Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene30605.t1 | XP_024628620.1 | 74.8 | 329 | 40 | 2 | 1 | 295 | 1 | 320 | 7.50E-125 | 456.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene30605.t1 | Q9FHE1 | 37.1 | 116 | 67 | 3 | 44 | 158 | 260 | 370 | 6.0e-14 | 79.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene30605.t1 | G7JIQ3 | 69.9 | 329 | 56 | 2 | 1 | 295 | 1 | 320 | 1.6e-113 | 418.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene30605 | MS.gene029093 | PPI |
| MS.gene30605 | MS.gene066439 | PPI |
| MS.gene30605 | MS.gene90825 | PPI |
| MS.gene30605 | MS.gene97346 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene30605.t1 | MTR_4g083510 | 72.948 | 329 | 46 | 2 | 1 | 295 | 1 | 320 | 2.11e-160 | 449 |
| MS.gene30605.t1 | MTR_0867s0010 | 65.854 | 328 | 70 | 2 | 1 | 295 | 1 | 319 | 2.25e-142 | 404 |
| MS.gene30605.t1 | MTR_4g063670 | 62.500 | 328 | 81 | 2 | 1 | 295 | 1 | 319 | 5.79e-133 | 380 |
| MS.gene30605.t1 | MTR_1g051185 | 53.000 | 300 | 74 | 2 | 1 | 267 | 1 | 266 | 7.69e-90 | 268 |
| MS.gene30605.t1 | MTR_8g015750 | 67.380 | 187 | 42 | 2 | 127 | 295 | 1 | 186 | 1.20e-72 | 222 |
| MS.gene30605.t1 | MTR_3g069460 | 62.112 | 161 | 52 | 1 | 1 | 161 | 1 | 152 | 5.13e-65 | 202 |
| MS.gene30605.t1 | MTR_7g088160 | 35.952 | 331 | 140 | 11 | 20 | 295 | 75 | 388 | 1.18e-43 | 153 |
| MS.gene30605.t1 | MTR_8g015740 | 69.231 | 91 | 19 | 1 | 1 | 91 | 49 | 130 | 8.37e-35 | 123 |
| MS.gene30605.t1 | MTR_2g023560 | 80.645 | 62 | 12 | 0 | 55 | 116 | 57 | 118 | 2.21e-29 | 108 |
| MS.gene30605.t1 | MTR_7g107340 | 31.660 | 259 | 114 | 9 | 78 | 295 | 98 | 334 | 1.50e-20 | 90.5 |
| MS.gene30605.t1 | MTR_5g044920 | 73.469 | 49 | 4 | 1 | 43 | 91 | 17 | 56 | 4.61e-15 | 70.1 |
| MS.gene30605.t1 | MTR_7g114170 | 27.402 | 281 | 147 | 10 | 18 | 294 | 61 | 288 | 5.29e-13 | 68.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene30605.t1 | AT5G41220 | 42.105 | 95 | 53 | 2 | 65 | 158 | 219 | 312 | 4.89e-17 | 81.6 |
| MS.gene30605.t1 | AT5G41220 | 42.105 | 95 | 53 | 2 | 65 | 158 | 277 | 370 | 4.89e-17 | 81.6 |
| MS.gene30605.t1 | AT5G41220 | 42.105 | 95 | 53 | 2 | 65 | 158 | 227 | 320 | 5.03e-17 | 81.6 |
| MS.gene30605.t1 | AT3G47680 | 34.043 | 141 | 84 | 4 | 30 | 169 | 23 | 155 | 1.13e-15 | 76.3 |
| MS.gene30605.t1 | AT5G41240 | 32.824 | 131 | 75 | 5 | 39 | 161 | 250 | 375 | 3.95e-13 | 70.1 |
Find 46 sgRNAs with CRISPR-Local
Find 66 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAATCTCTTGATCATTTAAT+TGG | 0.210422 | 3.3:+41997387 | None:intergenic |
