.gene29699.t1
chr5.3 exon 67545117 67545245 67545117 ID=MS.gene29699.t1.exon4;Parent=MS.gene29699.t1
chr5.3 CDS 67545117 67545245 67545117 ID=cds.MS.gene29699.t1;Parent=MS.gene29699.t1
chr5.3 exon 67543778 67544737 67543778 ID=MS.gene29699.t1.exon5;Parent=MS.gene29699.t1
chr5.3 CDS 67543778 67544737 67543778 ID=cds.MS.gene29699.t1;Parent=MS.gene29699.t1
chr5.3 exon 67543133 67543263 67543133 ID=MS.gene29699.t1.exon6;Parent=MS.gene29699.t1
chr5.3 CDS 67543133 67543263 67543133 ID=cds.MS.gene29699.t1;Parent=MS.gene29699.t1
Gene Sequence

>MS.gene29699

ATGGAAGCTTTCAACACCATCTTCTTAACAATTTTTCTTCTCTTGGCTTCTTTAGCTAATGCTAAAGTTCATGAGCATGAGTTTGTCGTAAGTTTTTTTATTTTTTTTTAAAGCCCTTTTTATTTTTAGTTGTTAATTTAACTTTTAGGAGATTAATTATAATTAATTGGCATGTTTATTAATGTGAAAGGTACAAGCAACTCCAGTGAAGAGGCTGTGCAACACACAAATGAGCATCACTGTGAATGGACAATTTCCTGGTCCAACTTTGGAAATTAATAATGGAGATACTTTGGTTGTCAAAGTCATTAACAAAGCTCGTTACAATGTCACCATTCATTGGTAATAATATCACAAGCTAGCTAGAACTTTGTATATTAATTTTTATTTTATTTTGTTAACAAATTAATAGCTCACTTTGCCTATTTTTTTTTTTAAAAAAATATTAGATCACTTTACCCACAAGCAAGCAAACACTTTTTATATCCTTCTTCACACTTGTACTAAGAATTGATTGCAAGCTCACATTAATTATATTCCTATTAGTATATAGGAAAAGTAAATGATAGATACGGTGACAAAATTTATCAAGAAAATTTCAAGACAGTGTAATAACAAATTTAATTGGTTGAGACATTTTCTTGATAAATTCTTGATAAAAACTCTCTAGATAAATAGCAATGCTCAAAAAGGGAAATATATTAAGCATCATGATTGTGAATAAATGAGTTAAGACAAGTCAGGGTATATACCATATAGTTACTTTTTAGTTTTCTAAATATGATCAACCATGTAGAGTGACATTCCTTTTTTGTTTTTGATTTTTGAAATTTCCTTTTTTGTTTTCTTACACCTTCCATATAATAGTATTTTTATAGTAGGGTATGCAATTTGAGCTGTATATTTTTGAAAAAAAAAAGTCAAGATTATTTACAACCACAATTTAAAATTTTAATGGTATGTTTTTCATGCATGCTATAATTTATAAAGGATCATATATATGCAAATAATCAATAATTTATGGCTTGATTTTATTGCATTTTGAATCTTTAGGCATGGGGTTAGACAAATCAGAACAGGATGGGCTGATGGACCAGAATTTGTGACTCAATGTCCTATTCGTCCTGGAGAAAGTTACACCTATAGATTTACCATTAATGGACAAGAAGGTACACTTTGGTGGCATGCTCATAGTTCATGGCTTAGAGCCACAGTTTATGGAGCTCTAATCATTCACCCTAAGGAAGGAGATGCTTATCCTTTCACTAAGCCAAAGCGTGAAACACCCATTCTTCTAGGTAATGATCATTGTATTTGACATCTAACTTTTTTTTTTAAGAACTTTGCAAACGTGATTATTAGATTTTTATATCTGATGTAACTGATGTGTGTAACATCGACTTGTAAAATGAAGAGTGTCATTCTTTCCCAACTTATTTTAATTCTTATCTTTATTTAACGTGTGACTTAGATTTTTTTCAATTTGTTAATTAAGGATGTGATTGATATGAAATCATAAAGAAAATGAAATTATGAGATATGTTTGTCCTTTTCTTTTTAGACAAAGATTATGTTCATGATGGAGATGGTTTAACGGTGAAAATAATTATGATGATTATTTTTTAATTAGGGGAATGGTGGGATGCAAACCCTATGGATGTTGTGAGACAAGCCACACAAACAGGAGCAGCTCCAAATATATCTGATGCATACACTATTAATGGTCAACCTGGTGATCTTTACAAGTGCTCTACCAAAGGTTTGATTATTTTATGTTATTTTTTAATTTTGAGTAGATTTTTTAGTGAACTCATTATCAACATGTCACATATATGATTGATTAATTAATAAATTAAATAATTTTTTTATAAATTTACTATAGTCGAATATTTTATTTTAATTAGTTATAAGACAATCGTCAACAATTTTTCATAAAAAAAAAAAAGTAACCGACATTTAAAAAGACCATATACATAATAAATTAATTGAATGAACCTAAAATATTGATTTTTGCTTATAATAGTGATCGGAGGATGTATGCAATAATAACACCTTCAATCACTTATTGAATTCTTCATGATATATGACCATGACTAAACATTTTTCCATTATTTGACATATGATCATTGGCAATGCAGGCACAACAATTGTTCCAATTGATTCAGGAGAAACAAACCTCCTAAGAGTAATCAATGCTGCACTAAATCAACCTCTTTTCTTCACAATTGCAAACCATAAACTAACAGTAGTAGGTGCTGATGCTTCCTATGTCAAACCATTCACAACCAATGTCCTCATGTTAGGACCTGGCCAAACCACTGATGTTCTAATCCAAGGTGACCAACCACCATCAAGATACTACATCGCCGGGCGCGCTTACCAATCGGCTCAAAATGCTGCGTTCGATAACACAACAACCACTGCCATTCTTGAATACAAATCATCACCTTGTCCTGCAAAGGGGGGTGCAAATATCAGACCAATCATGCCTTCATTACCTGCTTACAATGACACAAACACTGTTACATCATTTAGTAAAAGCTTTAGAAGTTTAAGGAATGTTGAAGTTCCTAATGAAATTGATGAAGATCTTTTCTTTACCATTGGATTAGGACTCAACAATTGTCCGCCGAATTTTAATGCAAATCAATGTCAAGGACCGAACGGGACACGGTTCACTTCTAGTATGAACAATGTGTCTTTTGTACTTCCTAGCAATTTTTCAATCCTGCAGGCTCATAAACTTGGAGTACAAGGTGTTTTTACGACGGATTTTCCCACAAAACCTCCCGTGCAATTTGATTACACCGGTAATGTGAGTCGGTCATTGTGGCAACCGATTCAAGGGACTAAAGTTACTAAGTTGAGATTTGGATCAAGGGTTCAGGTTGTTTTGCAAGATACTAGTATTGTTACTCCTGAGAATCACCCTATTCATCTTCATGGATATGATTTCTATATTGTTGCTGAGGGATTTGGAAATTATGACCCTAAGAAAGATGCTTCAAAATTTAACCTTGTTGATCCACCTATGAGGAACACTGTTGCTGTACCTGTTAATGGTTGGGCAGTTATCAGATTTGTTGCTGACAATCCAGGTAACATTAGCTCAATTTCAACTTTGCATTGTATAAAAAATATAATTAGGGTTAATTATGTTTTCGGTCTCTAAAAAATTAAGATTTTTTTTAAGTTTAATTCCTCCTACATAAATTTTATTCAAAATTGGTCCCTATTTTTATTAATTTTTACAAAATGGAGTACCTTTAGTCATGTACTTAGTCTCAATAAAATCAAAATAAGGACTGAAAGTGGACAATTTACGAAAAGATTAAACTCGAAAAAATTATAAATTTTATAGGGATTAAAAATATATTTAACCTTAATTTTTATGATAAAGTTTCACAAACATGATATGGATCGTGACATCAATGTCTTTGATGTTTCTGTGACCATAACTGAGGCCACATCAATTTCATTTGACTGCGATTCTCTGCAGGACCAAGTATCTATATGCGGTTGCGATATAAAACTTTGGTATAATGAAGAGAAAAAACATAATCAATTATTCAATGTTTGATCTTATGTAATTTGGTTGTTTGATGTATGTAGGTGTTTGGATTATGCATTGTCACTTGGATGTTCACATTGGATGGGGTTTGGCTACTGTGTTTTTGGTAGAGGATGGAGAAGGGCTATTGGAGTCCATAGAAGCTCCTCCTGAGGACCTGCCTTTGTGTTAG
Protein sequence