| ATCTTTCAAAGCATCAAATT+TGG | 0.233493 | 3.3:+41996701 | None:intergenic |
| CCAACAAACTTTGAAATAGA+TGG | 0.279570 | 3.3:+41997050 | None:intergenic |
| TTCAGCTTCTGGAGCATATT+CGG | 0.312536 | 3.3:-41996835 | MS.gene30605:CDS |
| CTTTGATCATTCCCAGCAAT+TGG | 0.326809 | 3.3:+41997185 | None:intergenic |
| TGAACAAAGAATTCAGCTTC+TGG | 0.340604 | 3.3:-41996846 | MS.gene30605:intron |
| CATCTATTTCAAAGTTTGTT+GGG | 0.369114 | 3.3:-41997049 | MS.gene30605:CDS |
| AGAAAACCAGAGGCACTTAA+AGG | 0.378046 | 3.3:-41997095 | MS.gene30605:CDS |
| ATTTCAAAGGATCCAATTGC+TGG | 0.382636 | 3.3:-41997197 | MS.gene30605:CDS |
| CATTGACCTTTAAGTGCCTC+TGG | 0.389323 | 3.3:+41997089 | None:intergenic |
| TCGTTGAAAAGGCGGCAAAA+AGG | 0.398541 | 3.3:-41996758 | MS.gene30605:CDS |
| TCATAATCTTGTAGTTGTCT+TGG | 0.400714 | 3.3:+41996495 | None:intergenic |
| ATGAGAAGTTCATCCTCCTT+TGG | 0.406782 | 3.3:+41997236 | None:intergenic |
| GGTGGATTGTATTCGCTTGT+TGG | 0.416563 | 3.3:+41996792 | None:intergenic |
| GGCTTGGAAACAATTAATCT+TGG | 0.423881 | 3.3:-41997338 | MS.gene30605:CDS |
| CCATCTATTTCAAAGTTTGT+TGG | 0.437206 | 3.3:-41997050 | MS.gene30605:CDS |
| TCCAAGCCATCTTGAGTGCT+AGG | 0.443687 | 3.3:+41997353 | None:intergenic |
| ACCTAGCACTCAAGATGGCT+TGG | 0.469488 | 3.3:-41997354 | MS.gene30605:CDS |
| TATTCGCTTGTTGGTGTTGG+TGG | 0.480363 | 3.3:+41996801 | None:intergenic |
| ACAACGTCCAATCGTTGAAA+AGG | 0.487434 | 3.3:-41996769 | MS.gene30605:CDS |
| ATGAACTTCTCATTCAATCA+TGG | 0.496152 | 3.3:-41997226 | MS.gene30605:CDS |
| CATTTGAACTTCATGAGACA+TGG | 0.503228 | 3.3:+41997424 | None:intergenic |
| CTTGAAAATTCTTGTCGCGA+TGG | 0.504105 | 3.3:+41997120 | None:intergenic |
| GAAGTTCAAATGAATAATAG+TGG | 0.510306 | 3.3:-41997413 | MS.gene30605:CDS |
| TTGTATTCGCTTGTTGGTGT+TGG | 0.511913 | 3.3:+41996798 | None:intergenic |
| AATGGAGATAGAGAGAAAGA+AGG | 0.517552 | 3.3:-41996610 | MS.gene30605:CDS |
| AGGCGAAATGTTGTCGCTCT+CGG | 0.523259 | 3.3:+41996986 | None:intergenic |
| CAGAGGCACTTAAAGGTCAA+TGG | 0.527405 | 3.3:-41997088 | MS.gene30605:CDS |
| TTTCAAAGCATCAAATTTGG+CGG | 0.540641 | 3.3:+41996704 | None:intergenic |
| ATCATGGCTCAATATTTCAA+AGG | 0.547059 | 3.3:-41997210 | MS.gene30605:CDS |
| TTTCAAAGGATCCAATTGCT+GGG | 0.561475 | 3.3:-41997196 | MS.gene30605:CDS |
| CCATCTTGAGTGCTAGGTTG+TGG | 0.563802 | 3.3:+41997359 | None:intergenic |
| ATTCGCTTGTTGGTGTTGGT+GGG | 0.567081 | 3.3:+41996802 | None:intergenic |
| TCAACGATTGGACGTTGTGG+TGG | 0.567689 | 3.3:+41996774 | None:intergenic |
| ATTAATCTTGGTGAAGAAGT+CGG | 0.569606 | 3.3:-41997326 | MS.gene30605:CDS |
| CCACAACCTAGCACTCAAGA+TGG | 0.581215 | 3.3:-41997359 | MS.gene30605:CDS |
| GGCGTGATTAGAGATAAATA+TGG | 0.592289 | 3.3:-41996462 | MS.gene30605:CDS |
| GTTGTCGCTCTAAAGAAAAG+TGG | 0.594037 | 3.3:-41997014 | MS.gene30605:CDS |
| AATAAGATTTAAACCAAAGG+AGG | 0.598325 | 3.3:-41997249 | MS.gene30605:CDS |
| GAAGGGAAAAGAAAAGGTTG+TGG | 0.599261 | 3.3:-41996736 | MS.gene30605:CDS |
| ACGTCCAATCGTTGAAAAGG+CGG | 0.635597 | 3.3:-41996766 | MS.gene30605:CDS |
| GAAAATAAGATTTAAACCAA+AGG | 0.637971 | 3.3:-41997252 | MS.gene30605:CDS |
| TGAAGAAGTCGGAGCTACAT+CGG | 0.648185 | 3.3:-41997315 | MS.gene30605:CDS |
| CGAAATGTTGTCGCTCTCGG+AGG | 0.658068 | 3.3:+41996989 | None:intergenic |
| CAAGATTATGAATTTCTGTG+TGG | 0.676928 | 3.3:-41996483 | MS.gene30605:CDS |
| ATTGCTGGGAATGATCAAAG+AGG | 0.694512 | 3.3:-41997182 | MS.gene30605:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | GAAAAAAAAATTTCAACCAA+AGG | - | chr3.3:41996595-41996614 | MS.gene30605:CDS | 20.0% |
| !! | GAAAATAAGATTTAAACCAA+AGG | - | chr3.3:41996622-41996641 | MS.gene30605:CDS | 20.0% |
| ! | AAAAAAATTTCAACCAAAGG+AGG | - | chr3.3:41996598-41996617 | MS.gene30605:CDS | 25.0% |
| ! | AATAAGATTTAAACCAAAGG+AGG | - | chr3.3:41996625-41996644 | MS.gene30605:CDS | 25.0% |
| ! | CAATCTCTTGATCATTTAAT+TGG | + | chr3.3:41996490-41996509 | None:intergenic | 25.0% |
| ! | GAAGTTCAAATGAATAATAG+TGG | - | chr3.3:41996461-41996480 | MS.gene30605:CDS | 25.0% |
| ! | TATTGAGAGATGAACTAAAA+TGG | - | chr3.3:41996972-41996991 | MS.gene30605:CDS | 25.0% |
| !! | ATCTTTCAAAGCATCAAATT+TGG | + | chr3.3:41997176-41997195 | None:intergenic | 25.0% |
| !! | CATCTATTTCAAAGTTTGTT+GGG | - | chr3.3:41996825-41996844 | MS.gene30605:CDS | 25.0% |
| !! | TGAATTCTTTGTTCATTTTG+CGG | + | chr3.3:41997023-41997042 | None:intergenic | 25.0% |
| !! | TGGTAATAAATATCTTTTGC+AGG | + | chr3.3:41996911-41996930 | None:intergenic | 25.0% |
| AAAAAGGAAGGGAAAAGAAA+AGG | - | chr3.3:41997132-41997151 | MS.gene30605:CDS | 30.0% | |
| ATCATGGCTCAATATTTCAA+AGG | - | chr3.3:41996664-41996683 | MS.gene30605:CDS | 30.0% | |
| ATGAACTTCTCATTCAATCA+TGG | - | chr3.3:41996648-41996667 | MS.gene30605:CDS | 30.0% | |
| ATTAATCTTGGTGAAGAAGT+CGG | - | chr3.3:41996548-41996567 | MS.gene30605:CDS | 30.0% | |
| CAAGATTATGAATTTCTGTG+TGG | - | chr3.3:41997391-41997410 | MS.gene30605:CDS | 30.0% | |
| CCATCTATTTCAAAGTTTGT+TGG | - | chr3.3:41996824-41996843 | MS.gene30605:CDS | 30.0% | |