>MS.gene29699.t1

MEAFNTIFLTIFLLLASLANAKVHEHEFVVQATPVKRLCNTQMSITVNGQFPGPTLEINNGDTLVVKVINKARYNVTIHWHGVRQIRTGWADGPEFVTQCPIRPGESYTYRFTINGQEGTLWWHAHSSWLRATVYGALIIHPKEGDAYPFTKPKRETPILLGEWWDANPMDVVRQATQTGAAPNISDAYTINGQPGDLYKCSTKGTTIVPIDSGETNLLRVINAALNQPLFFTIANHKLTVVGADASYVKPFTTNVLMLGPGQTTDVLIQGDQPPSRYYIAGRAYQSAQNAAFDNTTTTAILEYKSSPCPAKGGANIRPIMPSLPAYNDTNTVTSFSKSFRSLRNVEVPNEIDEDLFFTIGLGLNNCPPNFNANQCQGPNGTRFTSSMNNVSFVLPSNFSILQAHKLGVQGVFTTDFPTKPPVQFDYTGNVSRSLWQPIQGTKVTKLRFGSRVQVVLQDTSIVTPENHPIHLHGYDFYIVAEGFGNYDPKKDASKFNLVDPPMRNTVAVPVNGWAVIRFVADNPGVWIMHCHLDVHIGWGLATVFLVEDGEGLLESIEAPPEDLPLC