| TCATAATCTTGTAGTTGTCT+TGG | + | chr3.3:41997382-41997401 | None:intergenic | 30.0% | |
| ! | ATCAAAGAGGAGATAGTTTT+TGG | - | chr3.3:41996705-41996724 | MS.gene30605:CDS | 30.0% |
| ! | ATTTTCAACTCTTTGCAACT+CGG | + | chr3.3:41997296-41997315 | None:intergenic | 30.0% |
| ! | TTTCAAAGCATCAAATTTGG+CGG | + | chr3.3:41997173-41997192 | None:intergenic | 30.0% |
| !! | CCAACAAACTTTGAAATAGA+TGG | + | chr3.3:41996827-41996846 | None:intergenic | 30.0% |
| !!! | ACGTTTTGAGAGTGAAAAAA+TGG | - | chr3.3:41997246-41997265 | MS.gene30605:CDS | 30.0% |
| AATGGAGATAGAGAGAAAGA+AGG | - | chr3.3:41997264-41997283 | MS.gene30605:CDS | 35.0% | |
| ATTTCAAAGGATCCAATTGC+TGG | - | chr3.3:41996677-41996696 | MS.gene30605:CDS | 35.0% | |
| GGCGTGATTAGAGATAAATA+TGG | - | chr3.3:41997412-41997431 | MS.gene30605:CDS | 35.0% | |
| GGCTTGGAAACAATTAATCT+TGG | - | chr3.3:41996536-41996555 | MS.gene30605:CDS | 35.0% | |
| TGAACAAAGAATTCAGCTTC+TGG | - | chr3.3:41997028-41997047 | MS.gene30605:CDS | 35.0% | |
| TGAATTGTTCACGTACATCT+TGG | + | chr3.3:41996931-41996950 | None:intergenic | 35.0% | |
| TTTCAAAGGATCCAATTGCT+GGG | - | chr3.3:41996678-41996697 | MS.gene30605:CDS | 35.0% | |
| ! | TTCTTTGTTCATTTTGCGGA+CGG | + | chr3.3:41997019-41997038 | None:intergenic | 35.0% |
| !! | GATAGTTTTTGGATGAGGAT+TGG | - | chr3.3:41996716-41996735 | MS.gene30605:CDS | 35.0% |
| !! | TTTTCAAGAGAGAAAACCAG+AGG | - | chr3.3:41996769-41996788 | MS.gene30605:CDS | 35.0% |
| !!! | AATCTTATTTTCGCCTCCTT+TGG | + | chr3.3:41996614-41996633 | None:intergenic | 35.0% |
| ACAACGTCCAATCGTTGAAA+AGG | - | chr3.3:41997105-41997124 | MS.gene30605:CDS | 40.0% | |
| ATGAGAAGTTCATCCTCCTT+TGG | + | chr3.3:41996641-41996660 | None:intergenic | 40.0% | |
| ATTGCTGGGAATGATCAAAG+AGG | - | chr3.3:41996692-41996711 | MS.gene30605:CDS | 40.0% | |
| CTTGAAAATTCTTGTCGCGA+TGG | + | chr3.3:41996757-41996776 | None:intergenic | 40.0% | |
| CTTTGATCATTCCCAGCAAT+TGG | + | chr3.3:41996692-41996711 | None:intergenic | 40.0% | |
| GAAGGGAAAAGAAAAGGTTG+TGG | - | chr3.3:41997138-41997157 | MS.gene30605:CDS | 40.0% | |
| ! | GATGAACTAAAATGGCTTGC+AGG | - | chr3.3:41996980-41996999 | MS.gene30605:CDS | 40.0% |
| ! | TTGTATTCGCTTGTTGGTGT+TGG | + | chr3.3:41997079-41997098 | None:intergenic | 40.0% |
| ! | TTTTCAACGATTGGACGTTG+TGG | + | chr3.3:41997106-41997125 | None:intergenic | 40.0% |
| !! | AGAAAACCAGAGGCACTTAA+AGG | - | chr3.3:41996779-41996798 | MS.gene30605:CDS | 40.0% |
| !! | GAGGAGATAGTTTTTGGATG+AGG | - | chr3.3:41996711-41996730 | MS.gene30605:CDS | 40.0% |
| !! | GTTGTCGCTCTAAAGAAAAG+TGG | - | chr3.3:41996860-41996879 | MS.gene30605:intron | 40.0% |
| !! | TTCAGCTTCTGGAGCATATT+CGG | - | chr3.3:41997039-41997058 | MS.gene30605:CDS | 40.0% |
| ACGTCCAATCGTTGAAAAGG+CGG | - | chr3.3:41997108-41997127 | MS.gene30605:CDS | 45.0% | |
| CATTGACCTTTAAGTGCCTC+TGG | + | chr3.3:41996788-41996807 | None:intergenic | 45.0% | |
| GAAAAGGCGGCAAAAAGGAA+GGG | - | chr3.3:41997121-41997140 | MS.gene30605:CDS | 45.0% | |
| TCGTTGAAAAGGCGGCAAAA+AGG | - | chr3.3:41997116-41997135 | MS.gene30605:CDS | 45.0% | |
| TGAAAAGGCGGCAAAAAGGA+AGG | - | chr3.3:41997120-41997139 | MS.gene30605:CDS | 45.0% | |
| TGAAGAAGTCGGAGCTACAT+CGG | - | chr3.3:41996559-41996578 | MS.gene30605:CDS | 45.0% | |
| ! | ATGGCTTGCAGGTTGTGTAT+TGG | - | chr3.3:41996991-41997010 | MS.gene30605:CDS | 45.0% |
| ! | TTTGCCGCCTTTTCAACGAT+TGG | + | chr3.3:41997115-41997134 | None:intergenic | 45.0% |
| !! | ATTCGCTTGTTGGTGTTGGT+GGG | + | chr3.3:41997075-41997094 | None:intergenic | 45.0% |
| !! | CAGAGGCACTTAAAGGTCAA+TGG | - | chr3.3:41996786-41996805 | MS.gene30605:CDS | 45.0% |
| !! | GGTGGATTGTATTCGCTTGT+TGG | + | chr3.3:41997085-41997104 | None:intergenic | 45.0% |
| !! | TATTCGCTTGTTGGTGTTGG+TGG | + | chr3.3:41997076-41997095 | None:intergenic | 45.0% |
| ACCTAGCACTCAAGATGGCT+TGG | - | chr3.3:41996520-41996539 | MS.gene30605:CDS | 50.0% | |
| AGGCGAAATGTTGTCGCTCT+CGG | + | chr3.3:41996891-41996910 | None:intergenic | 50.0% | |
| CCACAACCTAGCACTCAAGA+TGG | - | chr3.3:41996515-41996534 | MS.gene30605:CDS | 50.0% | |
| CCATCTTGAGTGCTAGGTTG+TGG | + | chr3.3:41996518-41996537 | None:intergenic | 50.0% | |
| TCAACGATTGGACGTTGTGG+TGG | + | chr3.3:41997103-41997122 | None:intergenic | 50.0% | |
| TCCAAGCCATCTTGAGTGCT+AGG | + | chr3.3:41996524-41996543 | None:intergenic | 50.0% | |
| CGAAATGTTGTCGCTCTCGG+AGG | + | chr3.3:41996888-41996907 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.3 | gene | 41996452 | 41997444 | 41996452 | ID=MS.gene30605 |
| chr3.3 | mRNA | 41996452 | 41997444 | 41996452 | ID=MS.gene30605.t1;Parent=MS.gene30605 |
| chr3.3 | exon | 41996962 | 41997444 | 41996962 | ID=MS.gene30605.t1.exon1;Parent=MS.gene30605.t1 |
| chr3.3 | CDS | 41996962 | 41997444 | 41996962 | ID=cds.MS.gene30605.t1;Parent=MS.gene30605.t1 |
| chr3.3 | exon | 41996452 | 41996859 | 41996452 | ID=MS.gene30605.t1.exon2;Parent=MS.gene30605.t1 |
| chr3.3 | CDS | 41996452 | 41996859 | 41996452 | ID=cds.MS.gene30605.t1;Parent=MS.gene30605.t1 |
| Gene Sequence |
| Protein sequence |
